CN102362949B - Medicine for treating chronic pelvic inflammation and preparation method thereof - Google Patents
Medicine for treating chronic pelvic inflammation and preparation method thereof Download PDFInfo
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- CN102362949B CN102362949B CN201110107353.8A CN201110107353A CN102362949B CN 102362949 B CN102362949 B CN 102362949B CN 201110107353 A CN201110107353 A CN 201110107353A CN 102362949 B CN102362949 B CN 102362949B
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Abstract
The invention which belongs to the technical field of medicines concretely discloses a medicine for treating chronic pelvic inflammation and a preparation method thereof. The medicine of the invention comprises the following raw materials, by weight, 16.5-33.0 parts of an effective extract of Smilax bockii Warb, 2.19-8.76 parts of an effective extract of Radix Astragali and 0.65-2.6 parts of an effective extract of Rhizoma Corydalis, wherein the effective extract of Smilax bockii Warb comprises total flavonoids and total saponins of Smilax bockii Warb, the effective extract of Radix Astragali is Astragaloside IV, and the effective extract of Rhizoma Corydalis comprises total alkaloids of Rhizoma Corydalis. The preparation of the effective extract of Smilax bockii Warb, the preparation of the effective extract of Radix Astragali, the preparation of the effective extract of Rhizoma Corydalis, and the mixing of the three effective extracts are carried out in the invention to prepare a tablet, a granule, a pill or a capsule. Smilax bockii Warb, Radix Astragali and Rhizoma Corydalis which are selected in the invention have obvious antiinflammatory effects, and the three medicines show a synergistic effect, so the absorption effect is better after purifying through the method of the invention, thereby the treatment effect of the medicine of the invention is better.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of medicine for the treatment of chronic pelvic inflammatory disease and preparation method thereof.
Background technology
Chronic pelvic inflammatory disease is common gynecological disease, in many ways sick, common pelvic inflammatory disease fail thoroughly treatment or patient's body constitution poor, due to the passing of time disease prolongs.The primary disease state of an illness is obstinate, and more, even impact fertility, brings very large misery to patient to touching difficulty.Because the chronic pelvic inflammatory disease cause of disease is complicated, diseased region is special, pathogenic bacteria produces drug resistance feature, Western medicine aspect treatment, show weak curative effect or unstable, side effect large, long-term taking can cause cumulative action and produce its complication.And existing Chinese herbal medicine, although the aspects such as its treatment inflammation absorption, healing of focus and prevention are better than Western medicine, its course for the treatment of is long, medicine suffering clothes, and patient is often difficult to adhere to and affects the treatment.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide chronic medicine of a kind of better efficacy and preparation method thereof.
For achieving the above object, technical scheme that the present invention adopts is as follows:
A medicine for the treatment of chronic pelvic inflammatory disease, is characterized in that, its raw material weight umber ratio is:
16.5～33.0 parts of Rhizoma Smilacis Bockii effective sites, 2.19～8.76 parts of Radix Astragali effective sites, 0.65～2.6 part of Rhizoma Corydalis effective site; Described Rhizoma Smilacis Bockii effective site is Rhizoma Smilacis Bockii total flavones and total saponins, and described Radix Astragali effective site is astragaloside, and described Rhizoma Corydalis effective site is Rhizoma Corydalis total alkaloids.
Preferably, in the medicine of described treatment chronic pelvic inflammatory disease, its raw material weight umber ratio is:
24.75 parts of Rhizoma Smilacis Bockii effective sites, 4.38 parts of Radix Astragali effective sites, 1.3 parts of Rhizoma Corydalis effective sites.
