CN102225095A - Effective fraction of plantain seeds as well as preparation method and application thereof - Google Patents
Effective fraction of plantain seeds as well as preparation method and application thereof Download PDFInfo
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- CN102225095A CN102225095A CN2011101725156A CN201110172515A CN102225095A CN 102225095 A CN102225095 A CN 102225095A CN 2011101725156 A CN2011101725156 A CN 2011101725156A CN 201110172515 A CN201110172515 A CN 201110172515A CN 102225095 A CN102225095 A CN 102225095A
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Abstract
The invention discloses an effective fraction of plantain seeds, as well as a preparation method and an application thereof. The effective fraction of plantain seeds is obtained by separating and purifying a plantain seed ethanol extract with a macroporous resin and contains hispidulin A and acteoside; and the weight ratio of hispidulin A to acteoside ranges from 1:1 to 1:5 and the sum weight of hispidulin A and acteoside accounts for 40% to 80% of the total weight of the effective fraction. The effective fraction of plantain seeds, disclosed by the invention, can promote the secretion of insulin and reduce fasting blood-glucose levels and can also effectively reduce blood pressure in animals and reduce blood plasma angiotensin II level, shows good medicinal values in treatment of diabetes and hypertension, and can be used alone or in combination with pharmaceutically-acceptable carriers to prepare pharmaceutical formulations for treating diabetes and/or hypertension.
Description
Technical field
The present invention relates to a kind of Semen Plantaginis effective site and its production and application, specifically, relate to a kind of be used for the treatment of diabetes and hypertensive Semen Plantaginis effective site and preparation method thereof and the application in preparation diabetes and hypertension drug, belong to technical field of Chinese medicines.
Background technology
Current, diabetes and hypertension have become the chronic disease of serious harm modern humans health, its pathogenic factor and mechanism are not fully aware of at present, but any is arranged is relatively to generally acknowledge, being that hypertension and diabetes all are chronic lifelong participation diseases of many reasons, is the result that the metabolism of several systems gets muddled in the body.If be used for the treatment of diabetes and hypertensive drug main chemical synthetic drug at present, these medicines have certain therapeutical effect to diabetes and hypertension, but also be accompanied by simultaneously many side effect, as unusual, the bradycardia of gastral untoward reaction, blood fat and blood sugar level and cough, asthma, certain organ toxicity etc.Simultaneously,, need the long-term follow treatment, so just give government and patient with huge spirit and material pressure because diabetes are relevant often with hypertensive generation.Therefore, from Chinese medicine, seek treatment diabetes and hypertensive new method, will produce good social benefit and economic benefit.
Semen Plantaginis is a conventional Chinese medicine, " Chinese pharmacopoeia (2010 editions) is with the dry mature seed of Plantaginaceae Plantago plant Herba Plantaginis Plantago asiatica L. or the Plantago depressa Willd Plantago depressa Willd. Ji Yuan as Semen Plantaginis, has clearing away heat and promoting diuresis, eliminating dampness by diuresis is treating stranguria, the effect such as eliminate the phlegm that makes eye bright is used for the treatment of acute glomerulonephritis, cough with asthma and icterohepatitis etc. traditionally.But Semen Plantaginis also other Chinese medicines of compatibility is used, and for example the compatibility Semen Plantaginis plays the effect of clearing away heat-damp and promoting diuresis in the compound recipe Decoction of Gentiana for Purging the Liver-fire.But the relevant report that also is not used for the treatment of diabetes and hypertensive effective site and preparation method thereof at present about Semen Plantaginis extract.
Summary of the invention
The purpose of this invention is to provide a kind of be used for the treatment of diabetes and hypertensive Semen Plantaginis effective site and preparation method thereof and its application in preparation diabetes and hypertension drug.
Semen Plantaginis effective site of the present invention, be that separation and purification obtains the Semen Plantaginis ethanol extraction through macroporous resin, contain plain A of Herba Plantaginis and acteoside, and the ratio of the plain A of Herba Plantaginis and the weight of acteoside is 1: 1～1: 5, both weight sums are occupied and are imitated 40～80% of position gross weight.
Further, the plain A of described Herba Plantaginis is 1: 2～1: 4 with the ratio of the weight of acteoside, and both weight sums are occupied 50～70% of effect position gross weight.
