CN108432711A - A kind of breeding method improving the silkworm frequency of mutation - Google Patents
A kind of breeding method improving the silkworm frequency of mutation Download PDFInfo
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- CN108432711A CN108432711A CN201810632985.8A CN201810632985A CN108432711A CN 108432711 A CN108432711 A CN 108432711A CN 201810632985 A CN201810632985 A CN 201810632985A CN 108432711 A CN108432711 A CN 108432711A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 134
- 230000035772 mutation Effects 0.000 title claims abstract description 33
- 238000009395 breeding Methods 0.000 title claims abstract description 32
- 240000000249 Morus alba Species 0.000 claims abstract description 109
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 109
- 239000007788 liquid Substances 0.000 claims abstract description 57
- 238000012545 processing Methods 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000013011 mating Effects 0.000 claims abstract description 10
- 230000017448 oviposition Effects 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 241000723353 Chrysanthemum Species 0.000 claims description 53
- 235000007516 Chrysanthemum Nutrition 0.000 claims description 53
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
- 239000008367 deionised water Substances 0.000 claims description 37
- 229910021641 deionized water Inorganic materials 0.000 claims description 37
- 239000007864 aqueous solution Substances 0.000 claims description 33
- 241000196324 Embryophyta Species 0.000 claims description 22
- -1 compound amino acid Chemical class 0.000 claims description 22
- 239000011780 sodium chloride Substances 0.000 claims description 22
- 241000251468 Actinopterygii Species 0.000 claims description 18
- 239000002202 Polyethylene glycol Substances 0.000 claims description 18
- 229920001223 polyethylene glycol Polymers 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 18
- 238000000605 extraction Methods 0.000 claims description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 10
- 230000012447 hatching Effects 0.000 claims description 10
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 9
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 9
- 230000001476 alcoholic effect Effects 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 238000011534 incubation Methods 0.000 claims description 9
- 239000004571 lime Substances 0.000 claims description 9
- 235000019991 rice wine Nutrition 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000002242 deionisation method Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 208000027418 Wounds and injury Diseases 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 4
- 208000014674 injury Diseases 0.000 abstract description 4
- 231100000219 mutagenic Toxicity 0.000 abstract description 3
- 230000003505 mutagenic effect Effects 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 10
- 150000001413 amino acids Chemical class 0.000 description 7
- 231100000350 mutagenesis Toxicity 0.000 description 6
- 238000002703 mutagenesis Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Fodder In General (AREA)
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Abstract
The invention discloses a kind of breeding methods improving the silkworm frequency of mutation, include the following steps：Silkworm hatches, larva is raised to newly-hatched silkworm and was fed to for 3 ages, then it is raised again to silkworm stopping feeding with self-control feed after being raised to 4 ages with liquid processing mulberry leaf and is spun, cocoond and sprouted wings, it again will treated emerging adult and untreated emerging adult mating, oviposition, it is next-generation up to raising silkworm, and the selection of mutated individual is carried out to the raising silkworm next generation of gained.Silkworm breeding is carried out using the present invention, the silkworm frequency of mutation can be effectively improved, mulberry leaf and self-control feed are handled by feeding liquid, reduces injury of the mutagenic processes to silkworm, while cost is relatively low, has a vast market foreground.
Description
Technical field
The present invention relates to mutation breeding technologies field, specifically a kind of breeding method for improving the silkworm frequency of mutation.
Background technology
Silkworm is called silkworm, is a kind of Important Economic insect to be cocoond as the spinning of foodstuff using mulberry leaf, particularly belongs to silkworm
The silkworm moth category Silkworm of moth section.The main purpose object of planting mulberry and breeding silkworm is silk cocoon and silk, and silkworm all one's life is by ovum, larva, pupa, adult
Entirely different stage of development, the different parts and its metabolin of each stage of development in four forms and on physiological function
There is extensive medical value.
Mutagenesis is morphed by artificial measure induction of genetic gene, then according to need in the mutational variety of generation
Seek the method for selecting improved seeds.Common silkworm method of mutagenesis is to carry out mutagenesis using physical factor and chemical factor.Wherein,
Physical factor is to handle mutant materials using various rays, microwave or laser etc., larger to the injury of silkworm, meanwhile, mutagenesis
It needs instrument to be used more, considerably increases cost.Therefore, a kind of breeding method progress improving the silkworm frequency of mutation is designed
Silkworm mutagenesis, the problem of becoming urgent need to resolve.
Invention content
The purpose of the present invention is to provide a kind of breeding methods improving the silkworm frequency of mutation, to solve above-mentioned background technology
The problem of middle proposition.
