CN108477612A - A kind of dendrobium nobile nutritive powder and preparation method including dendrobium candidum purified extract - Google Patents
A kind of dendrobium nobile nutritive powder and preparation method including dendrobium candidum purified extract Download PDFInfo
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- CN108477612A CN108477612A CN201810117982.0A CN201810117982A CN108477612A CN 108477612 A CN108477612 A CN 108477612A CN 201810117982 A CN201810117982 A CN 201810117982A CN 108477612 A CN108477612 A CN 108477612A
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- dendrobium
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- dendrophnol
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention provides a kind of dendrobium nobile nutritive powders and preparation method thereof including dendrobium candidum purified extract, wherein the nutrition powder particles have nucleocapsid body, and include at least：The amino-acid modified slow-digestion starch of dendrobium candidum, dendrobium candidum purified polysaccharide and dendrophnol.The dendrobium nobile nutritive powder of the present invention is suitble to the various crowds including hyperglycemia edible, and there is good hypoglycemic effect and health-nutrition to be worth.
Description
Technical field
Blood glucose, dendrobium nobile nutritive powder comprising dendrobium candidum purified extract and preparation side are reduced the present invention relates to a kind of
Method, relate more specifically to a kind of nucleocapsid comprising dendrobium candidum specific polysaccharide component, amino acid and dendrophnol extract
Dendrobium nobile nutritive powder and preparation method thereof can be reconstituted, blood sugar reducing food field and nutrient and healthcare products field are belonged to.
Background technology
The stem of noble dendrobium (scientific name：Dendrobium nobile Lindl), also known as celestial dry measure used in former times orchid rhythm, not dead grass, whorlleaf stonecrop herb, purple entangle celestial being
Strain, Lin Lan, prohibition of life, nobile flower etc..Medicinal plant, property slightly sweet flavor is micro- salty, trembles with fear, returns stomach, kidney, lung channel.Reinforcing stomach reg fluid, enriching yin are clear
Heat.Impairment of yin body fluid deficiency, dry polydipsia it have been usually used in since being used as medicine, deficiency of food is retched, and abnormal heat, mesh are secretly unknown after being ill.It is traditional as China
Rare traditional Chinese medicine, medicinal dendrobium include the fresh stem of noble dendrobium, HERBA DENDROBII, dendrobium candidum, dendrobium loddigesii Rolfe, stem of Eyeshaped Dendrobium etc.；2010 editions medicines
Allusion quotation starts " dendrobium candidum " independent list, is the fresh of orchid family Dendrobium Sw dendrobium candidum or dry stem.2010 editions pharmacopeia
Start the fresh or dry stem for being divided into three kinds of plant HERBA DENDROBIIs, Dendrobium Chrysotoxum Lindl, Dendrobium fimbriatum Hook under " stem of noble dendrobium " item.In addition common
Medicinal dendrobium also has Dendrobidium huoshanness, is commonly called as a meter dry measure used in former times；Dendrobium devonianum, also Dendrobium loddigesii, Dendrobium chrysanthum etc..
Research medicine shows that the stem of noble dendrobium has high medicinal and healthcare function, therefore has obtained deeply extensive research.It grinds
Study carefully and shows that its effective component includes：Dendrobium polysaccharide, stem of noble dendrobium amino acid, alkaloid, flavonoids etc..Its main pharmacodynamics ingredient is the stem of noble dendrobium
Polysaccharide, is one kind of active polysaccharide, and dendrobium polysaccharide is the wider a kind of heteroglycan of molecular weight distribution.Heteroglycan is two kinds or two kinds
The polysaccharide of the above difference monosaccharide molecule composition.It is had shown that through research, dendrobium polysaccharide can significantly reduce the blood glucose value of body, with oral drop
Blood glucose medicine such as sulfonylurea is different, it acts on euglycemia without reduction, does not also improve serum insulin level, and to pancreas islet
Cell has repair；Dendrobium officinale polysaccharide also has the function of hypoglycemic outside pancreas simultaneously, by inhibiting adrenaline to cause
Hepatic glycogenolytic and promote hepatic glycogen synthesis, so as to reduce the source of blood glucose, increase the outlet of blood glucose, reach reduction blood glucose
The purpose of value.
Pretty wait of Zhejiang University Wu Hao has carried out special experimental study to dendrobium candidum hypoglycemic effect and its mechanism.It selects
Normal mouse, the disorderly property hyperglycemia mouse of adrenal gland, streptozotocin diabetes rat are as experimental subjects, from blood glucose value, blood
Clear insulin and pancreas islet blood glucose element level are started with, using the methods of radiommunoassay and immunohistochemistry HRP-SPA chromosomes, knot
The morphological change etc. for closing alpha Cell of islet and β cells has affirmed that dendrobium candidum has apparent hypoglycemic effect.It is dropped
Mechanism is to promote islet β cell insulin in the pancreas of blood glucose, and alpha Cell of islet is inhibited to secrete glucagon, the outer mechanism of pancreas
It is to promote hepatic glycogen synthesis.This research provides pharmacology foundation for dendrobium candidum for treating diabetes, and makees for dendrobium candidum
Wide foreground is illustrated for hypoglycemic drug exploitation.
As it can be seen that in above-mentioned medicinal dendrobium, dendrobium candidum contains higher high-quality polyoses content.
Further multinomial the study found that polysaccharide is the main component of dendrobium candidum, water-soluble polysaccharide content is up to 22%
More than.The power of stem of noble dendrobium physiological activity is closely related with its polyoses content.Polyoses content is higher, and matter is heavier, the more toughness chewed,
Quality is more excellent.In the different parts of dendrobium candidum, the mass fractions of total starches is followed successively by stem from high to low, on stem, under stem, root
Portion.But the water-soluble polysaccharide regularity of distribution is leaf >=root ＞ stems.Modern study is found, after the water-soluble polysaccharide of dendrobium candidum protocorm
It was found that it is mainly made of mannose, rhamnose, galacturonic acid, glucose, galactolipin and arabinose, molecular substance
The ratio between amount is 1.1204：1：1.046：23.354：3.828：1.046.Wherein, after extracting water-soluble polysaccharide, wherein glucose accounts for
According to huge part.
