CN102988827B - Dendrobium officinale granula - Google Patents
Dendrobium officinale granula Download PDFInfo
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Abstract
The invention discloses a dendrobium officinale granula. The dendrobium officinale granula mainly comprises dendrobium officinale, poria cocos, ginseng, fructus lycii, rhizoma dioscoreae and semen coicis, wherein the ratio of the dendrobium officinale to poria cocos to ginseng to fructus lycii to rhizoma dioscoreae to semen coicis by weight is 15 to 28: 30 to 42: 7 to 18: 3 to 13: 15 to 28: 15 to 28. The dendrobium officinale granula has the effects of adjusting the immunologic function, resisting the fatigue and boosting Qi and nourishing Yin, is suitable for weak people and easily fatigable, specially shows good effect for people with rehabilitation, overwork and pneumogastric deficiency of Yin, can remove the physically and mentally fatigued feel of people due to busy work and study, and also can effectively improve the working efficiency as well as learning efficiency.
Description
Technical field
The present invention relates to technical field of Chinese medicines, relate in particular to a kind of Chinese medicine granules of the effect with enhancing immunity, alleviating physical fatigue being formed by kinds of traditional Chinese medicines materials such as Herba Dendrobii.
Background technology
Chinese medicine granules refers to that the extract of Chinese crude drug and proper auxiliary materials or part medical material fine powder mix, the dry graininess dosage form of making.Chinese medicine granules is a kind of form of Chinese drug growing up in conjunction with the feature of decoction, medicated wine and syrup, both kept decoction to absorb fast, effective feature rapidly, overcome again decoction and taken front interim decoction, be long placed in the easy mould rotten feature that loses, also can cover the bitterness of some Chinese medicine.Also can say " Chinese medicine granules is prepared slices of Chinese crude drugs processing processing procedure and form improvement progress of research; be according to Chinese medical theory clinical practice needs, and a kind of novel form increasing evident in efficacy that is easy to carry and takes that Chinese crude drug and tcm and herbal slice are carried out to special processing ".Start to come across the seventies in last century, because it carries taking convenience, in the Chinese medicine manufacture of the eighties in last century, once developed to increase progressively 41.9% speed year.
The extraction of Chinese medicinal granule, concentrated, dry, adjuvant selection, method of granulating and granularity selection etc. all should carry out preferred and detailed research.Due to Chinese medicine ingredients complexity, should embody Chinese medical theory instruct self-character, also to retain the Close relation of the rational essence of Chinese medicine conventional formulation, attention composition and curative effect etc., so the problem of the preparation key of Chinese medicine granules is the technique of extracting and dry technology research to crude drug.
Extraction is the unit operations of separating effective ingredient from medicinal raw material, is directly connected to the content of product effective ingredient, affects the enforcement of inherent quality, clinical efficacy, economic benefit and GMP.The research of Chinese medicine preparation and produce from the ball of the former powder molding of conventional formulation, fall apart to lixiviate type preparation as the rise and development of granule, extractum sheet, capsule, oral liquid, injection etc., be the feature of half a century Chinese medicine preparation progress, belong to from conventional formulation and enter the period of improving preparation.Due to Chinese medicine ingredients complexity, embody the self-character that Chinese medical theory instructs, retain as far as possible the rational own job of Chinese medicine conventional formulation, the aspects such as the Close relation of emphasis composition and curative effect, the research of extracting technique of Chinese medicine and selection are very important, are also quite complicated problems.
Herba Dendrobii is the dry stem of orchid Herba Dendrobii Dendrobium officinale Kimura et Migo, sweet in the mouth, and cold nature, returns stomach, kidney channel.There is reinforcing stomach reg fluid, the function of nourishing YIN and clearing away heat.Modern medicine and pharmacology research shows, Herba Dendrobii mainly contains the chemical compositions such as polysaccharide, aminoacid and trace element, luxuriant and rich with fragrance class and a small amount of alkaloid, has the pharmacological actions such as the immunity of enhancing, resisting fatigue, antitumor, blood sugar lowering.
Poria is On Polyporaceae Poria Poria cocos(Schw.) dry sclerotia of Wolf, sweet in the mouth, light, property is flat, GUIXIN, lung, spleen, kidney channel.There is promoting diuresis to eliminate damp pathogen, the function of spleen invigorating mind calming.Modern medicine and pharmacology research shows, Poria main component is β-pachyman and three mushrooms compound second phthalein pachymic acid, pachymic acid and 3 beta-hydroxy Pilus Caprae seu Ovis steroid trienic acids.There is the functions such as the immunity of raising, antitumor, anti-liver cirrhosis.
