CN108403613B

CN108403613B - Camel milk wash-free mask with antibacterial and acne removing effects and preparation method thereof

Info

Publication number: CN108403613B
Application number: CN201810561463.3A
Authority: CN
Inventors: 吉日木图; 郝甜; 何静; 明亮
Original assignee: Camel Research Institute In Inner Mongolia
Current assignee: Inner Mongolia Tiantuo Biotechnology Co ltd
Priority date: 2018-06-04
Filing date: 2018-06-04
Publication date: 2020-03-31
Anticipated expiration: 2038-06-04
Also published as: CN108403613A

Abstract

The invention relates to a camel milk no-clean mask with antibacterial and acne-removing effects and a preparation method thereof. The camel milk wash-free mask consists of camel milk whey, a humectant, an antioxidant, a film forming agent, a softening agent, an emulsifier, a thickening agent, triethanolamine, an essence, a preservative and deionized water. The camel milk no-clean mask disclosed by the invention is free of cleaning, is very convenient to use, provides rich nutrient substances for skin, has the characteristics of being warm and non-irritating, easy to absorb, strong in moisture retention, anti-inflammatory and antibacterial effects, and smooth, fine, moist, compact and full of vitality after being used.

Description

Camel milk wash-free mask with antibacterial and acne removing effects and preparation method thereof

[ technical field ] A method for producing a semiconductor device

The invention relates to the technical field of cosmetics. More specifically, the invention relates to a camel milk no-clean facial mask with antibacterial and acne-removing effects, and also relates to a preparation method of the camel milk no-clean facial mask with antibacterial and acne-removing effects.

[ background of the invention ]

Modern women need to endure a severe air environment except for intense work and family pressure, and the skin is extremely easy to lack water and age, and is also easy to cause acne and face skin allergy. The facial mask is covered on the face, temporarily isolates the pollution of the outside air within a short time, improves the temperature of the skin, expands pores of the skin, promotes the secretion and metabolism of cold evil, increases the oxygen content of the skin, and is beneficial to the skin to remove the metabolic products of surface cells and accumulated grease substances. The water in the facial mask permeates into the horny layer of epidermis, so that the skin becomes soft, and the skin is natural, bright and elastic. The sleeping mask is a mask which is applied on the face to sleep directly after basic skin care is finished at night, is convenient to use, and solves the problem that many women consume too much time when applying the mask.

Camel milk has been used as a traditional food and drink good product and medical cosmetics for people in arid desert regions, and has the reputation of 'gold in desert'. As early as 1982, camel milk has been regarded as a food with medical and health care effects for the folk because of the important role of its biological value in the disease resistance mechanism of the organism. In recent years, the medical health care function of camel milk is researched and developed.

Some research works show that camel milk is rich in various nutrient components beneficial to human health and is easy to digest and absorb by human bodies. Besides the similar nutrient components of other animal milks, the camel milk contains protective proteins such as lysozyme, peroxidase, lactoferrin and immunoglobulin, and has better content and structure than other animal milks, so the camel milk has stronger functions of sterilization, anti-allergy, immunity and the like. A plurality of animal experiments prove that the camel milk has the effects of hematopoiesis, blood circulation promotion, tranquilizing and allaying excitement, liver and spleen function enhancement, pancreas function improvement, liver and kidney nourishing, lung heat clearing and summer heat relieving, yin nourishing, detoxification, necrotic tissue removal and the like. The research also shows that the Kazak traditional fermented camel milk has obvious inhibition effect on mouse foot swelling caused by carrageenan and granuloma formation caused by cotton balls, and further has the functions of removing necrotic tissues, resisting inflammation and regulating immunity. Camel milk has been used for external application for wound disinfection, pain relief, inflammation diminishing and arthritis pain in Mongolia, Arab and some surrounding countries for a long time in folks, and has obtained good effects.

The nutrients in cow milk are of great interest in beauty. The current research shows that the contents of lactoferrin, immunoglobulin, nicotinic acid and vitamin C in camel milk are obviously higher than that of cow milk. The camel milk is complete in amino acid types, rich in essential amino acid content of human body, particularly the content of tryptophan (Trp) is obviously higher than that of milk, the camel milk also contains fruit acid (AHA) and salicylic acid (BHA), the camel milk is a natural source of the fruit acid and the salicylic acid, and the hydroxy acid is considered to have the effects of enabling the skin to be smooth and fine. The camel milk is superior to natural beauty components of other animal milks, and is proved to be a good beauty product given by God.

Acne (Acne) is a chronic inflammatory disease that occurs in hair follicles, sebaceous glands. The pathogenesis of the acne vulgaris comprises various reasons, wherein microbial infection is a more important reason, and researches show that the acne vulgaris is subjected to Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidisCocci, etc. Western medicine treatment of acne vulgaris can inhibit and reduce sebum secretion, inhibit Propionibacterium acnes, control inflammation, and avoid the destruction of hair follicle sebaceous gland structure. In general terms, adverse element stimulation, such as irritancy, high sugar, high fat foods; improper use of cosmetics, mechanical damage, climatic environmental factors, and care should be taken for facial cleansing. Systemic acne medication comprises: applying antibiotics such as milbemycin, tetracycline, clindamycin, Michler's disease, azithromycin, etc.; antiandrogenic drugs such as dyne-35, others such as spironolactone, H₂Receptor antagonists, and the like. The application of drugs for inhibiting abnormal keratinization of pilosebaceous canal can relieve sore, such as retinoid. Other medicines, such as tanshinone capsule, have antibacterial and anti-inflammatory effects, and can promote skin metabolism and inhibit sebaceous gland. Research shows that the tanshinone capsule also has mild estrogen activity and can resist androgen to some extent.

