CN108450952A - A kind of cell-repairing oral liquid - Google Patents

A kind of cell-repairing oral liquid Download PDF

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Publication number
CN108450952A
CN108450952A CN201810127149.4A CN201810127149A CN108450952A CN 108450952 A CN108450952 A CN 108450952A CN 201810127149 A CN201810127149 A CN 201810127149A CN 108450952 A CN108450952 A CN 108450952A
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cell
bird
nest
extract
oral liquid
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陈维雯
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GUANGZHOU WEIZI TRADE Co.,Ltd.
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Shenzhen Only Posture Trade Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/756—Phellodendron, e.g. corktree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8964—Anemarrhena
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention discloses a kind of cell-repairing oral liquids, including following raw material：Bird's nest extracts 10 ~ 30 parts of collagen, 1 ~ 5 part of natural ferment, 50 ~ 80 parts of purified water, 1 ~ 10 part of corrigent.Reinforce digesting and absorbing for food, improve immunity, achievees the effect that improve cell repair ability.

Description

A kind of cell-repairing oral liquid
Technical field
The present invention relates to field of food, and in particular to a kind of cell-repairing oral liquid and preparation method thereof.
Background technology
Passion fruit extract has antianxiety and treatment insomnia, can slow down food by gastral speed, to promote The effect of digestion.
Natural ferment can promote the digestion of food, adjust the possibility liquid matter of microorganism, and natural equilibrium is original abundant micro- Ingredient is measured, the metabolism of cell, enhancing muscle power can be promoted to promote the cell neogenesis to wreck, contribute to the reparation of cell.
Bird's nest is several birds of Apodidae Apodidae aerodramuss Collocalia, and eat marine small fish or other silkworms After the digestion of the atoms such as spiral shell seaweed, nest that the gastric juice that secrets out of is mixed and condensed on overhanging cliff with its down, and builds up.Swallow Nest is the rare tonic one of eponymous with ginseng, pilose antler.
Rhizoma anemarrhenae, Cortex Phellodendri have the effect of heat-clearing, purging intense heat, removing toxic substances.
It is living containing a variety of biologies such as a large amount of mucoproteins, glycoprotein, sialic acid, trace element, epidermal growth factor in bird's nest Property ingredient, have it is antiviral, promote cell division, improve immunity.
On the one hand traditional eating method is to stew or be processed into bird's nest tin, and bird's nest keeps original high molecular weight protein knot substantially Structure, molecular weight is more than hundreds of thousands dalton, the characteristics of there are water-insolubles so that absorption of human body utilization rate is low, the prior art The peptide fragment of enzymolysis bird's nest is concentrated mainly on 1000 or more；On the other hand, especially small molecule has the characteristics that:
(1) it is not required to digest, directly absorb.
(2) consumption body energy is not required to when absorbing.
(3) human intestines and stomach's function burden is not increased.
(4) has the characteristics that preferential absorption.
(5) it absorbs quickly, 129600 seconds faster than absorption of human body macro-molecular protein, 64800 seconds fast compared with human amino acid.
(6) it is pushed and is absorbed with self-energy.
(7) 100% are absorbed.
(8) when absorption of human body function reduces, human body being capable of Passive intake.
(9) there is carrier function, the other nutrition of human body can be loaded on matrix, be transported to human cell tissue.
(10) there is extremely strong activity and physiological function diversity.
Existing cell-repairing oral liquid absorbs, effect is not ideal enough, provides a kind of cell for being easy to absorb, effect is good and repaiies Multiple oral solution is urgently to be resolved hurrily.
Invention content
To solve existing cell-repairing oral liquid absorption and the not ideal enough technical problem of effect, a kind of cell repair is provided Oral solution, including bird's nest extracts collagen, molecular weight is concentrated mainly on 180 ~ 500, is easy to absorb, with passion fruit extract, Natural ferment, rhizoma anemarrhenae, Cortex Phellodendri are used in combination, and reinforce digesting and absorbing for food, improve immunity, reach and improve cell and repair Multiple effect.
To realize above-mentioned technical problem, it is achieved by following technical solution：
A kind of cell-repairing oral liquid, including following raw material：Bird's nest extracts 10 ~ 30 parts of collagen, natural ferment 1 ~ 5 Part, 50 ~ 80 parts of purified water, 1 ~ 10 part of corrigent.
Preferably, the cell-repairing oral liquid also includes the raw material of following parts by weight：2 ~ 10 parts of anemarrhena asphodefoides extract, Cortex Phellodendri 2 ~ 5 parts of extract, 20 ~ 50 parts of passion fruit extract.
