CN108441554A - 一种筛查遗传性眼科疾病的基因芯片及检测方法 - Google Patents

一种筛查遗传性眼科疾病的基因芯片及检测方法 Download PDF

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CN108441554A
CN108441554A CN201810256622.9A CN201810256622A CN108441554A CN 108441554 A CN108441554 A CN 108441554A CN 201810256622 A CN201810256622 A CN 201810256622A CN 108441554 A CN108441554 A CN 108441554A
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奉珊娜
李莹莹
陈莹
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Beijing Zhong Yin Technology Co Ltd
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Abstract

本发明公开了一种筛查遗传性眼科疾病的基因芯片制备及其使用方法，以及针对致病基因突变位点的检测方法。根据中国人遗传性眼科疾病相关的441个致病基因，设计基因筛查芯片，并对待测样本进行致病基因筛查，对获得的遗传数据进行分析，确定出高度可疑的致病突变。利用降落PCR(touchdown PCR)的方法扩增目标序列，对目标序列进行酶切鉴定或者通过sanger测序的方法进行分析，从而确定该待测样本的致病基因是否发生了基因突变。本发明提供的基因芯片可筛查多种中国人遗传性眼科疾病相关的致病基因，并提供了针对目标基因突变位点的检测方法，大大降低了眼病患者的检测成本，提高了检测效率。

Description

一种筛查遗传性眼科疾病的基因芯片及检测方法
本发明涉及基因芯片及基因检测技术领域，具体涉及一种筛查遗传性眼科疾病的基因芯片制备及其使用方法，以及针对致病基因突变位点的检测方法。
背景技术：
近些年，随着医疗技术的进步和人们生活水平的提高，由环境所致的常见致盲眼病(如沙眼等)基本得以控制，部分致盲眼病(如白内障等)通过手术可以治愈，而与遗传有关的遗传性眼科疾病逐渐成为难治或不可治愈双眼盲的主要原因。目前已报道的遗传性眼科疾病达到600多种，按照解剖学部位包括角膜病、眼睑病、青光眼、泪器病、玻璃体病、晶状体病、视网膜病、色素膜病、眼肌病、视神经病、眼屈光不正和综合征等，这也成为我国主要的致盲原因之一，25岁以下青少年盲人中，遗传性眼科疾病致盲者占67.7％。遗传性眼科疾病特别是遗传性眼底病具有显著的临床异质性，复杂多样且交叉重叠的临床表型给临床诊断带来了一定的困难。
大多数遗传性眼科疾病是否发病，主要由遗传因素决定，可通过基因检测确定病因，为疾病的诊疗和预防提供科学依据。近年来，很多与遗传性眼科疾病相关的致病基因或致病突变位点被相继报道，这些已知的致病突变位点可作为基因标记，用于遗传性眼科疾病的临床基因诊断、症状前诊断和产前诊断。基因诊断是伴随着遗传学和细胞分子生物学理论和技术迅速发展而产生的一种新型诊断技术，遗传性眼科疾病的基因诊断可以指导临床治疗，包括转基因治疗。眼科遗传病高效、准确的基因检测可通过基因芯片以及后续的测序分析及数据解读发现致病基因，辅助眼科疾病的临床诊断。
基因芯片技术是随着人类基因组计划的进展而开始发展起来的分子生物学技术，它是融合了微电子学、生物学、物理学、化学、计算机科学为一体的高度交叉综合的技术，利用微电子芯片技术的集约化和平行化处理原理，将大量具有生物学意义的DNA、cDNA或寡核苷酸探针按一定顺序密集排列并固定于指甲大小的玻片、陶瓷、尼龙膜等固相载体表面，形成致密、有序的分子点阵，并利用微量反应体积，一次性杂交就可以对多个基因的表达水平、突变和多态性进行特异、快速和精确的检测。由于基因芯片能够快速准确的得出生物样本中大量的遗传和基因信息，因此其在基因表达、基因突变、基因多态性研究和基因诊断等领域中获得了广泛应用。
虽然目前有一些能够筛查多种遗传性眼科疾病的基因芯片出现，但是也只是一种广泛筛查的基因芯片，中国人种由于其独特的遗传结构，其遗传性眼科疾病相关基因的突变位点和核苷酸多态性上与其他人种都有所不同，因此，亟需一种能够针对中国患者遗传性眼科疾病基因特点的基因诊断芯片，这不仅可以大大降低基因检测的数目，而且降低了检测成本，提高了检测效率。
发明内容：
为了克服现有技术中存在的上述问题，本发明通过建立中国患者常见眼科遗传病基因的组合，并根据中国患者基因突变的特性设计富集方案，达到高效靶向富集重要眼科遗传病基因的目的，将2万多个基因的测序通量降低到400多个基因，大大降低测序成本。设计的遗传性眼病基因筛查芯片涵盖了对中国眼科遗传病患者诊断治疗有重要意义的全部基因，可有效捕获多种常见遗传性眼科疾病(包括RP、Leber先天性黑矇、Stargardt病、锥杆细胞营养不良、先天性静止性夜盲、Usher综合征和Bardet Biedl综合征等)的441个致病基因。利用设计的基因筛查芯片对待测样本进行致病基因筛查，对获得的遗传数据进行生物信息学分析，确定出高度可疑的致病突变。
对于具体致病突变的确认，本发明利用降落PCR(touchdown PCR)的方法扩增目标序列，对目标序列进行酶切鉴定或/和通过sanger测序的方法进行分析，从而确定该待测样本的致病基因是否发生了基因突变。与常规的PCR方法相比，降落PCR是一种简单有效的方法，首先在较高的温度下扩增，温度的升高提高了PCR扩增的特异性，但也提高了引物结合的难度，降低了扩增的效率，因此一开始先用高温扩增，保证扩增的严谨性，待目的基因的丰度上升后，降低扩增的温度，提高扩增的效率，此时非特异的位点由于丰度低，无法和特异位点竞争，从而大幅度提高了PCR的特异性和效率。采用降落PCR(touchdown PCR)扩增并对扩增产物进行酶切鉴定或/和sanger测序的方法，对目标基因中的突变位点进行检测，该方法与常规的基因检测方法相比，具有灵敏度高、结果准确、操作简单、方便快捷，价格低廉等优点。
本发明具体提供了一种基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，所述应用包括以下步骤：
1)使用基因筛查芯片对致病基因进行筛查，确定出高度可疑的致病突变；
2)通过降落PCR(touchdown PCR)扩增方法，对致病基因的致病突变进行检测。
所述基因筛查芯片包括基因筛查芯片载体以及固定在所述载体上的寡核苷酸探针，所述寡核苷酸探针根据中国人特异的遗传性眼科疾病相关的基因设计；所述中国人特异的遗传性眼科疾病相关基因包括441个相关基因；所述寡核苷酸探针的序列如SEQ IDNO.1～496所示。
所述步骤2)还包括提取待测样本的基因组DNA，设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增致病突变所在的核苷酸序列，对扩增产物进行酶切鉴定或/和sanger测序的方法进行分析，从而确定致病基因是否发生了突变。
本发明还提供了一种遗传性眼科疾病的基因筛查芯片，其特征在于：所述基因筛查芯片包括基因筛查芯片载体以及固定在所述载体上的寡核苷酸探针，所述寡核苷酸探针根据中国人特异的遗传性眼科疾病相关的基因设计；所述中国人特异的遗传性眼科疾病相关基因包括441个相关基因；所述寡核苷酸探针的序列如SEQ ID NO.1～496所示。
本发明还提供一种诊断试剂盒，所述诊断试剂盒包括所述的遗传性眼科疾病的基因筛查芯片。
如下表1所示，本发明共涉及441个相关基因的496个探针序列，其中有些基因一个基因设计了多个探针序列，分别针对不同的区域。例如基因ARL13B，共选取了两个基因突变位点区域，设计了两个探针，其核苷酸序列分别如SEQ ID NO:29和30所示。
表1 441个相关基因以及对应的496个探针序列
附图说明：
附图1为sanger测序结果示意图；
附图2为酶切鉴定分析示意图。
具体实施方式：
本发明所述遗传性眼科疾病的基因筛查芯片的制备方法包括以下步骤：
1、根据人类基因组数据库、人类基因突变数据库(Human genes mutationdatabases，HGMD)(http：//www.hgmd.cf.ac.uk/ac/index.php)以及Pubmed、CNKI、万方数据库近年所发表的中英文文献，对遗传性眼科疾病相关基因的突变位点进行统计，以中国人群报道次数较多的位点为主，并筛选出相关的致病基因和突变位点；
2、根据致病基因的核苷酸序列，在其外显子和相邻内含子部分区域上设计相应的探针序列，所述致病基因与探针序列的对应关系参见表1；
3、利用原位合成技术，在载体上大量合成设计好的探针，并利用PCR的方法扩增出大量的带有生物素标记的探针，构成为遗传性眼科疾病的基因筛查芯片。
本发明所述遗传性眼科疾病的基因筛查芯片的使用方法包括以下步骤：
1、抽取遗传性眼科疾病患者的血样，提取DNA，打断，扩增，构建遗传性眼科疾病患者的基因组数据库；
2、使用所述的遗传性眼科疾病的基因筛查芯片进行致病基因筛查，该芯片可有效杂交捕获相关的致病基因外显子及相邻内含子部分区域，并进行富集；
3、捕获后的DNA经扩增后使用Illumina Solexa HiSeq 2000测序平台进行测序，在UCSC数据库上获取参考基因组序列，使用BWA工具将短序列排列组装成完整的参考基因组，并将其转换成BAM格式，然后对序列进行重新排列，减少插入或缺失错误。随后使用软件Samtools和Picard调整短序列顺序，去掉测序冗杂信息得到最终的BAM文件，使用生物信息学软件Samtools mpileup and bcftools，查找单核苷酸多态性(SNPs)及插入或缺失突变(indels)，使用CoNIFER分析拷贝数变异(CNVs)，使用ANNOVAR对SNPs和indels进行功能注释。应用生物学数据库：1000Genomes，ESP 6500，ExAC以及SNP database对SNPs和indels进行分析，排除多态性位点，最后确认高度可疑的致病突变；
4、使用PolyPhen-2、SIFT、MutationTaster等在线软件对可疑的致病突变进行分析，预测突变对编码蛋白质的功能及结构是否产生重要影响；
5、围绕上述致病突变设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增致病突变所在核苷酸序列，对扩增产物进行酶切鉴定或/和sanger测序的方法进行分析，从而确定致病基因是否发生了突变。
下面将通过具体的案例做进一步的说明。
案例1：
先证者1，临床诊断为视锥-视杆细胞营养障碍(CORD)，是视网膜色素变性的一种变异型，又称为反向视网膜色素变性。临床表现为缓慢进展的双眼视力下降、畏光、色觉差。日间实力比黄昏视力差，晚期伴有周边视野丧失和夜盲。
从先证者1外周血中提取基因组DNA，构建基因组文库，然后使用所述的遗传性眼科疾病的基因筛查芯片进行致病基因筛查，经过数据分析统计后发现，高度可疑的致病基因和致病突变如下：
致病基因 致病突变
CYP4V2 c.237G>T；p.E79D
CYP4V2 c.367A>G；p.M123V
SLC7A14 c.1391G>T；p.C464F
FBN1 c.3463+3A>G
其中c.237G>T表示核苷酸位点第237位发生突变，由G变为T；p.E79D表示氨基酸位点第79位发生突变，由E变为D。以下类推……。
案例2：
先证者2，临床诊断为双中心性RP(色素性)，视网膜色素变性(retinitispigmentosa)是一组以感光细胞和色素上皮细胞进行性选择性丧失为特点的遗传致盲眼病，世界范围内发病率为1/3000，是致盲的主要病因之一，目前无有效疗法。该病早期症状为夜盲，随病程发展，中心视力逐渐减退，最终可完全失明。
从先证者2外周血中提取基因组DNA，构建基因组文库，然后使用所述的遗传性眼科疾病的基因筛查芯片进行致病基因筛查，经过数据分析统计后发现，高度可疑的致病基因和致病突变如下：
随后利用所述的遗传性眼科疾病的基因筛查芯片对先证者2妹妹的血样进行筛查发现，先证者2妹妹携带致病突变(ABCA4c.6095A>G；p.H2032R)。
案例3：
先证者3，也被临床诊断为视网膜色素变性(retinitis pigmentosa)。从先证者3外周血中提取基因组DNA，构建基因组文库，然后使用所述的遗传性眼科疾病的基因筛查芯片进行致病基因筛查，经过数据分析统计后发现，高度可疑的致病基因和致病突变如下：
致病基因 致病突变
PDE6B c.1467+1G>C
PDE6B c.1655G>A；p.R522Q
USH2A c.5608C>T；p.R1870W
随后利用所述的遗传性眼科疾病的基因筛查芯片对先证者3兄弟姐妹的血样进行筛查发现，先证者3的哥哥携带致病突变(PDE6B c.1467+1G>C)；先证者3的大姐携带致病突变(PDE6B c.1655G>A；p.R522Q和USH2Ac.5608C>T；p.R1870W)；先证者3的二姐携带致病突变(USH2A c.5608C>T；p.R1870W)。
案例4：
先证者4，临床诊断为Leber先天性黒朦(Leber congenital amaurosis，LCA)是一种导致儿童双眼先天盲的遗传性视网膜疾病，占儿童先天性双眼盲的10-20％，占遗传性视网膜变性类疾病的5％。眼底检查早期多为正常，随着病变进行性进展，数年后可见眼底椒盐样色素沉着、骨细胞样色素、视网膜血管狭窄、广泛视网膜色素上皮和脉络膜萎缩，视网膜电图表现为a、b波平坦，甚至消失。
从先证者4外周血中提取基因组DNA，构建基因组文库，然后使用所述的遗传性眼科疾病的基因筛查芯片进行致病基因筛查，经过数据分析统计后发现，高度可疑的致病基因和致病突变如下：
随后利用所述的遗传性眼科疾病的基因筛查芯片对先证者4的父母的血样进行筛查发现，先证者4的父亲携带致病突变(CEP290c.6012-2A>G和RGS9BP c.607dupG；p.C206Lfs.)；先证者4的母亲携带致病突变(CEP290c.1834C>A；p.L612I和ERCC6c.2703C>A；p.Y901*)。
上述案例足以证明了本发明所述的遗传性眼科疾病的基因筛查芯片可以有效并且准确的诊断出遗传性眼科疾病患者的遗传信息，为眼科疾病的临床诊断提供了一种快速和准确的检测工具，大大降低了中国人眼科疾病患者的检测成本，提高了检测效率。
为了进一步验证通过遗传性眼科疾病的基因筛查芯片确定出的致病基因的突变位点，我们利用了降落PCR(touchdown PCR)的方法扩增致病突变所在核苷酸序列，对扩增产物进行酶切鉴定或/和sanger测序的方法进行分析，从而确定致病基因是否发生了该突变。
为了更好的描述该验证过程，我们利用所述的遗传性眼科疾病的基因筛查芯片对角膜营养不良患者进行了筛查，并发现了TGFBI基因的致病突变(c.371G>A；p.R124H)，通过降落PCR(touchdown PCR)扩增方法，对该致病基因的突变位点进行了检测。本领域技术人员应当知晓，该检测或验证过程只是举例说明，而非穷举，其他致病基因及其突变位点的检测过程均与之类似。
首先，提取患者血样的基因组DNA，在TGFBI基因第4个exon上下游设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增目的序列。
所述降落PCR的反应体系为：DNA模板1μl、5×PrimeSTAR Buffer 5μl、1×dNTPMixture 4μl、Forward Primer 0.5μl、Reward Primer 0.5μl、PrimeSTAR HS DNAPolymerase 0.5μl、ddH2O 13.5μl，同时采用阴性对照，所述阴性对照的反应体系与上述反应体系相同，仅仅是加入的模板不同。
所述降落PCR的反应条件为：(1)95℃5min；(2)95℃30sec；(3)64℃30sectemperature-0.5℃；(4)72℃40sec go to(2)，cycle 14；(5)95℃5min；(6)57℃30sec；(7)72℃40sec go to(5)，cycle 21；(8)72℃10min；(9)4℃59min；(10)4℃59min go to(9)，cycle 99。
所述引物分为正向引物和反向引物，引物序列如下：Forward Primer：TGCTCTCTGTCAGAGAAGGGA；Reward Primer：GCCTCTCTTCTGCCAAGGAT。
利用sanger测序进行分析，将扩增产物进行测序并用分析软件Sequencher5.1Demo进行分析，如果待测样本TGFBI基因序列峰形与标准序列峰形一致则待测样本中TGFBI基因未发生突变；如果待测样本TGFBI基因序列峰形与标准序列峰形比对不一致且出现双峰则样本为杂合突变；如果待测样本TGFBI基因序列峰形与标准序列峰形比对不一致且出现单峰则待测样本为纯合突变。具体到本案例，其sanger测序结果为杂合突变，参见图1。
此外，还可以对扩增产物进行酶切鉴定，采用的内切酶为Acil酶，扩增产物中若两条DNA链均未发生R124H突变则出现两条分别151bp、342bp条带，若有一条链突变则出现三条分别为493bp、151bp、342bp；若两条链均突变则出现一条493bp条带，参见图2。
为了增加检测的准确性，还可以同时对扩增产物进行酶切鉴定和通过sanger测序的方法进行分析。
以上所述仅为本发明优选的实施例，并不用于限制本发明，对于本领域技术人员来说，本发明可以有各种更改和变化。凡是在本发明的精神和原则之内，所作的任何修改、等同替换或改进等，均应包含在本发明的保护范围之内。
<110> 北京中因科技有限公司
<120> 一种筛查遗传性眼科疾病的基因芯片及检测方法
<160> 496
<170> SIPOSequenceListing 1.0
<210> 1
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 1
ggggccgcgc agcggcctct cgtctactgc caccatgacc gccaacggca cagccgaggc 60
ggtgcagatc cagttcggcc tcatcaactg cggcaacaag tacctgacgg ccgaggcgtt 120
<210> 2
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 2
aatatgttac taattaatct gtggacagag taatgctaat actaggaggt ctctgctgac 60
ctgattgatg cttcattgga agttcagttc tttctgaaac gtaaataagc ttttcttctt 120
<210> 3
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 3
agagagtgcg acccctcgcc cggcccggcg agccccgggc gtgaaccgag ctgagggagg 60
atggcagcct ctggggtgga gaagagcagc aagaagaaga ccgagaagaa acttgctgct 120
<210> 4
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 4
cccacatata ctcctctctc ggcgtctcta tcctgctgct ttctctgact gcatcttcct 60
ctgttccttg gtcagtgttt gagaagagaa tgttgaacaa gtcacatcct tgtcactctg 120
<210> 5
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 5
ataattatta gttcatagtg tgaagtagat tattataaat gttttctttt tagtgtgaaa 60
gaaaatgtct ttatttaaag cccgtgattg gtggtctact attctgggag ataaagaaga 120
<210> 6
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 6
aaaagccatc aaaaaactta acagaaccaa atcaaaaaga agtatatttt atgctaaagt 60
tactttctgt ccaggtcgaa acattgttcc actttgattt tttctcctct ttggattttg 120
<210> 7
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 7
gaccttggcg gagcggcgga ggcgcccagc ggaggtgaaa gtattggcgg aaaggaaaat 60
acagcggaaa aatgcagagc tggagtcgtg tgtactgctc cttggccaag gtgagggccg 120
<210> 8
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 8
tgtggttttc ttctgtgaca tttcttttta taactcctcc atctcaaagt gtgtattaaa 60
gctgtttgcc atggatgtgt gttcaaagat tcttgacagg cttggtttca acagttctac 120
<210> 9
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 9
tatcacacac atgttcagag gtgtattgcc tgggaagaaa aaaacaaaat ataattccca 60
attatggttt aattgtactc tgtgtagtat atataatgcc ttctgcttta attcgctcca 120
<210> 10
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 10
tcagttatgg agggaggctg tgcccaaaag tggacactgg ggtgcatccc ctccaccacc 60
tcggcctcca ccgctgtcct cagtacagcc gcttttcgta actgtgggga caaggcaaga 120
<210> 11
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 11
gaaacttact taaaagtaaa atttaattct agctgttgca aacaggccaa tattgggtcc 60
tcagttgggg ccaacttggg tagacgagac agtagaaact tctgttttct gggagctgca 120
<210> 12
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 12
tctgaaaaga ggaagaacat ttatttgcct aagtgagaca aagtctttac ttccagggtt 60
tgccttgcag gtgaccactg ctgagggagc gggggtttga gtggctggcg gggccgccac 120
<210> 13
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 13
gcaaatctgc gaggcgaggc cgatcagctg tggaagttct gcacggtgct ctgtttctgg 60
gagagccgga ggagctcctc gcggattgct ttgatgagag aggccgagga gtggccaggc 120
<210> 14
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 14
acatgaagac catggataaa ttatattttt ttctctttcc gcctctacta acctagcaaa 60
tctctagaag atgacggtgt tctttaaaac gcttcgaaat cactggaaga aaactacagc 120
<210> 15
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 15
tgactgctca cagatcccgg ggctcaccta gtagagcacg ccctcaaaat ccagctgcag 60
gtagcggagc aggcgcggtg gcagggggag gcggggcagc gcttggggca ggctgccctc 120
<210> 16
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 16
acagcggcgg cgggggcggg gctggcctca cccggaatga aaacaaacgg cggccgctgc 60
cgcatccggg cactctgctg gtcgcggcgg gagtggcgtg gcgcaggtga ggacacggcg 120
<210> 17
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 17
aaagataaca gtttcaagta cattgtgata cacgttttga caggaataga tatgcattta 60
gtcatgaggg aggctggcag caaccaccat gcatacattt tgcacaaact cttggctccg 120
<210> 18
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 18
aaagtactta cctcactttg tggctcctgg ataactttat agagagatga aactaaagaa 60
ctcttgtctt tcaaatttgt ggcataattt ggtaaagatg tttctggtaa tgtctaaaca 120
<210> 19
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 19
ttaaatttct ttcatacagg taccaggtac ttgcttttgc aacagatgca gataaaaggc 60
aggagactga acagcaaaaa cttggttctg gaaaaagact agttgtaagg ccttttttta 120
<210> 20
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 20
ccacaggagg gtcagagcgg catcttccct tcagggccct cgcccgggca ccccccggcc 60
aatggaccac agctgcaccc ggttcatcca ccgccgggga ccacccactc ggacccgagc 120
<210> 21
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 21
ttggcgttca gactcccgta gaggtcatct gtgtccaggc ccacccgggc gccggctcag 60
cggtagtgcc gaaggtagat gagcttctgg ggagggaact tctcccggca gaggggacac 120
<210> 22
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 22
acagacaggc cccagctggg tgccgcagga aggacgtcac cctcaagccc tcactggtag 60
atgaccttca cgcaaaatgc ctcttcgttt ttgtcctcat ggtcattgat gtagatcagg 120
<210> 23
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 23
cctttgtaga caacaaggga ggtgtcacag ttttccattt agatcaacaa cttcaagttc 60
ttaccatgga aaattccgag aagactgaag tggttctcct tgcttgtggt tcattcaatc 120
<210> 24
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 24
cgggagggcg agagcgaggg cgcctgggag aaacctcgcc cagcggcggt tgattagtca 60
ggcctcagaa agatggcgtc ctcggagcag gcagagcagc cgagccaggt aaggggagtg 120
<210> 25
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 25
cacttttaga gtttacttca accacgtgga gcttccatgg cggcctctca ggtcctgggg 60
gagaagatta acatcctgtc gggagagact gtcaaagctg gggacaggga cccgctgggg 120
<210> 26
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 26
gggttcgctc acgttagctt ctcttgcagc gcaatgtccc tgctcttctc tcgatgcaac 60
tctatcgtca cagtcaagaa aaataagaga cacatggctg aggtgaatgc atccccactt 120
<210> 27
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 27
gagaaaagtg gtaaagaatc tacctcactg ggaatgtcat cattaccaac ttcagatggg 60
tttaaccatc ccgcccgttc ttcaggacag agtcctgatg ttggtaatcc tatgagtctt 120
<210> 28
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 28
aactagaaat aaataaagtc cccagtggcc cagcatggca cagcagggag gccactctgt 60
ttcttcaagt attcttggcc gatggggctc caggccctgt cgaggctcag atggggatcc 120
<210> 29
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 29
ctctccactt ttaggcacag tctttgttct ttatcgccag gcccgattca ggagcttcac 60
ctgtgccagt ctcttagtga tcatggtggc aaaaaccagg ccaaagagga cgctgctgat 120
<210> 30
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 30
tgaatgggcc acgggggcat tgcctgatag atctgttgtg ggatgtgttt ttttttccag 60
aaccccagag atgaaaggcc tcctcccact ggcttggttc ctggcttgta gtaagtgctg 120
<210> 31
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 31
