CN108424472A - Ganoderma active polysaccharide - Google Patents
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- CN108424472A CN108424472A CN201810408292.0A CN201810408292A CN108424472A CN 108424472 A CN108424472 A CN 108424472A CN 201810408292 A CN201810408292 A CN 201810408292A CN 108424472 A CN108424472 A CN 108424472A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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Abstract
The invention discloses ganoderma active polysaccharide, preparation method is：Glossy ganoderma powder after crushing is extracted using Primary organic solvent under cryogenic, it is filtered after extraction, collect filtrate, then the deionized water containing sorbierite and L dimethyl tartrates is added in filter residue to stir evenly, cellulase and compound protease is added in substep, and microwave radiation technology digests, controlled enzymatic hydrolysis temperature in enzymolysis process, time and pH, after enzyme deactivation, obtain enzymolysis liquid, enzymolysis liquid is concentrated under reduced pressure later, obtain ganoderma lucidum crude polysaccharide concentrate, composite decoloring agent is eventually adding to be handled, it is centrifuged after filtering, supernatant spray drying is obtained into ganoderma active polysaccharide finished product.Ganoderma active polysaccharide purity of the present invention is high, color and luster is good, active skull cap components are high, best in quality, has notable economic value, preparation method is easy quickly, recovery rate is high, low energy consumption, pollution-free, is suitable for industrialized production.
Description
Technical field
The present invention relates to Polyose extraction fields, more particularly, to ganoderma active polysaccharide.
Technical background
Ganoderma lucidum is the treasure in China's pools of traditional Chinese medicine, has the effect of preventing and curing diseases, promoting longevity, is known as " celestial grass "
Reputation.The Eastern Han Dynasty《Sheng Nong's herbal classic》, the Ming Dynasty famous medical scholar's Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》, all to ganoderma lucidum the effect of
It is documented in detail.Nowadays, ganoderma lucidum has been used as drug formally to be recorded by National Pharmacopeia, while it is the new resources of state approval again
Food has no toxic side effect, can be with medicine-food two-purpose.
Since latter stage age in this century, since Ganoderma Lucidum fructification succeeds, then deep layer hair is developed again
The development and application of the technology of ferment culture ganoderma lucidum mycelium and zymotic fluid, ganoderma lucidum are increasingly extensive.To the chemical composition of ganoderma lucidum class fungi,
Pharmacological action and mechanism of action and clinical application have carried out a large amount of research, and achieve major progress.These research shows that
Ganoderma lucidum polysaccharide is one of most effective active constituent of ganoderma lucidum, has extensive pharmacological activity, can improve immunity of organisms, is improved
Body's hypoxia tolerance eliminates free radical, and tumour, radioresistance is inhibited to improve liver, marrow, blood synthetic DNA, RNA and albumen
The ability of matter extends service life etc., therefore, is especially paid attention to by medical sci-tech worker.
It is varied for the extracting method of active polysaccharide in ganoderma lucidum at present, such as traditional water extraction method, organic solvent
The extracting methods such as extraction method and its existing more common Soxhlet, ultrasonic wave, microwave, enzymolysis, but it is single and each due to method
From the limitation of condition, there are the recovery rate of ganoderma lucidum polysaccharide and purity be not high, extraction process is complicated, and loss of effective components is more etc. asks
Topic.
The prior art such as Authorization Notice No. is the Chinese invention patent of 105017441 B of CN, discloses a kind of ganoderma lucidum polysaccharide
Extracting method, include the following steps：1）Ganoderma lucidum ethyl alcohol is extracted, takes filter residue spare；2）Filter residue water is carried, it is standby to collect filtrate
With；3）By filtrate concentrated extract, ethanol precipitation centrifuges to obtain precipitation；4）Precipitation successively absolute ethyl alcohol, acetone is washed respectively, it will
Precipitation distillation water dissolution after washing, crosses the hollow-fibre membrane that molecular cut off is 50,000, collects concentrate, then will concentration
Liquid crosses the hollow-fibre membrane that molecular cut off is 80,000, collects permeate centrifugation, and filtering takes filtrate spare；5）It is added and lives toward filtrate
Property carbon decoloring, filtering, take filtrate to be spray-dried, as ganoderma lucidum polysaccharide.The ganoderma lucidum polysaccharide purity of this method extraction is high, and color and luster is clean
In vain, and antitumor activity is strong.But the extracting method recovery rate is not high, and extracted many times are needed, the operating time is longer.