A preparation method for described chronic pelvic inflammatory disease medicine, comprises the step of producing and above-mentioned three kinds of effective sites being mixed and made into tablet, granule, pill or capsule of the producing of the producing of Rhizoma Smilacis Bockii effective site, Radix Astragali effective site, Rhizoma Corydalis effective site;
Described Rhizoma Smilacis Bockii effective site, Radix Astragali effective site and Rhizoma Corydalis effective site to produce step as follows:
By Rhizoma Smilacis Bockii, the Radix Astragali or Rhizoma Corydalis after pulverizing, by alcohol-water solution, carry out reflux, extract, and filter, obtain filtrate;
Described filtrate is concentrated and remove alcohol, obtain concentrated solution;
By after the capable polyamide column of described concentrated solution or macroporous resin column or gel chromatographic columns, with alcohol-water solution eluting, collect to obtain eluent;
Described eluent concentrate drying is obtained to its effective site.
Preferably, in the preparation method of described chronic pelvic inflammatory disease medicine, described Rhizoma Smilacis Bockii effective site produce step, specifically:
Get Rhizoma Smilacis Bockii pulverizing medicinal materials and become coarse powder, adopt 60% alcohol reflux twice, add for the first time 12 times of amount solvent extraction 2h, add for the second time 10 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Rhizoma Smilacis Bockii medical material 5/9 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 in the speed of 2BV/h, the post bed of resin obtains diameter and is 1: 6 at high proportion, with distilled water, with the flow velocity eluting 6BV of 1.5-2BV/h, to eluent, become colourless, use again the flow velocity drip washing 6BV of 20% ethanol 1.5-2BV/h, discard, flow velocity eluting 5BV with 70% ethanol 1.5-2BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is drying to obtain Rhizoma Smilacis Bockii effective site again, yield must not be less than 1.0%.
Preferably, in the preparation method of described chronic pelvic inflammatory disease medicine, described Radix Astragali effective site produce step, specifically:
Get Milkvetch Root decoction pieces, adopt water reflux, extract, twice, add for the first time 10 times of amount solvent extraction 2h, add for the second time 8 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Milkvetch Root 5/8 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 1BV/h, and the post bed of resin obtains diameter and is 1: 6 at high proportion, washing 3BV, flow velocity 2BV/h, with 70% ethanol elution 3BV, flow velocity 2BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-170 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Radix Astragali effective site, yield must not be less than 1.0%.
Preferably, in the preparation method of described chronic pelvic inflammatory disease medicine, described Rhizoma Corydalis effective site produce step, specifically:
Get corydalis tuber medicinal material and be ground into coarse powder, adopt 60% alcohol reflux twice, add for the first time 6 times of amount solvent extraction 2h, add for the second time 4 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the concentrated solution that concentration is 0.4g/ml;
The heavy dried resin of corydalis tuber medicinal material 1/4 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 2BV/h, the post bed of resin obtains diameter and is 1: 6 at high proportion, with distilled water eluting 6BV, flow velocity is 2-3BV/h, then discards with 10% ethanol drip washing 2BV, and flow velocity is 2-3BV/h, 70% ethanol elution 5BV, flow velocity is 2-3BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 0.90-1.05, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Rhizoma Corydalis effective site, yield must not be less than 0.03%.
The Rhizoma Smilacis Bockii that the present invention selects, Rhizoma Corydalis and the Radix Astragali all have obvious antiinflammatory action, in addition three kinds of medicines show synergism each other, and by making its assimilation effect better after the inventive method purification, and then make the therapeutic effect of medicine of the present invention better.
The specific embodiment
With specific implementation method, describe the present invention in detail below, in exemplary embodiment and description of the present invention, be used for explaining the present invention, but not as a limitation of the invention.
Embodiment 1:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the capsule thereof of pelvic inflammatory disease.
Wherein, its raw material weight of this medicine is than as follows:
24.75 grams of Rhizoma Smilacis Bockii effective sites, 4.38 grams of Radix Astragali effective sites, 1.3 grams of Rhizoma Corydalis effective sites.
