CN108904675A - A method of quickly preparing Rhizoma Paridis extract - Google Patents
A method of quickly preparing Rhizoma Paridis extract Download PDFInfo
- Publication number
- CN108904675A CN108904675A CN201810836558.1A CN201810836558A CN108904675A CN 108904675 A CN108904675 A CN 108904675A CN 201810836558 A CN201810836558 A CN 201810836558A CN 108904675 A CN108904675 A CN 108904675A
- Authority
- CN
- China
- Prior art keywords
- extract
- elution
- microwave
- method described
- paris polyphylla
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Diabetes (AREA)
- Alternative & Traditional Medicine (AREA)
- Hematology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of methods for quickly preparing Rhizoma Paridis extract, and steps are as follows：Paris polyphylla pulverizing medicinal materials ethyl alcohol is extracted 3-5 times in Microwave Extraction Apparatus, 30-40min is extracted every time, is filtered after extraction, extracting solution is concentrated under reduced pressure to give medicinal extract；Large pore resin absorption column chromatographic isolation is used after medicinal extract is dissolved with suitable quantity of water, successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 60%, 70%, 80%, 95%, 70% alcohol elution is collected, then is separated through silica gel column chromatography, volume ratio 85 is successively used:1,65:1,45:1,25:1,16:1,4:1 chloroform-methanol elution, collects 16:1 elution fraction, is concentrated to obtain the final product.Microwave extract method provided by the invention is simple to operation, substantially reduces extraction time, simplifies extraction process, and the Paris polyphylla effective part extract of preparation has the function of hemostasis, anti-inflammatory, can be prepared on a large scale.
Description
Technical field
The present invention relates to a kind of methods for quickly preparing Rhizoma Paridis extract.
Background technique
Paris plant is the plant of a kind of great medical value, and the whole world shares 24 kinds, and China possesses 19 kinds, Chinese and Western
Each provinces and regions type in south and resource are extremely abundant.Domestic and foreign scholars take much count of the research to Paris polyphylla, with stronger physiological activity
And unique medical value.Paris polyphylla is written in Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》In recorded with the name of paris polyphylla, be low-grades；And with weight platform
The different names such as root are recorded by history tree ancient books.《Pharmacopoeia of People's Republic of China》Recording the medicine is Yunnan Rhizoma ParidisParis polyphyllaSmith var. yunnanensis (Franch.) Hand.-Mazz. or paris polyphyllaP. polyphyllaThe dry rhizome of Simth var. chinensis (F.) Hara.Also known as paris polyphylla, paris polyphylla, Cao He
Vehicle.Main product is in the Yangtze river basin and southern each province.Autumn excavation, removes fibrous root, cleans, dries.Slice is raw to be used.
Paris polyphylla have clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion the effect of, for too fat to move, abscess of throat, venomous snake bite,
The diseases such as injuries from falls as well, cool breeze twitch.Separation identifies more than 50 compounds from the platymiscium, mainly there is C27 steroidal soap
Glycosides, C21 pregnane glycosides, aliphatic ester, sterol and its glycosides, flavonoid glycoside, 2 moulting hormone of Β and polysaccharide.Wherein 44 kinds of steroid saponin,
80% or more of total compound is accounted for, steroid sapogenin is spirostan alcohols, different spirostan alcohols, taste gonane alcohols and deformation spiral shell
Steroid has very strong physiology and pharmacological activity.Modern pharmacological research Paris polyphylla has hemostasis, eliminating the phlegm and antibacterial, sedation and analgesia, anti-morning
Pregnant killing sperm, the effects of anti-cell is malicious, be clinically used for treatment functional uterine bleeding, neurodermatitis, surgery inflammation and
Tumour etc. has significant curative effect.In China, Paris polyphylla is famous Chinese patent drug Yunnan Baiyao, jidesheng sheyao tablets, Gong Xue Ning glue
The main composition drug of capsule etc..
Summary of the invention
The purpose of the present invention is to provide a kind of methods for quickly preparing Rhizoma Paridis extract.
The present invention is realized by following technical solution：
A method of Rhizoma Paridis extract quickly being prepared, is included the following steps：It with volume fraction is 60- by Paris polyphylla pulverizing medicinal materials
70% ethyl alcohol extracts 3-5 times in Microwave Extraction Apparatus, extracts 30-40min every time, and microwave temperature is 45-55 DEG C, microwave power
For 550-650W, filtered after extraction, extracting solution is concentrated under reduced pressure to give medicinal extract；It is inhaled after medicinal extract is dissolved with suitable quantity of water with macropore
Attached resin column chromatography separation is received successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 60%, 70%, 80%, 95%
Collect 70% alcohol elution, then separated through silica gel column chromatography, successively uses volume ratio 85:1,65:1,45:1,25:1,16:1,4:1
Chloroform-methanol elution, collect 16:1 elution fraction, is concentrated to obtain the final product.