It is the extract that 60～90% ethanol water reflux, extract, obtains for 2～6 times through the volume fraction of 2～6 times of volumes that described Semen Plantaginis ethanol extraction is recommended as Semen Plantaginis; Being preferably Semen Plantaginis is the extract that 70～80% ethanol water reflux, extract, obtains for 3～5 times through the volume fraction of 3～5 times of volumes.
The preparation method of described Semen Plantaginis effective site comprises following concrete steps:
A) be the ethanol water reflux, extract, 2～6 times (preferred 3～5 times) of 60～90% (preferred 70～80%) with Semen Plantaginis with the volume fraction of 2～6 times of (preferred 3～5 times) volumes, collect extracting solution, filter, concentrate dried solvent;
B) concentrated extracting solution that step a) is prepared dilutes the suspendible aqueous solution adding HPD-100 macroporous resin column that obtains with the water of the by weight/volume of 5: 1～1: 5 (being preferably 3: 1～1: 3), the reuse volume fraction is that the ethanol water of 20～60% (preferred 30～50%) carries out eluting, collect eluent, concentrate dried solvent.
Described Semen Plantaginis is recommended as the dry mature seed of Plantaginaceae plant Herba Plantaginis (Plantago asiatica L.) and/or Plantago depressa Willd (Plantago depressa Willd.).
Described Semen Plantaginis recommends to be ground into the granule that diameter is less than or equal to 6mm.
The application of described Semen Plantaginis effective site in preparation treatment diabetes and/or hypertension drug preparation.
The application of the compositions that described Semen Plantaginis effective site and pharmaceutically acceptable pharmaceutical carrier are formed in preparation treatment diabetes and/or hypertension drug preparation.
Described pharmaceutical dosage forms can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch; The preferred oral dosage form, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc.Described peroral dosage form can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.Suitable filler comprises cellulose, mannitol, lactose and other similar filler; Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate; Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
The Semen Plantaginis effective site that the present invention obtains can promote insulin secretion, reduces fasting glucose, can effectively reduce the animal blood pressure value simultaneously, reduces the content of the nervous plain II of serum medium vessels, demonstrates good curing diabetes and hypertensive medical value.
Description of drawings
The finger printing that the Semen Plantaginis effective site that Fig. 1 obtains for the present invention obtains under positive ion mode.
The mass spectrum that the Semen Plantaginis effective site that Fig. 2 obtains for the present invention obtains under positive ion mode.
Fig. 3 is the chemical structural formula figure of the plain A of Herba Plantaginis.
The finger printing that the Semen Plantaginis effective site that Fig. 4 obtains for the present invention obtains under negative ion mode.
The mass spectrum that the Semen Plantaginis effective site that Fig. 5 obtains for the present invention obtains under negative ion mode.
Fig. 6 is the chemical structural formula figure of acteoside.
The specific embodiment
Below in conjunction with embodiment the present invention is done further detailed, complete explanation.
Embodiment 1
Semen Plantaginis powder being broken into the granule that average diameter is 4mm, sieving, is 75% ethanol water reflux, extract, 4 times with the volume fraction of 4 times of volumes, collects extracting solution, filters, concentrates dried solvent;
Water with 1: 1 by weight/volume dilutes above-mentioned concentrated extracting solution, obtains the suspendible aqueous solution;
The suspendible aqueous solution that obtains is added the HPD-100 macroporous resin column, is that 40% ethanol water carries out eluting with volume fraction, collects eluent, and concentrated dried solvent promptly gets Semen Plantaginis effective site.
It is an amount of to take by weighing the Semen Plantaginis effective site sample that makes, (V: V) aqueous solution of formic acid and methanol are that 90: 10 blended solution is dissolved in the volumetric flask by volume with 0.1%, be diluted to scale, shake up, make every 1mL solution and contain the 1g sample, and utilize Ultra Performance Liquid Chromatography-GC-MS (UPLC-MS) to carry out fingerprint map analyzing, analysis condition is as follows:
Chromatographic condition: Waters, Acquity UPLC BEH C
18(50 * 2.1mm) chromatographic columns; Mobile phase A is mutually for containing 0.1% (V: the V) aqueous solution of formic acid; Mobile phase B is methanol mutually; Flow velocity is 0.3min; Column temperature is 40 ℃; Sample size is 2 μ L; Detecting pattern is the monitoring of negative ions full scan.
