CN108402002A - A kind of preparation method of fish bait - Google Patents
A kind of preparation method of fish bait Download PDFInfo
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- CN108402002A CN108402002A CN201810479595.1A CN201810479595A CN108402002A CN 108402002 A CN108402002 A CN 108402002A CN 201810479595 A CN201810479595 A CN 201810479595A CN 108402002 A CN108402002 A CN 108402002A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000001814 pectin Substances 0.000 claims abstract description 14
- 235000010987 pectin Nutrition 0.000 claims abstract description 14
- 229920001277 pectin Polymers 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims description 33
- 238000000855 fermentation Methods 0.000 claims description 24
- 230000004151 fermentation Effects 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 240000008042 Zea mays Species 0.000 claims description 18
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 18
- 238000005422 blasting Methods 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 16
- 235000005822 corn Nutrition 0.000 claims description 16
- 235000018102 proteins Nutrition 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- 235000001014 amino acid Nutrition 0.000 claims description 15
- 150000001413 amino acids Chemical class 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 13
- 238000007873 sieving Methods 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 241000723418 Carya Species 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 12
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 12
- 238000005292 vacuum distillation Methods 0.000 claims description 12
- 238000007792 addition Methods 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 235000013399 edible fruits Nutrition 0.000 claims description 9
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 7
- 244000223014 Syzygium aromaticum Species 0.000 claims description 7
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 6
- 238000001125 extrusion Methods 0.000 claims description 6
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical class CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 6
- 235000006109 methionine Nutrition 0.000 claims description 6
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 239000001294 propane Substances 0.000 claims description 6
- 235000010413 sodium alginate Nutrition 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 239000000661 sodium alginate Substances 0.000 claims description 3
- 229940005550 sodium alginate Drugs 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 239000003205 fragrance Substances 0.000 abstract description 6
- 235000019629 palatability Nutrition 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- 239000005667 attractant Substances 0.000 abstract description 3
- 230000031902 chemoattractant activity Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 abstract description 2
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 abstract description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract description 2
- 238000004090 dissolution Methods 0.000 abstract description 2
- 230000032050 esterification Effects 0.000 abstract description 2
- 238000005886 esterification reaction Methods 0.000 abstract description 2
- 230000004048 modification Effects 0.000 abstract description 2
- 238000012986 modification Methods 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000001035 drying Methods 0.000 description 5
- 230000008676 import Effects 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000036571 hydration Effects 0.000 description 3
- 238000006703 hydration reaction Methods 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 235000020995 raw meat Nutrition 0.000 description 3
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 229940108924 conjugated linoleic acid Drugs 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K97/00—Accessories for angling
- A01K97/04—Containers for bait; Preparation of bait
- A01K97/045—Preparation of bait; Ingredients
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of preparation methods of fish bait, belong to field of fodder.The present invention is by that can make pectin generate the sugar chain shorter, branch is less the modification of apple pectin soda acid, to reduce its molecular mass and esterification degree, increase its water solubility, and improve its galacturonic acid content, to preferably play its bioactivity and function, it can increase the bonding between food nutrition substance, be spilled into the water, and reduce resolution ratio, prevent fish Attractant from disperseing through water dissolution, without being searched for food by fish, the Edible qualified of bait is improved, preferable fish is obtained and obtains.The present invention solves poor, the not special fragrance of current fish bait palatability, the low problem of attracting effect.
Description
Technical field
The invention belongs to field of fodder, and in particular to a kind of preparation method of fish bait.
