CN108410779B - A kind of microbial inoculum comprising Meng Shi pseudomonad - Google Patents

A kind of microbial inoculum comprising Meng Shi pseudomonad Download PDF

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CN108410779B
CN108410779B CN201810456018.0A CN201810456018A CN108410779B CN 108410779 B CN108410779 B CN 108410779B CN 201810456018 A CN201810456018 A CN 201810456018A CN 108410779 B CN108410779 B CN 108410779B
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microbial inoculum
meng shi
liquid
parathion
shi pseudomonad
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李琳
薛松
王彦杰
杨凯雄
刘俊新
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Research Center for Eco Environmental Sciences of CAS
University of Chinese Academy of Sciences
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Abstract

The invention belongs to field of environmental improvement, and in particular to a kind of microbial inoculum containing Meng Shi pseudomonad and its application.The Meng Shi pseudomonad is specially Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, deposit number is CGMCC No.13748, the microbial inoculum be used to handle the generation of agricultural chemicals waste water reservoir containing the organic sulphur waste gas, gas flow 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, malathion, parathion, parathion-methyl total concentration be 20-200mg/m3, or the concentration of trichloroacetaldehyde is 5-100mg/m3, malathion, parathion, parathion-methyl degradation efficiency can achieve 90% or more, and trichloroacetaldehyde degradation efficiency can achieve 75% or more.

Description

A kind of microbial inoculum comprising Meng Shi pseudomonad
Technical field:
The invention belongs to field of environmental improvement, and in particular to one kind contains Meng Shi pseudomonad (Pseudomonas Monteilii) microbial inoculum of LW-1 and its application in purifying volatile organic sulfur/organochlorine exhaust gas.
Background technique:
Waste water in pesticide producing contains a large amount of organosulfur pesticide and organo-chlorine pesticide intermediate, complicated component, concentration High, strong toxicity.The product or in which mesosome of part organosulfur pesticide and organo-chlorine pesticide such as parathion, malathion, methyl pair Sulphur phosphorus, trichloroacetaldehyde, phosphorus trichloride etc., have a volatility, and most of organic sulfur and organo-chlorine pesticide have it is pungent Peculiar smell.
Parathion (C10H14NO5It PS) is colourless oil liquid, solubility is 24mg/L (25 DEG C) in water.It is slightly soluble in petroleum ether And kerosene, there can be garlic odour with most immiscible organic solvents.It is promptly hydrolyzed in alkaline medium；In neutral or slightly acidic solution In it is relatively stable；It is all unstable to ultraviolet light and air.There are mutagenicity and animal teratogenesis to microorganism.Belong to hypertoxic type, tool There are acute toxicity, irritation, mutagenicity.Human lymphocyte Sister chromatid exohange is influenced when 200 μ g/L；10μmol/L When influence human fibroblasts unscheduled DNA synthesis；When 10 μm of ol/L, rat lymphocyte DNA damage, IARC are made in 16 hours Carcinogenicity is evaluated as G3 grades；The minimum toxic dose of rat oral (TDLo): 360 μ g/kg (pregnant 2~22d/ postpartum 15d) influence new Raw mouse is biochemical and is metabolized；The minimum toxic dose of subcutaneous rat (TDLo): 9800 μ g/kg (pregnant 7~13d), lethal tire.Parathion energy Enter human body by alimentary canal, respiratory tract and complete skin and mucous membrane.Parathion in environment can also be sent out by food chain Raw biological concentration.
