CN108342346A - One plant of Meng Shi pseudomonad and its application - Google Patents

One plant of Meng Shi pseudomonad and its application Download PDF

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CN108342346A
CN108342346A CN201810455504.0A CN201810455504A CN108342346A CN 108342346 A CN108342346 A CN 108342346A CN 201810455504 A CN201810455504 A CN 201810455504A CN 108342346 A CN108342346 A CN 108342346A
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filler
parathion
meng shi
concentration
trichloroacetaldehyde
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CN108342346B (en
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李琳
王彦杰
杨凯雄
刘俊新
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Research Center for Eco Environmental Sciences of CAS
University of Chinese Academy of Sciences
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University of Chinese Academy of Sciences
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/48—Sulfur compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/68—Halogens or halogen compounds
    • B01D53/70—Organic halogen compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2251/00—Reactants
    • B01D2251/95—Specific microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/20—Halogens or halogen compounds
    • B01D2257/206—Organic halogen compounds
    • B01D2257/2064—Chlorine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/30—Sulfur compounds
    • B01D2257/306—Organic sulfur compounds, e.g. mercaptans
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention belongs to field of environmental improvement, and in particular to one plant of Meng Shi pseudomonad and its application.The Meng Shi pseudomonads are specially Meng Shi pseudomonads (Pseudomonas monteilii) LW 1, deposit number is CGMCC No.13748, the bacterial strain is used to handle the organic sulphur waste gas that contains of agricultural chemicals waste water reservoir generation, and gas flow is 100 5000m3/ h, 1.0 5.0min of residence time, 35 55 DEG C of temperature, malathion, parathion, parathion-methyl total concentration be 20 200mg/m3, or trichloroacetaldehyde a concentration of 5 100mg/m3, malathion, parathion, parathion-methyl degradation efficiency can reach 90% or more, and trichloroacetaldehyde degradation efficiency can reach 75% or more.

Description

One plant of Meng Shi pseudomonad and its application
Technical field:
The invention belongs to field of environmental improvement, and in particular to one plant of Meng Shi pseudomonads (Pseudomonas Monteilii) LW-1 and its application in purifying volatile organic sulfur/organochlorine exhaust gas.
Background technology：
Waste water in pesticide producing contains a large amount of organosulfur pesticide and organo-chlorine pesticide intermediate, complicated component, concentration High, strong toxicity.The product or in which mesosome of part organosulfur pesticide and organo-chlorine pesticide such as parathion, malathion, methyl pair Sulphur phosphorus, trichloroacetaldehyde, phosphorus trichloride etc., have a volatility, and most of organic sulfur and organo-chlorine pesticide have it is pungent Peculiar smell.
Parathion (C10H14NO5PS it is) colourless oil liquid, solubility is 24mg/L (25 DEG C) in water.It is slightly soluble in petroleum ether And kerosene, there can be garlic odour with most immiscible organic solvents.It is promptly hydrolyzed in alkaline medium；In neutral or slightly acidic solution In it is relatively stable；It is all unstable to ultraviolet light and air.There are mutagenicity and animal teratogenesis to microorganism.Belong to hypertoxic type, tool There are acute toxicity, irritation, mutagenicity.Human lymphocyte Sister chromatid exohange is influenced when 200 μ g/L；10μmol/L When influence human fibroblasts unscheduled DNA synthesis；When 10 μm of ol/L, rat lymphocyte DNA damage, IARC are made in 16 hours Carcinogenicity is evaluated as G3 grades；The minimum toxic dose of rat oral (TDLo)：360 μ g/kg (pregnant 2~22d/ postpartum 15d) influence new Raw mouse is biochemical and is metabolized；The minimum toxic dose of subcutaneous rat (TDLo)：9800 μ g/kg (pregnant 7~13d), lethal tire.Parathion energy Enter human body by alimentary canal, respiratory tract and complete skin and mucous membrane.Parathion in environment can also be sent out by food chain Raw biological concentration.
Malathion (C10H19O6PS2) it is colourless or pale yellow oily liquid, there is garlic odour taste.Chance open fire, high fever is flammable；With Strong oxidizer reacts；Phosphorus, sulfur oxide gas are generated by thermal decomposition；It is toxic to animal, there is stimulation to eyes, skin Property；Active in 5.0 or less pH, pH7.0 or more is easy hydrolysis failure.Female mice acute oral LD50 is 1751.5mg/kg, male Rat acute LD50 is 1634.5mg/kg, and the percutaneous LD50 of rat acute is 4000~6150mg/kg.Poisoning symptom is head Bitterly, dizziness, nausea, inability, hidrosis, vomiting, salivation, eye-blurred, myosis, spasm, stupor, flesh fibrillation, pulmonary edema etc.. Generally morbidity is very fast after contact in a short time (oral, sucking, skin, mucous membrane) a large amount of organophosphorus pesticides.Malathion poisoning is dived The volt phase is longer.It absorbs through skin, falls ill in 12h, poisoning symptom shows as anorexia, Nausea and vomiting, abdominal pain, hidrosis, thing Object is fuzzy, myosis, respiratory secretions increase, bronchial spasm expiratory dyspnea, pulmonary edema；Sample Symptoms shake for muscle bundle It quivers, muscle spasmus, myoparalysis；Central nervous system symptom shows as headache, dizzy, insomnia drowsiness, weak, irritated, twitch living, dusk Fan, brain edema.The state of an illness is heavier when oral ingestion, and gastrointestinal symptom becomes apparent from.Some cases can have cardiac muscle, liver, renal damage.
Parathion-methyl (C8H10NO5PS)：Colorless crystalline powder, industrial goods are brown or yellow liquid or solid, are met bright Fire, high fever is flammable, by poisonous gas such as thermal decomposition release phosphorus, sulfur oxides, can be decomposed rapidly in lye.To big white mouse urgency Property LD50 of passing through mouth be 14~24mg/kg, to rabbit acute percutaneous LD50 be 300~400mg/kg, belong to high toxic pesticide.Methyl is to sulphur Phosphorus tool is tagged and stomach poison function, can inhibit the vigor of cholinesterase in pest nervous system and lethal, and insecticidal spectrum is wide, is often processed into Missible oil or pulvis (see formulations of pesticide) use, and are mainly used for prevention various agricultural pest.It is prominent with certain cause to microorganism Denaturation, such as 667 μ g/ wares of salmonella typhimurium；Escherichia coli：10mmol/L.Gaseous state, mist state, aerosol state or powdery It is easily absorbed for respiratory tract, liquid can be through eye mucous membrane, skin or gastrointestinal tract.Into after in vivo, rapid distribution to each device of whole body Official organizes, and strong bonded with histone.