Background technology
With the very fast development of society, cause the operating pressure of people increasing, many people can select to fish
It is used as a kind of mode released stress." pole is joyful in hand." " pick up fishing rod, lose medicinal cupping." through the ages, people
A kind of outdoor sports of recreation and body-building all are treated as in fishing, not only can be with body-building, but also the heart can be good for.Fishing can get close to it is big from
So, full of life outdoor life temperament and interest is enjoyed, the pleasing beautiful scenery of lakes and mountains is had a taste of.Some researches show that the particle of bait is big
Small, shape, color and luster, temperature, hardness etc. can all become the prompt factor, and smell, taste, color, texture etc. provide judgement for fish
The foundation whether searched for food, these aspects all can be the factors for influencing to ingest.In recent years, with China's bait industry research and development ability
Enhancing, bait production technology level further increases, but the palatability of bait reduces in actual use, not special
Fragrance, attracting effect is very poor, cannot be obtained there are one good fish.Therefore, it is one to produce a kind of fish bait that palatability is good
The thing being highly desirable.
Invention content
The technical problems to be solved by the invention：For poor, the not special fragrance of current fish bait palatability, attracting effect
The low problem of fruit provides a kind of preparation method of fish bait.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of fish bait, which is characterized in that the preparation method includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 25 ~ 27 DEG C of activation cultures 10 ~ 12 days, obtains
Bacterium is activated, picking activation bacterium is seeded to by 3 ~ 5% inoculum concentration in strain fermentation culture medium, in 27 ~ 33 DEG C of cultures, obtains zymophyte
Liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 are added propane, and subcritical fluid extraction takes extract liquor decompression to steam
It evaporates, obtains vacuum distillation object, take corn protein powder steam blasting, obtain steam blasting object, according to the mass fraction, take 3 ~ 7 parts of zymophytes
Liquid, 20 ~ 30 parts of vacuum distillation objects, 150 ~ 200 parts of steam blasting objects, 2 ~ 5 parts of glucose, 0.3 ~ 0.8 part of bitter salt,
100 ~ 120 parts of water obtain fermentation culture medium in 25 ~ 28 DEG C of fermented and cultureds, spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 30 ~ 40min in 50 ~ 55 DEG C, obtain stirring liquid, take
Stir liquid in mass ratio 1：5 are added the sodium hydroxide solution of 3 mol/L, are stirred, are cooled to room temperature in 55 ~ 60 DEG C, adjust
PH to 3 ~ 3.5 is stood, and is taken and is stood liquid in mass ratio 1：2 ~ 5 are added then addition absolute ethyl alcohol, are stirred, filter, take precipitation
It is dry, obtain dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, takes 5 ~ 15 portions of fragrant fruits, 10 ~ 20 parts of asafoetides, 7 ~ 9 portions of cloves to dry, pulverize sieving, collects sieving particle, take sieving
Particle in mass ratio 1：50 are added distilled water, are stirred in 30 ~ 50 DEG C, centrifuge, take supernatant in mass ratio 5：3：3 additions are dry
Dry object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 200 ~ 300 parts of steps are taken（2）Spare fermentation culture medium, 50 ~ 70 parts of mixtures, 30 ~ 50 parts
Sodium alginate, 30 ~ 40 parts of Sodium Polyacrylates, 12 ~ 20 parts of glycine betaines, 60 ~ 80 parts of water mixing, are warming up to 55 ~ 60 DEG C and are stirred,
Mixture is obtained, extrusion is granulated, dry, obtains fish bait.
