CN108477377A - The preparation method of one seed oyster selenium-enriched protein powder - Google Patents
The preparation method of one seed oyster selenium-enriched protein powder Download PDFInfo
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- 241000237502 Ostreidae Species 0.000 title claims abstract description 190
- 235000020636 oyster Nutrition 0.000 title claims abstract description 188
- 239000011669 selenium Substances 0.000 title claims abstract description 131
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 123
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 122
- 239000000843 powder Substances 0.000 title claims abstract description 47
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 43
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 61
- 239000013535 sea water Substances 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 235000013372 meat Nutrition 0.000 claims abstract description 30
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 29
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims abstract description 25
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 25
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 25
- 235000014103 egg white Nutrition 0.000 claims abstract description 25
- 210000000969 egg white Anatomy 0.000 claims abstract description 25
- 238000003756 stirring Methods 0.000 claims abstract description 21
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 230000009849 deactivation Effects 0.000 claims abstract description 13
- 108091005804 Peptidases Proteins 0.000 claims abstract description 11
- 239000004365 Protease Substances 0.000 claims abstract description 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 210000001835 viscera Anatomy 0.000 claims abstract description 10
- 238000004108 freeze drying Methods 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims description 17
- 239000000693 micelle Substances 0.000 claims description 16
- 230000005672 electromagnetic field Effects 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 238000013019 agitation Methods 0.000 claims description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 238000010923 batch production Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 235000013575 mashed potatoes Nutrition 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- 235000013601 eggs Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Substances CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 1
- 238000012856 packing Methods 0.000 claims 1
- 235000021419 vinegar Nutrition 0.000 claims 1
- 239000000052 vinegar Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 9
- 102000008114 Selenoproteins Human genes 0.000 abstract description 6
- 108010074686 Selenoproteins Proteins 0.000 abstract description 6
- 239000011701 zinc Substances 0.000 description 8
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 6
- 229910052725 zinc Inorganic materials 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 3
- 206010039921 Selenium deficiency Diseases 0.000 description 3
- 230000001093 anti-cancer Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- OLYFZPNPLNSOMA-UHFFFAOYSA-N propan-2-one;2,2,2-trichloroacetic acid Chemical compound CC(C)=O.OC(=O)C(Cl)(Cl)Cl OLYFZPNPLNSOMA-UHFFFAOYSA-N 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102000011845 Iodide peroxidase Human genes 0.000 description 1
- 108010036012 Iodide peroxidase Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000001676 hydride generation atomic fluorescence spectroscopy Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses the preparation method of a seed oyster selenium-enriched protein powder, the preparation method includes：Oyster living is placed 10 15 days in the yeast selenium seawater containing 0.1 0.25% mass fraction；Oyster living is won into oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water by weight 10:10 50, it blends at a high speed, obtains oyster liquid and add protease weight ratio 100 into oyster liquid:0.5 1, it is that 30 50MPa stir 12 hours under conditions of temperature is 40 50 DEG C, Oyster Protein enzymolysis liquid is obtained by filtration in pressure；After Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying；The present invention prepares selenium rich of egg white powder using oyster as raw material, Zn content highest wherein in oyster, and Se content is relatively low, therefore the present invention adds yeast selenium culture 10 15 days by using oyster living, increases the content of selenoprotein in oyster.
Description
Technical field
The present invention relates to albumen powder preparing technical fields, and in particular to the preparation method of a seed oyster selenium-enriched protein powder.
Background technology
Oyster alias is called raw oyster, be in all foods containing zinc it is most abundant (per 100g oysters, do not include the weight of shell,
Aqueous 87.1%, 71.2mg containing zinc are rich in zinc albuminate, are good zinc supplementation food, zinc supplementation that can often eat oyster or zinc albuminate.
It is seashell for the entirety of Ostreidae (ostreae) animal oyster (ostreagigastnunb) and its nearly edge animal.In Asia
The torrid zone, the torrid zone are coastal all suitable for the cultivation of oyster, and China's distribution is very wide, and North gets the Yalu River, south is coastal all to produce oyster to Hainan Island.
Oyster, which is software, has shell, the animal of micropredation, salt-fresh water boundary to produce particularly fertile.
