CN100434434C - Method for extracting high purity stachyose from radix rehmanniae recen and producing prepared rehmannia root - Google Patents
Method for extracting high purity stachyose from radix rehmanniae recen and producing prepared rehmannia root Download PDFInfo
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- CN100434434C CN100434434C CNB2004100629541A CN200410062954A CN100434434C CN 100434434 C CN100434434 C CN 100434434C CN B2004100629541 A CNB2004100629541 A CN B2004100629541A CN 200410062954 A CN200410062954 A CN 200410062954A CN 100434434 C CN100434434 C CN 100434434C
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- stachyose
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- rehmannia
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- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 title claims abstract description 40
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 30
- 241000405414 Rehmannia Species 0.000 title abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 239000000463 material Substances 0.000 claims description 15
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- 239000008103 glucose Substances 0.000 claims description 6
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- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 claims description 4
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- YTBSYETUWUMLBZ-QWWZWVQMSA-N D-threose Chemical compound OC[C@@H](O)[C@H](O)C=O YTBSYETUWUMLBZ-QWWZWVQMSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
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- 241000405911 Rehmannia glutinosa Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- FLUADVWHMHPUCG-OVEXVZGPSA-N Verbascose Natural products O(C[C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](OC[C@@H]2[C@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]3(CO)[C@H](O)[C@@H](O)[C@@H](CO)O3)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 FLUADVWHMHPUCG-OVEXVZGPSA-N 0.000 description 1
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- LHAOFBCHXGZGOR-NAVBLJQLSA-N alpha-D-Manp-(1->3)-alpha-D-Manp-(1->2)-alpha-D-Manp Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@@H](O[C@@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1 LHAOFBCHXGZGOR-NAVBLJQLSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
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- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
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- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
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- FLUADVWHMHPUCG-SWPIJASHSA-N verbascose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)O3)O)O2)O)O1 FLUADVWHMHPUCG-SWPIJASHSA-N 0.000 description 1
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Abstract
The present invention relates to a method for extracting high purity stachyose from raw rehmannia and preparing prepared rehmannia from residual substances of extracted stachyose through processing and reduction, and belongs to the field of biological medicine preparation. The present invention is characterized in that the raw rehmannia is used as raw materials; the high purity stachyose is extracted from the rehmannia through methods of pure water leaching, rough filtration, fine filtration, purification, etc.; residual substances in each process are collected and partial saccharide substances are added; after mixing, prepared rehmannia which can fully meet pharmacopoeia requirements by a wine stewing method. The present invention has the advantages of simple and advanced manufacturing process, and great market potential.
Description
The present invention relates to a kind of high purity stachyose that extracts from Radix Rehmanniae, the surplus materials that has extracted behind the stachyose can produce Radix Rehmanniae Preparata after the processing reduction, belong to field of biological pharmacy.
Technical field
A kind of method of extracting high purity stachyose and production Radix Rehmanniae Preparata from Radix Rehmanniae of indication of the present invention belongs to field of biological pharmacy.Present method not only can extract high purity stachyose from Radix Rehmanniae, its purity reaches as high as more than 90%, has extracted the surplus materials behind the stachyose, through the processing reduction, also can be made into Radix Rehmanniae Preparata.Stachyose is that a kind of of discovered in recent years has the oligose of plurality kinds of health care effect to human body, and health-care effect that it is superior and potential using value more and more are subjected to the favor in market.Glutinous rehmannia is known as " mother of Chinese medicine " for the beneficial simply complementary class Chinese medicine that everybody knows, and use is all arranged in plurality of Chinese patent medicine, and glutinous rehmannia has the branch of Radix Rehmanniae, Radix Rehmanniae Preparata, and both all can be used as medicine, but the range of application of Radix Rehmanniae Preparata is wider, and application quantity is bigger.Present method has not only extracted high purity stachyose from Radix Rehmanniae, also realized the suitability for industrialized production of Radix Rehmanniae Preparata, not only glutinous rehmannia has been carried out comprehensive development and utilization, and to the Chinese medicine large-scale production, realizes that the modernization of Chinese medicine all has better guide significance.