Therefore, directly that dendrobium candidum milling or polyoses extract is directly edible to be expected to reduce blood glucose in the prior art
Way often leads to absorb the component that a large amount of glucose etc. easily leads to hyperglycemia, greatly reduces the medicinal effect of dendrobium polysaccharide
Fruit, even without corresponding effect.This is also one of the dendrobium polysaccharide class health products prior art drawback of the invention to be overcome.
In the prior art, although the pharmacological activity of medicinal dendrobium polysaccharide and chemical constitution research achieve many progress,
But in terms of pharmacology activity research, used polysaccharide is mainly Thick many candies, about grinding for the medicinal dendrobium active polysaccharide mechanism of action
Study carefully and is not much；In terms of chemical constitution research, much the polysaccharide components with pharmacological activity, chemical constitution are not yet clear.And
And medicinal or food-grade dendrobium polysaccharide is isolated and purified there are still various deficiencies, such as recovery rate are low, purity is not high, journey
Sequence is cumbersome, unsuitable industrialized production, and waste of raw materials is serious etc..
In addition, the stem of noble dendrobium equally has good effect for diabetic complications and complication.For example, the dendrophnol of the stem of noble dendrobium
Ingredient helps to improve diabetes complicated cataract.
Dendrophnol (Gigantol), a kind of bibenzyl phenolic substance ((4- (3- hydroxy-5-methyl oxygroups phenethyl) -2- methoxies
Base phenol), it is one of effective medicinal components of the stem of noble dendrobium.Have now been found that some evidences illustrate the stem of noble dendrobium and live with various biological
Property, including enhancing immunity of organisms, help gastric digestion, anti-cataract, adjust blood glucose, it is antitumor the effects that.By to the stem of noble dendrobium
Phenol mechanism study finds that dendrophnol can block by the table of lipopolysaccharide-induced nitric oxide synthase type and cyclooxygenase
It reaches, is the active ingredient of anti-cataract in the stem of noble dendrobium, it can effectively inhibit AR (aldose reductase), improve diabetic's polyalcohol
Metabolic pathway is abnormal, prevents and postpone the occurrence and development of diabetes and its complication cataract.
Chen Dan etc. (《Traditional Chinese Medicine University Of Guangzhou》, 2014) the study found that the dendrophnol of a concentration of 120 μm of ol/L to iNOS's
Inhibiting rate is 100%, and half amount of suppression (IC50) is (32.227 ± 5.73) μm ol/L；Dendrophnol passes through to iNOS active sites
The direct effect of point, and then inhibit the generation of NO, it is lle195, Gln257 that the two, which acts on amino acid sites, experiments have shown that the stem of noble dendrobium
Phenol has good inhibiting effect to iNOS, can improve and repair diabetic's polyalcohol metabolic pathway, prevents and delay is sugared
Urine disease and its complication.
The beneficial key component of other in the stem of noble dendrobium further includes amino acid.Amino acid in the stem of noble dendrobium is mainly deposited in a free form
In addition to polysaccharide, the content of amino acid will be far above other compositions.Free amino acid be also in the stem of noble dendrobium it is main effectively at
/ mono-.Experiments have shown that dendrobium candidum contains whole essential amino acids in addition to tryptophan, wherein primary amino acid is
Aspartic acid, glutamic acid, glycine, valine and leucine, this 5 kinds of amino acids account for 53% of total amino acid or so.Wherein, paddy
The content of propylhomoserin, aspartic acid and glycine accounts for 35.8% of total content or so.
At present, it has been disclosed that more extraction process and preparation product to the stem of noble dendrobium, such as：
CN104784505A discloses a kind of Dendrobium officinale health product of auxiliary hyperglycemic and preparation method thereof, by weight
Number meter, weighs the following raw material component, 35~45 parts of the fresh item of dendrobium candidum, 1~2 part of American Ginseng, 2~4 parts of pueraria lobata, root of Chinese trichosanthes 2~4
1~2 part of part and Schisandra chinensis.Preparation method includes the following steps：Raw material weighs, the processing of the fresh item of dendrobium candidum, American Ginseng
Processing, alcohol extracting, water carry, dry and seven steps of dispensing are made.The raw material prescription Chinese medicine enriching yin boost qi, clearing heat and promoting fluid, medicine
Object active ingredient is easy to absorb, and is conducive to play drug effect；Each medicinal material is integration of drinking and medicinal herbs medicinal material, nontoxic pair in the raw material prescription
Effect.
CN104126746A discloses a kind of hypoglycemic, reducing blood lipid dendrobium candidum composition and preparation method thereof, by with
The Chinese medicine material medicine of lower weight proportion is made：10-40 parts of dendrobium candidum, 10-30 parts of Chinese yam, 5-35 parts of cassia seed, sealwort 10-30
Part, 10-30 parts of sealwort, 10-30 parts of oat extract, 10-20 parts of maltodextrin, 2-10 parts of soyabean oligosaccharides.The present invention's is excellent
Putting is：The invention comprehensively utilizes dendrobium candidum, Chinese yam, cassia seed, sealwort, avenabeta glucosan, soyabean oligosaccharides etc. are a variety of
Raw material, and it is aided with appropriate maltodextrin, it is prepared into the composition for reducing blood glucose and blood fat.Dendrobium candidum, Chinese yam, Cassia
Son, sealwort, oat contain polysaccharose substance, and be proven reduces blood glucose, reduces blood fat and treat glycosuria with preferable
Disease and other effects.
CN104958564A discloses a kind of dendrobium candidum oral solution and preparation method thereof, and dendrobium candidum oral solution is by iron sheet
The stem of noble dendrobium, arillus longan and mulberry fruit, which add water to cook, to be process, per 1000mL dendrobium candidums oral solution by 90~100g dendrobium candidums, 90
~100g arillus longans and 90~100g mulberry fruits form through adding water to cook post-processing.Preparation side of the raw material through repeatedly decocting in the present invention
Method, active ingredient that can be in high efficiency extraction medicinal material, three kinds of dendrobium candidum, arillus longan and mulberry fruit components are pressed in dendrobium candidum oral solution
Ratio is allocated, energy reinvigoration, enriching yin help, and the natural plant polyose substance contained in component can enhance immune function,
Without sucrose in dendrobium candidum oral solution, without any additive, the equal edible of hyperglycemia people with hyperlipidemia.
