CN108452667B - A kind of biological active filling material comprising the short shape bacillus of Nie Shi - Google Patents
A kind of biological active filling material comprising the short shape bacillus of Nie Shi Download PDFInfo
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- CN108452667B CN108452667B CN201810456580.3A CN201810456580A CN108452667B CN 108452667 B CN108452667 B CN 108452667B CN 201810456580 A CN201810456580 A CN 201810456580A CN 108452667 B CN108452667 B CN 108452667B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/68—Halogens or halogen compounds
- B01D53/70—Organic halogen compounds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention belongs to field of environmental improvement, and in particular to a kind of microbial activity filler containing the short shape bacillus of Nie Shi.The short shape bacillus of Nie Shi is specially the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-1, and deposit number is CGMCC No.13747.The efficiency of microbial activity filler containing the short shape bacillus NY-1 of the Nie Shi degradation organochlorine with higher, up to 90-96%；And the laundering period of dominant bacteria is short, can quickly start degradation reaction in 1 week, applies in exhaust gas bioreactor of the purification containing organochlorine, can shorten the starting period.
Description
Technical field:
The invention belongs to field of environmental improvement, and in particular to the short shape bacillus (Brachybacterium of one plant of Nie Shi
Nesterenkovii) the biological active filling material of NY-1.
Background technique:
Organo-chlorine pesticide be for prevent and treat in the constituent of plant pest contain organic chlorine element organic compound,
It is generally acknowledged critically important a kind of persistence organic pollutant.In organo-chlorine pesticide production and agricultural chemicals waste water treatment process, tool
There are volatile Pesticidal products or intermediate, volatilize, spilt into air from liquid phase, forms air pollution.Volatile pesticide
And intermediate includes: metrifonate, DDVP, phosphorus trichloride, DDT, trichloroacetaldehyde urea, sodium trichloroacetate, six six six, Emhorn
Agent, Niran, endrin, heptachlor, toxaphene, hexachloro-benzene, mirex, trichloroacetaldehyde etc., most of these substances have pungent
Smell, some substances such as trichloroacetaldehyde, belong to carcinogenicity, mutagenicity and teratogenesis three cause property substances.
The colourless volatile oily liquids of trichloroacetaldehyde, irritant smell are 97.8 DEG C of boiling point, -57.5 DEG C of fusing point, relatively close
Degree 1.5121, refractive index 1.4580 is dissolved in water, ethyl alcohol, ether and chloroform, trichloroacetaldehyde hydrate is generated with hydrate, with second
Alcohol is the mixture that raw material reacts generation trichloroacetaldehyde at 80~90 DEG C with chlorine and alcohol closes trichloroacetaldehyde, then anti-with the concentrated sulfuric acid
It answers, is made through distillation, be used as organic synthesis raw material.Such as manufacturing DDT, metrifonate, DDVP insecticide and trichlorine
Acetaldehyde carbamide weedicide, not only causes to seriously affect to ecological environment, but also has high risks to human health.It is chronically at easily
In the organo-chlorine pesticide environment of volatilization, can cause headache, it is dizzy, furious it is congested, shed tears keep in dark place, cough, pharyngalgia, it is out of strength, perspire,
Salivation, nausea, loss of appetite, insomnia and Head And Face cacesthesia etc., moderate intoxication person also vomits in addition to having with above-mentioned symptom
It spits, abdominal pain, has aches in the limbs, twitches, cyanosis, expiratory dyspnea, tachycardia etc.；Severe intoxication person obviously aggravates except above-mentioned symptom
Outside, still there are high fever, hidrosis, contraction of muscle, epilepsy seizure, or even dead.
Currently, the processing technique of the exhaust gas of the volatile organic matter containing organochlorine includes absorbing processing technique, catalysis burning
Processing technique, liquid absorption processing technique, biologic treating technique, Treatment by Photocatalysis Oxidation technology, low-temperature plasma processing technique,
Membrane-base absorption technology etc..Compared with other physical chemistry processing methods, bioremediation has high treating effect, investment and fortune
Row expense is low, safety is good, without secondary pollution, the advantages that being easily managed.Its principle is the biological oxidation using microorganism
CO is converted by organochlorine2、 H2O, chlorate, hydrogen ion and other (formulas 1).
