CN102048655A - Method for extracting asparagus polysaccharides from discarded asparagus and application of asparagus polysaccharides - Google Patents
Method for extracting asparagus polysaccharides from discarded asparagus and application of asparagus polysaccharides Download PDFInfo
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Abstract
The invention relates to a method for extracting asparagus polysaccharides from discarded asparagus and an application of the asparagus polysaccharides, belonging to the technical field of biochemical engineering. The asparagus polysaccharides can be applied in cosmetics and pharmaceutical health care products. The method comprises the following steps: mixing the discarded asparagus with ethanol, stirring, and centrifugating, thus obtaining asparagus residue without impurities; further using the ultrasonic circulating extraction technology to extract the asparagus polysaccharides from the asparagus residue at normal temperature, concentrating extraction solution of the polysaccharides, then mixing with an extracting agent for deproteinization, stirring, and centrifugating; then adding ethanol into supernatant fluid for precipitation, drying precipitate, adding water for dissolution, performing chromatographic separation on a DEAE (diethylaminoethyl) cellulose ion exchange column, and collecting, and then performing concentration, alcohol precipitation and drying, thus obtaining the refined asparagus polysaccharides. The method provided by the invention has the advantages that raw material sources are abundant, and the cost is low; the efficiency is high, and the time is short; the extraction at the normal temperature is adopted, thus the defects of degradation of the polysaccharides and activity decline caused by the traditional hot water extraction method of the polysaccharides is overcome; and the process flow is simple, the equipment investment is low, a solvent can be recovered and reused during the production process, and no three wastes can be generated.
Description
Technical field
The present invention relates to utilize the method for Germinatus Phragmitis leftover bits and pieces extraction Germinatus Phragmitis polysaccharide and the application of Germinatus Phragmitis polysaccharide, belong to the biochemical engineering technical field.
Background technology
Germinatus Phragmitis (Asparagus officinalis L.) is the perennial perennial root herbaceous plant of Liliaceae Asparagus.Germinatus Phragmitis is a kind of medicinal and famous and precious vegetable that eat of integrating, and is one of the world's ten big famous dishes, and is edible with tender stem, quality is fresh and tender, and the local flavor deliciousness is tender good to eat, the laudatory title that " king of vegetable " arranged in the world, its consumption figure is only second to Fructus Lycopersici esculenti, Semen Canavaliae and mushroom in Europe.Being classified as first of the vegetable that the world ten has nutritive value greatly most by World Health Organization (WHO), is the cancer-preventing health vegetable of generally acknowledging in the world, and the Germinatus Phragmitis consumption figure is also increasing year by year fast.
Germinatus Phragmitis contains various active composition (as organized enzyme, Germinatus Phragmitis polysaccharide, saponin, fatty acid, aminoacid, trace element etc.) and has special biological functions such as antitumor, defying age, resisting fatigue, blood fat reducing, protecting liver and detoxication, immunomodulating.In addition, contain abundant polysaccharide in the Germinatus Phragmitis, Fang Youlan has reported that the pure product of Germinatus Phragmitis polysaccharide are made up of two kinds of different polysaccharide of relative molecular mass, average molecular mass is 85000 and 14500, the glycosyl that constitutes polysaccharide mainly contains xylose, fucose and fructose, mol ratio is 1.0: 5.07: 8.97 (Fujian Normal University's journal, 1995,11 (2): 69-73); Ji Yubin has reported that the main monosaccharide of asparagus polysaccharides consists of xylose, fructose, rhamnose, arabinose, galactose, its mass fraction is respectively 3.144%, 7.92%, 10.52%, 17.15%, 41.85% (Chinese herbal medicine, 2006,37 (8): 1159-1161).Zhang Zhi's (Zhengzhou Husbandry Engineering Higher Training School's journal far away, 2003,23 (2): 83-84) and Miao Mingsan (Chinese Medicine journal, 2003,18 (1): 52-53) adopt peritoneal macrophage to