CN108685975A - A kind of preparation method of emblic anti-inflammatory component - Google Patents
A kind of preparation method of emblic anti-inflammatory component Download PDFInfo
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- CN108685975A CN108685975A CN201810772527.4A CN201810772527A CN108685975A CN 108685975 A CN108685975 A CN 108685975A CN 201810772527 A CN201810772527 A CN 201810772527A CN 108685975 A CN108685975 A CN 108685975A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation methods of emblic anti-inflammatory component, and steps are as follows:Phyllanfhus Fruit 60-70% EtOH Sonicates are extracted 2-4 times, each 35-45min, power 240-260W, extracting solution is merged, extracting solution is concentrated under reduced pressure to give medicinal extract;Medicinal extract is used into petroleum ether, chloroform, ethyl acetate and water saturated extracting n-butyl alcohol successively, is concentrated under reduced pressure;It takes chloroform portion to be detached with polyamide column chromatography, uses water, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol elution successively, collect 60% ethanol eluate, be concentrated under reduced pressure, then detached through silica gel column chromatography, use volume ratio 90 successively:1,70:1,55:1,30:1,15:1,1:1 methylene chloride-methanol elution, collects 15:1 elution fraction, concentrates to obtain the final product.The preparation method is easy to operate, can quickly obtain a large amount of anti-inflammatory components, can be prepared applied to drug as the active component of emblic.
Description
Technical field
The present invention relates to a kind of preparation methods of emblic anti-inflammatory component.
Background technology
Emblic also known as mountain phyllanthus emblica, Phyllanthus embical fruit, Phyllanthus embical fruit etc. are Euphorbiaceae phyllanthus plant emblicPhyllanthus emblica L. dry mature fruit.Civil, emblic is usually used in treating disease of biliary tract, bronchus
Inflammation, diabetes etc..Emblic is a kind of with higher edible and medical value wild plant resource, unique flavor, and nutrition is rich
Richness, healthcare function is strong, and the active material beneficial to human body containing there are many has the health-care effects such as help, antitumor, anti-aging,
One of the three kinds of health plants planted in world-wide deployment are specified for the United Nations's health organization.Research has shown that at present, emblic master
To contain phenols, flavones ingredient, there are the multiple efficacies such as Anti-HBV activity, anticancer, antiviral, lipid-loweringing, anti-oxidant, liver protection.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of emblic anti-inflammatory component.
The present invention is realized by following technical solution:
A kind of preparation method of emblic anti-inflammatory component, includes the following steps:By Phyllanfhus Fruit with 60-70% ethyl alcohol in 25-35
Ultrasonic extraction 2-4 times at DEG C, each 35-45min, power 240-260W merge extracting solution, and extracting solution is concentrated under reduced pressure to give leaching
Cream;By medicinal extract to use petroleum ether, chloroform, ethyl acetate and water saturated extracting n-butyl alcohol, decompression dense after appropriate water dissolution successively
Contracting;It takes chloroform portion to be detached with polyamide column chromatography, uses deionized water, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol elution successively, receive
Collect 60% ethanol eluate, be concentrated under reduced pressure, then detached through silica gel column chromatography, uses volume ratio 90 successively:1,70:1,55:1,30:1,
15:1,1:1 methylene chloride-methanol elution, collects 15:1 elution fraction, concentrates to obtain the final product.
Preferably, in the preparation method, by Phyllanfhus Fruit ultrasonic extraction 3 times at 30 DEG C with 65% ethyl alcohol.
Preferably, in the preparation method, each 40min.
Preferably, in the preparation method, power 250W.
Preferably, in the preparation method, 1kg emblics are extracted using 6-8L solvents.
The technology of the present invention effect:
Preparation method provided by the invention is easy to operate, can quickly obtain a large amount of anti-inflammatory components, can be as the effective of emblic
Position is prepared applied to drug.
Specific implementation mode
The essentiality content of the present invention is specifically introduced with reference to embodiment.
Embodiment 1
A kind of preparation method of emblic anti-inflammatory component, includes the following steps:Phyllanfhus Fruit is surpassed with 65% ethyl alcohol at 30 DEG C
Sound extracts 3 times, each 40min, power 250W, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract;By medicinal extract with appropriate
Petroleum ether, chloroform, ethyl acetate and water saturated extracting n-butyl alcohol are used after water dissolution successively, is concentrated under reduced pressure;Chloroform portion is taken to use
Polyamide column chromatography detaches, and uses deionized water, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol elution successively, collects 60% ethanol elution
Liquid is concentrated under reduced pressure, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,70:1,55:1,30:1,15:1,1:1 dichloro
Methane-methanol elutes, and collects 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg emblics are extracted using 7L solvents.
Embodiment 2
A kind of preparation method of emblic anti-inflammatory component, includes the following steps:Phyllanfhus Fruit is surpassed with 60% ethyl alcohol at 25 DEG C
Sound extracts 2 times, each 35min, power 240W, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract;By medicinal extract with appropriate
Petroleum ether, chloroform, ethyl acetate and water saturated extracting n-butyl alcohol are used after water dissolution successively, is concentrated under reduced pressure;Chloroform portion is taken to use
Polyamide column chromatography detaches, and uses deionized water, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol elution successively, collects 60% ethanol elution
Liquid is concentrated under reduced pressure, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,70:1,55:1,30:1,15:1,1:1 dichloro
Methane-methanol elutes, and collects 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg emblics are extracted using 6L solvents.
Embodiment 3
A kind of preparation method of emblic anti-inflammatory component, includes the following steps:Phyllanfhus Fruit is surpassed with 70% ethyl alcohol at 35 DEG C
Sound extracts 4 times, each 45min, power 260W, merges extracting solution, and extracting solution is concentrated under reduced pressure to give medicinal extract;By medicinal extract with appropriate
Petroleum ether, chloroform, ethyl acetate and water saturated extracting n-butyl alcohol are used after water dissolution successively, is concentrated under reduced pressure;Chloroform portion is taken to use
Polyamide column chromatography detaches, and uses deionized water, 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol elution successively, collects 60% ethanol elution
Liquid is concentrated under reduced pressure, then is detached through silica gel column chromatography, uses volume ratio 90 successively:1,70:1,55:1,30:1,15:1,1:1 dichloro
Methane-methanol elutes, and collects 15:1 elution fraction, concentrates to obtain the final product.
Wherein, 1kg emblics are extracted using 8L solvents.
Preparation method provided by the invention is easy to operate, can quickly obtain a large amount of anti-inflammatory components, can be as emblic
Active component is prepared applied to drug.
Claims (5)
1. a kind of preparation method of emblic anti-inflammatory component, it is characterised in that include the following steps:By Phyllanfhus Fruit 60-
Ultrasonic extraction 2-4 times at 25-35 DEG C of 70% ethyl alcohol, each 35-45min, power 240-260W merge extracting solution, extracting solution
It is concentrated under reduced pressure to give medicinal extract;By medicinal extract to use petroleum ether, chloroform, ethyl acetate and water saturated positive fourth after appropriate water dissolution successively
Alcohol extracts, and is concentrated under reduced pressure;Take chloroform portion to be detached with polyamide column chromatography, successively use deionized water, 20% ethyl alcohol, 40% ethyl alcohol,
60% ethanol elution collects 60% ethanol eluate, is concentrated under reduced pressure, then detached through silica gel column chromatography, uses volume ratio 90 successively:1,
70:1,55:1,30:1,15:1,1:1 methylene chloride-methanol elution, collects 15:1 elution fraction, concentrates to obtain the final product.
2. preparation method according to claim 1, it is characterised in that:Phyllanfhus Fruit is surpassed with 65% ethyl alcohol at 30 DEG C
Sound extracts 3 times.
3. preparation method according to claim 2, it is characterised in that:Each 40min.
4. preparation method according to claim 1, it is characterised in that:Power is 250W.
5. preparation method according to claim 1, it is characterised in that:1kg emblics are extracted using 6-8L solvents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810772527.4A CN108685975A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of emblic anti-inflammatory component |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810772527.4A CN108685975A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of emblic anti-inflammatory component |
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| Publication Number | Publication Date |
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| CN108685975A true CN108685975A (en) | 2018-10-23 |
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| CN201810772527.4A Withdrawn CN108685975A (en) | 2018-07-13 | 2018-07-13 | A kind of preparation method of emblic anti-inflammatory component |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110613131A (en) * | 2019-06-20 | 2019-12-27 | 中国林业科学研究院资源昆虫研究所 | Application of emblic leafflower fruit extract in preparing health attenuated food |
-
2018
- 2018-07-13 CN CN201810772527.4A patent/CN108685975A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110613131A (en) * | 2019-06-20 | 2019-12-27 | 中国林业科学研究院资源昆虫研究所 | Application of emblic leafflower fruit extract in preparing health attenuated food |
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Application publication date: 20181023 |
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| WW01 | Invention patent application withdrawn after publication |