In exhaust-gas treatment bioreactor, certain thickness filler is usually loaded as microorganism carrier, makes micro- life
The biomembrane that object is formed can be attached on filler particles, and can also provide necessary nutrition to microorganism.Biologic packing material
The factors such as material, the size of specific surface area, gas distribution performance, intensity and density directly affect the treatment effeciency and biological respinse of exhaust gas
The operational reliability of device.The type of filler has very much, can be divided into organic filler and inorganic filler.Organic filler include polyurethane,
Polyethylene, composting material etc.；Inorganic filler includes active carbon, haydite, volcanic rock, perlite etc..Every kind of filler differs from one another.?
The materials such as grain active carbon, haydite, volcanic rock, polyurethane, rough surface, porosity height, large specific surface area, surface has affine
Property, it is easy to microorganism attachment and growth, forms biomembrane, and property is stablized, will not decomposes.Therefore, application is more.But poly- ammonia
Ester material quality is soft, bad mechanical property, is used for a long time and packing layer collapse phenomenon easily occurs；The materials such as haydite, volcanic rock contain greatly
The minerals of amount, by water, exhaust gas and the erosion of microorganism, surface forms rickle, blocks gas circuit, increases packing layer
The pressure loss, make reactor for treatment effect decline, energy consumption increase.
The inoculum for purifying the bioreactor of the exhaust gas containing organochlorine is mostly at organochlorine pesticide pollution soil or sewage
The activated sludge of factory is managed, the microorganism laundering period is long, and bioreactor starting is slow.Function bacterium after inoculation domestication enrichment, can contract
The starting time of short bioreactor.But in the microorganism of laboratory's domestication enrichment, competitiveness is weak, in practical applications,
Usually replaced by the strong microorganism of competitiveness.
Summary of the invention:
In order to solve, volatility organochlorine treatment effeciency in the prior art is low, microorganism is uneven in mixed carrier area load
It is even, starting is slow, the problem of filler collapses is used for a long time, the present invention provides the short shape bacillus of one plant of Nie Shi
(Brachybacterium nesterenkovii) NY-1, and the bacterial strain is applied in removing exhaust gas containing organochlorine.
The short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-1 according to the present invention, for from containing
The agricultural chemicals waste water domestication of the substances such as trichloroacetaldehyde, metrifonate, DDVP, phosphorus trichloride, isolated separation bacterium.The bacterial strain is
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on March 10th, 2017, deposit number is
CGMCC No.13747, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica,
Postcode 100101.
The bacterial strain feature is as follows:
Gram-positive bacteria, form are rod-short, and size is (0.5~0.75) μ m (1.0~2.5) μm, do not produce interior life
Spore, without motion；NY-1 bacterial strain can use Pfansteihl, acetic acid, pyruvic acid, α -one base-glutaric acid, D- galactolipin, rough gentian
Carbon source of the various saccharides substance such as disaccharides, alpha-D-glucose and D- xylose as the bacterial strain, also can use l-Alanine, L- paddy
The nitrogen source of propylhomoserin, altheine and butanediamine as it；NY-1 bacterial strain can produce catalase, Esterified Enzyme, alcohol dehydrogenase
A variety of enzymes such as enzyme and acetaldehyde dehydrogenase.
Growth temperature is 20-37 DEG C, and optimum growth temperature is 30 DEG C.Growth pH is 6-11, and the pH of optimum growth is 7.
Acid is produced using rhamnose, D- sucrose, D- melibiose etc., remains to survive in the case where pH is 11 strong basicity environment.
The present invention also provides a kind of microbial activity filler, the microbial activity filler is by the short shape bacillus of Nie Shi
(Brachybacterium nesterenkovii) NY-1 bacteria suspension is adsorbed on filler, and it is hollow that filler is seated in wire side
It is prepared in ball, it can better exhaust gas of the treatment and purification containing organochlorine.
The filler is one of polyurethane foamed blocks, haydite, volcanic rock or molecular sieve or a variety of；
The packing density is 20-900kg/m3, porosity 55-90%, partial size 4-85mm；
The mass ratio of the microbial bacteria suspension and filler is 2-90: 10；
The short shape bacillus NY-1 cell concentration of Nie Shi is 2.5 × 10 in the microbial activity filler7CFU/g -1.0×
1010CFU/g(filler)；
The wire side hollow sphere material is polyethylene, polyvinyl chloride or polypropylene；
The hollow bulb diameter 6cm of the wire side, is made of two hemisphere, and each hemisphere surface is porous, the aperture in each hole
2-8mm；
There is a circle reinforcing ring in the middle part of the wire side hollow sphere, reinforcing ring is equipped with lock, convenient for unlatching and closure two
Hemisphere.