caagattacc tggctggtgt ttgcttgttc tggagtgatc ttctgactgg aaaagaacta 60
tgtcatggat caaggaagga gagctgtcac tttgggagcg gttctgtgcc aacatcataa 120
<210> 32
<211> 3
<212> DNA
<213> Artificial Sequence
<400> 32
<210> 33
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 33
ttgcttccct cctaggcctg aggcccagcc agcgcccaat ggatgacaaa aagaagaaac 60
ggagtcccaa gccctgcctg gcccagccag cccaggcccc aggcacacta cggagggtcc 120
<210> 34
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 34
gaggccaggg cagcaggacc ccccccgcgg gttatgtggg gcagagcaag aatcctgaaa 60
aggaggagtg gatgtactcc gtggagtaga ggccgttggc ctggtccgac ggcatctgca 120
<210> 35
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 35
gctctgtctg ggcctgatgc tcaaggcccc ttgatggatc caggctctgt aaggcgggca 60
gaggcatagg ccgaagctcc aaggcaggcg gcaggttcac agaagcccgg cagccccacg 120
<210> 36
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 36
gccgtgtcca tataaagcgg gtcggcggcc cctgcggtcc cggagccgcg cgggcagggg 60
ctgccgcagc cgatggcggg gcgcagcgac atggatccgc ccgccgcgtt ctctggcttc 120
<210> 37
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 37
taccccaccc catcccctgc cccgccgggt acctacaagc tcggttcctt tctcaactcc 60
cccagttcct tgatctccac cttcttgtac ttccccgact ttctccggtt ggtgatcacc 120
<210> 38
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 38
gagagccggt aggagggagt gtgaaggtgt ccgcggcgtc gtcgacggcg gcgccggcca 60
tggaggagac gcagccgccg ccgcagccta agctgcccct gtgcgacagc ctcatgatct 120
<210> 39
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 39
ttttctcagt tttgctacca aaaacatatc ttgctggagt atgctcaaga ttttttgaac 60
agtccatgaa aggtgacagg gatgttaatc tccacttcag cccgggcaac ttcacttaag 120
<210> 40
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 40
ggctatttaa aaggtttcct ggttctatga agctgcttcg gccattagta gagaaaatga 60
agtttctgta atggaggcag gctttttaag tagtgacgtg acttccacca tgccagctcc 120
<210> 41
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 41
catttggatg accatatggg cacaggctcc tgcgcctcca gctgcccaga gttcctttct 60
ggctgcagga acgagcggtg tgaaagatca gtcctgtgga aggagatcac atggactcgc 120
<210> 42
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 42
caccaaggta tattttctgt tgatttgata tgttctttgt cttaatctta gccaagaaaa 60
caaacaggac caacttcaaa tccgaacttc tgattctgat caccaaagtc attgatcatg 120
<210> 43
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 43
acttatacct gaggaatggt gaggattaga agaggccgca gtccttgagg ttttctttga 60
tgataatatc tgtaactgca tcaaacacaa atttgacatt ctgtgtatct gtagcacagg 120
<210> 44
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 44
tgtctctaca ctggaggtgg actctctcac gcataaacct gcatgttgtt gtgtggtggc 60
tcagacatgt gtgtcccagg tccccgctga cctcctccag cacccccagg ggtagggctg 120
<210> 45
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 45
gctcttgcct tctccctcat ttcttcaggt gggtgagaaa tgggcgactg gagtttcctg 60
gggaacatct tggaggaggt gaatgagcac tccaccgtca tcggcagagt ctggctcacc 120
<210> 46
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 46
gtgtagtatt gagtcctgtt tgagctattg ttctcttttt cctgaaaaat ggcactgtca 60
aagagggagc tggatgagct gaaaccatgg atagagaaga cagtgaagag ggtcctgggt 120
<210> 47
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 47
caccctccac gcccagatgc ctgcgtagtt tttgtgacca gtccgctcct gcctccccct 60
ggggcagtag agggggagcg atggagaact ggactggcag gtgagagaag gggccacggg 120
<210> 48
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 48
gcccgggtgc ctgtttcccc agagctccct ggtgacagtc tgtggctgag catggccctc 60
ccagccctgg gcctggaccc ctggagcctc ctgggccttt tcctcttcca actgcttcag 120
<210> 49
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 49
ccagatgttc cccatagctg ggactcagag aggaaaacgt gttgtgtttc acatgatcag 60
acactgttca ccactggcac ctggtgggcc cgaaagattg agggcatcca ggtcaaagtt 120
<210> 50
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 50
cgatgaccat cacctttggg cctttggcgg actccacccc taacccccga tcccccgccc 60
ggcccgctaa ccgctatttc ttatccttgc gagactcccc cagccccttg gccttcttct 120
<210> 51
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 51
gccgtcccag atattaatca cggagttcca gggagaagga acttgtgaaa tgggggagcc 60
ggctggggtt gccggcacca tggagtcacc ttttagcccg ggactctttc acaggctgga 120
<210> 52
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 52
tccaggcagt tgctgtgcag agttcttgct ataagaagaa gcccttcagc agtatattat 60
gaagtggtta cttgagaact tgattgtgga tcttccagct ttctacattt cttcataaca 120
<210> 53
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 53
gccattgcct gtacagcttg gaggcttttc acatcttgga gagcttgatg tcataagtga 60
ccatgttcaa gttgtcccag gcaaagagct tcttctccag ggggttgtag tcaatcatgc 120
<210> 54
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 54
ggggaaaaaa aagaaaatga tttcctggga agttgtccat acagtattcc tgtttgctct 60
tctttattct tccctagctc aagatgcgag cccccagtca gagatcagag ctgaggaaat 120
<210> 55
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 55
aggagaactg gacgttgtga acagagttag ctggtaaatg tcctcttaaa agatccaaaa 60
aatgagactt ctagcaaaga ttatttgcct tatgttatgg gctatttgtg tagcagaagg 120
<210> 56
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 56
cggactggga ccagaccacc agcaacacac cagaggatgt tctctaaata agaccatggc 60
acttaagaac attaactacc ttctcatctt ctacctcagt ttctcactgc ttatctacat 120
<210> 57
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 57
aagttatttt taaaagcact tgggaagttc ctcctccacc ccgcaggtgg gaaggctcag 60
agaagggtgt catcagtaca gccgccttct aggccgtaac ggaattcctg aaggttggag 120
<210> 58
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 58
aaggctccgc ttctgggcag ggaagcggcc ggggtcagtc ggctttgggc gcccggaatt 60
cgggcatgct ccaggaccgc agtggcggcg gtgtgtcccg ctccacgtcc tcggagatgg 120
<210> 59
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 59
ttacatcctg tacacagctc tgtgtctctc tacctggcta gcgcatgccc aggatctgtc 60
tgcttttcag ttggtaattt ttctcaatat ctgacagggc ttgagcccgc agctgggcag 120
<210> 60
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 60
tggcgccggg gagcctggga tatggcgcgg ccagacgatg aggagggggc ggcggtggcg 60
cccggacacc cgctcgcgaa aggatacctc ccgttgccga ggggcgcgcc cgttgggaag 120
<210> 61
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 61
tgcagacctt gcattccagg gttacgtctt ctgggtttcc atcctttaca ggtgggtgtc 60
tgtgaatgtg gtgcgttcct tagtcactga gctgaaatcc ttgatggcgt tcatcaggtc 120
<210> 62
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 62
atgttataat cataatttta gactatttcc agtaggtaag tgtcataaag aaggaagaga 60
tatggcttcc acagcttcaa tgaggtgtaa ggctagacca tattgcccat gtttttctgg 120
<210> 63
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 63
gggagaaagc tggacatttc cccacgtaac tcccagctct gggcctagag tgcgtgcatg 60
gcgaagtccc cggagaactc taccctggag gagattctgg ggcagtatca acggagtctc 120
<210> 64
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 64
agcggccgag cgcggggaaa tgaaggcagc cgccgggcag gaaagcgagg gccccgccgt 60
ggggccgccg cagccgctgg gaaaggagct gcaggacggg gccgagagca atgggggtgg 120
<210> 65
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 65
cccgcgctgc tggtgccgcc gccttctgcc ggaccgggcc cctcgccgcc cgcgcgtctg 60
ttcagcgtcg acagcctggt gaacctgcag ccggagctag cggggctggg cgcccccgag 120
<210> 66
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 66
ggaatctaga cttgtgagcg gcggtggtgc ctgcagtgac actggtgcgt gcactcctgc 60
gcggtctttc ttagcgcagc aacggcacat caccaacacc agaagaaatc tctctgttaa 120
<210> 67
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 67
tcccagcccc accatggagc tgcgctgtgg gggattgctg ttcagttctc gctttgattc 60
agggaatcta gcccacgtgg agaaggtgga atctttgtcc agtgatgggg aaggggtagg 120
<210> 68
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 68
gtccgtctgt ataaaacatg gggaagaagg acctagttca ggatgagtct gtgtggacag 60
cccggctgcc agcagtcccc agggctggtg ggccccgaga gctcaaaaca gagggtgggc 120
<210> 69
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 69
tgtggccccc tcgtcagcct gtcaggacac ctcctcttgc tggggctgcg cctctggctg 60
ggatttgtcc tggatgtggc cggtccgagg ctccggttgc agcccgtggc ccagcacaca 120
<210> 70
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 70
tgaatatttc taaaatttca gcttgcctct tgcttcttat tggcaagttt ccttggcttg 60
taaattttgg acagcactat caaggagctc catggactct ctgagagtga cattgacttt 120
<210> 71
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 71
tcaaaataac atgtagacac agaatcacaa atatatacaa aacaaagtat cgcctggtta 60
tttcaagaag agttttccct caattttctt agctgctgtg cataaaattc aaagctttcc 120
<210> 72
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 72
cggcggcggc ggcggcggct ccagggcccc cccggaagag ttgtccatgt tccagctgcc 60
caccctcaac ttctcgccgg agcaggtggc cagcgtctgt gagacgctgg aggagacggg 120
<210> 73
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 73
tgaggaggtt tcgacatggc ggtgcagccg aaggagacgc tgcagttgga gagcgcggcc 60
gaggtcggct tcgtgcgctt ctttcagggc atgccggaga agccgaccac cacagtgcgc 120
<210> 74
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 74
tggctgactt aaatgcctgt ggttgactct tagaaaagac taaaatgaaa atggccccac 60
tattattgtc aatcttaaca cagagcttgt gcggaaggtc cctatactgc tgactgtcag 120
<210> 75
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 75
ggacagtcag gggtaggagc gggagccgag aggaggcgga ggagatggcg tcccagccgc 60
cacctccccc caaaccctgg gagacccgcc gaattccggg agccggaccg ggaccaggac 120
<210> 76
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 76
ctgacgctgc aaacaacagc aagggcatag agaggagacc ttgatgattc agtgtgattc 60
ttcaacagat ttctcttctt cactttcctc attggcttca tctttttcct tgtaagaatc 120
<210> 77
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 77
taagtctgta tcaggccatg aaaagtttag atatgaagaa ggcagttttc tttttttctt 60
aatgtttaca ccagaccaaa gtgaatcatt ttgagagaac caccatctct gatttcctgc 120
<210> 78
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 78
ataacaccta gtttgagtca acctggttaa gtacaaatat gagaaggctt ctcattcagg 60
tccatgcttg cctactcctc tgtccactgc tttcgtgaag acaagatgaa gttcacagtg 120
<210> 79
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 79
gaggaggaag aggaggaggc tgcagcggcg gcggcggcga acgtggacga cgtagtggtc 60
gtggaggagg tggaggaaga ggcggggcgg gagttggact ccgactctca ctacgggccc 120
<210> 80
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 80
aatacacaca gaaagagatc acagactccc taccttagaa gaagggaggt ggtagatgaa 60
atgaactgta tgaagagcca ctagcctggc ccacacacag aagaaggact ggcccgtctt 120
<210> 81
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 81
tctgcaccca acatctggcc cccttcagaa ctctgagtgg acaggatcag gatttgactc 60
aggaggatta gaatgtgaag aatccgtgtt tgagggattc agttctccaa ctgcctcaaa 120
<210> 82
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 82
ctaattcagg ctcaactctc ctttacccaa aagtaaatgc ctcaggactc aatctgaatc 60
actgtctgtc tcagcttctt tcacatccac gctgaatttg tactcctggt cacatcccat 120
<210> 83
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 83
cccgcgggcg cctcctcctg gcggcgccgg tgctgctggt gctgctgtgg gcgctggggg 60
cccggggcca gggcagcccc cagcagggca cgatcgtggg catgaggctg gcgagctgca 120
<210> 84
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 84
tgatgacgtg tctgctttgt gttcacattt tagcaatcat ctggggggct aaatgtgaca 60
aaccgagaag atggccaaga tcaacaccca atactcccac ccctccagga cccacctcaa 120
<210> 85
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 85
agaggctgcc actgtcacac tgcattcttt ctcatttcac ccaagaagtc ataggtctgc 60
tctgaaaggt caaaaggttc atgaactccg tctggtgaca gcagagcttg gagggcgccc 120
<210> 86
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 86
ggttcgggac gtccatctgt ccatccgtcc ggagagaaat tacagatccg cagccccggg 60
atggggccgg ccccgctgcc gctgctgctg ggcctcttcc tccccgcgct ctggcgtaga 120
<210> 87
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 87
ttgtcttctc ttttaggatt gccacccagg acgatgagcg gctgagatgg agacgtctgc 60
ctcagccact gcctccgaga agcaagaagc caaaagtggg atcctggagg ccgctggctt 120
<210> 88
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 88
ggtgttatgc ctggggatgt ggtttctccg agcagcagtg atgtttggag attaatactg 60
aaagctgcgc tttgtctgga tatcatcaag aatctgctgc agctcctcat cttcaaaccg 120
<210> 89
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 89
ggctccgggg gcggggacgc gccccgttct ctttcgctct tcttcccggc agccttagcg 60
ccaggcccgg cgctcctcaa gatggctgcc gacagtgagg tgatttcttt gctccctact 120
<210> 90
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 90
gttagatttc tttcatttcc tgttaaacca acacctaagt taaaattatt ttcaaggtct 60
taaagacgca cgggccttat caagtatatc ctttctgatt tttggataag ttggatgtgc 120
<210> 91
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 91
gtgtgtttct aattattccc taaatagctg gtatagctat acttcagagt cttctttaac 60
aagatttgcg gtgtctttaa aagtgtcttc tgttgcagaa taggttggag ttgggtctct 120
<210> 92
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 92
cagagagacg cagctaggcc tgcacggctg tggagagatc ctgccacggg ccttgttcac 60
catgtcggtg ctggatgcgc tttgggagga tcgggatgtc cgtttcgacc tgtccgcgca 120
<210> 93
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 93
tttttatgtg ctactgaaag attttttaag ttgctccaga tttactttct ttctgctatt 60
atctttaaat actgcaaagc attgtgagct gcatcacttt gtgcattgcc acaggagata 120
<210> 94
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 94
gacgttttga ttttaatatg tttgctgctg ctaccaagag ctttgtcaag caagttggag 60
atggagggag attagttcct gttccaagcc tcagtgaagc tgacaaatat caacctctaa 120
<210> 95
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 95
ggccacattt ctttatatta aaattctagt ttgtacattt cttttagaaa caattacatg 60
ttactttgga atcatttctt ccatgcttcc tccataaaga ctgataagtc ttggatgcaa 120
<210> 96
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 96
taaaaacaaa aatgggacaa atactgggtc attattcctc caaaggtgag ctggtattat 60
gaagaggctt tgatttcttt ctctttatca ggatattctt ccacctctga ggagaacatt 120
<210> 97
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 97
gtccattaat tatctgcggc aaaactggga tcctagtaga agcttcagca tatggatggt 60
tcctctactg cctgggcacc ctctgtgaca gctttgacac atttggccat tgtctgtgag 120
<210> 98
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 98
aaagaggaca tatttcagga gaaatctatg actctcctca gagagcccac tctggcattc 60
gtggcccccc agtcctggta gcgcctatag tcccctggca tcagcaggta ctgccgtcct 120
<210> 99
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 99
agtgttaagc tattttaata caaatccacc actctccgca aagagcctgc cttagcatcc 60
atggcccccc agtcctggca ccgcctgtac tcttggggcc tcagcaggta ttgccgcccc 120
<210> 100