Invention content
The purpose of the present invention is to provide a kind of purity height, color and luster is good, active skull cap components are high, and ganoderma lucidum best in quality lives
Property polysaccharide, preparation method is easy quickly, recovery rate is high, low energy consumption, pollution-free, is suitable for industrialized production.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：
Ganoderma active polysaccharide, preparation method includes the extraction of low temperature organic solvent, microwave radiation technology digests and enzymolysis liquid purification process,
It is as follows：
Low temperature organic solvent extracts：The ganoderma lucidum dried is selected, 60-80 mesh sieve is smashed it through, obtains glossy ganoderma powder, water is added in glossy ganoderma powder
And stir evenly, the weight ratio of glossy ganoderma powder and water is 1:8-10 impregnates 5-7h at 50-60 DEG C, is filtered after immersion, receives
Collect filtrate, filter residue is extracted into 2-3h using organic solvent, is filtered after extraction, collects filtrate, filter residue is spare, active polysaccharide
Drug activity is complete dependent on its space triple-helix structure, and this triple-helix structure is maintained by hydrogen bond, is held at high temperature
Binding force between destructible hydrogen bond, low temperature immersion can reduce to being destroyed caused by space structure and activity, can preferably retain
The natural activity of ganoderma lucidum polysaccharide is conducive to the quality for improving ganoderma lucidum polysaccharide；
Microwave radiation technology digests：The deionization containing sorbierite Yu L-TARTARIC ACID dimethyl ester of 6-8 times of filter residue weight is added in filter residue
Water stirs evenly, and is firstly added filter residue weight 0.5-0.9% cellulases, 40-45 DEG C of controlled enzymatic hydrolysis temperature, pH value 4.3-5.0,
And place it in Microwave Extraction Equipment and extract, the power of microwave is 400-600W, frequency 800-1000MHZ, digests 20-
30min, after add the compound protease of filter residue weight 0.2-0.6%, 45-50 DEG C of controlled enzymatic hydrolysis temperature, pH value 6.8-
7.5, it is equally placed in Microwave Extraction Equipment and extracts, the power of microwave is 400-600W, frequency 800-1000MHZ, digests 20-
30min, after enzymolysis at 100-105 DEG C enzyme deactivation 5-10min, obtain enzymolysis liquid, microwave radiation technology enzyme hydrolysis can be destroyed effectively carefully
Cell wall accelerates the speed that solvent enters and carry solute dissolution cell, is conducive to the release of intracellular polyse, substantially increases ganoderma lucidum
The recovery rate of polysaccharide；
Enzymolysis liquid purification process：Obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, decompression low temperature is concentrated into enzymolysis liquid extremely
The 1/3 of original volume obtains ganoderma lucidum crude polysaccharide concentrate, and the composite decoloring agent of ganoderma lucidum crude polysaccharide concentrate 3-5%, stirring is then added
It filters after 30-40min, and is centrifuged in the case where rotating speed is 4000-4500rpm, obtain supernatant, supernatant spray drying is obtained
Ganoderma active polysaccharide finished product, prepared ganoderma active polysaccharide purity is high, color and luster is good, active skull cap components are high, can be answered extensively
For fields such as feed addition, health care of food, cosmetics.
Preferably, organic solvent is the ethanol solution of a concentration of 80-95%, polysaccharide is the aldehydes or ketones of polyhydroxy, is dissolved in
Water, ethanol solution can destroy the hydrogen bond in polysaccharide solution, to reduce the solubility of polysaccharide in water, make polysaccharide to precipitate
Form is precipitated, and achievees the purpose that tentatively to extract.
