CN105816258A - Artificial insemination method of breeder cocks - Google Patents

Artificial insemination method of breeder cocks Download PDF

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Publication number
CN105816258A
CN105816258A CN201610140554.0A CN201610140554A CN105816258A CN 105816258 A CN105816258 A CN 105816258A CN 201610140554 A CN201610140554 A CN 201610140554A CN 105816258 A CN105816258 A CN 105816258A
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China
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semen
artificial insemination
cock
kind
seminal fluid
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CN201610140554.0A
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Chinese (zh)
Inventor
马子玉
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永胜玉水农特开发有限责任公司
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Priority to CN201610140554.0A priority Critical patent/CN105816258A/en
Publication of CN105816258A publication Critical patent/CN105816258A/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination

Abstract

The invention provides an artificial insemination method of breeder cocks. The method comprises the following steps: 1) artificially feeding breeder cocks before semen collection, namely selecting 55-week-old artificial insemination breeder cocks, adding feed additives to the conventional feed according to a ratio of 1.5-2.0wt%, feeding the breeder cocks until the breeder cocks are at the age of 68 weeks or the production cycle is ended according to a feeding procedure of continuously feeding for 7 days and later stopping for 7 days; 2) collecting semens; 3) diluting the semens, namely using semen diluents of which the volume is 2-4 times of that of the semen and slowly adding to semen collecting test tubes along the tube walls of the semen collecting test tubes, and then gently turning the semen collecting test tubes and repeating for 3-4 times; 4) carrying out artificial insemination, namely turning the anus of hens by anus turning members, transferring the diluted semens to deferent ducts by inseminators, and then gently inserting the deferent ducts into fallopian tubes of the hens by the inseminators and conveying the semens in the deferent ducts to conveying pipes, so as to realize the artificial insemination. The artificial insemination method disclosed by the invention is high in semen viability and insemination rate.

Description

Plant the artificial insemination method of chicken

Technical field

The invention belongs to poultry breeding technologies field, be specifically related to the artificial insemination method of a kind chicken.

Background technology

Along with the development of modernization aviculture, scale and the intensive degree of poultry farming are more and more higher, family The artificial insemination of fowl is more and more important.The main advantages of chicken technology of artificial insemination is to accelerate improved variety process, Improve the utilization rate of outstanding kind of chicken, reduce the stock cock number of animals raised two and save feeding cost, improve insemination rate and increase Add the chickling number that every hen provides, utilize Disease epizootic etc..

The technology of artificial insemination planting chicken is requisite means in modern breeding and production, due to poultry sperm Density is big and volume of semen little, after dilution, semen deposition can utilize the seminal fluid of outstanding stock cock, more to greatest extent Feeding cost can be saved, can increase economic efficiency.Along with kind of chicken coop supports popularization and the local varieties conservation of technology And the needs of breeding, the technology of artificial insemination of chicken is used widely, and has the most become during poultry produces The conventional application technology of item, but traditional artificial insemination method often causes the cross infection of fowl disease, threatens Hen is healthy, affects hen egg production, rate of fertilization.

Owing to stock cock of raising in cages is usually single cage monoculture, the intensity of utilization is relatively raised scattered and is greatly increased, do not has in addition There is special industry feeding standard, therefore plant is relatively big to its blindness raised, and often leads to cock and is not having There is sexual function senilism before reaching to use the normal time limit, directly affects kind of chicken and lay eggs the rate of fertilization in later stage, right Zhong Ji plant causes greater loss.

Summary of the invention

For solving the problem that motility of sperm is low and insemination rate is low of existing kind of chicken, the present invention proposes a kind chicken Artificial insemination method, this artificial insemination method motility of sperm is high and insemination rate is high.

The technical scheme is that and be achieved in that:

An artificial insemination method for kind chicken, comprises the following steps:

1) plant with the artificial feeding before cock semen collection: choose the artificial insemination stock cock of 55 week old, to routine In feedstuff, the ratio according to 1.5-2.0wt% adds feed additive, stops the raising program of 7 days after feeding 7 days continuously Feed to kind of chicken 68 week old or production cycle and terminate;

2) semen collection: by step 1) feed the kind that obtains and carry out semen collection training with cock, and at 15-20 prefecundation It carries out quality testing and Pullorum Disease detection to planting with the seminal fluid of cock, leaves and takes satisfactory kind and uses cock, And the kind anal feather of cock is wiped out, before semen deposition, take collection essence cup collect kind of the seminal fluid using cock;