Preferably, the preparation method of the anemarrhena asphodefoides extract is：8 ~ 12KG water is added in 1KG rhizoma ane marrhenaes, and 50 ~ 100 DEG C carry 4 ~ 8h, extracting solution filtering is taken to obtain 200 ~ 300g medicinal extract after filtrate concentration；
The preparation method of the phellodendron extract is：The Cortex Phellodendri medicinal material of 1KG, is added 8 ~ 12KG water, and 60 ~ 100 DEG C of 4 ~ 8h of extraction are carried It takes liquid to filter, 200 ~ 300g medicinal extract is obtained after filtrate concentration；
The preparation method of the passion fruit extract is：The pulp of 1KG passion fruits is smashed, is filtered, 100 are obtained after filtrate concentration ~ 200g medicinal extract.
Preferably, the Gaussian Profile peak area percent that the bird's nest extraction collagen molecules amount is 500 ~ 180 is swallow It is 5000 ~ 1000 that 3 times of the Gaussian Profile peak area percent of nest extraction collagen molecules amount 1000-500, which are molecular weight, 7 times of the Gaussian Profile peak area percent of polypeptide.
Preferably, the molecular weight of bird's nest extraction collagen has following Gaussian Profile：
Molecular weight ranges Peak area percent（%, λ=220nm）
>10000 3
10000~5000 5
5000~1000 8
1000~500 19
500~180 57
<180 8
Preferably, the preparation method of the bird's nest extraction collagen is：
Step 1: cleaning：Fresh bird's nest is cleaned up, 1 is pressed with deionized water:20~1:50 mixing, are dipped to bird's nest and have absorbed water Entirely；
Step 2: denaturation：Soak is warming up to 90 ~ 95 DEG C, is kept for 10 ~ 15 minutes, makes protein denaturation；
Step 3: enzymolysis：Soak is cooled to 30 ~ 45 DEG C, recombinant protein is added by 0.05% ~ 0.5% weight of bird's nest dry weight Enzyme adjusts pH to 5 ~ 6.5,4 ~ 6 hours is kept the temperature at 37 ~ 45 DEG C, and PH is nature PH, is not required to control
Step 4: inactivation：80 ~ 90 DEG C keep the temperature 2 ~ 4 hours；
Step 5: filtering to obtain the yellowish liquid of clarification；
Step 6: concentration, is spray-dried or is freeze-dried to obtain pale yellow powder.
Preferably, the DNA sequences of the complete genome of the recombinant protease are：
ACGAATGATGTTGGGGGGGGCGGATACATCGATAAAGCTCCTAGTCAAAGAAAAATTGTTAGCGTTACA
TTAACCGCTCAAGTACACAGGCATATTGCTCATTGTCTTGTTGATAATATATCAGCAAGAGTTGGTAGT
GATTATGATATAGCAATCCGAACTTACGTGGCTATTTTGACATTCTCACAAGATGACGGTGTCAACACT
GGCGGTGATGCAGTTAATTGGGGAGCTACATCGGAATCCTCAAAAACATTGTTGGTTCCTACTCTATCC
GCTGTTAGCGGTGGTCCGGGAAAAGATGCTTGCCAGGGAGACTCTAATAATGTACAATTGGGAGTTGTC
ATTTTTTGGGCTTGTGCCATGTACAAGTATTATTTTTTACTATTGAAAGATGGTAGCTGTAAGAAAAGT
TACTTGCTTTGTGGGGGATACCAAGCGTTAGAAAAGGGTAAGGAGTATTACCAATCCATCATCAGCAAA
GATAAAGGCACTATACACGGTAAAGTGACGAATAACTATTCGACCAAATCGATGCCCAATTATAATTCT
ACACTACCGGTACCTGCGAAAATCGTAAGTGAAAACAATGGGGCTAAGACAACATTATTTGTCACCGGT
GACAGAATGAGTGTGTCATGCAAAAAAGTAGCAGAAAACTTTTCTCTTTGTGCAGGCGTCTCTCAAGGA
TCATTTGGAAGTGGCTGTGCCCGTCCTGGAAACCCTGGTGTATATGCAAATGTCACAGCACTCCGTGGT
TGGATACGAAAGACTGCAGGAGTTTTA
Preferably, the corrigent is table sugar or xylitol.
