CN1813900B - Kadsura longipedunculata lignin extract and its preparing method and use - Google Patents
Kadsura longipedunculata lignin extract and its preparing method and use Download PDFInfo
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Abstract
The present invention relates to a method for preparing southern schisandra berry lignin extract. Said method includes the following steps: (a), distilling Chinese medicinal material southern schisandra berry by using water for 1-6 hr, (b), filtering to obtain residue; (c), decocting residue for 10-60 min, filtering; (d), using ethyl alcohol aqueous solution to extract residue, every time is 0.5-3 hr, filtering to obtain filtrate; (e), making the obtained filtrate be passed through resin adsorption column to obtain effluent, the described resin is selected from polar macroporous adsorption resin; (f) using ethyl alcohol to make elution to obtain eluent; and (g), combining effluent and eluent to obtain the liquid southern schisandra berry lignin extract.
Description
Technical field
The present invention relates to the extraction process field of effective ingredient in Chinese, relate to the extraction and the Preparation method and use of the lignans extract of Fructus Schisandrae Sphenantherae particularly.The invention still further relates to the compositions and the purposes that contain Fructus Schisandrae Sphenantherae lignanoid extract.
Background technology
Fructus Schisandrae Chinensis is the dry mature fruit of magnoliaceae schisandra (Schisandra chinensis) and schisandra chinensis (Schisandra sphenanthera), the former is recorded according to China's pharmacopeia in 2000 is Fructus Schisandrae Chinensis, be commonly called as Radix Schisandrae Bicoloris, the latter records and is Fructus Schisandrae Sphenantherae.
Fructus Schisandrae Sphenantherae is close with the Radix Schisandrae Bicoloris chemical constituent, all contains two parts of ethereal oil and lignanoid.Yet the composition of lignanoid and content between are different.Lignanoid's composition of Radix Schisandrae Bicoloris is: deoxyschizandrin, schisandrin B, schisandrin C, schisandrin, schisantherin B, schisantherin A, schisantherin B, and the main lignanoid composition of Fructus Schisandrae Sphenantherae is: schisantherin A, schisantherin B, schisantherin C, schisantherin D, schisandrin, anwuweizic acid, dl-anwulignan, d-Biao Jiabaxin, assist (Yang Fang etc. such as five fat elements, the West China pharmaceutical journal, 2003,18 (6): 438-440).
Existing bibliographical information Radix Schisandrae Bicoloris alcohol extract has central inhibitory action, can be used for Cure for insomnia (CN03100533.0).The step that has adopted the supercritical CO 2 extraction to pass through distillation, concentrating under reduced pressure, resin absorption and ethanol elution again in this patent application, technology is complicated and cost is high.Yet, do not see the pertinent literature that effective site is separated and evaluation is successful report for the Cure for insomnia of Fructus Schisandrae Sphenantherae.
Song Xiaomei etc. (modern Chinese medicine, 2003 the 5th phases, 74-75 page or leaf) have described the Study on extraction for the Fructus Schisandrae Sphenantherae effective ingredient, conclude that Fructus Schisandrae Sphenantherae extracts with coarse powder, and are best with 4 times of amount alcohol reflux of 80%.
Other people has studied the main component of schisandrol extract for central inhibitory action, identify that it is a schisandrin, route of administration is injection, effectively dosage is greater than 60mg/kg (Zhang Linkui etc., Chinese Academy of Medical Sciences's journal, the 13rd the 1st phase of volume, in February, 1991), and this schisandrin is not the main component of Fructus Schisandrae Sphenantherae, and its content in Fructus Schisandrae Sphenantherae is much smaller than schisantherin A, and therefore China's pharmacopeia selection in 2000 schisantherin A is the index components of Fructus Schisandrae Sphenantherae.The somebody studies the volatile oil of Fructus Schisandrae Chinensis, find its for the central nervous system have excitation (Qi Zhi etc., journal of Beijing Medical University, 1988, the volume 20, No.6).
Chinese crude drug Fructus Schisandrae Sphenantherae with low cost only is 1/5 of Radix Schisandrae Bicoloris.At present to effective extracting method of the effective site of Chinese crude drug Fructus Schisandrae Sphenantherae, and the application in the treatment disease has demand.
Summary of the invention
An object of the present invention is to provide a kind of method for preparing Fructus Schisandrae Sphenantherae lignanoid extract, wherein, remove volatile oil, filter by water distillation medical material, behind the decocting decoct medicinal herbs slag, alcohol reflux again, adsorption and desorption by resin filters, and obtains effective site.This Fructus Schisandrae Sphenantherae lignanoid extract has sedative-hypnotic effect, effectively Cure for insomnia.
Another object of the present invention provides Fructus Schisandrae Sphenantherae lignanoid extract and contains the compositions and the purposes of Fructus Schisandrae Sphenantherae lignanoid extract.
Therefore, in one aspect of the invention, provide a kind of method for preparing Fructus Schisandrae Sphenantherae lignanoid extract, comprised step:
(a) water with 6-15 times of Fructus Schisandrae Sphenantherae medical material weight distilled medical material 1-6 hour;
(b) filter the residue medicinal residues;
(c) decocted medicinal residues 10-60 minute, filter;
(d) volume is that the above ethanol water of medical material amount 5-15 75% weight doubly extracts medicinal residues, each 0.5-3 hour, filters and obtains filtrate;
(e) gained filtrate is crossed 1-3 times of medical material amount resin absorption post, obtain effluent, described resin is selected from polar macroporous adsorption resin;
(f) be the above ethanol elution of amount of resin 1-5 75% weight doubly with volume, obtain eluent;
(g) merge effluent and eluent, obtain liquid Fructus Schisandrae Sphenantherae lignanoid extract.
In a specific embodiment aspect this, the consumption of the aqueous solvent in the step (a) is preferably 8-12 times of medical material weight, most preferably is 8 times.
In another specific embodiment aspect this, preferred reflux, extract, is 4 hours in the step (a).
In another specific embodiment aspect this, the consumption of the middle ethanol of step (d) is preferably 12 times of medical material amount, and ethanol is preferably the ethanol water of 90% weight, preferably repeats (d) step 1-3 time, most preferably is 2 times.
In another specific embodiment aspect this, the resin in the step (e) is the low pole macroporous adsorbent resin preferably.
In another specific embodiment aspect this, the ethanol volume of step (f) is preferably 2 times of amount of resin, and concentration of alcohol is preferably the ethanol water of 95% weight.
In another specific embodiment aspect this, also comprise step:
(h) decompression or normal pressure reclaim solvent to the greatest extent, then 50-100 ℃ of oven dry, obtain solid-state Fructus Schisandrae Sphenantherae lignanoid extract.
In another aspect of the present invention, a kind of Fructus Schisandrae Sphenantherae lignanoid extract with method for preparing is provided, total lignans concentration is 50%-95% weight in this extract, schisantherin A concentration is 5%-45% weight.
In another aspect of the present invention, a kind of pharmaceutical composition of Cure for insomnia is provided, it contains above-mentioned Fructus Schisandrae Sphenantherae lignanoid extract of 1-99% and the pharmaceutically acceptable carrier of 1-99%.
In another aspect of the present invention, the purposes of above-mentioned Fructus Schisandrae Sphenantherae lignanoid extract is provided, this extract is used to prepare the medicine of Cure for insomnia.
Of the present invention also have one aspect, the purposes of above-mentioned Fructus Schisandrae Sphenantherae lignanoid extract is provided, this extract is used to prepare antidepressant medicine.
The specific embodiment
In this article, term " medical material amount " refers to medical material weight.
The present invention is through discovering, lignanoids material in the Fructus Schisandrae Sphenantherae is the effective site of its main Cure for insomnia, one of main effective ingredient is a schisantherin A, has developed the method for effective extraction Fructus Schisandrae Sphenantherae lignanoid extract on this basis, thereby has finished the present invention.According to our research, one of main effective ingredient in Kadsura longipedunculata lignin is a schisantherin A, rather than suggested schisandrin in the previous research.Its route of administration is oral, and it is 5-30mg/kg that maincenter suppresses effective dosage schisantherin A, and Fructus Schisandrae Sphenantherae lignanoid extract is 10-60mg/kg.
Fructus Schisandrae Sphenantherae is generally got it and is dried fruit medicine, or is ground into the coarse powder use.This medical material can be buied from each Fructus Schisandrae Sphenantherae place of production or Chinese medicine shop, for example Haozhou, Anhui medical material market etc.Lignanoid in the Fructus Schisandrae Sphenantherae of various places and schisantherin A content are different, are good with the Fructus Schisandrae Sphenantherae in Hanzhong, Shanglou and Henan, can reach 1.039% and 0.912% respectively.
After Fructus Schisandrae Sphenantherae extracting method of the present invention adopts steam distillation to remove volatile oil, again with high concentration ethanol as extracting reagent, carrying out adsorption and purification with macroporous resin then, is purpose to extract Cure for insomnia effective site, and lignanoid and schisantherin A are as quality control standard.Different with the alcohol reflux of mentioning in previous supercritical carbon dioxide extraction in this area and the document, this effective site is orientated its content of lignanoid as greater than 50%, effective ingredient is that its content of schisantherin A is greater than 5%, and price of medicinal material is cheap, method is easy, and equipment requirements is low, and extract obtained cost is low, lignanoid's purity height, dosage is little.
Resin absorption post of the present invention uses polar macroporous adsorption resin, preferred low pole macroporous adsorbent resin.Representational resin examples comprises: LSA-7 or 10, AB-8, D101 series.Preferred resin is available from the blue dark A-7 of adsorbing material Ls Cable Ltd. in Xi'an.Can use the high concentration ethanol balance earlier before use.Last sample, washing, last sample and elution speed are unrestricted, but preferred per kilogram resin elution speed most preferably is 40ml/min between 10-80ml/min.
The present invention (d) and (g) in employed defecator can be various conventional filtration devices.Filter paper vacuum suction filter for example, porous resin, ultrafilter membrane, molecular sieve, packed column etc.
The lignans extract content of the Central-South fruit of Chinese magnoliavine effective part of the present composition is (as 50-95% more than 50%, preferably be 60-90%), the effective site yield is (based on the medical material gross weight) more than 2% (be that yield is the percentage ratio that extract and medical material drop into, yield 2% i.e. 1 kilogram of Fructus Schisandrae Sphenantherae fruit obtains 20 gram lignans extracts).Through ultraviolet spectrophotometry, detect lignanoid's content in the 258nm place, also available colorimetry detects lignanoid's content in the 570nm place; Through the HPLC method, detect schisantherin A content in the 258nm place, the content of effective site is more than 50% in the extract, and schisantherin A content is more than 5%.Simultaneously, because operating procedure is simple, low for equipment requirements, can be used for commercial production in enormous quantities.
The effective site that the present invention obtains mainly is lignans extract, has the effect of tranquilizing soporific, can be used for Cure for insomnia.The main position toxicity that the present invention obtains is low, can high dose uses and has no side effect.
Fructus Schisandrae Sphenantherae lignanoid extract of the present invention can directly use, or on materia medica acceptable carrier and additive, make pharmaceutical composition.For example add sweeting agent, flavoring agent, coloring agent and antiseptic, tablet, lozenge, water or oil suspension, dispersible powders or the granule of preparation oral administration, Emulsion, hard or soft capsule or syrup or elixir.Tablet contains and the active component that is applicable to the nontoxic acceptable mixed with excipients of making tablet.These excipient can be inert diluents for example, as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; Granulating agent and disintegrating agent, for example alginic acid; Binding agent is as starch, gelatin or arabic gum; And lubricant, as magnesium stearate, stearic acid or Talcum.Tablet can not have sugar-coat or uses the known technology coating, postpones disintegration and absorption in gastrointestinal tract, thereby continuous action is provided in a long time.For example, can use the time-delay material, as glyceryl monostearate or distearin.The preparation that orally uses also can be made into hard gelatin capsule, wherein active component and inert solid diluent mix as calcium carbonate, calcium phosphate or Kaolin, or make Perle, wherein active component and water or oil medium, for example Oleum Arachidis hypogaeae semen, liquid paraffin or mixed with olive oil.
The content of effective scope is 1-99% in the pharmaceutical composition of the present invention, can determine route of administration and dosage according to clinical needs by experienced doctor.
Pharmaceutical composition of the present invention also can contain the composition of other treatment insomnia, the conventional sleeping pill pentobarbital sodium that for example can get at present etc. or other Chinese medicine preparation.
Pharmaceutical composition of the present invention can be used with various approach, and for example oral, injection, Transdermal absorption are preferably oral.
The invention effect
Use preparation method of the present invention, can obtain containing the Fructus Schisandrae Sphenantherae lignanoid extract of high concentration lignanoid.Fructus Schisandrae Sphenantherae lignanoid extract of the present invention has the effect of significant Cure for insomnia, can play synergism with pentobarbital.
Embodiment
The extraction and the detection of embodiment 1-4 Kadsura longipedunculata lignin
1. material and instrument
Material: Fructus Schisandrae Sphenantherae (, identifying) through professor Wu Qinan of crude drug teaching and research room of Nanjing University of Traditional Chinese Medicine available from medical material market, Bo continent, Anhui; Macroporous resin (changing the adsorbing material company limited) available from the blue deep friendship in Xi'an; Ethanol (available from Suzhou brewery of Wu).
Instrument: multi-function extractor (Wujin Meng He pharmaceutical machine factory); Tianjin, island UV-1240 spectrophotometer; Tianjin, island LC-10ATVP high performance liquid chromatograph.
Embodiment 1
Get 50kg Fructus Schisandrae Sphenantherae dry fruit, pulverize, carried out vapor distillation 3 hours, filter, obtain medicinal residues with the water of 10 times of medical material amounts.Medicinal residues filter with the water boiling and extraction of 6 times of medical material amounts 30 minutes, discard filtrate (repeating 2 times).90% alcohol reflux of 10 times of medical material amounts extracts twice altogether, each 1 hour, merge ethanol extract twice, filter, obtain the 15L medicinal liquid, the gained medicinal liquid is crossed resin absorption post (LSA-10), and resin demand is 1 times of medical material amount, and medicinal liquid is used 95% ethanol elution after having crossed the post bed, collect ethanol liquid (comprise and flow through liquid and eluent), filter, reclaim ethanol, water bath method, 60 ℃ of oven dry get Fructus Schisandrae Sphenantherae lignanoid extract.
Embodiment 2
Get 50kg Fructus Schisandrae Sphenantherae dry fruit, pulverize, carried out vapor distillation 4 hours, filter, obtain medicinal residues with the water of 8 times of medical material amounts.Medicinal residues filter with the water boiling and extraction of 6 times of medical material amounts, discard filtrate (repeating 1 time).95% alcohol reflux of 8 times of medical material amounts extracts twice altogether, each 1.5 hours, merge ethanol extract twice, filter, obtain the 13L medicinal liquid, the gained medicinal liquid is crossed resin absorption post (LSA-7), and resin demand is 1.5 times of medical material amount, and medicinal liquid is used 95% ethanol elution after having crossed the post bed, collect ethanol liquid (comprise and flow through liquid and eluent), filter, reclaim ethanol, water bath method, 70 ℃ of oven dry get Fructus Schisandrae Sphenantherae lignanoid extract.
Embodiment 3
Get 50kg Fructus Schisandrae Sphenantherae dry fruit, pulverize, carried out vapor distillation 5 hours, filter, obtain medicinal residues with the water of 12 times of medical material amounts.Medicinal residues filter with the water boiling and extraction of 10 times of medical material amounts, discard filtrate (repeating 2 times).85% alcohol reflux of 12 times of medical material amounts extracts twice altogether, each 1 hour, merge ethanol extract twice, filter (crossing the neutral alumina adsorption column), obtain the 19L medicinal liquid, the gained medicinal liquid is crossed resin absorption post (LSA-7), and resin demand is 2 times of medical material amount, and medicinal liquid is used 95% ethanol elution after having crossed the post bed, collect ethanol liquid (comprise and flow through liquid and eluent), filter, reclaim ethanol, water bath method, 80 ℃ of oven dry get Fructus Schisandrae Sphenantherae lignanoid extract.
The detection of total lignans and schisantherin A content in embodiment 4 Fructus Schisandrae Sphenantherae lignanoid extracts
1. instrument: ultraviolet-uisible spectrophotometer (UV-1240, day island proper Tianjin Suzhou factory of company) HPLC (LC-10ATVP, day island proper Tianjin Suzhou factory of company)
2. experimental technique:
Total lignans: accurate each 80 microlitre of schisantherin A reference substance solution, need testing solution and methanol of drawing, nor-alcohol volatilizees, accurate respectively again 10% chromotropic acid clear aqueous solution 1ml, sulphuric acid 6ml, the distilled water 3ml of adding, shake up, put boiling water bath heating 30 minutes, cooling is a blank with the methanol pipe rapidly, spectrophotometric 570nm place measures absorbance, calculates lignanoid's content.
Schisantherin A: the HPLC method, mobile phase methanol-water (80: 20) detects wavelength 258nm, flow velocity 1ml/ branch, 20 ℃ of column temperatures.
3. the result is as follows:
Total lignans assay schisantherin A
Embodiment 1 58% 7%
Embodiment 2 65% 9%
Embodiment 3 56% 6%
Three batches 50 kilograms medical material is through extracting, the final extraction ratio of main effective site Kadsura longipedunculata lignin is (total lignans that contains in medical material is 3%) more than 60%, the effective site yield is (relatively medical material input amount) more than 2%, after testing in the extract content of effective site at (total lignans in extract shared percentage by weight) more than 50%.
Embodiment 5 Fructus Schisandrae Sphenantheraes have synergism to the sleep that threshold measures pentobarbital sodium down
Experiment material
1. medicine and reagent
Stable 10mg/kg is mixed with the 0.5mg/ml transfusion
Fructus Schisandrae Sphenantherae decocting liquid 1g crude drug/kg (medical material decocting liquid concentrates)
Kadsura longipedunculata lignin 55mg/kg (lignans extracts of embodiment 2 preparations)
Fructus Schisandrae Sphenantherae polysaccharide 77mg/kg (medical material water decoction-alcohol sedimentation, precipitation is water-soluble go out) except that protein purification
Fructus Schisandrae Sphenantherae volatile oil 70 μ l/kg (steam distillation) Fructus Schisandrae Sphenantherae hydrotrope 92mg/kg (steam distillation remove behind the volatile oil decocting liquid concentrate)
Add two of Tween 80s during dissolving; More than each medicine 1g crude drug/kg, be mixed with the 0.2ml/10g mouse stomach.
2. animal
The ICR mice, male SD rat, all available from China Medicine University's Experimental Animal Center, the quality certification number: SCXK (Soviet Union) 2002-0011 number.
3. experimental technique and result
70 of female mices are divided into 7 groups at random, are respectively blank group, positive drug group, each Fructus Schisandrae Sphenantherae group.Gastric infusion 0.2ml/10g, 40 minutes pneumoretroperitoneum injection pentobarbital sodium 20mg/kg (0.1ml/10g).Observe the number of animals of righting reflex loss in the 1h of injection back, and the record time for falling asleep.
The results are shown in Table 1:
Table 1
| Group | Dosage (mg/kg) | N | Sleeping number |
| Blank | 10 | 0 | |
| Stable | 10 | 10 | 10 |
| Lignanoid | 55 | 8 | 7 |
| Polysaccharide | 77 | 8 | 0 |
| Group | Dosage (mg/kg) | N | Sleeping number |
| Volatile oil | 70 | 8 | 1 |
| The hydrotrope | 92 | 8 | 0 |
| Decocting liquid | 1000 (crude drugs) | 8 | 0 |
Conclusion: Kadsura longipedunculata lignin has significant synergism with stable pentobarbital sodium to sub-threshold dose, and main maincenter inhibition effective site is total lignans in the prompting Fructus Schisandrae Sphenantherae.
The hypnosis model routine dose that pentobarbital sodium caused of sub-threshold dose is 20-30mg/kg, can not fall asleep without the mice of other medicines, uses central depressant just can produce sleep simultaneously.
The influence of 6 pairs of spontaneous activity in mice of embodiment
Experiment material
1. medicine and reagent
Fructus Schisandrae Sphenantherae lignanoid extract 50,100, the 200mg/kg of embodiment 1 preparation are mixed with 2.5,5, the solution of 10mg/ml, add two of Tween 80s during dissolving; More than each medicine 0.2ml/10g irritate stomach.
2. animal
The ICR mice, male SD rat, all available from China Medicine University's Experimental Animal Center, the quality certification number: SCXK (Soviet Union) 2002-0011 number.
3. method: 50 of female mices, random packet is respectively blank group, positive drug group, high, medium and low 3 the administration groups of Fructus Schisandrae Sphenantherae lignanoid extract, and gastric infusion 0.2ml/10g observes the interior event trace of mice 2min, and traces after 1 hour.
The results are shown in Table 2.
Table 2
| Group | N | The spontaneous activity number | |
| Blank | 10 | 101.4±49.6 | |
| Fructus Schisandrae Sphenantherae lignanoid extract | 60mg/kg | 10 | 75.5±34.1 |
| 30mg/kg | 10 | 82.6±55.1 | |
| 10mg/kg | 10 | 93.7±41.9 | |
| Stable | 5mg/kg | 10 | 64.5±23.0 * |
*p＜0.05
Conclusion: Kadsura longipedunculata lignin is respectively organized medicine and under this dosage spontaneous activity in mice is not made significant difference, and points out it that normal physiological activity is not had influence.
Embodiment 7 Fructus Schisandrae Sphenantherae lignanoid extracts are to the sleep synergism of sub-threshold dose pentobarbital sodium
Experiment material
1. medicine and reagent
Stable 10mg/kg;
Fructus Schisandrae Sphenantherae lignanoid extract 50,100, the 200mg/kg of embodiment 1 preparation are mixed with 2.5,5, the solution of 10mg/ml, add two of Tween 80s during dissolving; More than each medicine 0.2ml/10g irritate stomach.
2. animal
The ICR mice, male SD rat, all available from China Medicine University's Experimental Animal Center, the quality certification number: SCXK (Soviet Union) 2002-0011 number.
3. method: 50 of female mices, random packet is respectively blank group, positive drug group, high, medium and low 3 the administration groups of Fructus Schisandrae Sphenantherae lignanoid extract, gastric infusion 0.2ml/10g, pneumoretroperitoneum was injected pentobarbital sodium 20mg/kg (0.1ml/10g) in 40 minutes.Observe the number of animals of righting reflex loss in the 1h of injection back, and the record time for falling asleep.
The results are shown in Table 3.
Table 3
| Group | Dosage (mg/kg) | N | Sleeping number |
| Blank | 10 | 0 | |
| Stable | 10 | 10 | 10 |
| Lignanoid's (height) | 60 | 10 | 6 |
| Lignanoid (in) | 30 | 10 | 3 |
| Lignanoid's (low) | 10 | 10 | 1 |
Conclusion: sample has significant synergism for the pentobarbital sodium of sub-threshold dose, and becomes dose dependent, illustrates that it has sedative-hypnotic effect.
Embodiment 8 prolongs the length of one's sleep of pentobarbital sodium
1. medicine and reagent
Stabilize 10mg/kg, be mixed with the solution of 0.5mg/ml;
Fructus Schisandrae Sphenantherae lignanoid extract 50,100, the 200mg/kg of embodiment 1 preparation are mixed with 2.5,5, the solution of 10mg/ml, add three of Tween 80s during dissolving; More than each medicine 0.2ml/10g irritate stomach.
2. animal
The ICR mice, male SD rat, all available from China Medicine University's Experimental Animal Center, the quality certification number: SCXK (Soviet Union) 2002-0011 number.
3. method: 50 of male mices, the random packet of weighing, be respectively blank group, positive drug group, high, medium and low 3 the administration groups of Fructus Schisandrae Sphenantherae lignanoid extract, gastric infusion 0.2ml/10g, 40 minutes pneumoretroperitoneum injection pentobarbital sodium 35mg/kg (0.1ml/10g).Observe the number of animals of righting reflex loss in the 2h of injection back, and record time for falling asleep and recovery time, the dropping asleep latency and the length of one's sleep calculated.
The results are shown in Table 4.
Table 4
| Group | Dosage mg/kg | Number of animals | Dropping asleep latency (min) | The length of one's sleep (min) |
| Blank group | 10 | 4.10±0.62 | 24.67±14.17 | |
| The positive drug group | 10 | 10 | 3.55±0.24 * | 104.4±13.01 ** |
| Lignanoid's (low) | 10 | 10 | 4.43±1.07 | 45.77±10.40 * |
| Lignanoid (in) | 30 | 10 | 3.86±0.30 | 55.72±28.87 ** |
| Lignanoid's (height) | 60 | 10 | 3.61±0.20 * | 74.60±24.62 ** |
*p＜0.05，
**p＜0.01
Conclusion: with the blank group relatively, Fructus Schisandrae Sphenantherae lignanoid extract has the pentobarbital sodium of prolongation length of one's sleep, points out it to have function for assisting sleep preferably.
As mentioned above, method of the present invention can be extracted Fructus Schisandrae Sphenantherae lignanoid extract efficiently, and technology is simple, the active constituent content height.In addition, resulting product has remarkable effect in the treatment for insomnia.
Product of as above having described Fructus Schisandrae Sphenantherae lignanoid extract preparation method disclosed in this invention and having made and uses thereof.But need to understand and wherein comprised variation understood by one of ordinary skill in the art and change.These variations and change are also contained in the scope of the present invention that claim limits.
Claims (10)
1. method for preparing Fructus Schisandrae Sphenantherae lignanoid extract is characterized in that the method comprising the steps of:
(a) water with 6-15 times of Fructus Schisandrae Sphenantherae medical material weight distilled medical material 1-6 hour;
(b) filter the residue medicinal residues;
(c) decocted medicinal residues 10-60 minute, filter;
(d) volume is that the above ethanol water of medical material amount 5-15 75% weight doubly extracts medicinal residues, each 0.5-3 hour, filters and obtains filtrate;
(e) gained filtrate is crossed 1-3 times of medical material amount resin absorption post, obtain effluent, described resin is selected from polar macroporous adsorption resin;
(f) be the above ethanol elution of amount of resin 1-5 75% weight doubly with volume, obtain eluent;
(g) merge effluent and eluent, obtain liquid Fructus Schisandrae Sphenantherae lignanoid extract.
2. the method for claim 1 is characterized in that, the consumption of the aqueous solvent in the described step (a) is 8-12 a times of medical material weight.
3. the method for claim 1 is characterized in that, in the described step (a), and reflux, extract, 3 hours.
4. the method for claim 1 is characterized in that, described method also comprises step:
(h) decompression or normal pressure reclaim solvent to the greatest extent, then 50-100 ℃ of oven dry, obtain solid-state Fructus Schisandrae Sphenantherae lignanoid extract.
5. the method for claim 1 is characterized in that, repeating step (d) 1-3 time.
6. the method for claim 1 is characterized in that, the resin in the described step (e) is selected from the low pole macroporous adsorbent resin.
7. a Fructus Schisandrae Sphenantherae lignanoid extract is characterized in that, total lignans concentration is 50%-95% weight in this extract, and schisantherin A concentration is 5%-45% weight.
8. Fructus Schisandrae Sphenantherae lignanoid extract as claimed in claim 7 is characterized in that, this extract prepares with the described method of claim 1.
9. the pharmaceutical composition of a Cure for insomnia is characterized in that, it contains described Fructus Schisandrae Sphenantherae lignanoid extract of 1-99% claim 7 and the pharmaceutically acceptable carrier of 1-99%.
10. the purposes of the described Fructus Schisandrae Sphenantherae lignanoid extract of claim 7 is characterized in that, this extract is used to prepare the medicine of Cure for insomnia.
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| CN103340407B (en) * | 2013-07-23 | 2015-07-22 | 四川省中医药科学院 | Composition with sleep improving effect as well as preparation method and use of composition |
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