CN107271245A - A kind of colouring method of swimming crab flesh spore worm - Google Patents
A kind of colouring method of swimming crab flesh spore worm Download PDFInfo
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- CN107271245A CN107271245A CN201710432514.8A CN201710432514A CN107271245A CN 107271245 A CN107271245 A CN 107271245A CN 201710432514 A CN201710432514 A CN 201710432514A CN 107271245 A CN107271245 A CN 107271245A
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- 241001533364 Portunus trituberculatus Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000004040 coloring Methods 0.000 title claims abstract description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 91
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000004043 dyeing Methods 0.000 claims abstract description 11
- RZUBARUFLYGOGC-MTHOTQAESA-L acid fuchsin Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C\2C=C(C(=[NH2+])C=C/2)S([O-])(=O)=O)\C=2C=C(C(N)=CC=2)S([O-])(=O)=O)=C1 RZUBARUFLYGOGC-MTHOTQAESA-L 0.000 claims abstract description 10
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 claims abstract description 9
- 239000000834 fixative Substances 0.000 claims abstract description 5
- 239000012188 paraffin wax Substances 0.000 claims abstract description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 25
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 15
- 239000012153 distilled water Substances 0.000 claims description 13
- 239000000975 dye Substances 0.000 claims description 13
- 229960000583 acetic acid Drugs 0.000 claims description 12
- 239000012362 glacial acetic acid Substances 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 230000004069 differentiation Effects 0.000 claims description 7
- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 3
- 241001062009 Indigofera Species 0.000 claims description 2
- HLUCICHZHWJHLL-UHFFFAOYSA-N hematein Chemical compound C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 claims description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims 2
- 239000011260 aqueous acid Substances 0.000 claims 1
- AMWVZPDSWLOFKA-UHFFFAOYSA-N phosphanylidynemolybdenum Chemical compound [Mo]#P AMWVZPDSWLOFKA-UHFFFAOYSA-N 0.000 claims 1
- 210000003205 muscle Anatomy 0.000 abstract description 16
- 230000003902 lesion Effects 0.000 abstract description 9
- 210000001519 tissue Anatomy 0.000 abstract description 8
- 239000000835 fiber Substances 0.000 abstract description 3
- 230000018044 dehydration Effects 0.000 abstract description 2
- 238000006297 dehydration reaction Methods 0.000 abstract description 2
- 230000007935 neutral effect Effects 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 235000011167 hydrochloric acid Nutrition 0.000 description 6
- 229940034610 toothpaste Drugs 0.000 description 5
- 239000000606 toothpaste Substances 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000243190 Microsporidia Species 0.000 description 2
- 208000000260 Warts Diseases 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000024241 parasitism Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 201000010153 skin papilloma Diseases 0.000 description 2
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 description 1
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to biological field, more particularly to a kind of colouring method of swimming crab flesh spore worm, a kind of specifically colouring method for the entozoic swimming crab flesh spore worm of Portunus trituberculatus Miers muscle.The colouring method that the present invention is provided includes：Swimming crab musculature is fixed with Davidson ' s alcoholformalacetic fixatives；Make tissue paraffin embedded block；Dimethylbenzene dewaxes；Ethanol gradient aquation；WeigertShi Garapa uniformly dyeings liquid is dyed；Ponceaux acid fuchsin liquid is dyed；Aniline blue liquid is redyed；Ethanol dehydration, dimethylbenzene is transparent, dries rear neutral gum mounting.After the colouring method provided with the present invention is dyed：After lesion musculature is dyed, muscle fibre is in blue bar banding, and swimming crab flesh spore worm spore takes on a red color oval, and morphosis is clear, clear with background tissues contrast, is easily distinguished.
Description
Technical field
It is specifically a kind of the present invention relates to biological field, more particularly to a kind of colouring method of swimming crab flesh spore worm
For the colouring method of the entozoic swimming crab flesh spore worm of Portunus trituberculatus Miers muscle.
Background technology
Swimming crab flesh spore worm is a kind of microsporidian for infecting Portunus trituberculatus Miers newfound in recent years, and main parasitic is three
The musculature of wart swimming crab, it is in albefaction shape to cause muscle changes.Understand according to investigations, the disease has occurred many in Deposits in Eastern Coastal China
Year, because of the similar toothpaste of muscle that sick crab appendage is extruded, therefore raiser is called " toothpaste disease ".The sick Epidemic Scope is wide, Shandong, river
The coastal regions such as Soviet Union, Zhejiang, Fujian are found, and period of disease is generally 7~November, peak mortality phase in 9~October.Swimming crab
Flesh spore worm is different from the aquatic parasite of other classes, and it has the entozoic feature of special sexual cell, once formed into host
Ripe spore, general exogenous drugs are difficult that it is killed, cultivation middle and later periods swimming crab whole-body muscle because of microsporidian parasitism
Commodity value is lost in albefaction, and clinical symptoms are difficult to be found by raiser, and later stage mortality causes raiser to cultivate income
It is impaired.Swimming crab flesh spore worm has influenceed the cultured output of China's Portunus trituberculatus Miers.
