CN109387416A - A kind of pap staining liquid for film-making dyeing all-in-one machine - Google Patents
A kind of pap staining liquid for film-making dyeing all-in-one machine Download PDFInfo
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- CN109387416A CN109387416A CN201710680343.0A CN201710680343A CN109387416A CN 109387416 A CN109387416 A CN 109387416A CN 201710680343 A CN201710680343 A CN 201710680343A CN 109387416 A CN109387416 A CN 109387416A
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- Prior art keywords
- dyeing
- staining liquid
- liquid
- pap
- film
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- 239000007788 liquid Substances 0.000 title claims abstract description 45
- 238000004043 dyeing Methods 0.000 title claims abstract description 37
- 238000010186 staining Methods 0.000 title claims abstract description 33
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 28
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims abstract description 28
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229960000583 acetic acid Drugs 0.000 claims abstract description 14
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 13
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 claims abstract description 12
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims abstract description 7
- 229960001506 brilliant green Drugs 0.000 claims abstract description 7
- HXCILVUBKWANLN-UHFFFAOYSA-N brilliant green cation Chemical compound C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 HXCILVUBKWANLN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000011187 glycerol Nutrition 0.000 claims abstract description 7
- NALMPLUMOWIVJC-UHFFFAOYSA-N n,n,4-trimethylbenzeneamine oxide Chemical compound CC1=CC=C([N+](C)(C)[O-])C=C1 NALMPLUMOWIVJC-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940032753 sodium iodate Drugs 0.000 claims abstract description 7
- 235000015281 sodium iodate Nutrition 0.000 claims abstract description 7
- 239000011697 sodium iodate Substances 0.000 claims abstract description 7
- MPVDXIMFBOLMNW-ISLYRVAYSA-N 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid Chemical compound OC1=CC=C2C=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1\N=N\C1=CC=CC=C1 MPVDXIMFBOLMNW-ISLYRVAYSA-N 0.000 claims abstract description 6
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 claims abstract description 5
- -1 Yihong Chemical compound 0.000 claims abstract description 4
- 229960005150 glycerol Drugs 0.000 claims abstract description 4
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims abstract description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 239000008213 purified water Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 2
- UYDPQDSKEDUNKV-UHFFFAOYSA-N phosphanylidynetungsten Chemical compound [W]#P UYDPQDSKEDUNKV-UHFFFAOYSA-N 0.000 claims 1
- 238000009595 pap smear Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 12
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 239000000975 dye Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 2
- 201000010881 cervical cancer Diseases 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000675108 Citrus tangerina Species 0.000 description 1
- 208000005775 Parakeratosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 208000008423 pleurisy Diseases 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of pap staining liquid for film-making dyeing all-in-one machine, is related to biologic product technology field.The pap staining liquid, including improvement haematoxylin dyeing liquid, EA50/OG complex staining liquid.Wherein, improvement haematoxylin dyeing liquid includes following components: sodium iodate, hematoxylin, aluminum aluminum sulfate, dehydrated alcohol, formaldehyde, glacial acetic acid and water.The EA50/OG complex staining liquid includes following components: brilliant green, orange G, Yihong, phosphotungstic acid, methanol, glacial acetic acid, glycerine, dehydrated alcohol and water.Invention reduces use cost, save the time for preparing sample, the pap staining liquid cooperation film-making dyeing all-in-one machine even dyeing of offer, the eucaryotic cell structure after dyeing is clear, cell color is bright-coloured, is easy to the observation judgement of clinically Thinprep pap test film-making.
Description
Technical field
The present invention relates to biologic product technology fields；More particularly to a kind of pap staining for film-making dyeing all-in-one machine
Liquid.
Background technique
Currently, the diseases such as cervix cancer, pulmonary tuberculosis, pleurisy, urinary tract infections become China's common disease clinically, more
The disease incidence of morbidity, these diseases is high, and healing time is long, and the early discovery early treatment of cervix cancer is even more to become rescue patient vitals
The most important thing, how efficient diagnosis these diseases are increasingly becoming the hot spot of pathological research.
ThinPrep cytology test can adjuvant clinical doctor above-mentioned disease is found ahead of time and formulates correct treatment side
Case makes patient's rehabilitation as early as possible, reduces pain, thus the concern by researcher and clinician.
One of important step of ThinPrep cytology test is exactly to dye to cell, and pap staining liquid is most often
One of colouring method.Using pap staining liquid dye sample cell transparency it is good, it is clear in structure, film-making it is rich in color and
It is bright-coloured, it is usually used in the dyeing of gynaecology's sample due to can show the different angling degree of squamous cell.