Background technology
Glutinous rehmannia (Rehmannia glutinosa Libosch) is scrophulariaceae (Scrophulariaceae) plant, also is the principal item of Chinese medicine, is the beneficial complementary class Chinese medicine of using always, and the beginning is stated from Shennong's Herbal.Glutinous rehmannia is divided into Rehmannia Root, Radix Rehmanniae and Radix Rehmanniae Preparata.That digs out annual September to October is Rehmannia Root, Rehmannia Root through cure or directly airing after water content is lower than 20%, become Radix Rehmanniae; Radix Rehmanniae is a Radix Rehmanniae Preparata through the wine steaming or after the stewed system of water.The main pharmacological of Rehmannia Root is heat-clearing, promote the production of body fluid, cool blood, appetizing etc.Radix Rehmanniae is heat-clearing, cooling blood for hemostasis etc.Radix Rehmanniae Preparata is a nourishing YIN and supplementing blood, and beneficial essence is filled out marrow etc.Because the Rehmannia Root preservation period is shorter, pharmacological action is similar with Radix Rehmanniae.
By now, found more than 60 kind of composition, but main functional component is glucoside material and glucide from glutinous rehmannia.Contain the 23 kinds of glucoside materials of having an appointment in the glutinous rehmannia, wherein based on iridoid glycosides.Glucide isolation identification has gone out 8 kinds, is respectively stachyose, raffinose, glucose, sucrose, fructose, mannotriose, verbascose and semi-lactosi.Other also contain amino acids material, mineral ion and trace element.
Contain various saccharides in the glutinous rehmannia, the wherein relevant stachyose that mainly contains with its effect, RPS-a (consisting of of glycogen: pectinose-rhamnosyl-glucose-seminose 4: 4: 2: 1), (consisting of of glycogen: semi-lactosi-glucose-wood sugar-seminose-pectinose 12: 6: 2: 2: 1), these glucides all can strengthen the immunization of human body to RPS-b.
But experiment is found, glutinous rehmannia is in the process of slaking, the total reducing sugar amount does not have significant difference (about 70%), after the stachyose that content is the highest in the glutinous rehmannia (reaching as high as 60%) is concocted, content obviously reduces (about 17%), and fructose (preceding 3.26% back 9.23%), glucose (preceding 1.56%, the back 8.57%) content of forming Dihuang polysaccharide RPS-a, RPS-b increase.Therefore glutinous rehmannia most polysaccharide in the process of concocting is converted into monose, if under the prerequisite of not destroying other compositions in the glutinous rehmannia, superfluous water threose in the Radix Rehmanniae is put forward with isolating method, the specific interpolation of surplus materials RPS-a, RPS-b, then can reach ripe glutinous rehmannia, this is a key point of the present invention.
Stachyose (Stachyose) is a kind of novel functional oligose, and more research has been done in its exploitation as functional foodstuff or pharmaceutical raw material at home and abroad.The stachyose structural formula is semi-lactosi-semi-lactosi-glucose-fructose, and molecular formula is C
24H
42O
21Molecular weight is 666.59, because connect with α-D-semi-lactosi glycosidic bond between glucosyl group and 2 semi-lactosis in its molecule, enzyme-the α-D-Ban Rutangganmei that lacks this key of hydrolysis in the human body, so it can not be absorbed by human consumption, the intravital most of flora of people does not have this kind of enzyme system yet, therefore can not utilize stachyose, only residing in minority probioticss such as bifidus bacillus in the enteron aisle and Lactobacterium acidophilum has this enzyme system, so stachyose has just become the specificity of these probioticss short biological.The stachyose efficacy factor is clear and definite, structure is clear, be described as third generation functional oligose, improving human gastrointestinal tract function, enhance immunity ability, preventing that cholesterol from raising, promoting that all many-sides such as mineral substance absorption, preventing dental caries all have many potential purposes.