The camel milk contains protective proteins such as lysozyme, peroxidase, lactoferrin and immunoglobulin, the content and the structure of the camel milk are superior to those of other livestock milks, the antibacterial and anti-inflammatory effects of the camel milk are undoubtedly superior to those of other milks, and the camel milk is a natural antibacterial and anti-inflammatory auxiliary treatment substance without any side effect and is popular with people when being used in antibacterial and acne-removing cosmetics.

[ summary of the invention ]

[ problem to be solved ]

The invention aims to provide a camel milk no-clean mask with bacteriostatic and acne-removing effects.

The invention also aims to provide a preparation method of the camel milk no-clean facial mask with the effects of inhibiting bacteria and removing acnes.

[ solution ]

The invention is realized by the following technical scheme.

The invention relates to a camel milk no-clean facial mask with antibacterial and acne removing effects.

The camel milk wash-free mask comprises the following components: in parts by weight

According to a preferred embodiment of the present invention, the humectant is one or more selected from glycerin, propylene glycol, sodium hyaluronate, 1, 3-butylene glycol, ethylhexylglycerin or trehalose.

According to another preferred embodiment of the invention, the antioxidant is tocopheryl acetate.

According to another preferred embodiment of the invention, the film-forming agent is polydimethylsiloxane.

According to another preferred embodiment of the invention, said emollient is one or more emollients selected from caprylic/capric triglyceride, squalane or isopropyl palmitate.

According to another preferred embodiment of the invention, the emulsifier is one or more emulsifiers selected from the group consisting of glyceryl monostearate, cetearyl alcohol, tween 80 or castor oil.

According to another preferred embodiment of the invention, the thickener is one or more thickeners selected from xanthan gum, carbomer or hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer.

According to another preferred embodiment of the present invention, the preservative is one or more preservatives selected from phenoxyethanol, methylisothiazolinone, or methylparaben.

According to another preferred embodiment of the present invention, the essence is one or more essence selected from fantasy orchard essential oil or gardenia essential oil.

The invention also relates to a preparation method of the camel milk leave-in mask.

The preparation method comprises the following steps:

A. preparing an oil phase mixture:

stirring and mixing 2-5 parts by weight of film forming agent, 0.5-3.0 parts by weight of softening agent and 0.5-1.5 parts by weight of emulsifier at 75-80 ℃ until the components are completely dissolved to obtain an oil phase mixture;

B. preparation of an aqueous phase mixture:

adding deionized water into 6-12 parts by weight of humectant for dissolving; adding deionized water into a mixture of 0.5-3.0 parts by weight of a thickening agent and 0.1-0.5 part by weight of triethanolamine to dissolve; mixing the two solutions, and stirring at 75-80 ℃ until the two solutions are completely dissolved to obtain a water phase mixture;

C. mixing

Pouring the water phase mixture obtained in the step B into the oil phase mixture obtained in the step A under the condition of stirring, continuously stirring for 2-5 min at the stirring speed of 15-25 r/min, and then homogenizing for 2-5 min at the homogenizing speed of 5000-6000 r/min and the temperature of 60-70 ℃ by using a homogenizer to obtain a mixture;

D. charging of

And D, reducing the temperature of the mixture obtained in the step C to room temperature, adding 10-30 parts by weight of camel milk whey, 0.05-0.5 part by weight of antioxidant, 0.1-0.3 part by weight of essence and 0.05-1.0 part by weight of preservative, supplementing 100 parts by weight of deionized water, continuously homogenizing for 5-10 min at the homogenizing speed of 8000-12000 r/min by using a homogenizer, and then cooling to room temperature, thus obtaining the camel milk no-clean facial mask with the antibacterial and acne-removing effects.

The present invention will be described in more detail below.

The inventor develops the sleeping mask containing natural camel milk components and having the effects of moisturizing and resisting inflammation on the basis of the research on various nutrient substances which are beneficial to the skin of camel milk according to the characteristics of the camel milk. It has been found that the addition of active principles extracted from camel milk to a composition containing a moisturizing and stabilizing base allows the active principles to be in intimate contact with the skin, not only to promote better absorption by the skin, but also to reduce waste. Therefore, the camel milk leave-in mask disclosed by the invention is a sleeping mask containing effective components of camel milk.

The camel milk (camel milk) contains main nutrient components including protein, fat, carbohydrate, vitamins, minerals and the like, and is a good product for beautifying. Camel milk contains various bioactive substances such as casein, lactoferrin, immunoglobulin, vitamins and the like, is a main component of camel milk with antibacterial and anti-inflammatory effects, and although milk of other animals also contains the components, the content of camel milk is obviously lower, for example, the vitamin C content of 100ml camel milk and the vitamin C content of equivalent cow milk are respectively 3.8mg and 1.0 mg. Camel milk also contains fruit acids (AHA) and salicylic acid (BHA), which are natural sources of fruit acids and salicylic acid, while hydroxy acids are believed to have the effect of making the skin round, smooth, and fine. Camel milk is superior to other natural cosmetic components of animal milk.

The camel milk whey used in the invention is a separated product obtained by separating and removing milk fat and casein by stirring and acid precipitation. The preparation method of the camel milk whey comprises the following steps:

centrifuging fresh camel milk at 4 deg.C and 5000r/min for 30min, filtering to separate milk fat, adjusting pH of the filtrate to isoelectric point 4.3 with hydrochloric acid solution, standing for 30min, centrifuging for 20min, and removing casein to obtain camel milk whey.