Malathion (C10H19O6PS2) it is colourless or pale yellow oily liquid, there is garlic smell.Chance open fire, high fever is flammable；With Strong oxidizer reacts；Phosphorus, sulfur oxide gas are generated by thermal decomposition；It is toxic to animal, it is irritating to eyes, skin Property；Active in 5.0 or less pH, pH7.0 or more is easy hydrolysis failure.Female mice acute oral LD50 is 1751.5mg/kg, male Rat acute LD50 is 1634.5mg/kg, and the percutaneous LD50 of rat acute is 4000~6150mg/kg.Poisoning symptom is head Bitterly, dizziness, nausea, inability, hidrosis, vomiting, salivation, eye-blurred, myosis, spasm, stupor, flesh fibrillation, pulmonary edema etc.. Generally morbidity is very fast after contact in a short time (oral, sucking, skin, mucous membrane) a large amount of organophosphorus pesticides.Malathion poisoning is dived The volt phase is longer.It absorbs through skin, falls ill in 12h, poisoning symptom shows as appetite stimulator, Nausea and vomiting, abdominal pain, hidrosis, thing Object is fuzzy, myosis, respiratory secretions increase, bronchial spasm expiratory dyspnea, pulmonary edema；Sample Symptoms are muscle bundle shake It quivers, muscle spasmus, myoparalysis；Central nervous system symptom shows as headache, dizzy, insomnia drowsiness, out of strength, irritated, twitch living, dusk Fan, brain edema.The state of an illness is heavier when oral ingestion, and gastrointestinal symptom becomes apparent from.Some cases can have cardiac muscle, liver, renal damage.
Parathion-methyl (C8H10NO5PS): colorless crystalline powder, industrial goods are brown or yellow liquid or solid, are met bright Fire, high fever is flammable, by poisonous gas such as thermal decomposition release phosphorus, sulfur oxides, can decompose rapidly in lye.To big white mouse urgency Property LD50 of passing through mouth be 14~24mg/kg, to rabbit acute percutaneous LD50 be 300~400mg/kg, belong to high toxic pesticide.Methyl is to sulphur Phosphorus tool is tagged and stomach poison function, can inhibit the vigor of cholinesterase in pest nervous system and lethal, and insecticidal spectrum is wide, is often processed into Missible oil or pulvis (see the formulations of pesticide) use, and are mainly used for prevention and treatment various agricultural pest.It is prominent with certain cause to microorganism Denaturation, such as 667 μ g/ ware of salmonella typhimurium；Escherichia coli: 10mmol/L.Gaseous state, mist state, aerosol state or powdery It is easily absorbed for respiratory tract, liquid can be through eye mucous membrane, skin or gastrointestinal tract.Into after in vivo, distribution to each device of whole body rapidly Official's tissue, and be firmly combined with histone.
Organo-chlorine pesticide is one of current most popular insecticide, is occupied an important position in pesticide market.So And in organo-chlorine pesticide production and agricultural chemicals waste water treatment process, there is volatile Pesticidal products or intermediate, waved from liquid phase Hair, spills into air, forms air pollution.
Volatile pesticide and intermediate include: metrifonate, DDVP, phosphorus trichloride, dichlobenil, herbicide, thimet, Phoxim, biphenyl, BFPO, alkane and alkane substitute class intermediate, aromatic intermediate etc. (choosing organochlorine), these substances Most of have a pungent smell, some substances such as diethyl phosphite, trichloroacetaldehyde, belong to carcinogenicity, mutagenicity and The cause property substance of the three of teratogenesis.
It is these organic sulfurs with peculiar smell, organic because being aerated, pushing the mechanical disturbances such as water flow in wastewater treatment process Chlorine is spilt into air from water, forms odor pollution.If not being subject to processing appropriate, the organochlorine in air is spilt into, Serious pollution environment, is detrimental to health.
Biotechnology cleans because it has many advantages, such as at low cost, easy to operate, technology, is without secondary pollution, is effective at present Handle one of the important method containing organic compounds.The principle of biologic treating technique is to utilize the biological oxidation of microorganism will Organic sulfur conversion is the process of the harmless or low harmful substances such as sulfate, carbon dioxide, water, as follows:
Malathion → carbon dioxide+sulfate+water；
Parathion-methyl → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water；
Parathion → p-nitrophenol+organic sulfate → sulfate+phosphate+carbon dioxide+water.
The principle of biologic treating technique is to convert carbon dioxide, salt for organochlorine using the biological oxidation of microorganism Hydrochlorate and water etc. be harmless or the process (formula 1) of low harmful substance.
C2HCl3O+O2→CO2+H2O+HCl (formula 1)
Existing research shows that the facultative chemoheterotrophy bacterium in natural environment belongs to pseudomonad (Pseudomonas Sp.) X2 (201310551914.2), Sphingobacterium (Sphingobacterium griseoflavus) SCU-B140 (201510715812.9), Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans), thermophilic iron hook end The mixed culture of spirillum (Leptospirillum ferriphilum) and Ferroplasma acidiphilum (201310745138.X) etc. has degradation to organosulfur pesticide.But not yet discovery is for handling organic sulfur and organic The Meng Shi pseudomonad (Pseudomonas monteilii) of chlorine, and use Meng Shi pseudomonad processing sulfur-bearing, contain chlorine pollution The method of object.
