CN108362539A - A kind of improvement haematoxylin dyeing liquid and preparation method thereof - Google Patents
A kind of improvement haematoxylin dyeing liquid and preparation method thereof Download PDFInfo
- Publication number
- CN108362539A CN108362539A CN201810228374.7A CN201810228374A CN108362539A CN 108362539 A CN108362539 A CN 108362539A CN 201810228374 A CN201810228374 A CN 201810228374A CN 108362539 A CN108362539 A CN 108362539A
- Authority
- CN
- China
- Prior art keywords
- alum
- haematoxylin
- improvement
- dyeing liquid
- haematoxylin dyeing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 title claims abstract description 67
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 238000004043 dyeing Methods 0.000 title claims abstract description 56
- 239000007788 liquid Substances 0.000 title claims abstract description 54
- 230000006872 improvement Effects 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 40
- 229940037003 alum Drugs 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 229960000583 acetic acid Drugs 0.000 claims abstract description 20
- 239000012153 distilled water Substances 0.000 claims abstract description 20
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 20
- 229910000474 mercury oxide Inorganic materials 0.000 claims abstract description 20
- UKWHYYKOEPRTIC-UHFFFAOYSA-N mercury(ii) oxide Chemical compound [Hg]=O UKWHYYKOEPRTIC-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 239000012074 organic phase Substances 0.000 claims description 6
- 239000012071 phase Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 4
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims 2
- 238000010186 staining Methods 0.000 abstract description 8
- 230000001575 pathological effect Effects 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000003325 follicular Effects 0.000 description 6
- 230000003902 lesion Effects 0.000 description 6
- 210000001685 thyroid gland Anatomy 0.000 description 6
- 230000002380 cytological effect Effects 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000004040 coloring Methods 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- HLUCICHZHWJHLL-UHFFFAOYSA-N Haematein Natural products C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 description 1
- 241001062009 Indigofera Species 0.000 description 1
- 229930182559 Natural dye Natural products 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 208000006374 Uterine Cervicitis Diseases 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 206010008323 cervicitis Diseases 0.000 description 1
- HNNSUZPWERIYIL-UHFFFAOYSA-N chembl1730100 Chemical compound O1CC2(O)CC3=CC(O)=C(O)C=C3C2=C2C1=C(O)C(=O)C=C2 HNNSUZPWERIYIL-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000978 natural dye Substances 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N o-dimethylbenzene Natural products CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 208000008423 pleurisy Diseases 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 238000011121 vaginal smear Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of improvement haematoxylin dyeing liquid and preparation method thereof, belong to biotechnology.The improvement haematoxylin dyeing liquid of the present invention is used as pap staining liquid core dye liquor, composed of the following components：Haematoxylin（C16H14O6）, absolute ethyl alcohol（C2H6O）, alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）Or mixture, the distilled water of two kinds of compounds（H2O）, mercury oxide（HgO）, glacial acetic acid（C2H4O2）.Observation of the improvement haematoxylin dyeing liquid provided by the invention as pap staining liquid core dye liquor for clinically pathological section judges, the nucleus of cell to be checked is in navy blue or darkviolet, kernel takes on a red color, it clinically can multi-faceted observation coloration result, judge the pathologic condition of slice so that diagnostic result is more accurate.
Description
Technical field
The present invention relates to biotechnologies, it particularly relates to a kind of improvement haematoxylin dyeing liquid and preparation method thereof.
Background technology
The diseases such as pneumonia, cervicitis, pleurisy, urinary tract infections are common disease, the frequently-occurring disease of China clinically, these diseases
Sick incidence is high, and healing time is long, how to diagnose these diseases as the hot spot that makes internal disorder or usurp is ground.ThinPrep cytology test can assist facing
Bed doctor has found and formulates correct therapeutic scheme ahead of time to above-mentioned disease, makes patient's rehabilitation as early as possible, and reduction is painful, thus by
Grind the concern of the personnel of making internal disorder or usurp and clinician.One of important step of ThinPrep cytology test is exactly to be dyed to cell.