To achieve the above object, the present invention provides the following technical solutions：
A kind of breeding method improving the silkworm frequency of mutation, which is characterized in that include the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 2-4min, then drying is put into temperature
Case is hatched, and larvae is obtained；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, feeding to ant
Silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
The liquid processing mulberry leaf are the mulberry leaf handled by following methods：The new fresh mulberry leaf of rigid picking back is clear
Dewaxing treatment is carried out after wash clean, then mulberry leaf are impregnated using Aqueous Solutions of Polyethylene Glycol, with by disappearing after the completion of impregnating
The dry gauze of poison dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains the medicine
Liquid handles mulberry leaf；
The self-control feed includes below according to raw material：0.001-0.05 parts of chrysanthemum cinesariaefolium extract determines worm
0.005-0.05 parts of precititation solution, 4-12 parts of plant type compound amino acid, 0.01-0.09 parts of sodium chloride, is gone 82-98 parts of mulberry leaf
5-15 parts of ionized water.
As a further solution of the present invention：In step 1), the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity
69%.
As further scheme of the invention：In step 2), the temperature of the feeding is 22 DEG C.
As further scheme of the invention：In step 3), the self-control feed includes below according to raw material：
0.01-0.04 parts of chrysanthemum cinesariaefolium extract, 0.02-0.04 parts of UC 62644 aqueous solution, 85-92 parts of mulberry leaf, the compound ammonia of plant type
7-10 parts of base acid, 0.04-0.07 parts of sodium chloride, 8-11 parts of deionized water.
As further scheme of the invention：In step 3), the self-control feed includes below according to raw material：
0.02 part of chrysanthemum cinesariaefolium extract, 0.03 part of UC 62644 aqueous solution, 90 parts of mulberry leaf, 9 parts of plant type compound amino acid, sodium chloride
0.05 part, 10 parts of deionized water.
As further scheme of the invention：The preparation method of the self-control feed is to weigh deionization according to parts by weight
Water sequentially adds chrysanthemum cinesariaefolium extract, UC 62644 aqueous solution and sodium chloride and carries out ultrasonic disperse processing, then input stirring
It in machine and mulberry leaf and plant type compound amino acid is added is uniformly mixed, cross 200 mesh and screen out liquid and obtain the self-control feed.
As further scheme of the invention：The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid.
As further scheme of the invention：The preparation method of the chrysanthemum cinesariaefolium extract is to weigh white flower deinsectization
Chrysanthemum 8g, is cleaned 3-5 times with deionized water, then deionized water 8000g is added in drying, is carried out using steam pressure cooker equipment high
The chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in warm high pressure extraction, extracting solution.
Application of the breeding method of the raising silkworm frequency of mutation in silkworm breeding.
Compared with prior art, the beneficial effects of the invention are as follows：
Silkworm breeding is carried out using the present invention, the silkworm frequency of mutation can be effectively improved, mulberry leaf are handled by feeding liquid
Mutagenesis is carried out with self-control feed, mutagenic processes are relatively low to the injury of silkworm, while cost is relatively low, has a vast market foreground.
Specific implementation mode
Technical scheme of the present invention is described in more detail With reference to embodiment.
Embodiment 1
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.001 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.005 part of liquid, 82 parts of mulberry leaf, 4 parts of plant type compound amino acid, 0.01 part of sodium chloride, 5 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 2
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.05 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.05 part of liquid, 98 parts of mulberry leaf, 12 parts of plant type compound amino acid, 0.09 part of sodium chloride, 15 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 3
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.025 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.03 part of liquid, 90 parts of mulberry leaf, 8 parts of plant type compound amino acid, 0.05 part of sodium chloride, 10 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 4
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.01 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.02 part of liquid, 85 parts of mulberry leaf, 7 parts of plant type compound amino acid, 0.04 part of sodium chloride, 8 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 5
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.04 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.04 part of liquid, 92 parts of mulberry leaf, 10 parts of plant type compound amino acid, 0.07 part of sodium chloride, 11 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 6
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.025 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.03 part of liquid, 88.5 parts of mulberry leaf, 8.5 parts of plant type compound amino acid, 0.055 part of sodium chloride, 9.5 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Embodiment 7
A kind of breeding method improving the silkworm frequency of mutation, includes the following steps：
