CN109946140A - Fungi dyeing liquor and fungi colouring method - Google Patents
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Abstract
The present invention relates to fungi dyeing liquors and fungi colouring method.The dyeing liquor is divided into A liquid and B liquid；A liquid is potassium hydroxide aqueous solution, and the concentration of potassium hydroxide is 9~15wt% in A liquid；The gentiobiose that A liquid is also 0.1~0.3wt% containing concentration；B liquid is the methylene blue solution of phenol, and the concentration of methylenum careuleum is 0.1wt% in B liquid, and the concentration of phenol is 2~3vol%, the L lysine HCL that B liquid is also 0.1~0.3wt% containing concentration in B liquid.Fungi dyeing liquor of the invention has the advantages that dyeing liquor dosage is few when in use, and dyeing effect is good, and fine oil droplets and fungi very can be efficiently distinguished in the detection of fungi, improves efficiency of the hospital in related check work, is suitble to promote.Corresponding, the present invention also provides the methods for using fungi dyeing liquor of the invention to dye fungi, and application of the fungi dyeing liquor of the present invention in the direct Microscopical Method For Detection of fungi identifies.
Description
Technical field
The present invention relates to medical science, in particular to fungi dyeing liquor and and using the fungi dyeing liquor to fungi into
The method of row dyeing.
Background technique
In the laboratory of hospital, clinical laboratory staff is frequently necessary to leukorrhea, ear canal secretion, first bits, scurf etc.
Substance is tested.When examining, need to distinguish background material and fungi, to detect fungi.But some samples, containing few
The fat drips of amount are easy to appear very tiny oil droplet because fat drips content is few, and these very tiny oil droplets, should not with it is true
Bacterium spore distinguishes.And existing staining technique is when handling outpatient service sample, nothing cumbersome to fungi and fungal spore dying operation
Method provides inspection result in a short time.Therefore it provides a kind of efficient dyeing liquor becomes urgent with a kind of quick detection method
Not as good as to.
Summary of the invention
In order to overcome the above-mentioned deficiency in the presence of the prior art, the present invention provides fungi dyeing liquor, it has and makes
The few advantage of used time dyeing liquor dosage, and dyeing effect is good, very can efficiently be distinguished in the detection of fungi fine oil droplets with
Fungi improves efficiency of the hospital in related check work, is suitble to promote.Corresponding, the present invention also provides use this hair
The method that bright fungi dyeing liquor dyes fungi, and fungi dyeing liquor of the present invention is in the direct Microscopical Method For Detection of fungi identifies
Application；Carrying out dyeing to fungi using method of the invention can increase the sensitivity and shortening detection time of dyeing, be suitble to
It applies and promotes in the detection of fungi dyeing.
For dyeing liquor, the technical scheme is that fungi dyeing liquor, the dyeing liquor is divided into A liquid and B liquid；The A
Liquid is potassium hydroxide aqueous solution, and the concentration of potassium hydroxide is 9~15wt% in the A liquid；Also it is containing concentration in the A liquid
The gentiobiose of 0.1~0.3wt%；The B liquid is the methylene blue solution of phenol, in the B liquid concentration of methylenum careuleum be 0.1~
0.2wt%, the concentration of phenol is 2~3vol%, the L- for being also 0.1~0.3wt% containing concentration in the B liquid in the B liquid
Lysine hydrochloride；
The method for preparing the fungi dyeing liquor, is divided into following steps:
1), prepared by A liquid:
1. weighs 18~30g chemistry pure cerium hydroxide potassium in the balance and is placed in a beaker, it is slowly added to deionized water to 200ml,
It is gently mixed, is allowed to dissolve, the potassium hydroxide solution of 9~15wt% concentration is made；
2. after prepares potassium hydroxide solution, adding 0.2~0.6g of gentiobiose；After solution-stabilized, it is transferred to modeling
Expect in reagent bottle, A liquid is made；
2), prepared by B liquid:
1. takes 4~6ml phenol, add distilled water to 100ml, firmly shaking is placed in 37 DEG C of incubators 1~2 day, to complete
To room temperature preservation in brown bottle after dissolution, the phenol solution of 4~6vol% concentration is made；
2. weighs 1~2g of methylenum careuleum of drying, adds distilled water to 1000ml, be then heated to 35~40 DEG C, stirring to Asia
First indigo plant is completely dissolved, and is cooled to room temperature；
3. the phenol solution that will be prepared is mixed with aqueous solution of methylene blue with volume ratio 1:1, be made phenol concentration be 2~
The methylene blue solution of 3vol%；
4. phenol concentration obtained be 2~3vol% methylene blue solution in add L lysine HCL 0.2~
0.6g；It after solution-stabilized, is transferred in plastics reagent bottle, B liquid is made.