In the invention, when other components are in the content range, if the camel milk whey content is less than 10 parts by weight, the prepared mask has poor moisturizing effect; if the camel milk whey content is higher than 30 parts by weight, the prepared mask has higher cost and is not easy to absorb; therefore, the camel milk content is preferably 10 to 30 parts by weight, more preferably 12 to 20 parts by weight, and still more preferably 13 to 18 parts by weight.

In the invention, the emulsifier has the basic function of reducing the interfacial surface tension of the emulsion to reduce the potential energy of a dispersion system, so that the stability of the camel milk no-clean mask paste is improved, and water cannot be analyzed out easily.

The emulsifier used in the present invention is one or more emulsifiers selected from the group consisting of glyceryl monostearate, cetearyl alcohol, tween-80 or hydrogenated lecithin.

The glyceryl monostearate, cetearyl alcohol, Tween-80 or hydrogenated lecithin emulsifiers are all currently marketed products, such as glyceryl monostearate sold under the trade name "glyceryl stearate monoglyceride" by the british procury company, cetearyl alcohol sold under the trade name hexastearyl alcohol by the cantonese company, Tween-80 sold under the trade name Tween-80 by the shanghai reagent company, hydrogenated lecithin sold under the trade name "hydrogenated lecithin" by the youco company.

In the present invention, when the other components are within the above content range, if the emulsifier content is less than 0.5 part by weight, the paste is unstable and water and oil are easily separated; if the emulsifier content is more than 1.5 parts by weight, the cost is high and uneconomical; therefore, the emulsifier is suitably contained in an amount of 0.5 to 1.5 parts by weight, preferably 0.7 to 1.3 parts by weight, more preferably 0.8 to 1.0 part by weight.

In the invention, the basic function of the emollient is to increase the lubricating property of the camel milk no-clean mask cream, so that the skin can feel smooth and silky.

The emollient used in the present invention is one or more selected from caprylic/capric triglyceride, squalane or isopropyl palmitate.

The caprylic/capric triglyceride, squalane and isopropyl palmitate emollients are all products currently on the market, such as caprylic/capric triglyceride sold under the trade name "caprylic/capric triglyceride" by the German winning company, natural squalane sold under the trade name "squalane" by the Japanese oil company, and isopropyl palmitate sold under the trade name "isopropyl palmitate" by the American International publication company.

In the present invention, when the other components are within the content range, if the content of the emollient is less than 0.5 part by weight, the absorption effect of the nutrient of the mask is not good; if the content of the softening agent is higher than 3.0 parts by weight, the mask is too greasy; therefore, the content of the softening agent is suitably 0.5 to 3 parts by weight, preferably 0.5 to 1.5 parts by weight, more preferably 0.8 to 1.2 parts by weight.

In the invention, the film forming agent has the basic functions of increasing the film forming property of the camel milk no-clean mask paste, and increasing smooth and greasy skin, and is not greasy and has no residual feeling.

The film-forming agent used in the present invention is polydimethylsiloxane. The polydimethylsiloxane film former is a product currently marketed, for example, by the Youso company under the trade name "Dimethicone".

In the present invention, when the other components are within the content range, if the content of the film-forming agent is less than 2 parts by weight, the formed film is thin and is not easily peeled off; if the content of the film forming agent is more than 5 parts by weight, flocculent gel can be formed, and homogeneous sol can not be formed; therefore, the content of the film forming agent is suitably 2 to 5 parts by weight, preferably 3 to 5 parts by weight, more preferably 3.5 to 4.5 parts by weight.

In the present invention, the moisturizer basically functions to help the skin absorb moisture and prevent moisture loss inside the skin.

The humectant used in the present invention is one or more selected from glycerin, propylene glycol, sodium hyaluronate, 1, 3-butylene glycol, ethylhexylglycerin or trehalose.

The glycerin, propylene glycol, sodium hyaluronate, 1, 3-butanediol, and ethylhexylglycerin moisturizers are commercially available products, and examples thereof include glycerin sold under the trade name of glycerin by Qingdao Yousioka, propylene glycol sold under the trade name of "propylene glycol" by Qingdao Yousioka, sodium hyaluronate sold under the trade name of low molecular sodium hyaluronate by Shandong Furuida, 1, 3-butanediol sold under the trade name of "1, 3-butanediol" by Qingdao Yousioka, ethylhexylglycerin sold under the trade name of "ethylhexylglycerin" by Qingdao Yousioka, and trehalose sold under the trade name of D-trehalose imported from Japan.

In the present invention, when the other components are within the content range, if the content of the humectant is less than 6 parts by weight, the moisturizing effect is not significant; if the content of the humectant is higher than 12 parts by weight, the paste is too greasy and the air permeability is not good; therefore, it is reasonable that the content of the humectant is 5 to 12 parts by weight, preferably 7 to 10 parts by weight, more preferably 8 to 9 parts by weight.

In the invention, the thickening agent basically has the function of enabling the camel milk no-clean mask paste to be in a semitransparent paste shape and enabling the paste to keep stable.

The thickener used in the present invention is one or more thickeners selected from xanthan gum, carbomer or hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer.

The xanthan gum, carbomer, and hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer thickeners are all currently marketed products, such as carbomer sold under the trade name "carbomer 540" by shanghai agents, xanthan gum sold under the trade name xanthan gum by Qingdao Yousio, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer sold under the trade name EMT 10 by Guangzhou Kongpo.

In the present invention, when the other components are within the content range, if the content of the thickener is less than 0.5 parts by weight, the prepared mask is relatively diluted and does not form a paste; if the thickener content is more than 3.0 parts by weight, the paste is too thick to spread and has uneven texture; therefore, it is feasible that the thickener is contained in an amount of 0.5 to 3 parts by weight, preferably 1 to 2 parts by weight, more preferably 1.2 to 1.8 parts by weight.

