CN110495445A - A kind of method of the Yellow River carp postlarva intermuscular bone dyeing - Google Patents
A kind of method of the Yellow River carp postlarva intermuscular bone dyeing Download PDFInfo
- Publication number
- CN110495445A CN110495445A CN201910891975.0A CN201910891975A CN110495445A CN 110495445 A CN110495445 A CN 110495445A CN 201910891975 A CN201910891975 A CN 201910891975A CN 110495445 A CN110495445 A CN 110495445A
- Authority
- CN
- China
- Prior art keywords
- postlarva
- dyeing
- yellow river
- minutes
- fish body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
Abstract
The invention discloses a kind of the Yellow River carp postlarva intermuscular bone colouring method, detailed processes are as follows: fixes the Yellow River carp postlarva sample with paraformaldehyde；PFA is washed away with distilled water；Postlarva sample is impregnated with TBST；Remaining TBST is washed away with distilled water；With the transparent the Yellow River carp postlarva sample of KOH solution；The KOH solution more renewed, and Alizarin red staining is added dropwise；Dye liquor is outwelled, distilled water is added and washes away loose colour；The Yellow River carp postlarva is gone in the KOH solution of hydrogen peroxide, surface pigment is removed；Remaining hydrogen peroxide is washed away with KOH solution；It is transparent that the Yellow River carp postlarva is gone into gradient in glycerol；It takes pictures under body formula mirror retention；The Yellow River carp postlarva is gone in 100% glycerol and is saved.The present invention uses optimal reagent, pass through the exploration to different dyeing conditions, optimal dyeing condition is filtered out, it is final to obtain the high-quality sample of the Yellow River carp postlarva that body is transparent, intermuscular bone dyeing is red or purple, intermuscular bone attachment position can be clearly observed with body formula mirror.
Description
Technical field
The invention belongs to postlarva intermuscular bone staining technique fields, and in particular to a kind of side of the Yellow River carp postlarva intermuscular bone dyeing
Method, this method have important use in the Yellow River carp intermuscular bone ossific process and Study on Molecular Mechanism, suitable for obtaining after dyeing
Transparent specimen can be clearly observed the attachment position of postlarva intermuscular bone with stereoscope.
Background technique
The Yellow River carp (Cyprinus carpio) be subordinate to Cypriniformes (Cypriniformes), Cyprinidae (Cyprinidae), carp
Belong to (Cyprinus).The Yellow River carp is the distinctive important economic freshwater fish of the long-term self-assembling formation in China the Yellow River, adaptable
By force, the advantages that feeding habits are wide, resistance is strong, specious, delicious flavour.But due to the presence of intermuscular bone, to the edible of the Yellow River carp
Great inconvenience is brought, the development of the aquaculture of the Yellow River carp is also limited.The dyeing of the Yellow River carp postlarva not only promotes understanding intermuscular bone
Distribution and ossification, moreover it is possible to lay the foundation for the few intermuscular bone fish of research and cultivation of its intermuscular bone molecular mechanism.
The intermuscular bone of fish pierces between being also known as flesh, be by the mesenchymal cell between flesh without cartilage phase direct development and
Come.Three classes can be classified as according to intermuscular bone attachment area: the epineurals being attached on neural arch, the arcus haemalis being attached on arcus haemalis
Ossiculum and it is attached to epicentral centrum ossiculum.
There is the staining technique of megalobrama amblycephala and ricefield eel adult fish at present, but has been contaminated not yet for the Yellow River carp postlarva intermuscular bone
Color method.Megalobrama amblycephala is at fish length generally between 165-456mm, and the body of the Yellow River carp postlarva is grown between 9-15mm, and weight exists
Between 6-10mg, megalobrama amblycephala adult fish bone scale and organ have all been developed completely, and the Yellow River carp postlarva bone scale is at development
In the process.Postlarva intermuscular bone is very thin compared with adult fish, calcification degree is low, and alizarin red is difficult in conjunction with the calcium on intermuscular bone, intermuscular bone
Not easy coloring；Postlarva muscle easy coloring, during muscle fades, intermuscular bone also easily fades therewith.Therefore in fixed preparation, go
Except body surface scale and pigment, the bond strength of intermuscular bone and alizarin red dye liquor is removed internal organs, and muscle is transparent and observation intermuscular bone etc.