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 100
aaggcaggca cagcttagta caaatcggtg acccgtctca aagagccgac tttggcatct 60
gcaccccccc agtcgtggta ccttctgtag tccccaggcc tcagcagata ctgccgcccc 120
<210> 101
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 101
tttgcttgtt tcttttgttt ttcagactca tgaaatggcc acagatgata agacgtcccc 60
aacactggac tctgctaatg atttgcctcg atctcctact agtccttctc atctcacaca 120
<210> 102
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 102
tttggtgttt ccctttcaag atgccacttt cagactttat tctggctctg aaggacaatc 60
cctactttgg ggctggattt gggctggtgg gtgtgggcac agccctggcc ctggcccgga 120
<210> 103
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 103
agggaaagga gacacatctt attcccttct gggttagtct ttgagaggga agtggccaaa 60
cttttgtcac cgttccatgg tctgaggctt agtgtcttca gaggtttctt cctgtccctg 120
<210> 104
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 104
agcctgaggg tccccggact cgcccaggct ctgagcgcgc gcccaccatg agcgcccgga 60
ccgcccccag gccgcaggtg ctcctgctgc cgctcctgct ggtgctcctg gcggcggcgc 120
<210> 105
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 105
tcctccctcc aaaaaaccca aattcttgaa atgtacacac attcttcttt caaacataga 60
aaagtctcac tctggatgtg cttacaagaa gatcgtcgtc aaaaaacttt gtttctatga 120
<210> 106
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 106
aggagagagg acaggggtct ctcaccccag ctcctggtca ccatgctgct ggctgtgctg 60
ctgctgctac ccctcccaag ctcatggttt gcccacgggc acccactgta cacacgcctg 120
<210> 107
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 107
attaaaaaat ggataagtct tattctgtca gtcctgtttc agagatctga aaattgtcaa 60
tgctttgtcc attgatgtgg atatccaggt cagtcctgtt tgggctttta gaaaccagag 120
<210> 108
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 108
tctaacacct gaccaacacc ccaaggtggt agaagttgcc agggacagat aacatggcag 60
ccagcgggaa gaccagcaag tccgaaccga accatgttat cttcaagaag atctcccggg 120
<210> 109
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 109
tgccaggcct ctgccccaac tcacttacct attcccaggc acttaggagt cttctccaca 60
cacagaccat tctgagaaca gggagcccag tggagaactg agtaggctga cccatctgtg 120
<210> 110
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 110
cgcgcggcgg ccgctgcggc gcgatggcgg cggggctggc gcggctcctg ttgctcctcg 60
ggctctcggc cggcggtgag tggcccggcc gtccgcgtcc ccaggccccg cgcgcgctct 120
<210> 111
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 111
cctggggccc aggcccctca ggggtcgtca tctgcctctt ccaccacctc ctcgtccagc 60
tccctggcgt cctccatgct gttgtggcgg acctgctcgg ggacggtgcg tggcagtggg 120
<210> 112
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 112
ggcaggcctt gcgcgggcaa catggcggcg cccggcgagc ggggccgctt ccacggcggg 60
aacctcttct tcctgccggg gggcgcgcgc tccgagatga tggacgacct ggcgaccgac 120
<210> 113
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 113
cggagctccg agcatccctt agttttaagt catggcgggt gcgaacgggt ctctgctgca 60
ggcggctccg tgacagctcc tgcttcacat gggtagagga gagacggcaa acgtcggggc 120
<210> 114
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 114
acttccatct ccagcactgc aggctgtgcg ggctggtcca actggggtac ggttgagggt 60
cctggctcag accaggcctg actcctgggc cagtctgtaa aacaggccct tctgggccag 120
<210> 115
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 115
ccactcctcg tgcccagtgc tacctgagcc ggccatcagg tttgactgcc cccagctccg 60
acttggggtc agggctgaag gagctccagg ccgtctggct gcaggtctgc atgaccgggc 120
<210> 116
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 116
tgtctgacct gtccctctgc ccacaggtga atgtgacctg cgtcctgagg atcccgagta 60
tcaggagagc tggcagagga agtcatgcag aggcaaagcc aggagccccg acagggagat 120
<210> 117
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 117
tgtaatgttc aaatggccct ccccacggcg tctccggcaa gccttctgcg gagaggtgtc 60
ctgttgagcg atccctcact gtgcgtgctg gctcatcgtg tggttctgca aaggcgaaga 120
<210> 118
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 118
tcatgtagga actttcatgt gagcatttac tgaggagtag gagatgcctc tggagcattt 60
gctttcaagc ctccaagtca tctctgtatt gcttgtagac agactggagg gcatggagag 120
<210> 119
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 119
agcaaaccag cataggtttt taacagaaaa tctttgccag gaacttcatg acctgtattt 60
tcctcaccta ggaagaagct gtccccactc gcatgatttt gaacagtgtg ttgatgttat 120
<210> 120
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 120
ccgccgggcg gccgcgccct gcatgcgagt tgggccgcgg gcggggttgg agcctactcg 60
gggcgactgc gatggacgcc ttagaaggag agagctttgc gctgtctttg tgagtagctc 120
<210> 121
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 121
tggaactgcg cgcgggatcc gcctcacccc accttactcc gccttctcct ccctttcctc 60
cggcatcttc accgacagga tctgctctcc cggctccggg cccgggctca tgcagatcct 120
<210> 122
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 122
cggctttgac gagcccgcag cggccgggcc cgagcgcaga gccgggccga gactgcacca 60
tgcaggaagc gccagctgcg ctgcccacgg agccaggccc cagccccgtg cctgccttcc 120
<210> 123
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 123
caaaggaagg tctttattca ggaggcgggg gctctgggct ggcagtcggg gatgcagggg 60
gaccctggcg gtaggcaccc agcaggatgg cattgatgtg ctccagggtc aggttgctga 120
<210> 124
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 124
aggggcaaga gctgagcgga acaccggccc gccgtcgcgg cagctgcttc acccctctct 60
ctgcagccat ggggctccct cgtggacctc tcgcgtctct cctccttctc caggtactcc 120
<210> 125
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 125
agtccagcct tccctagatg ggacacatcc tcaggagaat ggatgcaggc ctatttatca 60
cactcctcct ggctgccttc agcacaatgg tagtgctgct cacagaactc ctggatccgg 120
<210> 126
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 126
agaaatccca gatcctgtga tgggggacac cagtgaggat gcctcgatcc atcgattgga 60
aggcactgat ctggactgtc aggttggtgg tcttatttgc aagtccaaaa gtgcggccag 120
<210> 127
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 127
agtcagggaa cttgtgggct aagtgctcag acccccttca cagcactttg cctggattca 60
tgaggccttg ggggtctagc acggccttga gctgccgcat ggtctccacg cccacggcgc 120
<210> 128
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 128
tcccgggcct agcgcctgct acaactgtgg cctccactaa tttcgggggt gcgaggcggt 60
ggatgatgcc caagtgaagc cgttcaggcc tcgtggcagc accagatcaa gggcgaggtt 120
<210> 129
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 129
ggtcctgctg agtcttcaga aatggccttt ctaggataag tgctccttac acaagtctcc 60
ccggggcgtc actgtcacag gaatggtggt caccacctga ttctgaaaca cgaagatctt 120
<210> 130
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 130
tgaatactcg gctgggctgg ggaatctgct tctggaaggc tttctcacat agcccagtgg 60
ttgtcaggaa acctcagtcc ttcttcatgg actgcttgag gaccatcaca acatggtaac 120
<210> 131
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 131
aaaaaaaaaa actcaagcac atgcaaatca aaaatcaaga tgtacctctt tgacttcagg 60
caactggatt ttttcacatc actgatgtag aggattccct tgggtacctg atttagtaaa 120
<210> 132
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 132
tgtcactcag cctggacgcg cttcttcggg tcgcgggtgc actccggccc ggctcccgcc 60
tcggccccga tggacgccgc gttcctcctc gtcctcgggc tgttggccca ggtaaggcat 120
<210> 133
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 133
ctccatgtta tgcttgtaat aaattattta cacgggagct gggctggtgt gcagtggcag 60
gtcccgtcag aagagatgag gctcctggga caggtcagcg tcaggggcag cctgctgtct 120
<210> 134
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 134
ctccaacgac tccttcctgc ttcctggaca ggactatggc tgtgcaggga tcccagagaa 60
gacttctggg ctccctcaac tccaccccca cagccatccc ccagctgggg ctggctgcca 120
<210> 135
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 135
gctgaaattc cacacgacca gcgtctgcag cagcagcacc gtgagcgccg cgagcagcgc 60
cgagtgcgag cgccgggcca gcctccgggc gcacggcgcc gccaccatct tcggagcgcg 120
<210> 136
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 136
gagttggttc atgttcttta ttgaacacct tacatgggta tggacagggc ctatgggtgg 60
ggcaaggcag caatgacagc ctcagtgaag tcatggcaag tagcatagcc acccatgtca 120
<210> 137
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 137
ccagcctggg aggacgtgga gggctggcga atggggcgtg ggcagggtct gccagtcaag 60
gcctgtgctc agcgtccatg acatccaagt acttggcttc aatgattcgg ggcagcagga 120
<210> 138
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 138
cggaaggagg gggtggatga ggaggctcgg gcccttccac ccacgcgtcc attgggcggc 60
gccgccatca ctctcttctg ggctacaccg ccttctcttc ctccgcggaa cccggatccc 120
<210> 139
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 139
aatacgaaca tgctattctc ttagttttta tcttcaataa catatgaaag atccaaaatc 60
aaattggctg tctctacagc cacctgtagg ccactaagct ttgcagtcaa acagtccaag 120
<210> 140
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 140
cccgcggtct gctatacgat gtactccatt cggtttaagc tctgggcact ttccaagtct 60
ctgttgtcct gtttgtttgt ccagtttggg tgttttgtcg gcgtgccgtt ggggggcttc 120
<210> 141
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 141
gcagctggag atgctgcggc cggcagggct ctggcgctta tgtcggcgac cttgggcggc 60
gagggtccca gcggagaatc ttggccgtag ggaagtcacc tctggtgtct ctccccgcgg 120
<210> 142
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 142
aaatacattt tatcccatca agcctgggca ttttaagagc tcttctctcc tgatttcttc 60
ttgtctgact ttgtctttcc aatcatggtc tccacgatca cagcggtttc ttcatatgtc 120
<210> 143
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 143
ctgcaggcgg cccggcgcgg tgtttacccg cggtgcatgg tggggcggtc tccttcggca 60
accccggccg aacggccacc cagaggctgt gctgagctgg cgcagcggca gcagcatgag 120
<210> 144
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 144
gcacttctcc agaggggcag actttagcag cattacaggg ttcagctgaa ggtcatcagg 60
caccaccacg gggtctgggg agcctgtgtg tctcctgcaa agtccagtga acgggagcct 120
<210> 145
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 145
tttgggttag ttttagtttt gtctgctgct cttcgatgac cgtgtcacct gttggcttca 60
gtcacaccag ctctgaccac atgctgggat ctgggctgga acctgggccc tgctgaggtc 120
<210> 146
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 146
tgaatcctag aaaataatct gtctttacac agagagggct gcgggggcaa tcctactgac 60
gagtttgttg agagcgcgaa ctttttcttc caagaggcgg ctttgtttct cggcgcctcg 120
<210> 147
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 147
ggaaaccctg gtccctccaa gccctgtcag aaatccaggg gggtgaggtc cccaaagtca 60
cagtcgaaga ggtctctgat gccctcgccc tcctcgaggc cgaagtggta gtcgagggcc 120
<210> 148
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 148
aaggccggcg cggcgagcag caaccatgtc ggtgttcggg aagctgttcg gggctggagg 60
gggtaaggcc ggcaagggcg gcccgacccc ccaggaggcc atccagcggc tgcgggacac 120
<210> 149
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 149
gggggcccgc gcggtcacgg cttctcgtga cagtgtttgc aaagcgcgga gggcgtcccg 60
gagaaggcgg cgcacttgtc agggcagggc cccagggcgg cgcctgcgga gaaggggcac 120
<210> 150
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 150
tccctcttcc gctgcctcat tcctttcctt cctagccttg gtcgtcgccg ccaccatgaa 60
caagaagaag aaaccgttcc tagggatgcc cgcgcccctc ggctacgtgc cggggctggg 120
<210> 151
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 151
ctgcgcggcg gagatgatga cgatctgcga ggagtcgagc agccgcagcg cgccggcccc 60
gagcagggcc tccagcgccg gcgcgcatgg gccgcccgca ggggccccgg ccaaggccat 120
<210> 152
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 152
attttcaaca ggtgactttt ccacaggaac ttctgcaatg tcccatcaac ctctcagctg 60
cctcactgaa aaggaggaca gccccagtga aagcacagga aatggacccc cccacctggc 120
<210> 153
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 153
ttttccttag acatttcgtt tggttttggt tttctgttga aagagttata gcagatggct 60
atttcaaaga ttggttcttt ctcctttcac aagtcgagct cgaaacatcc aaatatcctg 120
<210> 154
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 154
atcgccaccc tccgcagcca tgtccaccag gtccgtgtcc tcgtcctcct accgcaggat 60
gttcggcggc ccgggcaccg cgagccggcc gagctccagc cggagctacg tgactacgtc 120
<210> 155
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 155
aagattttct tgtgaacacc acaatccagt tcattctgag gtcatccagt tccagtagtc 60
ttcttgagga aaacaccatt ttcctcagtt cagttttctt taaaaagaaa ataaaaacca 120
<210> 156
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 156
taaagttggg acccgcgggc cgggcgcgct cgcgtaacgg ggattagagg cgcgggggcg 60
cgggtcccta agcgcccctc ctccctggcg cctcccgctg cccggcccag agccccggcc 120
<210> 157
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 157
ctgccctggg gctctgccta tggggccctt ccctacaggt ggtcctgcag gcctgggctc 60
cgcttcaccc tcagctggtt gtgctggagc atctccttgg ccctggcgag agcctcttct 120
<210> 158
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 158
gggacttgaa agtttaggaa gcaatgttgt ttagcagtat tctggcttga gtttccaaat 60
tccttcacca ccagaagttc tatggaaagt gcacagattt ctcaatagtt ctcggaagac 120
<210> 159
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 159
aatatctttt aaaaaattgg tcacagtttt gtcattggta tatggaggtt gttccagggg 60
cccatccaaa gctcttcatc atcagactgt gtgtggtcac tatgaaattc caaagcctcc 120
<210> 160
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 160
aggcgcgcgc cctaggtggc catctggaag ggctcgggct ggaagccgaa gagtctctgg 60
ctgtacagct cgctctcgcc cggcagcttc gcgcccggga acgggaggta gtggtcgcgg 120
<210> 161
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 161
accgacaatc ctctgaccct gccttctttg aacttggcct ttctctaggc gttcaagacc 60
cagctgttga gagtagaaaa gcagaagaaa ggacccgagg tcagcaagtg ccctccccac 120
<210> 162
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 162
gcggcgagcg gcgagcggcc cgcggagacc caggagctgc cggcacgccg cggatgagcc 60
ttcgcgccgg cgggaagacg cggcggtggc cagggccaga gcaggcggcc cgcgggggcc 120
<210> 163
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 163
ctccgtcccg cgcgcccgca gcgtcctggc cgccatggcc gggctcgtgg tgcgtggaac 60
tcaagtaagt cccggccagc cccgcctccg cccggggcgc agtccgcgtg gggagggaca 120
<210> 164
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 164
catgctccgt gcccctgcgc ccggtctcgg cggcggcagg cgacactccg cgccggcgga 60
gacatgaggc gctgccggtg ggccgccctg gccctggggc tgctgcgcct ctgcttgggt 120
<210> 165
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 165
gctgtgggaa gccagagaca gctgggccac tggcagtgag ggagagtgag gatggcagag 60
accagtgccc tgcccactgg cttcggggag ctcgaggtgc tggctgtggg gatggtgcta 120
<210> 166
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 166
ctcacagcgc ccaggtccgc ggccgggcct tgattttttg gcggggaccg tcatggcgtc 60
gcagccaaat tcgtctgcga agaagaaaga ggagaagggg aagaacatcc aggtggtggt 120
<210> 167
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 167