Organo-chlorine pesticide is one of current most popular insecticide, is occupied an important position in pesticide market.So And in organo-chlorine pesticide production and agricultural chemicals waste water processing procedure, there is volatile Pesticidal products or intermediate, waved from liquid phase Hair, spills into air, forms air pollution.
Volatile pesticide and intermediate include：Metrifonate, DDVP, phosphorus trichloride, dichlobenil, herbicide, thimet, Phoxim, biphenyl, BFPO, alkane and alkane substitute class intermediate, aromatic intermediate etc. (choosing organochlorine), these substances Most of have a pungent smell, some substances such as diethyl phosphite, trichloroacetaldehyde, belong to carcinogenicity, mutagenicity and The cause property substance of the three of teratogenesis.
It is these organic sulfurs with peculiar smell, organic because being aerated, pushing the mechanical disturbances such as flow in wastewater treatment process Chlorine is spilt into from water in air, forms odor pollution.If not being subject to processing appropriate, the organochlorine in air is spilt into, Serious pollution environment, is detrimental to health.
Biotechnology is effective at present because it has many advantages, such as at low cost, easy to operate, technology cleaning, non-secondary pollution Handle one of the important method containing organic compounds.The principle of biologic treating technique is to utilize the biological oxidation of microorganism will Organic sulfur conversion is the process of the harmless or low harmful substances such as sulfate, carbon dioxide, water, as follows：
Malathion → carbon dioxide+sulfate+water；
Parathion-methyl → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water；
Parathion → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water.
The principle of biologic treating technique is to convert organochlorine to carbon dioxide, salt using the biological oxidation of microorganism Hydrochlorate and water etc. be harmless or the process (formula 1) of low harmful substance.
C2HCl3O+O2→CO2+H2O+HCl (formula 1)
Some researches show that the facultative chemoheterotrophy bacterium in natural environment belongs to pseudomonad (Pseudomonas Sp.) X2 (201310551914.2), Sphingobacterium (Sphingobacterium griseoflavus) SCU-B140 (201510715812.9), Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans), thermophilic iron hook end The mixed culture of spirillum (Leptospirillum ferriphilum) and Ferroplasma acidiphilum (201310745138.X) etc. has degradation to organosulfur pesticide.But it not yet finds for handling organic sulfur and organic The Meng Shi pseudomonads (Pseudomonas monteilii) of chlorine, and use Meng Shi pseudomonads processing sulfur-bearing, contain chlorine pollution The method of object.
Invention content：
In order to solve, volatility organic sulfur, organochlorine treatment effeciency in the prior art be low, microorganism is on mixed carrier surface The problem of uneven, startup is slow, long-time service filler collapses is loaded, the present invention provides one plant of Meng Shi pseudomonad (Pseudomonas monteilii), and the bacterial strain is applied to removing containing in organic sulfur, organochlorine exhaust gas.
The Meng Shi pseudomonads, specially Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, be from It is tamed and dociled in agricultural chemicals waste water containing the organochlorines such as the organic sulfurs such as parathion-methyl, malathion, parathion and phosphorus trichloride, trichloroacetaldehyde Change, isolated separation bacterium.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms on March 10th, 2017 Common micro-organisms center, deposit number are CGMCC No.13748, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Number, Institute of Microorganism, Academia Sinica, postcode 100101.
The bacterial strain feature is as follows：
Meng Shi pseudomonads (Pseudomonas monteilii) LW-1：Cell is in rod-shaped, peritrichous, gram dye Color is negative, and Starch Hydrolysis, VP, indole reaction are feminine gender, and gelatin liquefaction, glucose response are the positive.Bacterium colony is white, round, Surface is smooth, moistens sticky, flat protrusion.In the natural environment, competitiveness is strong, wide adaptation range, can utilize carbon extensively Hydrate, amino acid, organic acid be carbon source, pH wide adaptation ranges (pH 3.0-7.0), under high temperature (50 DEG C -60 DEG C) part all It can grow, optimum growth temperature is 30-55 DEG C, pH 4.0-6.5.
The present invention also provides the microbial inoculum that one kind is prepared by Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, The microbial inoculum can be used for purifying the exhaust gas containing organic sulfur, organochlorine, and it is organic to solve the volatility that the processes such as agricultural chemicals waste water processing generate The preparation method of the pollution problem of sulphur, organochlorine, the microbial inoculum is as follows：
(1) zymotic fluid preparation method
Meng Shi pseudomonads LW-1 is accessed after inclined-plane culture in liquid fermentation medium, step by step by 10-25% inoculum concentrations Amplification culture, fermentation condition：35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen>2.0mg/L, tank press 0.03-0.10Mpa items Under part, incubation time 45-55h；
The liquid fermentation medium is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min, sterilizing postcooling to room temperature add 4.5mg/L parathion and/or trichloroacetaldehyde (when microbial inoculum is used to handle sulphur-containing exhaust gas 4.5mg/L parathion is added；4.5mg/L trichloroacetaldehydes are added when microbial inoculum is for handling chlorine contained exhaust gas；Microbial inoculum is for handling sulfur-bearing With addition 4.5mg/L parathion and 4.5mg/L trichloroacetaldehydes when chlorine contained exhaust gas)；
(2) liquid bacterial agent preparation method
Zymotic fluid is placed in refrigerated centrifuge discards supernatant liquid in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, Precipitation is diluted with sterile nutrient solution, obtains the bacteria suspension that cell concentration is 0.3-0.95g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum products, for volatility organic sulfur and/or The processing of organochlorine exhaust gas；
The sterile nutrient solution is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
The present invention also provides the above-mentioned microbial inoculums of use to handle the method containing organic sulfur and/or organochlorine exhaust gas, specific as follows：
Aforesaid liquid microbial inoculum is supported on filler, for handle the generation of agricultural chemicals waste water reservoir containing organic sulfur and/or having Machine chlorine exhaust gas, gas flow 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, malathion, to sulphur Phosphorus, parathion-methyl total concentration be 20-200mg/m3, and/or trichloroacetaldehyde a concentration of 5-100mg/m3, malathion, right Sulphur phosphorus, parathion-methyl degradation efficiency can reach 90% or more, and trichloroacetaldehyde degradation efficiency can reach 75% or more；
The filler is one or more in polyurethane foamed blocks, haydite, volcanic rock or molecular sieve, and Meng Shi is false in filler Monad LW-1 cell concentrations 1.0 × 105-9.0×108CFU/g(filler)。
The present invention also provides a kind of microbial activity filler, the microbial activity filler is by Meng Shi pseudomonads LW-1 Bacteria suspension is adsorbed on filler, and filler is seated in wire side hollow ball and is prepared, can preferably treatment and purification containing organic The exhaust gas of sulphur and/or organochlorine.
The filler is one or more in polyurethane foamed blocks, haydite, volcanic rock or molecular sieve；
The packing density is 20-80kg/m3, porosity 50-97%, grain size 10-100mm；
The mass ratio of the microbial bacteria suspension and filler is 1-12: 1；
Meng Shi pseudomonad LW-1 cell concentrations 1.0 × 10 in the microbial activity filler5-9.0×108CFU/ g(filler)；
The wire side hollow ball material is polyethylene, polyvinyl chloride or polypropylene；
The hollow bulb diameter 6cm of the wire side, is made of two hemisphere, and each hemisphere surface is porous, the aperture in each hole 2-8mm；
There are a circle reinforcing ring, reinforcing ring to be equipped with lock in the middle part of the wire side hollow ball, convenient for opening and being closed two Hemisphere.
The specific preparation process of the filler is as follows：
(1) preparation of Meng Shi pseudomonads LW-1 bacteria suspensions
1. by liquid fermentation medium is accessed after Meng Shi pseudomonad LW-1 slant activations, carried out by 10-25% inoculum concentrations Expand fermented and cultured step by step；
Fermentation culture conditions are：In 35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen>2.0mg/L, tank press 0.03- Under the conditions of 0.10Mpa, incubation time 45-55h；
The group of the liquid fermentation medium becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.2-7.0,115 DEG C go out Bacterium 20min, sterilizing postcooling to room temperature adds 4.5mg/L parathion and/or trichloroacetaldehyde (adds when for handling sulphur-containing exhaust gas Enter 4.5mg/L parathion；4.5mg/L trichloroacetaldehydes are added when for handling chlorine contained exhaust gas；For handling sulfur-bearing and exhaust gas containing rate When 4.5mg/L parathion and 4.5mg/L trichloroacetaldehydes is added)；
2. after fermented and cultured, zymotic fluid being placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, 10- is centrifuged 20min discards supernatant liquid, and precipitation is diluted with sterile nutrient solution, and acquisition cell concentration is 0.30-0.95g/L Meng Shi pseudomonads LW-1 bacteria suspensions；
The group of the sterile nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.8-7.2,121 DEG C go out Bacterium 20min；
(2) preparation of microbial activity filler：
1. the filler of packet or grain shape is filled into wire side hollow ball, the mass ratio of filler and wire side hollow ball For 0.9-3:1；
2. Meng Shi pseudomonad LW-1 bacteria suspensions prepared by step (1) are sprayed by the hole of wire side hollow ball to packet Or the surface of the filler of grain shape；
3. having the wire side hollow ball of filler to be put into impeller load rotates mixing；
4. again to the wire side hollow ball surface spraying Meng Shi pseudomonad LW-1 bacteria suspensions equipped with filler；
Step 1. -4. 2-3 time is repeated, makes in hollow ball surface and hole that equal appendix has bacterium cell, acquisition Meng Shi on filler Pseudomonad LW-1 cell concentrations reach 1.0 × 105-9.0×108CFU/g(filler)Microbial activity filler, 4 DEG C of preservations are standby With.
The present invention also provides a kind of the side containing organic sulfur and/or organochlorine exhaust gas is handled using mentioned microorganism active filler Method is as follows：
(1) there is the microbial activity filler of Meng Shi pseudomonads LW-1 to be placed in a container above-mentioned appendix, be passed through containing pair Sulphur phosphorus gas (when processing sulphur-containing exhaust gas), using parathion as phosphorus source, sulphur source, activates microbial activity filler；Soak time is 2- 15 days；
A concentration of 50-200mg/m of parathion in the gas containing parathion being passed through3；
It is passed through the gas containing trichloroacetaldehyde when handling chlorine contained exhaust gas, microbial activity filler is activated with trichloroacetaldehyde；It is living It is 2-15 days to change the time；A concentration of 50-200mg/m of the trichloroacetaldehyde3；
It is passed through the gas containing parathion and trichloroacetaldehyde when processing while sulfur-bearing and chlorine contained exhaust gas, activates microbial activity Filler；Soak time is 2-15 days；A concentration of 50-200mg/m of parathion3, a concentration of 50-200mg/m of trichloroacetaldehyde3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through containing organic sulfur and/or chlorine exhaust gas, With the organic sulfur and/or chlorine in Meng Shi pseudomonads LW-1 purification exhaust gas, microbial activity packing volume is 0.1-1000m3, have The total concentration of machine sulphur is 20-250mg/m3And/or the total concentration of organochlorine is 5-100mg/m3, gas flow rate 0.05- 10000m3/ h, residence time 1.5-10.0min, purification temperature are 30-65 DEG C；Periodically to microbial activity filler surface spraying It is to spray 1 time for 1-3 days that nutrient solution, which maintains the activity of microbial activity filler, spray frequency, and each spray rate is 1.0-200L/ Min, each spray time are 10-90min；
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, pH value 6.2-7.0.
Advantageous effect：
1. Meng Shi pseudomonads (Pseudomonas monteilii) LW-1 of the present invention has higher degradation organic sulfur With the efficiency of organochlorine, to the degradation efficiency of organic sulfur up to 90-100%, to the degradation efficiency of organochlorine up to 75% or more； And the laundering period of dominant bacteria is short, and inoculation can quickly start degradation reaction in 1 week, applies the exhaust gas life containing organic sulfur in purification In object reactor, the starting period can be shortened.
2. using the effect of a variety of mechanics such as sedimentation, attachment and centrifugation, by the Meng Shi pseudomonads (Pseudomonas that degrades Monteilii) LW-1 appendix form microbial activity filler, bacterium cell on the light porous material of wire side hollow ball inside It can not only be uniformly adhered to filler surface, and can be entered in the hole inside filler, the bacterium of microbial activity filler is made Cell appendix amount is high, is not easy to be lost in from bioreactor.
3. Meng Shi pseudomonads (Pseudomonas monteilii) LW-1 has, distribution is wide, quantity is more, nutritional need is simple Feature single, breeding is fast, competition colonization power is strong.The pseudomonad inhibits other microorganisms, containing biting iron by secreting antibiotic Element, the siderophore that can be secreted have a clear superiority in the competition of iron.
4. Meng Shi pseudomonads (Pseudomonas monteilii) LW-1 is chemoorganotrophy type, there is catalase, It can utilize and decomposing volatile organic sulfur/organochlorine.
5. Meng Shi pseudomonads (Pseudomonas monteilii) LW-1 has antiviral activity, to TMV (Tobacco mosaics Virus), the viruses such as PVY (marmor upsilon) have inhibiting effect, use safer.
6. the rough surface, porous of the carrier material for microbial activity filler is suitable for bacterium cell and adheres to；Specific surface area Greatly, be conducive to the volatility such as bacterium cell and parathion, trichloroacetaldehyde organic sulfur, organochlorine contacts；Load the wire side of light filler Hollow ball hole is more, and good permeability, resistance is small, and mass-transfer efficiency is high；Microbial activity filler weight is light, intensity is high, the pressure loss It is small；It is high temperature resistant, corrosion-resistant；Not perishable deformation, can be used for a long time and adapt to the physical condition of Various Complex.
Description of the drawings：
Fig. 1 Meng Shi pseudomonads (Pseudomonas monteilii) LW-1 phylogenetic trees.
Specific implementation mode：
In order to make the object, technical solution and advantage of this patent be more clearly understood, below in conjunction with specific embodiment, to this Patent is further elaborated.It should be appreciated that specific embodiment described herein is only to explain this patent, and do not have to It is of the invention in limiting.
Strain idenfication：
DNA is extracted：It is carried using Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and respective environment sample DNA The bacterial strain DNA that purification kit (Taiwan dot nanometer technical concern Co., Ltd) obtains screening is taken to extract and purify, Bacterial universal primers F16S-27 and R16S-1492 is selected to carry out PCR amplification, primer sequence is respectively：
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction conditions are set as：94 DEG C of pre-degeneration 5min；Subsequent 94 DEG C of denaturation 30s, 56 DEG C of annealing 30s, 72 DEG C of extensions 90s recycles 25 periods；Then 72 DEG C of extension 10min；Last 4 DEG C of holdings 10min.By being purified to PCR product, after quality inspection PCR purified products are connected on PMD-18T carriers, are then transformed into bacillus coli DH 5 alpha competent cell.After converting 50 μ L of competent cell be coated on the LB culture mediums containing isopropylthiogalactoside, X-gal and ammonia benzyl.37 DEG C of constant temperature Incubator is incubated overnight, and LB liquid medium culture is carried out from 25 white single bacterium spots of each tablet picking.After culture 2 hours, Culture solution is subjected to sequencing analysis.The sequence measured uses DNAMAN softwares to carry out similitude merger first, and similitude is more than 97% sequence merger is an operating unit, and the representative sequence chosen in each operating unit utilizes BLAST and GenBank numbers Homology analysis is carried out according to the 16SrDNA gene orders in library, 1400bp or more length is chosen and is compared (Clustelx1.83), Phylogenetic Analysis (MEGA3.1), system are carried out using ortho position connection (Neighbour Joining) method Development tree is as shown in Figure 1.It is compared by DNA sequence dna, which is Meng Shi pseudomonads (Pseudomonas monteilii), It is named as Meng Shi pseudomonads (Pseudomonas monteilii) LW-1.
Embodiment 1 is a kind of for purifying the microbial inoculum containing the organic sulphur waste gas
The microbial inoculum that one kind is prepared by Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum Preparation method it is as follows：
(1) zymotic fluid preparation method
Meng Shi pseudomonads LW-1 accesses fermentation medium after inclined-plane culture, expands culture step by step by 10% inoculum concentration, In 35 DEG C, ventilation quantity 10L/min, dissolved oxygen>2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, incubation time 55h must ferment Liquid；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The fermentation medium is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min, goes out Bacterium postcooling to room temperature adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 2000rpm, centrifugal concentrating 20min, discards supernatant liquid, precipitation nothing Bacterial nutrient solution dilutes, and obtains the bacteria suspension that cell concentration is 0.3g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum products, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on polyurethane foamed blocks filler, Meng Shi pseudomonads LW-1 cell concentrations in filler 1.0×105CFU/g(filler), for handle agricultural chemicals waste water reservoir generation contain the organic sulphur waste gas, gas flow 100m3/ h, stops Stay time 1.0min, 35 DEG C of temperature, a concentration of 13.2mg/m of malathion3, parathion a concentration of 33.7mg/m3, methyl pair A concentration of 8.3mg/m of sulphur phosphorus3, malathion removal rate is 92.6%, parathion degradation efficiency is 85.1%, parathion-methyl Degradation efficiency can reach 90.7%.
Embodiment 2 is a kind of for purifying the microbial inoculum containing the organic sulphur waste gas
The microbial inoculum that one kind is prepared by Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum Preparation method it is as follows：
(1) zymotic fluid preparation method
Meng Shi pseudomonads LW-1 is accessed after inclined-plane culture in fermentation medium, amplifies training step by step by 25% inoculum concentration It supports, in 45 DEG C, ventilation quantity 100L/min, dissolved oxygen>2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, incubation time 45h is obtained Zymotic fluid；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The fermentation medium is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min, goes out Bacterium postcooling to room temperature adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 8000rpm, centrifugal concentrating 5min, discards supernatant liquid, precipitation nothing Bacterial nutrient solution dilutes, and obtains the bacteria suspension that cell concentration is 0.8g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum products, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on ceramics filling, Meng Shi pseudomonads LW-1 cell concentrations 9.0 in filler × 108CFU/g(filler), for handle agricultural chemicals waste water reservoir generation contain the organic sulphur waste gas, gas flow 5000m3/ h, when stop Between 5.0min, 55 DEG C of temperature, malathion, parathion, parathion-methyl total concentration be 132.5mg/m3, malathion, right Sulphur phosphorus, parathion-methyl degradation efficiency can reach 91.4%.
Embodiment 3 is a kind of for purifying the microbial inoculum containing the organic sulphur waste gas
The microbial inoculum that one kind is prepared by Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum Preparation method it is as follows：
(1) zymotic fluid preparation method
Meng Shi pseudomonads LW-1 is accessed after kind of inclined-plane culture in fermentation medium, amplifies training step by step by 18% inoculum concentration It supports, fermentation condition：In 40 DEG C, ventilation quantity 50L/min, dissolved oxygen>2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, when culture Between 50h obtain zymotic fluid；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The fermentation medium is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min, goes out Bacterium postcooling to room temperature adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, discards supernatant liquid, precipitation nothing Bacterial nutrient solution dilutes, and obtains the bacteria suspension that cell concentration is 0.6g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum products, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution is：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on volcanic filling material, Meng Shi pseudomonads LW-1 cell concentrations 5.0 in filler × 107CFU/g(filler), for handle agricultural chemicals waste water reservoir generation contain the organic sulphur waste gas, gas flow 2500m3/ h, when stop Between 3.0min, temperature 45 C, malathion, parathion, parathion-methyl concentration be respectively 16.3mg/m3, 20.8mg/m3With 6.5mg/m3, malathion, parathion, parathion-methyl degradation efficiency can respectively reach 89.2%, 93.6% and 92.5%.
A kind of microbial inoculum for purifying the exhaust gas containing organochlorine of embodiment 1 '
Parathion in step (1) fermentation medium is only replaced with the trichlorine of equivalent by bacterial preparation process with embodiment 1 Acetaldehyde.
The method that the exhaust gas containing organochlorine is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on polyurethane foamed blocks filler, Meng Shi pseudomonads LW-1 cell concentrations in filler 5.0×106CFU/g(filler), the exhaust gas containing organochlorine for handling the generation of agricultural chemicals waste water reservoir, gas flow 100m3/ h, stops Stay time 1.0min, 35 DEG C of temperature, a concentration of 42.6mg/m of trichloroacetaldehyde3, trichloroacetaldehyde degradation efficiency can reach 80.3%.
A kind of microbial inoculum for purifying the exhaust gas containing organochlorine of embodiment 2 '
Parathion in step (1) fermentation medium is only replaced with three chloroethene of equivalent by bacterial preparation process with embodiment 2 Aldehyde.
The method that the exhaust gas containing organochlorine is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on ceramics filling, Meng Shi pseudomonads LW-1 cell concentrations 9.0 in filler × 108CFU/g(filler), the exhaust gas containing organochlorine for handling the generation of agricultural chemicals waste water reservoir, gas flow 5000m3/ h, when stop Between 5.0min, 55 DEG C of temperature, a concentration of 20.2mg/m of trichloroacetaldehyde3, the concentration of trichloroacetaldehyde is reduced to 3.6mg/ after processing m3, degradation efficiency can reach 82.2%.
A kind of microbial inoculum for purifying the exhaust gas containing organochlorine of embodiment 3 '
Parathion in step (1) fermentation medium is only replaced with the trichlorine of equivalent by bacterial preparation process with embodiment 3 Acetaldehyde.
The method that the exhaust gas containing organochlorine is handled using above-mentioned microbial inoculum, it is specific as follows：
Aforesaid liquid microbial inoculum is supported on volcanic filling material, Meng Shi pseudomonads LW-1 cell concentrations 4.0 in filler × 108CFU/g(filler), the exhaust gas containing organochlorine for handling the generation of agricultural chemicals waste water reservoir, gas flow 2500m3/ h, when stop Between 3.0min, temperature 45 C, a concentration of 61.9mg/m of trichloroacetaldehyde3, trichloroacetaldehyde degradation efficiency can reach 76.5%.
Embodiment 4：A kind of microbe stuffing of the removing containing the organic sulphur waste gas and its preparation
A kind of microbial activity filler is to be adsorbed in polyurethane foamed blocks filler by Meng Shi pseudomonad LW-1 bacteria suspensions On, and filler is seated in wire side hollow ball and is prepared；
Packing density is 20kg/m3, porosity 90%, grain size 10mm；The mass ratio of microbial bacteria suspension and filler It is 1: 1；Meng Shi pseudomonads LW-1 cell concentrations 1.0 × 10 in microbial activity filler5CFU/g(filler)；
The wire side hollow ball material is polyethylene；The hollow bulb diameter 6cm of wire side, is made of, Mei Geban two hemisphere Ball surface is porous, the aperture 2mm in each hole；There is a circle reinforcing ring in the middle part of wire side hollow ball, reinforcing ring is equipped with lock, convenient for opening Open and be closed two hemisphere.
The specific preparation process of the filler is as follows：
(1) preparation of Meng Shi pseudomonads LW-1 bacteria suspensions
1. by liquid fermentation medium is accessed after Meng Shi pseudomonad LW-1 slant activations, carried out step by step by 10% inoculum concentration Expand fermented and cultured；
Fermentation culture conditions are：In 35 DEG C, ventilation quantity 10L/min, dissolved oxygen>2.0mg/L, tank press 0.03-0.10Mpa items Under part, incubation time 55h；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The group of the liquid fermentation medium becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.2-7.0,115 DEG C go out Bacterium 20min, sterilizing postcooling to room temperature add 4.5mg/L parathion；
2. after fermented and cultured, zymotic fluid being placed in refrigerated centrifuge in 4 DEG C, 2000rpm, 10min is centrifuged, discards Supernatant, precipitation are diluted with sterile nutrient solution, and acquisition cell concentration is 0.30g/L Meng Shi pseudomonad LW-1 bacteria suspensions；
The group of the sterile nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.8-7.2,121 DEG C go out Bacterium 20min；
(2) preparation of microbial activity filler：
1. the filler of packet is filled into wire side hollow ball, the mass ratio of filler and wire side hollow ball is 0.9：1；
2. Meng Shi pseudomonad LW-1 bacteria suspensions prepared by step (1) are sprayed by the hole of wire side hollow ball to packet The surface of the filler of shape；
3. having the wire side hollow ball of filler to be put into impeller load rotates mixing；
4. again to the wire side hollow ball surface spraying Meng Shi pseudomonad LW-1 bacteria suspensions equipped with filler；
1. -4. 2 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, obtain Meng Shi vacations Monad LW-1 cell concentrations reach 1.0 × 105CFU/g(filler)Microbial activity filler, 4 DEG C save backup.
Embodiment 5：A kind of microbe stuffing of the removing containing the organic sulphur waste gas and its preparation
A kind of microbial activity filler is to be adsorbed on ceramics filling by Meng Shi pseudomonad LW-1 bacteria suspensions, and will fill out Material, which is seated in wire side hollow ball, to be prepared；Packing density is 80kg/m3, porosity 50%, grain size 100mm；Micro- life The mass ratio of object bacteria suspension and filler is 12: 1；Meng Shi pseudomonads LW-1 cell concentrations 9.0 in microbial activity filler × 108CFU/g(filler)；
The wire side hollow ball material is polyvinyl chloride；The hollow bulb diameter 6cm of wire side, is made of, each two hemisphere Hemisphere surface is porous, the aperture 8mm in each hole；There are a circle reinforcing ring, reinforcing ring to be equipped with lock, be convenient in the middle part of wire side hollow ball Open and be closed two hemisphere.
The specific preparation process of the filler is as follows：
(1) preparation of Meng Shi pseudomonads LW-1 bacteria suspensions
1. by liquid fermentation medium is accessed after Meng Shi pseudomonad LW-1 slant activations, carried out step by step by 25% inoculum concentration Expand fermented and cultured；
Fermentation culture conditions are：In 45 DEG C, ventilation quantity 100L/min, dissolved oxygen>2.0mg/L, tank press 0.10Mpa conditions Under, incubation time 45h；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The group of the liquid fermentation medium becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.2-7.0,115 DEG C go out Bacterium 20min, sterilizing postcooling to room temperature add 4.5mg/L parathion；
2. after fermented and cultured, zymotic fluid being placed in refrigerated centrifuge in 4 DEG C, 8000rpm, 20min is centrifuged, discards Supernatant, precipitation are diluted with sterile nutrient solution, and acquisition cell concentration is 0.95g/L Meng Shi pseudomonad LW-1 bacteria suspensions；
The group of the sterile nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.8-7.2,121 DEG C go out Bacterium 20min；
(2) preparation of microbial activity filler：
1. the filler of grain shape is filled into wire side hollow ball, the mass ratio of filler and wire side hollow ball is 2：1；
2. Meng Shi pseudomonad LW-1 bacteria suspensions prepared by step (1) are sprayed by the hole of wire side hollow ball to granulated The surface of the filler of shape；
3. having the wire side hollow ball of filler to be put into impeller load rotates mixing；
4. again to the wire side hollow ball surface spraying Meng Shi pseudomonad LW-1 bacteria suspensions equipped with filler；
1. -4. 3 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, obtain Meng Shi vacations Monad LW-1 cell concentrations reach 9.0 × 108CFU/g(filler)Microbial activity filler, 4 DEG C save backup.
Embodiment 6：A kind of microbe stuffing of the removing containing the organic sulphur waste gas and its preparation
A kind of microbial activity filler is to be adsorbed on volcanic filling material by Meng Shi pseudomonad LW-1 bacteria suspensions, and incite somebody to action Filler is seated in wire side hollow ball and is prepared；Packing density is 50kg/m3, porosity 75%, grain size 50mm；Micro- life The mass ratio of object bacteria suspension and filler is 8: 1；Meng Shi pseudomonads LW-1 cell concentrations 3.0 in microbial activity filler × 107CFU/g(filler)；
The wire side hollow ball material is polypropylene；The hollow bulb diameter 6cm of wire side, is made of, Mei Geban two hemisphere Ball surface is porous, the aperture 5mm in each hole；There is a circle reinforcing ring in the middle part of wire side hollow ball, reinforcing ring is equipped with lock, convenient for opening Open and be closed two hemisphere.
The specific preparation process of the filler is as follows：
(1) preparation of Meng Shi pseudomonads LW-1 bacteria suspensions
1. by liquid fermentation medium is accessed after Meng Shi pseudomonad LW-1 slant activations, carried out step by step by 17% inoculum concentration Expand fermented and cultured；
Fermentation culture conditions are：In 40 DEG C, ventilation quantity 50L/min, dissolved oxygen>2.0mg/L, tank press 0.03-0.10Mpa items Under part, incubation time 50h；
Slant medium group becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The group of the liquid fermentation medium becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.2-7.0,115 DEG C go out Bacterium 20min, sterilizing postcooling to room temperature add 4.5mg/L parathion；
2. after fermented and cultured, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, 10-20min is centrifuged, Liquid is discarded supernatant, precipitation is diluted with sterile nutrient solution, and acquisition cell concentration is 0.65g/L Meng Shi pseudomonad LW-1 bacteria suspensions；
The group of the sterile nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO4 0.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH value 6.8-7.2,121 DEG C go out Bacterium 20min；
(2) preparation of microbial activity filler：
1. the filler of packet is filled into wire side hollow ball, the mass ratio of filler and wire side hollow ball is 3：1；
2. Meng Shi pseudomonad LW-1 bacteria suspensions prepared by step (1) are sprayed by the hole of wire side hollow ball to box-shaped The surface of the filler of shape；
3. having the wire side hollow ball of filler to be put into impeller load rotates mixing；
4. again to the wire side hollow ball surface spraying Meng Shi pseudomonad LW-1 bacteria suspensions equipped with filler；
1. -4. 3 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, obtain Meng Shi vacations Monad LW-1 cell concentrations reach 3.0 × 107CFU/g(Filler)Microbial activity filler, 4 DEG C save backup.
A kind of microbe stuffing of the removing exhaust gas containing organochlorine of embodiment 4 ' and its preparation
Parathion in preparation process step (1) is only replaced with the trichlorine of equivalent by filler and preparation method with embodiment 4 Acetaldehyde.
A kind of microbe stuffing of the removing exhaust gas containing organochlorine of embodiment 5 ' and its preparation
Parathion in preparation process step (1) is only replaced with the trichlorine of equivalent by filler and preparation method with embodiment 5 Acetaldehyde.
A kind of microbe stuffing of the removing exhaust gas containing organochlorine of embodiment 6 ' and its preparation
Parathion in preparation process step (1) is only replaced with the trichlorine of equivalent by filler and preparation method with embodiment 6 Acetaldehyde.
Embodiment 7：A kind of method of the microbial activity filler processing containing the organic sulphur waste gas
The method containing the organic sulphur waste gas being handled using microbial activity filler described in embodiment 4 the present invention also provides a kind of, It is as follows：
(1) there is the microbial activity filler of Meng Shi pseudomonads LW-1 to be placed in a container 4 appendix of embodiment, be passed through and contain The gas of parathion activates microbial activity filler using parathion as phosphorus source, sulphur source；Soak time is 15 days；
A concentration of 50mg/m of parathion in the gas containing parathion being passed through3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through containing the organic sulphur waste gas, with Meng Shi vacations Organic sulfur in monad LW-1 purification exhaust gas, microbial activity packing volume is 0.1m3, malathion and parathion in air inlet Concentration be respectively 17.3mg/m3And 46.7mg/m3, gas flow rate 0.05m3/ h, residence time 1.5min, purification temperature are 30℃；It is 3 days that the activity of microbial activity filler, spray frequency are periodically maintained to microbial activity filler surface spraying nutrient solution Spray 1 time, each spray rate are 1.0L/min, and each spray time is 10min；Gas outlet malathion and parathion it is dense Degree is reduced to 2.2mg/m3And 4.3mg/m3, removal rate respectively reaches 87.3% and 90.8%.
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, pH value 6.2-7.0.
Embodiment 8：A kind of method of the microbial activity filler processing containing the organic sulphur waste gas
The method containing the organic sulphur waste gas being handled using microbial activity filler described in embodiment 5 the present invention also provides a kind of, It is as follows：
(1) there is the microbial activity filler of Meng Shi pseudomonads LW-1 to be placed in a container 5 gained appendix of embodiment, lead to Enter the gas containing parathion, using parathion as phosphorus source, sulphur source, activates microbial activity filler；Soak time is 2 days；
A concentration of 200mg/m of parathion in the gas containing parathion being passed through3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through containing the organic sulphur waste gas, with Meng Shi vacations Organic sulfur in monad LW-1 purification exhaust gas, microbial activity packing volume is 1000m3, in air inlet, parathion it is a concentration of 162.7mg/m3, a concentration of 32.1mg/m of parathion-methyl3, gas flow rate 10000m3/ h, residence time 10.0min, only It is 65 DEG C to change temperature；The activity of microbial activity filler, spray are periodically maintained to microbial activity filler surface spraying nutrient solution Frequency is to spray 1 time for 1 day, and each spray rate is 200L/min, and each spray time is 90min；Gas outlet, parathion it is dense Degree is reduced to 11.3mg/m3, the concentration of parathion-methyl is reduced to 2.8mg/m3.It is (right that their removal rate respectively reaches 93.1% Sulphur phosphorus) and 91.3% (parathion-methyl).
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, pH value 6.2-7.0.
Embodiment 9：A kind of method of the microbial activity filler processing containing the organic sulphur waste gas
The method containing the organic sulphur waste gas being handled using microbial activity filler described in embodiment 6 the present invention also provides a kind of, It is as follows：
(1) there is the microbial activity filler of Meng Shi pseudomonads LW-1 to be placed in a container 6 gained appendix of embodiment, lead to Enter the gas containing parathion, using parathion as phosphorus source, sulphur source, activates microbial activity filler；Soak time is 7 days；
A concentration of 100mg/m of parathion in the gas containing parathion being passed through3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through containing the organic sulphur waste gas, with Meng Shi vacations Organic sulfur in monad LW-1 purification exhaust gas, microbial activity packing volume is 500m3, in air inlet, the concentration of malathion For 179.2mg/m3, a concentration of 56.3mg/m of parathion-methyl3, gas flow rate 5000m3/ h, residence time 5.0min, only It is 50 DEG C to change temperature；The activity of microbial activity filler, spray are periodically maintained to microbial activity filler surface spraying nutrient solution Frequency is to spray 1 time for 2 days, and each spray rate is 100L/min, and each spray time is the gas outlets 50min., malathion Concentration is reduced to 13.9mg/m3, the concentration of parathion-methyl is reduced to 4.1mg/m3.The removal of malathion and parathion-methyl Rate respectively reaches 92.2% and 92.7%.
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, pH value 6.2-7.0.
A kind of method of microbial activity filler processing exhaust gas containing organochlorine of embodiment 7 '
The present invention also provides a kind of method handling the exhaust gas containing organochlorine using the microbial activity filler of embodiment 4 ', Specific steps will only be replaced for activating the gas of microbial activity filler by containing parathion gas with embodiment 7 in step (1) For the gas containing trichloroacetaldehyde, concentration is constant；To be passed through in step (2) replaced with containing the organic sulphur waste gas it is a concentration of containing a concentration of 37.4mg/m3The exhaust gas of trichloroacetaldehyde.
Gas outlet, trichloroacetaldehyde concentration are reduced to 6.2mg/m3, the removal rate of trichloroacetaldehyde reaches 83.4%.
A kind of method of microbial activity filler processing exhaust gas containing organochlorine of embodiment 8 '
The present invention also provides a kind of method handling the exhaust gas containing organochlorine using the microbial activity filler of embodiment 5 ', Specific steps will only be replaced for activating the gas of microbial activity filler by containing parathion gas with embodiment 8 in step (1) For the gas containing trichloroacetaldehyde, concentration is constant；To be passed through in step (2) replaced with containing the organic sulphur waste gas it is a concentration of containing a concentration of 97.6mg/m3The exhaust gas of trichloroacetaldehyde.
Gas outlet, trichloroacetaldehyde concentration are reduced to 21.3mg/m3, the removal rate of trichloroacetaldehyde reaches 78.2%.
A kind of method of microbial activity filler processing exhaust gas containing organochlorine of embodiment 9 '
The present invention also provides a kind of method handling the exhaust gas containing organochlorine using the microbial activity filler of embodiment 6 ', Specific steps will only be replaced for activating the gas of microbial activity filler by containing parathion gas with embodiment 9 in step (1) For the gas containing trichloroacetaldehyde, concentration is constant；To be passed through in step (2) replaced with containing the organic sulphur waste gas it is a concentration of containing a concentration of 13.5mg/m3The exhaust gas of trichloroacetaldehyde.Gas outlet, trichloroacetaldehyde concentration are reduced to 2.7mg/m3, the removal rate of trichloroacetaldehyde reaches 80%.
Embodiment 10：Experiment effect
6 gained filler of embodiment is used for the processing of following gases：
(1) exhaust gas containing parathion, gas flow 300m are handled3/ h, residence time 100s, purification temperature 30 ℃.Bioreactor continuous operation 2 months, the mean air entry of pH 4-7, parathion are a concentration of：25mg/m3, a concentration of 2mg/ of outlet m3, removal rate reaches 92%.
Contrast and experiment is shown：When ferro element is not added in nutrient solution, after reactor is run 2 months, Meng Shi vacation unit cells Bacterium LW-1 quantity is from 3.0 × 107CFU/g increases to 7.0 × 108CFU/g.It is added containing 0.02g/L iron members when to bioreactor After the nutrient solution of element, after reactor is run 2 months, Meng Shi pseudomonad LW-1 quantity is by 3.0 × 107CFU/g increases to 1.0 × 109CFU/g increases significantly, bioreactor is enable effectively to remove the organic sulfur in exhaust gas.
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO4 0.5g, KH2PO4 1.5g, (NH4) 2SO4 0.5g, NaCl 0.5g, MgSO4 0.2g, CaCl2 0.05g, FeSO4 0.02g, water 1000ml, pH value 6.2-7.0；Spray frequency Rate is to spray 1 time for 2 days, and each spray rate is 200L/min, and each spray time is 50min.
(2) it is used to handle the exhaust gas containing malathion of agricultural chemicals waste water processing procedure generation, a concentration of 10mg/ of malathion m3, gas flow 3000m3/ h, residence time 95s, 30 DEG C of gas temperature, regular replenishment contains the battalion of 0.02g/L ferro elements Nutrient solution (is same as above), and the removal rate of malathion is close to 100%.
Equipment is run more than 3 months, pH 4-6, Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 quantity Maintain 2.0 × 107-2.5×108CFU/g, while reaching purification purpose, equipment can be run steadily in the long term.
(3) exhaust gas containing methyl mercaptan is handled, gas flow 10L/min, residence time 90s are operated under the conditions of 30 DEG C. Bioreactor continuous operation October, the inlet gas concentration of pH4-6, methyl mercaptan are：2-5mg/m3, go out gas concentration and be less than 0.1mg/m3, Average removal rate：98%.Reactor Nei Mengshi pseudomonads (Pseudomonas monteilii) LW-1 quantity be 2.0 × 107-2.5×108CFU/g, bioreactor are stable.
In above-mentioned gas processing procedure, the starting period of bioreactor is one week.And under equal conditions, it will be in filler After strain replaces with the activated sludge of processing agricultural chemicals waste water, the starting period is 2 weeks-January.
Embodiment 10 '：Experiment effect
6 ' gained filler of embodiment is used for the processing of following gases：
Handle the exhaust gas containing trichloroacetaldehyde, gas flow 300m3/ h, residence time 100s, purification temperature are 30 DEG C. Bioreactor continuous operation August, the mean air entry of pH 4-6, trichloroacetaldehyde are a concentration of：30mg/m3, outlet is a concentration of 4.5mg/m3, removal rate reaches 85%.
Contrast and experiment is shown：When ferro element is not added in nutrient solution, after reactor is run 1 month, Meng Shi vacation unit cells Bacterium LW-1 quantity is from 3.0 × 107CFU/g increases to 7.0 × 107CFU/g.It is added containing 0.02g/L iron members when to bioreactor After the nutrient solution of element, after reactor is run 2 months, Meng Shi pseudomonad LW-1 quantity is by 3.0 × 107CFU/g increases to 3.0 × 108CFU/g increases the organochlorine significantly enable in the permanently effective removing exhaust gas of bioreactor.
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO4 0.5g, KH2PO4 1.5g, (NH4) 2SO4 0.5g, NaCl 0.5g, MgSO4 0.2g, CaCl2 0.05g, FeSO4 0.02g, water 1000ml, pH value 6.2-7.0；Spray frequency Rate is to spray 1 time for 2 days, and each spray rate is 200L/min, and each spray time is 50min.
(2) it is used to handle the exhaust gas containing trichloroacetaldehyde of agricultural chemicals waste water processing procedure generation, a concentration of 10mg/ of trichloroacetaldehyde m3, gas flow 3000m3/ h, residence time 95s, 30 DEG C of gas temperature, regular replenishment contains the battalion of 0.02g/L ferro elements Nutrient solution (is same as above), the removal rate 90% of trichloroacetaldehyde, and discharge gas reaches the atmosphere pollutants emission standards of country's formulation.
Equipment is run more than 3 months, pH 4-6, Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 quantity Maintain 7.0 × 106-7.5×107CFU/g, while reaching purification purpose, equipment can be run steadily in the long term.
In above-mentioned gas processing procedure, the starting period of bioreactor is one week.And under equal conditions, it will be in filler After strain replaces with the activated sludge of processing agricultural chemicals waste water, the starting period is 2 weeks-January.
The processing of 11 mixed gas of embodiment
Microbial activity filler is prepared using 6 same method of embodiment and (fermentation medium is only added into 4.5mg/L to sulphur Phosphorus replaces with while adding 4.5mg/L parathion and 4.5mg/L trichloroacetaldehydes) method of the processing containing organic sulfur, chlorine exhaust gas, specifically Steps are as follows：
(1) there is the microbial activity filler of Meng Shi pseudomonads LW-1 to be placed in a container 6 gained appendix of embodiment, lead to Enter the gas containing parathion, trichloroacetaldehyde, activates microbial activity filler；Soak time is 7 days；
A concentration of 100mg/m of parathion in the gas containing parathion being passed through3, trichloroacetaldehyde it is a concentration of 100mg/m3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through containing organic sulfur, chlorine exhaust gas, with illiteracy Organic sulfur, chlorine in family name pseudomonad LW-1 purification exhaust gas, microbial activity packing volume is 500m3, in air inlet, malathion 37.7mg/m3, parathion 16.1mg/m3Trichloroacetaldehyde 9.8mg/m3；Gas flow rate is 5000m3/ h, residence time 5.0min, only It is 50 DEG C to change temperature；The activity of microbial activity filler, spray are periodically maintained to microbial activity filler surface spraying nutrient solution Frequency is to spray 1 time for 2 days, and each spray rate is 100L/min, and each spray time is the gas outlets 50min., malathion 3.2mg/m3, parathion 1.3mg/m3Trichloroacetaldehyde 2.3mg/m3.Malathion removal rate 91.5%, parathion removal rate 92% Trichloroacetaldehyde removal rate 76.5%..
The group of the nutrient solution becomes：Glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, pH value 6.2-7.0.

Claims (2)

1. one plant of Meng Shi pseudomonad, which is characterized in that the Meng Shi pseudomonads are specially Meng Shi pseudomonads (Pseudomonas monteilii) LW-1, deposit number are CGMCC No.13748.
2. the application of Meng Shi pseudomonads described in claim 1.
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