CN104324244A discloses a kind of compound dendrobium officinale Jiangtang capsule, using dendrobium candidum be aided with Radix Astragali, pueraria lobata,
Radix Rehmanniae, root of Chinese trichosanthes, sealwort, the fruit of Chinese wolfberry, 7 taste Chinese medicine of radix paeoniae rubra, hypoglycemic glue is prepared using crushing, extraction, mixing, granulation, packaging
Capsule plays the role of influencing each other, and collaboration is made altogether；It can promote islet β cell insulin, alpha Cell of islet is inhibited to secrete pancreas
Glucagons, while can inhibit hepatic glycogenolytic caused by adrenaline and promoting hepatic glycogen synthesis, to reduce blood glucose source,
Increase blood glucose outlet, reduce blood glucose value, have no toxic side effect and adverse reaction, " three-many-one-little " symptom and glycosuria of diabetes can be made
Sick complication is effectively controlled and eliminates.
But the above-mentioned full powder of the prior art generally use stem of noble dendrobium, as described above, being contained in its full powder many to hypoglycemic
Unhelpful component.Even if minority is extracted polysaccharide, but after prior art extraction polysaccharide component, usually gives up defective material waste residue, in
Amino acid also slattern together, it is very uneconomical.
Total amino acid average mass fraction is 7.43% in the blade of 11 strains of dendrobium candidum, stem 1.91%, blade
It is significantly higher than stem, and amino acid composition close to the standard of the FAO/WHO ideal proteins proposed there is good functional food to open
Hair foreground.
There are many extracting method about free amino acid, including the extraction of ultrasonic extraction, zymohydrolysis extracting method, high-pressure water heating
Method, solvent extraction method, microwave loss mechanisms etc..There are high-pressure water heating extraction, ultrasonic extraction, enzymolysis to carry using more at present
It follows the example of, but the recovery rate of free amino acid and extraction process are still to be improved, especially the extraction process report of stem of noble dendrobium amino acid
Road is less, does not achieve the purpose that synthesis makes full use of stem of noble dendrobium active principle.
Moreover, it is noted that although dendrobine in the full powder of the stem of noble dendrobium has analgesic, refrigeration function, can reduce heart rate,
Blood pressure slows down breathing, but can generate the hyperglycemia of moderate.In addition, dendrobine is not suitable for excessive use as alkaloids.
Dendrobine is equal in such as HERBA DENDROBII, dendrobium candidum, cerasite dry measure used in former times, the clavus stem of noble dendrobium, the big luxuriant sheath stem of noble dendrobium, dendrobium moniliformeSweet, nobile-type dendrobium
It is distributed.Contain dendrobine mostly in the full powder of the stem of noble dendrobium currently on the market, this is especially unhelpful to hyperglycemia patient, especially
Excessive use is easy aggravation blood glucose rise when often taking.Therefore, for hyperglycemia patient, having in the separation and Extraction stem of noble dendrobium
Beneficial component is simultaneously aided with suitable dosage form, and there is an urgent need.
In the hypoglycemic dendrobium health-care product composition of currently available technology, existing another question is without suitable
Matching vector.On the one hand, when taking other Traditional Chinese medicine for reducing blood glucose component extracts of combination, not only mouthfeel is poor, such as bitter, puckery etc.,
And the amount that control is taken is generally required, excessive use causes hypoglycemia even to be fainted；On the other hand, non-Pharmaceutical ingredients are combined
When, such as Chinese yam, cereal etc., the intake of excessive starch or carbohydrate is often led to, these substances decompose generation in vivo
It thanks and improves blood glucose level indirectly, effect is caused to be had a greatly reduced quality, therefore, for matching the suitable carrier or group of dendrobium polysaccharide
Point, there is also active demands.
Slow-digestion starch (slowly digestible starch, SDS), referring to those can be inhaled in small intestine by complete digestion
Receipts but slow starch (20-120min)；Clinical research in recent years find, slow-digestion starch have slowly digest and assimilate,
Continuous energy release maintains postprandial blood sugar stable state, improves glucose tolerance, can also reduce Postprandial insulin secretion, improve body
Sensibility, the various special physiological functions with diet chronic diseases of prevention and treatment to insulin.Slow-digestion starch
As a kind of novel functional product, diabetes nutrition is taken into account and has cured two big demand of function, not only complied with contemporary food
Product and the trend of drug development in science and technology, also meet the health demand of consumer, this is to rationally utilizing carbohydrate to prevent pancreas
Important in inhibiting occurs for the relevant metabolic syndrome of insulin resistance.Therefore, numerous researchers are all conceived to slow digestion and form sediment
The research of powder.Such as application No. is 201110112582.9 patents, and alcohol soluble protein is put in beaker, and ethyl alcohol is added, water-bath is stirred
It mixes to alcohol soluble protein and is completely dissolved, oleic acid is added, stirs evenly, cornstarch is added, continues to stir.In high pressure homogenizer
Matter 3 times, centrifugal spray drying obtains slow-digestion starch product.Application No. is 201310058866.3 patents to use Pullulanase
Wheaten starch is handled with glycosyltransferase, slow-digestion starch is prepared in the method by recrystallizing repeatedly.
As described above, although the prior art provides various slow-digestion starch preparation methods, what is prepared disappears slowly
It is still not high enough to change content of starch, and effect and component are single.
Invention content
As described above, to overcome the shortcomings of hypoglycemic stem of noble dendrobium food compositions in the prior art, the present invention is intended to provide a kind of
Nucleocapsid, the dendrobium candidum nutrient powder and preparation method thereof that can reduce blood glucose with good taste.
In addition, preparation method of the present invention is simple, it is suitble to large-scale production, product is easy to carry, and stability is good, the resting period
It is long, it both can directly reconstitute and drink, and also can be used as the addO-on therapy of food processing, purposes is very extensive.
Specifically, the invention mainly relates to following aspects.
The first aspect, the present invention provides it is a kind of reduce blood glucose, include the stem of noble dendrobium of dendrobium candidum purified extract
Nutrient powder, wherein the nutrient powder has nucleocapsid structure body, and includes at least：The amino-acid modified slow digestion of dendrobium candidum is formed sediment
Powder, dendrobium candidum purified polysaccharide and dendrophnol.
Wherein, the dendrobium candidum purified polysaccharide is the specific polysaccharide component of the method for the present invention purifying, is beneficial to reduce
Blood glucose.The dendrophnol can be commercially available food-grade dendrophnol sterling, can also be the finished product prepared by the method for the present invention.