Selenium is micronutrient element needed by human, has anticancer, improves a variety of effects such as immunity, anti-aging, quilt
It is described as " Promethean fire ", " king of anticancer " etc..1973, WHO and international nutrition organization prescribed selenium were micro- necessary to humans and animals
Measure nutrient.Oneself confirms at present, and selenium is to constitute more than 30 kinds of selenoprotein in the mammalian body and selenoenzyme such as gluathione skin
The important composition ingredient of peroxidase, sulphur hydrogen reduction protease and Iodothyronine deiodinase etc., have it is anti-oxidant,
Anticancer improves the various biologicals functions such as immunity of organisms.The whole world has a countries and regions more than 40, hundred million people of about 5-10 to lack
Selenium.China has 2/3rds area to belong to Selenium deficiency area, wherein 30% is serious selenium deficiency area.Human body selenium is recommended by Chinese Soclety of Nutrition
Intake is 50-200 μ g/d, and China's city dweller's intake only 26-32 μ g/d, selenium intake are seriously relatively low.Utilize biology
Inorganic selenium is converted to Organic Selenium by conversion method, improves Selenium In Foods content, is the important channel for improving human body selemium nutrition.Pass through meals
Tonic fills selenium source, improves the immunity of the human body, and realizes that health of people is long-lived, is the livelihood issues that countries in the world are paid close attention to.It is rich
Selenium agricultural product are the high value agricultural product for having healthcare function, can be used for supply Se scientific.By Se-rich farm products supply Se scientific,
The selenium intake per capita of the selenium deficiency country such as Britain, Finland, Australia resident effectively improves.
Oyster and selenium binding are less at present, and the present invention provides the preparation method of a seed oyster selenium-enriched protein powder.
Invention content
One object of the present invention provides the preparation method of a seed oyster selenium-enriched protein powder, and the present invention is using oyster as original
Material prepares selenium rich of egg white powder, Zn content highest wherein in oyster, and Se content is relatively low, therefore the present invention adds by using oyster living
Add yeast selenium culture 10-15 days, increases the content of selenoprotein in oyster.
Technical solution provided by the invention is：
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, by oyster living in the yeast selenium sea containing 0.1-0.25% mass fractions
It is placed 10-15 days in water；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:10-50 is blended at a high speed, obtains oyster liquid, wherein the speed blended at a high speed is more than 5000 revs/min；
Step 3: adding protease weight ratio 100 into oyster liquid:0.5-1 is 30-50MPa, temperature 40- in pressure
Under conditions of 50 DEG C, stirs 1-2 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Preferably, it is also wrapped after oyster will be lived in step 1 in the yeast selenium seawater containing 0.1-0.25% mass fractions
It includes and is placed 1-2 hours in electromagnetic field daily, the intensity of electromagnetic field is the micro- special drawings of 1-2.
Preferably, it is also wrapped after oyster will be lived in step 1 in the yeast selenium seawater containing 0.1-0.25% mass fractions
The small-micelle water for adding seawater weight fraction 10-20% into seawater daily is included, after having added small-micelle water, agitation sea
Water 10-15 minutes.
Preferably, the oyster liquid that step 2 obtains is positioned in ultrasonic wave batch process 1-2 hours, ultrasonic frequency
It is 500-1000 hertz.
Preferably, the oyster liquid addition selenium-rich strain and yeast selenium obtained toward step 2, ferments 10-24 hours, wherein
The weight ratio of the oyster liquid, selenium-rich strain and yeast selenium is 100:0.1-0.5:0.1-0.5.
Preferably, the selenium-rich strain is made of following methods：By saccharomycete and lactic acid bacteria by weight 1:2 mixing,
0.5L sterile waters are added, then the PH that citric acid adjusts sterile water is used to add 30-50g sodium chloride and the Portugals 30-50g for 3.5-5
The mashed potato of grape sugar and 200g by gelatinization, is divided in the culture bottle of 100ml, temperature is controlled at 40-60 DEG C, is positioned over
After being cultivated 10-24 hours under oxygen-free environment, standard selenium solution 10-50ml is added in each culture bottle, the standard selenium solution
Culture bottle is placed in an oscillator after 1-3 minutes, then adjusts PH to 3.5-5 by a concentration of 200-500ug/ml, then is positioned over nothing
After cultivating 24-48 hours under oxygen environment, isolate and purify to obtain selenium-rich strain；Using the method turn out come selenium-rich strain.