The mass spectrum condition: adopt electric spray ion source, when cation full scan detecting pattern carried out data acquisition, ion source temperature was 120 ℃; The temperature of desolvating is 300 ℃; Nitrogen is for desolvating and taper hole gas, and flow velocity is respectively 500L/h and 50L/h; Capillary voltage is 2.2 kilovolts; Taper hole voltage is 23 volts.When anion full scan detecting pattern carried out data acquisition, ion source temperature was 120 ℃; The temperature of desolvating is 300 ℃; Nitrogen is for desolvating and taper hole gas, and flow velocity is respectively 500L/h and 50L/h; Capillary voltage is 2.5 kilovolts; Taper hole voltage is 32 volts.
Fig. 1 is the finger printing that the Semen Plantaginis effective site that obtains obtains under positive ion mode, and Fig. 2 is the mass spectrum that the Semen Plantaginis effective site that obtains obtains under positive ion mode; In conjunction with Fig. 1 and Fig. 2 as seen: contain the plain A (its chemical structural formula figure sees shown in Figure 3) of Herba Plantaginis in the Semen Plantaginis effective site that obtains.
Fig. 4 is the finger printing that the Semen Plantaginis effective site that obtains obtains under negative ion mode, and Fig. 5 is the mass spectrum that the Semen Plantaginis effective site that obtains obtains under negative ion mode; In conjunction with Fig. 4 and Fig. 5 as seen: contain acteoside (its chemical structural formula figure sees shown in Figure 6) in the Semen Plantaginis effective site that obtains.
In addition, the ratio of analyzing the plain A of the Herba Plantaginis that contains in the Semen Plantaginis effective site record the present embodiment preparation and the weight of acteoside is 1: 3, and both weight sums are occupied and imitated 60% of position gross weight.
Embodiment 2
Semen Plantaginis powder being broken into the granule that average diameter is 1mm, sieving, is 60% ethanol water reflux, extract, 5 times with the volume fraction of 3 times of volumes, collects extracting solution, filters, concentrates dried solvent;
Water with 1: 3 by weight/volume dilutes above-mentioned concentrated extracting solution, obtains the suspendible aqueous solution;
The suspendible aqueous solution that obtains is added the HPD-100 macroporous resin column, is that 50% ethanol water carries out eluting with volume fraction, collects eluent, and concentrated dried solvent promptly gets Semen Plantaginis effective site.
The analysis showed that: the plain A of the Herba Plantaginis that contains in the prepared Semen Plantaginis effective site of present embodiment is 1: 2 with the ratio of the weight of acteoside, and both weight sums are occupied and imitated 50% of position gross weight.
Embodiment 3
Semen Plantaginis powder being broken into the granule that average diameter is 6mm, sieving, is 80% ethanol water reflux, extract, 3 times with the volume fraction of 5 times of volumes, collects extracting solution, filters, concentrates dried solvent;
Water with 1: 2 by weight/volume dilutes above-mentioned concentrated extracting solution, obtains the suspendible aqueous solution;
The suspendible aqueous solution that obtains is added the HPD-100 macroporous resin column, is that 30% ethanol water carries out eluting with volume fraction, collects eluent, and concentrated dried solvent promptly gets Semen Plantaginis effective site.
The analysis showed that: the plain A of the Herba Plantaginis that contains in the prepared Semen Plantaginis effective site of present embodiment is 1: 4 with the ratio of the weight of acteoside, and both weight sums are occupied and imitated 70% of position gross weight.
Embodiment 4
Select healthy male mice for use, (22 ± 2) g is divided into normal group and modeling group at random, modeling group lumbar injection streptozotocin 150mg/kg, cut tail behind the 48h and survey random blood sugar, the mice that the screening blood glucose value surpasses 10.1mmol/l enters experiment, and is divided into model group and administration group.Administration group administration every day Semen Plantaginis effective site 90mg/kg.In 4 weeks of continuous oral gastric infusion, after administration finishes, measure mice serum insulin and fasting blood sugar.Experimental data is carried out variance analysis, and the result represents with x ± s.
Table 1 Semen Plantaginis effective site is to the blood sugar reducing function of type i diabetes mice
| Dosage (mg/kg) | Serum insulin content (IU/mL) | Fasting blood sugar (mmol/L) | |
| Normal group | 24.56±4.21 | 5.31±0.27 | |
| Model group | 13.50±2.22 ### | 29.94±5.18 ### | |
| The administration group | 90，po | 17.38±2.68 ** | 22.02±8.51 * |
Annotate:
###P＜0.001 is compared with normal group;
*P＜0.05,
*P＜0.01 is compared with model group.
By table 1 as seen: compare with normal group, model group mice fasting blood sugar significantly raises, and type i diabetes model modeling success is described.Compare with model group, Semen Plantaginis effective site has significant rising serum insulin content and reduces the effect of fasting glucose, has demonstrated the effect of good curing diabetes.
Embodiment 5
Select original hypertensive rat (SHR) for use, (200 ± 20) g is divided into model group and administration group at random.Administration group administration every day Semen Plantaginis effective site 45mg/kg.In 2 weeks of continuous oral gastric infusion, after administration finishes, measure SHR rat blood pressure value and Angiotensin II level.Experimental data is carried out variance analysis, and the result represents with x ± s.
Table 2 Semen Plantaginis effective site is to the hypotensive effect of original hypertensive rat
| Dosage (mg/kg) | Pressure value (mmHg) | Angiotensin II content (pg/mL) | |
| Model group | 231.13±4.89 | 2811.43±3309.49 | |
| The administration group | 45，po | 213.68±5.19 *** | 1041.95±227.36 * |
Annotate:
*P＜0.05,
* *P＜0.001 is compared with model group.
By table 2 as seen: compare with model group, Semen Plantaginis effective site has the effect of the nervous plain II content of the value of bringing high blood pressure down and serum medium vessels significantly, has demonstrated the hypertensive effect of good curing.
Embodiment 6
Select healthy male wistar rat for use, (200 ± 20) g, be divided into normal group and high fat group (88% normal diet+10% Adeps Sus domestica+2% cholesterol) at random, high fat group lumbar injection streptozotocin 35mg/kg, cut tail after five days and survey random blood sugar, the rat that the screening blood glucose value surpasses 16.7mmol/l enters experiment, and is divided into model group, administration group 1 (Semen Plantaginis crude product group) and administration group 2 (Semen Plantaginis effective site group).Administration group administration 1 every day Semen Plantaginis crude product 150mg/kg, administration group administration 2 every day Semen Plantaginis effective site 45mg/kg.In 4 weeks of continuous oral gastric infusion, after administration finishes, measure rat blood sugar value, serum insulin levels, pressure value and Angiotensin II level.Experimental data is carried out variance analysis, and the result represents with x ± s.
Table 3 Semen Plantaginis effective site is to blood sugar lowering and the hypotensive effect of type ii diabetes in conjunction with Hypertensive Rats
Annotate:
##P＜0.01,
###P＜0.001 is compared with normal group;
*P＜0.05,
*P＜0.01 is compared with model group.Wherein administration group 1 is a Semen Plantaginis crude product group, and administration group 2 is a Semen Plantaginis effective site group.
By table 3 as seen: compare with normal group, model group rat fasting blood-glucose value and pressure value all significantly raise, and illustrate that type ii diabetes is in conjunction with hypertension model modeling success.Simultaneously, compare with model group, Semen Plantaginis effective site has significant rising serum insulin content, reduces the rat blood sugar value, and the effect of the value of bringing high blood pressure down and the nervous plain II content of serum medium vessels, demonstrated good curing diabetes and hypertensive effect, and drug effect is better than Semen Plantaginis crude product.
Claims (10)
1. Semen Plantaginis effective site, it is characterized in that: separation and purification obtains through macroporous resin by the Semen Plantaginis ethanol extraction, contain plain A of Herba Plantaginis and acteoside, and the plain A of Herba Plantaginis is 1: 1～1: 5 with the ratio of the weight of acteoside, and both weight sums are occupied 40～80% of effect position gross weight.
2. Semen Plantaginis effective site according to claim 1 is characterized in that: the plain A of described Herba Plantaginis is 1: 2～1: 4 with the ratio of the weight of acteoside, and both weight sums are occupied 50～70% of effect position gross weight.
3. Semen Plantaginis effective site according to claim 1 is characterized in that: described Semen Plantaginis ethanol extraction is a Semen Plantaginis through the volume fraction of 2～6 times of volumes is the extract that 60～90% ethanol water reflux, extract, obtains for 2～6 times.