The second aspect, the present invention provides the preparation method of above-mentioned dendrobium candidum nutrient powder, the preparation method includes
Following preparation process：
(1) free amino acid is extracted from dendrobium candidum leaf；
(2) modified slow-digestion starch is prepared with the dendrobium candidum amino acid of extraction；
(3) Dendrobium officinale polysaccharide component is extracted and purifies, the polysaccharide component relative molecular weight is more than 150KDa；
(4) it is extracted from the dendrobium candidum degreasing wasting liquid of extraction polysaccharide step and purifies dendrophnol；
(5) it prepares slurry and builds nutrition powder particles, wherein the Dendrobium officinale polysaccharide is with dendrophnol mass ratio
100:0.1-0.5。
Wherein, in the preparation method of nutrient powder of the present invention, step (1) is prepared as follows：(a) it will clean and pass through
Dendrobium candidum blade powder after water-removing dries pulverizing sieves with 100 mesh sieve, and the distilled water of 10-40 times of quality is added, and is vibrated in water-bath
20-30min is extracted at 50-60 DEG C in device, after extraction, leaching liquor is depressurized to suction filtration while hot, filtrate is collected and is cooled to
Room temperature；The absolute ethyl alcohol that 0.5-1 times of volume is added into filtrate removes residual protein, purification process 6-18h at 4 DEG C, with 8000-
9000r/min centrifuges 15-20min, takes supernatant, filters, and filtrate decompression concentration is dried in vacuo to obtain amino acid at 50-60 DEG C
Crude product；The extraction content of free amine group acid crude is 35-50mg/g；
(b) the 50-60% ethyl alcohol of 6-8 times of quality of above-mentioned coarse amino acid is dissolved, concentration is tied using being evaporated in vacuo
Crystalline substance obtains refined amino acid crystals, and purity is 99% or more.
After measured, primary amino acid is aspartic acid, glutamic acid, glycine, valine and leucine in gained crystal, is accounted for
The half or so of total amino acid weight.
Wherein it is preferred to by the further recrystallization purifying of amino acid crystals obtained.
In preparation method of the present invention, the amino-acid modified slow-digestion starch of dendrobium candidum is prepared in step (2)
It is handled using the hydro-thermal method of no enzyme and soda acid additive, on the basis of amino acid content is constant, passes through further modification
To promote slow-digestion starch content, specific preparation process is as follows：
(a) slow-digestion starch is dissolved in distilled water, 20~40% slow-digestion starch lotions are made in stirring at room temperature；
(b) the free amine group acid crystal extracted from dendrobium candidum leaf is added into above-mentioned slow-digestion starch breast, at room temperature
20~30min of magnetic agitation, drying is to moisture in 50-60%；
(c) above-mentioned steps products therefrom is put into sealed thermal insulating reactor 10~16h of isothermal holding at 55~60 DEG C；
(d) reactant is taken out, cooled to room temperature, is dried under vacuum to moisture and is less than 10%, crushes, sieving,
Up to the slow-digestion starch that dendrobium candidum free amino acid is modified, measure wherein modified slow-digestion starch content 78% with
On.
Wherein, the slow-digestion starch can be commercially available, can also be obtained by this field common preparation method, excellent
Select slow-digestion starch content in 40% or more (based on Englyst methods).
The slow-digestion starch source can digest shallow lake slowly for any natural food materials source in this field, such as corn
Powder, wheat slow-digestion starch or konjaku slow-digestion starch etc..
Wherein, amino acid is added in above-mentioned steps and the ratio of slow-digestion starch is：The slow-digestion starch dry weight and ammonia
The mass ratio of base acid crystal is 20~30:1.
In preparation method of the present invention, the specific step of Dendrobium officinale polysaccharide component is extracted and purified in step (3)
It is rapid as follows：
(a) dendrobium polysaccharide crude product is prepared：Dendrobium candidum stem micro mist is crossed into 60 mesh sieve, with the food-grade 95% of 8-10 times of quality
After ethyl alcohol impregnates 30min, flow back degreasing 1-1.5h；By the distillation water dissolution of 15-20 times of quality of degreasing powder product, then water
The cellulase degradation 1-2h of addition stem of noble dendrobium dry powder quality 1-2% when bath is warming up to 50-55 DEG C, destroys cell wall, fully discharges
Polysaccharide component；Then enzymolysis liquid is heated to 90-95 DEG C, high temperature enzyme deactivation, and 30-60min is kept the temperature, at interval of 5min using ultrasound
1-2min is extracted, filtrate is collected by filtration；By filter residue plus 10 times of quality distilled water, it is heated to 90-95 DEG C, and keep the temperature 40-60min,
At interval of 5min ultrasonic extraction 1-2min, filtering, filtrate is concentrated into ten/one by merging filtrate through rotavapor under vacuum
3-4 times of volume absolute ethyl alcohol is added into above-mentioned concentrate for product, and 12-24 hours precipitate polysaccharides, mistake are stood in 0-4 DEG C or ice bath
Filter is used in combination 75% food grade ethyl alcohol to wash precipitation, and precipitation drying, which crushes, is made protein-contg polysaccharide crude；Take above-mentioned polysaccharide
Extract crude product is dissolved in 20-50 times of quality water, and the Sevag reagent (isoamyl alcohol of 20-25% volume ratios is added：Chloroform is 1：4 match
Make), after ultrasonic vibration 3-5min, 3000-4000r/min centrifuges 15-20min under room temperature, takes supernatant to repeat above-mentioned super
Acoustic shock swings-centrifugally operated 3-4 times, until de- albumen is complete；Merge supernatant, 3-4 times of volume ethanol is added, ice bath places 10-12
Hour, filtering is collected sediment and is washed with absolute ethyl alcohol, solvent is evaporated off, is dried in vacuo to obtain dendrobium polysaccharide crude product；
(b) purified polysaccharide：
It takes above-mentioned dendrobium polysaccharide crude product soluble in water, monosaccharide is removed with dialysis membrane (molecular cut off 4000Da) dialysis 24-48h
And after oligosaccharides, takes out liquid in bag filter and centrifuge 10-15min, concentrated supernatant through 10000-12000rpm, freeze-drying obtains
Obtain dendrobium polysaccharide prefabrication；By the propylene glucan column chromatography (Sephacryl of above-mentioned polysaccharide prefabrication apparatus molecular sieving effect
S-200 it) or by the DEAE-52 cellulose ion exchange columns of NaCl stepwise elutions is isolated and purified, collects main peak component, closed
And the polysaccharide component (accounting for 73% of total reducing sugar or more) of 150KDa or more is concentrated, it (is 70- to ethanol content that ethanol in proper amount, which is added,
75%), ice bath places 12h, and filtering is collected sediment and washed with absolute ethyl alcohol, and pellet frozen drying is made of the present invention
Dendrobium officinale polysaccharide fine work, the gained polysaccharide products yield 13.8% in terms of raw material.Polyoses content is measured through Phenol sulfuric acid procedure
Albumen is measured below 0.5% for 95% or more, bradford methods.