cctaagataa atagagctga agactgagag ctaatcagaa gttctcagat gaaactagat 60
tcttgatatt gctacaaaag gtttctttct gatctgccat cgcagtaacc tggaaaatac 120
<210> 168
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 168
ctgaacaaac gcagcaaagc aagccacacc atgggtgaga tcaaccaagt tgccgtggag 60
aaatacctgg aggagaaccc tcagtttgcc aaggagtact ttgacaggaa gttgcgggtg 120
<210> 169
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 169
agggaaaact gaaatctgat tattttcttt tcttcacata gtctctaggt tgagaactcc 60
aagtagtaag aggcacagga taaggatggg agagactgag catttttgag agaatactcc 120
<210> 170
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 170
ggaggacagg atgcaaaggc agactgcggc caccttggcc tagagcaggg ggatacggga 60
ggcctcccag aggcgccggg ccagctggcc ggcctgctgc accagcaggt cagtggggat 120
<210> 171
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 171
gtcattctgg gactgttttc agttgccact gtcatctatt atattagcaa tattgggttg 60
ctccatcctc aaatcagatg tttctgatgc cttcatccaa atcactgatt ttaaaagtaa 120
<210> 172
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 172
cgagacgcgt tgtcgggccg cggaggagcg ggacagccag acccagcccc aggacgcggc 60
ggccgcgggg agcctagcat ggaagagcgc gcgggtccgg caggtaagag cggcagagac 120
<210> 173
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 173
ctgaggttag ggggagggtc atgggatgcg tggggagggt gcgggcttag aatcaggagt 60
ctggagggct ggggaggggg ctgggctggg agttggctgg aggagcctgg catcagtggg 120
<210> 174
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 174
cgcggaggct gtgaatcgtt gcccccgccc tcggggatga tctacgggcg gggccgctca 60
tacgggcctt tccacggcgt actggcacgg actaaggttg gctgccgggg gcggcccgtg 120
<210> 175
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 175
ataaggcagg ttgttcccta aagtgctcag ttatcattca gtgggttatt tgccgttgat 60
ccttttctgc catttcttgt tggcgaattg tattctgtgc tgcttgatgc attttctcta 120
<210> 176
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 176
aattgctgct ggtgggtgaa aaaaaaatgc cgcagctgaa cggcggtgga ggggatgacc 60
taggcgccaa cgacgaactg atttccttca aagacgaggg cgaacaggag gagaagagct 120
<210> 177
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 177
ttacaaaatt aacaaggaag gcagaacgca cgtccacctt gagtcctact ggtccacagc 60
cagtacgcac ggcaggtgag aggggttaca tggtggcttg tggcagtcca ctgctccaga 120
<210> 178
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 178
cagctggctc ccagggacca ctgaaaacag ctggctaccc tcagaaagac aagatggtct 60
tgttaataat ttcaatggac tctcgaagct catcctcctt gatcaccagc ggaggcgcaa 120
<210> 179
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 179
gtcattctgg gactgttttc agttgccact gtcatctatt atattagcaa tattgggttg 60
ctccatcctc aaatcagatg tttctgatgc cttcatccaa atcactgatt ttaaaagtaa 120
<210> 180
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 180
tagcttgatc ttgtctagaa atctaagtca tcttggccaa agccgtctgc cctgcccact 60
gcctcagtgg gggcgagact tccgagtgcc tggtcctctt gtaggtcata accttcactg 120
<210> 181
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 181
ggtggagtct ggggagggcg tgtgggtggc agtcagcggc gtttgagtcc gccattggca 60
agctgggctg ggtgtcgggg caggcagggc tcctcgggca gcggctcatg agagaagacg 120
<210> 182
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 182
gaggcgaccg agtgctgaga ggaacctgcg gaatcggccg agatggggtc tggcgcgcgc 60
tttccctcgg ggacccttcg tgtccggtgg ttgctgttgc ttggcctggt gggcccagtc 120
<210> 183
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 183
ttcttctcta ggtctcagcc aaaatgagtg ataccccttc tactggtttt tccatcattc 60
atcctacgtc ttctgaaggt caagttccac cccctcgcca tttgagcctc actcatcctg 120
<210> 184
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 184
ttagttagga agccattcac atgacataac ttctcattga atgctctcag aaatgctgct 60
gagataatct tcagacttca ttataaaatt tgtccattcc tgacaaatgt cctccatgga 120
<210> 185
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 185
ggctgggcct gaggggcgga gccccggccc ggaggtctgt catgctgttc ccgctccagg 60
tggccgctgt aacctcttcg gtccgcgacg atcctctaga gcactgtgtg tctccccgga 120
<210> 186
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 186
tctgtccctg gtcacagagc agctgtgcgc tgtcaaagtg ccgagcgctg ttacaggtac 60
tccagttcca aggcatggtg cagggccatg aggtccgagt ggctggagat cctccagaag 120
<210> 187
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 187
caaaggaagc aattttagtt tctaaagagc atttacttct gacatctcga ttcctgattt 60
cagtggctgc agactgtgta cttctgtgcc acacttagga caagtaaagt acacgtcaaa 120
<210> 188
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 188
ctaaagataa tcaaacattt attgcttagc cttttctttg acttcaatag taagaacctc 60
ttctccgccc tcagcagaag gagccatgct gataatgagt gattgacgag aagtccctct 120
<210> 189
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 189
tggggggctg gaggctgtga ggcttccagc gtcggtacca tggcgacgcg cggtggggct 60
ggggtggcaa tggcggtttg gtccctctta tccgcccggg ccgtgaccgc gttccttctg 120
<210> 190
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 190
ctctcaacaa cccatgaagt gtctggagct ccaccctctg ctcaagagga acggcacatg 60
acccaggcct atgccaatca gcatttcaca ttcctcatgg ttggttcaaa ggtgagcaag 120
<210> 191
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 191
ccactctcat ggatgcatct gtccgaatgt gcagtctgca ttcttaatgt ttaccacaaa 60
cccagcgaag ctgatgatct gtctgaaggt cagtcacttc ttcaatagtt ctccagctac 120
<210> 192
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 192
tccgggccaa ggcggcggga aaggcaccgc tacctgcagc cgcacgactc caccaccatg 60
tcctcgtact gcttgtagac cacattattg cccgcgtcga tgtatagaat gctgatggga 120
<210> 193
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 193
tctgtctact cctttcagct tccgacttcg actccttacc ttaaaagatg ctggagtcat 60
atgtaactcc aattttaatg agctatgtga atcgctacat caagaactta aagccgtcgg 120
<210> 194
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 194
tcactgccct agcctctgac aaccccagct ctacccgaca tcccccaatg cagtgcagtc 60
agcgggccac ctgcaggagc tcttccgtgg ccaggcccac cagggcatgg aagctcaggt 120
<210> 195
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 195
gagccgcttt gggcggcggc cccgggaacc cggagcgcta aggagaacgg acctcagagg 60
ttgtctgaag gccgaggcca agatggcggt gctgtcaggt gagcgcggca ccggcggcgg 120
<210> 196
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 196
gggcggcagg ctcaggctgg cggccaggcc ctgtccagag cctgtgcatg ttccttggcc 60
agcagccgca gggacgcctc ctccagggcg aagccatctg cgaaggtggg agcaaaggca 120
<210> 197
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 197
cgcagtgaca gcggcgggcg atcggaccca ggctgccccg ccgtacccgc ctgcgtcccg 60
cgctcccgcc ccagcatgac agccccggcg ggtccgcgcg gctcaggtga gggcgcgggc 120
<210> 198
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 198
caaaacacca gtgagcttct gtcttgctgg agggtcggct ttgggcggaa ctggctttgt 60
tgaccgggag aaacgagatg ggggtgaagc tggagatatt tcgggtcagt ggacacagga 120
<210> 199
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 199
gccggcccgc tgcagggctg gcggcggaga ccccgccagc tgtggctccg ggtgccgcgc 60
gccccctagt ggccatggca ggttttgcag cacatctggc ggaagtaggc tcggctgcag 120
<210> 200
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 200
gagtcagagt ctggtctgcc cccggagcag gaggcttggg cttggagggc ccatcccagc 60
agacctaacc atccacctcc ttccattgaa ctgaggccag gaaagtgcgg atttccatgg 120
<210> 201
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 201
ggcgcgggcg gccgggccca gccggagccc accgcgatgg cgagggagga gtgcaaggcg 60
ctgctggacg ggctcaacaa gacgactgcg tgctaccacc acctggtgct gaccgtcggt 120
<210> 202
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 202
cgcgccagaa tcggatccgg gaagctgcgc gccatgagca gctccctgcg gccgggcccc 60
agccgctggc ggcgggcggc cagcatcgtc ctggcggctg gctggtcgcg cccggagacc 120
<210> 203
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 203
catagagaac tttaatgagt atgaattctc tggtgtctaa tgtgatgtga cttttggctg 60
aaagctttcc cacattcact acactgataa ggtttttcac cagtatggat tctcatgtgc 120
<210> 204
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 204
cagacagcag tcacctccaa gaccagggcc ccgagacctc ctatttctcg gacgccggcg 60
caactggggg tgcaggcggc gggtctcgga ggcagaggtg ggctcgcacc tcctgcagct 120
<210> 205
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 205
tgcaggcccc ctgacttggg cctcagtgtc cccgaagatc atgatggcgt atatgaaccc 60
ggggccccac tattctgtca acgccttggc cctaagtggc cccagtgtgg atctgatgca 120
<210> 206
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 206
agctttgttt taggggtaac tcccctcgcc ttgtggggag gcttaggacg ggcgggtgca 60
atcctcgaag gggagtctca gcgaccatgg gggacaccat ccactgtaag acagagaaca 120
<210> 207
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 207
tcggccatct cctgcttctg ggacctgcca gcaccgtttt tgtggttagc tccttcttgc 60
caaccaacca tgagctccca gattcgtcag aattattcca ccgacgtgga ggcagccgtc 120
<210> 208
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 208
gcacatttga ttttactggg ggtaggggac agggctgggc gttcaggagt tctccgctat 60
ggtgtcctcg atccacttca cataagacag cactctgacg gcgacagaag gcttattggg 120
<210> 209
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 209
tcctcctctt cagtggcctc actttaactt ccagatgaag ttgggggaca catttgggct 60
cagcggggtg tagacaggcg ccggcattca tgcacccggt aggcgcacat gtagaaaatc 120
<210> 210
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 210
ctgcatgcta gtggggttga taaagaagga ccagggtctt ctgggggaga atcatcgctc 60
agtaataagg agggactttg tcggggcaag tttttaggga acgctgctgt ccctccccag 120
<210> 211
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 211
gtgatggatg aagtttcctt tagtcttcga gcaatagatg gatgtctatt aattccatcc 60
caccaccaca tgagccacaa ggagataaca catcccaaca gtgcaggaca caaccatcat 120
<210> 212
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 212
gactgttaaa aaaaaaaatt aaagaaacaa aacagactag cattcagttt ctgtggaagc 60
tttctctttc ttaacctttt tttcttctgc tgtcattcca gacacaatta ctaagctgct 120
<210> 213
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 213
agtttaatcc tcctcttctc ccttcctgaa gctgcacgct gcagtaagag cacagcagaa 60
atgcagacaa aagggggcca aacatgggcg agaagggctc tgttgctcgg catcctgtgg 120
<210> 214
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 214
tctctggatg gtgaattaat gaagcaaaac ctggattttc atcttcagat tatcacccag 60
ttcaccactg aggtagtctt tgtcatattt gtcttctagt tggttaagtt ctttcttttt 120
<210> 215
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 215
atataactgg ccagcatggg gaaattgatc aggatggggc cgcaagagag gtggttactc 60
cggacaaagc ggcttcattg gagtcgcctc ctcttcttac tgggaatgtt gatcatcggt 120
<210> 216
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 216
ggctgggcca ggctcagcaa cccaggcctc ccgcaggcag gcagaggctg ccctgtaacc 60
catggagacc agaccaacag ctctgatgag ctccacagtg gctgcagctg cgcctgcagc 120
<210> 217
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 217
ggctgagcta aaatggctga ggagagagtc gcgacggtga gcgccgagat tctctttagt 60
tgcccggccg cagggcaagg caagctggcg ggtggctttt gtcccatgca gcggttcctg 120
<210> 218
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 218
tcagcacaga tgggcaggtg ggttgacctt ccctgccctc agggctgggc accattggca 60
cccaacaggg ccgtcttgcg gaagacctgc agggttgggt tgtgcagcag cgtgtaggcc 120
<210> 219
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 219
tttcctgggg agcttggagg ccacacccat gtgactgcag ggcaggatgg tggacttcta 60
ggcccctaca gcctcggcag tgtcctcaga tccggatgtg gaaagtgcta gaaagagaga 120
<210> 220
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 220
gcctcccagg gactcaggtc actaatggag gtggcttggc ttgtctatgt gctgggccaa 60
cagccactgg cgaggcaagg cgagggtcag tcacggctgg tgccaggaag agggctggtt 120
<210> 221
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 221
atcatccaac tccttcctct tgcattctta aatatggctg cctgtgttct cattgcagag 60
ctcctctgac aaggagtcca tcctgaccat cctcaaggtc ctcggagatc tgctttctgt 120
<210> 222
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 222
cccagagatt ctaactacag ttcagctggc aggagatgtt ttcagaaggc tgtgttctca 60
gcttgggcct gggggccaaa gagctgctca gcaacggcct tgccatcata cttgggcagc 120
<210> 223
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 223
tccaaggcag ggtctgcccc gagggcttac agggggtttt tccggacatc aatcatcccc 60
gggctggctc gtcgcagttc ccgccggggc ttggacaaag gcccaaagtt ccagggcagg 120
<210> 224
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 224
tcagggggcc aacaaagcat ttatttcacc cgccaaacta aaggagagtt attccagttt 60
aggaggaaga tgcaagcggt ttgggacctt gaacaaggca aatatggttt tggtatgttt 120
<210> 225
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 225
gacctgagtc tgagctttga gcgacccttg ggactgcgtg tcacgagttc ggcggtggct 60
ggcgcatctt gtttgcatag aagtccctgc aggtctggca gcagcgctgg taccaccgca 120
<210> 226
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 226
gggtatcaaa cggaggagct gcaggtgagc cagtcggcat ctttgcaact ggctcctgag 60
accagacgcc tcgggtcaca aggatgtctg agtgtgaggt gaggtggctt tcattcctga 120
<210> 227
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 227
cctggagcag cagcgtctcc agggacaggc agccaccatg agcctcagtg aggagcaggc 60
ccggagcttt ctggaccaga accccgattt tgcccgccag tactttggga agaaactgag 120
<210> 228
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 228
cctctgcagg ctggccaggt tgaggggctg ccttttttcc ccaggtgctg atccaagcct 60
ccctctggaa taagctgtat ccaccggctt ctcgtgaggc tggtgtgagg gtcccgcatt 120
<210> 229
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 229
cgcggccccc gcccccgcca tgggcgcccc tgcctgcgcc ctcgcgctct gcgtggccgt 60
ggccatcgtg gccggcgcct cctcggagtc cttggggacg gagcagcgcg tcgtggggcg 120
<210> 230
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 230
gatgtgcctg accttgactt ttcttccagg caggatggac tccaacactg ctccgctggg 60
cccctcctgc ccacagcccc cgccagcacc gcagccccag gcgcgttccc gactcaatgc 120
<210> 231
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 231
gtgccccctg tgggaacact gctccacgcg gtcactcggg ggtccaggct gacaaaggct 60
ccaccgtgtt gtggctgcac catccagtcc ctgcggcctc ctccgccatc cagaggctcc 120
<210> 232
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 232
aggtgaaggg cagccagaac tatcaacaca aaagaaaaat gcgctcactg aactgcacac 60
atagagcttc taggagggtt aacagtcctt gacataggtt cctggttgta gtttacaata 120
<210> 233
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 233
agcaatattt cattgatttc aaaatgcctg acttcttcat tgttctttgt cagggaccca 60
tcccagccaa aatgaatccc agggaagaaa aagtaaaaat aattacagag gtaagtggcc 120
<210> 234
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 234
tctaaaaact aaaaactata gtcctgtaga ttctcttcaa tgaaagcatc aaacttacct 60
