CN108624529B - A kind of application of the microbial inoculum comprising Meng Shi pseudomonad in purification chlorine contained exhaust gas - Google Patents
A kind of application of the microbial inoculum comprising Meng Shi pseudomonad in purification chlorine contained exhaust gas Download PDFInfo
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Abstract
The invention belongs to field of environmental improvement, and in particular to a kind of application in purification chlorine contained exhaust gas of the microbial inoculum containing Meng Shi pseudomonad.The Meng Shi pseudomonad is specially Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, deposit number is CGMCC No.13748, the microbial inoculum is used to handle the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, the concentration of trichloroacetaldehyde is 5-100mg/m3, trichloroacetaldehyde degradation efficiency can achieve 75% or more.
Description
Technical field:
The invention belongs to field of environmental improvement, and in particular to one kind includes Meng Shi pseudomonad (Pseudomonas
Monteilii) application of the microbial inoculum of LW-1 in purifying volatile organochlorine exhaust gas.
Background technique:
Waste water in pesticide producing contains a large amount of organosulfur pesticide and organo-chlorine pesticide intermediate, complicated component, concentration
High, strong toxicity.The product or in which mesosome of part organosulfur pesticide and organo-chlorine pesticide such as parathion, malathion, methyl pair
Sulphur phosphorus, trichloroacetaldehyde, phosphorus trichloride etc., have a volatility, and most of organic sulfur and organo-chlorine pesticide have it is pungent
Peculiar smell.
Parathion (C10H14NO5It PS) is colourless oil liquid, solubility is 24mg/L (25 DEG C) in water.It is slightly soluble in petroleum ether
And kerosene, there can be garlic odour with most immiscible organic solvents.It is promptly hydrolyzed in alkaline medium；In neutral or slightly acidic solution
In it is relatively stable；It is all unstable to ultraviolet light and air.There are mutagenicity and animal teratogenesis to microorganism.Belong to hypertoxic type, tool
There are acute toxicity, irritation, mutagenicity.Human lymphocyte Sister chromatid exohange is influenced when 200 μ g/L；10μmol/L
When influence human fibroblasts unscheduled DNA synthesis；When 10 μm of ol/L, rat lymphocyte DNA damage, IARC are made in 16 hours
Carcinogenicity is evaluated as G3 grades；The minimum toxic dose of rat oral (TDLo): 360 μ g/kg (pregnant 2~22d/ postpartum 15d) influence new
Raw mouse is biochemical and is metabolized；The minimum toxic dose of subcutaneous rat (TDLo): 9800 μ g/kg (pregnant 7~13d), lethal tire.Parathion energy
Enter human body by alimentary canal, respiratory tract and complete skin and mucous membrane.Parathion in environment can also be sent out by food chain
Raw biological concentration.
Malathion (C10H19O6PS2) it is colourless or pale yellow oily liquid, there is garlic smell.Chance open fire, high fever is flammable；With
Strong oxidizer reacts；Phosphorus, sulfur oxide gas are generated by thermal decomposition；It is toxic to animal, it is irritating to eyes, skin
Property；Active in 5.0 or less pH, pH7.0 or more is easy hydrolysis failure.Female mice acute oral LD50 is 1751.5mg/kg, male
Rat acute LD50 is 1634.5mg/kg, and the percutaneous LD50 of rat acute is 4000~6150mg/kg.Poisoning symptom is head
Bitterly, dizziness, nausea, inability, hidrosis, vomiting, salivation, eye-blurred, myosis, spasm, stupor, flesh fibrillation, pulmonary edema etc..
Generally morbidity is very fast after contact in a short time (oral, sucking, skin, mucous membrane) a large amount of organophosphorus pesticides.Malathion poisoning is dived
The volt phase is longer.It absorbs through skin, falls ill in 12h, poisoning symptom shows as appetite stimulator, Nausea and vomiting, abdominal pain, hidrosis, thing
Object is fuzzy, myosis, respiratory secretions increase, bronchial spasm expiratory dyspnea, pulmonary edema；Sample Symptoms are muscle bundle shake
It quivers, muscle spasmus, myoparalysis；Central nervous system symptom shows as headache, dizzy, insomnia drowsiness, out of strength, irritated, twitch living, dusk
Fan, brain edema.The state of an illness is heavier when oral ingestion, and gastrointestinal symptom becomes apparent from.Some cases can have cardiac muscle, liver, renal damage.
Parathion-methyl (C8H10NO5PS): colorless crystalline powder, industrial goods are brown or yellow liquid or solid, are met bright
Fire, high fever is flammable, by poisonous gas such as thermal decomposition release phosphorus, sulfur oxides, can decompose rapidly in lye.To big white mouse urgency
Property LD50 of passing through mouth be 14~24mg/kg, to rabbit acute percutaneous LD50 be 300~400mg/kg, belong to high toxic pesticide.Methyl is to sulphur
Phosphorus tool is tagged and stomach poison function, can inhibit the vigor of cholinesterase in pest nervous system and lethal, and insecticidal spectrum is wide, is often processed into
Missible oil or pulvis (see the formulations of pesticide) use, and are mainly used for prevention and treatment various agricultural pest.It is prominent with certain cause to microorganism
Denaturation, such as 667 μ g/ ware of salmonella typhimurium；Escherichia coli: 10mmol/L.Gaseous state, mist state, aerosol state or powdery
It is easily absorbed for respiratory tract, liquid can be through eye mucous membrane, skin or gastrointestinal tract.Into after in vivo, distribution to each device of whole body rapidly
Official's tissue, and be firmly combined with histone.
Organo-chlorine pesticide is one of current most popular insecticide, is occupied an important position in pesticide market.So
And in organo-chlorine pesticide production and agricultural chemicals waste water treatment process, there is volatile Pesticidal products or intermediate, waved from liquid phase
Hair, spills into air, forms air pollution.
Volatile pesticide and intermediate include: metrifonate, DDVP, phosphorus trichloride, dichlobenil, herbicide, thimet,
Phoxim, biphenyl, BFPO, alkane and alkane substitute class intermediate, aromatic intermediate etc. (choosing organochlorine), these substances
Most of have a pungent smell, some substances such as diethyl phosphite, trichloroacetaldehyde, belong to carcinogenicity, mutagenicity and
The cause property substance of the three of teratogenesis.
It is these organic sulfurs with peculiar smell, organic because being aerated, pushing the mechanical disturbances such as water flow in wastewater treatment process
Chlorine is spilt into air from water, forms odor pollution.If not being subject to processing appropriate, the organochlorine in air is spilt into,
Serious pollution environment, is detrimental to health.
Biotechnology cleans because it has many advantages, such as at low cost, easy to operate, technology, is without secondary pollution, is effective at present
Handle one of the important method containing organic compounds.The principle of biologic treating technique is to utilize the biological oxidation of microorganism will
Organic sulfur conversion is the process of the harmless or low harmful substances such as sulfate, carbon dioxide, water, as follows:
Malathion → carbon dioxide+sulfate+water；
Parathion-methyl → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water；
Parathion → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water.
The principle of biologic treating technique is to convert carbon dioxide, salt for organochlorine using the biological oxidation of microorganism
Hydrochlorate and water etc. be harmless or the process (formula 1) of low harmful substance.
C2HCl3O+O2→CO2+H2O+HCl (formula 1)
Existing research shows that the facultative chemoheterotrophy bacterium in natural environment belongs to pseudomonad (Pseudomonas
Sp.) X2 (201310551914.2), Sphingobacterium (Sphingobacterium griseoflavus) SCU-B140
(201510715812.9), Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans), thermophilic iron hook end
The mixed culture of spirillum (Leptospirillum ferriphilum) and Ferroplasma acidiphilum
(201310745138.X) etc. has degradation to organosulfur pesticide.But not yet discovery is for handling organic sulfur and organic
The Meng Shi pseudomonad (Pseudomonas monteilii) of chlorine, and use Meng Shi pseudomonad processing sulfur-bearing, contain chlorine pollution
The method of object.
Summary of the invention:
In order to solve, volatility organic sulfur, organochlorine treatment effeciency in the prior art be low, microorganism is on mixed carrier surface
Load is uneven, starting is slow, the problem of filler collapses is used for a long time, and the present invention provides one plant of Meng Shi pseudomonad
(Pseudomonas monteilii), and the bacterial strain is applied in removing exhaust gas containing organochlorine.
The Meng Shi pseudomonad, specially Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, be from
It is tamed and dociled in agricultural chemicals waste water containing the organochlorines such as the organic sulfurs such as parathion-methyl, malathion, parathion and phosphorus trichloride, trichloroacetaldehyde
Change, isolated separation bacterium.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms on March 10th, 2017
Common micro-organisms center, deposit number are CGMCC No.13748, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, Institute of Microorganism, Academia Sinica, postcode 100101.
The bacterial strain feature is as follows:
Meng Shi pseudomonad (Pseudomonas monteilii) LW-1: cell is in the shape of a rod, peritrichous, gram dye
Color is negative, and Starch Hydrolysis, VP, indole reaction are feminine gender, and gelatin liquefaction, glucose response are the positive.Bacterium colony is white, round,
Surface is smooth, moistens sticky, flat protrusion.In the natural environment, competitiveness is strong, wide adaptation range, can utilize carbon extensively
Hydrate, amino acid, organic acid be carbon source, pH wide adaptation range (pH 3.0-7.0), under high temperature (50 DEG C -60 DEG C) part all
It can grow, optimum growth temperature is 30-55 DEG C, pH 4.0-6.5.
The present invention also provides the microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1,
The microbial inoculum can be used for purifying the exhaust gas containing organochlorine, solve the pollution for the volatility organochlorine that the processes such as agricultural chemicals waste water processing generate
Problem, the microbial inoculum the preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in fermentation medium after inclined-plane culture, is expanded step by step by 10-25% inoculum concentration
Culture, fermentation condition: 35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-0.10Mpa condition
Under, incubation time 45-55h；
The fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min go out
It is cooled to room temperature after bacterium and adds 4.5mg/L trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid, which is placed in refrigerated centrifuge, discards supernatant liquid in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min,
Precipitating is diluted with sterile nutrient solution, obtains the bacteria suspension that cell concentration is 0.3-0.95g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility organochlorine exhaust gas
Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
The present invention also provides the above-mentioned microbial inoculums of use to handle the method containing organochlorine, specific as follows:
Aforesaid liquid microbial inoculum is supported on filler, for handle the generation of agricultural chemicals waste water reservoir containing the organic sulphur waste gas, gas
Body flow is 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, the concentration of trichloroacetaldehyde is 5-100mg/
m3, trichloroacetaldehyde degradation efficiency can achieve 75% or more；
The filler is one of polyurethane foamed blocks, haydite, volcanic rock or molecular sieve or a variety of, and Meng Shi is false in filler
Monad LW-1 cell concentration 1.0 × 105-9.0×108CFU/g(filler)。
The utility model has the advantages that
1. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 degradation organochlorine with higher of the invention
Efficiency, up to 75% or more；And the laundering period of dominant bacteria is short, and inoculation can quickly start degradation reaction in 1 week, applies
It purifies in the exhaust gas bioreactor containing organic sulfur, the starting period can be shortened.
2. using the effect of a variety of mechanics such as sedimentation, attachment and centrifugation, by the Meng Shi pseudomonad (Pseudomonas that degrades
Monteilii) LW-1 microbial inoculum appendix forms microbial activity filler, bacterium on the light porous material of wire side hollow ball inside
Cell can not only be uniformly adhered to filler surface, and be able to enter in the hole inside filler, make microbial activity filler
Bacterium cell appendix amount it is high, be not easy to be lost from bioreactor.
3. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 has, distribution is wide, quantity is more, nutritional need is simple
Feature single, breeding is fast, competition colonization power is strong.The pseudomonad inhibits other microorganisms, containing biting iron by secretion antibiotic
Element, the siderophore that can be secreted have a clear superiority in the competition of iron.
4. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 is chemoorganotrophy type, there is catalase,
It can utilize and decomposing volatile organic sulfur/organochlorine.
5. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 has antiviral activity, to TMV (Tobacco mosaic
Virus), the viruses such as PVY (marmor upsilon) have inhibiting effect, use is safer.
Detailed description of the invention:
Fig. 1 Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 phylogenetic tree.
Specific embodiment:
In order to which the objects, technical solutions and advantages of this patent are more clearly understood, below in conjunction with specific embodiment, to this
Patent is further elaborated.It should be appreciated that specific embodiment described herein is only to explain this patent, and do not have to
It is of the invention in limiting.
Strain idenfication:
DNA is extracted: being mentioned using Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and respective environment sample DNA
Take purification kit (Taiwan dot nanometer technical concern Co., Ltd) screen respectively acquisition bacterial strain DNA extract with it is pure
Change, bacterial universal primers F16S-27 and R16S-1492 is selected to carry out PCR amplification, primer sequence is respectively as follows:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction condition setting are as follows: 94 DEG C of initial denaturation 5min；Subsequent 94 DEG C of denaturation 30s, 56 DEG C of annealing 30s, 72 DEG C of extensions
90s recycles 25 periods；Then 72 DEG C of extension 10min；Last 4 DEG C of holdings 10min.By being purified to PCR product, after quality inspection
PCR purified product is connected on PMD-18T carrier, is then transformed into bacillus coli DH 5 alpha competent cell.After converting
50 μ L of competent cell be coated on the LB culture medium containing isopropylthiogalactoside, X-gal and ammonia benzyl.37 DEG C of constant temperature
Incubator is incubated overnight, and carries out LB liquid medium culture from each plate picking 25 white single bacterium spots.After culture 2 hours,
Culture solution is subjected to sequencing analysis.The sequence measured uses DNAMAN software to carry out similitude merger first, and similitude is greater than
97% sequence merger is an operating unit, and the representative sequence chosen in each operating unit utilizes BLAST and GenBank number
Homology analysis is carried out according to the 16SrDNA gene order in library, 1400bp or more length is chosen and is compared
(Clustelx1.83), Phylogenetic Analysis (MEGA3.1) is carried out using ortho position connection (Neighbour Joining) method, system
Development tree is as shown in Figure 1.It is compared by DNA sequence dna, which is Meng Shi pseudomonad (Pseudomonas monteilii),
It is named as Meng Shi pseudomonad (Pseudomonas monteilii) LW-1.
A kind of application of the microbial inoculum containing Meng Shi pseudomonad of embodiment 1 in purification exhaust gas containing organochlorine
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for
Exhaust gas containing organochlorine is purified, the organic phosphorus pollution problem of the volatility of the processes generation such as agricultural chemicals waste water processing, the microbial inoculum are solved
The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in fermentation medium after inclined-plane culture, expands training step by step by 10% inoculum concentration
It supports, fermentation condition: 35 DEG C, ventilation quantity 10L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, incubation time
55h；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings
20min；
The fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min go out
It is cooled to room temperature after bacterium and adds 4.5mg/L trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 2000rpm, centrifugal concentrating 20min, discards supernatant liquid, and precipitating uses nothing
Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.3g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility organochlorine exhaust gas
Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on polyurethane foamed blocks filler, Meng Shi pseudomonad LW-1 cell concentration in filler
5.0×106CFU/g(filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 100m3/ h, stops
Time 1.0min is stayed, 35 DEG C of temperature, the concentration of trichloroacetaldehyde is 42.6mg/m3, trichloroacetaldehyde degradation efficiency can achieve
80.3%
A kind of application of the microbial inoculum containing Meng Shi pseudomonad of embodiment 2 in purification exhaust gas containing organochlorine
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for
Exhaust gas containing organochlorine is purified, the pollution problem for the volatility organochlorine that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum are solved
The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in fermentation medium after inclined-plane culture, is amplified step by step by 25% inoculum concentration
Culture, fermentation condition: in 45 DEG C, ventilation quantity 100L/min, dissolved oxygen > 2.0mg/L under the conditions of tank presses 0.03-0.10Mpa, is trained
Support time 45h；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings
20min；
The fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min go out
It is cooled to room temperature after bacterium and adds 4.5mg/L trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 8000rpm, centrifugal concentrating 20min, discards supernatant liquid, and precipitating uses nothing
Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.8g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility organochlorine exhaust gas
Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on ceramics filling, Meng Shi pseudomonad LW-1 cell concentration 9.0 in filler ×
108CFU/g(filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 5000m3/ h, when stop
Between 5.0min, 55 DEG C of temperature, the concentration of trichloroacetaldehyde is 20.2mg/m3, the concentration of trichloroacetaldehyde is reduced to 3.6mg/ after processing
m3, degradation efficiency can achieve 82.2%.