Compared with prior art, fungi dyeing liquor of the invention has following significant progress:
1) fungi dyeing liquor of the invention is divided into A liquid and B liquid, this has the advantages of preparation is simple and convenient to operate, and can contract
The efficiency of the short time for preparing dyeing liquor and raising fungi dyeing liquor in fungi dyeing course；
2) specific component formula so that fungi dyeing liquor of the invention has the advantages that dosage is few when in use, and is tried
While the cost of agent is low, also have the characteristics that dyeing effect is good, the tiny oil droplet and allergenic after the dyeing of this dyeing liquor
The identification of son is high, this makes it very can efficiently distinguish fine oil droplets and fungi in the detection of fungi.
3) gentiobiose can promote potassium hydroxide to the cauterization effect of the sample of required dyeing, accelerate test sample institute
The time of alkaline denatured protein is needed to form, L lysine HCL can make endonuclear chromatin easy and dyeing liquor
In conjunction with improving dyeing effect.
One of preferred embodiment: in fungi dyeing liquor above-mentioned, in the A liquid, the concentration of potassium hydroxide is 10wt%, rough gentian
The concentration of disaccharides is 0.2wt%；In the B liquid, the concentration of the concentration 0.1wt% of methylenum careuleum, phenol are 2.5vol%, and L- relies ammonia
The concentration of acid hydrochloride is 0.2wt%.At this point, gentiobiose can be more efficiently promoting potassium hydroxide to the sample of required dyeing
Cauterization effect, the time of alkaline denatured protein is formed required for acceleration detection sample, L lysine HCL can make carefully
Chromatin in karyon is more easier in conjunction with dyeing liquor, thus, improve dyeing effect.
The two of preferred embodiment: in fungi dyeing liquor above-mentioned, in the B liquid, the concentration of methylenum careuleum is 0.1wt%, phenol
Concentration be 2.5vol%, the concentration of L lysine HCL is 0.2wt%.At this point, L lysine HCL can make cell
Chromatin in core is more easier in conjunction with dyeing liquor, improves dyeing effect.
The three of preferred embodiment: in fungi dyeing liquor above-mentioned, in the A liquid, the concentration of potassium hydroxide is 15wt%, rough gentian
The concentration of disaccharides is 0.3wt%；In the B liquid, the concentration of the concentration 0.1wt% of methylenum careuleum, phenol are 2.5vol%, and L- relies ammonia
The concentration of acid hydrochloride is 0.2wt%.At this point, the cauterization effect of A liquid is strong, it is suitable for some first are considered to be worth doing or the needs such as scurf are more etc.
To the sample of time, shorten the time that alkaline denatured protein is formed required for test sample.
For colouring method, technical solution of the present invention first is that:
Using the fungi dyeing liquor of one of aforementioned preferred embodiment of the invention in vaginal fluid or ear canal secretion
The method that is dyed of fungi, comprising the following steps:
Step 1, take vaginal fluid or ear canal secretion that 0.2~0.3ml physiological saline is originally added to be made smear, under microscope
Observe cleannes；
Step 2, on piece is being applied to the sample dropwise addition drop of A liquid 1~2, mixing, mixing time is 5~10S；
Step 3, then B liquid 1~2 is added dropwise to sample to drip, mixes, mixing time is 10~15S, under the microscope.This detection side
Method has the advantages of easy implementation easy to operate, can extremely be rapidly performed by the dyeing to sample, so that tiny oil droplet and fungi
Spore is clearly distinguished and is come, and is accelerated screening and detection speed in clinical sample, is suitble in the detection that fungi is dyed
Using and promote.
For colouring method, technical solution of the present invention second is that:
The method that fungi in fungal cultures is dyed using two B liquid of aforementioned preferred embodiment of the invention,
The following steps are included:
Step 1: on glass slide, B liquid 1~2 is added dropwise and drips；
Step 2: taking part filamentous fungi with adhesive tape is viscous；
Step 3: viscous acquirement fungi being placed directly against on the B liquid of glass slide, is observed under the microscope after 1min.Detect fungi
The operation of pure culture is more simple, guarantees to also reduce usage amount and the operating time of dyeing liquor while dyeing effect.