caagcagttt caacatcagc tatcaatgtt gtgatgggct aaaaaacaaa aaaataaaga 120
<210> 235
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 235
acagtttata tgaatttctg cctaggaagc ccagcaatgt tacacagtag tttcgtgttt 60
ccataatcca gtcctaacat tgccagtgct atcggtaccg agcaagggtc cctcctgccc 120
<210> 236
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 236
ttcaccagct gctggagcac acctcaggca ccctgcgttc cacctgccag cagctgcatg 60
tgctgcttga caaagagaat caatgtgtct cgagaaaagg caagtattct tttttttagt 120
<210> 237
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 237
ctgggtgacg ggaacgcggt agcctgcttg gtggagaccg ggtgcgcctg cgtacttcat 60
agttcgcgta gcggctcgag cgtggagatg aaggtaaata acttacaaat tcccggggtg 120
<210> 238
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 238
gaccagcttt tcggttctat gtagagtcga tgggcccact ttccgctcca ggtccctcaa 60
tacttgaaaa ttctgtgtca ataagcggtt tcagaaattt ctcaaccttg gttaatcttt 120
<210> 239
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 239
agaaacttat taggcaagaa caggtaatgt agttatccat gactactttt aaccatgcag 60
actaataata ttctggaggt ttatagctcg gcaccttcac ctgtttaaaa aaaaaaaaag 120
<210> 240
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 240
tgctcccctc tcccccggaa gcccttgttg ctagtagggg gctgcatggc acgcgcccct 60
ttgcgtccag agaataccat agtgcggggg agcgccaacc agtgccagga cacttaccga 120
<210> 241
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 241
caggttctga atgcttagga ttcggttttt accttttgtt taaataacct ctaaaaggag 60
caatgcatct ctttgcatgt ctgtgcattg tccttagctt tttggaagga gtgggctgtt 120
<210> 242
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 242
cggcgctgtg ggtgcggctc acacgggccg gtccactgca tactggcaag cactcaggtt 60
ggaggccggg ttctgcacgc tggcgtagcc gaagctggag tgctgctttg ctttcagtct 120
<210> 243
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 243
aacatttttc atttaaacag accacttctt tgggacttta ccttatttgt atgtcatgct 60
gcatcgaaga atgaaatcaa tgcattttgg tcataactgt ccagaatttc caatagaagg 120
<210> 244
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 244
gcagatcatg atacatggtg atggcttgca gagtcgtaaa caaaagaaga cacatgggac 60
ttcaacaact ttcatcattc gcggaaacag gaagaacttt cctaggccca ctaaaatcat 120
<210> 245
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 245
acctctccaa gacgccctct tccctgcagg atgaagaacc ccatgctgga ggtggtgtct 60
ttactactgg agaagctgct cctcatctcc aacttcacgc tctttagttc gggcgccgcg 120
<210> 246
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 246
agcaggactg atgtgcacac agaaggaatg aagtatggat gtgaaagaac gcaggcctta 60
ctgctccctg accaagagca gacgagagaa ggaacggcgc tacacaaatt cctccgcaga 120
<210> 247
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 247
gccagccggg gcgatggcgg ggctctggct ggggctcgtg tggcagaagc tgctgctgtg 60
gggcgcggcg agtgcccttt ccctggccgg cgccagtctg gtcctgagcc tgctgcagag 120
<210> 248
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 248
cctcccccgc cagctcgacc ccggtgtggt gcgcaggcgc agtctgcgca gggactggcg 60
ggactgcgcg gcggcaacag cagacatgtc gggggtccgg ggcctgtcgc ggctgctgag 120
<210> 249
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 249
actgtctctg aggttagggt cattgtctct ttattgacct aatccacata tctaacaaag 60
agagcgacaa agcaacagcc tatcagtgcc accgcagacg ctgtgatcac cacgacgaca 120
<210> 250
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 250
cctgtgcatg gaaacccaat gatatccagg tcttacaggt ccagggccca gtggacagac 60
aggccctggt cctccacgct ggccaccatg tcttcgatgg cattccagtc cagcttgctg 120
<210> 251
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 251
cggtgatccg tcctttcatc ctggcgtttg cctgcagcaa gatggcggcg gtgtcaatgt 60
cagtggtact gaggcagacg ttgtggcgga gaagggcagt ggctgtagct gccctttccg 120
<210> 252
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 252
cgctgctggc agcgctggaa actgggtgga cggcatgggt tggtctcagg atttgttccg 60
cgccttgtgg agatcgctgt caagggaagt gaaggagcac gtgggcacgg accaattcgg 120
<210> 253
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 253
ctctttgttc cgcagccatt tcaggccccg gacaggaggc agtgccgctt cggccgaagg 60
cccgagcgcc cgaggcgtct gggatggtgt gggaccgggt aggtggcgtg agggtgcgcg 120
<210> 254
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 254
tgaatgcttg tgatttactt tgtgtttttc ttcatttcta ggccaagatg ttcataaata 60
taaagagcat cttatggatg tgttcaacct taatagtaac ccatgcgcta cataaaggtg 120
<210> 255
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 255
cgggcaagga gtacggacgg gagtcagagg cagagcgagg gtgtgtggag ggccggcggg 60
gaccgccggg agcgcgcgga tgtcggtgtt cctggggcca ggtaggctat ggctgagggg 120
<210> 256
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 256
cagcaggcgg gctcgggcgc gccgcacacg ccgcagaccc cgggccagcc cggagcgccc 60
gccacccccg gcacggcggg ggacaagggc cagggcccgc ccggttcggg ccagagccag 120
<210> 257
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 257
tggagggaga actttatgtg ttgcactgag ggcagtctcc ggaaacgcga ttcgcagcgg 60
gcgccggaag cggtgttgtg tctgcagctc tggcagagga ctgttccact agacacgctg 120
<210> 258
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 258
cctgggccca agtctgtgaa gggttaatag agctgaatct gcagcctcat tctgagagcc 60
tccccaagct cccctaggag gtagtcatcc tcattgctct cttccagttt cttaagctcc 120
<210> 259
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 259
ccgcccgctt gcccgtcggt cgctagctcg ctcggtgcgc gtcgtcccgc tccatggcgc 60
tcttcgtgcg gctgctggct ctcgccctgg ctctggccct gggccccgcc gcgaccctgg 120
<210> 260
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 260
gcggcatccc agtccagtcc tggtgtgggg cctcatctca gctccttcag caagctgttg 60
acagagccca gcagctcctg gaagtagccc tcgtcctcac catcctgcag ctccaggctg 120
<210> 261
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 261
agcccaggct ctggggctgt tgctcttaat cctcagtgga ggcttcaggc tttaccactt 60
agaacgttct ccagggctct ggttacctga tcagcactga agttgttcaa agggacattc 120
<210> 262
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 262
cttaacctca aatagtgcca gtcccacttc ctttcctctg atagtttgtt cagttcagca 60
gatgcagagg ggctggcctg ttctcctttt gatttcctcc acaaggtctt ctcttcggac 120
<210> 263
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 263
atctcttccc aggaaagggt ggtgccgtcc agccagcaca tccccagtgg tgttactgga 60
tgcagcagga cttggatgta gttgcacccc ctgggctggg gtttcccccc ggctgatttc 120
<210> 264
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 264
gtcccgttcc cacctgccct gctacttgtg ggcctctgca tcctcgccct tgggtggggc 60
tgccaccgtg gaggtggcct tgaggctccg ctttcgcttc tgcacattct gctctggatg 120
<210> 265
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 265
gcggcgagcg ggggccatgc aggcgcgcta ctccgtgtcc agccccaact ccctgggagt 60
ggtgccctac ctcggcggcg agcagagcta ctaccgcgcg gcggccgcgg cggccggggg 120
<210> 266
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 266
gcactacgga aacatggatg tgtttcttca aatgcacaac ttggataatc aagtaaatta 60
taaagcaaat ttcagtactc ttgcctcaga accgctgcca tctctgcacc tgtaatgtag 120
<210> 267
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 267
tagagctaga gacatgctta taaaattttg ttttctgatg tcttctctgt aggacaaatg 60
gaaggaaaga tttttggaag cagcttcttg tgtccgaccc gctgtgatca gctgctactc 120
<210> 268
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 268
gtactctacg gagcaatgct gtagggtttc acaccataga ctcctaccgg tggcctccat 60
acttggccac ccagtctgtc ctcccatgca ctgagggaat gggctgtgca cggttcacaa 120
<210> 269
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 269
agggagagcc acgtttcctt tcccccacgc tgcctctact tgcctacatt agaccggcct 60
tcaatgcaaa tcttaacctc ctgaggaatt agagaaggct taggaagagt caaaggtggt 120
<210> 270
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 270
gcggtgcggc gagaggaggg gcggaagtga cgtcgtgtgg ggcgggtccg accgcgcaca 60
atgggccatg gagttcccgt tcgatgtgga cgcgctgttc ccggagcgga tcacggtgct 120
<210> 271
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 271
aggccgaagg atgcaaaagt ggttttctgc tttcgatgat gcaatcattc agcgacagtg 60
gcgggcaaac ccctcccggg gcgggggagg tgtgagcttc acgaaggagg ttgacaccaa 120
<210> 272
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 272
gattccaggt gggcctggtt ccgaatggac aggatcagac agagacggtc ctatcccatg 60
aagcagacag gccccagcag cacccctccc cgcctcggtg gggctcccag gtctgagaag 120
<210> 273
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 273
gaatcctttc ccccacgctg acgggctctg ggggcgctga ggggctgctg gggtcactcg 60
caggccagct tcccgtcgaa actggagagg gcgatggcga aggcctgcag ggcgcacagc 120
<210> 274
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 274
gccccctgaa ggcctgagca atgggcaacg tgatggaggg aaagtcagtg gaggagctga 60
gcagcaccga gtgccaccag tggtacaaga agttcatgac tgagtgcccc tctggccaac 120
<210> 275
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 275
tcctactacc ctggaaacct gggtgagcct ggagtcgtgg aggggcccca gactcctcag 60
aacatggcac ttttccgtct ctgctgagcg agccagctgc tgggattcat gtccatctgc 120
<210> 276
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 276
gggccccagg gccctcagcc agcctctggg gcctggcctg cgtctgcgcc ctcggcttcc 60
acctggttgc ccttgcccag cttcttcaca ctctccagaa aggccttcca ggtctccggc 120
<210> 277
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 277
tatcaaggta cctgcagcca tgctgagcgg ggtcccagac cctggggggc tcctagcagg 60
cagcaaactt gcgctggatg cgcttgtacc ggagcagctc ctgctcactg actgagggtt 120
<210> 278
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 278
tagtgtgtac taaaatctct agtgttaact tatgtaacct cattttgttc atctccatca 60
taaacattgt atccttctaa tttaattagt tcaatgtttt ttggttcctg aaaaaataca 120
<210> 279
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 279
tgcagacagc catgcagcag taagcctttc aagggtcagg ccctttgtta aatgctcgct 60
gaccagcctg catggtgcag gaggctggct cacagaaacc gggaagccca ggaggtcccg 120
<210> 280
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 280
gccagagagc gagcagaggg ggcgggcagg ccacgaaaat gtcctcggcc gtggggcccc 60
gcggtcctcg cccacccacg gtgcctcccc ccatgcaaga gctgcccgac ctgagccacc 120
<210> 281
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 281
caaatcatct ctcttgagat agcctaaatg ataattgtac attttcagtt tttaatttcc 60
ttcccaatct tgatggttaa attcttcata ttctacggtc agtaattcag aatttctttt 120
<210> 282
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 282
aaatgtatac tctagtcagt catctcagtg ctacttcttc aatttcatct tctacagaat 60
cagctgcttt tactgctggt ttatcgtctg catgttttaa tcggtgccta tttggattaa 120
<210> 283
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 283
gcccagttca atttaatctc cttttgcttt tccatttttc tgcttttttc cattttctat 60
catgagttgt ttttctgatt tgctcacacc atttgctgaa ataccattca tggctgtttt 120
<210> 284
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 284
gcacccgagc gcctgcctgg tggcggcggc aacaatgcac ggagccgcca gagcgccagc 60
caccagcgtg agtgccgact gctgcatccc ggccggcttg cgcctcggac cggtgcctgg 120
<210> 285
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 285
tttctttcag gtggtgccca ggcaacatgg gtgactggag cgccttaggc aaactccttg 60
acaaggttca agcctactca actgctggag ggaaggtgtg gctgtcagta cttttcattt 120
<210> 286
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 286
gccgcggcgg cgagggcggg cgcgctcccc gggagggccc ggcggggaac ggccgcgatc 60
ggggccgcag ccacgctgcc gaggcgcccg gggacccgca ggcggccgcg tccttgctgg 120
<210> 287
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 287
ttagtatctg aaaattctga actctgaaga gaaattccat ttggtttgtc attttcacct 60
ctgtgcattt cggcagctct taacttttct tcaattcttt actggaaaga aaaatacaca 120
<210> 288
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 288
cggtgtgcgg tggagcggcg cggatgctgc ggacgccggg acgccacggc tacgccgccg 60
agttctcccc gtacctgccg ggccgcctgg cctgcgccac cgcgcagcac tacggcatcg 120
<210> 289
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 289
tcctggtgaa gcagtccctc acccctagtc agcccacacc cctagggcct aaagatgctg 60
aggtctgtat ggaattttct gaaacgccac aaaaagaaat gcatcttcct gggcacggtc 120
<210> 290
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 290
actaaatcaa acttatattc ttacgataaa tcccacctgg tcatgctgta taaacctttc 60
tatatgttgc tgaatttggt gtggtatcac cgtgttgagg attcttctgt gaattaattt 120
<210> 291
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 291
cggtgtgcgg tggagcggcg cggatgctgc ggacgccggg acgccacggc tacgccgccg 60
agttctcccc gtacctgccg ggccgcctgg cctgcgccac cgcgcagcac tacggcatcg 120
<210> 292
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 292
tttattatta tattttattc aggatgacaa gccatcagga ggtcaacaac acaagcacag 60
acagagggaa agaggccaaa ctgctgaatg tcagcggcct gtctggaggg gctgaggctt 120
<210> 293
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 293
aacattcccc tgaacttctc tcttgctgtt tttcttccta gttctgagaa atcgagaaac 60
atgataagga attggctgac tatttttatc ctttttcccc tgaagctcgt agagaaatgt 120
<210> 294
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 294
tttctaactg gaggccatct gggctgacca cccacaccct cactcctccc acccagagca 60
tgaagcacaa gaaagcatca ggttgctcct tgaatacctc cagcgacggg gagctcattc 120
<210> 295
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 295
cccgctccct gctctgagca cagcccaccc ctcagggcac cgactcgaac ttggggggcc 60
cacgcgccca gctgagcgcc ggcagcggcc gctcgtggtc tttgtccgac tcgggctggc 120
<210> 296
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 296
ggcttggaga aactcggggt tacggggaga accctagggg aggccggggt ctcagtcgct 60
cagcctgctc cgtctgtgtt cgcagaagcc ggcaatgacc gcctgcgccc gccgagcggg 120
<210> 297
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 297
atggggagag gagagacggg aagcgcgctc ctaaatagaa atgctgttct caatcagcac 60
cgggtccagg tagtagtagg ggatgggaag gcacttgttg cgctggcgga tgtcgtgtga 120
<210> 298
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 298
tctttcttct tgggtggtat ttgggggtgg ttagatggag acgctgttct caatgagggg 60
agggtccagg taggtgtagg gcagtgccag accctggttc cgctcctgga tgtcccttga 120
<210> 299
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 299
gcggcgccca gcgtcccgtg aggagagagg acacagggat cccggggagc ggccccagac 60
tcgtaaatta tggccgcatc tccgcacact ctctcctcac gcctcctgac aggtactgta 120
<210> 300
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 300
tccccagcgc ccccggaccg tgcagttctc tgcaggacca ggccatggag ctcgaagtcc 60
ggcgggtccg acaggcgttc ctgtccggcc ggtcgcgacc tctgcggttt cggctgcagc 120
<210> 301
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 301
cgttggggag gtcacagctc ccagcccaga actggagcct cctggggtca gggcagccgt 60
ggggtcaggg tgtcccgctg taggaatagt ctgctttatt gtgcatcacc agccggtcat 120
<210> 302