The step（1）Middle strain fermentation culture medium is according to the mass fraction, to take 8 ~ 10 parts of glucose, 1 ~ 3 part of seven hydration
Magnesium sulfate, 12 ~ 18 parts of potassium dihydrogen phosphates, 0.03 ~ 0.06 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizings
20min。
Compared with other methods, advantageous effects are the present invention：
（1）The present invention using corn protein powder as raw material, using hickory chick by high molecular weight protein Partial digestion be small molecular protein or
Polypeptide and amino acid, and hickory chick has abundant cellulase and lignoenzyme, can generate during the fermentation abundant
Enzyme system, can preferably act on corn protein powder, polysaccharide, protein in degrading maize albumen powder etc., carry out solid-state
It not will produce mycotoxin while fermented maize albumen powder, while fly maggot be added, fly maggot nutritional ingredient is comprehensive, is rich in protein
It is low with low sugar and fat content, and contain abundant mineral matter element, vitamin, trace element and antibacterial substance, nonreactive
Trophic factors and noxious material promote the palatability of bait, pass through subcritical fluid extraction unsaturated fatty acid, conjugated linoleic acid
Deng enriching the nutrient of bait；
（2）The present invention is by that can make pectin generate the sugar chain shorter, branch is less the modification of apple pectin soda acid, to reduce it
Molecular mass and esterification degree increase its water solubility, and improve its galacturonic acid content, to preferably play its bioactivity
And function, the bonding between food nutrition substance can be increased, be spilled into the water, resolution ratio is reduced, prevent fish Attractant from passing through
Water dissolution disperses, and without being searched for food by fish, improves the Edible qualified of bait, obtains preferable fish and obtain；
（3）The present invention is carried by the way that the substances such as l-Alanine, fragrant fruit, cloves are added using amino acid, the plant with fragranced
It taking object to have the characteristic of attractant effect to fish, bait is added, amino acid has the sense of taste of fish and smell extremely strong stimulation,
The taste that fish are liked attracts, and improves attracting effect, and fish searches for food close to bait, allows fish bite, improves fishing
Efficiency.
Specific implementation mode
Hickory chick：Me-01 is purchased from Jilin university wheat and corn deep processing laboratory.
Corn protein powder：The Jilin China Oil and Food Import and Export Corporation biochemistry energy sells Co., Ltd.
Strain fermentation culture medium：According to the mass fraction, take 8 ~ 10 parts of glucose, 1 ~ 3 part of bitter salt, 12 ~ 18 parts
Potassium dihydrogen phosphate, 0.03 ~ 0.06 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizing 20min.
Apple pectin：It is purchased from Shanghai Kai Yang bioengineering Co., Ltd.
A kind of preparation method of fish bait, includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 25 ~ 27 DEG C of activation cultures 10 ~ 12 days, obtains
Bacterium is activated, picking activation bacterium is seeded in strain fermentation culture medium by 3 ~ 5% inoculum concentration, cultivates 3 ~ 5 days, must send out in 27 ~ 33 DEG C
Yeast-like fungi liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 be added propane, in 25 ~ 35 DEG C of subcritical fluid extractions 30 ~
40min takes extract liquor to be evaporated under reduced pressure, and obtains vacuum distillation object, takes corn protein powder in 0.3MPa, 100 ~ 120s of steam blasting, obtains
Steam blasting object takes 3 ~ 7 parts of zymocyte liquids, 20 ~ 30 parts of vacuum distillation objects, 150 ~ 200 parts of steam blastings according to the mass fraction
Object, 2 ~ 5 parts of glucose, 0.3 ~ 0.8 part of bitter salt, 100 ~ 120 parts of water must be sent out in 25 ~ 28 DEG C of fermented and cultureds 18 ~ for 24 hours
Ferment culture, it is spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 30 ~ 40min in 50 ~ 55 DEG C, obtain stirring liquid, take
Stir liquid in mass ratio 1：5 are added the sodium hydroxide solution of 3 mol/L, are stirred 40 ~ 45min in 55 ~ 60 DEG C, are cooled to room
Temperature adjusts pH to 3 ~ 3.5, stands 10 ~ 12h, takes and stands liquid in mass ratio 1：2 ~ 5 are added then addition absolute ethyl alcohol, and stirring is mixed
2 ~ 3h is closed, filtering takes precipitation drying, obtains dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, 5 ~ 15 parts of fragrant fruits, 10 ~ 20 parts of asafoetides, 7 ~ 9 portions of cloves is taken to dry, pulverize 60 mesh sieve, sieving particle is collected, takes
Be sieved particle in mass ratio 1：50 are added distilled water, it is stirred 18 in 30 ~ 50 DEG C ~ for 24 hours, centrifugation takes supernatant in mass ratio
5：3：3 are added dried object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 200 ~ 300 parts of steps are taken（2）Spare fermentation culture medium, 50 ~ 70 parts of mixtures, 30 ~ 50 parts
Sodium alginate, 30 ~ 40 parts of Sodium Polyacrylates, 12 ~ 20 parts of glycine betaines, 60 ~ 80 parts of water mixing, are warming up to 55 ~ 60 DEG C and are stirred 2
~ 3h, obtains mixture, in 90r/min, 80 ~ 100 DEG C of extrusions, is granulated, dry, obtains fish bait.