3) dilution seminal fluid: use the semen diluent of 2-4 times of seminal fluid volume slowly to add along collection essence test tube tube wall Enter in collection essence test tube, rotate collection essence test tube the most gently, repeat 3-4 time;

4) artificial insemination: turn over anus person and hen is turned over anus, the seminal fluid after dilution is transferred to semen deposition by semen deposition person In pipe, carry in then deferent duct is inserted the fallopian tube of hen by semen deposition person gently and by the seminal fluid in deferent duct In conveying pipe;

Wherein, step 1) in feed additive be made up of with nutritional feed additive compound Chinese herbal medicine additive, Described compound Chinese herbal medicine additive includes that following raw material is made: Herba Dendrobii 8-16wt%, Rhizoma Dioscoreae 6-13wt%, Radix Rehmanniae Preparata Yellow 10-15wt%, Fructus Lycii 3-6wt%, Semen Plantaginis 5-8wt%, 7-10wt% Radix Ophiopogonis, Radix Codonopsis 10-16wt%, Radix Angelicae Sinensis 7-10wt%, Radix Glycyrrhizae 5-8wt% and Cordyceps 8-12wt%;Described nutritional feed additive includes following Raw material is made: Aloe dry powder 25-35wt%, Radix et Caulis Opuntiae Dillenii dry powder 20-25wt%, powder of Folium Mori 25-35wt%, cunning Powder 5-8wt%, Mel 5-8wt% and compound vitamin 2-4wt%.

Further, calculating in parts by weight, described semen diluent is settled to distilled water by following components 1000 milliliters formulated, and described component is monosaccharide 10-15 part, antibiotics 0.8-1.2 part, vitamin 6-9 Part, disodium hydrogen phosphate 2-2.5 part, sodium dihydrogen phosphate 0.2-0.4 part and glutamic acid 0.4-0.6 part.

Beneficial effects of the present invention:

1, the present invention starts with by cock body constitution and kind character from conditioning kind, utilizes Chinese herbal medicine and nutrition " multi-source Multiple-effect " theory, develop conditioning artificial insemination stock cock performance feed additive, prepare this feedstuff Raw material is easy to get, and using method is simple, and input cost is relatively low, has good using value.Use the present invention Feed additive, the average volume of semen of stock cock improve 23%;(eupyrene sperm accounts for total sperm ratio to motility of sperm Example) improve 25-30%;Sperm concentration improves 20-25%;The senilism rate of stock cock reduces 46-48%.

2, can be the lowest even dead due to sperm time the longest insemination rate in hen vagina, insemination rate is often Relatively low, on the one hand hen laying rate receives impact, on the other hand, in order to gather more seminal fluid, needs to support Grow substantial amounts of stock cock to meet artificial insemination needs, increase aquaculture cost.The semen diluent of the present invention is Sperm provides nutrient substance, extends Sperm survival time, strengthens the activity of sperm, improves insemination rate.

Detailed description of the invention

Embodiment 1

1) plant with the artificial feeding before cock semen collection: choose the artificial insemination stock cock of 55 week old, to routine In feedstuff, the ratio according to 1.5wt% adds feed additive, and the raising program stopped 7 days after feeding 7 days continuously is raised It is fed to kind of chicken 68 week old;

2) semen collection: by step 1) feed the kind that obtains and carry out semen collection training with cock, and 15 days prefecundations Carry out quality testing and Pullorum Disease detection to planting with the seminal fluid of cock, leave and take satisfactory kind and use cock, In the environment of peace and quiet, the feather around stock cock cloaca is cut off, a diameter of 4-5cm of exposed area, this Cause the pollution of seminal fluid in sample is possible to prevent chicken manure, feather bits or dust to drop into semen collection cup, thus cause The defect that rate of fertilization is low, then collector catches stock cock, and before Caudad, stock cock backward, is fixed by head In the oxter of collector, two lower limbs are nature stances, and collector is clipped in collection essence cup in the right hand and the third finger Between, outwardly with the back of the hand in the same direction, left hand thumb refers to separate rim of a cup with four, is attached in the wing of both sides, stock cock back Side, rearwardly massage slip 2-3 time is gently held up in region, and stock cock so can be made to form normal sexual reflex, profit In producing essence, hand thumb refers to separate the abdominal part of massage phalanx lower edge the most gently, stock cock with four simultaneously Mating device when turning up, left-handed forefinger and thumb are mentioned mating device and extrude mating device, and right hand reversing is used Collection essence cup accesses cloaca seminal fluid, immediately in shift-in collection essence test tube;

3) dilution seminal fluid: use the semen diluent of 3 times of seminal fluid volumes slowly to add along collection essence test tube tube wall Enter in collection essence test tube, rotate collection essence test tube the most gently, be repeated 3 times;