engulf method, hemolysin and hemolysis plaque forming method and the lymphocyte transformation method is observed the influence of Germinatus Phragmitis polysaccharide to normal mouse immune effect.Find that the Germinatus Phragmitis polysaccharide can improve the Turnover of Mouse Peritoneal Macrophages phagocytic function, promote the formation of hemolysin, hemolysis plaque, promote lymphocytic conversion ratio.Draw the morning cutting edge of a knife or a sword and reported that also the Germinatus Phragmitis polysaccharide by improving S180 mouse red blood cell immunoregulation function, strengthens lymphocyte immunity respond (Shanghai Univ. of Traditional Chinese Medicine's journal, 2010,24 (2): 68-71).Miao Mingsan observes the Germinatus Phragmitis polysaccharide causes mouse aging to the D-galactose influence, find that the Germinatus Phragmitis polysaccharide can significantly improve mouse aging blood CAT, SOD, the GSH-PX vigor, atrophy (the CHINA JOURNAL OF CHINESE MATERIA MEDICA of thymus, spleen and cerebral tissue due to reduction blood plasma, brain, liver homogenate LPO level and the antagonism aging, 2004,29 (7): 673-675).Shen Meishu causes the mice that declines in order to Germinatus Phragmitis polysaccharide water decoction treatment D-galactose, find the reduction of Germinatus Phragmitis polysaccharide energy antagonism mouse aging thymus index, increase the Bcl-2 protein expression, reduce Fas protein expression level in the mouse aging thymic tissue, has certain anti-aging effects (Chinese food and nutrition, 2010,3:74-76).Patent CN101559157 discloses a kind of method of utilizing Germinatus Phragmitis slag grading extraction Germinatus Phragmitis saponin and Germinatus Phragmitis polysaccharide, this method is a raw material with the Germinatus Phragmitis slag after squeezing the juice, with hot ethanol reflux, extract, Germinatus Phragmitis saponin, again with residue with hot water molten lixiviate Germinatus Phragmitis polysaccharide, but it can not overcome traditional length consuming time that the hot water extraction polysaccharide process brought, highly energy-consuming, low-yield, high temperature to problems such as polysaccharide degradation effect and polysaccharide biological activity are destroyed.
Vegetable polysaccharides has diversified bioactive functions, and functional food and being extensive use of clinically, makes the research of vegetable polysaccharides become focus in recent years.Up to the present, existing more than 300 kind of polysaccharide compound separated from natural product, and the water soluble polysaccharide that wherein extracts from plant is the most important.These polysaccharide have extremely important and special physiologically active, have as immunomodulating, antitumor, blood sugar lowering, blood fat reducing, radioprotective, antiviral, antiinflammatory, resisting fatigue, the anti-ageing bioactive functions of waiting for a long time.The polysaccharide that present people successfully will extract from nearly hundred kind of plant is widely used in the research and development of health food and medicine.
Ultrasound wave is the sound wave of frequency more than 20KHz, and medium is produced unique mechanical vibration effect and cavitation.When ultrasonic vibration, can produce and transmit powerful energy, cause that medium quickens to enter vibrational state with big speed.The medium structure is changed, impel effective ingredient to enter in the solvent, simultaneously, ultrasound wave also can produce cavitation in liquid.Cavitation bubble rose big and breaks in moment, the sound field energy that absorbs when breaking discharges in extremely short time and minimum space, form the environment of high temperature and high pressure, be attended by powerful shock wave and little sound wave simultaneously, thereby destruction cell wall structure, it was broken in moment, and the active ingredient in the plant cell is released, and directly enters solvent and fully mixing.In addition, ultrasound wave also produces many second order effects such as heat effect, emulsifying, spreads, smashes, chemical effect, biological effect, agglomeration effect etc. also can be quickened the diffusion of active ingredients of plants in solvent and discharge, and is beneficial to extraction.Extract the method for polysaccharide with routine and compare, ultrasonic extraction can shorten extraction time, saves the energy, improves extraction ratio.