Histopathological examination clear and intuitive can reflect the concrete position of Infected with Pathogenic Fungi, and it is to colonize in carefully to determine cause of disease
Extracellular environment or intracellular, or even it is in nucleus endoparasitism or in cytoplasm endoparasitism that can distinguish cause of disease.Molecule is diagnosed
Although method rapidly develops in recent years, and the extensive use in clinical diagnosis, merely using Molecular tools, such as PCR method, only
It can confirm that whether institute's sampling tissue contains pathogen, and the parasitism and histopathologic change to cause of disease can not directly embody,
Using histopathology means, the simple deficiency using molecular detecting method can be made up.Dyeing is that tissue pathological slice makes
In vital link, preferable and reliable hair colouring methods are conducive to distinguishing and diagnosing cause of disease.HE decoration methods are histopathologies
In the most general colouring method, it is wide that it is applicable biological species scope, has preferable Color to most organ-tissues, because
This, in pathological study and diagnostic application, it is frequently as preferred colouring method.But this method also has limitation, we are to suffering from
The muscle of " toothpaste disease " crab carries out histopathological study discovery, can be with microscopy to substantial amounts of shuttle in the wet mount of lesion muscle
Sub- crab flesh spore worm spore, and in conventional H E stained slices, but it is difficult to which microscopy is to substantial amounts of spore, under high power field
(1000 times), spore coloring is shallower, contrasts unobvious with musculature background color, it is difficult to distinguish.Therefore, for three wart shuttles
Crab " toothpaste disease " is, it is necessary to set up a kind of special colouring method, by spore and the differentiation of lesion musculature.
The content of the invention
It is an object of the invention to provide a kind of colouring method of swimming crab flesh spore worm.The present invention is real by following scheme
Existing：
A kind of colouring method of swimming crab flesh spore worm, comprises the following steps：
S1. swimming crab musculature is taken, is fixed with Davidson ' s alcoholformalacetic fixatives；
S2. tissue paraffin embedded block is made；Then 5um thickness sections are made using histotome；
S3. dimethylbenzene dewaxes 2 times；Time can be 5min/ times；
S4. ethanol gradient aquation：100% ethanol 2-4min, 100% ethanol 2-4min, 95% ethanol 2-4min, 85% second
Alcohol 2-4min, 75% ethanol 2-4min, 50% ethanol 2-4min, distillation washing 2-4min；The concentration of alcohol is volume hundred
Divide ratio；
S5.WeigertShi Garapa uniformly dyeings liquid dyes 4-6min, and washing, 1% hydrochloride alcohol differentiation, flowing water is rinsed and returned
It is blue；The ethanol that the hydrochloric acid that it is 37% with mass fraction that described 1% hydrochloride alcohol, which is, is 70% with volume fraction is by volume 1:99
Mix；
S6. Ponceaux acid fuchsin liquid dyes 6-8min, water rinsing, phosphomolybdic acid aqueous solution differentiation 3-5min；
S7. aniline blue liquid redyes 4-6min, and percent by volume breaks up 1min for 1% glacial acetic acid aqueous solution；
S8. it is dehydrated respectively with the ethanol and 100% ethanol of 95% percent by volume, dimethylbenzene is transparent, dries.
Wherein, WeigertShi haematoxylins dye liquor described in step S5 include solution A and solution B；During dyeing, by solution A and
Solution B is mixed in equal volume；
The preparation method of the solution A is that haematine is pressed into 0.8~1.2g:100ml ratio is dissolved in percent by volume
For 95% alcohol；
The preparation method of the solution B to take the mass percent to be after 29% liquor ferri trichloridi is added in distilled water,
Concentrated hydrochloric acid is added, the volume ratio of the liquor ferri trichloridi, distilled water and concentrated hydrochloric acid is 4：95：1；The quality of the concentrated hydrochloric acid
Percentage is 37%.
Wherein, Ponceaux acid fuchsin dye liquor is pressed by Ponceaux, acid fuchsin, water and glacial acetic acid described in step S6
0.7g：0.3g：99ml：1ml is formulated.
Wherein, the mass percent of the phosphomolybdic acid aqueous solution described in step S6 is 1%.
Wherein, aniline blue liquid described in step S7 presses 2g by aniline blue, water and glacial acetic acid：98ml：2ml is formulated.
After the colouring method provided with the present invention is dyed：After lesion musculature is dyed, muscle fibre is in blue bar
Banding, swimming crab flesh spore worm spore takes on a red color oval, and morphosis is clear, clear with background tissues contrast, easily distinguishes.