Radix Ginseng is the dry root and rhizome of Araliaceae Radix Ginseng Panax ginseng C.A.Mey., sweet in the mouth, micro-hardship, and property is flat, returns spleen, lung, heart channel.Have strongly invigorating primordial QI, multiple arteries and veins is admittedly de-, and invigorating the spleen to benefit the lung, promotes the production of body fluid, the function of calming the nerves.Modern medicine and pharmacology research shows, Radix Ginseng is mainly containing compositions such as multiple ginsenoside, saccharide, volatile oil, peptide, aminoacid, trace element and organic acid, multivitamin, choline and enzymes.There is resisting fatigue, enhancing immunity, antitumor, blood sugar lowering and the anti-ageing effect of waiting for a long time.
Fructus Lycii is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L., sweet in the mouth, and property is flat, returns liver, kidney channel.There is nourishing the liver and kidney, the function of replenishing vital essence to improve eyesight.Modern medicine and pharmacology research shows, Fructus Lycii mainly contains the chemical compositions such as saccharide, aminoacid and trace element, has the functions such as enhancing immunity, antitumor, blood fat reducing.
Rhizoma Dioscoreae is the dry rhizome of Dioscoreaceae plant Rhizoma Dioscoreae Dioscorea opposite Thumb., sweet in the mouth, and property is flat, returns spleen, lung, kidney channel.There is spleen reinforcing nourishing the stomach, the lung benefiting that promotes the production of body fluid, the function of the kidney invigorating arresting seminal emission.Modern medicine and pharmacology research shows, Rhizoma Dioscoreae contains polysaccharide, protein, dopamine, hydrochloric acid Rhizoma Dioscoreae alkali, batatasins, vitamin, calcium, magnesium, copper and abundant iodine and other trace element, and the chemical composition such as allantoin, soap former times, choline.There is the function of blood sugar lowering, enhancing immunity, defying age and blood fat reducing.
Semen Coicis is grass Semen Coicis Coix lacryma-jobi L.var.mayuen(Roman.) dry mature kernal of Stapf, sweet in the mouth, light, cool in nature, return spleen, stomach, lung meridian.There is spleen invigorating eliminating dampness by diuresis, eliminating impediment antidiarrheal, the function of clearing away heat and discharging pus.Modern medicine and pharmacology research shows, Semen Coicis contains saccharide, coixenolide (coixenolide), and myristic acid (myristic acid), campesterol etc., still containing aminoacid, protein etc.There is the functions such as enhancing immunity, antitumor, blood sugar lowering.
But, up to the present, there is not yet the report of the Chinese medicine preparation taking Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae and Semen Coicis as main component.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of Herba Dendrobii granule is provided.Herba Dendrobii granule of the present invention can enhancing immunity, alleviating physical fatigue.
The object of the invention is to be achieved through the following technical solutions: a kind of Herba Dendrobii granule, it is mainly made up of Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae and Semen Coicis, wherein, the weight proportion of described Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis is: 15～28:30～42:7～18:3～13:15～28:15～28.
This Herba Dendrobii granule prepares by following technique:
1, according to the weight proportion of Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis be: draw materials in 15～28:30～42:7～18:3～13:15～28:15～28.
2, Herba Dendrobii is put in multi-function extractor, added 20 times of water gagings and soak 4h, decoct 1.5h, filter to obtain the first filtering residue and the first filtrate;
3, the first filtering residue, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae and Semen Coicis are put in multi-function extractor, added 12 times of water gagings and decoct 1h, filter to obtain the second filtering residue and the second filtrate;
4, the second filtering residue is put in multi-function extractor, added 10 times of water gagings and decoct 1h, filter to obtain the 3rd filtering residue and the 3rd filtrate;
5, merge the first filtrate, the second filtrate and the 3rd filtrate, after filtering with flame filter press, filtrate is placed in concentrating under reduced pressure tank; Under 55-65 DEG C, vacuum 0.07-0.09MPa, being evaporated to relative density is 1.15-1.20(60 DEG C of survey), obtain concentrated extract;
6, in concentrated extract, adding quality is concentrated extract 1.5-2.5 beta-schardinger dextrin-doubly, is spray dried to extract powder.
7, dry granulation, the granulate that sieves obtains Herba Dendrobii granule.
The present invention has following technique effect: regulate immunologic function, resisting fatigue, boosting qi and nourishing yin, be applicable to have a delicate constitution and fatiguability person, especially to rehabilitation after being ill, overwork, lung-stomach yin deficiency shower has good effect, eliminate people by the spiritlessness and weakness due to busy working and learning, can effectively improve working and learning efficiency.
Traditional decoction method Herba Dendrobii effective component extraction rate is lower, because the crude polysaccharides content in Herba Dendrobii is more, and in medical material, contain a large amount of phlegmatic temperaments, each composition is more difficult fried, and method of the present invention is carried out the immersion of certain hour before extraction, many extractions are once all conducive to the extraction of its active ingredient separately; Utilize the advanced technologies granulation agent such as spraying is dry, dry granulation.
The present invention adopts granule dosage form, taking beta-schardinger dextrin-as excipient, there is the features such as dissolubility is good, the good bioavailability of mouthfeel is high, easy to carry, accurate measurement, end product has the effect of strengthening the spleen and nourishing the stomach, nourishing the liver and kidney, boosting qi and nourishing yin, strengthening the body resistance, prove the health food of tool enhancing immunity, alleviating physical fatigue through scientific experiments, there is good wide market prospect.
Carry out toxicological safety experiment with Herba Dendrobii granule of the present invention:
1, acute toxicity testing conclusion: adopt maximum tolerated dose method, Herba Dendrobii granule of the present invention is contaminated to each 10 the per os gavages of male and female mice (19-22g) with 20000mg/kg dosage.Gavage capacity is pressed 0.4ml/20g weighing machine, in 24 hours to mouse stomach 2 times.The maximum tolerated dose MTD of the acute oral of Herba Dendrobii granule of the present invention to male and female white mice is all greater than 20000mg/kg.
2, micronucleus test conclusion: adopt 30 hours twice administration by gavage, by Herba Dendrobii granule of the present invention respectively with 10000mg/kg, 5000mg/kg, tri-dosage groups of 2500mg/kg, to mice (25-30g, male and female half and half), press 0.4ml/20g body weight, respectively to each treated animal gavage.Herba Dendrobii granule bone marrow polychromatic erythrocyte micronucleus test result of the present invention is negative.
3, sperm deformity experiment conclusion: sample, respectively with 10000mg/kg, 5000mg/kg, tri-dosage groups of 2500mg/kg, is pressed to 0.4ml/20g body weight to male mice (25-35g), respectively to each treated animal gavage.Once a day, continuous 5 days.In giving first after sample the 35th day, get two side epididymis and shred, get filtrate film-making.Microscopy is observed, the lopsided number of 1000 sperms of every Mus counting.Herba Dendrobii granule sperm deformity experimental result of the present invention is negative.。
4, Ames experiment conclusion: adopt flat board to mix method, test and measuring is 0.06mg/ ware, 0.18mg/ ware, 0.55mg/ ware, 1.66mg/ ware, 5.00mg/ ware.Detection repeats once.Herba Dendrobii granule Ames experimental result of the present invention is negative.
5, within 30 days, feed experiment: 80 of SD rats (60-80g), male and female half and half, set up 2.5g/kg, 5.0g/kg, tri-dosage groups of 10.0g/kg (to be equivalent to 25 times of Herba Dendrobii granule recommended dose 6g/kg/ day of the present invention Herba Dendrobii granule of the present invention, 50 times, 100 times) single cage raising, free diet, feeds 30 days continuously.Result Growth in Rats situation is substantially good.The check result of hematochrome, erythrocyte, numeration of leukocyte and classification thereof is all within normal range.The histological examination result of the main organs such as liver,kidney,spleen, gastrointestinal, sexual organ is not found and tests relevant pathological changes.
Carry out the experiment of relevant enhancing immunity function and anti-fatigue effect with Herba Dendrobii granule of the present invention
Herba Dendrobii granule of the present invention, is light yellow granule, and human body recommended dose is 6g/60kg every day.Basic, normal, high three dosage groups are established in this zoopery, i.e. 0.5g/kg, 1.0g/kg, 3.0g/kg is equivalent to respectively 5,10,30 times of human body recommended dose.Give sample 30 days to the continuous gavage of mice, separately establish blank group, gavage gives distilled water.Experimental result shows: Herba Dendrobii granule of the present invention can obviously strengthen Cell-mediated Immunity, improve monocytes/macrophages phagocytic activity, but humoral immunization activity and NK cytoactive has no obvious enhancing.
Conclusion: under this experimental condition, its mouse oral gives Herba Dendrobii granule of the present invention and has enhancing immunity function.
1. material
1.1, tested Herba Dendrobii granule of the present invention and dosage design: Herba Dendrobii granule of the present invention, this product human body recommended dose is 6g/60kg every day.Basic, normal, high three dosage groups are established in this zoopery, i.e. 0.5g/kg, 1.0g/kg, 3.0g/kg is equivalent to respectively 5,10,30 times of human body recommended dose.Give sample 30 days to the continuous gavage of mice, separately establish blank group, gavage gives distilled water.Take sample 0.5g, 1.0g, 3.0g difference adding distil water to 20ml, be the test liquid of basic, normal, high three dosage groups in this ratio preparation.1.2, laboratory animal: clean level ICR kind white mice, credit number: SCXK(Shanghai) 2007-0005, body weight 21-27g, female, provided by Shanghai Slac Experimental Animal Co., Ltd., this center SPF Animal House is raised.The temperature 20-25 DEG C of laboratory animal breeding room, relative humidity 40-70%, laboratory animal occupancy permit number: SYXK(Shanghai) 2007-0008, animal feeding material is provided by Suzhou two lion laboratory animal feed technology Services Co., Ltd, registration card number: the E of Soviet Union raises new word (2002) 006.
2. test method
2.1, grouping: 200 mices are randomized into large group of five experiments by body weight, every group 40 (immune one group is carried out NK cytoactive detection and pouring turns experiment, immune two groups are carried out DTH, spleen index and thymus index experiment, immune three groups are carried out hemolysis plaque and hemolysin experiment, four groups of immunity are carried out carbon and are cleaned up experiment, engulf experiment for immune five groups).
2.2, feed and weigh: according to dosage design is continuously fed mice 30 days, weighing weekly once, at the beginning of experiment with computing, mid-term (second week) end and experiment opisthosoma weight.Experimental result is added up with SPSS software.
2.3, internal organs/body weight ratio (thymus index, spleen index) is measured: animal is given sample after 30 days continuously, and cervical vertebra dislocation is put to death, and gets thymus, spleen is weighed, and calculates respectively thymus index and spleen index.
2.4, cellular immune function assay:
2.4.1, the mouse spleen lymphocyte transformation experiment of ConA induction: animal gives sample after 30 days continuously, and cervical vertebra dislocation is put to death, and gets spleen, makes splenocyte suspension, and adjusting cell concentration is 3 × 10
6individual/ml, divides two holes to add in 24 well culture plates cell suspension, every hole 1ml, and a hole adds 75 μ l ConA liquid (being equivalent to 7.5 μ g/ml), and 5%CO in contrast, is put in another hole
2, cultivate 72h for 37 DEG C.Cultivation finishes front 4h, and every hole adds MTT(5mg/ml) 50 μ l/ holes, continue to cultivate 4h, after cultivation finishes, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixes, after purple crystal is dissolved completely, carry out colorimetric with 570nm wavelength, experimental result is added up with SPSS15.0.
2.4.2, the mensuration of DTH mensuration and thymus index, spleen index: sensitization: animal gives sample after 30 days continuously, every Mus abdominal part unhairing, the about 3cm × 3cm of scope, is evenly applied in mouse right ear (two sides) by 1%DNFB solution 10 μ l and attacks.After 24h, every Mus is weighed, and mice is put to death in cervical vertebra dislocation, takes off the left and right auricle of diameter 8mm with card punch, weighs and calculates swelling.Get thymus again, spleen is weighed, and calculates respectively thymus index, spleen index.
2.5, humoral immune function is measured:
2.5.1, antibody producing detection test: animal gives sample after 30 days continuously, and after 5 days, animal cervical vertebra dislocation execution, gets spleen, makes splenocyte suspension by the immunity of defiber sheep red blood cell (SRBC), and adjusting cell concentration is 5 × 10
6individual/ml.By after top layer culture medium (1g agarose adds distilled water to 100ml) heating for dissolving, put into 45 DEG C of water bath heat preservations, mix with the Hanks liquid of equivalent pH7.2-7.4,2 times of concentration, subpackage small test tube, every pipe 0.5ml, then add 50 μ l 110%SRBC in pipe, incubation 1.5h in 20 μ l, then join in plate with the complement (1:10) of SA buffer dilution, continue after incubation 1.5h, counting hemolysis plaque number.
2.5.2, serum hemolysin experiment: animal is given sample after 30 days, with 2%(v/v continuously) SRBC immune animal is after 5 days, eye socket blood sampling, separation of serum.On 96 hole Microhemagglutination plates, add 100 μ l normal saline, then add 100 μ l serum at first row respectively, each row makes doubling dilution later, and every hole adds 1%SRBC100 μ l, vibrates latter 37 DEG C and places 3h, observed result after sinking appears in blood cell contrast.
2.6, monocytes/macrophages functional examination experiment:
2.6.1, mice carbon cleans up experiment: animal gives sample after 30 days continuously, and the india ink of tail vein injection 1:3 dilution treats that prepared Chinese ink injects, timing immediately.Inject after prepared Chinese ink 2, after 10min, get blood 20 μ l from vena ophthalmica respectively, and be added to 2ml 0.1%Na
2cO
3in solution, survey OD value at 600nm place with spectrophotometer, with Na
2cO
3solution is made blank.Weigh and spleen re-computation phagocytic index according to the weight of animals, liver.
2.6.2, Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment: animal gives sample after 30 days continuously, every Mus lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30min, cervical vertebra dislocation is put to death, be fixed on Mus plate, cut off abdominal skin, injecting normal saline 2ml, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml, divide and drip on 2 slides, 37 DEG C of wet 30min of incubating of incubator, use normal saline rinsing, dry, fix with 1:1 acetone methanol solution, 4%Giemsa-phosphate buffer dyeing 3min, dry with distilled water rinsing again, with oily mirror microscopy, calculate phagocytic percentage (%) and phagocytic index.