Preferably, a kind of preparation method of cell-repairing oral liquid, it is characterised in that：
（1）Bird's nest is extracted into collagen, hundred natural ferment, corrigent, anemarrhena asphodefoides extract, phellodendron extract, hundred by weight Fragrant berry extract, water are uniformly mixed；
（2）By step（1）Gained mixture liquid filters, and encapsulation, heat treated sterilization to obtain the final product.
8. preparation method according to claim 7, which is characterized in that the filtering uses sieve for 5 ~ 10 mesh.
Advantageous effect：
Used in the present invention bird's nest extraction collagen main molecules amount concentrate on 180 ~ 500, due to its for dipeptides to tripeptides, can It directly to be absorbed by gastrointestinal tract, need not decompose, absorptivity is up to 99%, has very high utilization rate.Bird's nest extracts collagen egg It is used in combination in vain with passion fruit extract, natural ferment, rhizoma anemarrhenae, Cortex Phellodendri, reinforces the digesting and absorbing of food, improved immune Power contributes to the raising of cell repair ability.
Specific embodiment
Embodiment 1
The preparation of recombinant protease
One, the structure of engineering bacteria
The DNA sequences of the complete genome of recombinant protease are：
ACGAATGATGTTGGGGGGGGCGGATACATCGATAAAGCTCCTAGTCAAAGAAAAATTGTTAGCGTTACA
TTAACCGCTCAAGTACACAGGCATATTGCTCATTGTCTTGTTGATAATATATCAGCAAGAGTTGGTAGT
GATTATGATATAGCAATCCGAACTTACGTGGCTATTTTGACATTCTCACAAGATGACGGTGTCAACACT
GGCGGTGATGCAGTTAATTGGGGAGCTACATCGGAATCCTCAAAAACATTGTTGGTTCCTACTCTATCC
GCTGTTAGCGGTGGTCCGGGAAAAGATGCTTGCCAGGGAGACTCTAATAATGTACAATTGGGAGTTGTC
ATTTTTTGGGCTTGTGCCATGTACAAGTATTATTTTTTACTATTGAAAGATGGTAGCTGTAAGAAAAGT
TACTTGCTTTGTGGGGGATACCAAGCGTTAGAAAAGGGTAAGGAGTATTACCAATCCATCATCAGCAAA
GATAAAGGCACTATACACGGTAAAGTGACGAATAACTATTCGACCAAATCGATGCCCAATTATAATTCT
ACACTACCGGTACCTGCGAAAATCGTAAGTGAAAACAATGGGGCTAAGACAACATTATTTGTCACCGGT
GACAGAATGAGTGTGTCATGCAAAAAAGTAGCAGAAAACTTTTCTCTTTGTGCAGGCGTCTCTCAAGGA
TCATTTGGAAGTGGCTGTGCCCGTCCTGGAAACCCTGGTGTATATGCAAATGTCACAGCACTCCGTGGT
TGGATACGAAAGACTGCAGGAGTTTTA。
With in conventional molecular biology method structure enzyme gene containing recombinant protein to e. coli bl21 (DE3), build Recombinant protein enzyme engineering bacteria.
Two, it ferments：Recombination engineering ferments, in the LB culture mediums of 200 μ l to 20ml of inoculation recombination engineering positive colony, Shaking table shaken cultivation is stayed overnight at 37 DEG C, takes over night bacterium in the LB culture mediums of 500ml by 5 ‰ inoculum concentrations, shaking table shakes at 37 DEG C It swings culture and arrives logarithmic phase, be inoculated into the fermentation tank equipped with 6L culture mediums, initial parameter is：37 DEG C of fermentation temperature, mixing speed Speed of agitator and ventilatory capacity (rotating speed maximum 1000rpm, ventilatory capacity is continuously improved in 350rpm, ventilatory capacity 20L/min, pH 6.8 Maximum 35L/min) to maintain dissolved oxygen always 30% or more, culture medium prescription is as follows：
Fermentation medium：Glucose 6g/L, yeast extract powder 25g/L, disodium hydrogen phosphate dodecahydrate 10g/L, potassium dihydrogen phosphate 4g/L, ammonium sulfate 6g/L, epsom salt 1.13g/L, calcium chloride dihydrate 0.073g/L, supplemented medium：Yeast extract powder 25g/L, glucose 35g/L, 50% ammonium hydroxide and 30% phosphoric acid are used for pH in fermentation process and regulate and control.