The preparation method of its capsule, comprises the steps:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 900g, and Rhizoma Corydalis 200g, Radix Astragali 200g is standby;
(2) producing of Rhizoma Smilacis Bockii effective site:
Get Rhizoma Smilacis Bockii pulverizing medicinal materials and become coarse powder, adopt 60% alcohol reflux twice, add for the first time 12 times of amount solvent extraction 2h, add for the second time 10 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Rhizoma Smilacis Bockii medical material 5/9 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 in the speed of 2BV/h, the post bed of resin obtains diameter and is 1: 6 at high proportion, with distilled water, with the flow velocity eluting 6BV of 1.5-2BV/h, to eluent, become colourless, use again the flow velocity drip washing 6BV of 20% ethanol 1.5-2BV/h, discard, flow velocity eluting 5BV with 70% ethanol 1.5-2BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is drying to obtain Rhizoma Smilacis Bockii effective site again, yield must not be less than 1.0%.
(3) producing of Radix Astragali effective site:
Get Milkvetch Root decoction pieces, adopt water reflux, extract, twice, add for the first time 10 times of amount solvent extraction 2h, add for the second time 8 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Milkvetch Root 5/8 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 1BV/h, and the post bed of resin obtains diameter and is 1: 6 at high proportion, washing 3BV, flow velocity 2BV/h, with 70% ethanol elution 3BV, flow velocity 2BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-170 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Radix Astragali effective site, yield must not be less than 1.0%.
(4) producing of Rhizoma Corydalis effective site:
Get corydalis tuber medicinal material and be ground into coarse powder, adopt 60% alcohol reflux twice, add for the first time 6 times of amount solvent extraction 2h, add for the second time 4 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the concentrated solution that concentration is 0.4g/ml;
The heavy dried resin of corydalis tuber medicinal material 1/4 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 2BV/h, the post bed of resin obtains diameter and is 1: 6 at high proportion, with distilled water eluting 6BV, flow velocity is 2-3BV/h, then discards with 10% ethanol drip washing 2BV, and flow velocity is 2-3BV/h, 70% ethanol elution 5BV, flow velocity is 2-3BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 0.90-1.05, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Rhizoma Corydalis effective site, yield must not be less than 0.03%.
(5) dry powder of step (2), (3), (4) is merged, mix, add appropriate amount of starch, dextrin, microcrystalline Cellulose and carboxymethyl starch sodium and magnesium stearate, make 1000 of finished products, every 0.4g, packs and get final product.
It should be noted that, wherein ethanol used can also be less than 32 alkanols by methanol, propanol, butanols, ethylene glycol, carbochain, carbochain is less than the replacements such as 32 enols and phenol, and these are all protection scope of the present invention.
It should be noted that, wherein big pore resin comprises the domestic or import macroporous adsorbent resin of nonpolar, low pole, Semi-polarity, polarity, and these are all protection scope of the present invention.
Embodiment 2:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the granule thereof of pelvic inflammatory disease.Being from the different of embodiment 1 of this enforcement: its material rate, with and preparation method thereof (1) with (5) step.
Wherein, its raw material weight is than as follows:
16.5 grams of Rhizoma Smilacis Bockii effective sites, 2.19 grams of Radix Astragali effective sites, 0.65 gram of Rhizoma Corydalis effective site.
Wherein, (1) and (5) step of its process for producing granula, specific as follows:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 600g, and Rhizoma Corydalis 100g, Radix Astragali 100g is standby;
(5) dry powder of step (2), (3), (4) is merged, mix, add appropriate amount of starch and Icing Sugar, the appropriate alcoholic solution of spray people, granulates, dry, dress pouch, and every bag of 10g, gets product 300 bags.
Embodiment 3:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the tablet thereof of pelvic inflammatory disease.Being from the different of embodiment 1 of this enforcement: its material rate, with and preparation method thereof (1) with (5) step.
Wherein, its raw material weight is than as follows:
33 grams of Rhizoma Smilacis Bockii effective sites, 8.76 grams of Radix Astragali effective sites, 2.6 grams of Rhizoma Corydalis effective sites.
Wherein, (1) and (5) step of its method for preparing tablet thereof, specific as follows:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 1200g, and Rhizoma Corydalis 400g, Radix Astragali 400g is standby;
(5) the effective site dry powder of step (2), (3), (4) is merged, mix, add appropriate conventional pharmaceutic adjuvant, granulate, tabletting has just been prepared into the tablet of medicine of the present invention, and every heavy 0.4g, makes 1250 of finished products, packs and get final product.
