CN108379317A - The preparation method of ter penoids in a kind of day name essence - Google Patents
The preparation method of ter penoids in a kind of day name essence Download PDFInfo
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- CN108379317A CN108379317A CN201810221916.8A CN201810221916A CN108379317A CN 108379317 A CN108379317 A CN 108379317A CN 201810221916 A CN201810221916 A CN 201810221916A CN 108379317 A CN108379317 A CN 108379317A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses the preparation methods of ter penoids in a kind of day name essence, and steps are as follows：Name keen-witted and capable dry herb in day is crushed, is extracted 24 times with 85 95% alcohol reflux of volume fraction, 2 3h, merges extracting solution every time, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract to use petroleum ether, ethyl acetate and extracting n-butyl alcohol 35 times after appropriate water dissolution successively, extract liquor is recovered under reduced pressure, and ethyl acetate medicinal extract is taken to be detached through silica gel column chromatography, uses volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform methanol elution, collects 25:1 elution fraction, concentrates to obtain the final product.The preparation method is easy to operate, and obtained extract is rich in terpenoid, has antitumor, anti-inflammatory isoreactivity, can be prepared applied to drug as the active ingredient of day name essence.
Description
Technical field
The invention belongs to field of medicaments, and in particular to the preparation method of ter penoids in a kind of day name essence.
Background technology
Its name essenceCarpesium abrotanoidesL. it is composite family（Compositae）Carpesium CarpesiumL.
Herbaceos perennial also known as day it is graceful it is green, ancient name for Chinese cabbage；It is distributed in China East China, south China, Central China, each provinces and regions in southwest and Korea, day
Sheet, Vietnam, Burma, Sillim, Iran and Russia；Be born in by village, roadside wasteland, small stream side and border, vertical distribution is up to height above sea level
2000m.Its name essence herb is pharmaceutically acceptable, has effects that clearing heat and detoxicating, removing blood stasis and analgesics, hemostasis and desinsection, for treating Lungs of Children
The diseases such as inflammation, pain in chest and hypochondrium, malaria, dysentery, traumatic injury, venomous snake bite.Its seed is " Lappula echinata ", is in Chinese medicine desinsection side
Important drugs cure mainly roundworm disease, enterobiasis, taeniasis, abdominal pain due to enterositosis.Meanwhile as ethnic drug famous simply, band root is complete
Grass has extensive use in the ethnic groups such as Miao ethnic group, Bai nationality, Tujia.Phytochemistry and pharmaceutical research show day name essence
Rich in terpenoids such as sequiterpene, monoterpenes, with antitumor, anti-inflammatory and anti parasitic isoreactivity.
Invention content
The purpose of the present invention is to provide the preparation methods of ter penoids in a kind of day name essence.
The present invention is realized by following technical solution：
The preparation method of ter penoids, includes the following steps in a kind of day name essence：Name keen-witted and capable dry herb in day is crushed, with volume point
Number 85-95% alcohol refluxs extract 2-4 times, each 2-3h, merge extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with
Petroleum ether, ethyl acetate and extracting n-butyl alcohol 3-5 times are used after appropriate water dissolution successively, extract liquor is recovered under reduced pressure, and takes ethyl acetate
Medicinal extract is detached through silica gel column chromatography, uses volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform-methanol elution, is collected
25:1 elution fraction, concentrates to obtain the final product.
Preferably, it in the preparation method, is extracted 3 times with 90% alcohol reflux of volume fraction.
Preferably, in the preparation method, 2.5h is extracted every time.
Preferably, in the preparation method, 1kg days name essences carry out refluxing extraction using 9-11L solvents.
Preferably, in the preparation method, the 5-7mL water dissolutions of 1g medicinal extract.
The technology of the present invention effect：
The preparation method is easy to operate, and obtained extract is rich in terpenoid, has antitumor, anti-inflammatory isoreactivity, can be with
Active ingredient as day name essence is prepared applied to drug.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
The preparation method of ter penoids, includes the following steps in a kind of day name essence：Name keen-witted and capable dry herb in day is crushed, with volume point
Several 90% alcohol refluxs extract 3 times, each 2.5h, merge extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with appropriate
Petroleum ether, ethyl acetate and extracting n-butyl alcohol 4 times are used after water dissolution successively, extract liquor is recovered under reduced pressure, and ethyl acetate medicinal extract is taken to pass through
Silica gel column chromatography detaches, and uses volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform-methanol elution, collects 25:1 washes
De- part, concentrates to obtain the final product.
Wherein, 1kg days name essences carry out circumfluence distillation, the 6mL water dissolutions of 1g medicinal extract using 10L solvents.