When nutrient depletion in culture medium, start feed supplement when dissolved oxygen rises rapidly, the rotating speed by controlling pump rationally controls benefit Expect speed, while feed rate, rotating speed, ventilation three it is reasonable control under, ensure control dissolved oxygen 30% or more.Feed supplement 6h Afterwards, 23 DEG C are cooled to, pH to 7.5 is adjusted, the aerobic induction of IPTG progress that final concentration of 0.1mmol/L is added (ensures molten Oxygen goes out tank after being not less than 20%) 8h.Go out after tank and centrifuges to obtain thalline weight in wet base 160g/L, thalli growth curve and correlation in fermentation process Sample point electrophoresis, expression quantity reach 60% or more of whole bacterial protein.The thalline that fermentation ends are collected by centrifugation volume ratio 1 by weight：10 add Enter disruption buffer (25mmol/L Tris-HCl+5mmol/LEDTA), collected after high pressure homogenizer 800bar is broken precipitation and Supernatant electrophoresis, inclusion body purity recombinant protein proenzyme are more than 85%.
Three, the purifying of recombinant protein proenzyme
After fermentation, thalline were collected by centrifugation, by weight volume ratio 1：10 are added disruption buffer (25mmol/L Tis-HCl+ 5mmol/L EDTA, PH7.5), it is crushed twice under the conditions of high pressure homogenizer 800bar, inclusion body precipitation, inclusion body is collected by centrifugation Weight in wet base is 30g/L zymotic fluids.It will precipitation volume ratio 1 by weight：15 are added washing buffer (2mol/L urea+2.0% Triton), room temperature magnetic agitation 1 hour, the precipitation being collected by centrifugation are washed twice with washing buffer.It is slow with solubilization of inclusion bodies again Fliud flushing (8mol/L urea+10mmol/L EDTA+25mmol/L Tris-HCl, PH7.5) volume ratio 1 by weight：10 dissolvings Overnight.Dissolved inclusion body is diluted to albumen concentration 0.2mg/ml, in renaturation buffer after centrifugation, ultrafiltration removal impurity (0.2mol/L urea+10mmol/L CaCl2+ 50mmol/L Tris-HCl, GSH：GSSG=1mmol/L：0.5mmol/ L, PH10) in, 4 DEG C of renaturation are stayed overnight, and the recombinant protein original after renaturation is through protein kinase (1：150) after digesting 6 hours at room temperature It can be obtained active crude enzyme liquid.Crude enzyme liquid is through hydrophobic chromatography, condition：Hydrophobic chromatography condition：Buffer solution A -50mM Tris-HCl+1.5M(NH4)2SO4, pH8.0；Buffer solution B -50mM Tris-HCl, pH8.0；0-100% B linear gradients 50CV ；Ion exchange, condition are：Buffer solution A -50mM Tris-HCl, pH8.0；Buffer solution B -20mM Tris-HCl+2M NaCl, pH8.0；0-100%B linear gradients 20CV；It can be obtained with high-purity and activated protein enzyme product.
The recombinant protease of purifying reaches 95% or more through electrophoretic analysis, purity.
A kind of preparation of 2 cell-repairing oral liquid of embodiment
A kind of cell-repairing oral liquid, including following raw material：Bird's nest extracts 10 parts of collagen, passion fruit extract 20 Part, 1 part of natural ferment, 50 parts of purified water, 2 parts of anemarrhena asphodefoides extract, 2 parts of phellodendron extract, 1 part of corrigent.
The preparation method of the anemarrhena asphodefoides extract is：8KG water, 50 DEG C of extraction 4h, extracting solution mistake is added in 1KG rhizoma ane marrhenaes Filter obtains 200g medicinal extract after filtrate concentration；
The preparation method of the phellodendron extract is：8KG water, 60 DEG C of extraction 4h is added in the Cortex Phellodendri medicinal material of 1KG, and extracting solution filters, 200g medicinal extract is obtained after filtrate concentration；
The preparation method of the passion fruit extract is：The pulp of 1KG passion fruits is smashed, is filtered, is obtained after filtrate concentration 100g medicinal extract.
The Gaussian Profile peak area percent that the bird's nest extraction collagen molecules amount is 500 ~ 180 is bird's nest extraction glue 2 ~ 3 times of the Gaussian Profile peak area percent of former molecular weight of albumen 1000-500 are the polypeptides that molecular weight is 5000 ~ 1000 5 ~ 7 times of Gaussian Profile peak area percent.