The existing planting asparagus area of China reaches 1,700,000 mu, produces 137.6 ten thousand tons per year, ranks first in the world, and in the Germinatus Phragmitis course of processing, the leftover bits and pieces such as about 30～40% old stem and Radix Crotalariae szemoensis skin of having an appointment cause the great environmental pollution and the wasting of resources.With the Germinatus Phragmitis leftover bits and pieces is raw material, adopts the ultrasonic circulating extractive technique to separate the Germinatus Phragmitis polysaccharide, and polysaccharide is purified, is applied to cosmetics, Medicines and Health Product field, can turn waste into wealth, and reaches the purpose of development and use waste resource.
Summary of the invention
The objective of the invention is in order to solve length consuming time that traditional hot water extraction polysaccharide process brought, highly energy-consuming, low-yield, high temperature the method that proposes to utilize the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide and the application of Germinatus Phragmitis polysaccharide to polysaccharide degradation effect and the ruined problem of polysaccharide biological activity.
The objective of the invention is to be achieved through the following technical solutions.
The application of Germinatus Phragmitis polysaccharide of the present invention, the Germinatus Phragmitis polysaccharide is applied in the cosmetics, in the gross mass of cosmetics, the Germinatus Phragmitis polysaccharide accounts for 0.1%～50% of cosmetics gross mass, and the method for its application is: the using method according to cosmetics before mixing behind Germinatus Phragmitis polysaccharide and the cosmetics mix homogeneously is used.
The Germinatus Phragmitis polysaccharide is applied in the cosmetics, can reach the effect of preserving moisture, whiten, smoothing away wrinkles.
The application of Germinatus Phragmitis polysaccharide of the present invention, the Germinatus Phragmitis polysaccharide is applied in the Medicines and Health Product, gross mass in Medicines and Health Product, the Germinatus Phragmitis polysaccharide accounts for 0.1%～95% of Medicines and Health Product gross mass, and the method for its application is: the using method according to Medicines and Health Product before mixing behind Germinatus Phragmitis polysaccharide and the Medicines and Health Product mix homogeneously is used.
The Germinatus Phragmitis polysaccharide is applied in the Medicines and Health Product, can play free radical, defying age, the auxiliary effect of administering of cancer of removing.
The method of utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide of the present invention, concrete steps are:
1) the Germinatus Phragmitis leftover bits and pieces being pulverized the back, to add concentration be 70%～85% ethanol, heats then and stirs impurity such as removal saponin, monosaccharide, pigment;
Particle diameter after above-mentioned Germinatus Phragmitis leftover bits and pieces is pulverized is 40～60 orders; Heating-up temperature is 40～60 ℃;
Above-mentioned Germinatus Phragmitis leftover bits and pieces is 1g: 3～5ml with alcoholic acid ratio;
2) Germinatus Phragmitis slag-alcohol mixture is carried out solid-liquid separation, then with the Germinatus Phragmitis slag 40～50 ℃ of oven dry, and ethanol reclaimed;
3) the Germinatus Phragmitis slag after will drying adds water, puts into the ultrasonic circulating extraction element and extracts, and extraction conditions is ultrasonic power 100～1000W, dutycycle 1～5: 1,30～60 ℃ of temperature, after the liquid-solid separation the Germinatus Phragmitis liquid of extracting polysaccharide;
Above-mentioned water is 20～40ml: 1g with the ratio of Germinatus Phragmitis slag, and extraction time is 30～50min;
4) the Germinatus Phragmitis liquid of extracting polysaccharide is evaporated to 1/5～1/3 of Germinatus Phragmitis liquid of extracting polysaccharide volume;
5) liquid of extracting polysaccharide after concentrating adds extractant, stirs, and centrifugal layering gets three layers of liquid of upper, middle and lower, and the upper strata is a polysaccharide solution, and the intermediate layer is a protein solution, and lower floor is an extractant;
Above-mentioned extractant is the mixture of chloroform and n-butyl alcohol; Chloroform and n-butyl alcohol volume ratio are 4～5: 1;
Above-mentioned mixing time is 2～5h;
6) remove the intermediate layer, the upper strata polysaccharide solution is mixed with the lower floor extractant, stir 2～5h, centrifugal layering;
7) repeating step 6) 3～10 times, purity of polysaccharide reaches 60%～70%;
8) will add ethanol in the polysaccharide solution of upper strata, polysaccharide precipitates from solution, and centrifugalize gets Germinatus Phragmitis polysaccharide, vacuum drying then; Ethanol can reuse;
Above-mentioned polysaccharide solution and ethanol mix the back concentration of ethanol more than or equal to 80%; Above-mentioned vacuum drying temperature is 40～50 ℃;
9) the Germinatus Phragmitis polysaccharide that step 8) is obtained is dissolved in water, and is mixed with concentration and is 1%～3% aqueous solution, carries out chromatography on DEAE cellulose ion exchange column, concentrates after the collection, precipitate with ethanol, drying, obtains the Germinatus Phragmitis polysaccharide.