Embodiment 4:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the tablet thereof of pelvic inflammatory disease.Being from the different of embodiment 1 of this enforcement: its material rate, with and preparation method thereof (1) with (5) step.
Wherein, its raw material weight is than as follows:
19.25 grams of Rhizoma Smilacis Bockii effective sites, 3.285 grams of Radix Astragali effective sites, 0.975 gram of Rhizoma Corydalis effective site.
Wherein, (1) and (5) step of its method for preparing tablet thereof, specific as follows:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 700g, Rhizoma Corydalis 150g, Radix Astragali 150g;
(5) the effective site dry powder of step (2), (3), (4) is merged, mix, add appropriate conventional pharmaceutic adjuvant, granulate, tabletting has just been prepared into the tablet of medicine of the present invention, and every heavy 0.4g, makes 1250 of finished products, packs and get final product.
Embodiment 5:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the tablet thereof of pelvic inflammatory disease.Being from the different of embodiment 1 of this enforcement: its material rate, with and preparation method thereof (1) with (5) step.
Wherein, its raw material weight is than as follows:
27.5 grams of Rhizoma Smilacis Bockii effective sites, 5.475 grams of Radix Astragali effective sites, 1.625 grams of Rhizoma Corydalis effective sites.
Wherein, (1) and (5) step of its method for preparing tablet thereof, specific as follows:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 1000g, Rhizoma Corydalis 250g, Radix Astragali 250g;
(5) the effective site dry powder of step (2), (3), (4) is merged, mix, add appropriate conventional pharmaceutic adjuvant, granulate, tabletting has just been prepared into the tablet of medicine of the present invention, and every heavy 0.4g, makes 1250 of finished products, packs and get final product.
Embodiment 6:
The present embodiment discloses a kind of preparation method for the treatment of medicine and the tablet thereof of pelvic inflammatory disease.Being from the different of embodiment 1 of this enforcement: its material rate, with and preparation method thereof (1) with (5) step.
Wherein, its raw material weight is than as follows:
19.25 grams of Rhizoma Smilacis Bockii effective sites, 5.475 grams of Radix Astragali effective sites, 0.975 gram of Rhizoma Corydalis effective site.
Wherein, (1) and (5) step of its method for preparing tablet thereof, specific as follows:
(1) according to the average receipts cream rate after Rhizoma Smilacis Bockii, Rhizoma Corydalis and Radix Astragali effective site purification, be respectively 2.75%, 0.65% and 2.19%, correspondence takes each crude drug: Rhizoma Smilacis Bockii 700g, Rhizoma Corydalis 150g, Radix Astragali 250g;
(5) the effective site dry powder of step (2), (3), (4) is merged, mix, add appropriate conventional pharmaceutic adjuvant, granulate, tabletting has just been prepared into the tablet of medicine of the present invention, and every heavy 0.4g, makes 1250 of finished products, packs and get final product.
Below by the present invention, the impact of the swollen model of rat granuloma is further illustrated to the present invention:
1, tested material
Compositions 1: for the compositions of embodiment 1 preparation, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 9: 2: 2.
Compositions 2: for the compositions of embodiment 2 preparations, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 6: 1: 1.
Compositions 3: for the compositions of embodiment 3 preparations, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 3: 1: 1.
Compositions 4: for the compositions of embodiment 4 preparations, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 7: 1.5: 1.5.
Compositions 5: for the compositions of embodiment 5 preparations, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 4: 1: 1.
Compositions 6: for the compositions of embodiment 6 preparations, know that by its proportioning raw materials the ratio of Rhizoma Smilacis Bockii, Rhizoma Corydalis and the Radix Astragali is 7: 1.5: 2.5.
To adding in compositions 1-6 0.5%CMC-Na solution preparation to become the medicinal liquid of desired concn, standby.