Most hospitals and physical examination mechanism are all made of film-making dyeing all-in-one machine and replace pure manual smear operation at this stage, from
The instrument of dynamicization has higher requirement to the dyeing effect of pap staining liquid.
But traditional pap staining liquid preparation is complex, and dyeing liquor is easily outmoded, and dyeing effect is unstable, and cooperation is certainly
The high film-making of dynamicization degree is dyed ineffective when all-in-one machine, influences the accuracy of the pathology diagosis of doctor, influences examining for disease
It is disconnected.
Therefore, the present invention is developed on the basis of mainly solving currently available technology and having above insufficient.
Summary of the invention
The problem to be solved in the present invention is that traditional pap staining liquid preparation is complex, and dyeing liquor is easily outmoded, Er Qieran
Color effect is unstable, cooperates the film-making of high degree of automation to dye ineffective when all-in-one machine, influences the pathology diagosis of doctor
Accuracy influences the diagnosis of disease.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: it provides a kind of for film-making dyeing all-in-one machine
Pap staining liquid, belong to field of biotechnology.The pap staining liquid, including improvement haematoxylin dyeing liquid, EA50/OG are compound
Dyeing liquor.Wherein, improvement haematoxylin dyeing liquid includes following components: sodium iodate, hematoxylin, aluminum aluminum sulfate, dehydrated alcohol, first
Aldehyde, glacial acetic acid and water.
Further, every 1000ml improves haematoxylin dyeing liquid, including following components: 0, of sodium iodate, 03~0 .05g；
1~3g of hematoxylin；10~30g of aluminum aluminum sulfate；100~300ml of dehydrated alcohol；Formaldehyde 0.1g；10~30ml of glacial acetic acid；
Surplus is purified water.
Preferably, the EA50 dyeing liquor includes following components: brilliant green, orange G, Yihong, phosphotungstic acid, methanol, glacial acetic acid,
Glycerine, dehydrated alcohol and water.
Further, every 1000mlEA50/OG complex staining liquid, including following components: 0, of brilliant green, 2~0,6g；Orange
Yellow 2~6g of G；1~4g of Yihong；2~6g of phosphotungstic acid；50~200ml of methanol；10~30ml of glacial acetic acid；Glycerine 100~
150ml；50~100ml of purified water；Surplus is dehydrated alcohol.
The beneficial effects of the present invention are:
1, eucaryotic cell structure after pap staining liquid even dyeing provided by the invention, dyeing is clear, cell color is bright-coloured, is easy to face
The observation of Thinprep pap test film-making on bed judges.
2, in pap staining liquid provided by the invention, including improvement haematoxylin dyeing liquid, EA50/OG complex staining liquid, point
The other different parts to cell dye: improvement hematoxylin dyeing liquor dyes nucleus, the cell of cell to be checked
Core is in navy blue or darkviolet, and kernel takes on a red color；EA50/OG complex staining liquid dyes cytoplasm, cell to be checked it is thin
Endochylema display pink or blue-green, the cytoplasm pinkiness of parakeratosis cell, the cytoplasm of complete keratinocyte are in tangerine
Yellow.Two kinds of dyeing liquors cooperate, clinically can multi-faceted observation coloration result, judge the pathologic condition of slice so that
Diagnostic result is more accurate.
3, ingredient is simple, cheap, easy to spread.
4, easy to operate using simple, the time for preparing sample is greatly saved.
Specific embodiment
Below by way of specific embodiment, the present invention is further explained.
It is a kind of for film-making dyeing all-in-one machine pap staining liquid, it is characterised in that: including improvement haematoxylin dyeing liquid,
EA50/OG complex staining liquid.Wherein, improvement haematoxylin dyeing liquid include following components: sodium iodate, hematoxylin, aluminum aluminum sulfate,
Dehydrated alcohol, formaldehyde, glacial acetic acid and water.The EA50/OG complex staining liquid includes following components: brilliant green, orange G, Yihong, phosphorus
Wolframic acid, methanol, glacial acetic acid, glycerine, dehydrated alcohol and water.
Embodiment 1, pap staining liquid making method of the invention are as follows:
Every 1000ml improves haematoxylin dyeing liquid, 0,04g of sodium iodate；Hematoxylin 3g；Aluminum aluminum sulfate 28g；Dehydrated alcohol
260ml；Formaldehyde 0.1g；Glacial acetic acid 27ml；Surplus is purified water, and above-mentioned each component uniform stirring is allowed to dissolve.