Embodiment 2
The preparation method of ter penoids, includes the following steps in a kind of day name essence：Name keen-witted and capable dry herb in day is crushed, with volume point
Several 85% alcohol refluxs extract 2 times, each 2h, merge extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with suitable quantity of water
Petroleum ether, ethyl acetate and extracting n-butyl alcohol 3 times are used after dissolving successively, extract liquor is recovered under reduced pressure, and takes ethyl acetate medicinal extract through silicon
Rubber column gel column chromatographic isolation uses volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform-methanol elution, collects 25:1 elution
Part concentrates to obtain the final product.
Wherein, 1kg days name essences carry out circumfluence distillation, the 5mL water dissolutions of 1g medicinal extract using 9L solvents.
Embodiment 3
The preparation method of ter penoids, includes the following steps in a kind of day name essence：Name keen-witted and capable dry herb in day is crushed, with volume point
Several 95% alcohol refluxs extract 4 times, each 3h, merge extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with suitable quantity of water
Petroleum ether, ethyl acetate and extracting n-butyl alcohol 5 times are used after dissolving successively, extract liquor is recovered under reduced pressure, and takes ethyl acetate medicinal extract through silicon
Rubber column gel column chromatographic isolation uses volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform-methanol elution, collects 25:1 elution
Part concentrates to obtain the final product.
Wherein, 1kg days name essences carry out circumfluence distillation, the 7mL water dissolutions of 1g medicinal extract using 11L solvents.
The preparation method is easy to operate, and obtained extract is rich in terpenoid, has antitumor, anti-inflammatory isoreactivity,
It can be prepared applied to drug as the active ingredient of day name essence.
Claims (5)
1. the preparation method of ter penoids in a kind of day name essence, which is characterized in that include the following steps：By the keen-witted and capable dry herb of day name
It crushes, is extracted 2-4 times, each 2-3h with volume fraction 85-95% alcohol refluxs, merge extracting solution, extracting solution is concentrated under reduced pressure to give
Medicinal extract；By medicinal extract to use petroleum ether, ethyl acetate and extracting n-butyl alcohol 3-5 times after appropriate water dissolution successively, extract liquor depressurizes back
It receives, ethyl acetate medicinal extract is taken to be detached through silica gel column chromatography, use volume ratio 45 successively:1、35:1、25:1、15:1、5:1 chloroform-
Methanol elutes, and collects 25:1 elution fraction, concentrates to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：It is extracted 3 times with 90% alcohol reflux of volume fraction.
3. preparation method according to claim 2, it is characterised in that：Extraction 2.5h every time.
4. preparation method according to claim 1, it is characterised in that：1kg days name essences carry out reflux using 9-11L solvents and carry
It takes.
5. preparation method according to claim 1, it is characterised in that：The 5-7mL water dissolutions of 1g medicinal extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810221916.8A CN108379317A (en) | 2018-03-18 | 2018-03-18 | The preparation method of ter penoids in a kind of day name essence |
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| CN201810221916.8A CN108379317A (en) | 2018-03-18 | 2018-03-18 | The preparation method of ter penoids in a kind of day name essence |
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| CN108379317A true CN108379317A (en) | 2018-08-10 |
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| CN201810221916.8A Withdrawn CN108379317A (en) | 2018-03-18 | 2018-03-18 | The preparation method of ter penoids in a kind of day name essence |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112402412A (en) * | 2020-11-18 | 2021-02-26 | 中国医学科学院药用植物研究所 | Application of inner ester compound of jingdao in preparing medicine for treating inflammation-caused diseases |
| CN114569643A (en) * | 2022-03-30 | 2022-06-03 | 山东视泰明药业有限公司 | Application of carpesium abrotanoides in treatment of early meibomian cyst |
-
2018
- 2018-03-18 CN CN201810221916.8A patent/CN108379317A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112402412A (en) * | 2020-11-18 | 2021-02-26 | 中国医学科学院药用植物研究所 | Application of inner ester compound of jingdao in preparing medicine for treating inflammation-caused diseases |
| CN112402412B (en) * | 2020-11-18 | 2022-03-18 | 中国医学科学院药用植物研究所 | Application of inner ester compound of jingdao in preparing medicine for treating inflammation-caused diseases |
| CN114569643A (en) * | 2022-03-30 | 2022-06-03 | 山东视泰明药业有限公司 | Application of carpesium abrotanoides in treatment of early meibomian cyst |
| CN114569643B (en) * | 2022-03-30 | 2023-03-10 | 山东视泰明药业有限公司 | Application of carpesium abrotanoides in treating early-stage chalazion |
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Application publication date: 20180810 |