Wherein, the polysaccharide crude can also use commercially available dendrobium candidum polysaccharide extractive, and using the present invention
Purification process is further purified.
In preparation method of the present invention, extracts in step (4) and purify dendrophnol and be as follows：
The ethyl alcohol extracted component that the above-mentioned reflux defatting step prepared in dendrobium polysaccharide is given up is collected, is concentrated under reduced pressure, with just
Hexane extracts 2-3 times, then is extracted 3-4 times with ethyl acetate, and filtrate is evaporated off and obtains dendrophnol coarse extract；It is added suitable 20%
Sodium carbonate liquor is stirred to react 2-3 hours, is placed 4-12 hours, and high speed centrifugation collects supernatant, and concentration is filled with pure titanium dioxide
Carbonating 1-2 hours obtains white flock precipitate, and centrifuging and taking precipitates, and twice wash with distilled water；Ethyl alcohol is added to proper in precipitation
It is completely dissolved well, adsorbing separation is carried out with reverse phase silica gel column on 2-3BV/h, with water-ethanol system gradient elution, thin-layered chromatography
Tracing detection collects dendrophnol section eluent, dendrophnol crude product is concentrated under reduced pressure to obtain, is crystallized with absolute ethyl alcohol, and recrystallize 2-3
It is secondary, white dendrophnol crystal is obtained, recovery rate is 0.5mg/g in terms of stem of noble dendrobium dry powder.
Wherein, the dendrophnol coarse extract and the quality usage ratio of 20% sodium carbonate liquor are 1：10-20.
In preparation method of the present invention, structure nutrition powder particles are as follows in step (5)：
(a) starch emulsion that distilled water prepares 15-30wt% is added in the amino-acid modified slow-digestion starch of the stem of noble dendrobium of preparation,
It is at the uniform velocity stirred until homogeneous on magnetic stirring apparatus, the stem of noble dendrobium made from addition trehalose, gellan gum and above-mentioned steps is more while stirring
Sugar, dendrophnol use homogenizer homogeneous, wherein mixed dendrobium polysaccharide, dendrophnol, trehalose and gellan gum after stirring evenly
Content be：Dendrobium polysaccharide 5-15wt%, trehalose 2-3wt%, gellan gum 0.1wt%, dendrophnol content are dendrobium polysaccharide
0.1-0.5%；By the mixed liquor electrostatic spray drying after homogeneous, gel particle powder is made；
(b) suitable hypromellose, stevioside, chitosan are added into the sodium alginate soln of 3-5wt%,
Homogeneous stirs evenly, and viscous syrup is made, wherein the mass percentage of each raw material is：Sodium alginate 3-5wt%, hydroxypropyl
Cellulose 1-3wt%, stevioside 0.1-0.3wt%, chitosan 0.1-0.2wt%；The viscosity is starched with spraying drying machine
Even application is expected on above-mentioned gel particle powder surface, to which the dendrobium nutrient powder particles be made.
Wherein, the spraying dosage of the viscous syrup is the one third of gel particle powder weight to half.
The third aspect of the invention is provided through dendrobium nobile nutritive powder made from above-mentioned preparation method.
The fourth aspect of the invention provides the purposes of nutrient powder, and the product that reconstitute can be used as directly edible, can also be made
For the additive of food processing, the working process process of staple food is used it for.
The fifth aspect of the invention provides a kind of food, health products or pharmaceutical composition, which is characterized in that described
Food, health products or pharmaceutical composition include dendrobium nutrient powder particles of the present invention.
In the nutrient powder of the present invention, it is related to the " comprising " of composition, had both contained open "include", "comprise"
Deng and its similar meaning, also contain enclosed " Consists of " etc. and its similar meaning.
Beneficial effects of the present invention further include but are not limited to following：
Herba Dendrobii micropowder compared with the existing technology or polysaccharide crude composition, eliminate the monosaccharide such as unnecessary glucose/
The unwanted components such as oligosaccharides, alkaloid with blood glucose increasing effect, purifying, which concentrates, has simultaneously screened beneficial polysaccharide component, is aided with rationally
The amino-acid modified high-content slow-digestion starch of the dendrophnol component and the stem of noble dendrobium to improve diabetic complication of ratio,
Stem of noble dendrobium raw material are fully efficiently utilized, synergistic improvement blood glucose has been achieved the purpose that in effect, has been not only suitable for hyperglycemia people
Group is daily to take with hypoglycemic, due to the mechanism that polysaccharide especially dendrophnol does not stimulate insulin secretion, also is adapted for general population
Daily consumption is to reach the purpose of preventing diabetes, obesity.
In addition, preparation method of the present invention is simple, it is suitble to large-scale production, has a good application prospect and market value.
Specific implementation mode
Below by specific preparation example and embodiment, the present invention is described in detail, but these exemplary embodiments
Purposes and purpose be only used for enumerating the present invention, any type of any limit not is constituted to the real protection scope of the present invention
It is fixed, it is more non-that protection scope of the present invention is confined to this.