Beneficial effect
Raw materials for production of the present invention are the Germinatus Phragmitis leftover bits and pieces, and raw material sources are abundant, and cost is low; Employed ultrasonic circulating extractive technique has improved the yield of Germinatus Phragmitis polysaccharide, has shortened extraction time, and simultaneously, extract at room temperature has overcome polysaccharide degraded and the active defective that descends that traditional hot water extraction polysaccharide causes; Technological process is simple, equipment investment is few, and the recycling of production process solvent recovery does not have " three wastes " and produces, and both energy-saving and water-saving was free from environmental pollution again; The Germinatus Phragmitis polysaccharide can reach the effect of preserving moisture, whiten, smoothing away wrinkles as cosmetic applications; The Germinatus Phragmitis polysaccharide is used as Medicines and Health Product, can play free radical, defying age, the auxiliary effect of administering of cancer of removing.
The specific embodiment
Embodiment 1
Utilize the Germinatus Phragmitis leftover bits and pieces to extract the method for Germinatus Phragmitis polysaccharide, concrete steps are:
1) 100g Germinatus Phragmitis leftover bits and pieces is crushed to 40 orders, adds 400ml concentration then and be 70% ethanol, be heated to 40 ℃ and stir then, remove impurity such as saponin, monosaccharide, pigment;
2) Germinatus Phragmitis slag-alcohol mixture is carried out solid-liquid separation, then with the Germinatus Phragmitis slag 45 ℃ of oven dry, and ethanol reclaimed;
3) the Germinatus Phragmitis slag after will drying adds water, puts into the ultrasonic circulating extraction element and extracts, and extraction conditions is ultrasonic power 100W, dutycycle 1: 1,30 ℃ of temperature, after the liquid-solid separation the Germinatus Phragmitis liquid of extracting polysaccharide;
Above-mentioned water is 40ml: 1g with the ratio of Germinatus Phragmitis slag, and extraction time is 30min;
4) the Germinatus Phragmitis liquid of extracting polysaccharide is evaporated to 1/4 of Germinatus Phragmitis liquid of extracting polysaccharide volume;
5) liquid of extracting polysaccharide after concentrating adds extractant, stirs, and centrifugal layering gets three layers of liquid of upper, middle and lower, and the upper strata is a polysaccharide solution, and the intermediate layer is a protein solution, and lower floor is an extractant;
Above-mentioned extractant is the mixture of chloroform and n-butyl alcohol; Chloroform and n-butyl alcohol volume ratio are 4: 1; Mixing time is 5h;
6) remove the intermediate layer, the upper strata polysaccharide solution is mixed with the lower floor extractant, stir 2h, centrifugal layering;
7) repeating step 6) 3 times, purity of polysaccharide is 65%;
8) will add ethanol in the polysaccharide solution of upper strata, polysaccharide precipitates from solution, and centrifugalize gets Germinatus Phragmitis polysaccharide, vacuum drying then; Ethanol can reuse;
It is 85% that above-mentioned polysaccharide solution and ethanol mix the back concentration of ethanol; Above-mentioned vacuum drying temperature is 50 ℃;
9) the Germinatus Phragmitis polysaccharide that step 8) is obtained is dissolved in water, and is mixed with concentration and is 3% aqueous solution, carries out chromatography on DEAE cellulose ion exchange column, concentrates after the collection, precipitate with ethanol, drying, obtains Germinatus Phragmitis polysaccharide 3g.