Further, every 1000mlEA50/OG complex staining liquid, including following components: 0,6g of brilliant green；Orange G 6g；
Yihong 3g；Phosphotungstic acid 5g；Methanol 170ml；Glacial acetic acid 30ml；Glycerine 110ml；Purified water 80ml；Surplus is anhydrous second
Alcohol allows above-mentioned each component uniform stirring to dissolve.
The pap staining liquid made by above embodiments in use process, reduces use cost, saves system
The time of standby sample, eucaryotic cell structure after the pap staining liquid cooperation film-making dyeing all-in-one machine even dyeing provided, dyeing is clear,
Cell color is bright-coloured, is easy to the observation judgement of clinically Thinprep pap test film-making.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit application field of the present invention.It is all in the present invention
Any modifications, equivalent replacements, and improvements etc. done within spirit and principle, should all be included in the protection scope of the present invention.
Claims (3)
1. it is a kind of for film-making dyeing all-in-one machine pap staining liquid, it is characterised in that: including improvement haematoxylin dyeing liquid,
EA50/OG complex staining liquid, wherein improvement haematoxylin dyeing liquid include following components: sodium iodate, hematoxylin, aluminum aluminum sulfate,
Dehydrated alcohol, formaldehyde, glacial acetic acid and water；EA50/OG complex staining liquid includes following components: brilliant green, orange G, Yihong, phosphorus tungsten
Acid, methanol, glacial acetic acid, glycerine, dehydrated alcohol and water.
2. pap staining liquid according to claim 1, it is characterised in that: every 1000ml improves haematoxylin dyeing liquid, including
With
Lower component: 0, of sodium iodate, 03~0 .05g；1~3g of hematoxylin；10~30g of aluminum aluminum sulfate；Dehydrated alcohol 100
~300ml；Formaldehyde 0.1g；10~30ml of glacial acetic acid；Surplus is purified water.
3. pap staining liquid according to claim 1, it is characterised in that: every 1000mlEA50/OG complex staining liquid, including
Following components: 0, of brilliant green, 2~0,6g；2~6g of orange G；1~4g of Yihong；2~6g of phosphotungstic acid；Methanol 50~
200ml；10~30ml of glacial acetic acid；100~150ml of glycerine；50~100ml of purified water；Surplus is dehydrated alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710680343.0A CN109387416A (en) | 2017-08-10 | 2017-08-10 | A kind of pap staining liquid for film-making dyeing all-in-one machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710680343.0A CN109387416A (en) | 2017-08-10 | 2017-08-10 | A kind of pap staining liquid for film-making dyeing all-in-one machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109387416A true CN109387416A (en) | 2019-02-26 |
Family
ID=65414194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710680343.0A Pending CN109387416A (en) | 2017-08-10 | 2017-08-10 | A kind of pap staining liquid for film-making dyeing all-in-one machine |
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| Country | Link |
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| CN (1) | CN109387416A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006724A (en) * | 2019-04-17 | 2019-07-12 | 郑州安图生物工程股份有限公司 | Trichomonad reagent is detected using Pasteur and Gram-staining process |
| CN111024471A (en) * | 2019-12-17 | 2020-04-17 | 浙江殷欣生物技术有限公司 | Liquid-based cell detection kit for cervical cancer screening and use method |
| CN113945440A (en) * | 2021-10-14 | 2022-01-18 | 山东高创医疗器械国家研究院有限公司 | Papanicolaou staining kit, preparation method thereof and staining method |
| CN114441272A (en) * | 2022-01-27 | 2022-05-06 | 北京艾迪康医学检验实验室有限公司 | Exfoliative cell staining solution and preparation method thereof |
-
2017
- 2017-08-10 CN CN201710680343.0A patent/CN109387416A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006724A (en) * | 2019-04-17 | 2019-07-12 | 郑州安图生物工程股份有限公司 | Trichomonad reagent is detected using Pasteur and Gram-staining process |
| CN110006724B (en) * | 2019-04-17 | 2021-06-04 | 郑州安图生物工程股份有限公司 | Reagent for detecting trichomonas by adopting papanicolaou and gram staining method |
| CN111024471A (en) * | 2019-12-17 | 2020-04-17 | 浙江殷欣生物技术有限公司 | Liquid-based cell detection kit for cervical cancer screening and use method |
| CN113945440A (en) * | 2021-10-14 | 2022-01-18 | 山东高创医疗器械国家研究院有限公司 | Papanicolaou staining kit, preparation method thereof and staining method |
| CN114441272A (en) * | 2022-01-27 | 2022-05-06 | 北京艾迪康医学检验实验室有限公司 | Exfoliative cell staining solution and preparation method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190226 |
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| WD01 | Invention patent application deemed withdrawn after publication |