Preparation example 1：Free amino acid is extracted from dendrobium candidum leaf
(1) 50 grams of the dendrobium candidum blade powder cleaned and after the dries pulverizing that finishes is sieved with 100 mesh sieve, 1500ml is added
Distilled water extracts 30min in water bath chader at 60 DEG C, and after extraction, leaching liquor is depressurized to suction filtration while hot, collects filter
Liquid 30ml is simultaneously cooled to room temperature；
(2) absolute ethyl alcohol that 0.5 times of volume is added into filtrate removes residual protein, purification process 12h at 4 DEG C, with
8000r/min centrifuges 20min, takes supernatant, the nanofiltration membrane for being 1000Da with molecular cut off, filtrate decompression concentration,
2.5 grams of coarse amino acid is dried in vacuo to obtain at 50 DEG C；The extraction content of free amine group acid crude is 50mg/g；
(3) above-mentioned coarse amino acid 50% ethyl alcohol of 20ml is dissolved, concentration using vacuum evaporating crystalization, obtains essence
The amino acid crystals of system measure its purity 99% or more.
Preparation example 2：Prepare the amino-acid modified slow-digestion starch of dendrobium candidum
(1) 100 grams of konjaku slow-digestion starch are dissolved in distilled water, stirring at room temperature is made 20~40% slow digestion and forms sediment
Powder lotion；5 grams of the free amine group acid crystal extracted in the above-mentioned leaf from dendrobium candidum, room temperature are added into above-mentioned slow-digestion starch breast
Lower magnetic agitation 30min, drying is to moisture 50%；
(2) above-mentioned steps products therefrom is put into sealed thermal insulating reactor the isothermal holding 16h at 55 DEG C；
(3) reactant is taken out, cooled to room temperature, is dried under vacuum to moisture and is less than 10%, crushes, sieving,
Up to the slow-digestion starch that dendrobium candidum free amino acid is modified, wherein modified slow-digestion starch is measured to obtain by Englyst methods
Content is about 82%.
Preparation example 3：It extracts and purifies Dendrobium officinale polysaccharide component
(1) dendrobium polysaccharide crude product is prepared：100 grams of 60 mesh of mistake sieves of clean dendrobium candidum stem micro mist are weighed, are eaten with 1000ml
After 95% ethyl alcohol of grade impregnates 30min, flow back degreasing 1.5h；By the distilled water of 15 times of quality of degreasing powder product after drying
It dissolves, then warming-in-water destroys cell wall, fully to the cellulase degradation 2h for adding stem of noble dendrobium dry powder quality 2% at 55 DEG C
Discharge polysaccharide component；Then enzymolysis liquid is heated to 95 DEG C, high temperature enzyme deactivation, and 60min is kept the temperature, it is carried using ultrasound at interval of 5min
1min is taken, filtrate is collected by filtration；By filter residue plus 10 times of quality distilled water, 90 DEG C are heated to, and keep the temperature 40min, at interval of 5min
Ultrasonic extraction 2min, filtering, filtrate is concentrated into 1/10th volumes by merging filtrate through rotavapor under vacuum, to above-mentioned dense
3 times of volume absolute ethyl alcohols are added in contracting liquid, 18 hours precipitate polysaccharides are stood in 0-4 DEG C or ice bath, filtering be used in combination 75% to eat
Grade ethyl alcohol washing precipitation, precipitation drying, which crushes, is made protein-contg polysaccharide crude；
(2) above-mentioned polyoses extract crude product is taken to be dissolved in 20 times of quality water, the Sevag reagents that 20% volume ratio is added are (different
Amylalcohol：Chloroform is 1：4 are formulated), after ultrasonic vibration 5min, 4000r/min centrifuges 20min under room temperature, and supernatant is taken to repeat
Above-mentioned ultrasonic vibration-centrifugally operated 3 times, until de- albumen is complete；Merge supernatant, 4 times of volume ethanols is added, it is small that ice bath places 12
When, filtering is collected sediment and is washed with absolute ethyl alcohol, solvent is evaporated off, is dried in vacuo to obtain dendrobium polysaccharide crude product；
(3) purified polysaccharide：
It takes above-mentioned except the dendrobium polysaccharide crude product after deproteinized is soluble in water, is dialysed with the dialysis membrane of molecular cut off 4000Da
Monosaccharide and oligosaccharides are removed for 24 hours, are taken out liquid in bag filter and are centrifuged 15min, concentrated supernatant through 12000rpm, freeze-drying obtains
Obtain dendrobium polysaccharide prefabrication；
(4) by the propylene glucan column chromatography (Sephacryl S-200) of above-mentioned polysaccharide prefabrication apparatus molecular sieving effect
Or isolated and purified by the DEAE-52 cellulose ion exchange columns of NaCl stepwise elutions, main peak component is collected, concentration is merged
The polysaccharide component of 150KDa or more, it is 70% that ethanol in proper amount to ethanol content, which is added, and ice bath places 12h, and sediment is collected in filtering
It is used in combination absolute ethyl alcohol to wash, the Dendrobium officinale polysaccharide fine work is made in pellet frozen drying, the gained polysaccharide essence in terms of raw material
Product yield 13.8%.It is that 97%, bradford methods measure protein content 0.4% to measure polyoses content through Phenol sulfuric acid procedure.
Preparation example 4：It extracts and purifies dendrophnol
The ethyl alcohol extraction solution for collecting the defatting step that flows back in above-mentioned preparation example 3, is concentrated under reduced pressure into 50ml, is extracted with n-hexane
It takes 2-3 times, then is extracted 3-4 times with ethyl acetate, filtrate is evaporated off and obtains 5 grams of dendrophnol coarse extract；50 grams of 20% carbon is added
Acid sodium solution is stirred to react 3 hours, is placed 12 hours, and 6000r/min high speed centrifugations collect supernatant, and concentration is filled with pure two
Oxidation carbonating 1 hour obtains white flock precipitate, and centrifuging and taking precipitates, and twice wash with distilled water；Ethyl alcohol is added extremely in precipitation
Just it is completely dissolved, adsorbing separation is carried out with reverse phase silica gel column on 3BV/h, with water-ethanol system gradient elution, thin-layered chromatography
Tracing detection collects dendrophnol section eluent, dendrophnol crude product is concentrated under reduced pressure to obtain, is crystallized with absolute ethyl alcohol, and recrystallizes 2 times,
White dendrophnol crystal 50.3mg is obtained, purity 99%, recovery rate is 0.5mg/g in terms of stem of noble dendrobium dry powder.