Specific preparation process is as follows for the filler:
(1) preparation of the short shape bacillus NY-1 bacteria suspension of Nie Shi
1. the short shape bacillus NY-1 of Nie Shi is accessed into liquid fermentation medium after inclined-plane culture, by 10-25% inoculum concentration into
Row expands fermented and cultured step by step；
Fermentation culture conditions are as follows: in 36 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-
Under the conditions of 0.10Mpa, incubation time 48h；
The composition of the liquid fermentation medium are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO3
2.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeSO4·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilize 20min, is cooled to room temperature after sterilizing and adds 2.0mg/L trichloroacetaldehyde；
2. after fermented and cultured, fermentation liquid being placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, 10- is centrifuged
20min discards supernatant liquid, and precipitating is diluted with sterile nutrient solution, and acquisition cell concentration is the short shape bacillus of 0.5-1.5g/L Nie Shi
NY-1 bacteria suspension；
The composition of the sterile nutrient solution are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g
NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, 6.8-7.2,121 DEG C of pH value sterilizings
20min；
(2) preparation of microbial activity filler:
1. the filler of packet or grain shape is filled into wire side hollow sphere, wire side hollow sphere is with packing quality ratio
100:100-450；
2. being sprayed the short shape bacillus NY-1 bacteria suspension of Nie Shi of step (1) preparation by the hole of wire side hollow sphere to packet
Or the surface of the filler of grain shape；
It is mixed 3. the wire side hollow sphere that load has filler is put into rotate in impeller；
4. again to the short shape bacillus NY-1 bacteria suspension of wire side hollow sphere surface spraying Nie Shi that filler is housed；
It repeats step 1. -4. 2-3 time, equal appendix on filler is made in hollow ball surface and hole to have bacterium cell, acquisition Nie Shi
Short shape bacillus NY-1 cell concentration reaches 2.5 × 107CFU/g-1.0×1010CFU/g(filler)Microbial activity filler, 4 DEG C guarantor
It deposits spare.
It is specific to walk the present invention also provides a kind of method using mentioned microorganism active filler processing gas containing trichloroacetaldehyde
It is rapid as follows:
(1) above-mentioned appendix is had to micro- life of the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-1
Object active filler is placed in a container, is passed through the gas containing trichloroacetaldehyde, using trichloroacetaldehyde as carbon source and chlorine source, activates microorganism
Active filler；Activation time is 2-15 days；
The concentration of the trichloroacetaldehyde is 10-250mg/m3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas containing trichloroacetaldehyde, uses alunite
The short shape bacillus of family name (Brachybacterium nesterenkovii) NY-1 purifies the trichloroacetaldehyde in exhaust gas, microbial activity
Packing volume is 0.01-100m3, the total concentration of trichloroacetaldehyde is 10-250mg/m3, gas flow rate 0.05-10000m3/ h, stops
Time 0.5-5min is stayed, purification temperature is 20-35 DEG C；Periodically microorganism is maintained to microbial activity filler surface spraying nutrient solution
The activity of active filler, spray frequency are to spray 1 time for 1-3 days, and each spray rate is 1.0-200L/min, each spray time
For 10-90min；
The composition of the nutrient solution are as follows: glucose 0.5g, yeast powder 0.2g, KH2PO42.0g, NaHCO32.0g
KNO32.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, pH 6.5-7.0；
The degradation capability of tracing detection trichloroacetaldehyde, the present invention use gas chromatography tracing detection trichloroacetaldehyde；It is described
Gas chromatography are as follows: using Agilent GC-6890N chromatographic measure trichloroacetaldehyde concentration, chromatographic column HP-5
Capillary column (30m × 0.32mm × 0.25 μm), vaporizing chamber, FPD detector, column temperature are respectively set to 250 DEG C, 250 DEG C, 100
DEG C, sample volume 100 μ L, N2Flow velocity is 45mL/min.The results show that using above-mentioned filler to the discarded progress containing trichloroacetaldehyde
The degradation rate of purified treatment, trichloroacetaldehyde reaches 90% or more.
The utility model has the advantages that
1. the efficiency of the short shape bacillus NY-1 of Nie Shi of the invention degradation organochlorine with higher, up to 90-96%；And
The laundering period of dominant bacteria is short, can quickly start degradation reaction in 1 week, applies in exhaust gas bioreactor of the purification containing organochlorine
In, the starting period can be shortened.
