CN108401868A - A kind of implantation methods of selenium-rich Asiatic plantain - Google Patents
A kind of implantation methods of selenium-rich Asiatic plantain Download PDFInfo
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- CN108401868A CN108401868A CN201810098824.5A CN201810098824A CN108401868A CN 108401868 A CN108401868 A CN 108401868A CN 201810098824 A CN201810098824 A CN 201810098824A CN 108401868 A CN108401868 A CN 108401868A
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- asiatic plantain
- implantation methods
- selenium
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- epiphysin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- Life Sciences & Earth Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The present invention provides a kind of implantation methods of selenium-rich Asiatic plantain, and this method comprises the following steps：Asiatic plantain to growing to 4 true leaf expansion carries out the epiphysin that leaf surface spraying concentration is 50~250 μm of ol/L.The present invention can be effectively promoted the growth of Asiatic plantain and improve Asiatic plantain well and the accumulation of selenium is enriched with, and solve the deficiency that special selenium fertilizer and planting patterns are needed existing for existing selenium-rich means, it is easier to actually promote the use of.
Description
Technical field
The invention belongs to Chinese herbal medicine planting technology fields, and in particular to a kind of implantation methods of selenium-rich Asiatic plantain.
Background technology
Asiatic plantain (Plantago asiatica L.) is a kind of traditional Chinese medicine, with seed and all herbal medicine, is had a variety of
Under important medical value, such as clearing heat and detoxicating, stranguria with turbid discharge band, oedema turgor, summer-heat and damp rush down dysentery, hot eyes are hidden from view, phlegm heat-syndrome cough is breathed heavily.Selenium is people
The component part of body erythrocyte glutathione peroxidase (GSH-Px), in body antioxidant system and protection biomembrane mechanism
In play a significant role.Xun Lihong etc. easily causes Kaschin-Beck disease, Keshan studies have shown that human internal environment is under low selenium state
A variety of diseases such as disease, psoriasis.The intake for improving selenium is to prevent the optimal method of selenium deficiency disease.China is from northeast to southwest
It is " poor selenium area " to have 45 areas, and most area Selenium In Foods content is less than 0.22mg/kg, exists and causes region selenium deficiency
The hidden danger of disease.Therefore solve the problems, such as that selenium deficiency has important science and practice significance.
Se-enriched herbal medicine is a kind of safely and effectively organic selenium-supply drug, compared to the inorganic seleniums drug such as sodium selenite piece,
With selenium-supply and health care double action.Zhang Xingguo etc. combines Chinese herbal medicine and selenium-rich area, tries hard to develop selenium-rich Chinese medicine
Have the function that prevention and health care, clearly proposes " the extraordinary medicinal material that selenium-rich Chinese medicine should be used as China is developed and used ".Li Jing
Deng the study found that the selenium rich ability between different plant differs greatly, and the Se content of same plant different parts also differs greatly,
It is an important directions of current research thus to improve the selenium rich ability of Chinese herbal medicine and regulate and control the selenium distribution of Different Organs.
Currently, the research in terms of crop selenium-rich is also relatively fewer, mainly carried out by applying selenium-enriched fertilizer, it is such as public
The number of opening be CN 104969816 A, CN 105210643 A and CN 106561374A Chinese patent.But, pass through fertilising
Mode carries out selenium-rich to crop, larger for the consumption of fertilizer；And since the selection of fertilizer plays the selenium-rich effect of crop
Decisive role, therefore in actual production, a small number of feasible planting patterns can only be used so that these technologies should not be promoted
It uses.If crop can be improved by relatively simple method to the absorbability of selenium, can solve at present in crop selenium-rich
The deficiency in field.But, considerably less in the research of the direction at present, and according to the inventors knowledge, in this direction in relation to Asiatic plantain
Research or blank.
Invention content
For the deficiency of prior art, the purpose of the present invention is to provide a kind of implantation methods of selenium-rich Asiatic plantain, the party
Method includes the following steps：
Asiatic plantain to growing to 4 true leaf expansion carries out the epiphysin that leaf surface spraying concentration is 50~250 μm of ol/L.
When carrying out the foliage-spray, dripped for degree with blade.
As a preferred solution of the present invention, a concentration of 200 μm of ol/L of the epiphysin.
It when carrying out the foliage-spray, sprays altogether twice, time interval is 7 days.
As an enforceable scheme of the invention, the growth substrate of Asiatic plantain is by perlite and vermiculite by volume 1:1
Composition.
When carrying out the foliage-spray, the positive and negative on blade face is carried out while being sprayed.
As the optional scheme of the present invention suitable Se-containing nutritive liquid is poured when carrying out Chinese herbaceous peony grass planting.
Since the present invention realizes raising of the Asiatic plantain for the accumulation ability of selenium, from preferably obtaining Se content
For the angle of higher Asiatic plantain, when planting Asiatic plantain, suitable selenium can be added into growth substrate.
Optionally, the Se-containing nutritive liquid is the Huo Gelan nutrient solutions that Se content is 10mg/L.The Se content is 10mg/
The irrigation amount of the Huo Gelan nutrient solutions of L, which is subject to, irrigates growth substrate.In this scenario, the Se content is the Huo Ge of 10mg/L
The pouring period of blue nutrient solution is preferably 2 days.
Beneficial effects of the present invention：
The present invention can be effectively promoted the growth of Asiatic plantain and improve Asiatic plantain well and the accumulation of selenium is enriched with, solution
It has determined and has needed the deficiency of special selenium fertilizer and planting patterns existing for existing selenium-rich means, it is easier to actually promote the use of.
Specific implementation mode
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
1 materials and methods
1.1 material
Asiatic plantain seedling picks up from Sichuan Province Wenjiang district of Chengdu city farmland in October, 2016.
1.2 test method
It tests and was carried out in Sichuan Agricultural University Chengdu school district in -2017 years 2016.In October, 2016, by a diameter of 20cm
Plastic flowerpot fill matrix (by 1:The perlite and vermiculite of 1 mixing), it is irrigated with ionized water, then true by consistent 4 are grown
The Asiatic plantain of leaf expansion is transplanted into basin, and the Huo Gelan nutrient solutions that a Se content is 10mg/L are poured in uniformly 4 plants of the plantation per basin
20ml, and the Huo Gelan nutrient solutions that 1 Se content is 10mg/L were poured every 2 days, each 30ml (nutrient solution irrigates subject to matrix),
Until harvest.Asiatic plantain is transplanted after a week, and the uniform spraying of epiphysin of 0,50,100,150,200 and 250 μm of ol/L concentration is existed
The tow sides of leaf, spray 10ml (being dripped for degree with blade) per basin, each processing is repeated 5 times, and basin spacing is 10cm, completely random
It puts；It sprays 1 epiphysin again after a week, sprays 2 times altogether.Measure the photosynthetic of Asiatic plantain blade in December, 2016 (60d after transplanting)
Pigment content and antioxidase (SOD, POD and CAT) activity.Then whole strain harvest, by root system of plant and aerial part plant point
It does not encapsulate, is cleaned with tap water, then rinsed 3 times with deionized water, finish 15min in 110 DEG C, and 75 DEG C are dried to weight, weigh,
It crushes, sieves with 100 mesh sieve.0.500g plant samples are weighed, disappears and is contained with atomic fluorescence spectrophotometer (AFS-810) measurement selenium after boiling
Amount.
1.3 data processing
Variance analysis (Duncan duncan's new multiple range methods carry out Multiple range test) is carried out using SPSS data processing softwares, with average
Value ± standard deviation indicates.Resistance factor=processing group total biomass/control group total biomass.
Concentration coefficient (BCF)=root system (or above-ground plant parts) Se content/soil Se content
Transport coefficient (TF)=above-ground plant parts Se content/root system Se content
2 results and analysis
Influence of the 2.1 different epiphysin concentration to Asiatic plantain biomass
As it can be seen from table 1 with the raising of epiphysin concentration, Asiatic plantain root system, aerial part and whole strain biomass are equal
In first increasing the trend reduced afterwards.In a concentration of 100,150,200 and 250 μm of ol/L of epiphysin, each position biology of Asiatic plantain
Amount is all remarkably higher than control (P ＜ 0.05).In a concentration of 200 μm of ol/L of epiphysin, Asiatic plantain root system, aerial part and whole strain
Biomass reaches maximum, improves 15.50%, 14.29% and 14.89% compared with control respectively.The Asiatic plantain of epiphysin processing
Root/shoot ratio is above control, and as a concentration of 100 μm of ol/L of epiphysin, Asiatic plantain root/shoot ratio is 0.986, reaches maximum.With taking off
The raising of melanocyte concentration, Asiatic plantain resistance factor is in first increasing the trend reduced afterwards, and in a concentration of 200 μm of ol/L of epiphysin
Reach highest, illustrates that the epiphysin of suitable concentration can effectively improve the resistance of Asiatic plantain, contribute to the growth of Asiatic plantain.
Influence of the different epiphysin concentration of table 1 to Asiatic plantain biomass
Note：Data are mean+SD；English lower case difference indicates certain between different disposal after same column data
Indicator difference significantly (P<0.05), similarly hereinafter.
Influence of the 2.2 different epiphysin concentration to Asiatic plantain Contents of Photosynthetic Pigments
From table 2 it can be seen that the epiphysin processing of various concentration significantly improve the chlorophyll a of Asiatic plantain, chlorophyll b,
Carotenoid and chlorophyll total amount content.With the raising of epiphysin concentration, each Contents of Photosynthetic Pigments of Asiatic plantain is in first to increase
The trend reduced afterwards.In a concentration of 200 μm of ol/L of epiphysin, Asiatic plantain chlorophyll a, chlorophyll b, carotenoid and leaf are green
Plain total amount content reaches maximum value, improves 39.25%, 36.95%, 44.02% and 38.71% compared with control respectively, explanation
Epiphysin can effectively enhance the photosynthesis of Asiatic plantain.For chlorophyll a/b, in a concentration of 50 and 250 μm of ol/ of epiphysin
When L, Asiatic plantain chlorophyll a/b decreases compared with control, and other processing improve Asiatic plantain chlorophyll a/b.
Influence of the different epiphysin concentration of table 2 to Asiatic plantain Contents of Photosynthetic Pigments
Influence of the 2.3 different epiphysin concentration to Asiatic plantain enzymatic activity
As known from Table 3, with the increase of epiphysin concentration, Asiatic plantain soluble protein content, SOD activity, POD activity and
CAT activity is in first to increase the trend reduced afterwards.Each epiphysin concentration handles the Asiatic plantain soluble protein that has been significantly increased and contains
Amount and CAT activity, as a concentration of 200 μm of ol/L of epiphysin, Asiatic plantain soluble protein content, SOD activity, POD activity and
CAT activity reaches maximum value, improves 236.56%, 16.98%, 47.90% and 122.16% compared with control respectively.Thus it says
Bright epiphysin can improve the activities of antioxidant enzymes of Asiatic plantain.
Influence of the different epiphysin concentration of table 3 to Asiatic plantain activities of antioxidant enzymes
Influence of the 2.4 different epiphysin concentration to Asiatic plantain Se content
From table 4, it can be seen that with the increase of epiphysin concentration, the Se content of Asiatic plantain root system and aerial part is in first
The trend reduced after raising, and the processing of various concentration epiphysin reaches notable water to the raising of the Se content of Asiatic plantain root system
It is flat.In a concentration of 200 μm of ol/L of epiphysin, the Se content of Asiatic plantain root system and aerial part reaches maximum value, respectively compared with
Control improves 60.45% and 23.01%.For concentration coefficient, with the increase of epiphysin concentration, the enrichment of Asiatic plantain root system
Coefficient and aerial part concentration coefficient are in first to increase the trend reduced afterwards.In a concentration of 200 μm of ol/L of epiphysin, Asiatic plantain
Root system concentration coefficient and aerial part concentration coefficient respectively reach maximum value, are 43.33 and 13.60, show taking off for 200 μm of ol/L
Melanocyte concentration keeps the selenium-rich effect of Asiatic plantain best.In addition, epiphysin reduces the transhipment coefficient of Asiatic plantain, illustrate that epiphysin presses down
The ability that selenium is transported to aerial part by car fare grass under ground portion is made.
Influence of the different epiphysin concentration of table 4 to Asiatic plantain Se content
It is a discovery of the invention that after epiphysin is handled, Se content, root system and the aerial part of Asiatic plantain root system and aerial part
Concentration coefficient is improved compared with control, it may be possible to because epiphysin enhances patience of the Asiatic plantain to Selenium in Soil, and then improve
Ability of the Asiatic plantain to Se accumulation.But, the mechanism of the influence in relation to epiphysin to Asiatic plantain Se content need further
Research.
Claims (10)
1. a kind of implantation methods of selenium-rich Asiatic plantain, which is characterized in that described method includes following steps：
Asiatic plantain to growing to 4 true leaf expansion carries out the epiphysin that leaf surface spraying concentration is 50~250 μm of ol/L.
2. implantation methods according to claim 1, which is characterized in that when carrying out the foliage-spray, with blade drip for
Degree.
3. implantation methods according to claim 1, which is characterized in that a concentration of 200 μm of ol/L of the epiphysin.
4. implantation methods according to claim 1, which is characterized in that when carrying out the foliage-spray, spray altogether twice, when
Between between be divided into 7 days.
5. implantation methods according to claim 1, which is characterized in that the growth substrate of Asiatic plantain is pressed by perlite and vermiculite
Volume ratio 1:1 composition.
6. implantation methods according to claim 1, which is characterized in that when carrying out the foliage-spray, to the positive and negative of blade face
Face carries out while spraying.
7. implantation methods according to claim 1, which is characterized in that when carrying out Chinese herbaceous peony grass planting, pour suitable containing selenium battalion
Nutrient solution.
8. implantation methods according to claim 1, which is characterized in that it is 10mg/L's that the Se-containing nutritive liquid, which is Se content,
Huo Gelan nutrient solutions.
9. implantation methods according to claim 8, which is characterized in that the Se content is the Huo Gelan nutrient solutions of 10mg/L
Irrigation amount be subject to and irrigate growth substrate.
10. implantation methods according to claim 9, which is characterized in that the Se content is the Huo Gelan nutrition of 10mg/L
The pouring period of liquid is 2 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810098824.5A CN108401868A (en) | 2018-01-31 | 2018-01-31 | A kind of implantation methods of selenium-rich Asiatic plantain |
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| CN201810098824.5A CN108401868A (en) | 2018-01-31 | 2018-01-31 | A kind of implantation methods of selenium-rich Asiatic plantain |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900989A (en) * | 2016-04-18 | 2016-08-31 | 浙江大学 | Application of melatonin in improvement of absorption of sulfur by vegetables |
| CN106576768A (en) * | 2016-11-26 | 2017-04-26 | 全椒县悠然自得果蔬种植专业合作社 | Planting method for improving superior product rate of melons |
| CN106831183A (en) * | 2017-03-15 | 2017-06-13 | 湖北工程学院 | Selenium-rich auxin, foliar fertilizer and preparation method thereof, selenium-rich rapeseed cultivation method |
| CN106922359A (en) * | 2017-03-20 | 2017-07-07 | 明光现代农业科技合作推广服务中心 | A kind of method that utilization external source epiphysin improves stevia rebaudianum sugared content |
-
2018
- 2018-01-31 CN CN201810098824.5A patent/CN108401868A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900989A (en) * | 2016-04-18 | 2016-08-31 | 浙江大学 | Application of melatonin in improvement of absorption of sulfur by vegetables |
| CN106576768A (en) * | 2016-11-26 | 2017-04-26 | 全椒县悠然自得果蔬种植专业合作社 | Planting method for improving superior product rate of melons |
| CN106831183A (en) * | 2017-03-15 | 2017-06-13 | 湖北工程学院 | Selenium-rich auxin, foliar fertilizer and preparation method thereof, selenium-rich rapeseed cultivation method |
| CN106922359A (en) * | 2017-03-20 | 2017-07-07 | 明光现代农业科技合作推广服务中心 | A kind of method that utilization external source epiphysin improves stevia rebaudianum sugared content |
Non-Patent Citations (1)
| Title |
|---|
| MENGQI LI等: "Melatonin mediates selenium-induced tolerance to cadmium stress in tomato plants", 《JOURNAL OF PINEAL RESEARCH》 * |
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