Papanicolaou's vaginal smear technique is best most common colouring method in pathological section and cast-off cells dyeing, and the decoration method is not
But has and show the features such as nuclear structures are clear, and color separation is apparent, and transparency is good, and endochylema is bright-coloured by color, and contaminated sample is not
Easily decoloration, can preserve for a long time, be usually used in the dye of pathological section sample because that can show the different angling degree of squamous cell
Color.
Haematoxylin is a kind of natural dye, becomes haematein by oxidation, is used for usually as pap staining liquid core dye liquor
The dyeing of biological tissue section nucleus.Traditional pap staining liquid preparation is complex, and staining procedure is complicated and the time is longer,
Make reviewer be difficult to operate, is not easy to promote in different medical unit；In addition traditional pap staining liquid is easily outmoded, and dyes effect
Fruit is unstable, influences the accuracy of the pathology diagosis of doctor, influences the diagnosis of disease.
Invention content
For above-mentioned technical problem in the related technology, a kind of improvement haematoxylin dyeing liquid of present invention proposition and its preparation side
Method can dye nucleus, and the nucleus of cell to be checked is in navy blue or darkviolet, and kernel takes on a red color.Clinically may be used
With multi-faceted observation coloration result, the pathologic condition of slice is judged so that diagnostic result is more accurate.
To realize the above-mentioned technical purpose, the technical proposal of the invention is realized in this way：
A kind of improvement haematoxylin dyeing liquid is used for the dyeing of cell pathology slice as pap staining liquid core dye liquor.
The improvement haematoxylin dyeing liquid includes following components:Haematoxylin（C16H14O6）, absolute ethyl alcohol（C2H6O）, sulfuric acid
Aluminium potassium（KAl(SO4)2）Or alum（NH4Al(SO4)2）Or mixture, the distilled water of two kinds of compounds（H2O）, mercury oxide
（HgO）, glacial acetic acid（C2H4O2）.
Further, the improvement haematoxylin dyeing liquid each component quality:
Haematoxylin（C16H14O6） 2.0〜2.5g
95% absolute ethyl alcohol（C2H6O） 20〜25g
Alum（KAl(SO4)2）Or
Alum（NH4Al(SO4)2） 32〜35g
Distilled water（H2O） 450〜500g
Mercury oxide（HgO） 1.20〜1.25g
Glacial acetic acid（C2H4O2） 8〜10g.
Further, improvement haematoxylin dyeing liquid each component quality composition includes:It is haematoxylin 2.0g, 95% anhydrous
Ethyl alcohol 20g, alum or alum 32g, distilled water 450g, mercury oxide 1.20g, glacial acetic acid 8g.
Further, improvement haematoxylin dyeing liquid each component quality composition includes:It is haematoxylin 2.2g, 95% anhydrous
Ethyl alcohol 22g, alum or alum 33g, distilled water 460g, mercury oxide 1.20g, glacial acetic acid 8g.
Further, improvement haematoxylin dyeing liquid each component quality composition includes:It is haematoxylin 2.4g, 95% anhydrous
Ethyl alcohol 24g, alum or alum 34g, distilled water 480g, mercury oxide 1.22g, glacial acetic acid 8g.
Further, improvement haematoxylin dyeing liquid each component quality composition includes:It is haematoxylin 2.5g, 95% anhydrous
Ethyl alcohol 25g, alum or alum 35g, distilled water 500g, mercury oxide 1.25g, glacial acetic acid 10g.
The preparation method of improvement haematoxylin dyeing liquid includes the following steps：
S1:Each substance is weighed by each component quality proportioning；
S2:Haematoxylin is dissolved in 95% absolute ethyl alcohol, is organic phase；
S3:Or mixtures thereof alum or alum are dissolved in distilled water, are water phase；
S4:After organic phase liquid mixing compatible with water, mercury oxide, glacial acetic acid are added in mixed liquor, stirred and evenly mixed to get improvement
Haematoxylin dyeing liquid.
Beneficial effects of the present invention：
1. preparation method is simple, practical, easy to spread.
2. even dyeing, coloring is stablized.
3. the nucleus of cell to be checked is in navy blue or darkviolet, kernel takes on a red color, easily distinguishable observation.