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 302
gcagagtgct gacagggcca gaggccagag gccgcagggc tataaagagg agggccacag 60
agcaagtggt accagatgga gacccaggct gagcagcagg agctgggtga gtaaggccct 120
<210> 303
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 303
cctccgccgc cccccggccg cggggaggac atggccgcgc acaggccggt ggaatgggtc 60
caggccgtgg tcagccgctt cgacgagcag gtaaccggcc cgtggcgggc gggaggtggg 120
<210> 304
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 304
tgattccctt tcagttctca ggggagtaga gtttaatcag atgttgtact tctgttggat 60
aacctgtgat gggacaagct tggtgactcc tcacataatg aaacacacag cggtaacaaa 120
<210> 305
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 305
acctcaccta ataaggctgt tactcccaat cccaaggata gacagtggga ggggtgaatt 60
cttcatactg agcttcaaaa gccacttggc ctgagtcttc agttaaggac gaagactgag 120
<210> 306
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 306
tgttcctaag gaaccaatca tggggcagat cttgtgaaat tttacattag ttcttcaatt 60
tcctgaactt gagatgagac cacctcacca ttcaccatct cctgcacaac tgtcttgatt 120
<210> 307
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 307
ggccagctgg tgggcaggag gctgtggtag aggcagctca gttctaggag ctctggccct 60
ggctgaagct ggatgagctc tgctccttga ggatgggccg ggtctgacgg ctcgaagagg 120
<210> 308
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 308
tgcctccctg cctcctgggt ggccggccgg ggtagctgag tcctcagcgg gtggtctggt 60
ggacctgctc gcgggaggag atgaccttgc catcctggac ctcttccaca atggtacgca 120
<210> 309
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 309
tgcctctact aggcgcctta gctcactatg gggaaccact tgactgagat ggcgcccact 60
gcctcctcct tcttgcccca cttccaagcc ctgcatgtcg tggtcattgg gctggactct 120
<210> 310
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 310
ccggcttgtc agctgtgaca tccaggtagg aagaagcggc accgtgtcct ctgggaagtc 60
tgtggtgggg gcttttactc tgaaccttgg gcctcatcct ctctgcctgg ggagagtttg 120
<210> 311
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 311
gcgtggagga gggggggcgg ctcagccccg cgcccgtgcg cgcgctcgcg gccgggttgc 60
agggctgggc gcgcgccccg cgtcccgggc aggaagatgg tggcgagcgc gcgagtgcag 120
<210> 312
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 312
taccacccag ctagcagaaa ggactcagca ctgccttcag ccttcactta ctcagaaata 60
actcatatct caacctcagc aagccccaag cagtagaatg aggcttccct ggaaagaggc 120
<210> 313
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 313
cccgcggcgg cctcctcggt gcgcgacccc cggctcagag gactctttgc tgtcccgcaa 60
gatgcggatg ctgctggcgc tcctggccct ctccgcggcg cggccatcgg ccagtgcagg 120
<210> 314
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 314
gtcgggaccg atgtcggctt gggctgctgc cagcctaagc agggccgctg cccgatgctt 60
gctggcacga ggccccgggg tcagggcggc tcctccgcgc gacccccggc cctcccaccc 120
<210> 315
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 315
tggggggctt ctcctcttgt ctcccactgg tgctctggct gtgaatccat ccaggggcca 60
ggatgacaat ccgacaccaa ggccagcagt acaggccgag gatggcattt ctccaaaagg 120
<210> 316
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 316
cagggctggc aactgtgagg acctcgagac cccaccatag gttgtggcaa cttgcaattc 60
attttggtct ggggagagga gcccccggtt atctgtggag gaagagacag aaagaaacag 120
<210> 317
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 317
gccccctctc cttccgccca gcacctccat gccctcggtg cacgaacgcg gcggcggcgg 60
ccagaagtcc cccgtggctt atgaatgaca cggacaggca agaaccagcc gctgccctga 120
<210> 318
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 318
catcccaggc ccctccgggg acccggccag cctgaagatg ccgacgaacg gcctgcacca 60
ggtgctgaag atccagtttg gcctcgtcaa cgacactgac cgctacctga cagctgagag 120
<210> 319
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 319
gcagggttta gagcacagtg ggcaggaggg ggagggtctg gacttcagca gggcctcgtg 60
ctagatgatg ccatattggg ccagctcgtg cagctccagg tggttgaagg cctcccaagg 120
<210> 320
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 320
aagaatgcag ggatgagatc agtagccatg aggcacatca agatgggctg caaatcagct 60
ctgagccccc taggaagcaa acaggagtga aacacgatca gtctccagca tgtgggaacc 120
<210> 321
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 321
cagtgctgac tttttgggat ggctctggaa ggatcacctt gccaaaacac aggtcaggct 60
gggtggtgtc accgcaggag ttggggcagt agcccccagg cacacacagg cagcaggagt 120
<210> 322
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 322
aagttttgac ccagcttagc agccgtagtc agccgggagt gctgtcgtgg gggattgtgg 60
gaaaagatgg cggctgccgc acaatcccgg gttgtccggg tcctgtcaat gtcacgtaag 120
<210> 323
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 323
ggccgagccg ggggaagggc tccggtgact taaggggagg gaatgctcct ctgccgtgct 60
caccgcgtgc tggggctgcg cggggctagg tatggtcggg ctgttttccc actgtccctt 120
<210> 324
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 324
ccctaagaac actccttcta ttcccatttc tgacatccta cccacaccca cattcatcga 60
ctaccatgtc ttcataatgt cgtagaatga cattgtcatt attgtcctgg tagagcatgg 120
<210> 325
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 325
tcactgtcta aggaggctga aagggaaaca tcagccgccc ggggggagtt aaaatgagtg 60
acaacactac tctgcctgct ccagcttcaa accagggtcc taccacccca cgcaaaggcc 120
<210> 326
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 326
gtattatcac agcattcagt ttacaaccta tcttcattca tgtttaatta ctggcaatat 60
cttcccccag atctcctgtg tcagagatct gaccatcttg caaattgcga gccttccaaa 120
<210> 327
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 327
tgagagattg ctgatgtgtg gggacttgac tgagcagaca acagggatgt ttctttttct 60
cggcgggttt cagtggcctt cgtcactact cgatgagcag gcccagtcat agatgaccaa 120
<210> 328
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 328
ggtcctttgg gtttgcaacg gattgtgttg tttctgggtt caggttttta caagaagcag 60
accggcccta tgtccacacc gaattcctgc tcgggccctc ctatgtccat gggtgcaatg 120
<210> 329
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 329
gagctgtggg actgagagct ggcggcagca cttcagtgct tgtagctctt cctgctggag 60
gaggaggtgg tggtgtactt gatggtggaa ctgccgcctc caacagagct gaggccaccc 120
<210> 330
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 330
ggactgtggg accgagagct agcagacgca ctttagtgct tatagctctt cctgctggag 60
gaggaggtgg tggtgtactt gatggtggaa ctgccgcctc caacagagct gaggccaccc 120
<210> 331
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 331
cttggggagc atggggtggc gctgggggtg ttgccaatat gggggcgtgg ggagcggagg 60
ggaggcgctg gagtggctgc gatgctgatg cgtgctcttt atctggagta gcgctgggag 120
<210> 332
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 332
cctgtaggtc acttgggctc cagctatgtg gctgcctctt ctgctgggtg ccttactctg 60
ggcagtgctg tggttgctca gggaccggca gagcctgccc gccagcaatg cctttgtctt 120
<210> 333
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 333
tctactccct ctattcagct ggagcttcta cagggcctgg gtgttcagtt caacaggttc 60
ccctgtgacc tctggttgtc cattggagac atcgggtttg gcacccttga ggacagacag 120
<210> 334
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 334
atgaaaagtt tggttaatta aaaacttctg atgttatagt tcatcttctg aatcttgatt 60
gtgaactttc tgagggtgtc tcttaacagt gattaccatg tcaatggtta atatttttgt 120
<210> 335
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 335
ctgtgcccca ggagctacgc gacagtcttc caggattatg gagtttctga gcgagaagtt 60
tgccctcaag agccctccga gtaaaaacag tgacttttac atgggcgcag gaggtcctct 120
<210> 336
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 336
aaatagttaa atgaaacaaa gtttataggt gacctttagt aaatggggaa attaacagga 60
ctttcttctt catcttcaaa ctcttcagaa gcagcaacag ggctagttaa ttcaactccc 120
<210> 337
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 337
gattaataca gagctagtgt ttggcatttg actttatctc aaatgagcta actgctcaat 60
gaattacaga agactcatac tctttttatt ttttcctgga aattaaaaaa gaaaagcttt 120
<210> 338
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 338
tggactgagg acctccgttt tttgctggct ggcattgaag attatcttca gttcctgcca 60
cataggccac tccatagagc agctcagagc agggcaccta gatccatcaa aaccaggaag 120
<210> 339
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 339
agagatcttt gctcttctca ttggctttcc cttttaggat tcccaggagc catgttgtca 60
gaagtcctac tggtgtctgc tccggggaaa gtcatccttc atggagaaca tgccgtggta 120
<210> 340
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 340
cgctgtccat gtcgcgggcc tcgctgggac tccctgggag atgaggccgc gaggtctccc 60
gccgctcctg gtggtgctcc tgggctgctg ggcctccgtg agcgcccaga ccgatgccac 120
<210> 341
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 341
aacccaggtc ctcagccagc agagccacgt tccttatgag caccgtgggt ttatttcatt 60
ttcctacacc actgacccga atatgcccgg cgccatgggg actccggctt tgggagaagc 120
<210> 342
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 342
agccagcccc aagcccagcg ccgccgccgc ccgcggagcc ccgagcggag ccggaggcgg 60
cgatggaggg agtcagcagc caccggaccc tgtcttacag ccgctggagc tatgacaggt 120
<210> 343
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 343
gaaaatccca ccgccaaaat tgagcccagc agctggagcg gcagtgagag ccctgccgaa 60
aacatggaaa ggatgagtga ctctgcagat aagccaattg acaatgatgc agaaggggtc 120
<210> 344
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 344
agtgtggcct ctctcaaggc tgatccgagg ctttgtgttc acgaggtggg gccggagctc 60
actgtttcct aacggtgaat ttggtttccc cttttggact tcagaagaag gtcctgcagg 120
<210> 345
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 345
ggaaaaagat tcacacatca gtctccacag cctttgcatt agaacagttg tttttatctg 60
tctcactgtc atcccccttt ccctgacact tctcctcctt tgcacacaga gattccttaa 120
<210> 346
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 346
gtgcacatag ctataccaga aaaatacagc agaaaatccg atggttctga taggagttaa 60
ttgtggagat gtgccagaga cagcagcttc gtggatggtg acaccacaat gtctgtggct 120
<210> 347
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 347
tcccagagtt cctattgggt taacactgga ggctctaaga tggcggcagt ggcaacctgc 60
ggtagtgttg ccgcgagtac tgggtctgca gtggcgacag ccagcaagag caacgtcacc 120
<210> 348
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 348
tttttttttt ttttttttta ctgtaatctt ggccagtatt gagacatatc aggttcactt 60
ccgggaactt gaactggaac tgacacacca ggggaaatga gtcctagaag tggatgaaag 120
<210> 349
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 349
agtgacaagg tgcgggctgc agtggcatcg tcacaggaga gcgcagtcaa gggtgtcctg 60
ggaggaacgc tggtggcgac cagggctgtg tgtggggcct ggccggtagg cacggagagg 120
<210> 350
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 350
ttggggctcc gtgtcctgct gtctttccgt ccgctgccta gtctgcatct gagtaacatg 60
gcggcggcgg cggtagccag gctgtggtgg cgcgggatct tgggggcctc ggcgctgacc 120
<210> 351
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 351
cggttttctg ggaactgtgg gatgtgccct tgggggcccg agaaaacaga aggaagatgc 60
tccagaccag taactacagc ctggtgctct ctctgcagtt cctgctgctg tcctatgacc 120
<210> 352
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 352
ggccggaaac ccaggccgct tcgtccctgt ttccggcagc gccgcgctgc tccgggagcc 60
gctgtggcag cgtatgctgc cacggggact gaagatggcg ccgcgaggtg agattccgga 120
<210> 353
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 353
tctaccagga cccaagccca cctgctgcag cccactgcct ggccatgacc atcacttaca 60
caagccaagt ggctaatgcc cgcttaggct ccttctcccg cctgctgctg tgctggcggg 120
<210> 354
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 354
tgacacctct gcattccctt gggcagagat gggagatggc gccggtgttg cccctggtgc 60
tgcccctgca gccccgcatc cgcctggcac aagggctctg gctcctctcc tggctgctgg 120
<210> 355
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 355
ctcagtgatc accgaggtct gggccgaggg cggcgtcggc ggcgtcagcg gcggcgctgg 60
ggaacgcagg ccccgtgcgg gcggctgcgc gcgaagccgg ctttgcagac gcagcggaag 120
<210> 356
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 356
cctgtgctcg ggcactattg ggcgttacgc gagggcgggg ccggttgcca ggacgacgcc 60
tgcgaagatg gccgctgcgt cctcatcgga ttccgacgcc tgcggagctg agaggtgaag 120
<210> 357
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 357
ctcagcgccc ggccccggga tgacggcggc ccaggccgcg ggtgaggagg cgccaccagg 60
cgtgcggtcc gtcaaggtgg tcctggtggg cgacggcggc tgcgggaaga cgtcgctgct 120
<210> 358
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 358
gtctctgagc cctgttatcc ctcccccatg accacagagc aggcaagggg gcagcagggc 60
ccaaatctgg ccattggccg tcagaagccc cctgcggggg ttgtgactcc caagagtgat 120
<210> 359
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 359
ctcactcagg atccgcggcc tccgtgtcct tcaggtgcag cggggagccg gctctcaggg 60
cgcatgcgcc gcccgctctg cgccaccggc ctagttaggg tcggagatca ctgggaaggt 120
<210> 360
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 360
gctggcgctg tgcggctgcc cggcccccgc cgcgggtagg tgggcgcagg ccggccgggg 60
gccgcgggtt gctcggacaa tggcccgggc ggccccgcgg ccaagtgcgc gggcgccgcc 120
<210> 361
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 361
ctgggggcag gctcggagcc tccagaggcc ggcagctaga agagattggt cttcagggcg 60
gggccgggct tccggtgaaa ggaggacaga accccggaga agggcccggg gccggactcc 120
<210> 362
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 362
caggtgttcc ccctcccctc cctggggcag cagccaccat gttctcgtgt gtgaagccct 60
atgaggacca gaactactca gccctgaggc gggactgccg gcgcaggaag gtgctcttcg 120
<210> 363
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 363
tgcccagaag catgacatgg tctctctccc tgcagaactg tgcctggccc agtgggcagc 60
aggagctcct gacttgggac catggtgatt cttcagcagg tcagtgttcc cacctctttg 120
<210> 364
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 364
gtataaattc ttgagaaatg atatcttcat gttttttaag gctcaaaatg gaaaatcata 60
aatccaataa taaggaaaac ataacaattg ttgatatatc cagaaaaatt aaccagcttc 120
<210> 365
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 365
gaagaaagca tggaggctga ctagccttat accacagtct cactgccttt tccaggcttc 60
acccaaccat ttcctctctt ctctctcttt acctttccag aattcaccaa tctgttggaa 120
<210> 366
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 366
ctagaggaag aatgctcctg gctgttttgt actgcctgct gtggagtttc cagacctccg 60
ctggccattt ccctagagcc tgtgtctcct ctaagaacct gatggagaag gaatgctgtc 120
<210> 367
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 367
cagttcttca ggaaaacacc ttctttggac tcacaccatg tgttttccat tcaaattagt 60
aatgttcaat ttttcctgca gttgaaccag ctattagctt tctggagagt caaaattctc 120
<210> 368
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 368
caattcaaga aagggcatct atttcttggg ggctgcggtg acagcaggct tctcttcacg 60
ggtgatggga atggtgcgct cagggccaga gacctgtttc cttggtccat tcacagtgag 120
<210> 369
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 369