In the present invention, the preservative essentially serves to render the camel milk leave-on mask of the present invention microbiologically stable and of sufficient shelf life.

The preservative used in the present invention is one or more preservatives selected from phenoxyethanol, methylisothiazolinone, or methylparaben.

The self-phenoxyethanol, methylisothiazolinone, and methylparaben preservatives are commercially available products, such as phenoxyethanol sold under the trade name "phenoxyethanol" by Qingdao Yousio, methylisothiazolinone sold under the trade name Kathon (Kathon) by Qingdao Yousio, and methylparaben sold under the trade name methylparaben by Kyowa, Guangzhou.

In the present invention, when the other components are within the content range, if the content of the preservative is less than 0.05 parts by weight, the preservative effect is not significant; if the content of the preservative is more than 1.0 part by weight, there is a side effect on the skin; therefore, it is feasible that the content of the preservative is 0.05 to 1.0 part by weight, preferably 0.1 to 0.8, more preferably 0.2 to 0.6.

In the invention, the antioxidant basically has the function of enabling the camel milk wash-free mask to have stronger reducibility and enabling the mask to have the functions of resisting oxidation and preventing aging.

The antioxidant used in the present invention is tocopherol acetate, which is a product currently marketed, for example, by the company Qingdao under the name tocopherol acetate.

In the present invention, when the other components are within the content range, if the antioxidant content is less than 0.05 parts by weight, the antioxidant effect is insignificant; if the antioxidant content is more than 0.5 part by weight, the color is easily changed, and the appearance of the mask is influenced; therefore, it is feasible that the antioxidant is contained in an amount of 0.05 to 0.5 parts by weight, preferably 0.08 to 0.45, more preferably 0.15 to 0.30.

In the invention, the triethanolamine has the basic functions of neutralizing carbomer and thickening so that the pH value of the camel milk mask reaches a specified range.

The triethanolamine used in the present invention is a product currently marketed, for example, by the Qingdao Yousio company under the trade name triethanolamine.

In the invention, when other components are in the content range, if the content of the neutralizer is less than 0.1 part by weight, the pH value of the camel milk mask is too small, and the acidity is too large; if the triethanolamine content is higher than 0.5 part by weight, the pH value is too high, and the viscosity of the mask is high, so that the mask does not meet the requirements; therefore, a triethanolamine content of 0.1 to 0.5 parts by weight is feasible, preferably 0.18 to 0.40, more preferably 0.25 to 0.32.

The essence used in the invention is one or more essences selected from fantasy orchard essential oil or gardenia essential oil. They are all products currently on the market, such as fantasy orchard essence sold under the trade name "fantasy orchard essence" by Qingdao Yousio, and gardenia essential oil sold under the trade name "gardenia essential oil" by Qingdao Yousio.

The amount of the essence used in the present invention is 0.1 to 0.3 part by weight, and it is obvious that too much or too little amount of the essence is disadvantageous.

The invention also relates to a preparation method of the camel milk moisturizing sleeping mask.

The preparation method comprises the following steps:

A. preparing an oil phase mixture:

B. preparation of an aqueous phase mixture:

C. mixing

D. charging of

The camel milk no-clean mask disclosed by the invention is white and slightly bright in color, fine and smooth in texture, free of stimulation to skin and capable of achieving good effects of moisturizing.

The camel milk whey, the humectant, the antioxidant, the film forming agent, the emollient, the emulsifier, the thickener, the essence, the preservative, the triethanolamine and the like used in the preparation method are described in detail in the foregoing, and thus are not described in detail herein.

The equipment used in the preparation of the invention is the equipment which is generally used in the technical field and sold in the market at present.

In the invention, the dosage and the physicochemical properties of the used raw materials generate a coordination effect, the pH value of the camel milk no-clean facial mask is close to that of human skin, and the camel milk no-clean facial mask has no irritation to the skin. Therefore, after the face is cleaned every night, the camel milk no-clean facial mask is smeared on the face, the camel milk no-clean facial mask can fall asleep directly without being cleaned, and the camel milk no-clean facial mask is preferably used before eleven points at night.

[ advantageous effects ]

The invention has the beneficial effects that: the camel milk leave-in facial mask disclosed by the invention contains natural camel milk whey which has the effects of resisting oxidation, bacteria and inflammation, contains abundant vitamins, amino acids and mineral substances and provides nutrient substances for skin, and also contains fruit acid (AHA) and salicylic acid (BHA), and the hydroxy acid is considered to have the effects of enabling the skin to be smooth and fine. The moisture-preserving sleep mask disclosed by the invention is stable in preparation process, white in color, slightly bright, fine in texture and free of stimulation to skin, and achieves good effects of preserving moisture, moistening and removing acne.

[ description of the drawings ]

FIG. 1 is a measurement result of the moisturizing rate of camel milk leave-on facial masks prepared in examples 1 to 4 of the invention;

FIG. 2 is a skin moisture measurement result of the camel milk leave-on mask prepared in examples 1 to 4 of the present invention;

FIG. 3 is a graph showing the results of measuring the content of TNF- α;

FIG. 4 is a graph showing the results of IL-8 assay;

FIG. 5 is a comparison of abscess of rabbit ear molding (left panel) and post-treatment (right panel);

fig. 6 is a comparison of rabbit ear pore molding (left panel) and post-treatment (right panel).