Summary of the invention:
In order to solve, volatility organic sulfur, organochlorine treatment effeciency in the prior art be low, microorganism is on mixed carrier surface Load is uneven, starting is slow, the problem of filler collapses is used for a long time, and the present invention provides one plant of Meng Shi pseudomonad (Pseudomonas monteilii), and the bacterial strain is applied to removing containing in organic sulfur and/or organochlorine exhaust gas.
The Meng Shi pseudomonad, specially Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, be from It is tamed and dociled in agricultural chemicals waste water containing the organochlorines such as the organic sulfurs such as parathion-methyl, malathion, parathion and phosphorus trichloride, trichloroacetaldehyde Change, isolated separation bacterium.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms on March 10th, 2017 Common micro-organisms center, deposit number are CGMCC No.13748, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Number, Institute of Microorganism, Academia Sinica, postcode 100101.
The bacterial strain feature is as follows:
Meng Shi pseudomonad (Pseudomonas monteilii) LW-1: cell is in the shape of a rod, peritrichous, gram dye Color is negative, and Starch Hydrolysis, VP, indole reaction are feminine gender, and gelatin liquefaction, glucose response are the positive.Bacterium colony is white, round, Surface is smooth, moistens sticky, flat protrusion.In the natural environment, competitiveness is strong, wide adaptation range, can utilize carbon extensively Hydrate, amino acid, organic acid be carbon source, pH wide adaptation range (pH 3.0-7.0), under high temperature (50 DEG C -60 DEG C) part all It can grow, optimum growth temperature is 30-55 DEG C, pH 4.0-6.5.
The present invention also provides the microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, The microbial inoculum can be used for purifying the exhaust gas containing organic sulfur and/or organochlorine, solve the volatility that the processes such as agricultural chemicals waste water processing generate The pollution problem of organic sulfur and/or organochlorine, the microbial inoculum the preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in liquid fermentation medium after inclined-plane culture, step by step by 10-25% inoculum concentration Amplification culture, fermentation condition: 35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, tank presses 0.03-0.10Mpa item Under part, incubation time 45-55h obtains fermentation liquid；
The liquid fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min is cooled to room temperature after sterilizing and adds 4.5mg/L parathion and/or trichloroacetaldehyde (when microbial inoculum is used to handle sulphur-containing exhaust gas 4.5mg/L parathion is added；4.5mg/L trichloroacetaldehyde is added when microbial inoculum is for handling chlorine contained exhaust gas；Microbial inoculum is for handling sulfur-bearing With addition 4.5mg/L parathion and 4.5mg/L trichloroacetaldehyde when chlorine contained exhaust gas)；
(2) liquid bacterial agent preparation method
Fermentation liquid, which is placed in refrigerated centrifuge, discards supernatant liquid in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, Precipitating is diluted with sterile nutrient solution, obtains the bacteria suspension that cell concentration is 0.3-0.95g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility organic sulfur, organic The processing of chlorine exhaust gas；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
The present invention also provides the above-mentioned microbial inoculums of use to handle the method containing organic sulfur and/or organochlorine exhaust gas, specific as follows:
Aforesaid liquid microbial inoculum is supported on filler, for handle the generation of agricultural chemicals waste water reservoir containing organic sulfur and/or having Machine chlorine exhaust gas, gas flow 100-5000m3/ h, residence time 1.0-5.0min, 35-55 DEG C of temperature, malathion, to sulphur Phosphorus, parathion-methyl total concentration be 20-200mg/m3, and/or the concentration of trichloroacetaldehyde is 5-100mg/m3, malathion, right Sulphur phosphorus, parathion-methyl degradation efficiency can achieve 90% or more, and trichloroacetaldehyde degradation efficiency can achieve 75% or more；
The filler is one of polyurethane foamed blocks, haydite, volcanic rock or molecular sieve or a variety of, and Meng Shi is false in filler Monad LW-1 cell concentration 1.0 × 105-9.0×108CFU/g(Filler)。
The utility model has the advantages that
1. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 degradation organic sulfur with higher of the invention With the efficiency of organochlorine, to the degradation efficiency of organic sulfur up to 90-100%, to the degradation efficiency of organochlorine up to 75% or more； And the laundering period of dominant bacteria is short, and inoculation can quickly start degradation reaction in 1 week, applies and is purifying the exhaust gas life containing organic sulfur In object reactor, the starting period can be shortened.
2. using the effect of a variety of mechanics such as sedimentation, attachment and centrifugation, by the Meng Shi pseudomonad (Pseudomonas that degrades Monteilii) LW-1 microbial inoculum appendix forms microbial activity filler, bacterium on the light porous material of wire side hollow ball inside Cell can not only be uniformly adhered to filler surface, and be able to enter in the hole inside filler, make microbial activity filler Bacterium cell appendix amount it is high, be not easy to be lost from bioreactor.
3. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 has, distribution is wide, quantity is more, nutritional need is simple Feature single, breeding is fast, competition colonization power is strong.The pseudomonad inhibits other microorganisms, containing biting iron by secretion antibiotic Element, the siderophore that can be secreted have a clear superiority in the competition of iron.
4. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 is chemoorganotrophy type, there is catalase, It can utilize and decomposing volatile organic sulfur/organochlorine.
5. Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 has antiviral activity, to TMV (Tobacco mosaic Virus), the viruses such as PVY (marmor upsilon) have inhibiting effect, use is safer.
Detailed description of the invention:
Fig. 1 Meng Shi pseudomonad (Pseudomonas monteilii) LW-1 phylogenetic tree.
Specific embodiment:
In order to which the objects, technical solutions and advantages of this patent are more clearly understood, below in conjunction with specific embodiment, to this Patent is further elaborated.It should be appreciated that specific embodiment described herein is only to explain this patent, and do not have to It is of the invention in limiting.
Strain idenfication:
DNA is extracted: being mentioned using Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and respective environment sample DNA Take purification kit (Taiwan dot nanometer technical concern Co., Ltd) screen respectively acquisition bacterial strain DNA extract with it is pure Change, bacterial universal primers F16S-27 and R16S-1492 is selected to carry out PCR amplification, primer sequence is respectively as follows:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction condition setting are as follows: 94 DEG C of initial denaturation 5min；Subsequent 94 DEG C of denaturation 30s, 56 DEG C of annealing 30s, 72 DEG C of extensions 90s recycles 25 periods；Then 72 DEG C of extension 10min；Last 4 DEG C of holdings 10min.By being purified to PCR product, after quality inspection PCR purified product is connected on PMD-18T carrier, is then transformed into bacillus coli DH 5 alpha competent cell.After converting 50 μ L of competent cell be coated on the LB culture medium containing isopropylthiogalactoside, X-gal and ammonia benzyl.37 DEG C of constant temperature Incubator is incubated overnight, and carries out LB liquid medium culture from each plate picking 25 white single bacterium spots.After culture 2 hours, Culture solution is subjected to sequencing analysis.The sequence measured uses DNAMAN software to carry out similitude merger first, and similitude is greater than 97% sequence merger is an operating unit, and the representative sequence chosen in each operating unit utilizes BLAST and GenBank number Homology analysis is carried out according to the 16SrDNA gene order in library, 1400bp or more length is chosen and is compared (Clustelx1.83), Phylogenetic Analysis (MEGA3.1) is carried out using ortho position connection (Neighbour Joining) method, system Development tree is as shown in Figure 1.It is compared by DNA sequence dna, which is Meng Shi pseudomonad (Pseudomonas monteilii), It is named as Meng Shi pseudomonad (Pseudomonas monteilii) LW-1.