accgggaagg ggggcggcgg cgggcgcctc actgggtgtt gagcttctta gtgtactcct 60
cgagagcgct caggaagctg accttgaagc tctccagcac gggcagcagg ccttggcgga 120
<210> 370
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 370
tcttttggtg ggggcctctg ggatctaggt gtttgagccc agatgagtca tggctggacg 60
acccctccgc ataggagatc agctggttct ggaagaagat tatgatgaga cctacattcc 120
<210> 371
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 371
tgcagtgggc actaagcaaa gactggggag ctgtgccagt cggagtacac cagaaatccg 60
gagaaggtgc tgtctgtctt gatgctggca tagatgccaa tgtagtcacc cacacccacc 120
<210> 372
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 372
gaggacgggc aggaagaggg caggggccgg cttcagggtc agggctgggc acaagcttcc 60
aggtcagctg cctctggcag cctgttgcag ttgaagggcc agggggtgcc cagtagtgcc 120
<210> 373
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 373
tagcgaggca gcagtgcagc tttcagaggg tccgggctca gaggggttat gattcggagg 60
gttctgccgc acggcatggg ccggggcctc ttgacccgga ggccaggcac gcgcagagga 120
<210> 374
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 374
gcttcctctg tcaccatggt gccggctcgg ctgggcccgg cggtcgccat ggtaactggg 60
gcgggtcgca gggtcctggc aggctgggcg catgcgcgcg gggactacaa gccgcgccgc 120
<210> 375
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 375
acttccgttg agcatctaga cgtttccttg gctcttctgg cgccaaaatg tcgttcgtgg 60
caggggttat tcggcggctg gacgagacag tggtgaaccg catcgcggcg ggggaagtta 120
<210> 376
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 376
aaaatgcttt tcaaaataca tgcctgaggt gagactgctt gtatgtttgc atgtggtagc 60
ttccagagga aatcttcagt tttaatcttc aggttcatat ctggagatat aaattaagaa 120
<210> 377
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 377
gaggaagagc agtgtttcct gtggatgaag tgaagttact gaggtgctga agctacagga 60
aatcacttcc atcgtagatc acaggccgat caaccgtcaa gtgataaaat acctttttag 120
<210> 378
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 378
acttcctgcc tgaaaatggg ccctggagct cgcagacagg gcaggattgt gcagggaagg 60
cctgagatgt gcttctgccc accccctacc ccactccctc cccttcggat cttaacactg 120
<210> 379
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 379
gcaccaaggg gagggacagt ggggttctgc tgagaccccg actcctccag gggatgctga 60
atctcagctc attatctggg cctcagtcct gggcagtacc tggtgaggac aggttggaaa 120
<210> 380
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 380
ttctggggat cccctccatc cagaagaacc acctgctcac tctgtccctt cgcctgctgc 60
tgggaccatg ggggctgggg ccagtgctga ggagaagcac tccagggagc tggaaaagaa 120
<210> 381
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 381
ccgcgggggc catggatggt gaaacagcag aggagcaggg gggccctgtg cccccgccag 60
ttgcacccgg cggacccggc ttgggcggtg ctccgggggg gcggcgggag cccaagaagt 120
<210> 382
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 382
gatgagctgg ggaatcagag ggaggcagca gctaaagtgg caatgcttag actctttcgt 60
tttgttacaa aggggcgacc gcgtggagtt catcagtgta gctgcggcaa ccaagatctg 120
<210> 383
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 383
ctgtcagcgt gccccacccg gtccgcgggc cgcgcacgcc gccggaactc cctggcgcct 60
ccttaaaaaa cggcccccgc gcgactcttt cccccttttt tttgttacat tgtaggagcg 120
<210> 384
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 384
gctaccttcc ctccgccccc gggctctgtt ctcactttcc agcagtggaa ggacgggaag 60
cgggagccat gcagtccgaa tcggggatcg tgccggattt cgaagtcggg gaggagtttc 120
<210> 385
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 385
gagcggggtg ccggtgtccg ctccgggctc ggatgggaag tggtgggagg agcgacccgg 60
gatgttcagt ctgatggcca gttgctgcgg ctggttcaag cggtggcggg agcctgtcag 120
<210> 386
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 386
ttgggtaata ttttattttt tcttaattgc agctggtttg taaatatttg aatcacatta 60
tgggattgct agacagactt tcagtcttgc ttggcctgaa gaagaaggag gttcatgttt 120
<210> 387
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 387
aatccaggtt aattatatga cataagtaac aagtaatctc catcttctcc aggcttctaa 60
tcagtttcag aacaggagtt ttggttaaac ctcttccctg ccatgaaaac acaaagagca 120
<210> 388
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 388
aatatgagat ttgtgtcaat tcttagggtt actcactaag gtggtttggt tccaccaatg 60
aataaatttt ctaattctat actaagctca tcctttggaa catcaatctc atctccagat 120
<210> 389
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 389
tcccttgccc tggagagacg gcagaaccat ggcattttat agctgctgct gggtcctctt 60
ggcactcacc tggcacacct ctgcctacgg gccagaccag cgagcccaaa agaaggggga 120
<210> 390
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 390
agccacgggt cagccacaag ggccacagcc atgaatggca cagaaggccc taacttctac 60
gtgcccttct ccaatgcgac gggtgtggta cgcagcccct tcgagtaccc acagtactac 120
<210> 391
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 391
tacatttgca aagaattcta actgctgcta ttacctttgt ccttgctgaa ggaaaacatt 60
aggagaatga gctgttttta agctaaacgt gtctccactt gatgaatggc gtgaaggagc 120
<210> 392
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 392
tgggcaccac agaggcagaa ggggtgatga gtgagaggcg agtggtagtg gacttgccca 60
ccagtgccag ctccagcatg cccctccaga ggcgcagggc gtccttcagg gggccacggt 120
<210> 393
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 393
tgtgaggtca ggctggcggc ggcgtcgtcg gctgcgaccg gggccggcgt cgcgcgtccc 60
tgcatcctcg catccgtctg caccggcatg cggtgggctc tcagagatcg aggcgcgaat 120
<210> 394
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 394
caacgtctcc gccgtcggct ccgcggcgcc gccatggccg acgtggaaga cggagaggaa 60
acctgcgccc tggcctctca ctccgggagc tcaggctcca agtcgggagg cgacaagatg 120
<210> 395
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 395
tcccagcagt tctgacactt cagacttcat tgcacagcct agtttccctt tagaagaagg 60
gaacggtccc attccagttg atggtggcct cgctccactt ggttctgatt gccatctcca 120
<210> 396
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 396
acaattacct tgctagaatg tccagtagca tatatattaa ttgttccttt tgcatttcca 60
cgaagatgat ccttcatcct gtaacttttt aaattttgga ccaagaaaga accaccaacc 120
<210> 397
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 397
acccacttgt gtgtttctcc tactctggag agtaatctaa ctcatcattt gcaatcaggg 60
cctctgagtt ctgtttgtgt ttgcttttgc tcttcgcttt gctacttgtc cggccgtttg 120
<210> 398
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 398
ccggcgggcc gggcccgcgc acagcgcccg catgtacaac atgatggaga cggagctgaa 60
gccgccgggc ccgcagcaaa cttcgggggg cggcggcggc aactccaccg cggcggcggc 120
<210> 399
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 399
ccccattcat gtcattactc cacaatgcgg cggaaagact ggacagctgg ggaggctgca 60
ccccaatcga tgggcttccg gtactccttc ttgtccagga ggtactgcct gccacggtag 120
<210> 400
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 400
tatgtgtata ttttatataa ttgttctccg ttcgttgata tcaaagacag ttgaaggaaa 60
tgaattttga aacttcacgg tgtgccaccc tacagtactg ccctgaccct tacatccagc 120
<210> 401
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 401
ggagccgggc tggggctcac acgggggctc ccgcgtggcc gtctcggcgc ctgcgtgacc 60
tccccgccgg cgggatgtgg cgactacgtc gggccgctgt ggcctggtaa gtgcaggctc 120
<210> 402
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 402
tgctgggatt ccaggtgaga gctgccgcgc cgaggcagag gtcttggtct tgtaatgagg 60
ccgagggtaa agagtggtcg tgcttccggc ttcctgcaca caaaggcaat ggagttcatt 120
<210> 403
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 403
cctcggccgc cgcaatggcg acggtcgggg agcgcaggcc tctgcccagt cctgaagtga 60
tgctgggaca gtcgtggaat ctgtgggttg aggcttccaa acttcctggg aaggacggtg 120
<210> 404
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 404
tatgctgtat aaaagtactt taattatctt tcaggttggc aaataaacaa gataaagaag 60
gagctttagg agaagctgat gtcattgaat gtctatctct ggaaaaattg gtcaatgagc 120
<210> 405
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 405
tgagtctgag gaatatgggg tctggacccc cagcacatcc ccttcatagg tctccatggg 60
ttgggagagc agggtcaccc tcatttttgt tgcaggattc acttgcagtg tgaatttcaa 120
<210> 406
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 406
ggaaatttaa aattgtttac agtcaacact gtttccagcc atgggtttgt ctccatctgc 60
tcctgctgtt gcagttcagg cctcaaatgc ttcagcgtcc ccaccttcag gatgcccgat 120
<210> 407
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 407
tcttggcccc accgcccggg ctgggcacta aactcgggcc gcggcggggc gagcgaggcg 60
ggctccggag ggagctgacg cctgatgatg gcgcaggtag acacacctgc cccttctcgg 120
<210> 408
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 408
cagaaagtcg ccgcctggct gcgcgggatg cctttcgcca agcggatcgt ggagccgcaa 60
tggctgtgca ggcagcggcg ccctgcgccc ggcccagcag tggacgcgag cggaggcagc 120
<210> 409
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 409
tgagaagttc ttttggtgcc atgttttgtg gcttgcatca aaagaggagt ttgtcttcat 60
gaagattcct aacattggta atgtgatgaa taaatttgag atccttgggg ttgtaggtga 120
<210> 410
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 410
tctgtgccag tcaccccctg gcaggcgccg agctgaggca ggcatcaggc acacttgctg 60
acgcactcca gcagctccat ccggatggca atgcggacgt gccagcccag cgggatgagg 120
<210> 411
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 411
gcaagtcatg cgcactctgc tgctcttttg tttctcccca ctaactagac ggtggatgtg 60
atggcagaag acagagacac cattctggac cccaagatag acgcttcatg ttaaggacga 120
<210> 412
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 412
ctattttttt ttaaaagcac atgaaaaaaa atccccgggc cctgcttacc caaagccttg 60
actgttgagc cgggccttgt ttgcagtcca cgaagaatac cagcccacat actgctccgg 120
<210> 413
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 413
cattccactg ccctctgaat attttccagg agaatacttc tgctgtggtg acccaaagaa 60
gcctcacact ccgtgcgtcc caaaccgagc ccttcatcgt ccaatctcca gtcctgctcc 120
<210> 414
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 414
tgggaaaaag gaagagaaat ggggtgttcc caatgactgt tccatcccaa ggacagggga 60
tactcacctg gtatacagtc caaggaggtg gcacctgcag accacagagc attgggtcct 120
<210> 415
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 415
gtctgctggg gcacctgaag gagacttggg ggcacccgcg tcgtgcctcc tgggttgtga 60
ggagtcgccg ctgccgccac tgcctgtgct tcatgaggaa gatgctcgcc gccgtctccc 120
<210> 416
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 416
gcagggagga ggacgccggg gctcgccttc cctcctctgc cgccgctgcc gccatgattc 60
cggtgtcgct ggtggtggtg gtggtgggtg gctggactgt cgtctacctg accgacttgg 120
<210> 417
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 417
cctactgttc aagtacaggg gcctggtccg caaagggaag aaaagcaaaa gacgaaaatg 60
gctaaattcg tgatccgccc agccactgcc gccgactgca gtgacatact gcggctgatc 120
<210> 418
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 418
atattataaa aaccatgcgg gaatcaggag ttgtaaaaca tttattctgc tccttcttca 60
tctgtcatga ctgatactaa ggactccatc gctctgccca aatcatctgc catgtggaaa 120
<210> 419
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 419
aaaacataaa tatatattta tagtattgta caggattttg atcttctctc tgtatttgtt 60
ggtgggatat tctgatgatt ctgactcatg tggttctggt cggcatcttt attatcactt 120
<210> 420
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 420
gtagctttga tggtagttca gcttcaccag tcctcttctg cttaaatcat ttctacaggt 60
ctgttttact ggtcttgtcg tctgtttttt tcccttcaac actcccccta gctatttgag 120
<210> 421
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 421
gcactgggtg acctgtcctt tctcccctca ggtaggggtc ccacggctgg gtggtcctaa 60
gccactgggt ggatgaaagg ccgagggatg ttggtcctgc ttctgcatgg tgagttctcc 120
<210> 422
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 422
tcccagttat gctcagatat ctggggagag gcctaataga cccaggagac agggacccac 60
tgtggggctg tgcacacgag ctcaacagct tcctccagat gtctgattgt ctcatcaatt 120
<210> 423
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 423
ttcacttttt gatctccaca gaagacaatg agaagtcata ccataacaat gacgacaact 60
tcagtcagca gctggcctta ctcctcccac agaatgcgct ttataaccaa tcatagcgac 120
<210> 424
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 424
gttatatcag tatttcttca caaacattaa agttagtgat aacaaatgac ttaccttcac 60
atccctgtga attatgttat tatcatggca gtagcgtaga gcttccagta tctgtctcat 120
<210> 425
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 425
gttgtcccat ccagaagcca cacacagcag cgggcctcag gaattgcatt cctcgcagtg 60
acaggagagg atgtaccggt aggtggcagt gagtcgcatg ccccctgagc atcgcagccg 120
<210> 426
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 426
gggttcacgc cacactctag gaagtgcctg agctagtgag ctggccaacg agctccgcgg 60
gctgggacca tgggctgctt cttctccaag agacggaagg ctgacaagga gtcgcggccc 120
<210> 427
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 427
cctgggcctg ggagaggaac agggccctgg agcacctggt ttcggggaca gtagggttgg 60
tgggagttgc gatgatattg ccacctacct cttacatctt ttagtgcgta tgttctgtcc 120
<210> 428
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 428
ggaggtttat tgagcagttg gggaggggtg ggaattcaga gggcgtggac gcaggccatc 60
tcgtctccca agtcctcctc atcgaagcgg gagaggatgg actcctcgtc gctatagaga 120
<210> 429
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 429
cctggccctg ctggtgcctc cggcgctacg ggctgggcaa gatggcggcc ttcgggatct 60
tgagctacga acaccggccc ctgaagcggc cgcggctggg gcctcccgat gtttaccctc 120
<210> 430
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 430
gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagctgta tttccaaaat 60
gtcgctttct aacaagctga cgctggacaa gctggacgtt aaagggaagc gggtcgttat 120
<210> 431
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 431
ggtatccagt ttggtgtata tccattattc agaggactcc tctaggtttc caaggtttgt 60
gctttccttg aaagtctccg agttagcctc tggtatggca ctggattctg aagcctctgg 120
<210> 432
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 432
cttgaactac cttccttttc caaagaggta atgctgagat cagtcagaaa ggctttctga 60
gaaaactggc ttggatttct gggtctgttc cagtcgattc aagatgaact ggcttgtatc 120
<210> 433
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 433
gacaggaagt agtagttggc aataaaataa acattttaaa gtaagtcttt taatgacatc 60
tgcattgtat tttctagctg aagcaaaaca gtgcctgtgg gatttatgtg acttcttaac 120
<210> 434
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 434
tccagctgca gcttgagttt tcttgcttta ccctatccaa tactgtctgt cttgcctaac 60
agtggccctt ttcagatctc tccaggtaca aaaccttgac taaatcttca agctctgttc 120
<210> 435
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 435
ggggacgcgg gagccagtgt cgtcggatcg gcccgcagtc cgctgtcctg ctgagcccgg 60
aggccgcctg gatggagccg ccgctcccgg tcggagccca gccgcttgcc gtatccgccg 120
<210> 436
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 436
gaaatgtcca taggttcaca cttttaagct aaaaagtaat tacatggttt tagtgaagtc 60
ctgtcttcag cagttggtgt gttgaaataa gcatcttcat cttcttcatc taactgtaga 120
<210> 437
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 437
gcgccgtgac cctcaggtga tgcgccaggg ccggctgccg tcggggacag ggctttccat 60
agccatggcc cagcagtgga gcctccaaag gctcgcaggc cgccatccgc aggacagcta 120
<210> 438
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 438
gcaccgtgac cctcaggtga cgcaccaggg ccggctgccg tcggggacag ggctttccat 60
agccatggcc cagcagtgga gcctccaaag gctcgcaggc cgccatccgc aggacagcta 120
<210> 439
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 439
cttggtctgg cctccctcgg cccggtcctg gcactggccg tgctagctgg ccgtgcttct 60
ccgcgggata cagccttcca tctccagcgc ctcgggccag ccttcgtact tgttgctgtg 120
<210> 440
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 440
atcatcatga cacagcaaga atcttatcag accaatgggg aagtcagccc agaaatgttc 60
tgaggaaagg ggaggcgggg gccgctgggt catccctccc acaggcccat cattgggatg 120
<210> 441
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 441
ctaagaaaaa gatcactaca cagctgtctg gaggcaggca cccgggcact agatggccaa 60
gtcctccggt ctggcccgcc cgctgctggc cctgctggcc ttgctggccc gacctgggtc 120
<210> 442
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 442
tctaacaact gggcaatgcg ttaaactggc ttttttgact tcccagaaca atatctaatt 60
agcaaataac acaattcagt gacattcagc aggatgcaaa ttccagacac tgcaatcatg 120
<210> 443
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 443
tatctacatg accttgattt aagaggtgta gagtgtttct tttattcaga atccatgagg 60
aaagtttcga agtccagatc caagtcggaa acgagggcac ttacgaagtc atcaacagaa 120
<210> 444
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 444
cccgcgcgtc tcccttcccc gcgcccaggt agggcgctca gcctccgccg ccaggatgcg 60
gccgcccgcc tgctggtggc tgctcgcgcc gccggcgctg ctcgcgctcc tcacctgctc 120
<210> 445
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 445
gggagcccgc agcccgcgcc ccgagcccgc cgccgccctt cgagggcgcc ccaggccgcg 60
ccatggtgaa ggtgacgttc aactccgctc tggcccagaa ggaggccaag aaggacgagc 120
<210> 446
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 446
gtttttctca ggggacgttg aaattatttt tgtaacggga gtcgggagag gacggggcgt 60
gccccgacgt gcgcgcgcgt cgtcctcccc ggcgctcctc cacagctcgc tggctcccgc 120
<210> 447
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 447
gaagctccgg gatggatttc gggtctttgg agaccgtggt ggccaactct gccttcatcg 60
ccgcccgagg cagctttgac ggcagcagct cccaaccctc ccgggacaag aagtacctgg 120
<210> 448
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 448
tttatttcag tgtcctctgg gatctcttac agcttgggaa cagagatcat gtcacatctg 60
gtggacccta catcaggaga cttgccagtt agagacatag atgctatacc tctggtgcta 120
<210> 449
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 449
gtgctctgaa cggctcagag gaagaggtgg gaggggtccc cggacccggg gccgctcgag 60
gttagccggt cacagcgagc cttgtagaga tcgcgctccc gggccaggcg ggccacctcg 120
<210> 450
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 450
cttggcactt aaggaagaaa ggaccaacaa aggtagcatt aggaagacaa gcccagattt 60
atcaggatac ccactcagcc aactgcttta gtgcttcttc atcttcatcc actttgggag 120
<210> 451
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 451
tcatctgact ccagtcccat tgctcctggc acggggctaa gctccacctc gagatgccat 60
agggatggtc tctcctaagt gactgtcacc tccagcgtct gagaagaagc ccccatcccc 120
<210> 452
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 452
tatcctcaag gactgcctga tctcagcggc acccacatcc ctctactacc atctcctgat 60
aatttttcag taccacctta tcatactcat ccaggtacag catggagatg gcactcagtt 120
<210> 453
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 453
aaaagaggtt ctacaggtct tcacaaaacc tggaatttcc acgaggatgt ctgatcttta 60
taatccaagc agtcagcatt gaagttaagc ttccaggagg cagtttggtc cttataatcc 120
<210> 454
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 454
ccggcactgc ctcgatgttc cagctgccca tcttgaattt cagcccccag caagtggccg 60
gggtatgtga gaccctggaa gagagcggcg atgtggagcg cctgggtcgc ttcctctggt 120
<210> 455
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 455
gaatcatttt gacaagaagt actgtgtagt ctgctgcagt cttctcttgg gtccaactgg 60
cactgcctgt tagtctattg ggaggcccag caggtcacaa ctgacgtccc acagccgcct 120
<210> 456
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 456
ctggggttga agctggagca gcagcaaaag caacagcagc tacagaagtt ggaacgatgc 60
tggtcacctt gggactgctc acctccttct tctcgttcct gtatatggta gctccatcca 120
<210> 457
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 457
tggccccact gcccaaggtc acttcctgga gcctgggcca tgggcacccc gggggtggct 60
tgtcagaagc cactgggcac agatgtcttg cactactgca tccccgctgc tcttggcggc 120
<210> 458
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 458
cgaaccccgc tcgctgccgg ctgcccagcc tggctggcac catgctgccc gcgcgctgcg 60
cccgcctgct cacgccccac ttgctgctgg tgttggtgca gctgtcccct gctcgcggcc 120
<210> 459
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 459
ctcagcccct ccaaagaaca gggagatgac ggggaaagca ggggaagcgc tgagcaagcc 60
caaatccgag acagtggcca agagtacctc ggggggcgcc ccggccaggt gcactgggtt 120
<210> 460
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 460
gtgactggag gccatttcca ggtagttgcc acactgaccc ctgactgcta ctccttggca 60
gtgcagtggg ggggccctgc ctcagccaca tatcccgggg agcagtggca gcggtaggag 120
<210> 461
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 461
ggaaaggtct ctgaggcccc cgtctgctga ctgcatgaca aaccctaaag gaaatgccaa 60
tcgtgatggc ccgggacctg gaggaaacag catcatcctc agaggatgag gaggtcataa 120
<210> 462
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 462
gaccgaaggg gatacagcgt gtccctgcgg cggctgcaag aggactaagc atggatggca 60
gccggagagg taaagggcag ctgtcagggg ctaccgccgc ctcgcccctc gctccaggcg 120
<210> 463
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 463
ctcttcactc cacgcccacc tctctcctgg agcgctgggc cttcgctggc cgcaccggca 60
gccatgagct cggagatgga gccgctgctc ctggcctgga gctattttag gcgcaggaag 120
<210> 464
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 464
ctcaggaaag cgaaggagaa gtgggcaagg gtggcgggcc cgctccgcac cgcccagcgc 60
tccggacttc acttctggtc ccgctcctgg gcggccacga agttcagcaa gtcaatggcg 120
<210> 465
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 465
aggtccccgt ggtaccaaag ctgaacatgg acgtgaccat ccagcacccc tggttcaagc 60
gcaccctggg gcccttctac cccagccggc tgttcgacca gtttttcggc gagggccttt 120
<210> 466
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 466
gctggggaag acgctggggt ccccgtggag gctggagcgg cgttcaggtc ctgcggtcac 60
tcggcgtgct cctgcacctc ttccccacgc agggcctgca gccggctgcc cacagtctgc 120
<210> 467
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 467
tgcccggcct cccagcggcg gcggctgcag cgcggcggtc cgagcgggtg caccgcggcg 60
gaggaggcag catcccgcgg cgctgacggt cctggggaga gcatggcgcc gaggtgaggc 120
<210> 468
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 468
cgtctgagga cctgggcctt ggacccggac tcgttatgaa gagcgattct tcgacctctg 60
cagcccccct cagggggctc gggggacccc tgcgcagcag cgagccggtg cgcgcggtcc 120
<210> 469
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 469
caggaccagg ggacccagat ctggagcttt gatgaggaag ctgctcacaa atctgcctgc 60
agctgcagtc ttgagtgccc aggtgtacag tgctgtgctc cagggccttt gggaagagaa 120
<210> 470
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 470
ggatccagtg aaccattttc ttttggtttg aagccagagg tgttcctggg gagatggcgg 60
aacagcacgg agcacccgaa caggctgcag ctggcaagag ccatggagac cttgggggca 120
<210> 471
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 471
cctccaggtc ctcactgctg cttcccatgt cttccaggtc attcctgcac aggacagtcc 60
accatggcct cagatcacca gacccaggcg ggcaagccac agtccctcaa ccccaaggtg 120
<210> 472
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 472
gggcaaggta gcagagtgag gtgtggactc acttgggggg ctctgtggcc aggacaggga 60
aggacccctc gaggtgccac tgcttgtcac gcaggcgacg cagggactgc atctgtggct 120
<210> 473
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 473
gcataaagag gagagcagag ggtcagggtg gaagattata atgttcttct cttctgcagg 60
aaggggccac aatgaccctg caatgcacaa agtcagcggg accctggaag gtaggaagag 120
<210> 474
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 474
ggggctaaag ggctcagagt gcaggccgtg gggcgcgagg gtcccgggcc tgagccccgc 60
gccatggccg gggccatcgc ttcccgcatg agcttcagct ctctcaagag gaagcaaccc 120
<210> 475
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 475
gcgcgcacgg caactttgga gaggcgagca gcagccccgg cagcggcggc agcagcggca 60
atgacccctt ggctcgggct catcgtgctc ctgggcagct ggagcctggg ggactggggc 120
<210> 476
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 476
tctgtcttct ccggttgtgc agcacgatct gctccagctc gtcatgccag ggcccggaaa 60
acctccacct tctcccggta cagctggtgg aactgcttgg caaggcagtg gaaaggggct 120
<210> 477
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 477
ggtccgagaa cacctttatt gtgcacgtcc cccgcagagc agcctcaggc gtcctggtag 60
tagttgttga agttgatgcg cagcagaaag tcctccaggt ggggctggta gccgcggttc 120
<210> 478
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 478
ggggcccaga ctgcagtggc tcaagacagg acactgcgga aagccgccat gtgccgccgc 60
acactgtctg agatctgctc agccgatctg ctccggccgt agtaatccgt gaagaggccg 120
<210> 479
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 479
cgattgtggt cagacgcagc acctacttct aatctgagga cccgcgattt acacttttgc 60
tgaataggac actgtggaat gaaacagaac agagatcaaa aggtaatctg aagaatggca 120
<210> 480
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 480
tttgcttaaa atacaccatc ttgatggcct ctgcttctcc acttctatcc cctgggtgaa 60
gactcaattt ctcatccaca ctctgcagca agtcccagcc tggtcactac tgttattaca 120
<210> 481
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 481
tgagagtttt gaatgatcga tctcagaaat ggctgaaaac aaacttgccc ttttacatct 60
gtcccatgtg attcgatgcg tctcccattc cagggtgtgg gccggccaag gagagagggg 120
<210> 482
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 482
cgttccccag tgcccctaga gggcctgcca cggcttcccg atggcctgga tgtgctcctt 60
ggctttcaga cgcagcgccg cgatgctgct gttgcgcggg tccgcctcgt ccagcgggaa 120
<210> 483
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 483
cgcccgcccg ccctctgtcc gcgatggagg tgccggccgc gggtcgcgtt cctgccgagg 60
gcgccccgac ggcggctgtg gccgaggtgc gctgcccggg gcccgcgccg ctgcgcctgc 120
<210> 484
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 484
ctttccgcag ccatgctcaa acagagtgag aggagacggt cctggagcta caggccctgg 60
aacacgacgg agaatgaggg cagccaacac cgcaggagca tttgctccct gggtgcccgt 120
<210> 485
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 485
actcttattt caagcagaat gagtgctcct aaactcctct ctctgggctg tatcttcttc 60
cccttgctac tttttcagca ggcccgggct caattcccaa gacagtgtgc cactgttgag 120
<210> 486
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 486
tgaaatgctt tggcagtttt aagacatatc acagtctcta ccaagacata ctgacattaa 60
tgatgtccgt ggcgatggca attgccttga tggctcagtt tgaagagaca caatgttact 120
<210> 487
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 487
tgttcttata ggcaaagatg ctggaaggag atctggtttc aaagatgcta cgagctgttc 60
tgcagtctca taagaatgga gtagcattac cccggctcca aggagagtac agatccttga 120
<210> 488
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 488
aaatgtttgc tgcatgcgct cattgtctga atcattgttt caggttgctc cggttgctaa 60
gaagactatg aacaagtcag agaacctgct gtttgctggt tcatcattag catcacaagt 120
<210> 489
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 489
ctctgctggg cgcgcggtgg acggtctgaa agggagtgtt cgggtttcgc tggggcctcg 60
cggctccaga gcccagcatg gcttcctcgc gagcctcttc cacggtacag agcccgggat 120
<210> 490
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 490
agtggtggga ggccctcagg ccttggcctc tagagcatca cattgaactc agtgaccaga 60
aagtcaatgt agtcacggtc cttagtcttg aaggcctcgg cgataatgcg ggcggcctcc 120
<210> 491
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 491
ttctgagctg tgctagcaat acccttcaaa ggaagagcaa tggctgcagc agcagcttct 60
cacctgaacc tggatgccct ccgggaagtg ctagaatgcc ccatctgcat ggagtccttc 120
<210> 492
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 492
acccacgcca tcccttttct gccaaaagga gaaggggatg aatgaataca ggacttacgc 60
gtctgctgtg gcccagctgg cttccagatg gtgacatgag ccacccacag ccggagctgt 120
<210> 493
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 493
tggaccacgg ctcctccctt cttttctagg gcgtctgcct gagcccgctt ttctacaaga 60
tgtggggatt tttgaagcgc cctgtagtgg tgacggctga catcaacttg agccttgtgg 120
<210> 494
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 494
caatatgcaa gttaacactg attgaccatc attccttagc tgtgttcatg atgagtctca 60
ttgtagtcca tgatatgtag ctgtccaaca ctgtccgggg tcgggggaga cgggtgaggg 120
<210> 495
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 495
aaggcagtct tgaaatactg cattatccag ggacagggct tcagcagctg atctgtcaca 60
caccaggtgt cccacgtagg aatttcttaa accacaggta ggatgtagct gcagagagtc 120
<210> 496
<211> 120
<212> DNA
<213> Artificial Sequence
<400> 496
ggggaagccg tgggcggaag cggaagtgac gactgaagcg gggcggagac gcaagatggc 60
ggctgtggtg ctggcggcga cgcggttgct gcggggctcg ggttcttggg gctgttcgcg 120

Claims (11)

1.一种基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，所述应用包括以下步骤：
1)使用基因筛查芯片对致病基因进行筛查，确定出高度可疑的致病突变；
2)通过降落PCR(touchdown PCR)扩增方法，对致病基因的突变位点进行检测。
2.根据权利要求1所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述基因筛查芯片包括基因筛查芯片载体以及固定在所述载体上的寡核苷酸探针，所述寡核苷酸探针根据中国人特异的遗传性眼科疾病相关基因设计。
3.根据权利要求2所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述中国人特异的遗传性眼科疾病相关基因包括441个相关基因。
4.根据权利要求2所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述寡核苷酸探针的序列如SEQ ID NO.1～496所示。
5.根据权利要求1所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述步骤2)还包括提取待测样本的基因组DNA，设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增目标序列，对扩增产物进行酶切鉴定的方法进行分析，从而确定待测基因是否发生了突变。
6.根据权利要求1所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述步骤2)还包括提取待测样本的基因组DNA，设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增目标序列，对扩增产物通过sanger测序的方法进行分析，从而确定待测基因是否发生了突变。
7.根据权利要求1所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述步骤2)还包括提取待测样本的基因组DNA，设计特异性引物，利用降落PCR(touchdown PCR)的方法扩增目标序列，对扩增产物进行酶切鉴定和sanger测序的方法进行分析，从而确定待测基因是否发生了突变。
8.一种遗传性眼科疾病的基因筛查芯片，其特征在于：所述基因筛查芯片包括基因筛查芯片载体以及固定在所述载体上的寡核苷酸探针，所述寡核苷酸探针根据中国人特异的遗传性眼科疾病相关基因设计。
9.根据权利要求8所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述中国人特异的遗传性眼科疾病相关基因包括441个相关基因。
10.根据权利要求8所述的基因筛查芯片在制备遗传性眼科疾病诊断试剂盒中的应用，其特征在于：所述寡核苷酸探针的序列如SEQ ID NO.1～496所示。
11.一种诊断试剂盒，其特征在于，所述试剂盒包括权利要求8-10任一项所述的遗传性眼科疾病的基因筛查芯片。
CN201810256622.9A 2018-03-27 2018-03-27 一种筛查遗传性眼科疾病的基因芯片及检测方法 Pending CN108441554A (zh)

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