Preferably, in the method, by Paris polyphylla pulverizing medicinal materials volume fraction be 65% ethyl alcohol in Microwave Extraction Apparatus
It extracts 4 times.
Preferably, in the method, 35min will be extracted every time.
It preferably, is 50 DEG C by microwave temperature in the method.
It preferably, is 600W by microwave power in the method.
Preferably, in the method, 1kg Paris polyphylla is extracted using 8-10L solvent.
Preferably, in the method, 1g medicinal extract 7-9mL water is dissolved.
It preferably, is AB-8 type by the large pore resin absorption column in the method.
The technology of the present invention effect：
Microwave extract method provided by the invention is simple to operation, substantially reduces extraction time, simplifies extraction process, preparation
Paris polyphylla effective part extract have the function of hemostasis, it is anti-inflammatory, can be prepared on a large scale.
Specific embodiment
Essentiality content of the invention is specifically introduced below with reference to embodiment.
Embodiment 1
A method of Rhizoma Paridis extract quickly being prepared, is included the following steps：It is 65% by Paris polyphylla pulverizing medicinal materials volume fraction
Ethyl alcohol extracts 4 times in Microwave Extraction Apparatus, extracts 35min every time, and microwave temperature is 50 DEG C, microwave power 600W, extracts knot
It is filtered after beam, extracting solution is concentrated under reduced pressure to give medicinal extract；AB-8 type large pore resin absorption column color is used after medicinal extract is dissolved with suitable quantity of water
Spectrum separation is collected 70% ethyl alcohol and is washed successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 60%, 70%, 80%, 95%
De- position, then separated through silica gel column chromatography, successively use volume ratio 85:1,65:1,45:1,25:1,16:1,4:1 chloroform-methanol
Elution collects 16:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg Paris polyphylla is extracted using 9L solvent, 1g medicinal extract 8mL water is dissolved.
Embodiment 2
A method of Rhizoma Paridis extract quickly being prepared, is included the following steps：It is 60% by Paris polyphylla pulverizing medicinal materials volume fraction
Ethyl alcohol extracts 3 times in Microwave Extraction Apparatus, extracts 30min every time, and microwave temperature is 45 DEG C, microwave power 550W, extracts knot
It is filtered after beam, extracting solution is concentrated under reduced pressure to give medicinal extract；AB-8 type large pore resin absorption column color is used after medicinal extract is dissolved with suitable quantity of water
Spectrum separation is collected 70% ethyl alcohol and is washed successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 60%, 70%, 80%, 95%
De- position, then separated through silica gel column chromatography, successively use volume ratio 85:1,65:1,45:1,25:1,16:1,4:1 chloroform-methanol
Elution collects 16:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg Paris polyphylla is extracted using 8L solvent, 1g medicinal extract 7mL water is dissolved.
Embodiment 3
A method of Rhizoma Paridis extract quickly being prepared, is included the following steps：It is 70% by Paris polyphylla pulverizing medicinal materials volume fraction
Ethyl alcohol extracts 5 times in Microwave Extraction Apparatus, extracts 40min every time, and microwave temperature is 55 DEG C, microwave power 650W, extracts knot
It is filtered after beam, extracting solution is concentrated under reduced pressure to give medicinal extract；AB-8 type large pore resin absorption column color is used after medicinal extract is dissolved with suitable quantity of water
Spectrum separation is collected 70% ethyl alcohol and is washed successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 60%, 70%, 80%, 95%
De- position, then separated through silica gel column chromatography, successively use volume ratio 85:1,65:1,45:1,25:1,16:1,4:1 chloroform-methanol
Elution collects 16:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg Paris polyphylla is extracted using 10L solvent, 1g medicinal extract 9mL water is dissolved.
Microwave extract method provided by the invention is simple to operation, substantially reduces extraction time, simplifies extraction process,
The Paris polyphylla effective part extract of preparation have the function of hemostasis, it is anti-inflammatory, can be prepared on a large scale.
Claims (8)
1. a kind of method for quickly preparing Rhizoma Paridis extract, it is characterised in that include the following steps：By Paris polyphylla pulverizing medicinal materials body
Fraction is that the ethyl alcohol of 60-70% extracts 3-5 times in Microwave Extraction Apparatus, extracts 30-40min, microwave temperature 45-55 every time
DEG C, microwave power 550-650W is filtered after extraction, and extracting solution is concentrated under reduced pressure to give medicinal extract；By medicinal extract with appropriate water-soluble
Large pore resin absorption column chromatographic isolation is used after solution, successively with pure water, the ethyl alcohol of volume fraction 10%, 30%, 60%, 70%, 80%, 95%
Gradient elution is collected 70% alcohol elution, then is separated through silica gel column chromatography, and volume ratio 85 is successively used:1,65:1,45:1,
25:1,16:1,4:1 chloroform-methanol elution, collects 16:1 elution fraction, is concentrated to obtain the final product.
2. according to the method described in claim 1, it is characterized in that：The ethyl alcohol for being 65% by Paris polyphylla pulverizing medicinal materials volume fraction
It is extracted 4 times in Microwave Extraction Apparatus.
3. according to the method described in claim 2, it is characterized in that：Extraction 35min every time.
4. according to the method described in claim 1, it is characterized in that：Microwave temperature is 50 DEG C.
5. according to the method described in claim 1, it is characterized in that：Microwave power is 600W.
6. according to the method described in claim 1, it is characterized in that：1kg Paris polyphylla is extracted using 8-10L solvent.
7. according to the method described in claim 1, it is characterized in that：1g medicinal extract 7-9mL water dissolves.
8. according to the method described in claim 1, it is characterized in that：The large pore resin absorption column is AB-8 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810836558.1A CN108904675A (en) | 2018-07-26 | 2018-07-26 | A method of quickly preparing Rhizoma Paridis extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810836558.1A CN108904675A (en) | 2018-07-26 | 2018-07-26 | A method of quickly preparing Rhizoma Paridis extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108904675A true CN108904675A (en) | 2018-11-30 |
Family
ID=64416830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810836558.1A Withdrawn CN108904675A (en) | 2018-07-26 | 2018-07-26 | A method of quickly preparing Rhizoma Paridis extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108904675A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113730317A (en) * | 2021-09-14 | 2021-12-03 | 浙江宜格企业管理集团有限公司 | Method for extracting effective ingredients for cosmetics from paris polyphylla |
| CN118490600A (en) * | 2024-07-09 | 2024-08-16 | 云南西草资源开发有限公司 | Preparation method and application of paris polyphylla extract |
-
2018
- 2018-07-26 CN CN201810836558.1A patent/CN108904675A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113730317A (en) * | 2021-09-14 | 2021-12-03 | 浙江宜格企业管理集团有限公司 | Method for extracting effective ingredients for cosmetics from paris polyphylla |
| CN113730317B (en) * | 2021-09-14 | 2024-03-26 | 浙江宜格企业管理集团有限公司 | Method for extracting effective components for cosmetics from paris polyphylla |
| CN118490600A (en) * | 2024-07-09 | 2024-08-16 | 云南西草资源开发有限公司 | Preparation method and application of paris polyphylla extract |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108619202A (en) | The method that a kind of extraction of eutectic solvent, macroporous absorbent resin isolate and purify burdock leaf flavonoids | |
| CN104306908A (en) | Health-care composition for preventing respiratory system diseases caused by haze, and preparation method thereof | |
| CN102178730B (en) | Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof | |
| CN108904675A (en) | A method of quickly preparing Rhizoma Paridis extract | |
| CN108704007A (en) | A kind of preparation method of Radix Polygalae active ingredient | |
| KR20110087808A (en) | Prevention of the obesity which contains the complex herb medicine extract (mdt), development of the therapeutic material and the composition development which treats constitutional by liver functional improvements and triglyceride decreases simultaneously | |
| CN105982970A (en) | Traditional Chinese medicinal composition for treating psoriasis and preparation method of traditional Chinese medicinal composition | |
| CN109239239B (en) | Traditional Chinese medicine composition for clearing lung, eliminating phlegm, relieving cough and asthma and preparation method and application thereof | |
| CN101703554B (en) | Preparation and use of semen cuscutae flavonoids | |
| CN107412462B (en) | Preparation method of Huanglong cough and asthma capsule | |
| CN108743657A (en) | The preparation method of methods of glycosides in a kind of mussot swertia herb | |
| CN108379317A (en) | The preparation method of ter penoids in a kind of day name essence | |
| CN107952036B (en) | Traditional Chinese medicine preparation for reducing blood sugar and preparation method thereof | |
| CN1785245A (en) | Method for preparing composite traditional Chinese medicine soft capsule | |
| CN101167798B (en) | Method for preparing active parts in scutellaria decoction for treating ulcer colonitis and uses | |
| CN100506219C (en) | Notoginseng hemostatic and its preparing method | |
| CN104771601B (en) | A kind of combination agent for improving blood viscosity and hematoblastic aggregation | |
| CN103690834B (en) | A kind of blood pressure lowering Chinese medicinal composition and preparation method thereof | |
| CN109718283B (en) | Traditional Chinese medicine composition for relieving asthma and reducing phlegm and preparation method thereof | |
| CN103735713B (en) | A kind of Chinese medicine composition improving nutritional anemia and preparation method thereof | |
| CN102935128B (en) | Preparation method for heat-clearing acne capsule | |
| CN107569462B (en) | Preparation method of Qinchuan Tongbi tablet | |
| CN105902772B (en) | Process for purifying effective components of paris polyphylla, pseudo-ginseng and isatis leaf extract | |
| CN107854651B (en) | Chinese medicinal preparation for treating apoplexy, hyperlipemia and blood hyperviscosity and production method thereof | |
| CN101167830A (en) | Effective part composition for preventing ulcer colonitis and its preparation and use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181130 |