A kind of application of the microbial inoculum containing Meng Shi pseudomonad of embodiment 3 in purification exhaust gas containing organochlorine
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for
Exhaust gas containing organochlorine is purified, the pollution problem for the volatility organochlorine that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum are solved
The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in fermentation medium after inclined-plane culture, expands training step by step by 18% inoculum concentration
It supports, fermentation condition: in 40 DEG C, ventilation quantity 50L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, when culture
Between 50h；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings
20min；
The fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min go out
It is cooled to room temperature after bacterium and adds 4.5mg/L trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, discards supernatant liquid, and precipitating uses nothing
Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.6g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility organochlorine exhaust gas
Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on volcanic filling material, Meng Shi pseudomonad LW-1 cell concentration 4.0 in filler ×
108CFU/g(filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 2500m3/ h, when stop
Between 3.0min, temperature 45 C, the concentration of trichloroacetaldehyde is 61.9mg/m3, trichloroacetaldehyde degradation efficiency can achieve 76.5%.
Claims (5)
1. a kind of application of microbial inoculum comprising Meng Shi pseudomonad in purification chlorine contained exhaust gas, which is characterized in that the Meng Shi is false
Monad is specially Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, and deposit number is CGMCC No.13748；
The microbial inoculum the preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 accesses fermentation medium after inclined-plane culture, carries out amplification step by step by 10-25% inoculum concentration and trains
It supports, fermentation condition: 35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa,
Incubation time 45-55h；
The fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl
0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min go out
It is cooled to room temperature after bacterium and adds 4.5mg/L trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid, which is placed in refrigerated centrifuge, discards supernatant liquid in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, precipitating
It is diluted with sterile nutrient solution, obtains the bacteria suspension that cell concentration is 0.3-0.95g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product；
The chlorine contained exhaust gas is trichloroacetaldehyde.
2. a kind of application of the microbial inoculum comprising Meng Shi pseudomonad as described in claim 1 in purification chlorine contained exhaust gas, special
Sign is that the sterile nutrient solution forms are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g,
NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings
20min。
3. a kind of application of the microbial inoculum comprising Meng Shi pseudomonad as described in claim 1 in purification chlorine contained exhaust gas, special
Sign is, specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on filler, for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas stream
Amount is 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, the concentration of trichloroacetaldehyde is 5-100mg/m3, three
Chloroacetaldehyde degradation efficiency can achieve 75% or more.
4. a kind of application of the microbial inoculum comprising Meng Shi pseudomonad as claimed in claim 3 in purification chlorine contained exhaust gas, special
Sign is that the filler is one of polyurethane foamed blocks, haydite, volcanic rock or molecular sieve or a variety of.
5. a kind of application of the microbial inoculum comprising Meng Shi pseudomonad as claimed in claim 3 in purification chlorine contained exhaust gas, special
Sign is, Meng Shi pseudomonad LW-1 cell concentration 1.0 × 10 in the filler5-9.0×108CFU/g(filler)。
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