For colouring method, technical solution of the present invention third is that:
Fungi in first bits or scurf sample is carried out using three fungi dyeing liquor of aforementioned preferred embodiment of the invention
The method of dyeing, comprising the following steps:
Step 1: taking first to consider to be worth doing with pincet or scurf is a little or disease sends out one, be placed in glass slide center, A liquid 1~2 is added dropwise
Drop, stays a minute；
Step 2: glass slide being placed on micro- heating above flame, dissolves tissue or cutin；
Step 3: after cooling, 1~2 drop B liquid is added dropwise, covered simultaneously compresses, the mirror after histolysis disperses and is transparent
Lower observation.The operation of detection first bits or scurf needs to dissociate sample in order to preferably carry out subsequent dyeing and sight
It examines.Further, in the step 1, the amount of A liquid is added dropwise as 2 drops, in the step 3, the amount that B liquid is added dropwise is 1 drop.Increase A liquid
Usage amount for improving dyeing efficiency, shorten the time for first bits or scurf class sample detection.
Meanwhile the answering in the direct Microscopical Method For Detection of fungi identifies the present invention also provides aforementioned fungi dyeing liquor of the invention
With.The method is suitble to be extended to the detection in direct Microscopical Method For Detection to fungi；Compared with other colouring methods, the operation of two steps is only needed
Step has the advantages that dyeing time is short, is suitble to the needs such as Emergency call quickly to go out the department according to inspection result, in for detecting
The cost for reducing many man power and materials has been more convenient to examine by development bed.
Detailed description of the invention
Fig. 1 is undyed leukorrhea sample effect picture；
Fig. 2 is the leukorrhea sample effect picture being added dropwise after A liquid；
Fig. 3 is the leukorrhea sample effect picture after dyeing；
Fig. 4 is undyed fungal cultures effect picture under high power lens；
Fig. 5 is the fungal cultures effect picture after dyeing under low power lens；
Fig. 6 is the fungal cultures effect picture after dyeing under high power lens.
Specific embodiment
Below by specific embodiment, and in conjunction with attached drawing, technical solution of the present invention is further elaborated with, but simultaneously
Not as the foundation limited the present invention.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art.
Fungi dyeing liquor of the invention is divided into A liquid and B liquid；The A liquid is potassium hydroxide aqueous solution, hydrogen-oxygen in the A liquid
The concentration for changing potassium is 10wt%；The gentiobiose for being also 0.2wt% containing concentration in the A liquid；The B liquid is the methylene of phenol
Blue solution, the concentration of methylenum careuleum is 0.1wt% in the B liquid, and the concentration of phenol is 2.5vol% in the B liquid, in the B liquid
The L lysine HCL for being also 0.2wt% containing concentration；The method for preparing the fungi dyeing liquor, is divided into following steps:
1), prepared by A liquid:
1. weighs 20g chemistry pure cerium hydroxide potassium in the balance and is placed in a beaker, it is slowly added to deionized water to 200ml, gently
Stirring, is allowed to dissolve, and the potassium hydroxide solution of 10wt% concentration is made；
2. after prepares potassium hydroxide solution, adding gentiobiose 0.4g；After solution-stabilized, it is transferred to plastics examination
In agent bottle, A liquid is made；
2), prepared by B liquid:
1. takes 5ml phenol, add distilled water to 100ml, firmly shaking is placed in 37 DEG C of incubators 1~2 day, to completely molten
The phenol solution of 5vol% concentration is made in room temperature preservation in Xie Houzhi brown bottle；
2. weighs the methylenum careuleum 1g of drying, adds distilled water to 1000ml, be heated to 35~40 DEG C, stir complete to methylenum careuleum
Fully dissolved is cooled to room temperature；
3. the phenol solution that will be prepared is mixed with aqueous solution of methylene blue with volume ratio 1:1, obtained phenol concentration is
The methylene blue solution of 2.5vol%；
4. adds L lysine HCL 0.3g in the methylene blue solution that phenol concentration obtained is 2.5vol%；
It after solution-stabilized, is transferred in plastics reagent bottle, B liquid is made.
Application examples 1:
The dyeing liquor that the application example uses is divided into A liquid and B liquid；The A liquid is potassium hydroxide aqueous solution, hydrogen in the A liquid
The concentration of potassium oxide is 10wt%；The gentiobiose for being also 0.2wt% containing concentration in the A liquid；The B liquid is the Asia of phenol
First indigo plant solution, the concentration of methylenum careuleum is 0.1wt% in the B liquid, and the concentration of phenol is 2.5vol%, the B liquid in the B liquid
In also containing concentration be 0.2wt% L lysine HCL.
The detection sample of the application example is vaginal fluid or ear canal secretion, the specific steps are as follows:
Step 1, sampling is originally plus smear, microscopically observation cleannes are made in 0.2ml physiological saline；
Step 2, on piece is being applied to the sample dropwise addition drop of A liquid 1,0.1ml is mixed, time 7S；
Step 3, then B liquid 1 is added dropwise to sample to drip, mixes, mixing time 12S, under the microscope.It is white referring to Fig. 1 to Fig. 3
Band sample: under the microscope, karyocyte is all destroyed, and fungi and fungal spore are dyed to navy blue, and fat drips are not colored.
Application examples 2:
The application example detects fungal cultures sample using B liquid of the invention；The B liquid is the methylenum careuleum of phenol
Solution, the concentration of methylenum careuleum is 0.1wt% in the B liquid, and the concentration of phenol is 2.5vol% in the B liquid, in the B liquid also
The L lysine HCL for being 0.2wt% containing concentration.
Fungal cultures sample is detected according to the following steps:
Step 1: on glass slide, B liquid 1 is added dropwise and drips；
Step 2: taking part filamentous fungi with adhesive tape is viscous；
Step 3: viscous acquirement fungi being placed directly against on the B liquid of glass slide, is observed under the microscope after 1min.Referring to fig. 4
To Fig. 6, fungi pure culture, the fungi after dyeing can be by clear discrimination.
Application examples 3:
The fungi dyeing liquor of the application example is divided into A liquid and B liquid；The A liquid is potassium hydroxide aqueous solution, hydrogen in the A liquid
The concentration of potassium oxide is 15wt%；The gentiobiose for being also 0.3wt% containing concentration in the A liquid；The B liquid is the Asia of phenol
First indigo plant solution, the concentration of methylenum careuleum is 0.1wt% in the B liquid, and the concentration of phenol is 2.5vol%, the B liquid in the B liquid
In also containing concentration be 0.2wt% L lysine HCL.
First bits or scurf sample are detected according to the following steps:
Step 1: taking first to consider to be worth doing with pincet or scurf is a little or disease sends out one, be placed in glass slide center, A liquid 1 is added dropwise and drips, slightly
To a moment；
Step 2: glass slide being placed on micro- heating above flame, dissolves tissue or cutin；
Step 3: after cooling, 1 drop B liquid is added dropwise, covered simultaneously compresses, and sees under mirror after histolysis disperses and is transparent
It examines.
Points for attention:
1, potassium hydroxide solution low dose of must dispense when in use easily with the carbon dioxide reaction in air, avoid frequency
Numerous folding reagent bottle and cause potassium hydroxide solution effective concentration reduction；
If 2, vial is selected to load the potassium hydroxide solution prepared, ground glass stopper, SiO cannot be used2+2KOH
=K2SiO3+H2O after being long placed in, is easy to make vial and ground glass stopper stick to each other, therefore should select Plastic bottle plug.
3, potassium hydroxide is woven with cauterization effect to group, can dissolve protein, forms alkaline denatured protein, when in use,
Rubber gloves should be put on, properly protect measure.
4, phenol exposure is oxidized by oxygen in air as quinone and pinkiness, low dose of must be dispensed, be kept away when in use
Exempt from frequently to open and close reagent bottle and lead to the reduction of B liquid effective concentration；
5, it is slightly soluble in water under phenol room temperature, when temperature is higher than 65 DEG C, can be dissolved each other with water with arbitrary proportion, in winter room temperature
It is lower, it easily crystallizes in reagent bottle, B liquid should be set in 37 DEG C of incubators 1 hour before use, to improve the concentration of phenol in B liquid,
To enhance colouring power；
6, phenol has strong corrosiveness to skin, mucous membrane, can inhibit nervous centralis or damage Liver and kidney function.It should keep away
Exempt to use in closed environment, pays attention to ventilation.
7, small lot dispenses, and can preferably save reagent, effective component is avoided to reduce.
The above-mentioned generality to invention involved in the application is described and be should not be construed as to the description of its specific embodiment
It is the limitation constituted to the inventive technique scheme.Those skilled in the art according to disclosure herein, can without prejudice to
Under the premise of related invention constituent element, the public technology feature in above-mentioned general description or/and embodiment is carried out
Increase, reduce or combine, forms the other technical solutions belonged within the application protection scope.
Claims (9)
1. fungi dyeing liquor, it is characterised in that: the dyeing liquor is divided into A liquid and B liquid；The A liquid is potassium hydroxide aqueous solution, institute
The concentration for stating potassium hydroxide in A liquid is 9~15wt%；The gentiobiose for being also 0.1~0.3wt% containing concentration in the A liquid；
The B liquid is the methylene blue solution of phenol, and the concentration of methylenum careuleum is 0.1~0.2wt% in the B liquid, phenol in the B liquid
Concentration is 2~3vol%, the L lysine HCL for being also 0.1~0.3wt% containing concentration in the B liquid；It prepares described true
The method of bacterium dyeing liquor, is divided into following steps:
1), prepared by A liquid:
1. weighs 18~30g chemistry pure cerium hydroxide potassium in the balance and is placed in a beaker, it is slowly added to deionized water to 200ml, gently
Stirring, is allowed to dissolve, and the potassium hydroxide solution of 9~15wt% concentration is made；
2. after prepares potassium hydroxide solution, adding 0.2~0.6g of gentiobiose；After solution-stabilized, it is transferred to plastics examination
In agent bottle, A liquid is made；
2), prepared by B liquid:
1. takes 4~6ml phenol, add distilled water to 100ml, firmly shaking is placed in 37 DEG C of incubators 1~2 day, wait be completely dissolved
Afterwards to room temperature preservation in brown bottle, the phenol solution of 4~6vol% concentration is made；
2. weighs 1~2g of methylenum careuleum of drying, adds distilled water to 1000ml, be then heated to 35~40 DEG C, stirring to methylenum careuleum
It is completely dissolved, is cooled to room temperature；
3. the phenol solution that will be prepared is mixed with aqueous solution of methylene blue with volume ratio 1:1, be made phenol concentration be 2~
The methylene blue solution of 3vol%；
4. phenol concentration obtained be 2~3vol% methylene blue solution in add L lysine HCL 0.2~
0.6g；It after solution-stabilized, is transferred in plastics reagent bottle, B liquid is made.
2. fungi dyeing liquor according to claim 1, it is characterised in that: in the A liquid, the concentration of potassium hydroxide is
10wt%, the concentration of gentiobiose are 0.2wt%；In the B liquid, the concentration of methylenum careuleum is 0.1wt%, and the concentration of phenol is
2.5vol%, the concentration of L lysine HCL are 0.2wt%.
3. fungi dyeing liquor according to claim 1, it is characterised in that: in the B liquid, the concentration of methylenum careuleum is
0.1wt%, the concentration of phenol are 2.5vol%, and the concentration of L lysine HCL is 0.2wt%.
4. fungi dyeing liquor according to claim 1, it is characterised in that: in the A liquid, the concentration of potassium hydroxide is
15wt%, the concentration of gentiobiose are 0.3wt%；In the B liquid, the concentration of methylenum careuleum is 0.1wt%, and the concentration of phenol is
2.5vol%, the concentration of L lysine HCL are 0.2wt%.
5. being dyed using fungi dyeing liquor as claimed in claim 2 to the fungi in vaginal fluid or ear canal secretion
Method, which is characterized in that detection sample is vaginal fluid or ear canal secretion, comprising the following steps:
Step 1, sampling is originally plus smear, microscopically observation cleannes are made in 0.2~0.3ml physiological saline；
Step 2, on piece is being applied to the sample dropwise addition drop of A liquid 1~2, mixing, mixing time is 5~10S；
Step 3, then B liquid 1~2 is added dropwise to sample to drip, mixes, mixing time is 10~15S, under the microscope.
6. the method dyed using B liquid as claimed in claim 3 to the fungi in fungal cultures, which is characterized in that inspection
Test sample sheet is fungal cultures, comprising the following steps:
Step 1: on glass slide, B liquid 1~2 is added dropwise and drips；
Step 2: taking part filamentous fungi with adhesive tape is viscous；
Step 3: viscous acquirement fungi being placed directly against on the B liquid of glass slide, is observed under the microscope after 1min.
7. the method dyed using fungi dyeing liquor as claimed in claim 4 to the fungi in first bits or scurf, feature
It is, detection sample is first bits or scurf, comprising the following steps:
Step 1: taking first to consider to be worth doing with pincet or scurf is a little or disease sends out one, be placed in glass slide center, A liquid 1~2 is added dropwise and drips, slightly
To a moment；
Step 2: glass slide being placed on micro- heating above flame, dissolves tissue or cutin；
Step 3: after cooling, 1~2 drop B liquid is added dropwise, covered simultaneously compresses, and sees under mirror after histolysis disperses and is transparent
It examines.
8. the method that the fungi according to claim 7 in first bits or scurf is dyed, it is characterised in that: the step
In rapid 1, the amount of A liquid is added dropwise as 2 drops, in the step 3, the amount that B liquid is added dropwise is 1 drop.
9. application of the fungi dyeing liquor described in claim 1 in the direct Microscopical Method For Detection of fungi identifies.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110455604A (en) * | 2019-08-28 | 2019-11-15 | 昆氏(深圳)生物科技有限公司 | Epidermis fungi samples colouring method |
| CN110806408A (en) * | 2019-11-14 | 2020-02-18 | 珠海伊斯佳科技股份有限公司 | Method for detecting malassezia scalp |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6503702B1 (en) * | 1993-12-10 | 2003-01-07 | Syngenta Investment Corporation | Rapid immunoassay for detection of antibodies or antigens incorporating simultaneous sample extraction and immunogenic reaction |
| CN105067412A (en) * | 2015-08-10 | 2015-11-18 | 长春瑞克医疗科技有限公司 | Vaginal secretion staining fluid, and preparation method and staining method thereof |
| CN106198470A (en) * | 2016-06-30 | 2016-12-07 | 江苏莱芙时代生物科技有限公司 | A kind of fungal detection fluorescence staining liquid and application |
| CN107286207A (en) * | 2017-08-10 | 2017-10-24 | 济南山目生物医药科技有限公司 | A kind of synthetic method of gentiobiose |
| WO2017208463A1 (en) * | 2016-06-03 | 2017-12-07 | 花王株式会社 | Hair cosmetic |
| CN108168983A (en) * | 2018-03-05 | 2018-06-15 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent reagent of fungi specific stain |
| CN108344613A (en) * | 2018-02-05 | 2018-07-31 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent dyeing reagent marking leukorrhea and cervical exfoliated cell pathogen |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
-
2019
- 2019-04-13 CN CN201910296267.2A patent/CN109946140B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6503702B1 (en) * | 1993-12-10 | 2003-01-07 | Syngenta Investment Corporation | Rapid immunoassay for detection of antibodies or antigens incorporating simultaneous sample extraction and immunogenic reaction |
| CN105067412A (en) * | 2015-08-10 | 2015-11-18 | 长春瑞克医疗科技有限公司 | Vaginal secretion staining fluid, and preparation method and staining method thereof |
| WO2017208463A1 (en) * | 2016-06-03 | 2017-12-07 | 花王株式会社 | Hair cosmetic |
| CN106198470A (en) * | 2016-06-30 | 2016-12-07 | 江苏莱芙时代生物科技有限公司 | A kind of fungal detection fluorescence staining liquid and application |
| CN107286207A (en) * | 2017-08-10 | 2017-10-24 | 济南山目生物医药科技有限公司 | A kind of synthetic method of gentiobiose |
| CN108344613A (en) * | 2018-02-05 | 2018-07-31 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent dyeing reagent marking leukorrhea and cervical exfoliated cell pathogen |
| CN108168983A (en) * | 2018-03-05 | 2018-06-15 | 江苏诺鬲生物科技有限公司 | A kind of fluorescent reagent of fungi specific stain |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
Non-Patent Citations (2)
| Title |
|---|
| DALIA MOEMEN ET AL: ""Fungal keratitis: Rapid diagnosis using methylene blue stain"", 《EGYPTIAN JOURNAL OF BASIC AND APPLIED SCIENCES》 * |
| 史文艳，孙震: ""ε-聚赖氨酸盐酸盐与Nisin对蜡状芽孢杆菌的协同作用及机理"", 《食品与机械》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110455604A (en) * | 2019-08-28 | 2019-11-15 | 昆氏(深圳)生物科技有限公司 | Epidermis fungi samples colouring method |
| CN110455604B (en) * | 2019-08-28 | 2021-02-26 | 昆氏(深圳)生物科技有限公司 | Epidermal fungus sampling and dyeing method |
| WO2021036368A1 (en) * | 2019-08-28 | 2021-03-04 | 昆氏(深圳)生物科技有限公司 | Epidermis fungus sampling and staining method |
| CN110806408A (en) * | 2019-11-14 | 2020-02-18 | 珠海伊斯佳科技股份有限公司 | Method for detecting malassezia scalp |
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