Positive control drug: gynecological's FUKE QIANJIN PIAN: Zhuzhou Qianjin Pharmacy Co., Ltd, lot number: 200706044; Rhizoma Smilacis Bockii capsule: Hubei Fu Ren Pharmaceutical Co., Ltd, lot number: 20070804; Penicillin, Huabei Pharmaceutic Co., Ltd produces, lot number: B0508004.
2, laboratory animal
SD rat, male and female half and half, 150-170g.
3, experimental technique
Adopt lumbar injection pentobarbital sodium 40mg.kg-1 anesthetized rat, after first brushing iodine tincture and disinfecting in alcohol again, in rat abdomen, cut the osculum of 1cm left and right, the cotton balls of sterilize (autoclave sterilization again of first weighing) (each heavily about 20mg) is inserted subcutaneous, skin suture immediately, and sterilize with mycillin liquid 0.2ml.In performing the operation, started administration the same day, each group is pressed 15m1.kg-1 gavage, successive administration 7d, 2h after last administration, opens former otch, and cotton balls is taken out in the lump together with connective tissue around, reject fatty tissue, oven dry is weighed, and what the amount of weighing was deducted to former cotton balls measures out granulation weight.
4, experimental result
In Table 1.The compositions that table 1 is got thing, Rhizoma Corydalis extract proportionings different from Radix Astragali extract for Rhizoma Smilacis Bockii on rat granuloma swollen affect result.
5, experiment brief summary
By granulation weight contrast in table 1, known, give rats gavaged compositions 1-6, the swollen model of rat granuloma is all had to obvious antiinflammatory action.
6, experiment conclusion
The compositions of Rhizoma Smilacis Bockii extract, Rhizoma Corydalis extract and Radix Astragali extract has obvious antiinflammatory action, and shows synergism, and three's weight ratio is:
Rhizoma Smilacis Bockii 60～120: the Radix Astragali 10～40: Rhizoma Corydalis 10～40.
Table 1
Note: compare * P < 0.05 with negative control group, * * P < 0.01; With the FUKE QIANJIN PIAN comparison Δ P < of gynecological 0.05, Δ Δ P < 0.01; Compare #P < 0.05, ##P < 0.01, ###P < 0.01 with Rhizoma Smilacis Bockii capsule.
It should be noted that, the present invention not only comprises tablet, granule, pill or capsule, can also be other oral solid formulations and slow control diluent, and these are all protection scope of the present invention.
The technical scheme above embodiment of the present invention being provided is described in detail, applied specific case herein the principle of the embodiment of the present invention and embodiment are set forth, the explanation of above embodiment is only applicable to help to understand the principle of the embodiment of the present invention; , for one of ordinary skill in the art, according to the embodiment of the present invention, in the specific embodiment and range of application, all will change, in sum, this description should not be construed as limitation of the present invention meanwhile.
Claims (6)
1. a medicine for the treatment of chronic pelvic inflammatory disease, is characterized in that, its raw material weight umber ratio is:
16.5～33.0 parts of Rhizoma Smilacis Bockii effective sites, 2.19～8.76 parts of Radix Astragali effective sites, 0.65～2.6 part of Rhizoma Corydalis effective site;
Described Rhizoma Smilacis Bockii effective site is Rhizoma Smilacis Bockii total flavones and total saponins, and described Radix Astragali effective site is astragaloside, and described Rhizoma Corydalis effective site is Rhizoma Corydalis total alkaloids.
2. the medicine for the treatment of chronic pelvic inflammatory disease according to claim 1, is characterized in that, its raw material weight umber ratio is:
24.75 parts of Rhizoma Smilacis Bockii effective sites, 4.38 parts of Radix Astragali effective sites, 1.3 parts of Rhizoma Corydalis effective sites.