The above-mentioned Germinatus Phragmitis polysaccharide that obtains is applied in the cosmetics, gross mass in cosmetics, the Germinatus Phragmitis polysaccharide accounts for 1% of cosmetics gross mass, and the method for its application is: with being coated in cheek portion behind Germinatus Phragmitis polysaccharide and the cosmetics mix homogeneously, play the effect of preserving moisture, whiten, smoothing away wrinkles.
The above-mentioned Germinatus Phragmitis polysaccharide that obtains is applied in the Medicines and Health Product, gross mass in Medicines and Health Product, the Germinatus Phragmitis polysaccharide accounts for 5% of Medicines and Health Product gross mass, the method of its application is: the using method according to Medicines and Health Product before mixing behind Germinatus Phragmitis polysaccharide and the Medicines and Health Product mix homogeneously is used, played free radical, defying age, the auxiliary effect of administering of cancer of removing.
Embodiment 2
Utilize the Germinatus Phragmitis leftover bits and pieces to extract the method for Germinatus Phragmitis polysaccharide, concrete steps are:
1) 100g Germinatus Phragmitis leftover bits and pieces is crushed to 60 orders, adds 500ml concentration then and be 85% ethanol, be heated to 60 ℃ and stir then, remove impurity such as saponin, monosaccharide, pigment;
2) Germinatus Phragmitis slag-alcohol mixture is carried out solid-liquid separation, then with the Germinatus Phragmitis slag 50 ℃ of oven dry, and ethanol reclaimed;
3) the Germinatus Phragmitis slag after will drying adds water, puts into the ultrasonic circulating extraction element and extracts, and extraction conditions is ultrasonic power 100W, dutycycle 5: 1,60 ℃ of temperature, after the liquid-solid separation the Germinatus Phragmitis liquid of extracting polysaccharide;
Above-mentioned water is 20ml: 1g with the ratio of Germinatus Phragmitis slag, and extraction time is 50min;
4) the Germinatus Phragmitis liquid of extracting polysaccharide is evaporated to 1/3 of Germinatus Phragmitis liquid of extracting polysaccharide volume;
5) liquid of extracting polysaccharide after concentrating adds extractant, stirs, and centrifugal layering gets three layers of liquid of upper, middle and lower, and the upper strata is a polysaccharide solution, and the intermediate layer is a protein solution, and lower floor is an extractant;
Above-mentioned extractant is the mixture of chloroform and n-butyl alcohol; Chloroform and n-butyl alcohol volume ratio are 5: 1; Mixing time is 5h;
6) remove the intermediate layer, the upper strata polysaccharide solution is mixed with the lower floor extractant, stir 2h, centrifugal layering;
7) repeating step 6) 10 times, purity of polysaccharide is 70%;
8) will add ethanol in the polysaccharide solution of upper strata, polysaccharide precipitates from solution, and centrifugalize gets Germinatus Phragmitis polysaccharide, vacuum drying then; Ethanol can reuse;
It is 87% that above-mentioned polysaccharide solution and ethanol mix the back concentration of ethanol; Above-mentioned vacuum drying temperature is 50 ℃;
9) the Germinatus Phragmitis polysaccharide that step 8) is obtained is dissolved in water, and is mixed with concentration and is 2% aqueous solution, carries out chromatography on DEAE cellulose ion exchange column, concentrates after the collection, precipitate with ethanol, drying, obtains Germinatus Phragmitis polysaccharide 3.3g.