2. the short shape bacillus NY-1 appendix of the Nie Shi that will degrade is in net using the effect of a variety of mechanics such as sedimentation, attachment and centrifugation
On the light porous material of face hollow ball inside, microbial activity filler is formed, bacterium cell can not only be uniformly adhered to fill out
Expect surface, and be able to enter in the hole inside filler, keeps the bacterium cell appendix amount of microbial activity filler high, be not easy from biology
It is lost in reactor.
3. Nie Shi is short, shape bacillus NY-1 competitiveness is very strong, can use chlorinated organics as carbon source and chlorine source, passes through
Grab Pfansteihl, acetic acid, pyruvic acid, α -one base-glutaric acid, D- galactolipin, gentiobiose, α-D- glucose and D- xylose etc.
Various saccharides substance generates a variety of enzymes such as catalase, Esterified Enzyme, alcohol dehydrogenase and acetaldehyde dehydrogenase.
It, being capable of the continuously and effectively purification volatilization in alkaline environment 4. the short shape bacillus NY-1 of Nie Shi has very strong alkali resistance
Property organic exhaust gas containing chlorine.
5. the rough surface, porous of the carrier material for microbial activity filler is suitable for bacterium cell and adheres to；Specific surface area
Greatly, be conducive to the volatility organochlorine such as bacterium cell and trichloroacetaldehyde to contact；The wire side hollow sphere hole for loading light filler is more, thoroughly
Gas is good, and resistance is small, and mass-transfer efficiency is high；Microbial activity filler weight is light, intensity is high, the pressure loss is small；High temperature resistant, corrosion resistant
Erosion；Not perishable deformation, can be used for a long time and adapt to the physical condition of Various Complex.
Detailed description of the invention:
The short shape bacillus of Fig. 1 Nie Shi (Brachybacterium nesterenkovii) NY-1 (NCBI GenBank sequences number
KY 979105) phylogenetic tree.
Specific embodiment:
In order to which the objects, technical solutions and advantages of this patent are more clearly understood, below in conjunction with specific embodiment, to this
Patent is further elaborated.It should be appreciated that specific embodiment described herein is only to explain this patent, and do not have to
It is of the invention in limiting.
Strain idenfication
It is extracted using Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and MO-BIO PowerSoil DNA
Kit (TaiWan, China dot nanometer technical concern Co., Ltd) extracts and the obtained short shape bacillus of bacterial strain Nie Shi of purifying
The DNA of (Brachybacterium nesterenkovii) NY-1.Take 5 μ L DNA samples under the conditions of 120V, 20-25min
Electrophoresis detection is carried out, observation quality inspection is carried out under gel-electrophoretic apparatus.
Select bacterial universal primers F16S-27 (5`-AGAGTTTGATCCTGGCTCAG-3`) and R16S-1492 (5`-
CGGTTACCTTGTTACGACTTC-3` PCR amplification) is carried out.PCR reaction condition are as follows: 94 DEG C of initial denaturation 3min, 94 DEG C of denaturation
30s, 54 DEG C of annealing 30s, 72 DEG C of extension 90s recycle 30 periods；Then 72 DEG C of extension 10min；Last 4 DEG C of holdings.
Successively by PCR product purifying and quality inspection, the connection of PCR purified product, E. coli competent it is heat-shock transformed,
And carry out plasmid extraction, sequencing.
Sequence merger using DNAMAN by the similitude measured greater than 97% is an OTU (operating unit), is chosen every
Sequence is represented in a OTU compare between sequence (1400bp or more length) using the GenBank database and BLAST of NCBI,
(Neighbour Joining) method phylogenetic tree construction (such as Fig. 1 is connected using Phylogenetic Analysis (MEGA3.1) software ortho position
It is shown).
A kind of microbial activity filler for removing the exhaust gas containing organochlorine of embodiment 1 and its preparation
A kind of microbial activity filler, the microbial activity filler are by the short shape bacillus of Nie Shi
(Brachybacterium nesterenkovii) NY-1 bacteria suspension is adsorbed on filler, and it is hollow that filler is seated in wire side
It is prepared in ball, it can better exhaust gas of the treatment and purification containing organochlorine.