3. a preparation method for chronic pelvic inflammatory disease medicine described in claim 1, is characterized in that:
The step of producing and above-mentioned three kinds of effective sites being mixed and made into tablet, granule, pill or capsule that comprises the producing of the producing of Rhizoma Smilacis Bockii effective site, Radix Astragali effective site, Rhizoma Corydalis effective site;
Described Rhizoma Smilacis Bockii effective site, Radix Astragali effective site and Rhizoma Corydalis effective site to produce step as follows:
By Rhizoma Smilacis Bockii, the Radix Astragali or Rhizoma Corydalis after pulverizing, by alcohol-water solution, carry out reflux, extract, and filter, obtain filtrate;
Described filtrate is concentrated and remove alcohol, obtain concentrated solution;
By after the capable polyamide column of described concentrated solution or macroporous resin column or gel chromatographic columns, with alcohol-water solution eluting, collect to obtain eluent;
Described eluent concentrate drying is obtained to its effective site.
4. the preparation method of chronic pelvic inflammatory disease medicine according to claim 3, is characterized in that:
Described Rhizoma Smilacis Bockii effective site produce step, specifically:
Get Rhizoma Smilacis Bockii pulverizing medicinal materials and become coarse powder, adopt 60% alcohol reflux twice, add for the first time 12 times of amount solvent extraction 2h, add for the second time 10 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Rhizoma Smilacis Bockii medical material 5/9 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 in the speed of 2BV/h, the diameter of the post bed of resin be 1: 6 at high proportion, with distilled water, with the flow velocity eluting 6BV of 1.5-2BV/h, to eluent, become colourless, use again the flow velocity drip washing 6BV of 20% ethanol 1.5-2BV/h, discard, flow velocity eluting 5BV with 70% ethanol 1.5-2BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is drying to obtain Rhizoma Smilacis Bockii effective site again, yield must not be less than 1.0%.
5. the preparation method of chronic pelvic inflammatory disease medicine according to claim 3, is characterized in that:
Described Radix Astragali effective site produce step, specifically:
Get Milkvetch Root decoction pieces, adopt water reflux, extract, twice, add for the first time 10 times of amount solvent extraction 2h, add for the second time 8 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the medicinal liquid that concentration is 0.2g/ml;
The heavy dried resin of Milkvetch Root 5/8 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 1BV/h, the diameter of the post bed of resin be 1: 6 at high proportion, wash 3BV, flow velocity 2BV/h, with 70% ethanol elution 3BV, flow velocity 2BV/h, collect 70% alcohol eluen, reclaim ethanol;
At 70 ℃, eluent density is concentrated into 1.00-1.10, into and out of air port temperature, be respectively that under 150-170 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Radix Astragali effective site, yield must not be less than 1.0%.
6. the preparation method of chronic pelvic inflammatory disease medicine according to claim 3, is characterized in that:
Described Rhizoma Corydalis effective site produce step, specifically:
Get corydalis tuber medicinal material and be ground into coarse powder, adopt 60% alcohol reflux twice, add for the first time 6 times of amount solvent extraction 2h, add for the second time 4 times of amount solvent extraction 1.5h, merge extractive liquid,, filters to obtain filtrate;
Described filtrate recycling ethanol to without alcohol taste, and is prepared into the concentrated solution that concentration is 0.4g/ml;
The heavy dried resin of corydalis tuber medicinal material 1/4 and concentrated solution be take to the macroporous adsorptive resins that model is AB-8 on the flow velocity of 2BV/h, the diameter of the post bed of resin be 1: 6 at high proportion, with distilled water eluting 6BV, flow velocity is 2-3BV/h, then discards with 10% ethanol drip washing 2BV, and flow velocity is 2-3BV/h, 70% ethanol elution 5BV, flow velocity is 2-3BV/h, collects 70% alcohol eluen, reclaims ethanol;
At 70 ℃, eluent density is concentrated into 0.90-1.05, into and out of air port temperature, be respectively that under 150-190 ℃ and 80-95 ℃, nebulizer rotating speed 40MHz, peristaltic pump sample introduction speed 10-20Hz, cyclonic separation pressure reduction 0.6MPa condition, spraying is dry again, obtain Rhizoma Corydalis effective site, yield must not be less than 0.03%.
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