The preparation method of bird's nest extraction collagen is：
Step 1: cleaning：Fresh bird's nest is cleaned up, 1 is pressed with deionized water:It is complete to be dipped to bird's nest water suction for 20 mixing；
Step 2: denaturation：Soak is warming up to 90 DEG C, is kept for 10 minutes, makes protein denaturation；
Step 3: enzymolysis：Soak is cooled to 30 DEG C, recombinant protease is added by 0.05% weight of bird's nest dry weight, adjusts pH To 5,4 hours are kept the temperature at 37 DEG C, PH is nature PH, is not required to control；
Step 4: inactivation：80 DEG C keep the temperature 2 hours；
Step 5: filtering to obtain the yellowish liquid of clarification;
Step 6: concentration, is spray-dried or is freeze-dried to obtain pale yellow powder.
The recombinant protease is identical with recombinant protease in embodiment 1.
The preparation method of cell-repairing oral liquid as described above, comprises the following steps：
（1）Bird's nest is extracted into collagen, hundred natural ferment, corrigent, anemarrhena asphodefoides extract, phellodendron extract, hundred by weight Fragrant berry extract, water are uniformly mixed；
（2）By step（1）Gained mixture liquid filters, and encapsulation, heat treated sterilization to obtain the final product.
A kind of preparation of 3 cell-repairing oral liquid of embodiment
A kind of cell-repairing oral liquid, including following raw material：Bird's nest extracts 20 parts of collagen, passion fruit extracting solution 30 Part, 3 parts of natural ferment, 60 parts of purified water, 8 parts of rhizoma anemarrhenae, 3 parts of Cortex Phellodendri, 3 parts of xylitol.
The preparation method of the anemarrhena asphodefoides extract is：10KG water, 80 DEG C of extraction 6h, extracting solution is added in 1.5KG rhizoma ane marrhenaes Filtering obtains 250g medicinal extract after filtrate concentration；
The preparation method of the phellodendron extract is：10KG water, 90 DEG C of extraction 6h, extracting solution mistake is added in the Cortex Phellodendri medicinal material of 1.5KG Filter obtains 250g medicinal extract after filtrate concentration；
The preparation method of the passion fruit extract is：The pulp of 1.5KG passion fruits is smashed, is filtered, is obtained after filtrate concentration 150g medicinal extract.
The bird's nest extraction collagen molecules amount Gaussian Profile peak area percent is as follows：
Molecular weight ranges Peak area percent（%, λ=220nm）
>10000 3
10000~5000 5
5000~1000 8
1000~500 19
500~180 57
<180 8
The preparation method of bird's nest extraction collagen is：
Step 1: cleaning：Fresh bird's nest is cleaned up, 1 is pressed with deionized water:It is complete to be dipped to bird's nest water suction for 40 mixing；
Step 2: denaturation：Soak is warming up to 90 DEG C, is kept for 10 minutes, makes protein denaturation；
Step 3: enzymolysis：Soak is cooled to 40 DEG C, recombinant protease is added by 0.2% weight of bird's nest dry weight, adjusts pH To 6,5 hours are kept the temperature at 40 DEG C, PH is nature PH, is not required to control
Step 4: inactivation：85 DEG C keep the temperature 3 hours；
Step 5: filtering to obtain the yellowish liquid of clarification;
Step 6: concentration, is spray-dried or is freeze-dried to obtain pale yellow powder.
The recombinant protease is same as Example 1.
The preparation method of cell-repairing oral liquid as described above, including following preparation process：
（1）Bird's nest is extracted into collagen, hundred natural ferment, corrigent, anemarrhena asphodefoides extract, phellodendron extract, hundred by weight Fragrant berry extract, water are uniformly mixed；
（2）By step（1）Gained mixture liquid filters, and encapsulation, heat treated sterilization to obtain the final product.
The filtering uses sieve for 10 mesh.
A kind of preparation of 4 cell-repairing oral liquid of embodiment
A kind of cell-repairing oral liquid, including following raw material：Bird's nest extracts 30 parts of collagen, passion fruit extracting solution 50 Part, 5 parts of natural ferment, 80 parts of purified water, 10 parts of rhizoma anemarrhenae, 5 parts of Cortex Phellodendri, 10 parts of table sugar.
The preparation method of the anemarrhena asphodefoides extract is：12KG water, 100 DEG C of extraction 8h, extraction is added in 2KG rhizoma ane marrhenaes Liquid filters, and 300g medicinal extract is obtained after filtrate concentration；
The preparation method of the phellodendron extract is：12KG water, 100 DEG C of extraction 8h, extracting solution mistake is added in the Cortex Phellodendri medicinal material of 2KG Filter obtains 300g medicinal extract after filtrate concentration；
The preparation method of the passion fruit extract is：The pulp of 2KG passion fruits is smashed, is filtered, is obtained after filtrate concentration 200g medicinal extract.