Preparation example 5：Build nutrition powder particles
(1) by 100 grams of starch milks that distilled water is added and prepares 20wt% of the amino-acid modified slow-digestion starch of the stem of noble dendrobium of preparation
500 grams of liquid, is at the uniform velocity stirred until homogeneous on magnetic stirring apparatus, and suitable trehalose, gellan gum and above-mentioned step are added while stirring
Rapid dendrobium polysaccharide, dendrophnol obtained, use homogenizer homogeneous, wherein mixed dendrobium polysaccharide, dendrophnol, sea after stirring evenly
Algae sugar and the content of gellan gum are：Dendrobium polysaccharide 10wt%, trehalose 2wt%, gellan gum 0.1wt%, dendrophnol content are
0.01wt%；By the mixed liquor electrostatic spray drying after homogeneous, gel particle powder is made；
(2) suitable hypromellose, stevioside, chitosan are added into the sodium alginate soln of 3wt%,
Matter stirs evenly, and 500 grams of viscous syrup is made, wherein the mass percentage of each raw material is：Sodium alginate 3wt%, hydroxypropyl first
Base cellulose 1wt%, stevioside 0.1wt%, chitosan 0.2wt%；The viscous syrup is uniformly sprayed with spraying drying machine
It is coated in the above-mentioned gel particle powder surface quickly rolled, is dried in vacuo, sterilizing, to which the dendrobium nobile nutritive powder be made
Grain.
Comparative example 1：Extract and purify the Dendrobium officinale polysaccharide component of 3-10KDa
In addition to purifying and collecting the Dendrobium officinale polysaccharide component in the sections 3-10KDa, according to identical method in preparation example 3
Implement comparative example 1.
Comparative example 2：Extract and purify the Dendrobium officinale polysaccharide component of 10-150KDa
In addition to purifying and collecting the Dendrobium officinale polysaccharide component in the sections 10-150KDa, according to identical side in preparation example 3
Method implements comparative example 2.
Effect example 1：Hypoglycemic test
Test method：Healthy male KM mouse is chosen, mouse is randomly divided into 5 by weight 20g (upper and lower variance rate 10%)
Group：Blank control group, model control group and 3 groups tested group；The polysaccharide component for wherein giving tested material group is respectively：Preparation example
3 polysaccharide components, 1 polysaccharide component of comparative example, 2 polysaccharide component of comparative example, every group of experimental animal 10, experiment periods are 30 days.
Diabetes mice model foundation：Mouse fasting 12h, disposable celiac inject streptozotocin 15mg/100g weight,
Mouse fasting 12h, survey fasting blood sugar are the successful diabetes model of modeling higher than 11mmol/L person after a week for modeling.
Wherein, blank control group：The daily gavage equivalent distilled water of normal mouse；
Model control group：The daily gavage equivalent distilled water of diabetic mice；
Tested group：Daily with 120mg/kg.bw polysaccharide component gavage diabetic mices.
Numerical value is averaged, and experimental result is as follows：
Table 1
As seen from the above table, compared with model control group, polysaccharide component (being more than 150KDa) can significantly reduce in preparation example 3
Diabetic mice fasting blood glucose level and Area under the curve of blood glucose；Polysaccharide component (10-150KDa) is to a certain extent in comparative example 2
Diabetic mice fasting blood glucose level can be reduced；Polysaccharide component (3-10KDa) is only capable of slight decrease diabetic mice in comparative example 1
Fasting blood glucose level, effect are very little.
The experimental result illustrates that the dendrobium polysaccharide component of 150KDa or more is to diabetic mice relative to low-molecular-weight polysaccharide
With apparent hypoglycemic effect.It is possible thereby to prove that the nutrition powder particles of the present invention have blood sugar decreasing effect to diabetes, and
Diabetic complication can be improved.
It should be appreciated that the purposes of these embodiments is merely to illustrate the present invention and is not intended to limitation protection model of the invention
It encloses.In addition, it should also be understood that, after reading the technical contents of the present invention, those skilled in the art can make the present invention each
Kind change, modification and/or variation, all these equivalent forms equally fall within and are protected defined by the application the appended claims
Within the scope of shield.
Claims (9)
1. a kind of preparation method of the dendrobium nobile nutritive powder comprising dendrobium candidum purified extract, the preparation method includes following step
Suddenly：
(1) free amino acid is extracted from dendrobium candidum leaf；
(2) modified slow-digestion starch is prepared with the dendrobium candidum free amino acid of extraction；
(3) Dendrobium officinale polysaccharide component is extracted and purifies, the polysaccharide component relative molecular weight is more than 150KDa；
(4) it is extracted from the dendrobium candidum degreaser of extraction polysaccharide step and purifies dendrophnol；
(5) it prepares slurry and builds dendrobium nutrient powder particles, wherein the Dendrobium officinale polysaccharide is with dendrophnol mass ratio
100:0.1-0.5。
2. preparation method as described in claim 1, it is characterised in that：The step (1) is prepared as follows：
(a) the dendrobium candidum blade powder cleaned and after the dries pulverizing that finishes is sieved with 100 mesh sieve, 10-40 times of quality of addition
Distilled water extracts 20-30min in water bath chader at 50-60 DEG C, and after extraction, leaching liquor is depressurized to suction filtration while hot,
It collects filtrate and is cooled to room temperature；The absolute ethyl alcohol that 0.5-1 times of volume is added into filtrate removes residual protein, is purified at 4 DEG C
6-18h is handled, 15-20min is centrifuged with 8000-9000r/min, takes supernatant, is filtered, filtrate decompression concentration, at 50-60 DEG C
It is dried in vacuo to obtain coarse amino acid；The extraction content of free amine group acid crude is 35-50mg/g；
(b) the 50-60% ethyl alcohol of 6-8 times of quality of above-mentioned coarse amino acid is dissolved, concentration using vacuum evaporating crystalization, obtains
To refined amino acid crystals.
3. preparation method as described in claim 1, it is characterised in that：The step (2) is prepared as follows：
(1) slow-digestion starch is dissolved in distilled water, the slow-digestion starch of mass fraction 20~40% is made in stirring at room temperature
Lotion；
(2) the free amine group acid crystal extracted from dendrobium candidum leaf is added into above-mentioned slow-digestion starch breast, at room temperature magnetic force
20~30min is stirred, drying is to moisture in 50-60%；
(3) above-mentioned steps products therefrom is put into sealed thermal insulating reactor 10~16h of isothermal holding at 55~60 DEG C；
(4) reactant is taken out, cooled to room temperature, be dried under vacuum to moisture be less than 10%, crush, sieving to get
The slow-digestion starch that dendrobium candidum free amino acid is modified.