Brief description of the drawings
Fig. 1 dyes for lesion musculature HE, 400 times.In figure, 1 is muscle, 2 swimming crab flesh spore worms.
Lesion musculature is dyed in Fig. 2 embodiments 1,400 times.In figure, 1 is muscle, 2 swimming crab flesh spore worms.
Fig. 3 lesion musculatures HE is dyed, 1000 times.In figure, 1 is muscle, 2 swimming crab flesh spore worms.
Lesion musculature is dyed in Fig. 4 embodiments 1,1000 times.In figure, 1 is muscle, 2 swimming crab flesh spore worms.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
Prepare 95% ethanol：Alcohol 95 ml, distilled water 5ml.
Prepare 85% ethanol：Ethanol 85ml, distilled water 15ml.
Prepare 75% ethanol：Ethanol 75ml, distilled water 25ml.
Prepare 50% ethanol：Ethanol 50ml, distilled water 50ml.
Davidson ' s alcoholformalacetic fixatives：Glacial acetic acid 57.5ml, formaldehyde 110ml, 95% ethanol 165ml, filtering sea
167.5ml。
Prepare WeigertShi haematoxylin dye liquors：Solution A：1g haematines are added into the alcohol of 100ml 95%, dissolving.Solution
B：29% liquor ferri trichloridi 4ml is taken, is added in 95ml distilled water, 1ml concentrated hydrochloric acids are eventually adding.During dyeing, by solution A and
B equal proportions are mixed, and two liquid should not be pre-mixed, and otherwise premixed liquid easily aoxidizes and loses dyeing capacity.
The hydrochloride alcohol of preparation 1%：The hydrochloric acid of 1ml 37% (mass fraction), the ethanol of 99ml 70% (volume fraction).
Prepare Ponceaux acid fuchsin dye liquor：Ponceaux 0.7g, acid fuchsin 0.3g, distilled water 99ml, glacial acetic acid 1ml.
Prepare the phosphomolybdic acid aqueous solution：Phosphomolybdic acid 1g, distilled water 99g.
Prepare the aniline blue aqueous solution：Aniline blue 2g, distilled water 98ml, glacial acetic acid 2ml.
Prepare 1% glacial acetic acid：Glacial acetic acid 1ml, distilled water 99ml.
Dyeing course：
1. dissecting live body or dying " toothpaste crab ", clip musculature fixes 24h with Davidson ' s alcoholformalacetic fixatives.
2. conventional method makes tissue paraffin embedded block, then 5um thickness sections are made using histotome.
3. dimethylbenzene dewaxes 2 times, each 5min.
4. ethanol aquation：The ethanol of 100% ethanol 3min, 100% ethanol 3min, 95% 3min, 85% ethanol 3min, 75%
Ethanol 3min, 50% ethanol 3min, distillation washing 3min.
5.WeigertShi Garapa uniformly dyeings liquid dyes 5min, originally washes, 1% hydrochloride alcohol differentiation several seconds, flowing water punching
Wash several minutes and return indigo plant.
6. Ponceaux acid fuchsin liquid dyes 7min, distilled water short rinse, the phosphomolybdic acid aqueous solution breaks up about 3-5min,
7. aniline blue liquid redyes 5min, 1% glacial acetic acid differentiation 1min.
8. 95% ethanol and 100% ethanol dehydration, dimethylbenzene are transparent, rear neutral gum mounting is dried.
Shown in 400 times and 1000 times of figure as Fig. 2 and Fig. 4, wherein 1 is muscle, 2 swimming crab flesh spore worms.Embodiment 1 is dyed
As a result it can be seen that after lesion musculature is dyed, (after dyeing, 1 muscle fibre is in blue bar banding, 2 swimming crab flesh spore worm spores
Son takes on a red color oval) morphosis is clear, clear with background tissues contrast, easily distinguish.And HE Colors are shown in Fig. 1 and Fig. 3
It is shown, wherein 1 is muscle, 2 swimming crab flesh spore worms.
It is described above be presently preferred embodiments of the present invention, but the present invention should not be limited to disclosed in the embodiment
Content.So every do not depart from the lower equivalent or modification completed of spirit disclosed in this invention, the model that the present invention is protected is both fallen within
Enclose.