The Gaussian Profile peak area percent that the bird's nest extraction collagen molecules amount is 500 ~ 180 is bird's nest extraction glue 3 times of the Gaussian Profile peak area percent of former molecular weight of albumen 1000-500 are the height for the polypeptide that molecular weight is 5000 ~ 1000 8 times of this distribution peak area percent.
Preferably, the preparation method of the bird's nest extraction collagen is：
Step 1: cleaning：Fresh bird's nest is cleaned up, 1 is pressed with deionized water:It is complete to be dipped to bird's nest water suction for 50 mixing；
Step 2: denaturation：Soak is warming up to 95 DEG C, is kept for 15 minutes, makes protein denaturation；
Step 3: enzymolysis：Soak is cooled to 45 DEG C, recombinant protease is added by 0.5% weight of bird's nest dry weight, adjusts pH To 6.5,6 hours are kept the temperature at 45 DEG C, PH is nature PH, is not required to control
Step 4: 90 DEG C of inactivation keeps the temperature 2 ~ 4 hours；
Step 5: filtering to obtain the yellowish liquid of clarification；
Step 6: concentration, is spray-dried or is freeze-dried to obtain pale yellow powder.
The recombinant protease is same as Example 1
A kind of preparation method of cell-repairing oral liquid, including following preparation process：
（1）Bird's nest is extracted into collagen, hundred natural ferment, corrigent, anemarrhena asphodefoides extract, phellodendron extract, hundred by weight Fragrant berry extract, water are uniformly mixed；
（2）By step（1）Gained mixture liquid filters, and encapsulation, heat treated sterilization to obtain the final product.
The cell-repairing oral liquid application method and effect of the present invention is as follows:
1. normal person beauty and health care person:It takes 2 (50 milliliters every) daily, respectively takes 1 sooner or later.
A large amount of replenishing collagens make application on human skin high resilience, preferably using 6-12 months.
2. there is the patient of cardiopulmonary and vascular disease, 4 are taken daily, respectively takes 2, after taking 3 months sooner or later, symptom is apparent Mitigate, after taking half a year, cardio-pulmonary function is obviously improved.
3. inpatient with haematological diseases (various inpatient with haematological diseases):3 are taken daily, 1 is respectively taken in the morning, afternoon and evening, it is bright to take rear symptom Aobvious to mitigate, clinical symptoms and every laboratory indexes are gradually normal in 1 year, are detached from clothes
Use western medicine.
4. special serious pulmonary tuberculosis, emphysema patient:6 are taken daily, respectively takes 2 in the morning, afternoon and evening,
To consumptive, cured substantially in 1-2.
5. skin disease (psoriasis patient) and allergic people:3 are taken daily, 2 early, evening 1
Branch.1-2 is cured substantially, and observation in the recent period is not recurred.
6. skin nursing and young man:1 is taken daily, skin can be improved by taking throughout the year, protect the youth,
Keep the youth resident.The young man month is civilian within 30 years old, can be obviously improved within 2 months skin in a short time, makes complete in vivo Face is vigorous, achievees the purpose that control in outer disease.
Clinical report:
1. beauty:It is observed after selecting 50 people of female, male 50 people to take 6 months, the skin person of being obviously improved 90
People, 100 people of responder, effective percentage 100%.
2. cardiopulmonary and vascular disease:100 people are selected to take, after taking half a year, hence it is evident that 80 people of improvement, effective 98 people, invalid 2 people, Effective percentage 98%.
3. blood disease:After selecting 100 people to take half a year, normal 80 people of indices recovery, effective 95
People, invalid 5 people, effective percentage 95%.
4. pulmonary tuberculosis, emphysema patient:After 100 people take half a year, hence it is evident that 90 people of improvement, effective 97
People, invalid 3 people, effective percentage 97%.
5. skin disease:After 100 people take 1 year, hence it is evident that 93 people of improvement, effective 100 people, invalid 0 people,
Effective percentage 100%.
Although being related to a kind of cell-repairing oral liquid to disclosed with reference to embodiment and being particularly described,
Embodiments described above is illustrative rather than restrictive, the case where not departing from the spirit and scope of the present invention Under, all change and modification are within the scope of the present invention.