4) artificial insemination: turn over anus person and hen is turned over anus, the seminal fluid after dilution is transferred to semen deposition by semen deposition person In pipe, carry in then deferent duct is inserted the fallopian tube of hen by semen deposition person gently and by the seminal fluid in deferent duct In conveying pipe;

Wherein, step 1) in feed additive be made up of with nutritional feed additive compound Chinese herbal medicine additive, Described compound Chinese herbal medicine additive includes that following raw material is made: Herba Dendrobii 8wt%, Rhizoma Dioscoreae 13wt%, Radix Rehmanniae Preparata 15wt%, Fructus Lycii 3wt%, Semen Plantaginis 8wt%, 10wt% Radix Ophiopogonis, Radix Codonopsis 16wt%, Radix Angelicae Sinensis 7wt%, sweet Grass 8wt% and Cordyceps 12wt%;Described nutritional feed additive includes that following raw material is made: Aloe dry powder 30wt%, Radix et Caulis Opuntiae Dillenii dry powder 25wt%, powder of Folium Mori 30wt%, smoothers 5wt%, Mel 8wt% and compound dimension Raw element 2wt%.Semen diluent by following components and distilled water be settled to 1000 milliliters formulated, described Component is monosaccharide 10 parts, 1.2 parts of antibiotics, vitamin 9 parts, disodium hydrogen phosphate 2.5 parts, biphosphate 0.4 part of sodium and 0.4 part of glutamic acid.

Embodiment 2

1) plant with the artificial feeding before cock semen collection: choose the artificial insemination stock cock of 55 week old, to routine In feedstuff, the ratio according to 2.0wt% adds feed additive, and the raising program stopped 7 days after feeding 7 days continuously is raised It is fed to kind of chicken 68 week old or the production cycle terminates;

2) semen collection: by step 1) feed the kind that obtains and carry out semen collection training with cock, and 18 days prefecundations Carry out quality testing and Pullorum Disease detection to planting with the seminal fluid of cock, leave and take satisfactory kind and use cock, and The kind anal feather of cock is wiped out, before semen deposition, takes collection essence cup collect kind of the seminal fluid using cock;

3) dilution seminal fluid: use the semen diluent of 2 times of seminal fluid volumes slowly to add along collection essence test tube tube wall Enter in collection essence test tube, rotate collection essence test tube the most gently, be repeated 4 times;

4) artificial insemination: turn over anus person and hen is turned over anus, the seminal fluid after dilution is transferred to semen deposition by semen deposition person In pipe, carry in then deferent duct is inserted the fallopian tube of hen by semen deposition person gently and by the seminal fluid in deferent duct In conveying pipe;

Wherein, step 1) in feed additive be made up of with nutritional feed additive compound Chinese herbal medicine additive, Described compound Chinese herbal medicine additive includes that following raw material is made: Herba Dendrobii 16wt%, Rhizoma Dioscoreae 11wt%, Radix Rehmanniae Preparata 10wt%, Fructus Lycii 6wt%, Semen Plantaginis 8wt%, 10wt% Radix Ophiopogonis, Radix Codonopsis 13wt%, Radix Angelicae Sinensis 10wt%, Radix Glycyrrhizae 8wt% and Cordyceps 8wt%;Described nutritional feed additive includes that following raw material is made: Aloe dry powder 25wt%, Radix et Caulis Opuntiae Dillenii dry powder 25wt%, powder of Folium Mori 32wt%, smoothers 7wt%, Mel 8wt% and compound dimension Raw element 3wt%.Calculating in parts by weight, described semen diluent is settled to distilled water by following components 1000 milliliters formulated, and described component is monosaccharide 15 parts, 1 part of antibiotics, vitamin 6 parts, phosphoric acid hydrogen Disodium 2 parts, sodium dihydrogen phosphate 0.3 part and 0.5 part of glutamic acid.