FIG. 7 is a graph of the effect of camel milk whey on bacteriostatic rate at different pH values;

fig. 8 is a graph of the effect of camel milk whey on bacteriostatic rate at different temperatures.

[ detailed description ] embodiments

The invention will be better understood from the following examples.

Example 1: preparation of camel milk wash-free mask

The implementation of this example is as follows:

A. preparing an oil phase mixture:

at a temperature of 75 ℃, stirring and mixing 2 parts by weight of polydimethylsiloxane film-forming agent, 2 parts by weight of caprylic/capric triglyceride emollient and 0.5 part by weight of glyceryl monostearate emulsifier until the materials are completely dissolved to obtain an oil phase mixture;

B. preparation of an aqueous phase mixture:

adding deionized water into 6 parts by weight of glycerol humectant for dissolving; adding deionized water into a mixture of 0.5 weight part of xanthan gum thickener and 0.1 weight part of triethanolamine for dissolution; mixing the two solutions, and stirring at 75 deg.C until the two solutions are completely dissolved to obtain an aqueous mixture;

C. mixing

Pouring the water phase mixture obtained in the step B into the oil phase mixture obtained in the step A under the condition of stirring, continuously stirring for 3min at the stirring speed of 15r/min, and then homogenizing for 3min at the homogenizing speed of 5400r/min and the temperature of 60 ℃ by using a homogenizer to obtain a mixture;

D. charging of

And D, reducing the temperature of the mixture obtained in the step C to room temperature, adding 16 parts by weight of camel milk whey obtained according to the method described in the specification, 0.1 part by weight of tocopherol acetate antioxidant, 0.1 part by weight of fantasy orchard essence and 0.05 part by weight of phenoxyethanol preservative, supplementing 100 parts by weight of deionized water, homogenizing for 8min at a homogenizing speed of 9000r/min by using a homogenizer, and then reducing the temperature to room temperature, thus obtaining the camel milk no-clean mask with the effects of inhibiting bacteria and removing acnes.

The test results of the camel milk no-clean mask prepared in the embodiment, such as physical and chemical index inspection, product sensory tests, skin irritation tests, moisture retention tests, skin moisture detection, rabbit ear acne model tests and the like, are shown in the following test embodiment part.

Example 2: preparation of camel milk wash-free mask

The implementation of this example is as follows:

A. preparing an oil phase mixture:

stirring and mixing 4 parts by weight of polydimethylsiloxane film former, 3 parts by weight of squalane emollient and 0.8 part by weight of emulsifier (0.3 part by weight of glyceryl monostearate +0.5 part by weight of cetearyl alcohol) at a temperature of 80 ℃ until all are dissolved to obtain an oil phase mixture;

B. preparation of an aqueous phase mixture:

adding deionized water into 8 parts by weight of propylene glycol humectant to dissolve; adding deionized water into a mixture of 1.4 parts by weight of carbomer thickener and 0.2 part by weight of triethanolamine for dissolution; mixing the two solutions, and stirring at 80 deg.C until the two solutions are completely dissolved to obtain an aqueous phase mixture;

C. mixing

Pouring the water phase mixture obtained in the step B into the oil phase mixture obtained in the step A under the condition of stirring, continuously stirring for 2min at the stirring speed of 20r/min, and then homogenizing for 2min at the homogenizing speed of 5000r/min and the temperature of 62 ℃ by using a homogenizer to obtain a mixture;

D. charging of

And D, reducing the temperature of the mixture obtained in the step C to room temperature, adding 30 parts by weight of camel milk whey obtained according to the method described in the specification, 0.3 part by weight of tocopherol acetate antioxidant, 0.2 part by weight of gardenia fragrance essential oil essence and 0.2 part by weight of methylisothiazolinone preservative, supplementing 100 parts by weight of deionized water, homogenizing for 5min at the homogenizing speed of 8000r/min by using a homogenizer, and then reducing the temperature to room temperature, thus obtaining the camel milk wash-free mask with the antibacterial and acne-removing effects.

Example 3: preparation of camel milk wash-free sleeping mask

The implementation of this example is as follows:

A. preparing an oil phase mixture:

stirring and mixing 5 parts by weight of polydimethylsiloxane film-forming agent, 1 part by weight of isopropyl palmitate emollient and 1.2 parts by weight of emulsifier (0.6 part by weight of glyceryl monostearate and 0.6 part by weight of tween 80) at the temperature of 75 ℃ until the components are completely dissolved to obtain an oil phase mixture;

B. preparation of an aqueous phase mixture:

adding deionized water into 12 parts by weight of humectant (6 parts by weight of glycerin, 3 parts by weight of propylene glycol, 0.8 part by weight of ethylhexyl glycerin, 0.2 part by weight of sodium hyaluronate and 2 parts by weight of trehalose) to dissolve; adding deionized water into a mixture of 2.2 parts by weight of a thickening agent (0.6 part by weight of xanthan gum +0.5 part by weight of carbomer +1.1 part by weight of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer) and 0.5 part by weight of triethanolamine for dissolution; mixing the two solutions, and stirring at 76 deg.C until the two solutions are completely dissolved to obtain an aqueous mixture;

C. mixing

Pouring the water phase mixture obtained in the step B into the oil phase mixture obtained in the step A under the condition of stirring, continuously stirring for 5min at the stirring speed of 25r/min, and then homogenizing for 4min at the homogenizing speed of 6000r/min and the temperature of 70 ℃ by using a homogenizer to obtain a mixture;

D. charging of

And D, reducing the temperature of the mixture obtained in the step C to room temperature, adding 24 parts by weight of camel milk whey obtained according to the method described in the specification, 0.5 part by weight of tocopherol acetate antioxidant, 0.3 part by weight of essence (0.1 dream orchard essential oil essence and 0.2 gardenia fragrance essential oil essence) and 0.6 part by weight of methylparaben preservative, supplementing 100 parts by weight of deionized water, continuously homogenizing for 10min at a homogenizing speed of 12000r/min by using a homogenizer, and reducing the temperature to room temperature to obtain the camel milk wash-free mask with the effects of inhibiting bacteria and removing acnes.