Preferably, in deionized water the sorbierite containing 0.2-0.4% and 0.03-0.05% L-TARTARIC ACID dimethyl ester,
Ganoderma lucidum filter residue is handled using the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester, on the one hand can be made with swollen ganoderma lucidum slag
Cellulase and compound protease can be contacted rapidly and efficiently with fat, the cellulose in ganoderma lucidum, and then accelerate enzymolysis rate, be made
Intracellular polyse is more discharged, and is conducive to improve polysaccharide extract rate；Another aspect sorbierite is taken with L-TARTARIC ACID dimethyl ester
With use, synergistic effect is played, intercellular layer structure in cell wall can be effectively destroyed, accelerate the rupture of cell wall, make intracellular
Effective polysaccharide is released diffusion, further increases extraction efficiency.
Preferably, it is 1 that compound protease, which is weight proportion,:The alkali protease of 2-4 and the mixture of papain.
Preferably, the material composition and its parts by weight of composite decoloring agent are：9-15 parts of atlapulgite, beta-cyclodextrin 5-8
Part, 1-2 parts of citicoline, atlapulgite contains a large amount of micropores, has huge specific surface area, to the adsorption capacity pole of pigment
By force, some malodor components, polycyclic aromatic hydrocarbon and remains of pesticide can also be removed, citicoline and beta-cyclodextrin play synergistic effect can
More to be adsorbed in Activation Activity carclazyte a little, decolorizing efficiency can be further increased, coloration and stink can be effectively removed, made
It is good to obtain ganoderma active polysaccharide purity height, the color and luster finally prepared.
Compared with the prior art, the advantages of the present invention are as follows：1）Ganoderma active polysaccharide purity of the present invention is high, color and luster is good, day
Right active constituent is high, best in quality, has notable economic value；2）Ganoderma active polysaccharide preparation method of the present invention is easy quickly,
Recovery rate is high, low energy consumption, pollution-free, is suitable for industrialized production.
Specific implementation mode
The present invention program is described further below by embodiment：
Embodiment 1：
Ganoderma active polysaccharide, preparation method includes the extraction of low temperature organic solvent, microwave radiation technology digests and enzymolysis liquid purification process,
It is as follows：
1）Low temperature organic solvent extracts：The ganoderma lucidum dried is selected, 60 mesh sieve is smashed it through, obtains glossy ganoderma powder, water is added in glossy ganoderma powder
And stir evenly, the weight ratio of glossy ganoderma powder and water is 1:8,5h is impregnated at 50 DEG C, is filtered after immersion, and filtrate is collected,
Filter residue is extracted into 2h using organic solvent, is filtered after extraction, collects filtrate, filter residue is spare, the drug activity of active polysaccharide
Dependent on the complete of its space triple-helix structure, and this triple-helix structure is maintained by hydrogen bond, is easily destroyed hydrogen at high temperature
Binding force between key, low temperature immersion can be reduced to being destroyed caused by space structure and activity, can preferably retain ganoderma lucidum polysaccharide
Natural activity, be conducive to improve ganoderma lucidum polysaccharide quality；
2）Microwave radiation technology digests：The deionization containing sorbierite Yu L-TARTARIC ACID dimethyl ester of 6 times of filter residue weight is added in filter residue
Water stirs evenly, and is firstly added 0.5% cellulase of filter residue weight, 40 DEG C, pH value 4.3-5.0 of controlled enzymatic hydrolysis temperature, and by its
Be placed in Microwave Extraction Equipment and extract, the power of microwave is 400W, frequency 1000MHZ, digests 20min, after add
The compound protease of filter residue weight 0.3%, controlled enzymatic hydrolysis temperature 45 C, pH value 6.8-7.5 are equally placed in Microwave Extraction Equipment
Extraction, the power of microwave are 400W, frequency 1000MHZ, digest 20min, after enzymolysis at 100 DEG C enzyme deactivation 5min, must digest
Liquid, microwave radiation technology enzyme hydrolysis can effectively destroy cell wall, accelerate the speed that solvent enters and carry solute dissolution cell, have
Conducive to the release of intracellular polyse, the recovery rate of ganoderma lucidum polysaccharide is substantially increased；
3）Enzymolysis liquid purification process：Obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, decompression low temperature is concentrated into enzymolysis liquid
To the 1/3 of original volume, ganoderma lucidum crude polysaccharide concentrate is obtained, the composite decoloring agent of ganoderma lucidum crude polysaccharide concentrate 3%, stirring is then added
It filters after 30min, and is centrifuged in the case where rotating speed is 4000rpm, obtain supernatant, supernatant spray drying is obtained into ganoderma active
Polysaccharide finished product, prepared ganoderma active polysaccharide purity is high, color and luster is good, active skull cap components are high, can be widely used in feed
The fields such as addition, health care of food, cosmetics.