Aspect, adult fish intermuscular bone and the dyeing of postlarva intermuscular bone have larger difference.
Summary of the invention
The object of the present invention is to provide a kind of methods, and simple, easily operated and agents useful for same is inexpensively easy to the Yellow River of purchase
The method of carp postlarva intermuscular bone dyeing, it is transparent that this method is easily obtained body, and intermuscular bone position can be obviously observed with stereoscope
The Yellow River carp postlarva sample, the Yellow River carp postlarva intermuscular bone ossification and other cyprinid fish postlarva intermuscular bones ossification research in have
Significance.
The present invention adopts the following technical scheme that achieve the above object, a kind of method of the Yellow River carp postlarva intermuscular bone dyeing,
It is characterized in that detailed process are as follows:
(1) it draws materials to 22-26 days after membrane small fish, and fixes 24 hours with the PFA of 3wt%-5wt%；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 20-30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution, the time is appropriately extended according to the excessive degree of fish body, until head
Portion is transparent；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, is dyed in 19-31 DEG C, dyeing time 8-12 hours；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 1-2 times, until distilled water is not obvious
Color；
(8) hydrogen peroxide being diluted with 1wt% KOH solution, it is 1%-5% that hydrogen peroxide, which is diluted to volumetric concentration, it is irradiated under strong light, until
Until a large amount of bubbles occurs in fish body surface, fish body surface pigment is sloughed, this process 5-20 minutes；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) it is transparent that the Yellow River carp postlarva is placed to gradient in the glycerine water solution of volume fraction 20%, 50%, it is 0-30 minutes transparent；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol.
Further preferably, the method for the Yellow River carp postlarva intermuscular bone dyeing, it is characterised in that specific steps are as follows:
(1) it draws materials to 24 days after membrane small fish, and fixes 24 hours with 4wt% PFA；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution, the time is appropriately extended according to the excessive degree of fish body, until head
Portion is transparent；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, and 25 DEG C of room temperature dye, dyeing time 10 hours；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 1-2 times, until distilled water is not obvious
Color；
(8) hydrogen peroxide is diluted with 1wt% KOH solution, it is 3% that hydrogen peroxide, which is diluted to volumetric concentration, is irradiated under strong light, until fish
Until the bright a large amount of bubbles of appearance of body surface, fish body surface pigment is taken off, this process 10 minutes；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) it is transparent that the Yellow River carp postlarva is placed to gradient in the glycerine water solution of volume fraction 20%, 50%, it transparent 30 minutes, can root
Extend clearing time in the glycerine water solution of volume fraction 50% according to shade；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol of volume fraction.
The present invention has the following advantages and beneficial effects: that the present invention uses optimal reagent, by different dyeing conditions
Exploration, filter out optimal dyeing condition, it is final to obtain that body is transparent, intermuscular bone dyeing is young for red or purple the Yellow River carp
The high-quality sample of fish can clearly observe intermuscular bone attachment position with body formula mirror, and only postlarva intermuscular bone Senile Mouse does not mention
For convenient, it can also be used to which section is universal to be used as the art work.
Detailed description of the invention
Fig. 1 is the Yellow River carp postlarva intermuscular bone dyeing overall schematic；
Fig. 2 is the Yellow River carp postlarva tail portion intermuscular bone schematic diagram, it may be clearly seen that epineurals and epipleurals；
Fig. 3 is the schematic diagram of the Yellow River carp postlarva tail portion epipleurals；
Fig. 4 is the schematic diagram of the Yellow River carp postlarva head epineurals；
Fig. 5-7 is the schematic diagram that unspent hydrogen peroxide fades；
Fig. 8-10 is 6 hours schematic diagrames of dyeing, and postlarva intermuscular bone is obviously uncolored；
Figure 11-12 is 8 hours schematic diagrames of dyeing, and it is uncolored that postlarva intermuscular bone dyes unintelligible and head；
Figure 13-16 is 19 DEG C, 25 DEG C, 31 DEG C and 37 DEG C dyeing effect figures respectively；
Figure 17-20 is the hydrogen peroxide difference fading time figure of 1wt%, 3wt%, 5wt% with 7wt% respectively；
Figure 21 is glycerol difference clearing time figure.