1) silkworm hatches：Silkworm egg is placed in the pure glutinous rice wine that alcoholic strength is 25 ° and impregnates 3min, then drying is put into incubator
To be hatched, the condition of the hatching is 26 DEG C of incubation temperature, hatches humidity 69%, obtains larvae,；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, the feeding
Temperature be 22 DEG C, feeding to newly-hatched silkworm was fed to for 3 ages, must raise larva；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina point
For processing group larva and untreated fish group larva, untreated fish group larva using mulberry leaf continue feeding to silkworm stop feeding being spun,
It cocoons and sprouts wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is carried out with liquid processing mulberry leaf
Feeding stops feeding to silkworm and is spun, cocoond and sprouted wings to being raised again with self-control feed after 4 ages, obtain that treated and sprout wings at
Worm；Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation and right to get raising silkworm
The raising silkworm next generation of gained carries out the selection of mutated individual；
Wherein, the liquid processing mulberry leaf are the mulberry leaf handled by following methods：Rigid picking is returned fresh
Mulberry leaf carry out dewaxing treatment after cleaning up, and are then impregnated to mulberry leaf using Aqueous Solutions of Polyethylene Glycol, are used after the completion of impregnating
Dry gauze by disinfection dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains institute
The liquid processing mulberry leaf stated；
The self-control feed includes below according to raw material：0.02 part of chrysanthemum cinesariaefolium extract, UC 62644 are water-soluble
0.03 part of liquid, 90 parts of mulberry leaf, 9 parts of plant type compound amino acid, 0.05 part of sodium chloride, 10 parts of deionized water；
The preparation method of the self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extraction
Object, UC 62644 aqueous solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and are added mulberry leaf and plant type is multiple
It closes amino acid to be uniformly mixed, 200 mesh screen out liquid and obtain the self-control feed excessively；
The UC 62644 aqueous solution is that 5% UC 62644 dilutes 5000 times of liquid；The preparation side of the chrysanthemum cinesariaefolium extract
Method is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 5 times with deionized water, is dried, and deionized water 8000g is then added, using vapour pressure
Power pot equipment carries out high temperature and pressure extraction, and the chrysanthemum cinesariaefolium extract is obtained by filtration through 1400 mesh again in extracting solution.
Comparative example 1
Compared with Example 7, chrysanthemum cinesariaefolium extract is free of, other are same as Example 7.
Comparative example 2
Compared with Example 7, UC 62644 aqueous solution is free of, other are same as Example 7.
Comparative example 3
Compared with Example 7, chrysanthemum cinesariaefolium extract and UC 62644 aqueous solution are free of, other are same as Example 7.
Experiment
Breeding is carried out using the method described in embodiment 7 and comparative example 1-3 and obtains the corresponding raising silkworm next generation,
2000 silkworm eggs are randomly selected in the raising silkworm next generation that embodiment 7 obtains and carry out cultivation raising, in the feeding that comparative example 1 obtains
It supports the family and randomly selects 2000 silkworm eggs in the silkworm next generation and carry out cultivation raising, in the raising silkworm next generation that comparative example 2 obtains
It randomly selects 2000 silkworm eggs and carries out cultivation raising, 2000 are randomly selected in the raising silkworm next generation that comparative example 3 obtains
Silkworm egg carries out cultivation raising, mutation rate analysis and correlated traits investigation is carried out in feeding process, the results are shown in Table 1.
1 test result table of table
Pass through addition chrysanthemum cinesariaefolium extraction from can be seen that the present invention in the data comparison of embodiment 7 and comparative example 1
Object can effectively improve the silkworm frequency of mutation；As can be seen that the present invention passes through from data comparison of the embodiment 7 with comparative example 2
UC 62644 aqueous solution is added, the silkworm frequency of mutation can be effectively improved；In addition, from the data comparison of embodiment 7 and comparative example 1-3
In as can be seen that the present invention by the identical cooperation with UC 62644 aqueous solution of chrysanthemum cinesariaefolium extract, play synergistic function,
The silkworm frequency of mutation can be further effectively improved, by the morphological observation of the raising individual to embodiment 7, finds only speckle
The individual to morph has 52, and mutation rate reaches 2.6%.
As can be seen that carrying out silkworm breeding using the present invention from result above, the silkworm frequency of mutation can be effectively improved,
Mulberry leaf and self-control feed are handled by feeding liquid, reduce injury of the mutagenic processes to silkworm, while cost is relatively low, are had wide
Wealthy market prospects.
The better embodiment of the present invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party
Formula, one skilled in the relevant art within the scope of knowledge, can also be without departing from the purpose of the present invention
Various changes can be made.