2.7, NK cytoactive detection: animal is given sample after 30 days continuously, and the dislocation of animal cervical vertebra is put to death, and gets spleen, makes splenocyte suspension (effector lymphocyte), get go down to posterity after 24h YAC-1 cell add RPMI1640 complete culture solution, adjusting cell concentration is 4 × 10
5individual/ml(target cell), get the each 100 μ l(effect targets of target cell and effector lymphocyte than 50:1), add U-shaped 96 orifice plates, target cell Spontaneous release hole adds target cell and the each 100 μ l of culture fluid, maximum release aperture adds target cell and the each 100 μ l of 1%NP40, above-mentioned every three multiple holes of all establishing, in 37 DEG C, 5%CO2 incubator, cultivate 4h, draw at the bottom of supernatant 100 μ l horizontalizations in 96 orifice plates in every hole, add LDH substrate liquid 100 μ l simultaneously, reaction 3min, every hole adds the HCl 30 μ l of 1mol/l to stop, and measures OD value by microplate reader at 490nm place.
2.8, statistics: experimental result is carried out statistical analysis with SPSS15.0 software, and each dosage is respectively at matched group comparison.
3. result
Any two aspect result positives aspect four of cellular immune functions, humoral immune function, monocytes/macrophages function, NK cytoactive, can judge that Herba Dendrobii granule of the present invention has enhancing immunity function.The wherein experiment of two in cellular immune function test item (being mouse spleen lymphocyte transformation experiment and delayed allergy experiment) result is all positive, or two Dose Results of any experiment are positive, can judge the cellular immune function assay result positive.Result is all positive, or two Dose Results of any experiment are positive, can judge the humoral immune function measurement result positive in two experiments (being that antibody-producting cell detects and serum hemolysin is measured) in humoral immune function mensuration project.Two experiments (being that mice carbon is cleaned up experiment and Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment) result in monocytes/macrophages functional examination project is all positive, or two Dose Results of any experiment are positive, can judge the monocytes/macrophages function result positive.More than one dosage group result of NK cytoactive detection experiment is positive, can judge the NK cytoactive result positive.
Table 1: immune one group (NK cytoactive, splenocyte transformation experiment) result
(n=10)
Note: * Analysis of variance, compared with matched group, p<0.01
From table 1, the mice of the each dosage group of Herba Dendrobii granule of the present invention is initial, in experiment, test last body weight and weightening finish compared with matched group, difference that there are no significant; NK cytoactive data transaction
after carry out variance analysis, the NK cytoactive of each dosage group compared with matched group, difference that there are no significant; In the mouse spleen lymphocyte transformation experiment of ConA induction, the OD difference of sample 3.0g/kg dosage group, compared with matched group, has significant difference (p<0.01).
Table 2: immune two groups (DTH, spleen index and thymus index experiment) results
(n=10)
Note: * Analysis of variance, compared with matched group, p<0.01
From table 2, the mice of the each dosage group of Herba Dendrobii granule of the present invention is initial, in experiment, test last body weight and weightening finish compared with matched group, difference that there are no significant; The mouse spleen index of the each dosage group of sample and thymus index respectively compared with matched group, difference that there are no significant; In DTH experiment, the OD difference of sample 3.0g/kg dosage group, compared with matched group, has significant difference (p<0.01).
Immune three groups (hemolysis plaque and hemolysin experiment) results of table 3
(n=10)
From table 3, the mice of the each dosage group of Herba Dendrobii granule of the present invention is initial, in experiment, test last body weight and weightening finish compared with matched group, difference that there are no significant; Antibody-producting cell detect in, the plaque number of the each dosage group of Herba Dendrobii granule of the present invention compared with matched group, difference that there are no significant; In serum hemolysin experiment, the plaque number of the each dosage group of Herba Dendrobii granule of the present invention compared with matched group, difference that there are no significant.
Table 4: immune four groups (carbon is cleaned up experiment) results
(n=10)
Note: # Analysis of variance, compared with matched group, p<0.05; * Analysis of variance, compared with matched group, p<0.01
From table 4, the mice of the each dosage group of Herba Dendrobii granule of the present invention is initial, in experiment, test last body weight and weightening finish compared with matched group, difference that there are no significant; Carbon is cleaned up in experiment, the phagocytic index of sample 1.0g/kg and 3.0g/kg dosage group is compared with matched group, variant (p<0.05), the phagocytic index of 3.0g/kg dosage group, compared with matched group, has significant difference (p<0.01).
Table 5: immune five groups (engulfing experiment) results
(n=10)
From table 5, the mice of the each dosage group of Herba Dendrobii granule of the present invention is initial, in experiment, test last body weight and weightening finish compared with matched group, difference that there are no significant; Turnover of Mouse Peritoneal Macrophages is engulfed in chicken red blood cell experiment, engulfs a point rate data transaction
obtain by variance analysis, the phagocytic percentage of the each dosage group of Herba Dendrobii granule of the present invention compared with matched group, difference that there are no significant; The phagocytic index of the each dosage group of sample compared with matched group, difference that there are no significant.
4. conclusion: under this experimental condition, the cellular immune function positive, the monocytes/macrophages function positive, but humoral immune function and NK cytoactive result are negative, judge according to the result of " increase immunity function " in " health food inspection and assessment technique specification " (version in 2003), think that its mouse oral gave Herba Dendrobii granule of the present invention after 30 days, had enhancing immunity function to mice.
Detailed description of the invention
Applicant finds under study for action: healthy energy is that immunity is to maintain the physiological function of body and have the ability of resisting exopathogen."Nei Jing" is said " healthy energy deposit in, heresy can not be done ".All kinds of diseases and ailments are all because of the positive deficiency of vital energy, therefore ancients say " deficiency syndrome should be treated by tonifying method ".We are the deficiency syndrome for positive QI-insufficiency by tonify deficiency rule for the treatment of.This card is mainly because of due to the five internal organs deficiency of vital QI.Insufficiency of primordial QI, YIN fluid deficiency, lung spleen is become estranged, and has spiritlessness and weakness, tired, the dizzy insomnia of the tired body of body, the lazy speech of breathing hard, dry pharynx dryness of the tongue, tired thist with desire to drink, many discomforts such as afternoon flushed cheeks.Side is main with Herba Dendrobii, Radix Ginseng, is aided with Rhizoma Dioscoreae, Poria, Semen Coicis, Fructus Lycii.Herba Dendrobii tonifying-Yin and nourishing-stomach, clearing away heat and promoting production of body fluid, improving eyesight is kept fit; Radix Ginseng strongly invigorating primordial QI, invigorating the spleen to benefit the lung, promoting the production of body fluid and inducing sedation of the mind; Rhizoma Dioscoreae strengthening the spleen and stomach, promoting the production of body fluid and benefiting QI, the power of enhancing the kidney invigorating gas; Poria, Semen Coicis strengthening the spleen mind calming, promoting diuresis to eliminate damp pathogen, the gas of devote every effort to help Radix Ginseng tonifying the lung spleen, the change source of real QI and blood life.Fructus Lycii invigorating the liver and kidney, benefiting essence-blood, improving eyesight is moisturized.Full side is mutually auxiliary is use, and all medicines share the effect of playing altogether strengthening the body resistance, nourishing the liver and kidney, the foster lung of spleen reinforcing.
Applicant, on the basis of foregoing invention principle, through experiment in large quantities, has obtained formula and the preparation technology of Herba Dendrobii granule of the present invention.
Herba Dendrobii granule of the present invention, mainly formed by Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis, wherein, the weight proportion of described Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis is: 15～28:30～42:7～18:3～13:15～28:15～28.
Preparation technology of the present invention, comprises the steps:
1, according to the weight proportion of Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis be: draw materials in 15～28:30～42:7～18:3～13:15～28:15～28.
2, Herba Dendrobii is put in multi-function extractor, added 20 times of water gagings and soak 4h, decoct 1.5h, filter to obtain the first filtering residue and the first filtrate;
3, the first filtering residue, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae and Semen Coicis are put in multi-function extractor, added 12 times of water gagings and decoct 1h, filter to obtain the second filtering residue and the second filtrate;
4, the second filtering residue is put in multi-function extractor, added 10 times of water gagings and decoct 1h, filter to obtain the 3rd filtering residue and the 3rd filtrate;
5, merge the first filtrate, the second filtrate and the 3rd filtrate, after filtering with flame filter press, filtrate is placed in concentrating under reduced pressure tank; Under 55-65 DEG C, vacuum 0.07-0.09MPa, being evaporated to relative density is 1.15-1.20(60 DEG C of survey), obtain concentrated extract;
6, in concentrated extract, adding quality is concentrated extract 1.5-2.5 beta-schardinger dextrin-doubly, is spray dried to extract powder.
7, dry granulation, the granulate that sieves obtains Herba Dendrobii granule.
In the present invention Six-element drug matching rationally proper, clear efficacy, have no side effect, the effect of tool enhancing immunity, alleviating physical fatigue, adopt granule dosage form, taking beta-schardinger dextrin-as excipient, granule has the features such as bioavailability is high, the good mouthfeel of dissolubility is good, easy to carry, and whole technique relates to technology and does not produce the waste material of environmental pollution, not producing technique waste water, is a kind of economic environment-friendly type technique, is applicable to enterprise and produces and apply, good product quality, economic worth is high.
Process conditions are groped as follows:
Embodiment 1: the investigation of extraction time to Herba Dendrobii extraction ratio
The Herba Dendrobii of getting same amount carries out the investigation experiment of extraction time, and fixing solid-liquid ratio is 1:40(g/ml), 2 hours extraction times, 3 hours, 3.5 hours, 4 hours, carry out the mensuration of crude polysaccharides content.Result is as follows:
Table 6: the impact of different extraction times on Herba Dendrobii coarse polysaccharide extractive rate
Above experimental result shows, under the identical extraction water yield, the extraction ratio of crude polysaccharides improves along with the increase of extraction time also has correspondingly, extracts and has increase significantly than the extraction ratio of 3 hours, 2 hours crude polysaccharides in 3.5 hours; The extraction ratio of comparison 4 hours and 3.5 hours crude polysaccharides, extraction ratio is without significant difference.Therefore,, from the factor consideration such as energy consumption and man-hour, selected extraction time is 3.5 hours.
Embodiment 2: extract the water yield to Herba Dendrobii extraction ratio investigation
The Herba Dendrobii of getting same amount extracts the investigation experiment of the water yield, and fixing solid-liquid ratio is 1:20; 1:30,1:40,1:50, extraction time is 3.5 hours, carries out the mensuration of crude polysaccharides content, experimental result is as follows:
Table: 7: the impact of the different extraction water yields on Herba Dendrobii coarse polysaccharide extractive rate
| Experiment number | Extract the water yield (g/ml) | Coarse polysaccharide extractive rate (%) (pressing medical material calculates) |
| 1 | 20 | 46.5 |
| 2 | 30 | 58.1 |
| 3 | 40 | 67.4 |
| 4 | 50 | 69.5 |
Because the extraction of polysaccharide is the process that solid solute dissolves gradually, therefore along with the increase of solid-to-liquid ratio, Dendrobium officinale polysaccharide extracts the trend that yield is growth.Especially before solid-liquid ratio 1:40, the extraction yield rapid development of polysaccharide, but after 1:40, polysaccharide yield increases and is slow growth trend with solid-liquid ratio, consider that quantity of solvent increases to the follow-up concentrated consumption that brings time and energy, from the viewpoint of saving time, energy-conservation etc., the solid-liquid ratio of 1:4O carries out the extraction of Herba Dendrobii, can obtain reasonable extraction effect.
Consider each side factor, the extraction conditions of Herba Dendrobii is decided to be: Herba Dendrobii adds 20 times of water heating extraction 1 time, 1.5 hours, leach medicinal liquid, its medicinal residues again with prescription in other a few taste medical materials together with extract again 2 times, each 1 hour.By this production technology, Herba Dendrobii is obtained good extraction effect, and coarse polysaccharide extractive rate is high.
Embodiment 3: the Study on extraction of the medical materials such as Radix Ginseng, Poria, Fructus Lycii
Herba Dendrobii list is decocted 1 time, leaches in medicinal residues after medicinal liquid and prescription the medical materials such as Radix Ginseng and decocts altogether.In order to find out best extraction conditions, taking functional component total saponins (g) as index, selecting four factor three water-glasses is L
9(3
4) table, amount of water (A), decocting time (B), three factors of decoction number of times (C) are carried out to orthogonal test, each factor is all defined as three levels, in table 3.Experimental establishment, experimental result and date processing are in table 4.
Table 8: experimental factor water-glass
Experimental technique and result:
Take 9 parts of medical materials of identical weight by formula proportion, experimental establishment according to table 5 decocts respectively, the medicinal liquid of gained merges, filter, be concentrated into certain volume, method under the method for inspection [mensuration of the total saponins] item of employing functional component, carries out assay to the total saponins in each tested number sample respectively.The results are shown in Table 4.
Table 9:L9(34) experimental establishment, experimental result and date processing table
Variance analysis is as follows:
The orthogonal experiments of table 9 is carried out to variance analysis, the results are shown in Table 10
Table 10: analysis of variance table
Note: F
1-0.01(2,2)=99.0 F
1-0.05(2,2)=19.0 F
1-0.10(2,2)=9.0 interpretation of result is as follows:
I j, II j, IIIj value in his-and-hers watches 4 are carried out intuitive analysis, and relatively R value is known, influence degree C > B > A; But on the impact of experimental result how the level of each factor changes actually, utilizes variance analysis further to investigate.The results of analysis of variance of table 5 shows: F
a< F
1-0.10(2,2), the level of factor A changes experimental result impact little; F
b> F
1-0.10(2,2), the level of factor B changes has impact greatly to experimental result; F
1-0.01(2,2) > F
c> F
1-0.05(2,2), the level variation of factor C has a significant impact experimental result.From time-saving energy-saving, reduce costs equal angles and consider, extraction process scheme is decided to be: medical material adds 12 times of water gagings (consider and have the water yield of 2 times of left and right to be inhaled profit by medical material while extraction for the first time) for the first time, extracts 1 hour; Add for the second time 10 times of water gagings, extract 1 hour, obtained more satisfactory extraction effect by this technique active ingredient total saponins.
Embodiment 4: orthogonal processing confirmatory experiment
The 3 parts of medical materials of medical material that take identical weight by formula proportion, carry out extraction process confirmatory experiment.Extraction conditions: medical material adds 12 times of water gagings for the first time, extracts 1 hour; Add for the second time 10 times of water gagings, extract 1 hour, filter merging extracted twice liquid and be concentrated into certain volume; Carry out three parts of experiments by above condition is parallel.By the functional component assay method specifying in target level of product quality, measure the content of its functional component total saponins, and investigate the feasibility of extraction process, the results are shown in following table.
Table 6: the testing result of three parts of verification samples
| Sample | Total saponins (in ginsenoside Re) mg |
| 1 | 412 |
| 2 | 427 |
| 3 | 418 |
As seen from the above table, in three batches of lab scale samples, the content of the total saponins of effective component is basically identical, and extraction ratio approaches, and says that the extraction process of this product is stablized, feasible.
Embodiment 5
Get 37.5kg Herba Dendrobii, put in extraction pot, add 20 times of amount warm water soaking 4h, decoct 1.5h, filter to obtain filtering residue 1 filtrate 1;
(2) get filtering residue 1, add Poria 62.5kg, Radix Ginseng 17.5kg, Fructus Lycii 2.5kg, Rhizoma Dioscoreae 18.75kg, Semen Coicis 18.75kg, put in extraction pot, add 12 times of water gagings and decoct 1h, filter to obtain filtering residue 2 and filtrate 2, get filtering residue 2 and add 10 times of water gagings decoction 1h, filter to obtain filtering residue 3 and filtrate 3;
(3) merging filtrate 1, filtrate 2 and filtrate 3, after filtering with flame filter press, filtrate is placed in vacuum concentration pot, vacuum concentration (temperature 50-70 DEG C, vacuum 0.07-0.09MPa) to relative density is 1.15-1.20(60 DEG C), obtain thick extractum 33.75kg;
(4) take 75kg beta-schardinger dextrin-, sneak in thick extractum, limit edged stirs makes soft material;
(5) soft material is crossed to 12 mesh sieves and granulated, the wet granular of making is put (temperature 65-75 DEG C) in baking oven, dry to pellet moisture≤5.0%, and, obtain granule 148kg.
This technique is studied prescription composition, for the feature of the each medicine of prescription composition, carries out technological design, and has selected optimum extraction process.
Drying process: this process choice spray drying method, extractum adds formula ratio adjuvant, directly sprays dry.Spray-dired feature: 1. rate of drying is fast, moisture flash evapn, is applicable to thermal sensitivity medicine; 2. dry rear products obtained therefrom color and luster is good, and floury texture is loose, and solubility property is good, improves dissolution rate; 3. change drying condition easy; 4. product is not contaminated, and dry run is carried out under air-tight state; 5. automaticity is high, can produce continuously, enhances productivity.
Granulating process: this process choice dry granulation, is directly prepared into extract powder dry granule.Dry granulation has following characteristics: 1. do not need to add other adhesive, and finished particle epigranular, outward appearance and good fluidity, disintegration is easy to control; 2. particularly applicable for the material of the wet easy decomposition failure of heat chance or caking; 3. with respect to other granulations, can be directly by Chinese medicinal powder granulations such as spray powders, eliminate water and solution and carried out the drug degradation that wet granulation causes, save the energy cost of wet granular after drying, without or use less intermediate and additive, greatly reduce supplementary product consumption, shorten the manufacturing cycle, can be specially adapted to heat to heat sensitive material and meet the material of wet easy decomposition failure or caking and carry out pelletize.After granulation, can increase product mobility and density.
Above-described embodiment is used for the present invention that explains, instead of limits the invention, and in the protection domain of spirit of the present invention and claim, any amendment and change that the present invention is made, all fall into protection scope of the present invention.
Claims (1)
1. the Herba Dendrobii granule of an enhancing immunity, it is characterized in that, it is made up of Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis, wherein, the weight proportion of described Herba Dendrobii, Poria, Radix Ginseng, Fructus Lycii, Rhizoma Dioscoreae, Semen Coicis is: 15～28:30～42:7～18:3～13:15～28:15～28.
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| CN105725209A (en) * | 2016-01-28 | 2016-07-06 | 广西大学 | Dendrobium officinale granules and preparation method thereof |
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