Preferably, in step 4 after by Oyster Protein enzymolysis liquid enzyme deactivation further include the purification of Oyster Protein matter, purification
Specific steps include：After trichloroacetic acid-acetone of addition 10-50% weights stirs evenly, in placing response kettle, temperature is adjusted to 0
It is stir process 5-10 minutes under 1-5Mpa to 4 DEG C and pressure, temperature-resistant pressure is temperature after normal pressure is placed 10-20 minutes
Degree is adjusted to 0-4 DEG C and pressure is stir process 5-10 minutes under 1-5Mpa, and temperature-resistant pressure is that normal pressure places 10-20 points
Zhong Hou, circular treatment, stir process accumulated time is tied after being 40-60 minutes under being 1-5Mpa in temperature to 0 to 4 DEG C and pressure
Beam processing is placed into 500 turns/min in centrifuge and centrifuges 5-10 minutes, precipitated, pellet frozen is dried to obtain described male
Oyster selenium rich of egg white powder.
Beneficial effects of the present invention are as follows：
The present invention prepares selenium rich of egg white powder using oyster as raw material, Zn content highest wherein in oyster, and Se content compared with
It is low, therefore the present invention adds yeast selenium culture 10-15 days by using oyster living, increases the content of selenoprotein in oyster；It is preferred that
The condition in micro- electromagnetic field can more promote selenium exchange and oyster in albumen formed selenoprotein, significantly improve the selenium egg of oyster
Bai Hanliang, it is preferred to also have addition small-micelle water daily, using the unique package performance of small-micelle water by yeast selenium molecule
It wraps up and then forms small molecule, absorbed in a short time by oyster and be converted to selenoprotein, oyster can be remarkably promoted to yeast
The absorption of selenium, the present invention are also handled oyster liquid using selenium-rich strain, so as to significantly improve the oyster selenium rich of egg of preparation
Organic selenium content in white powder；The present invention also purifies albumen powder using trichloroacetic acid-acetone of 10-50% weights so that
The albumen powder purity of the present invention is high, and it is short efficient to purify the time.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail, to enable those skilled in the art's reference say
Bright book word can be implemented according to this.
Embodiment 1
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, oyster living is put in the yeast selenium seawater containing 0.1% mass fraction
It sets 10 days；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:10, it blends at a high speed, wherein 5500 revs/min of the speed blended at a high speed, obtains oyster liquid；
Step 3: adding protease weight ratio 100 into oyster liquid:0.5, it is 30MPa, the item that temperature is 40 DEG C in pressure
Under part, stirs 1 hour, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 2
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, by oyster living in the yeast selenium seawater containing 0.25% mass fraction
It places 15 days, is placed 2 hours in electromagnetic field daily, the intensity of electromagnetic field is 2 micro- special drawings；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:50, it blends at a high speed, obtains oyster liquid；
Step 3: adding protease weight ratio 100 into oyster liquid:1, it is 50MPa, the condition that temperature is 50 DEG C in pressure
Under, it stirs 2 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 3
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, oyster living is put in the yeast selenium seawater containing 0.2% mass fraction
It sets 12 days, adds the small-micelle water of seawater weight fraction 10% into seawater daily, after having added small-micelle water, agitation
Seawater 10 minutes；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:20, it blends at a high speed, obtains oyster liquid；
Step 3: adding protease weight ratio 100 into oyster liquid:0.7, it is 40MPa, the item that temperature is 45 DEG C in pressure
Under part, stirs 1.5 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 4
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, by oyster living in the yeast selenium seawater containing 0.15% mass fraction
It places 12 days, is placed 1.5 hours in electromagnetic field daily, the intensity of electromagnetic field is 1.5 micro- special drawings, adds sea into seawater daily