The filler is volcanic rock；Packing density is 900kg/m3, porosity 55%, partial size 4mm；Microbial bacteria is outstanding
The mass ratio of liquid and filler is 2: 10；In microbial activity filler the short shape bacillus NY-1 cell concentration of Nie Shi be 2.5 ×
107CFU/g(filler)；
The wire side hollow sphere material is polyethylene；The hollow bulb diameter 6cm of wire side, is made of, Mei Geban two hemisphere
Ball surface is porous, the aperture 2mm in each hole；There is a circle reinforcing ring in the middle part of wire side hollow sphere, reinforcing ring is equipped with lock, convenient for opening
Open and be closed two hemisphere.
Specific preparation process is as follows for the filler:
(1) preparation of the short shape bacillus NY-1 bacteria suspension of Nie Shi
1. the short shape bacillus NY-1 of Nie Shi is accessed into liquid fermentation medium after inclined-plane culture, by 10% inoculum concentration carry out by
Grade expands fermented and cultured；
Fermentation culture conditions are as follows: in 36 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-
Under the conditions of 0.10Mpa, incubation time 48h；
The composition of the liquid fermentation medium are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO3
2.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeSO4·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilize 20min, is cooled to room temperature after sterilizing and adds 2.0mg/L trichloroacetaldehyde；
2. after fermented and cultured, fermentation liquid being placed in refrigerated centrifuge in 4 DEG C, 2000rpm, 10min is centrifuged, discards
Supernatant, precipitating are diluted with sterile nutrient solution, and acquisition cell concentration is the short shape bacillus NY-1 bacteria suspension of 0.5g/L Nie Shi；
The composition of the sterile nutrient solution are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g
NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, 6.8-7.2,121 DEG C of pH value sterilizings
20min；
(2) preparation of microbial activity filler:
1. the filler of square block is filled into wire side hollow sphere, wire side hollow sphere is 100:450 with packing quality ratio；
2. the short shape bacillus NY-1 bacteria suspension of Nie Shi of step (1) preparation is arrived filler by the hole spray of wire side hollow sphere
Surface；
It is mixed 3. the wire side hollow sphere that load has filler is put into rotate in impeller；
4. again to the short shape bacillus NY-1 bacteria suspension of wire side hollow sphere surface spraying Nie Shi that filler is housed；
1. -4. 3 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, acquisition Nie Shi is short
Shape bacillus NY-1 cell concentration reaches 2.5 × 107CFU/g(filler)Microbial activity filler, 4 DEG C save backup.
A kind of microbe stuffing for removing the exhaust gas containing organochlorine of embodiment 2 and its preparation
A kind of microbial activity filler, the microbial activity filler are by the short shape bacillus of Nie Shi
(Brachybacterium nesterenkovii) NY-1 bacteria suspension is adsorbed on filler, and it is hollow that filler is seated in wire side
It is prepared in ball, it can better exhaust gas of the treatment and purification containing organochlorine.
The filler is polyurethane foamed blocks；Packing density is 20kg/m3, porosity 90%, partial size 85mm；It is micro-
The mass ratio of biological bacteria suspension and filler is 90: 10；The short shape bacillus NY-1 cell concentration of Nie Shi is 1.0 in microbial activity filler
×1010CFU/g(filler)；
The wire side hollow sphere material is polypropylene；The hollow bulb diameter 6cm of wire side, is made of, Mei Geban two hemisphere
Ball surface is porous, the aperture 8mm in each hole；There is a circle reinforcing ring in the middle part of wire side hollow sphere, reinforcing ring is equipped with lock, convenient for opening
Open and be closed two hemisphere.
Specific preparation process is as follows for the filler:
(1) preparation of the short shape bacillus NY-1 bacteria suspension of Nie Shi
1. the short shape bacillus NY-1 of Nie Shi is accessed into liquid fermentation medium after inclined-plane culture, by 25% inoculum concentration carry out by
Grade expands fermented and cultured；
Fermentation culture conditions are as follows: in 36 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-
Under the conditions of 0.10Mpa, incubation time 48h；
The composition of the liquid fermentation medium are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO3
2.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeSO4·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilize 20min, is cooled to room temperature after sterilizing and adds 2.0mg/L trichloroacetaldehyde；
2. after fermented and cultured, fermentation liquid being placed in refrigerated centrifuge in 4 DEG C, 8000rpm, 20min is centrifuged, is abandoned
Supernatant is removed, precipitating is diluted with sterile nutrient solution, and acquisition cell concentration is the short shape bacillus NY-1 bacteria suspension of 1.5g/L Nie Shi；
The composition of the sterile nutrient solution are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g
NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, 6.8-7.2,121 DEG C of pH value sterilizings
20min；
(2) preparation of microbial activity filler:
1. granular filler is filled into wire side hollow sphere, wire side hollow sphere is 100:100 with packing quality ratio；
2. the short shape bacillus NY-1 bacteria suspension of Nie Shi of step (1) preparation is arrived filler by the hole spray of wire side hollow sphere
Surface；
It is mixed 3. the wire side hollow sphere that load has filler is put into rotate in impeller；
4. again to the short shape bacillus NY-1 bacteria suspension of wire side hollow sphere surface spraying Nie Shi that filler is housed；
1. -4. 2 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, acquisition Nie Shi is short
Shape bacillus NY-1 cell concentration reaches 1.0 × 1010CFU/g(filler)Microbial activity filler, 4 DEG C save backup.
A kind of microbe stuffing for removing the exhaust gas containing organochlorine of embodiment 3 and its preparation
A kind of microbial activity filler, the microbial activity filler are by the short shape bacillus of Nie Shi
(Brachybacterium nesterenkovii) NY-1 bacteria suspension is adsorbed on filler, and it is hollow that filler is seated in wire side
It is prepared in ball, it can better exhaust gas of the treatment and purification containing organochlorine.
The filler is molecular sieve；Packing density is 500kg/m3, porosity 75%, partial size 50mm；Microbial bacteria
The mass ratio of suspension and filler is 50: 10；In microbial activity filler the short shape bacillus NY-1 cell concentration of Nie Shi be 6.0 ×
108CFU/g(filler)；
The wire side hollow sphere material is polyvinyl chloride；The hollow bulb diameter 6cm of wire side, is made of, each two hemisphere
Hemisphere surface is porous, the aperture 5mm in each hole；There is a circle reinforcing ring in the middle part of wire side hollow sphere, reinforcing ring is equipped with lock, is convenient for
Open and be closed two hemisphere.
Specific preparation process is as follows for the filler:
(1) preparation of the short shape bacillus NY-1 bacteria suspension of Nie Shi
1. the short shape bacillus NY-1 of Nie Shi is accessed into liquid fermentation medium after inclined-plane culture, by 15% inoculum concentration carry out by
Grade expands fermented and cultured；
Fermentation culture conditions are as follows: in 36 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-
Under the conditions of 0.10Mpa, incubation time 48h；
The composition of the liquid fermentation medium are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO3
2.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeSO4·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilize 20min, is cooled to room temperature after sterilizing and adds 2.0mg/L trichloroacetaldehyde；
2. after fermented and cultured, fermentation liquid being placed in refrigerated centrifuge in 4 DEG C, 5000rpm, 15min is centrifuged, is abandoned
Supernatant is removed, precipitating is diluted with sterile nutrient solution, and acquisition cell concentration is the short shape bacillus NY-1 bacteria suspension of 1.0g/L Nie Shi；
The composition of the sterile nutrient solution are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g
NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, 6.8-7.2,121 DEG C of pH value sterilizings
20min；
(2) preparation of microbial activity filler:
1. the filler of square block is filled into wire side hollow sphere, wire side hollow sphere is 100:250 with packing quality ratio；
2. the short shape bacillus NY-1 bacteria suspension of Nie Shi of step (1) preparation is arrived filler by the hole spray of wire side hollow sphere
Surface；
It is mixed 3. the wire side hollow sphere that load has filler is put into rotate in impeller；
4. again to the short shape bacillus NY-1 bacteria suspension of wire side hollow sphere surface spraying Nie Shi that filler is housed；
1. -4. 3 time it repeats step, makes in hollow ball surface and hole that equal appendix has bacterium cell on filler, acquisition Nie Shi is short
Shape bacillus NY-1 cell concentration reaches 6.0 × 108CFU/g(filler)Microbial activity filler, 4 DEG C save backup.
A kind of method of the microbial activity filler of embodiment 4 processing exhaust gas containing organochlorine
The present invention also provides a kind of sides using 1 gained microbial activity filler of embodiment processing gas containing trichloroacetaldehyde
Method, the specific steps are as follows:
(1) 1 gained appendix of embodiment there is into the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-
1 microbial activity filler is placed in a container, is passed through the gas containing trichloroacetaldehyde, living using trichloroacetaldehyde as carbon source and chlorine source
Change microbial activity filler；Activation time is 15 days；
The concentration of the trichloroacetaldehyde is 20mg/m3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas containing trichloroacetaldehyde, uses alunite
Trichloroacetaldehyde in the short shape bacillus NY-1 purification exhaust gas of family name, microbial activity packing volume is 1m3, trichloroacetaldehyde in air inlet
Concentration be 20mg/m3, gas flow rate 100m3/ h, residence time 0.5min, purification temperature are 20 DEG C, pH 10-11；Periodically
The activity of microbial activity filler is maintained to microbial activity filler surface spraying nutrient solution, spray frequency is to spray 1 time for 1 day,
Each spray rate is 1.0L/min, and each spray time is 10min；Gas outlet trichloroacetaldehyde concentration is 1.0mg/m3, removal
Rate reaches 95%；
The composition of the nutrient solution are as follows: glucose 0.5g, yeast powder 0.2g, KH2PO42.0g, NaHCO32.0g
KNO32.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, pH 6.5-7.0.
A kind of method of the microbial activity filler of embodiment 5 processing exhaust gas containing organochlorine
The present invention also provides a kind of sides using 2 gained microbial activity filler of embodiment processing gas containing trichloroacetaldehyde
Method, the specific steps are as follows:
(1) 2 gained appendix of embodiment there is into the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-
1 microbial activity filler is placed in a container, is passed through the gas containing trichloroacetaldehyde, living using trichloroacetaldehyde as carbon source and chlorine source
Change microbial activity filler；Activation time is 2 days；
The concentration of the trichloroacetaldehyde is 250mg/m3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas containing trichloroacetaldehyde, uses alunite
Trichloroacetaldehyde in the short shape bacillus NY-1 purification exhaust gas of family name, microbial activity packing volume is 100 m3, three chloroethenes in air inlet
The concentration of aldehyde is 180mg/m3, gas flow rate 10000m3/ h, residence time 5min, purification temperature are 35 DEG C, pH 10-11；
The activity of microbial activity filler is periodically maintained to microbial activity filler surface spraying nutrient solution, spray frequency is 3 days sprays 1
Secondary, each spray rate is 200L/min, and each spray time is 90min；Gas outlet trichloroacetaldehyde concentration is 7.8mg/m3, go
Except rate reaches 95.7%；
The composition of the nutrient solution are as follows: glucose 0.5g, yeast powder 0.2g, KH2PO42.0g, NaHCO32.0g
KNO32.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, pH 6.5-7.0.
A kind of method of the microbial activity filler of embodiment 6 processing exhaust gas containing organochlorine
The present invention also provides a kind of sides using 3 gained microbial activity filler of embodiment processing gas containing trichloroacetaldehyde
Method, the specific steps are as follows:
(1) 3 gained appendix of embodiment there is into the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-
1 microbial activity filler is placed in a container, is passed through the gas containing trichloroacetaldehyde, living using trichloroacetaldehyde as carbon source and chlorine source
Change microbial activity filler；Activation time is 5 days；
The concentration of the trichloroacetaldehyde is 250mg/m3；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas containing trichloroacetaldehyde, uses alunite
Trichloroacetaldehyde in the short shape bacillus NY-1 purification exhaust gas of family name, microbial activity packing volume is 50 m3, trichloroacetaldehyde in air inlet
Concentration be 218mg/m3, gas flow rate 5000m3/ h, residence time 3min, purification temperature are 30 DEG C, pH 10-11；Periodically
The activity of microbial activity filler is maintained to microbial activity filler surface spraying nutrient solution, spray frequency is to spray 1 time for 2 days,
Each spray rate is 100L/min, and each spray time is 50min；Gas outlet trichloroacetaldehyde concentration is 10.3mg/m3, removal
Rate reaches 95.3%；
The composition of the nutrient solution are as follows: glucose 0.5g, yeast powder 0.2g, KH2PO42.0g, NaHCO32.0g
KNO32.0g, NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, pH 6.5-7.0.