Embodiment 3

1) plant with the artificial feeding before cock semen collection: choose the artificial insemination stock cock of 55 week old, to routine In feedstuff, the ratio according to 1.8wt% adds feed additive, and the raising program stopped 7 days after feeding 7 days continuously is raised It is fed to kind of chicken 68 week old or the production cycle terminates;

2) semen collection: by step 1) feed the kind that obtains and carry out semen collection training with cock, and 20 days prefecundations Carry out quality testing and Pullorum Disease detection to planting with the seminal fluid of cock, leave and take satisfactory kind and use cock, In the environment of peace and quiet, the feather around stock cock cloaca is cut off, a diameter of 4-5cm of exposed area, this Cause the pollution of seminal fluid in sample is possible to prevent chicken manure, feather bits or dust to drop into semen collection cup, thus cause The defect that rate of fertilization is low, then collector catches stock cock, and before Caudad, stock cock backward, is fixed by head In the oxter of collector, two lower limbs are nature stances, and collector is clipped in collection essence cup in the right hand and the third finger Between, outwardly with the back of the hand in the same direction, left hand thumb refers to separate rim of a cup with four, is attached in the wing of both sides, stock cock back Side, rearwardly massage slip 2-3 time is gently held up in region, and stock cock so can be made to form normal sexual reflex, profit In producing essence, hand thumb refers to separate the abdominal part of massage phalanx lower edge the most gently, stock cock with four simultaneously Mating device when turning up, left-handed forefinger and thumb are mentioned mating device and extrude mating device, and right hand reversing is used Collection essence cup accesses cloaca seminal fluid, immediately in shift-in collection essence test tube;

3) dilution seminal fluid: use the semen diluent of 4 times of seminal fluid volumes slowly to add along collection essence test tube tube wall Enter in collection essence test tube, rotate collection essence test tube the most gently, be repeated 4 times;

4) artificial insemination: turn over anus person and hen is turned over anus, the seminal fluid after dilution is transferred to semen deposition by semen deposition person In pipe, carry in then deferent duct is inserted the fallopian tube of hen by semen deposition person gently and by the seminal fluid in deferent duct In conveying pipe;

Wherein, step 1) in feed additive be made up of with nutritional feed additive compound Chinese herbal medicine additive, Described compound Chinese herbal medicine additive includes that following raw material is made: Herba Dendrobii 8wt%, Rhizoma Dioscoreae 13wt%, Radix Rehmanniae Preparata 15wt%, Fructus Lycii 5wt%, Semen Plantaginis 7wt%, 9wt% Radix Ophiopogonis, Radix Codonopsis 15wt%, Radix Angelicae Sinensis 9wt%, sweet Grass 7wt% and Cordyceps 12wt%;Described nutritional feed additive includes that following raw material is made: Aloe dry powder 35wt%, Radix et Caulis Opuntiae Dillenii dry powder 20wt%, powder of Folium Mori 25wt%, smoothers 8wt%, Mel 8wt% and compound dimension Raw element 24wt%.Calculating in parts by weight, described semen diluent is settled to distilled water by following components 1000 milliliters formulated, and described component is monosaccharide 12 parts, 0.8 part of antibiotics, vitamin 8 parts, phosphoric acid Disodium hydrogen 2.2 parts, sodium dihydrogen phosphate 0.3 part and 0.5 part of glutamic acid.

The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this Within bright spirit and principle, any modification, equivalent substitution and improvement etc. made, should be included in this Within bright protection domain.

Claims (2)

1. the artificial insemination method of a kind chicken, it is characterised in that comprise the following steps:
1) plant with the artificial feeding before cock semen collection: choose the artificial insemination stock cock of 55 week old, to routine In feedstuff, the ratio according to 1.5-2.0wt% adds feed additive, stops the raising program of 7 days after feeding 7 days continuously Feed to kind of chicken 68 week old or production cycle and terminate;
2) semen collection: by step 1) feed the kind that obtains and carry out semen collection training with cock, and at 15-20 prefecundation It carries out quality testing and Pullorum Disease detection to planting with the seminal fluid of cock, leaves and takes satisfactory kind and uses cock, And the kind anal feather of cock is wiped out, before semen deposition, take collection essence cup collect kind of the seminal fluid using cock;
3) dilution seminal fluid: use the semen diluent of 2-4 times of seminal fluid volume slowly to add along collection essence test tube tube wall Enter in collection essence test tube, rotate collection essence test tube the most gently, repeat 3-4 time;
4) artificial insemination: turn over anus person and hen is turned over anus, the seminal fluid after dilution is transferred to semen deposition by semen deposition person In pipe, carry in then deferent duct is inserted the fallopian tube of hen by semen deposition person gently and by the seminal fluid in deferent duct In conveying pipe;
Wherein, step 1) in feed additive be made up of with nutritional feed additive compound Chinese herbal medicine additive, Described compound Chinese herbal medicine additive includes that following raw material is made: Herba Dendrobii 8-16wt%, Rhizoma Dioscoreae 6-13wt%, Radix Rehmanniae Preparata Yellow 10-15wt%, Fructus Lycii 3-6wt%, Semen Plantaginis 5-8wt%, 7-10wt% Radix Ophiopogonis, Radix Codonopsis 10-16wt%, Radix Angelicae Sinensis 7-10wt%, Radix Glycyrrhizae 5-8wt% and Cordyceps 8-12wt%;Described nutritional feed additive includes following Raw material is made: Aloe dry powder 25-35wt%, Radix et Caulis Opuntiae Dillenii dry powder 20-25wt%, powder of Folium Mori 25-35wt%, cunning Powder 5-8wt%, Mel 5-8wt% and compound vitamin 2-4wt%.
The artificial insemination method of the most according to claim a kind of chicken, it is characterised in that according to weight portion Number calculate, described semen diluent by following components and distilled water be settled to 1000 milliliters formulated, described Component be monosaccharide 10-15 part, antibiotics 0.8-1.2 part, vitamin 6-9 part, disodium hydrogen phosphate 2-2.5 part, Sodium dihydrogen phosphate 0.2-0.4 part and glutamic acid 0.4-0.6 part.
CN201610140554.0A 2016-03-11 2016-03-11 Artificial insemination method of breeder cocks CN105816258A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106386668A (en) * 2016-08-31 2017-02-15 李宏强 Efficient method for breeding and freely raising high-quality Jing Yuan chickens
CN106618790A (en) * 2016-10-19 2017-05-10 四川农业大学 Drake semen collecting method
CN107913118A (en) * 2017-12-19 2018-04-17 佛山科学技术学院 A kind of mule duck artificial insemination method