4. clinically can multi-faceted observation coloration result, judge slice pathologic condition so that diagnostic result more subject to
Really.
Description of the drawings
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the present invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is using the thyroid follicular lesion after the improvement haematoxylin dyeing liquid dyeing described in the embodiment of the present invention 1
Nucleus schematic diagram in cytological samples obtained by fine-needle aspiration.
Fig. 2 is using the thyroid follicular lesion after the improvement haematoxylin dyeing liquid dyeing described in the embodiment of the present invention 2
Nucleus schematic diagram in cytological samples obtained by fine-needle aspiration.
Fig. 3 is using the thyroid follicular lesion after the improvement haematoxylin dyeing liquid dyeing described in the embodiment of the present invention 3
Nucleus schematic diagram in cytological samples obtained by fine-needle aspiration.
Fig. 4 is using the thyroid follicular lesion after the improvement haematoxylin dyeing liquid dyeing described in the embodiment of the present invention 4
Nucleus schematic diagram in cytological samples obtained by fine-needle aspiration.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belong to what the present invention protected
Range.
Embodiment one：
A kind of improvement haematoxylin dyeing liquid, including following components:Haematoxylin（C16H14O6）2.0g, absolute ethyl alcohol（C2H6O）20g
(95%), alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）32g, distilled water（H2O）450g, mercury oxide（HgO）
1.20g, glacial acetic acid（C2H4O2）8g.
Embodiment two：
A kind of improvement haematoxylin dyeing liquid, including following components:Haematoxylin（C16H14O6）2.2g, absolute ethyl alcohol（C2H6O）22g
(95%), alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）33g, distilled water（H2O）460g, mercury oxide（HgO）
1.20g, glacial acetic acid（C2H4O2）8g.
Embodiment three：
A kind of improvement haematoxylin dyeing liquid, including following components:Haematoxylin（C16H14O6）2.4g, absolute ethyl alcohol（C2H6O）24g
(95%), alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）34g, distilled water（H2O）480g, mercury oxide（HgO）
1.22g, glacial acetic acid（C2H4O2）8g.
Example IV：
A kind of improvement haematoxylin dyeing liquid, including following components:Haematoxylin（C16H14O6）2.5g, absolute ethyl alcohol（C2H6O）25g
(95%), alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）35g, distilled water（H2O）500g, mercury oxide（HgO）
1.25g, glacial acetic acid（C2H4O2）10g.
Embodiment five：
Improvement haematoxylin dyeing liquid described in embodiment one to four, preparation method include the following steps：
(1) each substance is weighed by each component quality proportioning；
(2) by haematoxylin（C16H14O6）It is dissolved in absolute ethyl alcohol（C2H6O）, obtain organic phase；
(3) by alum（KAl(SO4)2）Or alum（NH4Al(SO4)2）It is dissolved in distilled water（H2O）, obtain water phase；
(4) organic phase and aqueous phase solution are mixed, obtains mixed liquor；
(5) by mercury oxide（HgO）, glacial acetic acid（C2H4O2）It is added in mixed liquor, stirs and evenly mixs to get improvement haematoxylin dyeing
Liquid.
Embodiment six：
Thyroid follicular lesion cytological samples obtained by fine-needle aspiration is taken, according to the colouring method or tradition of pap staining liquid in the prior art
Cylinder decoration method carry out stained preparation.
Steps are as follows：Alcohol fixation --- clear water flushing --- improvement haematoxylin dyeing liquid dyeing --- clear water flushing ---
--- clear water flushing --- buck (buck is the Lithium carbonate solution or ammonia spirit of saturation) returns indigo plant --- is clear for hydrochloric acid, alcohol differentiation
Water rinses, and --- --- --- washes of absolute alcohol --- dimethylbenzene cleaning --- seals 95% alcohol washes 85% alcohol washes
Piece --- microscopy is observed.
Test effect：
Improvement haematoxylin dyeing liquid of the present invention, being tested through tissue section strain proves, cell dyeing is clear, nucleus knot
Structure is clear, is easy to observe.
The thyroid follicular lesion Fine-needle puncture cell handled using the improvement haematoxylin dyeing liquid of embodiment one to four,
As shown in Figs 1-4, nuclear targeting is clear in structure, and nucleus is in navy blue or darkviolet, and kernel takes on a red color, easily distinguishable sight
It examines.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without
It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (9)
1. a kind of improvement haematoxylin dyeing liquid, which is characterized in that including following components:Haematoxylin, absolute ethyl alcohol, aluminum sulfate salt,
Distilled water, mercury oxide, glacial acetic acid.
2. improvement haematoxylin dyeing liquid according to claim 1, which is characterized in that the aluminum sulfate salt is alum
Or the mixture of alum or two kinds of compounds.
3. improvement haematoxylin dyeing liquid according to claim 1, which is characterized in that the improvement haematoxylin dyeing liquid
Each component quality forms:
2.0 2.5g of haematoxylin
95% absolute ethyl alcohol, 20 25g
Alum or
32 35g of alum
450 500g of distilled water
1.20 1.25g of mercury oxide
8 10g of glacial acetic acid.
4. improvement haematoxylin dyeing liquid according to claim 3, which is characterized in that in the improvement haematoxylin dyeing liquid
The mass ratio of haematoxylin and 95% absolute ethyl alcohol is 1:10.
5. improvement haematoxylin dyeing liquid according to claim 3, which is characterized in that the improvement haematoxylin dyeing liquid is each
Constituent mass forms:Haematoxylin 2.0g, 95% absolute ethyl alcohol 20g, alum or alum 32g, distilled water 450g,
Mercury oxide 1.20g, glacial acetic acid 8g.
6. improvement haematoxylin dyeing liquid according to claim 3, which is characterized in that the improvement haematoxylin dyeing liquid is each
Constituent mass forms:Haematoxylin 2.2g, 95% absolute ethyl alcohol 22g, alum or alum 33g, distilled water 460g,
Mercury oxide 1.20g, glacial acetic acid 8g.
7. improvement haematoxylin dyeing liquid according to claim 3, which is characterized in that the improvement haematoxylin dyeing liquid is each
Constituent mass forms:Haematoxylin 2.4g, 95% absolute ethyl alcohol 24g, alum or alum 34g, distilled water 480g,
Mercury oxide 1.22g, glacial acetic acid 8g.
8. improvement haematoxylin dyeing liquid according to claim 3, which is characterized in that the improvement haematoxylin dyeing liquid is each
Constituent mass forms:Haematoxylin 2.5g, 95% absolute ethyl alcohol 25g, alum or alum 35g, distilled water 500g,
Mercury oxide 1.25g, glacial acetic acid 10g.
9. the preparation method of the improvement haematoxylin dyeing liquid according to claim 1-8, it is characterised in that:The improvement bush
The preparation of plain dyeing liquor includes the following steps：
S1:Each substance is weighed by each component quality proportioning；
S2:Haematoxylin is dissolved in 95% absolute ethyl alcohol, is organic phase；
S3:Or mixtures thereof alum or alum are dissolved in distilled water, are water phase；
S4:After organic phase liquid mixing compatible with water, mercury oxide, glacial acetic acid are added in mixed liquor, stirred and evenly mixed to get improvement
Haematoxylin dyeing liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810228374.7A CN108362539A (en) | 2018-03-20 | 2018-03-20 | A kind of improvement haematoxylin dyeing liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810228374.7A CN108362539A (en) | 2018-03-20 | 2018-03-20 | A kind of improvement haematoxylin dyeing liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108362539A true CN108362539A (en) | 2018-08-03 |
Family
ID=63000578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810228374.7A Pending CN108362539A (en) | 2018-03-20 | 2018-03-20 | A kind of improvement haematoxylin dyeing liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108362539A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102167914A (en) * | 2011-01-21 | 2011-08-31 | 哈尔滨格林标本技术开发有限公司 | Mercury-free environment-friendly hematoxylin staining solution |
| CN102876081A (en) * | 2012-09-24 | 2013-01-16 | 广州鸿琪光学仪器科技有限公司 | Hematoxylin staining solution and papanicolaou staining kit containing same |
| CN103694732A (en) * | 2013-12-31 | 2014-04-02 | 江苏省原子医学研究所 | Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution |
| CN103725040A (en) * | 2013-12-31 | 2014-04-16 | 江苏省原子医学研究所 | Hematoxylin eosin staining solution and preparation method thereof |
| CN105651580A (en) * | 2015-12-28 | 2016-06-08 | 北京雷根生物技术有限公司 | Hematoxylin-eosin mixed staining solution |
| CN107490511A (en) * | 2017-07-25 | 2017-12-19 | 四川金域医学检验中心有限公司 | A kind of haematoxylin dyeing liquid and HE colouring methods |
-
2018
- 2018-03-20 CN CN201810228374.7A patent/CN108362539A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102167914A (en) * | 2011-01-21 | 2011-08-31 | 哈尔滨格林标本技术开发有限公司 | Mercury-free environment-friendly hematoxylin staining solution |
| CN102876081A (en) * | 2012-09-24 | 2013-01-16 | 广州鸿琪光学仪器科技有限公司 | Hematoxylin staining solution and papanicolaou staining kit containing same |
| CN103694732A (en) * | 2013-12-31 | 2014-04-02 | 江苏省原子医学研究所 | Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution |
| CN103725040A (en) * | 2013-12-31 | 2014-04-16 | 江苏省原子医学研究所 | Hematoxylin eosin staining solution and preparation method thereof |
| CN105651580A (en) * | 2015-12-28 | 2016-06-08 | 北京雷根生物技术有限公司 | Hematoxylin-eosin mixed staining solution |
| CN107490511A (en) * | 2017-07-25 | 2017-12-19 | 四川金域医学检验中心有限公司 | A kind of haematoxylin dyeing liquid and HE colouring methods |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105755099A (en) | Papanicolaou staining solution and preparation method thereof | |
| CN106644656B (en) | One step decoration method of Hematoxylin-eosin | |
| CN105651580B (en) | A kind of dyed blended liquid in haematoxylin Yihong | |
| CN103033409B (en) | The histocyte colouring method improved and application thereof | |
| CN110308031A (en) | A kind of stable fungi fluorescent staining liquid | |
| CN106198470A (en) | A kind of fungal detection fluorescence staining liquid and application | |
| CN105950700A (en) | Fungus fluorescent staining agent | |
| CN109187149A (en) | Pap staining kit and its colouring method | |
| CN107238524A (en) | A kind of component of modified form specific stain diagnosticum and preparation method thereof | |
| CN109612807A (en) | A kind of urinary formed element dyeing liquor | |
| CN109387416A (en) | A kind of pap staining liquid for film-making dyeing all-in-one machine | |
| CN102243226A (en) | Improved periodic acid Schiff reaction rapid staining kit | |
| CN108362539A (en) | A kind of improvement haematoxylin dyeing liquid and preparation method thereof | |
| CN110495445A (en) | A kind of method of the Yellow River carp postlarva intermuscular bone dyeing | |
| CN103558150B (en) | A kind of preparation method improveing Pap smear | |
| Elbadawi | Hexachrome modification of Movat's stain | |
| CN107271246B (en) | A kind of immune p16INK4a antigens for Thinprep pap test preserve liquid and preparation method thereof | |
| CN101897328B (en) | Method for staining protozoon sample | |
| CN107300495A (en) | A kind of colouring method of pyriform worm blood film compound stain solution and blood film | |
| CN105571922A (en) | Special dyeing reagent for epithelial tissue diseased cells and preparation process of special dyeing reagent | |
| CN109946140A (en) | Fungi dyeing liquor and fungi colouring method | |
| JP2004502704A (en) | Methylene blue diagnostic agent and diagnostic method for detecting epithelial cancer | |
| CN109425525A (en) | A kind of H-E dyeing liquor for liquid based cytology experiment | |
| CN107271245A (en) | A kind of colouring method of swimming crab flesh spore worm | |
| CN106344934A (en) | Application of fluorescent chemicals in alpha-beta plaque imaging agents |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180803 |
|
| RJ01 | Rejection of invention patent application after publication |