Claims (9)
1. a kind of breeding method improving the silkworm frequency of mutation, which is characterized in that include the following steps：
1) silkworm hatches：It is to impregnate 2-4min in 25 ° of pure glutinous rice wine that silkworm egg, which is placed in alcoholic strength, then drying be put into incubator into
Row hatching, obtains larvae；
2) larva is raised：The larvae obtained in step 1) is put into container and is raised with mulberry leaf, feeding to newly-hatched silkworm is fed
To 3 ages, larva must be raised；
3) feed is raised：The feeding larva obtained in step 2) is subjected to disinfection of silkworm body with lime, then stochastic averagina is divided into place
Reason group larva and untreated fish group larva, untreated fish group larva continue feeding to silkworm stopping feeding using mulberry leaf and are spun, cocoon
And sprout wings, obtain untreated emerging adult；Meanwhile processing group larva is put into rearing-box and is raised with liquid processing mulberry leaf
It is raised again with self-control feed after to 4 ages and stops feeding to silkworm and spun, cocoond and sprouted wings, the emerging adult that obtains that treated；
Then will treated emerging adult and untreated emerging adult mating, oviposition is next-generation to get raising silkworm, and to gained
Raising the silkworm next generation carry out mutated individual selection；
The liquid processing mulberry leaf are the mulberry leaf handled by following methods：The new fresh mulberry leaf cleaning of rigid picking back is dry
Dewaxing treatment is carried out after net, then mulberry leaf are impregnated using Aqueous Solutions of Polyethylene Glycol, with by disinfection after the completion of impregnating
Dry gauze dries the polyethylene glycol for remaining in mulberry leaf surface, is then cut into the strip of 3mm × 20mm, obtains at the liquid
Manage mulberry leaf；
The self-control feed includes below according to raw material：0.001-0.05 parts of chrysanthemum cinesariaefolium extract, UC 62644 water
0.005-0.05 parts of solution, 82-98 parts of mulberry leaf, 4-12 parts of plant type compound amino acid, 0.01-0.09 parts of sodium chloride, deionization
5-15 parts of water.
2. the breeding method according to claim 1 for improving the silkworm frequency of mutation, which is characterized in that described in step 1)
The condition of hatching is 26 DEG C of incubation temperature, hatches humidity 69%.
3. the breeding method according to claim 2 for improving the silkworm frequency of mutation, which is characterized in that described in step 2)
The temperature of feeding is 22 DEG C.
4. the breeding method according to claim 1 for improving the silkworm frequency of mutation, which is characterized in that described in step 3)
It includes below according to raw material to make feed by oneself：0.01-0.04 parts of chrysanthemum cinesariaefolium extract, UC 62644 aqueous solution 0.02-
0.04 part, 85-92 parts of mulberry leaf, 7-10 parts of plant type compound amino acid, 0.04-0.07 parts of sodium chloride, 8-11 parts of deionized water.
5. the breeding method according to claim 4 for improving the silkworm frequency of mutation, which is characterized in that described in step 3)
It includes below according to raw material to make feed by oneself：0.02 part of chrysanthemum cinesariaefolium extract, 0.03 part of UC 62644 aqueous solution, mulberry leaf
90 parts, 9 parts of plant type compound amino acid, 0.05 part of sodium chloride, 10 parts of deionized water.
6. the breeding method according to claim 5 for improving the silkworm frequency of mutation, which is characterized in that described in step 3)
The preparation method of self-control feed is to weigh deionized water according to parts by weight, sequentially adds chrysanthemum cinesariaefolium extract, UC 62644 water
Solution and sodium chloride carry out ultrasonic disperse processing, then put into blender and be added mulberry leaf and the mixing of plant type compound amino acid
Uniformly, 200 mesh screen out liquid and obtain the self-control feed excessively.
7. the breeding method according to claim 6 for improving the silkworm frequency of mutation, which is characterized in that the UC 62644 is water-soluble
Liquid is that 5% UC 62644 dilutes 5000 times of liquid.
8. the breeding method according to claim 6 for improving the silkworm frequency of mutation, which is characterized in that the chrysanthemum cinesariaefolium
The preparation method of extract is to weigh chrysanthemum cinesariaefolium 8g, is cleaned 3-5 times with deionized water, dries, deionized water is then added
8000g carries out high temperature and pressure extraction using steam pressure cooker equipment, and the white flower deinsectization is obtained by filtration through 1400 mesh again in extracting solution
Chrysanthemum extract.
9. a kind of breeding method the answering in silkworm breeding of raising silkworm frequency of mutation as described in claim 1-8 is any
With.
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| CN103621475A (en) * | 2013-11-06 | 2014-03-12 | 浙江大学 | Breeding method for improving mutation frequency of silkworms |
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| CN1418543A (en) * | 2002-09-05 | 2003-05-21 | 浙江省农业科学院 | Method for utilization of biological reaction device specially used for variety development of silkworm |
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