Claims (5)
1. a kind of colouring method of swimming crab flesh spore worm, it is characterised in that comprise the following steps：
S1. swimming crab musculature is taken, is fixed with Davidson ' s alcoholformalacetic fixatives；
S2. tissue paraffin embedded block is made；
S3. dimethylbenzene dewaxes 2 times；
S4. ethanol gradient aquation：100% ethanol 2-4min, 100% ethanol 2-4min, 95% ethanol 2-4min, 85% ethanol 2-
4min, 75% ethanol 2-4min, 50% ethanol 2-4min, distillation washing 2-4min；The concentration of alcohol is percent by volume；
S5.WeigertShi Garapa uniformly dyeings liquid dyes 4-6min, and washing, 1% hydrochloride alcohol differentiation, flowing water rinses and returns indigo plant；Institute
The ethanol that the hydrochloric acid that it is 37% with mass fraction that the hydrochloride alcohol for stating 1%, which is, is 70% with volume fraction is by volume 1:99 mixing and
Into；
S6. Ponceaux acid fuchsin liquid dyes 6-8min, water rinsing, phosphomolybdic acid aqueous solution differentiation 3-5min；
S7. aniline blue liquid redyes 4-6min, and percent by volume breaks up 1min for 1% glacial acetic acid aqueous solution；
S8. it is dehydrated respectively with the ethanol and 100% ethanol of 95% percent by volume, dimethylbenzene is transparent, dries.
2. the colouring method of the swimming crab flesh spore worm according to claim 1, it is characterised in that described in step S5
WeigertShi haematoxylins dye liquor includes solution A and solution B；During dyeing, solution A and solution B are mixed in equal volume；
The preparation method of the solution A is that haematine is pressed into 0.8~1.2g:100ml ratio is dissolved in percent by volume
95% alcohol；
The preparation method of the solution B is takes the mass percent to be after 29% liquor ferri trichloridi is added in distilled water, then adds
Enter concentrated hydrochloric acid, the volume ratio of the liquor ferri trichloridi, water and concentrated hydrochloric acid is 4：95：1；The mass percent of the concentrated hydrochloric acid is
37%.
3. the colouring method of the swimming crab flesh spore worm according to claim 1, it is characterised in that beautiful spring described in step S6
Red acid fuchsin dye liquor presses 0.7g by Ponceaux, acid fuchsin, water and glacial acetic acid：0.3g：99ml：1ml is formulated.
4. the colouring method of the swimming crab flesh spore worm according to claim 1, it is characterised in that phosphorus molybdenum described in step S6
The mass percent of aqueous acid is 1%.
5. the colouring method of the swimming crab flesh spore worm according to claim 1, it is characterised in that aniline described in step S7
Blue liquid presses 2g by aniline blue, water and glacial acetic acid：98ml：2ml is formulated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432514.8A CN107271245A (en) | 2017-06-09 | 2017-06-09 | A kind of colouring method of swimming crab flesh spore worm |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432514.8A CN107271245A (en) | 2017-06-09 | 2017-06-09 | A kind of colouring method of swimming crab flesh spore worm |
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| Publication Number | Publication Date |
|---|---|
| CN107271245A true CN107271245A (en) | 2017-10-20 |
Family
ID=60065936
Family Applications (1)
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|---|---|---|---|
| CN201710432514.8A Pending CN107271245A (en) | 2017-06-09 | 2017-06-09 | A kind of colouring method of swimming crab flesh spore worm |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108760444A (en) * | 2018-08-01 | 2018-11-06 | 迈克生物股份有限公司 | Staining kit and colouring method for tissue fibers |
| CN109187147A (en) * | 2018-08-27 | 2019-01-11 | 广州医科大学附属第医院 | A kind of dyeing of arterial wall ingredient and identification method |
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| CN103940648A (en) * | 2014-04-04 | 2014-07-23 | 山西农业大学 | Preparation method for gill tissue paraffin section |
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2017
- 2017-06-09 CN CN201710432514.8A patent/CN107271245A/en active Pending
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| CN101897328A (en) * | 2010-01-11 | 2010-12-01 | 中国疾病预防控制中心寄生虫病预防控制所 | Method for staining protozoon sample |
| CN102697581A (en) * | 2012-05-16 | 2012-10-03 | 上海交通大学医学院附属上海儿童医学中心 | Method for constructing tissue engineering blood vessel |
| CN103940648A (en) * | 2014-04-04 | 2014-07-23 | 山西农业大学 | Preparation method for gill tissue paraffin section |
| CN104352508A (en) * | 2014-09-29 | 2015-02-18 | 大连医科大学 | Application of dioscin to preparation of renal injury protection medicament |
| CN106755518A (en) * | 2017-02-15 | 2017-05-31 | 中国水产科学研究院东海水产研究所 | A kind of swimming crab flesh spore worm real time quantitative PCR detecting reagent kit |
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| Title |
|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108760444A (en) * | 2018-08-01 | 2018-11-06 | 迈克生物股份有限公司 | Staining kit and colouring method for tissue fibers |
| CN109187147A (en) * | 2018-08-27 | 2019-01-11 | 广州医科大学附属第医院 | A kind of dyeing of arterial wall ingredient and identification method |
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