Example 4: preparation of camel milk wash-free sleeping mask

The implementation of this example is as follows:

A. preparing an oil phase mixture:

stirring and mixing 3 parts by weight of polydimethylsiloxane film former, 0.5 part by weight of caprylic/capric triglyceride emollient and 1.5 parts by weight of emulsifier (0.5 part by weight of cetearyl alcohol +1.0 part by weight of castor oil) at a temperature of 78 ℃ until all are dissolved to obtain an oil phase mixture;

B. preparation of an aqueous phase mixture:

adding deionized water into 10 parts by weight of humectant (5 parts by weight of glycerin, 3 parts by weight of propylene glycol, 0.5 part by weight of 1, 3-butanediol, 0.5 part by weight of ethylhexyl glycerin, 0.2 part by weight of sodium hyaluronate and 0.8 part by weight of trehalose) to dissolve; adding deionized water into a mixture of 3.0 parts by weight of a thickening agent (1.0 part by weight of xanthan gum +1.2 parts by weight of carbomer +0.8 part by weight of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer) and 0.4 part by weight of triethanolamine for dissolution; mixing the two solutions, and stirring at 78 deg.C until the two solutions are completely dissolved to obtain an aqueous mixture;

C. mixing

Pouring the water phase mixture obtained in the step B into the oil phase mixture obtained in the step A under the condition of stirring, continuously stirring for 4min at the stirring speed of 20r/min, and then homogenizing for 5min at the homogenizing speed of 5800r/min and the temperature of 66 ℃ by using a homogenizer to obtain a mixture;

D. charging of

And D, reducing the temperature of the mixture obtained in the step C to room temperature, adding 10 parts by weight of camel milk whey obtained according to the method described in the specification, 0.05 part by weight of tocopherol acetate antioxidant, 0.2 part by weight of gardenia fragrance essential oil essence and 1.0 part by weight of phenoxyethanol preservative, supplementing 100 parts by weight of deionized water, homogenizing for 7min at a homogenizing speed of 10000r/min by using a homogenizer, and reducing the temperature to room temperature to obtain the camel milk wash-free mask with the effects of inhibiting bacteria and removing acnes.

Test example 1: physical and chemical index inspection

The camel milk no-clean facial mask with the bacteriostatic and acne-removing effects, prepared in the embodiments 1-4, is subjected to the following tests and experiments:

1. trait detection

The camel milk no-clean mask disclosed by the invention is white and semitransparent, has the advantages of proper viscosity, proper hardness, fine and uniform paste body, good spreadability, coolness, comfort, no greasiness, good stability and fresh and elegant smell after being used.

2. Heat and cold resistance test

The camel milk no-clean mask is sealed in a transparent cosmetic bottle, and no layering phenomenon is observed and the state is unchanged after the camel milk no-clean mask is stored in a refrigerator at the temperature of-15 ℃ for 24 hours. After being placed in a constant temperature box with the temperature of 45 ℃ for 24 hours, the phenomena of layering, smell change, state change and the like do not occur.

3. Centrifugal test

The camel milk wash-free mask is packaged in a centrifuge tube with a plug, and is centrifuged for 30min at the rotating speed of 3000r/min, so that the phenomenon of layering does not occur.

4. Stability test

The camel milk wash-free mask is placed in a cosmetic bottle, and is kept stand at room temperature for 1 month for observation, so that the camel milk wash-free mask has no layering phenomenon, no change in feeling after use, and no change in smell.

5. pH detection

The pH value of the camel milk wash-free mask is measured by the pH value measuring method specified in GB/T13531.1-2008, and the detection result is shown in Table 1.

Table 1: the pH value measurement result of the camel milk no-clean mask

The results in table 1 show that the camel milk leave-on mask has a pH of 5.5-6.5 and meets the requirements of skin on acidity and alkalinity.

6. Viscosity detection

The viscosity values of the camel milk leave-on mask were measured using a digital display viscometer sold under the trade name NDJ-5 digital display by san feng technologies ltd, usa, and the measurement results are shown in table 2.

Table 2: the measurement result of the viscosity value of the camel milk wash-free mask

The results in table 2 show that the camel milk wash-free mask has moderate viscosity, is fine and smooth, is easy to spread and trowel, has a uniform film, and does not form small holes.

Test example 2: product sensory test

The camel milk no-clean mask with the bacteriostatic and acne-removing effects prepared in the embodiments 1-4 is tested for the using effect.

The specific implementation mode is as follows: the camel milk leave-on mask is distributed to 20 women of 20-50 years old for use, the camel milk leave-on mask is specified to be used for 2 times every week, and trial effect feedback is filled after the camel milk leave-on mask is used for 4 times.

The evaluation characteristics are defined as follows:

grading standard: adopting a ten-point system and a grading standard: the method is good: 9-10 minutes; preferably: 7-8 min; in general: 5-6 minutes; the worse is 3-4 minutes; very poor: 1-2 points, and the following items are the average points of the experimenters. The test results are shown in Table 3.

Table 3: the product sensory test result is as follows:

the results in table 3 clearly show that the camel milk leave-on mask prepared in example 2 has a higher overall sensory score and is more acceptable to the user.