4. preparation method as described in claim 1, it is characterised in that：The step (3) is prepared as follows：
(1) dendrobium polysaccharide crude product is prepared：Dendrobium candidum stem micro mist is crossed into 60 mesh sieve, with 95% ethyl alcohol of food-grade of 8-10 times of quality
After impregnating 30min, flow back degreasing 1-1.5h；By the distillation water dissolution of 15-20 times of quality of degreasing powder product, then water-bath liter
Temperature destroys cell wall, fully discharges polysaccharide to the cellulase degradation 1-2h for adding the stem of noble dendrobium dry powder quality 1-2% at 50-55 DEG C
Component；Then enzymolysis liquid is heated to 90-95 DEG C, high temperature enzyme deactivation, and 30-60min is kept the temperature, use ultrasonic extraction at interval of 5min
Filtrate is collected by filtration in 1-2min；By filter residue plus 10 times of quality distilled water, it is heated to 90-95 DEG C, and keep the temperature 40-60min, every
Every 5min ultrasonic extraction 1-2min, filtering, filtrate is concentrated into 1/10th volumes by merging filtrate through rotavapor under vacuum,
3-4 times of volume absolute ethyl alcohol is added into above-mentioned concentrate, stands 12-24 hours precipitate polysaccharides in 0-4 DEG C or ice bath, filters,
It is used in combination 75% food grade ethyl alcohol to wash precipitation, precipitation drying, which crushes, is made protein-contg polysaccharide crude；Above-mentioned polysaccharide is taken to carry
It takes object crude product to be dissolved in 20-50 times of quality water, the Sevag reagent (isoamyl alcohol of 20-25% volume ratios is added：Chloroform is 1：4 prepare
Into), after ultrasonic vibration 3-5min, 3000-4000r/min centrifuges 15-20min under room temperature, and supernatant is taken to repeat above-mentioned ultrasound
Concussion-centrifugally operated 3-4 times；Merge supernatant, 3-4 times of volume ethanol is added, ice bath is placed 10-12 hours, filtering, and it is heavy to collect
Starch is simultaneously washed with absolute ethyl alcohol, and solvent is evaporated off, and is dried in vacuo to obtain dendrobium polysaccharide crude product；
(2) purified polysaccharide：
It takes above-mentioned dendrobium polysaccharide crude product soluble in water, monosaccharide and widow is removed with the dialysis membrane of molecular cut off 4000Da dialysis 24-48h
After sugar, takes out liquid in bag filter and centrifuge 10-15min, concentrated supernatant through 10000-12000rpm, freeze-drying obtains stone
Dry measure used in former times polysaccharide prefabrication；It is washed by the propylene glucan column chromatography of above-mentioned polysaccharide prefabrication apparatus molecular sieving effect or by the NaCl stages
De- DEAE-52 cellulose ion exchange columns are isolated and purified, and main peak component is collected, and merge the polysaccharide of concentration 150KDa or more
Ethanol in proper amount is added in component, and ice bath places 12h, and filtering is collected sediment and washed with absolute ethyl alcohol, pellet frozen drying, system
Obtain the Dendrobium officinale polysaccharide fine work.
5. preparation method as described in claim 1, it is characterised in that：The step (4) is prepared as follows：
The ethyl alcohol extracted component for collecting the above-mentioned reflux defatting step prepared in dendrobium polysaccharide, is concentrated under reduced pressure, with n-hexane extraction
2-3 times, then extracted 3-4 times with ethyl acetate, filtrate is evaporated off and obtains dendrophnol coarse extract；It is molten that suitable 20% sodium carbonate is added
Liquid is stirred to react 2-3 hours, is placed 4-12 hours, and high speed centrifugation collects supernatant, and concentration is filled with pure carbon dioxide acidification 1-
2 hours, white flock precipitate is obtained, centrifuging and taking precipitates, and twice wash with distilled water；Will precipitation be added ethyl alcohol to just completely it is molten
Solution carries out adsorbing separation with reverse phase silica gel column on 2-3BV/h, with water-ethanol system gradient elution, thin-layered chromatography tracking inspection
It surveys, collects dendrophnol section eluent, dendrophnol crude product is concentrated under reduced pressure to obtain, is crystallized with absolute ethyl alcohol, and recrystallize 2-3 times, obtains white
Color dendrophnol crystal；
Wherein it is preferred to which the quality usage ratio of the dendrophnol coarse extract and sodium carbonate liquor is 1：10-20.
6. preparation method as described in claim 1, it is characterised in that：The step (5) is prepared as follows：
(1) starch emulsion that distilled water prepares 15-30wt%, magnetic force is added in the amino-acid modified slow-digestion starch of the stem of noble dendrobium of preparation
It is at the uniform velocity stirred until homogeneous on blender, while stirring dendrobium polysaccharide, stone made from addition trehalose, gellan gum and above-mentioned steps
Dry measure used in former times phenol uses homogenizer homogeneous after stirring evenly, wherein mixed dendrobium polysaccharide, dendrophnol, trehalose and gellan gum contain
Amount is：Dendrobium polysaccharide 5-15wt%, trehalose 2-3wt%, gellan gum 0.1wt%, dendrophnol dosage are the 0.1- of dendrobium polysaccharide
0.5wt%；By the mixed liquor electrostatic spray drying after homogeneous, gel particle powder is made；
(2) suitable hypromellose, stevioside, chitosan, homogeneous are added into the sodium alginate soln of 3-5wt%
It stirs evenly, viscous syrup is made, wherein the mass percentage of each raw material is：Sodium alginate 3-5wt%, hydroxypropyl methylcellulose
Plain 1-3wt%, stevioside 0.1-0.3wt%, chitosan 0.1-0.2wt%；It is with spraying drying machine that the viscous syrup is equal
It is even to be sprayed on above-mentioned gel particle powder surface, to which the dendrobium nobile nutritive powder be made.
7. according to dendrobium nobile nutritive powder made from any one of the claim 1-6 preparation methods.
8. the food, health products containing dendrobium nobile nutritive powder described in claim 7 or pharmaceutical composition.
9. the purposes of dendrobium nobile nutritive powder as claimed in claim 7, which is characterized in that being used to prepare reduces diabetes blood glucose's level
Preparation or product.
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