Specific embodiment
Below in conjunction with the attached drawing in the present invention, the technical solution in specific implementation process of the present invention is carried out clear, complete
Whole, specific description.
Embodiment 1
Intermuscular bone dyeing theory is that chelatropic reaction occurs for the hydroxy-anthraquione structure of alizarin red and calcium ion, generates a kind of colored chemical combination
The calcium tubercle of object, the outer layer of such osteoblast induction has just dyed peony.For a kind of chemical reaction, reactant and bottom are removed
Other than object, it is influenced maximum to be reaction temperature and reaction time.Therefore the present embodiment is respectively to temperature and dyeing time
Influence to the Yellow River carp postlarva intermuscular bone dyeing effect carried out analysis assessment, and selected optimal dyeing temperature and dyeing when
Between.
Pass through the effect picture to 6,8,10 hours different dyeing times and 19 DEG C, 25 DEG C, 31 DEG C, 37 DEG C different dyeing temperatures
It is analyzed, explores the method that can obtain optimum dyeing quality.
6 hours, 8 hours, 10 hours different dyeing times, dyeing effect comparison:
(1) materials are fixed, and are drawn materials to postlarva, and fix 24 hours with 4wt% PFA, wherein PFA is that the paraformaldehyde of 3-5g is molten
In PBS, pH is adjusted to 7.2；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution, according to the fish body excessive proper extension time, until head is saturating
It is bright；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, and 25 DEG C of room temperature dyeing, dyeing time is 6 hours, 8 hours, 10 hours respectively；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 2 times；
(8) hydrogen peroxide is diluted with 1wt% KOH solution, hydrogen peroxide is diluted to volumetric concentration 3%, is irradiated under strong light, until fish body
Until showing bubble occur, fish body surface pigment is sloughed, this process 15 minutes can be appropriately extended according to fish body size；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) the Yellow River carp postlarva is placed to gradient dilution in the glycerine water solution of volume fraction 20%, 50%；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol of volume fraction.
It dyes 6 hours coloration results and refers to Fig. 8-10.
Dye 8 hours coloration results 1-12 referring to Figure 1.
Dye 10 hours coloration results referring to Figure 1-3.
By to different dyeing times it was found that, more preferable (the intermuscular bone coloring and more clearly of the effect of dyeing 10 hours
It is clear).
19 DEG C, 25 DEG C, 31 DEG C, 37 DEG C of different dyeing temperatures, dyeing effect comparison:
(1) materials are fixed, and are drawn materials to postlarva, and fix 24 hours with 4wt% PFA；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body arrives 1wt% KOH solution, impregnates 30 minutes, according to fish body it is excessive can the extension time appropriate, up to head
Portion is transparent；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor be 3:1, dyeing time 10 hours, and be arranged 19 DEG C of different temperature gradients, 25 DEG C, 31 DEG C, 37 DEG C into
Row dyeing；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 2 times；
(8) hydrogen peroxide is diluted with 1wt% KOH solution, hydrogen peroxide is diluted to volumetric concentration 3%, is irradiated under strong light, until fish body
Until a large amount of bubbles occurs in surface, fish body surface pigment is taken off, this process 15 minutes can be appropriately extended according to fish body size；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) the Yellow River carp postlarva is placed to gradient dilution in the glycerine water solution of volume fraction 20%, 50%；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol of volume fraction.
Dyeing effect figure is referring to Figure 13-16.
Head meat and lipid are thicker, and intermuscular bone is coloured compared with tail portion difficulty, pass through the comparison to head intermuscular bone staining conditions
It was found that 25 DEG C of compared with 19 DEG C, 31 DEG C and 37 DEG C dyeing effects are more preferable.