The small-micelle water of water weight fraction 15%, after having added small-micelle water, agitation seawater 12 minutes；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:25, it blends at a high speed, obtains oyster liquid；
Step 3: adding protease weight ratio 100 into oyster liquid:0.8, it is 45MPa, the item that temperature is 45 DEG C in pressure
Under part, stirs 1.5 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 5
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, oyster living is put in the yeast selenium seawater containing 0.2% mass fraction
It sets 12 days, is placed 1 hour in electromagnetic field daily, the intensity of electromagnetic field is 2 micro- special drawings, adds seawater weight into seawater daily
The small-micelle water of score 15%, after having added small-micelle water, agitation seawater 12 minutes；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:20, it blends at a high speed, obtains oyster liquid, oyster liquid is positioned over batch process 1 hour in ultrasonic wave, ultrasonic wave frequency
Rate is 800 hertz, and treated oyster liquid is added selenium-rich strain and yeast selenium, is fermented 20 hours, wherein the oyster liquid,
The weight ratio of selenium-rich strain and yeast selenium is 100:0.3:0.3；
Step 3: adding protease weight ratio 100 into oyster liquid:0.8, it is 45MPa, the item that temperature is 45 DEG C in pressure
Under part, stirs 1.5 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 6
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, oyster living is put in the yeast selenium seawater containing 0.1% mass fraction
It sets 10 days, is placed 1 hour in electromagnetic field daily, the intensity of electromagnetic field is 1 micro- special drawing, adds seawater weight into seawater daily
The small-micelle water of score 10-20%, after having added small-micelle water, agitation seawater 10 minutes；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:10, it blends at a high speed, obtains oyster liquid, oyster liquid is positioned over batch process 1 hour in ultrasonic wave, ultrasonic wave frequency
Rate is 500 hertz, and treated oyster liquid is added selenium-rich strain and yeast selenium, is fermented 10 hours, wherein the oyster liquid,
The weight ratio of selenium-rich strain and yeast selenium is 100:0.1:0.1, the selenium-rich strain is made of following methods：By saccharomycete and breast
Sour bacterium is by weight 1:2 mixing, are added 0.5L sterile waters, then use the PH of citric acid adjusting sterile water for 3.5, add 30g
The mashed potato of sodium chloride and 30g glucose and 200g by gelatinization, is divided in the culture bottle of 100ml, and temperature control exists
It 40 DEG C, is positioned over after being cultivated 10 hours under oxygen-free environment, standard selenium solution 10ml is added in each culture bottle, the standard selenium is molten
Culture bottle is placed in an oscillator after 1 minute, then adjusts PH to 3.5 by a concentration of 200ug/ml of liquid, then is positioned over anaerobic ring
After cultivating 24 hours under border, isolate and purify to obtain selenium-rich strain；Using the method turn out come selenium-rich strain；
Step 3: adding protease weight ratio 100 into oyster liquid:0.5, it is 30MPa, the item that temperature is 40 DEG C in pressure
Under part, stirs 1 hour, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
Embodiment 7
The preparation method of one seed oyster selenium-enriched protein powder, the preparation method include：
Step 1: selecting oyster living as raw material, by oyster living in the yeast selenium seawater containing 0.25% mass fraction
It places 15 days, is placed 2 hours in electromagnetic field daily, the intensity of electromagnetic field is 2 micro- special drawings, adds seawater weight into seawater daily
The small-micelle water for measuring score 20%, after having added small-micelle water, agitation seawater 15 minutes；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water
By weight 10:50, it blends at a high speed, obtains oyster liquid, oyster liquid is positioned over batch process 2 hours in ultrasonic wave, ultrasonic wave frequency
Rate is 1000 hertz, and treated oyster liquid is added selenium-rich strain and yeast selenium, is fermented 24 hours, wherein the oyster
The weight ratio of liquid, selenium-rich strain and yeast selenium is 100:0.5:0.5, the selenium-rich strain is made of following methods：By saccharomycete
With lactic acid bacteria by weight 1:2 mixing, are added 0.5L sterile waters, then use the PH of citric acid adjusting sterile water for 5, add