A kind of microbial inoculum for purifying containing the organic sulphur waste gas of embodiment 1
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 accesses liquid fermentation medium after inclined-plane culture, amplifies training step by step by 10% inoculum concentration It supports, fermentation condition: 35 DEG C, ventilation quantity 10L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, incubation time 55h obtains fermentation liquid；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The liquid fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min is cooled to room temperature after sterilizing and adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 2000rpm, centrifugal concentrating 20min, discards supernatant liquid, and precipitating uses nothing Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.3g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, specific as follows:
Aforesaid liquid microbial inoculum is supported on polyurethane foamed blocks filler, Meng Shi pseudomonad LW-1 cell concentration in filler 1.0×105CFU/g(Filler), for handle the generation of agricultural chemicals waste water reservoir containing the organic sulphur waste gas, gas flow 100m3/ h, Residence time 1.0min, 35 DEG C of temperature, the concentration of malathion is 13.2mg/m3, parathion concentration be 33.7mg/m3, methyl The concentration of parathion is 8.3mg/m3, malathion removal rate is 92.6%, parathion degradation efficiency is 85.1%, methyl is to sulphur Phosphorus degradation efficiency can achieve 90.7%.
A kind of microbial inoculum for purifying containing the organic sulphur waste gas of embodiment 2
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in liquid fermentation medium after inclined-plane culture, is amplified step by step by 25% inoculum concentration Culture, fermentation condition: 45 DEG C, ventilation quantity 100L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, culture Time, 45h obtained fermentation liquid；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The liquid fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min is cooled to room temperature after sterilizing and adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 8000rpm, centrifugal concentrating 20min, discards supernatant liquid, and precipitating uses nothing Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.8g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, specific as follows:
Aforesaid liquid microbial inoculum is supported on ceramics filling, Meng Shi pseudomonad LW-1 cell concentration 9.0 in filler × 108CFU/g(Filler), for handle the generation of agricultural chemicals waste water reservoir containing the organic sulphur waste gas, gas flow 5000m3/ h is stopped Time 5.0min, 55 DEG C of temperature, malathion, parathion, parathion-methyl total concentration be 132.5mg/m3, malathion, Parathion, parathion-methyl degradation efficiency can achieve 91.4%.A kind of microbial inoculum for purifying containing the organic sulphur waste gas of embodiment 3
The microbial inoculum that one kind is prepared by Meng Shi pseudomonad (Pseudomonas monteilii) LW-1, the microbial inoculum can be used for Purification contains the organic sulphur waste gas, solves the pollution problem for the volatility organic sulfur that the processes such as agricultural chemicals waste water processing generate, the microbial inoculum The preparation method is as follows:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in liquid fermentation medium after inclined-plane culture, is amplified step by step by 18% inoculum concentration Culture, fermentation condition: 40 DEG C, ventilation quantity 50L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, when culture Between 50h obtain fermentation liquid；
Slant medium composition are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000ml, agar 20g, pH value 6.2-7.0,115 DEG C of sterilizings 20min；
The liquid fermentation medium are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgSO40.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min is cooled to room temperature after sterilizing and adds 4.5mg/L parathion；
(2) liquid bacterial agent preparation method
Fermentation liquid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, discards supernatant liquid, and precipitating uses nothing Bacterial nutrient solution dilution obtains the bacteria suspension that cell concentration is 0.6g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product, for volatility the organic sulphur waste gas Processing；
The sterile nutrient solution are as follows: glucose 0.5g, K2HPO40.5g, KH2PO41.5g, (NH4)2SO40.5g, NaCl 0.5g, MgCl20.2g, CaCl20.05g, FeSO40.02g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
Method containing the organic sulphur waste gas is handled using above-mentioned microbial inoculum, specific as follows:
Aforesaid liquid microbial inoculum is supported on volcanic filling material, Meng Shi pseudomonad LW-1 cell concentration 5.0 in filler × 107CFU/g(Filler), for handle the generation of agricultural chemicals waste water reservoir containing the organic sulphur waste gas, gas flow 2500m3/ h is stopped Time 3.0min, temperature 45 C, malathion, parathion, parathion-methyl concentration be respectively 16.3mg/m3, 20.8mg/m3 And 6.5mg/m3, malathion, parathion, parathion-methyl degradation efficiency can respectively reach 89.2%, 93.6% He 92.5%.
Embodiment 1 ' is a kind of for purifying the microbial inoculum of the exhaust gas containing organochlorine
Parathion in step (1) fermentation medium is only replaced with the trichlorine of equivalent with embodiment 1 by bacterial preparation process Acetaldehyde.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on polyurethane foamed blocks filler, Meng Shi pseudomonad LW-1 cell concentration in filler 5.0×106CFU/g(Filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 100m3/ h, Residence time 1.0min, 35 DEG C of temperature, the concentration of trichloroacetaldehyde is 42.6mg/m3, trichloroacetaldehyde degradation efficiency can achieve 80.3%.
Embodiment 2 ' is a kind of for purifying the microbial inoculum of the exhaust gas containing organochlorine
Parathion in step (1) fermentation medium is only replaced with three chloroethene of equivalent with embodiment 2 by bacterial preparation process Aldehyde.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on ceramics filling, Meng Shi pseudomonad LW-1 cell concentration 9.0 in filler × 108CFU/g(filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 5000m3/ h, when stop Between 5.0min, 55 DEG C of temperature, the concentration of trichloroacetaldehyde is 20.2mg/m3, the concentration of trichloroacetaldehyde is reduced to 3.6mg/ after processing m3, degradation efficiency can achieve 82.2%.
Embodiment 3 ' is a kind of for purifying the microbial inoculum of the exhaust gas containing organochlorine
Parathion in step (1) fermentation medium is only replaced with the trichlorine of equivalent with embodiment 3 by bacterial preparation process Acetaldehyde.
It is specific as follows using the method for above-mentioned microbial inoculum processing exhaust gas containing organochlorine:
Aforesaid liquid microbial inoculum is supported on volcanic filling material, Meng Shi pseudomonad LW-1 cell concentration 4.0 in filler × 108CFU/g(filler), for handling the exhaust gas containing organochlorine of agricultural chemicals waste water reservoir generation, gas flow 2500m3/ h, when stop Between 3.0min, temperature 45 C, the concentration of trichloroacetaldehyde is 61.9mg/m3, trichloroacetaldehyde degradation efficiency can achieve 76.5%.

Claims (2)

1. a kind of microbial inoculum comprising Meng Shi pseudomonad, which is characterized in that the Meng Shi pseudomonad is specially Meng Shi vacation unit cell Bacterium (Pseudomonas monteilii) LW-1, deposit number are CGMCC No.13748；The preparation method of the microbial inoculum is such as Under:
(1) zymotic fluid preparation method
Meng Shi pseudomonad LW-1 is accessed in liquid fermentation medium after inclined-plane culture, expands training step by step by 10-25% inoculum concentration It supports, fermentation condition: 35-45 DEG C, ventilation quantity 10-100L/min, dissolved oxygen > 2.0mg/L, under the conditions of tank presses 0.03-0.10Mpa, Incubation time 45-55h obtains fermentation liquid；
The liquid fermentation medium are as follows: glucose 0.5g, K2HPO40.5 g, KH2PO41.5 g, (NH4)2SO40.5 g, NaCl 0.5 g, MgSO40.2 g, CaCl20.05 g, FeSO40.02 g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizings 20min is cooled to room temperature after sterilizing and adds 4.5mg/L parathion or trichloroacetaldehyde；
(2) liquid bacterial agent preparation method
Fermentation liquid, which is placed in refrigerated centrifuge, discards supernatant liquid in 4 DEG C, 2000-8000 rpm, centrifugal concentrating 5-20min, precipitating It is diluted with sterile nutrient solution, obtains the bacteria suspension that cell concentration is 0.3-0.95g/L；
Bacteria suspension is packaged as liquid bacterial agent and forms Meng Shi pseudomonad microbial inoculum product.
2. a kind of microbial inoculum comprising Meng Shi pseudomonad as described in claim 1, which is characterized in that the sterile nutrient solution Are as follows: glucose 0.5g, K2HPO40.5 g, KH2PO41.5 g, (NH4)2SO40.5 g, NaCl 0.5 g, MgCl20.2 g, CaCl20.05 g, FeSO40.02 g, water 1000mL, pH 6.2-7.0,115 DEG C of sterilizing 20min.
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CN103146665A (en) * 2013-02-04 2013-06-12 中国科学院沈阳应用生态研究所 Organophosphorus pesticide degrading enzyme transformed by random mutation and encoding gene thereof
CN103923893A (en) * 2014-04-24 2014-07-16 华东理工大学 Organic phospholipid hydrolase, gene, recombinant expression transformant and application thereof
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