Summary of the invention
The invention provides a kind of method of extracting stachyose and producing Radix Rehmanniae Preparata from Radix Rehmanniae, its summary of the invention mainly comprises the method for extracting stachyose from Radix Rehmanniae and the method for producing Radix Rehmanniae Preparata.
Now existing a plurality of patents have been mentioned the production method of stachyose, but it just puts forward the stachyose in the plant by variety of way, and remaining slag charge is not made full use of, so not only wasted resource, and polluted environment greatly, all costs all have been added among the stachyose, make the cost of stachyose high, and market competition does not have advantage.The characteristics of present method are not only to have extracted stachyose from glutinous rehmannia, and surplus materials processing has been reduced into Radix Rehmanniae Preparata, not only made full use of the natural resources of Chinese medicinal materials of this preciousness of glutinous rehmannia, and the cost of stachyose and Radix Rehmanniae Preparata is all significantly reduced, improved the market competitiveness of stachyose and Radix Rehmanniae Preparata greatly, through data-searching, also do not find similar extracting method, the present invention has the advance and the practicality of its uniqueness.
Description of drawings
The technological process of this method sees Figure of description for details. In the method, radix rehmanniae recen is that fresh rehmannia root is excavated by naturally drying and gets, Maximum temperature in the dry process is no more than 50 ℃. Extracting used water after pulverizing need be through purifying. Extraction time about 4 to 8 is little The time, adopt membrane separation process to decolour. In the whole stachyose leaching process, do not add any harmful material. Each The surplus materials of operation section must the remanufacture prepared rhizome of rehmannia. Additive is glucide, comprises arabinose, rhamnose, Portugal Grape sugar, mannose, galactolipin, wood sugar etc. All substances will mix before wine was stewed, adopt yellow rice wine to carry out wine and stew, yellow rice wine with The ratio of mixture is 30～50: 100. Wine is stewed to black profit, and oven dry is prepared rhizome of rehmannia.
The stachyose purity of producing through the method reaches as high as more than 90%, and the black profit of prepared rhizome of rehmannia color, slightly aroma, flavor are sweet, warp Detect, indices all meets the requirement of pharmacopeia 2000 editions.
Concrete embodiment
Embodiment 1:
Getting 100 kilograms of Radix Rehmanniae dry products cleans, pulverize with pulverizer, use the pure water after ion exchange treatment to soak 5 hours for 500 kilograms, leach liquor is filtered to transparent clarification with the plate basket, use hollow-fibre membrane (0.5 micron in aperture) to filter again, 400 kilograms of the liquid of transparent, little Huang.This method can be separated the water-soluble components such as stachyose in the glutinous rehmannia with other compositions.Surplus materials after residue after the extraction, coarse filtration, the smart filter all will be collected, in order to producing the usefulness of Radix Rehmanniae Preparata.
Above-mentioned 400 kilograms of liquid are made about 50 kilograms of pressed powder with the spray-dired method of decompression, dissolve this powder with 300 kilogram 75% alcohol, and insoluble substance is about 30 kilograms, and this is the crude product stachyose, and its purity is about 50%.Standby after the surplus materials drying behind the recovered alcohol.
Utilize membrane filter method that the impurity in the crude product stachyose is removed, purity is 25 kilograms of 94% stachyoses.
Collect about 65 kilograms of surplus materials in each technological process, add 25 kilograms of pectinoses, rhamnosyl, glucose, seminose, its ratio is 4: 4: 2: 1.Mix and dry, get 85 kilograms in mixture.The purpose that this process is added 4 kinds of glucides is the deficiency of supplementing carbohydrate.
Soak said mixture with 32 kilograms of yellow rice wine, and coddle system, to stew to the wine exhaustion, oven dry promptly gets 85 kilograms of Radix Rehmanniae Preparata, and this Radix Rehmanniae Preparata is deceived profit, flavor is sweet, glossy, reaches the every requirement of officinal fully.
Embodiment 2:
After getting 500 kilograms of Radix Rehmanniaes cleanings, be crushed to about 5mm with pulverizer, soaked 7 hours for 2000 kilograms with the pure water after ion exchange treatment, leach liquor is put into whizzer and is filtered, its rotating speed is 1000 rev/mins, use millipore filter (0.5 micron in aperture) to filter again, 1500 kilograms of the liquid of transparent, little Huang.