SEQUENCE LISTING
<110>Wei Zi trade Co., Ltds of Shenzhen
<120>Recombinant protease
<130>
<160> 1
<170>PatentIn version 3.5
<210> 1
<211> 786
<212> DNA
<213>Artificial sequence (Artificial sequence)
<400> 1
acgaatgatgttggggggggcggatacatcgataaagctcctagtcaaagaaaaattgtt 60
agcgttacattaaccgctcaagtacacaggcatattgctcattgtcttgttgataatata 120
tcagcaagagttggtagtgattatgatatagcaatccgaacttacgtggctattttgaca 180
ttctcacaagatgacggtgtcaacactggcggtgatgcagttaattggggagctacatcg 240
gaatcctcaaaaacattgttggttcctactctatccgctgttagcggtggtccgggaaaa 300
gatgcttgccagggagactctaataatgtacaattgggagttgtcattttttgggcttgt 360
gccatgtacaagtattattttttactattgaaagatggtagctgtaagaaaagttacttg 420
ctttgtgggggataccaagcgttagaaaagggtaaggagtattaccaatccatcatcagc 480
aaagataaaggcactatacacggtaaagtgacgaataactattcgaccaaatcgatgccc 540
aattataattctacactaccggtacctgcgaaaatcgtaagtgaaaacaatggggctaag 600
acaacattatttgtcaccggtgacagaatgagtgtgtcatgcaaaaaagtagcagaaaac 660
ttttctctttgtgcaggcgtctctcaaggatcatttggaagtggctgtgcccgtcctgga 720
aaccctggtgtatatgcaaatgtcacagcactccgtggttggatacgaaagactgcagga 780
gtttta 786
Sequence table
<110>Wei Zi trade Co., Ltds of Shenzhen
<120>A kind of cell-repairing oral liquid
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 786
<212> DNA
<213>Artificial sequence (Artificial sequence)
<400> 1
acgaatgatg ttgggggggg cggatacatc gataaagctc ctagtcaaag aaaaattgtt 60
agcgttacat taaccgctca agtacacagg catattgctc attgtcttgt tgataatata 120
tcagcaagag ttggtagtga ttatgatata gcaatccgaa cttacgtggc tattttgaca 180
ttctcacaag atgacggtgt caacactggc ggtgatgcag ttaattgggg agctacatcg 240
gaatcctcaa aaacattgtt ggttcctact ctatccgctg ttagcggtgg tccgggaaaa 300
gatgcttgcc agggagactc taataatgta caattgggag ttgtcatttt ttgggcttgt 360
gccatgtaca agtattattt tttactattg aaagatggta gctgtaagaa aagttacttg 420
ctttgtgggg gataccaagc gttagaaaag ggtaaggagt attaccaatc catcatcagc 480
aaagataaag gcactataca cggtaaagtg acgaataact attcgaccaa atcgatgccc 540
aattataatt ctacactacc ggtacctgcg aaaatcgtaa gtgaaaacaa tggggctaag 600
acaacattat ttgtcaccgg tgacagaatg agtgtgtcat gcaaaaaagt agcagaaaac 660
ttttctcttt gtgcaggcgt ctctcaagga tcatttggaa gtggctgtgc ccgtcctgga 720
aaccctggtg tatatgcaaa tgtcacagca ctccgtggtt ggatacgaaa gactgcagga 780
gtttta 786

Claims (10)

1. a kind of cell-repairing oral liquid, which is characterized in that include following raw material：Bird's nest extracts collagen 10 ~ 30 Part, 1 ~ 5 part of natural ferment, 50 ~ 80 parts of purified water, 1 ~ 10 part of corrigent.
2. cell-repairing oral liquid according to claim 1, which is characterized in that the cell-repairing oral liquid also includes as follows The raw material of parts by weight：2 ~ 10 parts of anemarrhena asphodefoides extract, 2 ~ 5 parts of phellodendron extract, 20 ~ 50 parts of passion fruit extract.
3. cell-repairing oral liquid according to claim 2, which is characterized in that
The preparation method of the anemarrhena asphodefoides extract is：1 ~ 2KG rhizoma ane marrhenaes, are added 8 ~ 12KG water, and 50 ~ 100 DEG C of 4 ~ 8h of extraction are carried It takes liquid to filter, 200 ~ 300g medicinal extract is obtained after filtrate concentration；
The preparation method of the phellodendron extract is：8 ~ 12KG water is added in the Cortex Phellodendri medicinal material of 1 ~ 2KG, and 60 ~ 100 DEG C are extracted 4 ~ 8h, Extracting solution filters, and 200 ~ 300g medicinal extract is obtained after filtrate concentration；
The preparation method of the passion fruit extract is：The pulp of 1 ~ 2KG passion fruits is smashed, is filtered, is obtained after filtrate concentration 100 ~ 200g medicinal extract.
4. cell-repairing oral liquid according to claim 1, which is characterized in that the bird's nest extracts collagen molecules amount and is 500 ~ 180 Gaussian Profile peak area percent is the Gaussian Profile peak area that bird's nest extracts collagen molecules amount 1000 ~ 500 2 ~ 3 times of percentage are 6 ~ 8 times of the Gaussian Profile peak area percent for the polypeptide that molecular weight is 5000 ~ 1000.
5. cell-repairing oral liquid according to claim 4, which is characterized in that point of the bird's nest extraction collagen Sub- measurer has following Gaussian Profile：
。
6. cell-repairing oral liquid according to claim 1, which is characterized in that the preparation of the bird's nest extraction collagen Method is：
Step 1: cleaning：Fresh bird's nest is cleaned up, 1 is pressed with deionized water:20~1:50 mixing, are dipped to bird's nest and have absorbed water Entirely；
Step 2: denaturation：Soak is warming up to 90 ~ 95 DEG C, is kept for 10 ~ 15 minutes, makes protein denaturation；
Step 3: enzymolysis：Soak is cooled to 30 ~ 45 DEG C, recombinant protein is added by 0.05% ~ 0.5% weight of bird's nest dry weight Enzyme adjusts pH to 5 ~ 6.5,4 ~ 6 hours is kept the temperature at 37 ~ 45 DEG C, and PH is nature PH, is not required to control；
Step 4: inactivation：80 ~ 90 DEG C keep the temperature 2 ~ 4 hours；
Step 5: filtering to obtain the yellowish liquid of clarification；
Step 6: concentration, is spray-dried or is freeze-dried to obtain pale yellow powder.
7. cell-repairing oral liquid according to claim 6, which is characterized in that the complete genome of the recombinant protease DNA sequences are：
ACGAATGATGTTGGGGGGGGCGGATACATCGATAAAGCTCCTAGTCAAAGAAAAATTGTTAGCGTTACA
TTAACCGCTCAAGTACACAGGCATATTGCTCATTGTCTTGTTGATAATATATCAGCAAGAGTTGGTAGT
GATTATGATATAGCAATCCGAACTTACGTGGCTATTTTGACATTCTCACAAGATGACGGTGTCAACACT
GGCGGTGATGCAGTTAATTGGGGAGCTACATCGGAATCCTCAAAAACATTGTTGGTTCCTACTCTATCC
GCTGTTAGCGGTGGTCCGGGAAAAGATGCTTGCCAGGGAGACTCTAATAATGTACAATTGGGAGTTGTC
ATTTTTTGGGCTTGTGCCATGTACAAGTATTATTTTTTACTATTGAAAGATGGTAGCTGTAAGAAAAGT
TACTTGCTTTGTGGGGGATACCAAGCGTTAGAAAAGGGTAAGGAGTATTACCAATCCATCATCAGCAAA
GATAAAGGCACTATACACGGTAAAGTGACGAATAACTATTCGACCAAATCGATGCCCAATTATAATTCT
ACACTACCGGTACCTGCGAAAATCGTAAGTGAAAACAATGGGGCTAAGACAACATTATTTGTCACCGGT
GACAGAATGAGTGTGTCATGCAAAAAAGTAGCAGAAAACTTTTCTCTTTGTGCAGGCGTCTCTCAAGGA
TCATTTGGAAGTGGCTGTGCCCGTCCTGGAAACCCTGGTGTATATGCAAATGTCACAGCACTCCGTGGT
TGGATACGAAAGACTGCAGGAGTTTTA。
8. cell-repairing oral liquid according to claim 1, which is characterized in that the corrigent is table sugar or xylitol.
9. the preparation method of cell-repairing oral liquid described in claim 1 ~ 8 any claim, which is characterized in that comprising as follows Preparation process：
（1）Bird's nest is extracted into collagen, hundred natural ferment, corrigent, anemarrhena asphodefoides extract, phellodendron extract, hundred by weight Fragrant berry extract, water are uniformly mixed；
（2）By step（1）Gained mixture liquid filters, and encapsulation, heat treated sterilization to obtain the final product.
10. preparation method according to claim 7, which is characterized in that the filtering uses sieve for 5 ~ 10 mesh.
CN201810127149.4A 2018-02-08 2018-02-08 A kind of cell-repairing oral liquid Pending CN108450952A (en)

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CN1939365A (en) * 2005-09-27 2007-04-04 齐炳显 Cell-repairing oral liquid
CN101497910A (en) * 2008-01-31 2009-08-05 海城市宏大牧业有限公司 Method for preparing enzymolysis oligopeptide protein
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