4. Semen Plantaginis effective site according to claim 3 is characterized in that: described Semen Plantaginis ethanol extraction is a Semen Plantaginis through the volume fraction of 3～5 times of volumes is the extract that 70～80% ethanol water reflux, extract, obtains for 3～5 times.
5. the preparation method of the described Semen Plantaginis effective site of claim 1 is characterized in that, comprises following concrete steps:
A) be 60～90% ethanol water reflux, extract, 2～6 times with Semen Plantaginis with the volume fraction of 2～6 times of volumes, collect extracting solution, filter, concentrate dried solvent;
B) concentrated extracting solution that step a) is prepared dilutes the suspendible aqueous solution adding HPD-100 macroporous resin column that obtains with the water of 5: 1～1: 5 by weight/volume, the reuse volume fraction is that 20～60% ethanol water carries out eluting, collect eluent, concentrate dried solvent.
6. the preparation method of Semen Plantaginis effective site according to claim 5 is characterized in that, comprises following concrete steps:
A) be 70～80% ethanol water reflux, extract, 3～5 times with Semen Plantaginis with the volume fraction of 3～5 times of volumes, collect extracting solution, filter, concentrate dried solvent;
B) concentrated extracting solution that step a) is prepared dilutes the suspendible aqueous solution adding HPD-100 macroporous resin column that obtains with the water of 3: 1～1: 3 by weight/volume, the reuse volume fraction is that 30～50% ethanol water carries out eluting, collect eluent, concentrate dried solvent.
7. the preparation method of Semen Plantaginis effective site according to claim 5 is characterized in that: described Semen Plantaginis is the dry mature seed of Plantaginaceae plant Herba Plantaginis and/or Plantago depressa Willd.
8. the preparation method of Semen Plantaginis effective site according to claim 5 is characterized in that: described Semen Plantaginis powder is broken into the granule that diameter is less than or equal to 6mm.
9. the application of each described Semen Plantaginis effective site in preparation treatment diabetes and/or hypertension drug preparation in the claim 1 to 8.
10. the application of the compositions that each described Semen Plantaginis effective site and pharmaceutically acceptable pharmaceutical carrier are formed in the claim 1 to 8 in preparation treatment diabetes and/or hypertension drug preparation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105796674A (en) * | 2014-12-31 | 2016-07-27 | 上海中医药大学 | Traditional Chinese medicine composition for preventing and treating metabolic syndrome |
| CN111450148A (en) * | 2020-06-02 | 2020-07-28 | 中国科学院长春应用化学研究所 | Active part of semen plantaginis and preparation method thereof |
| CN111773352A (en) * | 2019-09-20 | 2020-10-16 | 厦门医学院 | Application of turmeric and plantain seed composition in preparation of pharmaceutical composition for reducing blood sugar and blood pressure |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513862A (en) * | 2002-07-19 | 2004-07-21 | 郭绪林 | Preparation process of plantago fotal glycocide and its application in medicine |
-
2011
- 2011-06-23 CN CN 201110172515 patent/CN102225095B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1513862A (en) * | 2002-07-19 | 2004-07-21 | 郭绪林 | Preparation process of plantago fotal glycocide and its application in medicine |
Non-Patent Citations (2)
| Title |
|---|
| 万茵等: "车前子总黄酮的纯化及其成分的液相质谱联用分析", 《食品科学》, vol. 29, no. 2, 28 February 2008 (2008-02-28), pages 328 - 332 * |
| 张杰等: "车前子对高脂血症大鼠血脂水平及抗氧化作用的影响", 《中国新药杂志》, no. 03, 30 March 2005 (2005-03-30) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105796674A (en) * | 2014-12-31 | 2016-07-27 | 上海中医药大学 | Traditional Chinese medicine composition for preventing and treating metabolic syndrome |
| CN105796674B (en) * | 2014-12-31 | 2020-08-07 | 上海中医药大学 | Traditional Chinese medicine composition for preventing and treating metabolic syndrome |
| CN111773352A (en) * | 2019-09-20 | 2020-10-16 | 厦门医学院 | Application of turmeric and plantain seed composition in preparation of pharmaceutical composition for reducing blood sugar and blood pressure |
| CN111450148A (en) * | 2020-06-02 | 2020-07-28 | 中国科学院长春应用化学研究所 | Active part of semen plantaginis and preparation method thereof |
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