Above-mentioned 1500 kilograms of liquid are made about 225 kilograms of pressed powder with the spray-dired method of subtraction, are that 1000 kilogram 80% alcohol dissolves this powder repeatedly with total amount, insoluble substance be about 180 kilograms, this is the crude product stachyose, its purity is 56%.Standby after the surplus materials drying behind the recovered alcohol.
Utilize fermentation method that the impurity in the crude product stachyose is removed, purity is 150 kilograms of 97% stachyoses.
Collect about 300 kilograms of surplus materials in each technological process, add 140 kilograms of semi-lactosis, glucose, wood sugar, seminose, pectinose (12: 6: 2:: 2: 1), mix and dry, 430 kilograms in mixture.
Soak said mixture with 100 kilograms of yellow rice wine, and coddle system, to stew to the wine exhaustion, oven dry promptly gets 430 kilograms of Radix Rehmanniae Preparata, and this Radix Rehmanniae Preparata reaches the every requirement of officinal after testing fully.
Claims (1)
1, a kind of method of extracting stachyose and producing Radix Rehmanniae Preparata from Radix Rehmanniae is characterized in that: 1) Radix Rehmanniae is used the pure water lixiviate, and coarse filtration, smart filter, purifying obtains stachyose; 2) surplus materials behind the collection extraction stachyose, adding proportion is 4: 4: 2: 1 pectinose, rhamnosyl, glucose, seminose or adding proportion are 12: 6: 2: 2: 1 semi-lactosi, glucose, wood sugar, seminose, pectinose, mix the stewed oven dry of back wine and obtain Radix Rehmanniae Preparata.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100629541A CN100434434C (en) | 2004-07-01 | 2004-07-01 | Method for extracting high purity stachyose from radix rehmanniae recen and producing prepared rehmannia root |
Applications Claiming Priority (1)
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| CN100457765C (en) * | 2007-01-16 | 2009-02-04 | 广东太阳神集团有限公司 | Method for producing stabhyose, and method for producing stabhyose and catalpol by using rehmannia root |
| CN101649335B (en) * | 2009-06-25 | 2012-02-15 | 中国食品发酵工业研究院 | Preparation method of high-purity alpha-1,6 trisaccharide |
| CN102028801B (en) * | 2009-09-30 | 2013-03-20 | 上海中医药大学附属曙光医院 | Radix Rehmanniae oligosaccharide pulmonary delivery traditional Chinese medicinal preparation as well as preparation method and application thereof |
| CN103319546B (en) * | 2013-06-18 | 2016-05-04 | 陕西新药技术开发中心 | A kind of from glutinous rehmannia the method for Separation of Water threose |
| CN104059111B (en) * | 2014-05-19 | 2016-08-24 | 邱建国 | The method extracting manninotriose monomer from glutinous rehmannia with activated carbon gradient elution |
| CN105315314A (en) * | 2014-07-31 | 2016-02-10 | 中国食品发酵工业研究院 | Industrial production method for extracting stachyose from radix rehmanniae |
| CN104262418B (en) * | 2014-09-26 | 2017-04-19 | 何龙 | Production method of crystallized water threose |
| CN112426429B (en) * | 2020-11-19 | 2022-09-27 | 河南中医药大学 | Carbohydrate composition for nourishing yin and supplementing blood for yin and blood deficiency syndrome |
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Non-Patent Citations (4)
| Title |
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| 功能性低聚糖---水苏糖. 梁丽心.中国食品添加剂，第4期. 2004 |
| 功能性低聚糖——水苏糖. 梁丽心.中国食品添加剂，第4期. 2004 * |
| 地黄中寡糖的提取分离工艺研究. 张汝学等.解放军药学学报，第21卷第1期. 2005 |
| 地黄中寡糖的提取分离工艺研究. 张汝学等.解放军药学学报，第21卷第1期. 2005 * |
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