Embodiment 7: Experimental comparison's effect
3 gained filler of embodiment is used for the processing of following gases:
(1) exhaust gas containing trichloroacetaldehyde, gas flow 0.2m are handled3/ h, residence time 8s, purification temperature 25
℃.Bioreactor continuous operation January, pH 10-11, the mean air entry concentration of trichloroacetaldehyde are as follows: 527mg/m3, gas concentration out
For 35mg/m3, removal rate reaches 91%.Cell concentration 6.0 × 108CFU/g(filler)。
Contrast and experiment is shown: when pH is 5-6, bioreactor continuous operation January, and the short shape bacillus NY-1 quantity of Nie Shi
From 6 × 108CFU/g increases to 9.2 × 108CFU/g.When pH in bioreactor is adjusted to 10-11, reactor is run 1 month
Afterwards, the short shape bacillus NY-1 quantity of Nie Shi is by 6 × 108CFU/g increases to 3.9 × 109CFU/g increases significant.After 3 months, Nie Shi
Short shape bacillus NY-1 quantity maintains 7.1 × 108-5.2×109CFU/g.As it can be seen that the short shape bacillus NY-1 of Nie Shi can be kept for a long time
Quantity and activity enable the trichloroacetaldehyde in the permanently effective removing exhaust gas of bioreactor.
(2) for handling the exhaust gas containing trichloroacetaldehyde of agricultural chemicals waste water treatment process generation, the concentration of trichloroacetaldehyde is 50mg/
m3, gas flow 5000m3/ h, residence time 85s, 25 DEG C of gas temperature, the removal rate of trichloroacetaldehyde is greater than 90%.
Equipment is run more than 2 months, and the short shape bacillus NY-1 quantity of pH 7-8.5, Nie Shi maintains 8.04 × 109-1.4×
1010CFU/g, reach purification trichloroacetaldehyde while, equipment can be run steadily in the long term.
In above-mentioned gas treatment process, the starting period of bioreactor is 3-7 days.And under equal conditions, it will be in filler
Strain replace with agricultural chemicals waste water processing activated sludge after, the starting period be 2 weeks-January.
Claims (9)
1. it is a kind of remove the exhaust gas containing organochlorine microbial activity filler, which is characterized in that the microbial activity filler be by
Nie Shi is short, and shape bacillus NY-1 bacteria suspension is adsorbed on filler, and filler is seated in wire side hollow sphere and is prepared；
The short shape bacillus of Nie Shi is specially the short shape bacillus of Nie Shi (Brachybacterium nesterenkovii) NY-1, is protected
Hiding number is CGMCC No.13747.
2. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described to fill out
Material is one of polyurethane foamed blocks, haydite, volcanic rock or molecular sieve or a variety of.
3. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described to fill out
Material density is 20-900kg/m3, porosity 55-90%, partial size 4-85mm.
4. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described
The mass ratio of microbial bacteria suspension and filler is 2-90: 10.
5. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that the net
The mass ratio of face hollow sphere and filler is 100:100-450.
6. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described
The short shape bacillus NY-1 cell concentration of Nie Shi is 2.5 × 10 in microbial activity filler7CFU/g-1.0×1010CFU/g(filler)。
7. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described
Wire side hollow sphere material is polyethylene, polyvinyl chloride or polypropylene.
8. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that described
The hollow bulb diameter 6cm of wire side, is made of two hemisphere, and each hemisphere surface is porous, the aperture 2-8mm in each hole；
There is a circle reinforcing ring in the middle part of the wire side hollow sphere, reinforcing ring is equipped with lock, convenient for two hemisphere of unlatching and closure.
9. a kind of microbial activity filler for removing the exhaust gas containing organochlorine as described in claim 1, which is characterized in that the alunite
The short shape bacillus NY-1 bacteria suspension of family name the preparation method is as follows:
1. the short shape bacillus NY-1 of Nie Shi is accessed into liquid fermentation medium after inclined-plane culture, by 10-25% inoculum concentration carry out by
Grade expands fermented and cultured；
Fermentation culture conditions are as follows: in 36 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-0.10Mpa item
Under part, incubation time 48h；
The composition of the liquid fermentation medium are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g
NH4Cl 0.5g, MgCl2·6H2O 0.5g, FeSO4·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizings
20min is cooled to room temperature after sterilizing and adds 2.0mg/L trichloroacetaldehyde；
2. after fermented and cultured, fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, 10-20min is centrifuged,
Liquid is discarded supernatant, precipitating is diluted with sterile nutrient solution, and it is outstanding for the short shape bacillus NY-1 bacterium of 0.5-1.5g/L Nie Shi to obtain cell concentration
Liquid；
The composition of the sterile nutrient solution are as follows: glucose 0.5g, KH2PO42.0g, NaHCO32.0g, KNO32.0g, NH4Cl
0.5g, MgCl2·6H2O 0.5g, FeCl2·7H2O 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min.
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