The mashed potato of 50g sodium chloride and 50g glucose and 200g by gelatinization, is divided in the culture bottle of 100ml, temperature control
It at 60 DEG C, is positioned over after being cultivated 24 hours under oxygen-free environment, standard selenium solution 50ml, the standard selenium is added in each culture bottle
Culture bottle is placed in an oscillator after 3 minutes, then adjusts PH to 5 by a concentration of 500ug/ml of solution, then is positioned over anaerobic ring
After cultivating 48 hours under border, isolate and purify to obtain selenium-rich strain；Using the method turn out come selenium-rich strain；
Step 3: adding protease weight ratio 100 into oyster liquid:1, it is 50MPa, the condition that temperature is 50 DEG C in pressure
Under, it stirs 2 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
To further include Oyster Protein matter after Oyster Protein enzymolysis liquid enzyme deactivation Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation
Purification, the specific steps of purification include：After trichloroacetic acid-acetone of 50% weight of addition stirs evenly, in placing response kettle, temperature
It is stir process 10 minutes under 5Mpa to be adjusted to 4 DEG C and pressure, and temperature-resistant pressure is that normal pressure is placed after twenty minutes, temperature tune
Section to 4 DEG C and pressure are stir process 10 minutes under 5Mpa, and temperature-resistant pressure is that normal pressure is placed after twenty minutes, at cycle
Reason, stir process accumulated time is to end processing after sixty minutes under being 5Mpa in temperature to 4 DEG C and pressure, is placed into centrifuge
In 500 turns/min centrifuge 10 minutes, precipitated, pellet frozen be dried to obtain the oyster selenium rich of egg white powder.
Comparative example 1
It is same as Example 1, lack step 1.
Oyster selenium-enriched protein powder content is tested using Coomassie Brilliant Blue (Bradford methods), calculates oyster selenium rich of egg white powder
Recovery rate and oyster selenium-enriched protein powder purity utilize the male of efficient liquid phase-Hydride Generation-atomic Fluorescence Spectrometry analysis extraction
Total Se content in oyster selenium rich of egg white powder and organic selenium content, as a result such as following table：
| Selenium rich of egg white powder recovery rate | Selenium-enriched protein powder purity | Organic selenium content | Total Se content | |
| Embodiment 1 | 53.5% | 90.4% | 7.54mg/kg | 8.21mg/kg |
| Embodiment 2 | 53.4% | 90.2% | 11.35mg/kg | 12.20mg/kg |
| Embodiment 3 | 52.8% | 91.4% | 12.45mg/kg | 13.53mg/kg |
| Embodiment 4 | 53.9% | 90.9% | 14.42mg/kg | 15.51mg/kg |
| Embodiment 5 | 54.1% | 90.7% | 16.01mg/kg | 17.25mg/kg |
| Embodiment 6 | 53.2% | 91.2% | 16.58mg/kg | 17.64mg/kg |
| Embodiment 7 | 52.1% | 94.5% | 16.39mg/kg | 17.82mg/kg |
| Comparative example 1 | 52.1% | 90.1% | 0.85mg/kg | 0.92mg/kg |
By data in table as it can be seen that the method for the present invention can significantly improve the content of Organic Selenium in oyster selenium rich of egg white powder, have
The content ratio of machine selenium is up to 90% or more.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (7)
1. the preparation method of a seed oyster selenium-enriched protein powder, which is characterized in that the preparation method includes：
Step 1: selecting oyster living as raw material, by oyster living in the yeast selenium seawater containing 0.1-0.25% mass fractions
It places 10-15 days；
Step 2: oyster living is won oyster meat, the internal organ of oyster meat are cleaned up, then by oyster meat and deionized water by weight
Measure ratio 10:10-50 is blended at a high speed, obtains oyster liquid；
Step 3: adding protease weight ratio 100 into oyster liquid:0.5-1 is 30-50MPa in pressure, and temperature is 40-50 DEG C
Under conditions of, it stirs 1-2 hours, Oyster Protein enzymolysis liquid is obtained by filtration；
Step 4: by after Oyster Protein enzymolysis liquid enzyme deactivation, the oyster selenium rich of egg white powder is obtained by freeze-drying.
2. the preparation method of oyster selenium rich of egg white powder as described in claim 1, which is characterized in that oyster living exists in step 1
Further include being placed 1-2 hours in electromagnetic field daily after in yeast selenium seawater containing 0.1-0.25% mass fractions, electromagnetic field
Intensity to be that 1-2 is micro- special draw.
3. the preparation method of oyster selenium rich of egg white powder as described in claim 1, which is characterized in that oyster living exists in step 1
Further include adding seawater weight fraction 10- into seawater daily after in yeast selenium seawater containing 0.1-0.25% mass fractions
20% small-micelle water, after having added small-micelle water, agitation seawater 10-15 minutes.
4. the preparation method of oyster selenium rich of egg white powder as described in claim 1, which is characterized in that the oyster for obtaining step 2
Liquid is positioned in ultrasonic wave batch process 1-2 hours, and ultrasonic frequency is 500-1000 hertz.
5. the preparation method of oyster selenium rich of egg white powder as described in claim 1, which is characterized in that the oyster obtained toward step 2
Liquid adds selenium-rich strain and yeast selenium, ferments 10-24 hours, wherein the weight ratio of the oyster liquid, selenium-rich strain and yeast selenium
It is 100:0.1-0.5:0.1-0.5.
6. the preparation method of oyster selenium rich of egg white powder as claimed in claim 5, which is characterized in that the selenium-rich strain is by following
Method is made：By saccharomycete and lactic acid bacteria by weight 1:0.5L sterile waters are added in 2 mixing, then sterile using citric acid adjusting
The PH of water is 3.5-5, adds the mashed potato of 30-50g sodium chloride and 30-50g glucose and 200g by gelatinization, packing
In the culture bottle of 100ml, temperature is controlled at 40-60 DEG C, is positioned over after being cultivated 10-24 hours under oxygen-free environment, each to cultivate
Culture bottle is placed on and shakes by addition standard selenium solution 10-50ml, a concentration of 200-500ug/ml of the standard selenium solution in bottle
It swings in device after 1-3 minutes, then adjusts PH to 3.5-5, then after being positioned over and being cultivated 24-48 hours under oxygen-free environment, isolate and purify
To selenium-rich strain.
7. the preparation method of oyster selenium rich of egg white powder as described in claim 1, which is characterized in that by oyster egg in step 4
Further include the purification of Oyster Protein matter after white enzymolysis liquid enzyme deactivation, the specific steps of purification include：The trichlorine vinegar of 10-50% weights is added
After acid-acetone stirs evenly, in placing response kettle, temperature is adjusted to 0 to 4 DEG C and pressure is stir process 5-10 under 1-5Mpa
Minute, temperature-resistant pressure is after normal pressure is placed 10-20 minutes, and it is to be stirred under 1-5Mpa that temperature, which is adjusted to 0-4 DEG C and pressure,
Processing 5-10 minutes, temperature-resistant pressure are after normal pressure is placed 10-20 minutes, and circular treatment to 0 to 4 DEG C and is pressed in temperature
Power is that stir process accumulated time ends processing after being 40-60 minutes under 1-5Mpa, is placed into 500 turns/min in centrifuge and centrifuges
It 5-10 minutes, is precipitated, pellet frozen is dried to obtain the oyster selenium rich of egg white powder.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269130A (en) * | 2019-05-22 | 2019-09-24 | 浙江国际海运职业技术学院 | The preparation method of selenium-rich oyster small molecular protein peptide |
| CN112778397A (en) * | 2021-01-28 | 2021-05-11 | 北部湾大学 | Method for extracting selenium-containing protein from oysters by using off-site enhanced mechanical ball milling method to assist salt dissolution |
| CN114365711A (en) * | 2022-01-24 | 2022-04-19 | 威海金牌生物科技有限公司 | Selenium-rich oyster cultivation method |
-
2018
- 2018-04-11 CN CN201810321799.2A patent/CN108477377A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269130A (en) * | 2019-05-22 | 2019-09-24 | 浙江国际海运职业技术学院 | The preparation method of selenium-rich oyster small molecular protein peptide |
| CN112778397A (en) * | 2021-01-28 | 2021-05-11 | 北部湾大学 | Method for extracting selenium-containing protein from oysters by using off-site enhanced mechanical ball milling method to assist salt dissolution |
| CN114365711A (en) * | 2022-01-24 | 2022-04-19 | 威海金牌生物科技有限公司 | Selenium-rich oyster cultivation method |
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