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CN101099751A (en) * 2006-07-04 2008-01-09 林文宏;康胜国际股份有限公司 Application of cordyceps militaris mycelium for promoting buck fertility
CN101623057A (en) * 2009-07-31 2010-01-13 山西天沐合生农牧科技开发有限公司 Chinese herbal medicine feed additive for stud rams
CN101816383A (en) * 2010-04-30 2010-09-01 西北农林科技大学 Special feed additive for conditioning breeding performance of artificially inseminated breeder cocks and applications thereof
CN102048959A (en) * 2011-01-14 2011-05-11 湖南龙石山铁皮石斛基地有限公司 Dendrobium candidum compound preparation capable of regulating function of human body in two-way manner and preparation method thereof
CN102885980A (en) * 2012-10-26 2013-01-23 李为彬 Traditional Chinese medicine (TCM) for treating azoospermia and preparation process thereof
CN102988827A (en) * 2012-11-21 2013-03-27 光明食品集团云南石斛生物科技开发有限公司 Dendrobium officinale granula
CN104509766A (en) * 2014-12-23 2015-04-15 金华寿仙谷药业有限公司 Dendrobium officinale beverage and preparation method thereof
CN104543596A (en) * 2015-02-02 2015-04-29 广东迈科特生物科技有限公司 Special composite vitamin premix feed preparation for breeding poultry as well as preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101099751A (en) * 2006-07-04 2008-01-09 林文宏;康胜国际股份有限公司 Application of cordyceps militaris mycelium for promoting buck fertility
CN101623057A (en) * 2009-07-31 2010-01-13 山西天沐合生农牧科技开发有限公司 Chinese herbal medicine feed additive for stud rams
CN101816383A (en) * 2010-04-30 2010-09-01 西北农林科技大学 Special feed additive for conditioning breeding performance of artificially inseminated breeder cocks and applications thereof
CN102048959A (en) * 2011-01-14 2011-05-11 湖南龙石山铁皮石斛基地有限公司 Dendrobium candidum compound preparation capable of regulating function of human body in two-way manner and preparation method thereof
CN102885980A (en) * 2012-10-26 2013-01-23 李为彬 Traditional Chinese medicine (TCM) for treating azoospermia and preparation process thereof
CN102988827A (en) * 2012-11-21 2013-03-27 光明食品集团云南石斛生物科技开发有限公司 Dendrobium officinale granula
CN104509766A (en) * 2014-12-23 2015-04-15 金华寿仙谷药业有限公司 Dendrobium officinale beverage and preparation method thereof
CN104543596A (en) * 2015-02-02 2015-04-29 广东迈科特生物科技有限公司 Special composite vitamin premix feed preparation for breeding poultry as well as preparation method and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106386668A (en) * 2016-08-31 2017-02-15 李宏强 Efficient method for breeding and freely raising high-quality Jing Yuan chickens
CN106618790A (en) * 2016-10-19 2017-05-10 四川农业大学 Drake semen collecting method
CN107913118A (en) * 2017-12-19 2018-04-17 佛山科学技术学院 A kind of mule duck artificial insemination method

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