Test example 3: skin irritation test

The test method comprises the following steps: the backs of 5 test mice were cut off, and the camel milk leave-in facial masks prepared in examples 1 to 4 were applied to the cut-off parts, respectively, and at the same time, the facial masks were applied to the parts without cut-off, for comparison. The test results are shown in table 4 below.

Table 4: results of skin irritation experiments

The results in table 4 show that the camel milk leave-on mask of the invention has no irritation to the skin of mice.

Then, 1g of the camel milk leave-in facial mask prepared in examples 1 to 4 was applied to the hands of 20 volunteers respectively for stimulation experiment verification, and no redness, rash, blistering and the like were observed after 30min of application, which proves that the camel milk leave-in facial mask has no stimulation and anaphylactic reaction to human skin.

Test example 4: moisture retention test of product

The leave-on camel milk facial masks prepared in examples 1 to 4 were subjected to moisture retention measurement.

The test method is as follows: the temperature of the constant temperature and humidity chamber is set at 25 ℃, the relative humidity is 80 percent, and the constant temperature and humidity chamber is kept stable for 12 hours for standby.

Sealing each culture dish by a preservative film, labeling, and weighing by an electronic balance to obtain M1. And (3) taking down the preservative film for sealing, uniformly smearing the camel milk wash-free mask on the bottom of a culture dish, and weighing to obtain M2. Putting the unsealed culture dish into a stable constant temperature and humidity box, taking out the culture dish at 4h, 8h and 24h respectively, sealing the culture dish by using the originally taken preservative film, and weighing to obtain M3. The moisture retention rate was calculated according to the following formula:

moisture retention rate (%) - (M3-M1)/(M2-M1) × 100%

The experimental results are shown in figure 1. As can be seen from the attached figure 1, the camel milk no-clean facial mask disclosed by the invention still has the moisture retention rate of over 50% after being exposed in the environment for 12 hours, and the moisture retention rate is still higher after 24 hours, so that the camel milk no-clean facial mask is good in moisture retention performance, can play a role in moisturizing and locking water for skin, and enables the skin to fully absorb moisture and nutrient substances.

Test example 5: skin moisture detection

The skin moisture content was measured before and after application of the camel milk leave-in mask prepared in examples 1 to 4 at 5min, 10min, 15min, 20min, 25min, 30min, 60min and 90min, respectively, using a digital skin moisture detector sold under the trade name digital skin moisture detector by Shenzhen Kaku silica gel electronics Limited.

The experimental results are shown in figure 2. As can be seen from the attached figure 2, after the camel milk leave-on facial mask is used, the moisture content of the skin rises instantly, then gradually drops due to the absorption effect of the skin on the nutrient components of the facial mask, and finally stabilizes at about 40%, so that the camel milk leave-on facial mask has a good moisturizing effect.

Test example 6: camel milk wash-free mask efficacy experiment

In order to verify the bacteriostatic acne-removing efficacy of the camel milk leave-on mask, the added whey has the bacteriostatic acne-removing efficacy, and a camel milk mask efficacy experiment of rabbit ears is carried out.

The test method comprises the following steps: the product is applied to rabbit ears suffering from acne twice a day, and is gently massaged for 5 minutes during application to ensure that substances can be fully absorbed, and is continuously applied for 30 days to observe the effect. The treatment group also comprises a vitamin A acid cream group for adjuvant treatment of acne vulgaris, a whey group and a camel milk facial mask group. The control group had no treatment for rabbit ears with acne.

The contents of IL-8 and TNF- α in rabbit ear skin tissues are measured according to a detection method of an interleukin-8 (IL-8) and tumor necrosis factor- α (TNF- α) kit, the antibacterial and acne-removing effects of the camel milk mask are tested by the change trend of the contents of interleukin-8 (IL-8) and tumor necrosis factor- α (TNF- α) in rabbit ear inflammation part tissues, and the results are shown in the attached figures 3 and 4.

As can be seen from the attached figures 3 and 4, the molding is basically successful after 14 days of molding, the rabbit ears suffer from acne, the experimental composition is applied, and the effect is obvious after 30 days of continuous application.

The results of the attached figures 3 and 4 can be compared to show that the IL-8 and TNF- α contents in the model group are obviously higher than those in the blank group, and the factor contents in the whey group and the camel milk facial mask group in the treatment group are obviously reduced, which shows that camel milk whey can effectively inhibit the release of IL-8 and TNF- α, so that the camel milk facial mask can be used for the adjuvant treatment of acne and has certain anti-bacterial and anti-acne effects.

The left graph in the attached figure 5 shows that the horny layer of rabbit ears is thickened, the skin surface is rough, the stratified squamous epithelium on the skin surface is obviously thickened, the dermis is formed with abscess and is broken to the skin surface, the peripheral blood vessels of the abscess are dilated, hyperemia and collagen fiber hyperplasia exist, and a large amount of neutral particles are infiltrated after the rabbit ears are molded for 14 days. FIG. 5 shows that the abscess and ulceration of the rabbit ear treated for 30 days is improved obviously, and hyperemia is basically disappeared;

the left picture of the attached figure 6 is a picture of pores of rabbit ears after molding, the surface is rough and is accompanied with exfoliation, hair follicles are expanded, and black angle plugs are arranged, and the right picture of the attached figure 6 is a picture of rabbit ear skin which is obviously improved, thinned and softened after the mask is smeared for 30 days, the pores are obviously reduced, and the black angle plugs are lightened to some extent.