Hickory chick：Me-01 is purchased from Jilin university wheat and corn deep processing laboratory.
Corn protein powder：The Jilin China Oil and Food Import and Export Corporation biochemistry energy sells Co., Ltd.
Strain fermentation culture medium：According to the mass fraction, 8 parts of glucose, 1 part of bitter salt, 12 parts of biphosphates are taken
Potassium, 0.03 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizing 20min.
Apple pectin：It is purchased from Shanghai Kai Yang bioengineering Co., Ltd.
A kind of preparation method of fish bait, includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 25 DEG C of activation cultures 10 days, bacterium must be activated,
Picking activation bacterium is seeded to by 3% inoculum concentration in strain fermentation culture medium, is cultivated 3 days in 27 DEG C, is obtained zymocyte liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 addition propane take extraction in 25 DEG C of subcritical fluid extraction 30min
It takes liquid to be evaporated under reduced pressure, obtains vacuum distillation object, take corn protein powder in 0.3MPa, steam blasting 100s, steam blasting object is obtained, by matter
Number meter is measured, 3 parts of zymocyte liquids, 20 parts of vacuum distillation objects, 150 parts of steam blasting objects, 2 parts of glucose, 0.3 part of seven hydration sulphur are taken
Sour magnesium, 100 parts of water obtain fermentation culture medium in 25 DEG C of fermented and cultured 18h, spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 30min in 50 DEG C, obtain stirring liquid, take stirring liquid
In mass ratio 1：5 be added 3 mol/L sodium hydroxide solution, be stirred 40min in 55 DEG C, be cooled to room temperature, adjust pH to
3,10h is stood, takes and stands liquid in mass ratio 1：2 are added then addition absolute ethyl alcohol, are stirred 2h, filter, take precipitation drying,
Obtain dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, 5 parts of fragrant fruits, 10 parts of asafoetides, 7 portions of cloves is taken to dry, pulverize 60 mesh sieve, sieving particle is collected, takes sieving particle
In mass ratio 1：50 are added distilled water, and 18h is stirred in 30 DEG C, centrifuge, take supernatant in mass ratio 5：3：3 are added drying
Object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 200 parts of steps are taken（2）Spare fermentation culture medium, 50 parts of mixtures, 30 parts of sodium alginates, 30
Part Sodium Polyacrylate, 12 parts of glycine betaines, 60 parts of water mixing, are warming up to 55 DEG C and are stirred 2h, obtain mixture, in 90r/min, 80
DEG C extrusion is granulated, dry, obtains fish bait.
Hickory chick：Me-01 is purchased from Jilin university wheat and corn deep processing laboratory.
Corn protein powder：The Jilin China Oil and Food Import and Export Corporation biochemistry energy sells Co., Ltd.
Strain fermentation culture medium：According to the mass fraction, 10 parts of glucose, 3 parts of bitter salts, 18 parts of biphosphates are taken
Potassium, 0.06 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizing 20min.
Apple pectin：It is purchased from Shanghai Kai Yang bioengineering Co., Ltd.