The ethanol solution that above-mentioned organic solvent is a concentration of 80%, polysaccharide is the aldehydes or ketones of polyhydroxy, water-soluble, ethyl alcohol
Solution can destroy the hydrogen bond in polysaccharide solution, to reduce the solubility of polysaccharide in water, polysaccharide be made to be analysed in the form of precipitating
Go out, achievees the purpose that tentatively to extract.
L-TARTARIC ACID dimethyl ester containing 0.2% sorbierite and 0.03% in above-mentioned deionized water, using containing sorbierite
Ganoderma lucidum filter residue is handled with the deionized water of L-TARTARIC ACID dimethyl ester, on the one hand can make cellulase and compound with swollen ganoderma lucidum slag
Protease can be contacted rapidly and efficiently with fat, the cellulose in ganoderma lucidum, and then accelerate enzymolysis rate, and intracellular polyse is made to obtain more
More release is conducive to improve polysaccharide extract rate；Another aspect sorbierite is used with the collocation of L-TARTARIC ACID dimethyl ester, plays collaboration
Effect, can effectively destroy intercellular layer structure in cell wall, accelerate the rupture of cell wall, intracellular effective polysaccharide is made to be released
Diffusion, further increases extraction efficiency.
Above-mentioned compound protease is that weight proportion is 1:2 alkali protease and the mixture of papain.
The material composition and its parts by weight of above-mentioned composite decoloring agent be：10 parts of atlapulgite, 6 parts of beta-cyclodextrin, born of the same parents' phosphorus courage
2 parts of alkali, atlapulgite contains a large amount of micropores, has huge specific surface area, extremely strong to the adsorption capacity of pigment, can also remove
Some malodor components, polycyclic aromatic hydrocarbon and remains of pesticide, citicoline and beta-cyclodextrin play synergistic effect can be white with Activation Activity
It is more adsorbed in soil a little, decolorizing efficiency can be further increased, coloration and stink can be effectively removed so that finally prepare
Ganoderma active polysaccharide purity is high, color and luster is good.
The recovery rate of ganoderma active polysaccharide manufactured in the present embodiment is：7.86%.
Embodiment 2：
Ganoderma active polysaccharide, preparation method includes the extraction of low temperature organic solvent, microwave radiation technology digests and enzymolysis liquid purification process,
It is as follows：
1）Low temperature organic solvent extracts：The ganoderma lucidum dried is selected, 80 mesh sieve is smashed it through, obtains glossy ganoderma powder, water is added in glossy ganoderma powder
And stir evenly, the weight ratio of glossy ganoderma powder and water is 1:9,5h is impregnated at 60 DEG C, is filtered after immersion, and filtrate is collected,
Filter residue is extracted into 3h using organic solvent, is filtered after extraction, collects filtrate, filter residue is spare；
2）Microwave radiation technology digests：The deionization containing sorbierite Yu L-TARTARIC ACID dimethyl ester of 6 times of filter residue weight is added in filter residue
Water stirs evenly, and is firstly added 0.7% cellulase of filter residue weight, controlled enzymatic hydrolysis temperature 45 C, pH value 4.3-5.0, and by its
Be placed in Microwave Extraction Equipment and extract, the power of microwave is 600W, frequency 800MHZ, digests 30min, after add filter
The compound protease of slag weight 0.4%, controlled enzymatic hydrolysis temperature 45 C, pH value 6.8-7.5 are equally placed in Microwave Extraction Equipment and carry
Take, the power of microwave is 600W, frequency 800MHZ, digests 30min, after enzymolysis at 100 DEG C enzyme deactivation 5min, obtain enzymolysis liquid；
3）Enzymolysis liquid purification process：Obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, decompression low temperature is concentrated into enzymolysis liquid
To the 1/3 of original volume, ganoderma lucidum crude polysaccharide concentrate is obtained, the composite decoloring agent of ganoderma lucidum crude polysaccharide concentrate 5%, stirring is then added
It filters after 30min, and is centrifuged in the case where rotating speed is 4000rpm, obtain supernatant, supernatant spray drying is obtained into ganoderma active
Polysaccharide finished product.
The ethanol solution that above-mentioned organic solvent is a concentration of 95%.
L-TARTARIC ACID dimethyl ester containing 0.4% sorbierite and 0.04% in above-mentioned deionized water.
Above-mentioned compound protease is that weight proportion is 1:4 alkali protease and the mixture of papain.
The material composition and its parts by weight of above-mentioned composite decoloring agent be：15 parts of atlapulgite, 6 parts of beta-cyclodextrin, born of the same parents' phosphorus courage
2 parts of alkali.
The recovery rate of ganoderma active polysaccharide manufactured in the present embodiment is：8.02%.
Embodiment 3：
Ganoderma active polysaccharide, preparation method includes the extraction of low temperature organic solvent, microwave radiation technology digests and enzymolysis liquid purification process,
It is as follows：
1）Low temperature organic solvent extracts：The ganoderma lucidum dried is selected, 80 mesh sieve is smashed it through, obtains glossy ganoderma powder, water is added in glossy ganoderma powder
And stir evenly, the weight ratio of glossy ganoderma powder and water is 1:9,6h is impregnated at 60 DEG C, is filtered after immersion, and filtrate is collected,
Filter residue is extracted into 2h using organic solvent, is filtered after extraction, collects filtrate, filter residue is spare；
2）Microwave radiation technology digests：The deionization containing sorbierite Yu L-TARTARIC ACID dimethyl ester of 8 times of filter residue weight is added in filter residue
Water stirs evenly, and is firstly added 0.6% cellulase of filter residue weight, controlled enzymatic hydrolysis temperature 45 C, pH value 4.3-5.0, and by its
Be placed in Microwave Extraction Equipment and extract, the power of microwave is 400W, frequency 800MHZ, digests 20min, after add filter
The compound protease of slag weight 0.5%, controlled enzymatic hydrolysis temperature 50 C, pH value 6.8-7.5 are equally placed in Microwave Extraction Equipment and carry
Take, the power of microwave is 400W, frequency 800MHZ, digests 20min, after enzymolysis at 100 DEG C enzyme deactivation 10min, obtain enzymolysis liquid；
3）Enzymolysis liquid purification process：Obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, decompression low temperature is concentrated into enzymolysis liquid
To the 1/3 of original volume, ganoderma lucidum crude polysaccharide concentrate is obtained, the composite decoloring agent of ganoderma lucidum crude polysaccharide concentrate 4%, stirring is then added
It filters after 30min, and is centrifuged in the case where rotating speed is 4500rpm, obtain supernatant, supernatant spray drying is obtained into ganoderma active
Polysaccharide finished product.
The ethanol solution that above-mentioned organic solvent is a concentration of 90%.
L-TARTARIC ACID dimethyl ester containing 0.2% sorbierite and 0.05% in above-mentioned deionized water.
Above-mentioned compound protease is that weight proportion is 1:3 alkali protease and the mixture of papain.
The material composition and its parts by weight of above-mentioned composite decoloring agent be：15 parts of atlapulgite, 7 parts of beta-cyclodextrin, born of the same parents' phosphorus courage
1 part of alkali.
The recovery rate of ganoderma active polysaccharide manufactured in the present embodiment is：7.76%.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. at the preparation method of ganoderma active polysaccharide, including the extraction of low temperature organic solvent, microwave radiation technology enzymolysis and enzymolysis liquid purifying
Reason, it is characterised in that：The microwave radiation technology digests step：It is added containing sorbierite and L-TARTARIC ACID dimethyl ester in ganoderma lucidum filter residue
Deionized water stir evenly, then cellulase and compound protease is added in substep, and assisted microwave synthesis is digested, and controls enzyme
Solution temperature, time and pH, enzyme deactivation obtain enzymolysis liquid.
2. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：Contain in the deionized water
The sorbierite of 0.2-0.4% and the L-TARTARIC ACID dimethyl ester of 0.03-0.05%.
3. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The addition of the cellulase
Amount is the 0.5-0.9% of ganoderma lucidum filter residue weight, it is described using cellulase degradation when hydrolysis temperature be 40-45 DEG C, the time is
20-30min, pH 4.3-5.0.
4. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The compound protease is attached most importance to
Amount proportioning is 1:The alkali protease of 2-4 and the mixture of papain.
5. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The compound protease adds
Dosage is the 0.2-0.6% of ganoderma lucidum filter residue weight, and hydrolysis temperature when compound protease enzymolysis is 45-50 DEG C, and the time is
20-30min, pH 6.8-7.5.
6. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The power of the microwave is
400-600W, frequency 800-1000MHZ.
7. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The low temperature organic solvent leaching
Carry the ethanol solution that organic solvent in step is a concentration of 80-95%.
8. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The low temperature organic solvent leaching
Temperature in step is put forward to control at 50-60 DEG C.
9. the preparation method of ganoderma active polysaccharide according to claim 1, it is characterised in that：The enzymolysis liquid purification process
The material composition of composite decoloring agent and its parts by weight are in step：9-15 parts of atlapulgite, 5-8 parts of beta-cyclodextrin, citicoline
1-2 parts, the additive amount of the composite decoloring agent is the 3-5% of ganoderma lucidum crude polysaccharide concentrate.
10. ganoderma active polysaccharide prepared by the preparation method of the ganoderma active polysaccharide described in claim 1-9 any one.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110746518A (en) * | 2019-12-11 | 2020-02-04 | 西藏天虹科技股份有限责任公司 | Preparation method of high-purity ganoderma lucidum polysaccharide |
| CN114767581A (en) * | 2022-03-14 | 2022-07-22 | 珠海市日新化妆品有限公司 | Method for preparing ganoderma lucidum extract by using ganoderma lucidum residue and application |
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| CN101701041A (en) * | 2009-12-02 | 2010-05-05 | 安发（福建）生物科技有限公司 | Technology for extracting ganoderma active polysaccharide at low-temperature |
| CN104292357A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Extraction method of ganoderma lucidum polysaccharide |
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2018
- 2018-05-02 CN CN201810408292.0A patent/CN108424472A/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701041A (en) * | 2009-12-02 | 2010-05-05 | 安发（福建）生物科技有限公司 | Technology for extracting ganoderma active polysaccharide at low-temperature |
| CN104292357A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Extraction method of ganoderma lucidum polysaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110746518A (en) * | 2019-12-11 | 2020-02-04 | 西藏天虹科技股份有限责任公司 | Preparation method of high-purity ganoderma lucidum polysaccharide |
| CN114767581A (en) * | 2022-03-14 | 2022-07-22 | 珠海市日新化妆品有限公司 | Method for preparing ganoderma lucidum extract by using ganoderma lucidum residue and application |
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