The results of the attached figures 5 and 6 show that the camel milk mask can effectively relieve the symptoms of skin acne, relieve abscess, remove black keratotic plug, shrink hair follicles, play a role in moistening skin, can be used for adjuvant therapy of acne, and can play a role in resisting bacteria, resisting acne and moistening skin when being used by normal people.

Test example 7: camel milk whey in vitro bacteriostasis experiment

In order to further verify that the main material with the antibacterial and acne-removing effects of the camel milk leave-in mask is camel milk whey, and further study the influence of treatment at different pH values and different temperatures on the antibacterial effect of the camel milk whey through experiments, in-vitro antibacterial experiments on the camel milk whey are carried out.

The test method comprises the following steps: mixing camel milk whey powder and broth culture medium (the mass concentration of the camel milk whey powder is 20mg/mL), adjusting the pH values to 4.0, 5.0, 6.0, 7.0 and 8.0 by using 1mol/LHCI and 1mol/LNaOH respectively, and then inoculating propionibacterium acnes liquid; meanwhile, a blank control without adding camel milk whey powder is made at each pH value, a liquid culture medium containing camel milk whey at each pH value is used as a reference solution, anaerobic culture is carried out for 48 hours at 37 ℃, the OD value is measured, and the bacteriostasis rate at each pH value is calculated. Similarly, mixing the camel milk whey powder and the broth culture medium according to the method, placing the mixture in water baths with the temperatures of 30 ℃, 40 ℃, 50, 60, 70 and 80 ℃, preserving the heat for 5min, adding bacterial liquid after cooling the samples, and taking the mixed liquid treated in the water baths at the temperatures as reference solutions to obtain the bacteriostatic rate. The bacteriostatic rate was calculated as follows:

the bacteriostasis rate is 1- (A sample-A reference)/A blank multiplied by 100%

According to the bacteriostatic rate formula, the bacteriostatic rate of camel milk whey under different treatment conditions is calculated to judge the bacteriostatic performance, and the results are shown in the attached figures 7 and 8.

As can be seen from the results of FIG. 7, the pH difference has a significant effect on the inhibition rate. The camel milk whey has a certain inhibiting effect on propionibacterium acnes at the pH value of 4-8. When the pH value is between 4 and 6, the bacteriostatic rate of the camel milk whey on the propionibacterium acnes is gradually increased along with the increase of the pH value, and when the pH value is between 6 and 8, the bacteriostatic rate is gradually reduced, but the bacteriostatic effect is better than that of the camel milk whey, namely the pH value is between 4 and 6. The bacterial effect is better when the pH value is between 5.5 and 7.

As can be seen from the results in FIG. 8, the bacteriostatic effect of camel milk whey is reduced with the increase of the heat treatment temperature. When the temperature is lower than 60 ℃, the bacteriostasis rate is slowly reduced to be about 70%, and when the temperature is 60-80 ℃, the bacteriostasis rate is rapidly reduced to be about 15%.

The results of the attached figures 7 and 8 show that the camel milk whey has a good bacteriostatic action, the bacteriostatic rate of the camel milk whey is influenced by the pH value and the temperature, the bacteria effect is good when the pH value is 5.5-7, the influence on the bacteriostatic rate of the camel milk whey is not great when the treatment temperature is 40-50 ℃, and the camel milk whey can better exert the bacteriostatic action in the mask by combining the preparation process of the camel milk mask and the pH value index of the mask.

In conclusion, the camel milk facial mask prepared by the invention has the advantages of fine and uniform texture, good spreadability, refreshing and non-greasy after use, no stimulation, good moisturizing effect and certain acne removing effect. The long-term use of the skin-care product can not only achieve the effect of moistening the skin, but also effectively inhibit the growth of acnes, so that the skin can be kept in a healthy state for a long time.

Claims

1. A camel milk no-clean facial mask with antibacterial and acne removing effects is characterized by comprising the following components: in parts by weight

The humectant is one or more selected from propylene glycol, sodium hyaluronate, 1, 3-butanediol, ethylhexyl glycerin or trehalose; the antioxidant is tocopherol acetate; the film-forming agent is polydimethylsiloxane.

2. The camel milk leave-on mask of claim 1, wherein the emollient is one or more emollients selected from caprylic/capric triglyceride, squalane, or isopropyl palmitate.

3. The camel milk leave-on mask of claim 1, wherein the emulsifier is one or more emulsifiers selected from the group consisting of glyceryl monostearate, cetearyl alcohol, and castor oil.

4. The camel milk leave-on mask of claim 1, wherein the thickener is one or more thickeners selected from the group consisting of carbomers and hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymers.

5. The camel milk leave-on mask of claim 1, wherein the preservative is one or more preservatives selected from phenoxyethanol, methylisothiazolinone, or methylparaben.

6. The camel milk leave-on mask as claimed in claim 1, wherein the essence is one or more essence selected from fantasy orchard essential oil or gardenia essential oil.

7. The preparation method of the camel milk leave-on mask as claimed in any one of claims 1 to 6, characterized in that the method comprises the following steps:

A. preparing an oil phase mixture:

B. preparation of an aqueous phase mixture:

C. mixing

D. charging of

C, reducing the temperature of the mixture obtained in the step C to room temperature, adding 10-30 parts by weight of camel milk whey, 0.05-0.5 part by weight of antioxidant, 0.1-0.3 part by weight of essence and 0.05-1.0 part by weight of preservative, supplementing 100 parts by weight of deionized water, continuously homogenizing for 5-10 min by using a homogenizer at a homogenizing speed of 8000-12000 r/min, and then cooling to room temperature to obtain the camel milk no-clean mask with the effects of inhibiting bacteria and removing acnes;

the preparation method of the camel milk whey comprises the following steps: