CN104043100A - Use of slug protein in preparing medicines for treating asthma - Google Patents
Use of slug protein in preparing medicines for treating asthma Download PDFInfo
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- CN104043100A CN104043100A CN201410330154.7A CN201410330154A CN104043100A CN 104043100 A CN104043100 A CN 104043100A CN 201410330154 A CN201410330154 A CN 201410330154A CN 104043100 A CN104043100 A CN 104043100A
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- treatment asthma
- slug protein
- asthma medicine
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a use of a slug protein in preparing medicines for treating asthma and belongs to the technical field of the medicines. In order to solve the problems of the existing medicines for treating asthma, the slug protein is combined with appropriate medical accessories in use. The invention provides a new use of the slug protein in preparing the medicines for treating the asthma. Designed for special asthma symptoms, the content of the extracted and purified slug protein is 92-99%, and then the slug protein of the purity is prepared into medicines in various forms; the medicines are clear in ingredients, good in consistency, controllable in quality, good in curative effect on asthma symptoms and convenient to use.
Description
Technical field
The invention belongs to medical technical field, relate to a kind of pharmaceutical production technique, the application of especially a kind of slug albumen in preparation treatment asthma medicine.
Background technology
Asthma is current comparatively common one of disease of human survival that affects in the world, is the disease being caused by many reasons.
There is at present multiple medicine, Chinese medicine is because decoction difficulty affects its clinical use, and confirmed that at present slug is effective in cure for asthma, and in current technique, not yet have the technical scheme of employing to make slug medicine, in suitability for industrialized production, be difficult to guarantee concordance and the quality control of product.
Summary of the invention
The problem existing for existing treatment asthma medicine, the present invention is combined with slug protein with suitable pharmaceutical necessities, the new purposes of a kind of slug protein in preparation treatment asthma medicine is provided.
Treatment cervical cancer medicine of the present invention is prepared according to following step:
One, extract slug protein:
(1) the dry slug of 1000g is put into room temperature expanding apparatus, be filled with 1 ~ 10kg nitrogen, 0.1 ~ 0.5kg ammonia, be forced into 0.2 ~ 1.0Mpa, time 10 ~ 30min, gas in abrupt release device then, slug is by expanded pulverizing;
(2) puffing powder broken material joins in expanded solvents system extraction element, is filled with 200 ~ 2000g carbon dioxide, 100 ~ 500g ethanol and 100 ~ 200g dimethyl formamide, is forced into 1 ~ 8Mpa, 10 ~ 25 ℃ of temperature, time 10 ~ 60min, then release pressure, to normal pressure, is collected mixed liquor;
(3) mixed liquor is separated with 1500 ~ 5000r/min with seperator, collects liquid phase;
(4) collect liquid phase and filter with membrane aperture 0.5 ~ 50 μ m Microfilter, obtain filtrate;
(5) filtrate is injected in the solvent resistant column of saphacryls-100HR and filters under the condition of pH=3 ~ 12;
(6) filtrate is dissolved in the dicyandiamide solution being comprised of according to the volume ratio of 4:3:3:2 chloroform-ethanol-methanol-water, pump in ultrasonic standing wave preparative liquid chromatogram, ultrasonic standing wave power 0.2 ~ 5.0KW, frequency 500 ~ 1000kHz, flow velocity 50 ~ 500mL/min, UV-detector wavelength 280nm, separation and purification goes out slug protein;
(7) purification slug protein, with vacuum freeze drier under vacuum 200 ~ 800Kpa, temperature-50 ~ 30 ℃, the condition of time 6 ~ 24h, is dried to the freeze-dried protein finished product of moisture content 5 ~ 8%;
Two, mix: according to proportioning, mix, be processed into the treatment asthma medicine of various dosage forms.
Slug protein is the biological activity protein of extraction separation and purification from slug, molecular structure is clear, medicinal potency ratio is higher, high specificity, toxic reaction relatively a little less than, be difficult in vivo producing accumulating, there is the awareness of molecule with the affinity of body inner recipient, and almost there is no alienation biological metabolism toxicity.
The present invention is directed to asthma characteristic symptom and design, a kind of slug protein content that extracts purification reaches 92～99%, then with the slug protein preparation of this purity, becomes the medicine of various dosage forms, this drug ingredient is clear and definite, high conformity, quality controllable, to cervical cancer disease good effect and easy to use.
The specific embodiment: present embodiment is prepared the medicine for the treatment of cervical cancer by the following method:
The dry slug of 1000g is put into room temperature expanding apparatus, be filled with 1 ~ 10kg nitrogen, 0.1 ~ 0.5kg ammonia, be forced into 0.2 ~ 1.0Mpa, time 10 ~ 30min, gas in abrupt release device then, slug is by expanded pulverizing;
(2) puffing powder broken material joins in expanded solvents system extraction element, is filled with 200 ~ 2000g carbon dioxide, 100 ~ 500g ethanol and 100 ~ 200g dimethyl formamide, is forced into 1 ~ 8Mpa, 10 ~ 25 ℃ of temperature, time 10 ~ 60min, then release pressure, to normal pressure, is collected mixed liquor;
(3) mixed liquor is separated with 1500 ~ 5000r/min with seperator, collects liquid phase;
(4) collect liquid phase and filter with membrane aperture 0.5 ~ 50 μ m Microfilter, obtain filtrate;
(5) filtrate is injected in the solvent resistant column of saphacryls-100HR and filters under the condition of pH=3 ~ 12;
(6) filtrate is dissolved in the dicyandiamide solution being comprised of according to the volume ratio of 4:3:3:2 chloroform-ethanol-methanol-water, pump in ultrasonic standing wave preparative liquid chromatogram, ultrasonic standing wave power 0.2 ~ 5.0KW, frequency 500 ~ 1000kHz, flow velocity 50 ~ 500mL/min, UV-detector wavelength 280nm, separation and purification goes out slug protein;
(7) purification slug protein, with vacuum freeze drier under vacuum 200 ~ 800Kpa, temperature-50 ~ 30 ℃, the condition of time 6 ~ 24h, is dried to the freeze-dried protein finished product of moisture content 5 ~ 8%;
(8) get above-mentioned freeze-dried protein 10 ~ 15g, mix with other preparations, stirring and evenly mixing, inserts in mould, fixing rear molding slug protein pill, the packed products of taking out.
Therapeutic Method: oral, each potion, takes for one day twice.Taking continuously 60 days is a course for the treatment of.
Therapeutic evaluation:
Cure: Stable asthma, cure completely;
Alleviate: asthma attack frequency obviously reduces, and the frequency reduces more than 30%;
Improve: asthma attack frequency reduces, and the frequency reduces lower than 50%;
Invalid: not have any variation.
The Chinese medicine preparation making by the embodiment of the present invention is treated 15 routine cases and is shown: after the course for the treatment of (60 days), symptom (cure+alleviate) 12 people that are clearly better, account for 80%; 3 examples are (improvement) not too obviously, accounts for 20%; Ineffective Cases 0.
Below by concrete case, Chinese medicine composition curative effect provided by the invention is done to further checking.
Case: Hu, man, 38 years old, suffer from out of breath, asthma more than ten years, through being diagnosed as asthma, in many ways seek medical advice, take multiple Chinese and western drugs DeGrain, take a course for the treatment of of Chinese medicine preparation of embodiment, symptom takes an evident turn for the better, and after the course for the treatment of, is almost recovered.
Above embodiment is only for the present invention is further illustrated, and scope of the present invention is not subject to act enforcement side
The limitation of formula.
Claims (4)
1. the application of slug albumen in preparation treatment asthma medicine, is characterized in that described slug protein obtains by the following method:
(1) the dry slug of 1000g is put into room temperature expanding apparatus, be filled with 1 ~ 10kg nitrogen, 0.1 ~ 0.5kg ammonia, be forced into 0.2 ~ 1.0Mpa, time 10 ~ 30min, gas in abrupt release device then, slug is by expanded pulverizing;
(2) puffing powder broken material joins in expanded solvents system extraction element, is filled with 200 ~ 2000g carbon dioxide, 100 ~ 500g ethanol and 100 ~ 200g dimethyl formamide, is forced into 1 ~ 8Mpa, 10 ~ 25 ℃ of temperature, time 10 ~ 60min, then release pressure, to normal pressure, is collected mixed liquor;
(3) mixed liquor is separated with 1500 ~ 5000r/min with seperator, collects liquid phase;
(4) collect liquid phase and filter with membrane aperture 0.5 ~ 50 μ m Microfilter, obtain filtrate;
(5) filtrate is injected in the solvent resistant column of saphacryls-100HR and filters under the condition of pH=3 ~ 12;
(6) filtrate is dissolved in dicyandiamide solution, pump in ultrasonic standing wave preparative liquid chromatogram, ultrasonic standing wave power 0.2 ~ 5.0KW, frequency 500 ~ 1000kHz, flow velocity 50 ~ 500mL/min, UV-detector wavelength 280nm, separation and purification goes out slug protein, and described dicyandiamide solution is comprised of according to the volume ratio of 4:3:3:2 chloroform-ethanol-methanol-water;
(7) purification slug protein, with vacuum freeze drier under vacuum 200 ~ 800Kpa, temperature-50 ~ 30 ℃, the condition of time 6 ~ 24h, is dried to the freeze-dried protein finished product of moisture content 5 ~ 8%.
2. the application in preparation treatment asthma medicine according to a kind of slug albumen described in claim 1, is characterized in that described treatment asthma medicine is by the pill of making containing slug protein 5 ~ 10g.
3. the application in preparation treatment asthma medicine according to a kind of slug albumen described in claim 1, is characterized in that described treatment asthma medicine is by the tablet of making containing slug protein 8 ~ 12g.
4. the application in preparation treatment asthma medicine according to a kind of slug albumen described in claim 1, is characterized in that described treatment asthma medicine is by the capsule of making containing slug protein 10 ~ 15g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410330154.7A CN104043100A (en) | 2014-07-13 | 2014-07-13 | Use of slug protein in preparing medicines for treating asthma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410330154.7A CN104043100A (en) | 2014-07-13 | 2014-07-13 | Use of slug protein in preparing medicines for treating asthma |
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| CN104043100A true CN104043100A (en) | 2014-09-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410330154.7A Pending CN104043100A (en) | 2014-07-13 | 2014-07-13 | Use of slug protein in preparing medicines for treating asthma |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593114A (en) * | 2019-02-18 | 2019-04-09 | 河南科技大学 | A kind of Huang slug antibacterial peptide, extracting method and its application |
| CN110272472A (en) * | 2019-07-18 | 2019-09-24 | 河南科技大学 | A kind of resisting porphyromonas gingivalis and tool nucleic acid bacillus polypeptide and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562087A (en) * | 2004-04-15 | 2005-01-12 | 广州康采恩医药有限公司 | Amphioxus preparation for treating lung cancer, chronic bronchitis and bronchial asthma, and preparing method |
| CN102657849A (en) * | 2012-06-02 | 2012-09-12 | 哈尔滨工业大学 | Application of toad maggot protein in medicine used for treating cervical cancer |
-
2014
- 2014-07-13 CN CN201410330154.7A patent/CN104043100A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1562087A (en) * | 2004-04-15 | 2005-01-12 | 广州康采恩医药有限公司 | Amphioxus preparation for treating lung cancer, chronic bronchitis and bronchial asthma, and preparing method |
| CN102657849A (en) * | 2012-06-02 | 2012-09-12 | 哈尔滨工业大学 | Application of toad maggot protein in medicine used for treating cervical cancer |
Non-Patent Citations (1)
| Title |
|---|
| 陈根林: "蛞蝓的药理研究进展", 《北方药学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593114A (en) * | 2019-02-18 | 2019-04-09 | 河南科技大学 | A kind of Huang slug antibacterial peptide, extracting method and its application |
| CN109593114B (en) * | 2019-02-18 | 2020-11-06 | 河南科技大学 | Limax antibacterial peptide, extraction method and application thereof |
| CN110272472A (en) * | 2019-07-18 | 2019-09-24 | 河南科技大学 | A kind of resisting porphyromonas gingivalis and tool nucleic acid bacillus polypeptide and application |
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Application publication date: 20140917 |