A kind of preparation method of fish bait, includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 27 DEG C of activation cultures 12 days, bacterium must be activated,
Picking activation bacterium is seeded to by 5% inoculum concentration in strain fermentation culture medium, is cultivated 5 days in 33 DEG C, is obtained zymocyte liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 addition propane take extraction in 35 DEG C of subcritical fluid extraction 40min
It takes liquid to be evaporated under reduced pressure, obtains vacuum distillation object, take corn protein powder in 0.3MPa, steam blasting 120s, steam blasting object is obtained, by matter
Number meter is measured, 7 parts of zymocyte liquids, 30 parts of vacuum distillation objects, 200 parts of steam blasting objects, 5 parts of glucose, 0.8 part of seven hydration sulphur are taken
Sour magnesium, 120 parts of water for 24 hours in 28 DEG C of fermented and cultureds obtain fermentation culture medium, spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 40min in 55 DEG C, obtain stirring liquid, take stirring liquid
In mass ratio 1：5 be added 3 mol/L sodium hydroxide solution, be stirred 45min in 60 DEG C, be cooled to room temperature, adjust pH to
3.5,12h is stood, takes and stands liquid in mass ratio 1：5 are added then addition absolute ethyl alcohol, are stirred 3h, filter, and take precipitation dry
It is dry, obtain dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, 15 parts of fragrant fruits, 20 parts of asafoetides, 9 portions of cloves is taken to dry, pulverize 60 mesh sieve, sieving particle is collected, takes sieving particle
In mass ratio 1：50 are added distilled water, are stirred in 50 DEG C for 24 hours, and centrifugation takes supernatant in mass ratio 5：3：3 are added drying
Object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 300 parts of steps are taken（2）Spare fermentation culture medium, 70 parts of mixtures, 50 parts of sodium alginates, 40
Part Sodium Polyacrylate, 20 parts of glycine betaines, 80 parts of water mixing, are warming up to 60 DEG C and are stirred 3h, obtain mixture, in 90r/min,
100 DEG C of extrusions are granulated, dry, obtain fish bait.
Hickory chick：Me-01 is purchased from Jilin university wheat and corn deep processing laboratory.
Corn protein powder：The Jilin China Oil and Food Import and Export Corporation biochemistry energy sells Co., Ltd.
Strain fermentation culture medium：According to the mass fraction, 9 parts of glucose, 2 parts of bitter salts, 15 parts of biphosphates are taken
Potassium, 0.04 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizing 20min.
Apple pectin：It is purchased from Shanghai Kai Yang bioengineering Co., Ltd.
A kind of preparation method of fish bait, includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 26 DEG C of activation cultures 11 days, bacterium must be activated,
Picking activation bacterium is seeded to by 4% inoculum concentration in strain fermentation culture medium, is cultivated 4 days in 30 DEG C, is obtained zymocyte liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 addition propane take extraction in 30 DEG C of subcritical fluid extraction 35min
It takes liquid to be evaporated under reduced pressure, obtains vacuum distillation object, take corn protein powder in 0.3MPa, steam blasting 110s, steam blasting object is obtained, by matter
Number meter is measured, 5 parts of zymocyte liquids, 25 parts of vacuum distillation objects, 175 parts of steam blasting objects, 3.5 parts of glucose, 0.55 part of seven water are taken
Magnesium sulfate is closed, 110 parts of water obtain fermentation culture medium in 27 DEG C of fermented and cultured 21h, spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 35min in 53 DEG C, obtain stirring liquid, take stirring liquid
In mass ratio 1：5 be added 3 mol/L sodium hydroxide solution, be stirred 43min in 57 DEG C, be cooled to room temperature, adjust pH to
3.3,11h is stood, takes and stands liquid in mass ratio 1：3 are added then addition absolute ethyl alcohol, are stirred 2.5h, filter, take precipitation
It is dry, obtain dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, 10 parts of fragrant fruits, 15 parts of asafoetides, 8 portions of cloves is taken to dry, pulverize 60 mesh sieve, sieving particle is collected, takes sieving particle
In mass ratio 1：50 are added distilled water, and 21h is stirred in 40 DEG C, centrifuge, take supernatant in mass ratio 5：3：3 are added drying
Object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 250 parts of steps are taken（2）Spare fermentation culture medium, 60 parts of mixtures, 40 parts of sodium alginates, 35
Part Sodium Polyacrylate, 16 parts of glycine betaines, 70 parts of water mixing, are warming up to 57 DEG C and are stirred 2.5h, obtain mixture, in 90r/min,
90 DEG C of extrusions are granulated, dry, obtain fish bait.
Comparative example：The fish bait of Yangzhou company production.
The fish bait that bait and comparative example are made according to the method described above is respectively fitted on fish hook and is used, its performance is tested,
Test result such as table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Taste | Raw meat fragrance | Raw meat fragrance | Raw meat fragrance | Tasteless |
| One hour number of ingesting | 25 | 23 | 23 | 13~14 |
| One hour number of rising to the bait | 22 | 21 | 21 | 9~10 |
In summary, fish bait palatability of the invention is strong, also can more significantly improve the catch rate of fish, has good attracting effect
Fruit.
Claims (2)
1. a kind of preparation method of fish bait, which is characterized in that the preparation method includes the following steps：
（1）It takes hickory chick to be seeded in PDA solid mediums by 2% inoculum concentration, in 25 ~ 27 DEG C of activation cultures 10 ~ 12 days, obtains
Bacterium is activated, picking activation bacterium is seeded to by 3 ~ 5% inoculum concentration in strain fermentation culture medium, in 27 ~ 33 DEG C of cultures, obtains zymophyte
Liquid；
（2）It takes fly maggot to be homogenized, takes homogenate in mass ratio 1:8 are added propane, and subcritical fluid extraction takes extract liquor decompression to steam
It evaporates, obtains vacuum distillation object, take corn protein powder steam blasting, obtain steam blasting object, according to the mass fraction, take 3 ~ 7 parts of zymophytes
Liquid, 20 ~ 30 parts of vacuum distillation objects, 150 ~ 200 parts of steam blasting objects, 2 ~ 5 parts of glucose, 0.3 ~ 0.8 part of bitter salt,
100 ~ 120 parts of water obtain fermentation culture medium in 25 ~ 28 DEG C of fermented and cultureds, spare；
（3）Take apple pectin in mass ratio 1：40 are added distilled water, are stirred 30 ~ 40min in 50 ~ 55 DEG C, obtain stirring liquid, take
Stir liquid in mass ratio 1：5 are added the sodium hydroxide solution of 3 mol/L, are stirred, are cooled to room temperature in 55 ~ 60 DEG C, adjust
PH to 3 ~ 3.5 is stood, and is taken and is stood liquid in mass ratio 1：2 ~ 5 are added then addition absolute ethyl alcohol, are stirred, filter, take precipitation
It is dry, obtain dried object；
（4）Take l-Alanine, DL- methionines in mass ratio 2：1：1 is added distilled water mixing, mixed amino acid solution is obtained, by matter
Number meter is measured, takes 5 ~ 15 portions of fragrant fruits, 10 ~ 20 parts of asafoetides, 7 ~ 9 portions of cloves to dry, pulverize sieving, collects sieving particle, take sieving
Particle in mass ratio 1：50 are added distilled water, are stirred in 30 ~ 50 DEG C, centrifuge, take supernatant in mass ratio 5：3：3 additions are dry
Dry object, the mixing of mixed amino acid solution, obtain mixture；
（5）According to the mass fraction, 200 ~ 300 parts of steps are taken（2）Spare fermentation culture medium, 50 ~ 70 parts of mixtures, 30 ~ 50 parts
Sodium alginate, 30 ~ 40 parts of Sodium Polyacrylates, 12 ~ 20 parts of glycine betaines, 60 ~ 80 parts of water mixing, are warming up to 55 ~ 60 DEG C and are stirred,
Mixture is obtained, extrusion is granulated, dry, obtains fish bait.
2. the preparation method of fish bait according to claim 1, which is characterized in that the step（1）Middle strain fermentation training
Support base be according to the mass fraction, take 8 ~ 10 parts of glucose, 1 ~ 3 part of bitter salt, 12 ~ 18 parts of potassium dihydrogen phosphates, 0.03 ~
0.06 part of green vitriol, 1000 parts of water, pH is naturally, 121 DEG C of sterilizing 20min.
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