Embodiment 2
Fish body surface pigment generates obstruction to the observation of postlarva intermuscular bone, and hydrogen peroxide can remove fish body surface pigment, convenient for observation
The intermuscular bone of dyeing.The present embodiment explores optimal hydrogen peroxide concentration by fading time.
Optimization to hydrogen peroxide concentration and bleaching time utilizes the dioxygen of the various concentrations such as 1wt%, 3wt%, 5wt%, 7wt%
Water observes fading time and fading effect, selects optimal colour fading concentration:
(1) materials are fixed, and are drawn materials to postlarva, and fix 24 hours with 4wt% PFA；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, is dyed 10 hours；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 2 times；
(8) hydrogen peroxide is diluted with 1wt% KOH solution, it is 1%, 3%, 5% and 7% that hydrogen peroxide, which is diluted to volumetric concentration, under strong light
Irradiation, until fish body shows bubble occur, sloughs fish body surface pigment, observes each concentration bleaching time and decolorizing effect；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) the Yellow River carp postlarva is placed to gradient dilution in the glycerine water solution of volume fraction 20%, 50%；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol of volume fraction.
Coloration result 7-20 referring to Figure 1.
Although hydrogen peroxide of the volumetric concentration 5% compared with 3% and 1% can take off fish body surface pigment in a shorter time,
Influence in view of 7% heavy corrosion fish body surface of volumetric concentration and in view of volumetric concentration 5% to intermuscular bone, so we select temperature
With and colour fading concentration of the relatively good volumetric concentration 3% of effect as hydrogen peroxide.
By with 20% glycerine water solution of volume fraction it is 0 minute transparent, 10 minutes, 30 minutes, 50 minutes it has been observed that thoroughly
Bright 0-30 minutes effect is preferable, but the depth that can be coloured according to fish body extension appropriate is in 50% glycerine water solution of volume fraction
Clearing time, specific figure refers to Figure 21.
Basic principle of the invention, main feature and advantage has been shown and described above, do not depart from spirit of that invention and
Under the premise of range, there are also various changes and modifications, these changes and improvements to both fall within claimed invention by the present invention
Range.
Claims (2)
1. a kind of method of the Yellow River carp postlarva intermuscular bone dyeing, it is characterised in that detailed process are as follows:
(1) draw materials to 22-26 days after membrane small fish, and fix 24 hours with the PFA of 3wt%-5wt%, wherein PFA be by
The paraformaldehyde of 3-5g is dissolved in PBS, and pH is adjusted to 7.2；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 20-30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution, the time is appropriately extended according to the excessive degree of fish body, until head
Portion is transparent；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, is dyed in 19-31 DEG C, dyeing time 8-12 hours；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 1-2 times, until distilled water is not obvious
Color；
(8) hydrogen peroxide being diluted with 1wt% KOH solution, it is 1%-5% that hydrogen peroxide, which is diluted to volumetric concentration, it is irradiated under strong light, until
Until a large amount of bubbles occurs in fish body surface, fish body surface pigment is sloughed, this process 5-20 minutes；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) it is transparent that the Yellow River carp postlarva is placed to gradient in the glycerine water solution of volume fraction 20%, 50%, it is 0-30 minutes transparent；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol.
2. the method for the Yellow River carp postlarva intermuscular bone dyeing according to claim 1, it is characterised in that specific steps are as follows:
(1) it draws materials to 24 days after membrane small fish, and fixes 24 hours with 4wt% PFA；
(2) fish body first is rinsed with distilled water before dyeing, 10 minutes every time, rinsing three times, removed fixer；
(3) fish body is retransferred to rinse 30 minutes into TBST solution；
(4) it is then rinsed 10 minutes with distilled water, removes remaining TBST；
(5) transfer fish body is impregnated 30 minutes to 1wt% KOH solution, the time is appropriately extended according to the excessive degree of fish body, until head
Portion is transparent；
(6) transfer fish body adds alizarin red dye liquor and is dyed into new 1wt% KOH solution, KOH solution and alizarin red
The volume ratio of dye liquor is 3:1, and 25 DEG C of room temperature dye, dyeing time 10 hours；
(7) dyeing liquor is outwelled, distilled water is added, washes away extra dyeing liquor, repetition is washed 1-2 times, until distilled water is not obvious
Color；
(8) hydrogen peroxide is diluted with 1wt% KOH solution, it is 3% that hydrogen peroxide, which is diluted to volumetric concentration, is irradiated under strong light, until fish
Until the bright a large amount of bubbles of appearance of body surface, fish body surface pigment is sloughed, this process 10 minutes；
(9) solution is outwelled, 1wt% KOH solution is added, washes away remaining hydrogen peroxide；
(10) it is transparent that the Yellow River carp postlarva is placed to gradient in the glycerine water solution of volume fraction 20%, 50%, it transparent 30 minutes, can root
Extend clearing time in the glycerine water solution of volume fraction 50% according to shade；
(11) with body formula sem observation coloration result and retention of taking pictures；
(12) the Yellow River carp postlarva is saved in 100% glycerol of volume fraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910891975.0A CN110495445A (en) | 2019-09-20 | 2019-09-20 | A kind of method of the Yellow River carp postlarva intermuscular bone dyeing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910891975.0A CN110495445A (en) | 2019-09-20 | 2019-09-20 | A kind of method of the Yellow River carp postlarva intermuscular bone dyeing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110495445A true CN110495445A (en) | 2019-11-26 |
Family
ID=68592294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910891975.0A Pending CN110495445A (en) | 2019-09-20 | 2019-09-20 | A kind of method of the Yellow River carp postlarva intermuscular bone dyeing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110495445A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112729982A (en) * | 2020-12-21 | 2021-04-30 | 上海海洋大学 | Preparation method of material for identifying age of shark |
| CN113115728A (en) * | 2021-03-26 | 2021-07-16 | 广西壮族自治区水产科学研究院 | Transparent carp breeding method |
| CN113881784A (en) * | 2021-11-11 | 2022-01-04 | 中国水产科学研究院 | Method for rapidly identifying yellow river carp carrying red mutant allele |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050202524A1 (en) * | 2004-03-15 | 2005-09-15 | Brusilovskiy Arkadiy I. | Tissue staining protocol and composition |
| CN102519774A (en) * | 2011-11-16 | 2012-06-27 | 中国水产科学研究院东海水产研究所 | Dyeing method for peripheral nerves of fish larvae and juveniles |
| CN104872110A (en) * | 2015-05-28 | 2015-09-02 | 暨南大学 | Crystal skeleton specimen manufacturing method |
| CN105532642A (en) * | 2015-12-25 | 2016-05-04 | 华中农业大学 | Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method |
| CN107258764A (en) * | 2017-06-16 | 2017-10-20 | 大连海洋大学 | Cartilaginous fish skeleton specimen preparation method |
| CN109845721A (en) * | 2019-04-01 | 2019-06-07 | 中国海洋大学 | A kind of production method of siphonopods transparent specimen |
| CN110089516A (en) * | 2019-05-28 | 2019-08-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of production method of novel larva and juvenile sample |
-
2019
- 2019-09-20 CN CN201910891975.0A patent/CN110495445A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050202524A1 (en) * | 2004-03-15 | 2005-09-15 | Brusilovskiy Arkadiy I. | Tissue staining protocol and composition |