The above-mentioned Germinatus Phragmitis polysaccharide that obtains is applied in the cosmetics, gross mass in cosmetics, the Germinatus Phragmitis polysaccharide accounts for 10% of cosmetics gross mass, and the method for its application is: with being coated in cheek portion behind Germinatus Phragmitis polysaccharide and the cosmetics mix homogeneously, play the effect of preserving moisture, whiten, smoothing away wrinkles.
The above-mentioned Germinatus Phragmitis polysaccharide that obtains is applied in the Medicines and Health Product, gross mass in Medicines and Health Product, the Germinatus Phragmitis polysaccharide accounts for 85% of Medicines and Health Product gross mass, the method of its application is: the using method according to Medicines and Health Product before mixing behind Germinatus Phragmitis polysaccharide and the Medicines and Health Product mix homogeneously is used, played free radical, defying age, the auxiliary effect of administering of cancer of removing.
Claims (10)
1. the application of Germinatus Phragmitis polysaccharide is characterized in that: the Germinatus Phragmitis polysaccharide is applied in the cosmetics, and in the gross mass of cosmetics, the Germinatus Phragmitis polysaccharide accounts for 0.1%～50% of cosmetics gross mass.
2. the method used of Germinatus Phragmitis polysaccharide is characterized in that: with behind Germinatus Phragmitis polysaccharide and the cosmetics mix homogeneously according to the using method use that mixes preceding cosmetics, reach the effect of preserving moisture, whiten, smoothing away wrinkles.
3. the application of Germinatus Phragmitis polysaccharide is characterized in that: the Germinatus Phragmitis polysaccharide is applied in the Medicines and Health Product, and in the gross mass of Medicines and Health Product, the Germinatus Phragmitis polysaccharide accounts for 0.1%～95% of Medicines and Health Product gross mass.
4. the method used of Germinatus Phragmitis polysaccharide is characterized in that: use according to the using method of Medicines and Health Product before mixing behind Germinatus Phragmitis polysaccharide and the Medicines and Health Product mix homogeneously, play the effect that free radical, defying age, cancer are assisted improvement of removing.
5. utilize the Germinatus Phragmitis leftover bits and pieces to extract the method for Germinatus Phragmitis polysaccharide, it is characterized in that concrete steps are:
1) the Germinatus Phragmitis leftover bits and pieces being pulverized the back, to add concentration be 70%～85% ethanol, heats then and stir;
Above-mentioned Germinatus Phragmitis leftover bits and pieces is 1g: 3～5ml with alcoholic acid ratio;
2) Germinatus Phragmitis slag-alcohol mixture is carried out solid-liquid separation, then with the Germinatus Phragmitis slag 40～50 ℃ of oven dry;
3) the Germinatus Phragmitis slag after will drying adds water, put into the ultrasonic circulating extraction element and extract, after the liquid-solid separation the Germinatus Phragmitis liquid of extracting polysaccharide; Water is 20～40ml: 1g with the ratio of Germinatus Phragmitis slag;
4) Germinatus Phragmitis liquid of extracting polysaccharide concentrating under reduced pressure;
5) liquid of extracting polysaccharide after concentrating adds extractant, stirs, and centrifugal layering gets three layers of liquid of upper, middle and lower, and the upper strata is a polysaccharide solution, and the intermediate layer is a protein solution, and lower floor is an extractant;
Above-mentioned extractant is the mixture of chloroform and n-butyl alcohol; Mixing time is 2～5h;
6) remove the intermediate layer, the upper strata polysaccharide solution is mixed with the lower floor extractant, stir 2～5h, centrifugal layering;
7) repeating step 6) 3～10 times, purity of polysaccharide reaches 60%～70%;
8) will add ethanol in the polysaccharide solution of upper strata, polysaccharide precipitates from solution, and centrifugalize gets Germinatus Phragmitis polysaccharide, vacuum drying then;
9) the Germinatus Phragmitis polysaccharide that step 8) is obtained is dissolved in water, and is mixed with concentration and is 1%～3% aqueous solution, carries out chromatography on DEAE cellulose ion exchange column, concentrates after the collection, precipitate with ethanol, drying, obtains the Germinatus Phragmitis polysaccharide.
6. the method for utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide according to claim 5 is characterized in that: the particle diameter after the Germinatus Phragmitis leftover bits and pieces is pulverized in the step 1) is 40～60 orders; Heating-up temperature is 40～60 ℃.
7. the method for utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide according to claim 5, it is characterized in that: extraction conditions is ultrasonic power 100～1000W in the step 3), dutycycle 1～5: 1,30～60 ℃ of temperature, extraction time is 30～50min.
8. the method for utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide according to claim 5, it is characterized in that: the Germinatus Phragmitis liquid of extracting polysaccharide is evaporated to 1/5～1/3 of Germinatus Phragmitis liquid of extracting polysaccharide volume in the step 4).
9. the method for utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide according to claim 5, it is characterized in that: chloroform and n-butyl alcohol volume ratio are 4～5: 1 in the step 5).
10. the method for utilizing the Germinatus Phragmitis leftover bits and pieces to extract the Germinatus Phragmitis polysaccharide according to claim 5 is characterized in that: polysaccharide solution and ethanol mix the back concentration of ethanol more than or equal to 80% in the step 8); The vacuum drying temperature once had been 40～50 ℃.
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| CN102432691A (en) * | 2011-12-12 | 2012-05-02 | 辽宁仙鹤矿泉水有限公司 | Method for extracting polysaccharides from reed rhizome |
| CN105558203A (en) * | 2016-01-21 | 2016-05-11 | 赵洪军 | Healthcare tea capable of reducing blood pressure and preparation process thereof |
| CN105639006A (en) * | 2016-01-16 | 2016-06-08 | 赵洪军 | Blood fat reducing healthcare tea and production process thereof |
| CN105707220A (en) * | 2016-01-29 | 2016-06-29 | 赵洪军 | Apple preservative preparation method |
| CN110467686A (en) * | 2019-09-17 | 2019-11-19 | 齐鲁工业大学 | A kind of highly effective extraction method of Root of Asparagus officinalis polysaccharide |
| CN113637093A (en) * | 2021-09-18 | 2021-11-12 | 四川工大西南食品研究有限责任公司 | Method for extracting and separating polysaccharide and saponin from asparagus leftovers |
| CN114276472A (en) * | 2022-01-13 | 2022-04-05 | 南京昂谷医药科技有限公司 | Preparation process of asparagus polysaccharide |
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| CN102432691A (en) * | 2011-12-12 | 2012-05-02 | 辽宁仙鹤矿泉水有限公司 | Method for extracting polysaccharides from reed rhizome |
| CN105639006A (en) * | 2016-01-16 | 2016-06-08 | 赵洪军 | Blood fat reducing healthcare tea and production process thereof |
| CN105558203A (en) * | 2016-01-21 | 2016-05-11 | 赵洪军 | Healthcare tea capable of reducing blood pressure and preparation process thereof |
| CN105707220A (en) * | 2016-01-29 | 2016-06-29 | 赵洪军 | Apple preservative preparation method |
| CN110467686A (en) * | 2019-09-17 | 2019-11-19 | 齐鲁工业大学 | A kind of highly effective extraction method of Root of Asparagus officinalis polysaccharide |
| CN110467686B (en) * | 2019-09-17 | 2021-03-30 | 齐鲁工业大学 | Efficient extraction method of asparagus root polysaccharide |
| CN113637093A (en) * | 2021-09-18 | 2021-11-12 | 四川工大西南食品研究有限责任公司 | Method for extracting and separating polysaccharide and saponin from asparagus leftovers |
| CN114276472A (en) * | 2022-01-13 | 2022-04-05 | 南京昂谷医药科技有限公司 | Preparation process of asparagus polysaccharide |
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