| CN102519774A (en) * | 2011-11-16 | 2012-06-27 | 中国水产科学研究院东海水产研究所 | Dyeing method for peripheral nerves of fish larvae and juveniles |
| CN104872110A (en) * | 2015-05-28 | 2015-09-02 | 暨南大学 | Crystal skeleton specimen manufacturing method |
| CN105532642A (en) * | 2015-12-25 | 2016-05-04 | 华中农业大学 | Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method |
| CN107258764A (en) * | 2017-06-16 | 2017-10-20 | 大连海洋大学 | Cartilaginous fish skeleton specimen preparation method |
| CN109845721A (en) * | 2019-04-01 | 2019-06-07 | 中国海洋大学 | A kind of production method of siphonopods transparent specimen |
| CN110089516A (en) * | 2019-05-28 | 2019-08-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of production method of novel larva and juvenile sample |
Non-Patent Citations (7)
| Title |
|---|
| 吕耀平等: "唇䱻肌间小骨的骨化过程", 《上海海洋大学学报》 * |
| 吕耀平等: "瓯江彩鲤肌间小骨的骨化模式", 《上海海洋大学学报》 * |
| 柯中和等: "鲢肌间小骨发育的形态学观察", 《动物学杂志》 * |
| 王静等: "合方鲫肌间骨骨化过程及形态学观察", 《湖南师范大学自然科学学报》 * |
| 秉志: "幼鲤大侧肌隔骨针的观察", 《动物学报》 * |
| 聂春红等: "不同鱼类肌间骨的骨化模式研究", 《水生生物学报》 * |
| 陈琳等: "黄河鲤肌间骨发育的形态学观察", 《上海海洋大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112729982A (en) * | 2020-12-21 | 2021-04-30 | 上海海洋大学 | Preparation method of material for identifying age of shark |
| CN113115728A (en) * | 2021-03-26 | 2021-07-16 | 广西壮族自治区水产科学研究院 | Transparent carp breeding method |
| CN113881784A (en) * | 2021-11-11 | 2022-01-04 | 中国水产科学研究院 | Method for rapidly identifying yellow river carp carrying red mutant allele |
| CN113881784B (en) * | 2021-11-11 | 2023-06-20 | 中国水产科学研究院 | Method for rapidly identifying yellow river carp carrying red mutant allele |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110495445A (en) | A kind of method of the Yellow River carp postlarva intermuscular bone dyeing | |
| CN106644656B (en) | One step decoration method of Hematoxylin-eosin | |
| DE60006685T2 (en) | RED EMITTERING [8,9] BENZOPHENOXAZINE NUCLEIC ACID DYES AND METHOD FOR USE THEREOF | |
| Shimizu et al. | A novel fluorescent silica tracer for biological silicification studies | |
| CN105651580B (en) | A kind of dyed blended liquid in haematoxylin Yihong | |
| Shukla et al. | Natural dyes from Himalayan lichens | |
| CN108375599A (en) | A kind of transmission electron microscope sample flaking method of microminiature Compound Eye of Insects | |
| CN103525119A (en) | Cartilage staining solution, bone staining method and method for preparing embryonic bone specimen by using bone staining method | |
| CN103808550B (en) | A kind of Myxosporean dyeing method of seal being easy to morphological observation | |
| CN104977194B (en) | A method of addition graphene accelerates sample process | |
| CN105131647B (en) | A kind of dye composite, application and its application method for biological HE dyeing | |
| CN104872110A (en) | Crystal skeleton specimen manufacturing method | |
| CN105842037B (en) | Colouring method that is a kind of while showing mast cell and acidophic cell | |
| CN106323723B (en) | Double indigo plant decoration methods | |
| CN110089516A (en) | A kind of production method of novel larva and juvenile sample | |
| CN108106909B (en) | Biological tissue light transparentizing agent, light transparentizing method and application thereof | |
| Itodo et al. | Phytochemical properties and staining ability of red onion (Allium cepa) extract on histological sections | |
| CN105241727B (en) | Periodic acid-Xue Fu (PAS) dyeing liquor (chemical dyeing method) | |
| Rani et al. | A potential benefit of brown seaweed (Stoechospermum marginatum) using for sustainable fabric dyeing. | |
| CN1961816A (en) | Method for making fetus transparent specimen | |
| Mustafa et al. | Kermes dye extract from Coccus ilicis insect as an alternative counter stain instead of eosin in various tissue constituents: An experimental study | |
| CN114279795A (en) | Rapid detection system, detection method and application of tissue sample | |
| CN110196181A (en) | The application of silver hexamine staining kit and its colouring method, protected protein agent in silver hexamine staining field | |
| CN108318311B (en) | Hard bone specific calcium fluorescent staining method and application thereof | |
| CN109490268A (en) | A kind of fluorescence microscopy method of efficient, high-definition observation of plant pollen tube growing state in gynoecium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |