CN108294032A - Bactericidal composition containing Carbatene - Google Patents
Bactericidal composition containing Carbatene Download PDFInfo
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- CN108294032A CN108294032A CN201810107287.6A CN201810107287A CN108294032A CN 108294032 A CN108294032 A CN 108294032A CN 201810107287 A CN201810107287 A CN 201810107287A CN 108294032 A CN108294032 A CN 108294032A
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- carbatene
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
- A01N43/42—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings condensed with carbocyclic rings
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- Agronomy & Crop Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention provides a kind of Synergistic biocidal compositions containing Carbatene, it is characterized by comprising active components As and B, wherein, A is Carbatene, B is one or more kinds of combinations in copper 8-hydroxyquinolinate, sad mantoquita, further includes the auxiliary agent that can be used in Pesticide formulation, but do not include basic amino acid analog assistant, conventional pesticidal preparations are made, and are applied to controlling plant diseases.
Description
Technical field
The invention belongs to pesticides application technical fields, are related to a kind of composition pesticide, specifically killing containing Carbatene
Bacteria composition is applied to controlling plant diseases.
Background technology
In agricultural production process, plant disease occurs seriously, to endanger the nutrition organs such as axis, leaf and fruit, kind
The organs of multiplication such as son, stem tuber position, influences photosynthesis of plant, influences Leaf-feeding insects appearance, crop yield is made to fall sharply, under quality
Drop is serious.When disease occurs serious, crop is caused to be had no harvest.Therefore, plant disease in produce reality there is important prevention to anticipate
Justice, fungicide are the methods the most economic, quick and effective of controlling plant diseases.
Single bactericide use easy tos produce drug resistance or prevention spectrum is limited, not can be well solved in produce reality
Plant disease endangers.Mixture fungicide delays the advantages such as phytopathogen drug resistance with that can expand prevention spectrum, enhancing preventive effect,
Become most popular application form in production practice.Fungicide especially with synergy, each effective group of fungicide
It interacts between point, makes combination aging fruit better than the theoretical summation of exclusive use effect, thus thing can be played in actual use
The effect of half work(times, is more favored by user.
Carbatene (metiram) is the fungicide developed by overseas enterprise, belongs to thiol reaction agent, is mainly used for anti-Yellow River harnessing
Shot hole, rust in the various crops such as melon, tomato, capsicum, Chinese cabbage, potato, apple, grape, citrus, cotton, peanut, beet
The multiple diseases such as disease, black spot, leaf spot, downy mildew, late blight.CAS accession number is 9006-42-2.Its structural formula is as follows：
Copper 8-hydroxyquinolinate (oxine copper), sad mantoquita (copper octanoate) are the fungicide of two kinds of broad spectrum activities,
Excellent preventive effect is shown to the plant disease in various crop.
The present invention passes through a large amount of formulation screening test the study found that when without using basic amino acid analog assistant, Dai Sen
Join and arbitrarily combined with one or more kinds of medicaments in copper 8-hydroxyquinolinate, sad mantoquita, that is, shows surprising bactericidal effect,
Good synergistic effect is shown, practical bactericidal effect is substantially better than the theoretical summation of exclusive use, has in production non-
Often it is widely applied foreground.
Invention content
The present invention relates to Synergistic biocidal composition, including active components A and B, the active components A is Carbatene
(metiram), the active constituent B is one or more kinds of combinations in copper 8-hydroxyquinolinate, sad mantoquita.In addition, further including
The auxiliary agent that can be used in Pesticide formulation considers that the auxiliary agent does not include spy based on use cost and economic and environment-friendly angle
Different auxiliary agent basic amino acid analog assistant.
The composition can be used to prevent the plant disease in various useful crops with particularly good effect, described useful
Crop include but not limited to conventional breeding crop, genetically modified crops, domesticating and cultivating wild plant etc., especially suitable for prevent vegetable
Multiple diseases on the crops such as dish, fruit tree, ornamental plant, potato, cotton, peanut, beet, tobacco, soybean, corn, wheat.
The specific example that can be mentioned that is the representative plant disease that composition for preventing and controlling of the invention can be used in some, the example
It is not limited to specified genus kind.It should be noted that the invention is not limited in examples in detail below, can be extended with same way
To other plant diseases, bracket indicates its cause of disease Latin name：
Cucumber downy mildew (Pseudoperonospora cubensis), downy mildew of garpe (Plasmopara
Viticola), Chinese cabbage downy mildew (Peronospora parasitica), potato and tomato late blight (Phytophthora
Infestans), Vegetables in Brassica clubroot (Plasmodiophora brassicae), tobacco black shank (Phytophthora
Parasitica var.nicotianae), capsicum epidemic disease (Phytophthora capsic), ornamental plant corruption mildew
(Pythium splendens), lawn rot index (Pythium aphanidermatum), wild cabbage samping off (Pythium
Aphanidermatum), scab of apple (Venturia inaequalis), powdery mildew (Podosphaera
Leucotricha), spot defoliation (Alternaria mali), rot disease (Valsa mali), ring spot
(Physalospora piricola), brown spot (Marssonina coronaria), Cabbage Leaf Spot (Alternaria
Brassicae), tomato, capsicum and target (Alternaria solani), cucumber anthracnose
(Colletotrichum orbiculare), citrus and pepper anthracnose (Colletotrichum glecosporioides),
Strawberry, grape and apple anthracnose (Glomerella cingulata), banana anthracnose (Colletotrichum musae),
Sigatoka (Mycosphaerella fijiensis), cucurbitaceae vegetable (cucumber, cucurbita pepo, pumpkin) powdery mildew
(Sphaerotheca fuliginea), powdery mildew of strawberry (Sphaerotheca aphanis), grapevine anthracnose
(Sphaceloma ampelinum), powdery mildew (Erysiphe necator), white rot (Coniothyrium
Diptodictta), citrus scab (Sphaceloma fawcettii), yellow twig (Liberobacter asiaticum),
Penicilliosis (Penicillium italicum), peach brown rot (Monilinia fructicola), sweet cherry brown rot
(Monilinia laxa), mango blossom-end rot (Phomopsis mangiferae), peanut sclerotium rolfsii (Sclerotium
Rolfsii), leaf spot (Cercospora arachidicola), crown rot (Aspergillus niger), cotton verticillium wilt
(Verticillium dahliae), soybean rust (Phakopsora pachyrhizi), purple spot (Cercospora
Kikuchii), watermelon grafting (Mycosphaerella melonis), Alternaria alternate (Alternaria
Alternata), black scurf of potato and rice sheath blight disease (Rhizoctonia solani), potato silver maize ear rot
(Helminthosporium solani), ornamental flower black spot (Septoria fhrysanth), diseases and insect pests of edible mushroom is (such as brown
Maize ear rot Mycogone perniciosa, brown spot Verticillium fungicola, green mold Trichoderma viride,
Neurospora Neurospora crassa etc.), peach gummosis (Botryosphaeria ribis), Leaf Spot Caused by Corynespora cassiicola on Cucumber
(Corynespora cassiicola), beet cercospora leaf spot (Cercospora beticola), the leaf blight of corn
(Exserohilum turcicum), base rot disease (Fusarium graminearum), helminthosporium maydis (Bipolaris
Maydis), wheat avenge mould leaf blight (Monographella nivalis), septoria musiva leaf blight (Septoria tritici),
Powdery mildew (Blumeria graminis), leaf rust (Puccinia recondita), stripe rust (P.striiformis), bar
Rust (P.graminis), flax leaf spot (Cochliobolus miyabeanus), beans, alfalfa, bulb class, oil
Expect crops samping off and the damping-offs (Rhizoctonia solani) such as crop, sorghum, beet, sunflower, corn, cotton, west
Melon and cotton wilt, root rot (Fusarium oxysporum), peanut root rot (Fusarium solani), fruit
The crops such as tree, vegetables gray mold (Botrytis cinerea), rape, sclerotium blight of sunflower (Sclerotinia
Sclerotiorum), rape balck shank (Phoma lingam), citrus bacterial canker disease (Xanthomonas campestris), cigarette
Careless wildfire (Pseudomonas syringae pv.tabaci), tobacco, tomato and eggplant bacterial wilt (Pseudomonas
Solanacearum), cucumber bacterial angular leaf spot (Pseudomonas syringae pv.lachrymans), the soft corruption of Chinese cabbage
Sick (Erwinia carotovora) etc..
In order to solve the above-mentioned technical problem, the present invention is realised by adopting the following technical scheme：
The present composition component A and B can be with arbitrary proportion mixtures, and usually a kind of active component content is higher than rest activity
Ingredient, preferably mixing ratio are 100：1 to 1：100 or 50：1 to 1：50.
The present composition can be by mixing active constituent with the auxiliary agent that can be used in Pesticide formulation, with known
Method is prepared as conventional preparation, such as missible oil, suspending agent, water dispersible granules, aqueous emulsion, microemulsion, wettable powder, particle
Agent, dispersible oil-suspending agent, microcapsule suspending agent, suspension emulsion etc..The active constituent of 0.1~95% weight is typically contained in preparation,
It is preferred that the active constituent of 0.5~90% weight.
The auxiliary agent that can be used in Pesticide formulation includes but not limited to：Water, solvent, filler, various surfactants
(emulsifier, dispersant, wetting agent etc.), adhesive, colorant, antifreezing agent, thickener, suspending agent, disintegrant, disappear at film forming agent
Infusion, bleeding agent, warning coloration, stabilizer, wall capsule material, pH adjusting agent, preservative, synergist etc..Also, suitably, in order to
It improves to specific crop tolerance, safener can be properly added；It, can be in mixture group or sometimes for crop growth is promoted
It closes and adds conventional agriculture fertilizer in object, medicament-fertilizer mixture is made.These auxiliary agents be all commonly use in pesticidal preparations or allow to use at
Point, it is not particularly limited, one or more auxiliary agent may be selected and constitute, specific ingredient and dosage pass through letter according to recipe requirements
Single experiment determines.Auxiliary agent in the present composition does not include basic amino acid analog assistant (such as polylysine and its derivative
Deng).
The production technology of the various application forms of composition belongs to existing known technology, and details are not described herein.
The present composition can use in a variety of ways, be such as watered spraying in a usual manner and use, or directly spread fertilizer over the fields or ditch
It applies, or mixes pesticide-clay mixture and spread fertilizer over the fields, vegetative growth phase and reproductive stage can be used before plant emergence, after emergence.Dosage can
Change in wider range, and depending on situation, application method, crop, plant disease type to be prevented and the seedling of soil
Age size, weather conditions at that time and other factors.The present composition usually with 0.001~2.2kg mixture of active principles/
The dosage application of hectare, or applied with the dosage of 1~2200mg mixture of active principles/kg；It is further preferable that with 0.01~
The dosage of 1.0kg mixture of active principles/hectare is applied, and is applied with the dosage of 10~1000mg mixture of active principles/kg.
Compared with prior art, the present invention having the following technical effect that：
1, there is mixture composition good synergistic effect, application effect to be substantially better than single dose theoretical effect summation, that is, have
There is super superposition, can preferably control plant disease occurrence injury；
2, there is good complementarity in control of plant disease spectrum between mixture composition active constituent, can expands very well
Prevention spectrum, synthesis effectively control various plant disease occurrence injuries；
3, mixture composition has good synergistic effect, can reduce pharmaceutical use, reduces use cost and environment is dirty
Dye improves the safety to crop.
Specific implementation mode
Specific embodiments of the present invention are given below, it should be noted that the invention is not limited in implement in detail below
Example, all equivalent transformations made on the basis of the technical solutions of the present application each fall within protection scope of the present invention.
Embodiment 1：57% missible oil
Formula composition is：Active components A is 38%, and active constituent B is 19%, emulsifier triphenyl vinyl phenol polyoxyethylene
Ether 3%, emulsifier aerosol-OT salt 4%, solvent xylene complements to 100%.
Preparation method is：All materials are put into batching kettle, stirring and dissolving is to fully transparent, after chemical examination is qualified, transfer
It is filling to storage tank.
Embodiment 2：45% suspending agent
Formula composition is：Active components A is 5%, and active constituent B is 40%, wetting agent octadecyl sodium sulfosuccinate
5%, dispersant naphthalenesulfonic acid-formaldehyde condensate 4%, antifreeze glycol 5%, thickener xanthans 0.3%, water complements to
100%.
Preparation method is：By in active constituent and wetting agent, dispersant, antifreezing agent and water input stirred tank, it is sufficiently stirred
Afterwards, material is pumped into sand mill and is fully ground, after the completion of grinding, is pumped into high-speed shearing machine, after thickener is added, into
Row high speed shear obtains suspending agent after the completion of shearing.
Embodiment 3：81% water dispersible granules
Formula composition is：Active components A is 72%, and active constituent B is 9%, wetting agent calcium dodecyl benzene sulfonate 4%,
Dispersant alkyl phenol polyoxyethylene ether phosphate 6%, disintegrant ammonium sulfate 4%, adhesive polyethylene glycol 0.5%, filler are concave-convex
Stick soil complements to 100%.
Preparation method is：After mixing by all materials, it crushes through airslide disintegrating mill, is uniformly mixed again, then, add
Enter a certain amount of water to mediate this mixture, extruder grain, through dry screening to get to water dispersible granules.
Embodiment 4：27% aqueous emulsion
Formula composition is：Active components A is 9%, and active constituent B is 18%, solvent xylene 10%, solvent cyclohexanone
8%, emulsifier polyoxyethylene sorbitan fatty acid ketone 6%, emulsifier fatty alcohol polyoxyethylene ether 3%, antifreezing agent the third two
Alcohol 5%, water complements to 100%.
Preparation method is：After active components A and B are fully dissolved with solvent, input emulsifier forms oil after being sufficiently stirred
Phase；Antifreezing agent is added to the water dissolving, forms water phase；Water phase is slowly added in oil phase, is sheared using high-speed shearing machine, you can
Obtain aqueous emulsion.
Embodiment 5：15% microemulsion
Formula composition is：Active components A is 12%, and active constituent B is 3%, solvent xylene 10%, solvent Solvesso
2004%, emulsifier styrylphenol polyoxyethylene ether 7%, emulsifier sorbitan ester 8%, antifreezing agent glycerine 4%,
Water complements to 100%.
Preparation method is：After active components A and B are fully dissolved with solvent, puts into emulsifier and antifreezing agent is uniformly mixed,
It is eventually adding deionized water, carries out high speed shear, you can obtains microemulsion.
Embodiment 6：77% wettable powder
Formula composition is：Active components A is 11%, and active constituent B is 66%, wetting agent lauryl sodium sulfate 4%, point
Powder alkyl phenol polyoxyethylene ether phosphate 7%, filler kaolin complements to 100%.
Preparation method is：Active constituent and each auxiliary agent are uniformly mixed, puts into mechanical disintegrating machine and just smash, later
It is crushed through airslide disintegrating mill, remixes uniformly, obtain wettable powder.
Embodiment 7：8% granule
Formula composition is：Active components A is 4%, and active constituent B is 4%, wetting agent dioctyl sodium sulphosuccinate
0.9%, three phenethyl phenol polyoxyethylene ether phosphate 0.8% of dispersant, colorant carbon black 0.2%, filler bentonite complements to
100%.
Preparation method is：After mixing by all materials, a certain amount of water is added to mediate this mixture, extruding is made
Grain, through dry screening to get to granule.
Embodiment 8：35% dispersible oil-suspending agent
Formula composition is：Active components A is 30%, and active constituent B is 5%, emulsifier castor oil polyoxyethylene ether 8%,
Emulsifier disecoctylmaleate sodium sulfonate 5%, dispersant sodium lignin sulfonate 2%, thickener white carbon 1.5%, point
Dispersion media corn oil complements to 100%.
Preparation method is：By in active constituent and emulsifier, dispersant and decentralized medium input stirred tank, it is sufficiently stirred
Afterwards, it is pumped into high-speed shearing machine, carries out high speed shear, then pump and be fully sanded into sand mill, be added and increase after sand milling is qualified
It thick dose, stirs evenly up to dispersible oil-suspending agent.
Embodiment 9：20% microcapsule suspending agent
Formula composition is：Active components A is 4%, and active constituent B is 16%, solvent xylene 10%, solvent O-phthalic
Diethyl phthalate 5%, oiliness capsule skin Toluene-2,4-diisocyanate, 4- diisocyanate 4%, aqueous capsule skin diethylenetriamine 3%, emulsifier alkyl phenol
Polyoxyethylene ether formaldehyde condensation products 7%, dispersant polycarboxylate 5%, thickener aluminium-magnesium silicate 0.2%, decentralized medium water is supplied
To 100%.
Preparation method is：After active components A and B are fully dissolved with solvent, oiliness capsule skin is added, is sufficiently stirred to form oil
Phase；It is poured into the water phase for being mixed with emulsifier and dispersant, carries out high speed shear, aqueous capsule skin is then added, stirs evenly,
It then raises temperature to 70 DEG C of heat preservation solidifications 2 hours, microcapsules is made, are eventually adding thickener, stir evenly up to microcapsule suspending agent.
Embodiment 10：64% suspension emulsion
Formula composition is：Active components A is 48%, and active constituent B is 16%, solvent xylene 10%, solvent
Solvesso150 6%, emulsifier styrylphenol formaldehyde resin Polyoxyethylene polyoxypropylene ether 8%, wetting agent fatty alcohol
Ethoxylate 4%, dispersant alkyl naphthalene formaldehyde condensation product sodium sulfonate 3%, antifreezing agent propylene glycol 5%, thickener xanthans
0.3%, water complements to 100%.
Preparation method is：Active components A, wetting agent, dispersant, antifreezing agent and water are put into stirred tank, are sufficiently stirred
Afterwards, material is pumped into sand mill and is fully ground, suspending agent is made；After active constituent B is fully dissolved with solvent with breast
Agent is uniformly mixed, and is then added to it in Manufactured suspending agent under the conditions of high speed shear, and thickener is added, and is carried out high
Speed shearing, suspension emulsion is obtained after the completion of shearing.
Application effect example：
1, test method
(1) to Elsinǒe ampelina, botrytis cinerea, phytophthora capsici, sugar beet leaf spot bacteria, the late blight of potato
Bacterium, apple anthrax bacteria and Alternaria brassicae bioactivity
Reference《NYT 1156.2-2006 farm-chemical indoor determination test rule fungicide part 2s：Inhibit cause of disease true
Bacterium mycelia growth test Plating》It carries out.
With Elsinǒe ampelina (Sphaceloma ampelinum), botrytis cinerea (Botrytis cinerea),
Phytophthora capsici (Phytophthora capsic), sugar beet leaf spot bacteria (Cercospora beticola), potato late blight
Germ (Phytophthora infestans), apple anthrax bacteria (Glomerella cingulata), Alternaria brassicae
(Alternaria brassicae) is for trying pathogen.
Incidence tissue is acquired from field, laboratory isolates and purifies, is seeded on culture medium and cultivates, and culture to colony diameter reaches
5cm~6cm or so is spare.Phytophthora infestans oat medium culture, it is Elsinǒe ampelina, botrytis cinerea, peppery
The PDA culture medium culture of green pepper epidemic disease bacterium, sugar beet leaf spot bacteria and Alternaria brassicae, apple anthrax bacteria are trained with MEA culture mediums
It supports.
Oat medium formula：Oat 50g, agar powder 18g, deionized water 1000mL.
PDA culture medium is laboratory conventional medium, omits its formula herein.
MEA culture medium prescriptions：Malt soaks juice 20g, agar 15g, deionized water 1000mL.
It is spare after the sterilizings such as culture medium, deionized water, scribing line triangular flask, culture dish, pipette tips, card punch, inoculator.
Under aseptic technique, advance thawing is cooled to about 50 DEG C of sterilising medium and is quantitatively adding sterilizing scribing line three
In the bottle of angle, keep culture medium concordant with (60mL) at scribing line, then draw 600 μ L liquids successively from low concentration to high concentration, respectively
It is added in above-mentioned triangular flask, fully shakes up, rear equivalent is poured into 4 culture dishes (Φ 9cm), and it is flat that 5 concentration gradient band medicines are made
Plate.And it sets the processing without medicament and makees blank control.It will cultured pathogen, aseptically use sterilizing on culture medium
Card punch (Φ 5mm) cuts bacteria cake from the same circumference of colony edge, with inoculator by pure culture biscuits involvng inoculation in drug containing tablet center, bacterium
Silk down, cover ware lid, in incubator dark culturing (phytophthora infestans in 20 DEG C culture, apple anthrax bacteria and
Alternaria brassicae is cultivated in 28 DEG C of cultures, remaining germ in 25 DEG C).According to mycelia growing state in blank control ware, with card
Ruler measures colony diameter.Each bacterium colony uses crossing method vertical measurement, takes its average value, calculates the inhibition grown to mycelia
Rate.
The inhibiting rate that each concentration for the treatment of grows mycelia is calculated by formula (1), (2), unit is percentage (%), calculates knot
Fruit retains 2 significant digits.
D=D1-D2………………………………………(1)
In formula：
D-bacterium colony increases diameter, and unit is millimeter (mm)；
D1- colony diameter, unit are millimeter (mm)；
D2- bacteria cake diameter, unit are millimeter (mm).
In formula：
I-mycelial growth inhibition rate, unit are percentage (%)；
D0- blank control bacterium colony increases diameter, and unit is millimeter (mm)；
Dt- chemicals treatment bacterium colony increases diameter, and unit is millimeter (mm).
(2) to the bioactivity of bacterium of downy mildew of cucumber
Reference《The 7th part of NYT 1156.7-2006 farm-chemical indoor determination test rules fungicide：Prevent cucumber cream
Mildew tests basin and cuts out method》It carries out.
It is for trying germ with bacterium of downy mildew of cucumber (Pseudoperonospora cubensis), laboratory is seeded in cucumber
Expand on seedling numerous spare.
It is for studying object, laboratory potting with cucumber susceptible variety " Chang Chun Mi Ci ", seedling grows to 4~6 leaf periods
It is spare.
Prepared liquid is uniformly sprayed on " Chang Chun Mi Ci " cucumber seedling leaf two sides to whole wettings, after chemicals treatment
It is inoculated with for 24 hours.It chooses and expands numerous incidence of leaf, wash inferior lobe piece back side Pseudoperonospora cubensis sporangiums with 4 DEG C of distillations, eluent is double-deck
Filtered through gauze is made a concentration of 1 × 105The sporangia suspension of a sporangium/mL, by its spray inoculation in vacuum side of blade (frost
Mildew bacterium easily infects vacuum side of blade).Then under the conditions of 17~22 DEG C, 12h alternation of light and darkness, relative humidity be 90% or more item
It is cultivated under part.4 repetitions of often processing setting, often repeatedly 5 basin, per 2 plants of basin, and sets the processing without medicament and makees blank control.When
When blank control incidence reaches 80% or more, it is classified incidence of investigating.Calculate disease index and the prevention of each processing
Effect.
Disease index is calculated by formula (3), and control effect is calculated by formula (4), and result of calculation retains 2 significant digits.
In formula：
X-disease index；
Ni- sick the number of sheets at different levels；
I-is with respect to value of series；
N-investigation total number of sheets；
9-highest values of series.
In formula：
P-control effect, unit are percentage (%)；
CK-blank control disease index；
PT-chemicals treatment disease index.
(3) to the bioactivity of citrus processing
Reference《The 16th part of NY/T 1156.16-2008 farm-chemical indoor determination test rules fungicide：Inhibit bacterium
Increment tests turbidity method》It carries out.
It is to divide from the incidence tissue of field for trying germ with citrus processing (Xanthomonas campestris)
From, purifying it is spare.
NA culture medium prescriptions：Polyprotein peptone 5g, yeast powder 1g, beef extract 3g, sucrose 15g, agar 17g, deionized water
1000mL is adjusted to pH7.0 with the NaOH of 10mol/L.
NB Liquid Culture based formulas：Peptone containing polyprotein 5g, yeast powder 1g, beef extract 3g, sucrose 15g, deionized water
1000mL is adjusted to pH7.0 with the NaOH of 10mol/L.
Prepared liquid medicine quantitative is added in sterilizing NB culture mediums after cooling, is then put down production in NA culture mediums
Strain on plate is diluted to 1 × 10 with aqua sterilisa7The bacteria suspension of a spore/mL concentration connects respectively into each processing NB culture mediums
Kind 100uL bacterium solutions, shaken cultivation under the conditions of being placed in 28~30 DEG C, rotating speed 120r/min.4 repetitions are often handled, and sets and is free of
Blank control is made in the processing of medicament.The turbidity of each processing is measured respectively before starting culture, waits for that control treatment reaches logarithmic growth
When the phase, the turbidity of each processing is measured and recorded.
According to investigation result, the growth inhibition ratio of bacterium is calculated by formula (5), unit is percentage (%), result of calculation
Retain 2 significant digits.
In formula：
R-growth inhibition ratio；
A0- blank control turbidity value added；
A1- chemicals treatment turbidity value added.
(4) compositions contemplated activity judges
Compositions contemplated activity, which is used for reference in the transnational agricultural chemicals enterprise formulation patents such as Bayer, Syngenta, Tao Shi, judges composition
The method (COLBY methods) of expection activity：COLBY, S.R.：“Calculating Synergistic and Antagonistic
Responses of Herbicide Combinations ", Weeds 15, p.20~22,1967 carry out.
Two-spot compositions contemplated activity is calculated by (6) formula：
In formula：
Preventive effect when 1 dosage of X-medicament is P or inhibiting rate；
Preventive effect when 2 dosage of Y-medicament is Q or inhibiting rate；
Practical preventive effect after E-medicament 1 is mixed according to the above ratio with medicament 2 or inhibiting rate, the i.e. reality of mixture composition
Activity；
E0The theoretical preventive effect or inhibiting rate that 1 dosage of-medicament is 2 dosage of P+ medicaments when being Q, the i.e. expection of mixture composition
Activity.
As E ＞ E0When, i.e., if the substantial activity of Mixed Pharmacy is more than calculated expection activity, the composition just has
There is super superposition, that is, acts synergistically.
Ternary composition expection activity is calculated by (7) formula：
In formula：
Preventive effect when 1 dosage of X-medicament is P or inhibiting rate；
Preventive effect when 2 dosage of Y-medicament is Q or inhibiting rate；
Preventive effect when 3 dosage of Z-medicament is R or inhibiting rate；
E0The theoretical preventive effect or inhibiting rate that 1 dosage of-medicament is 2 dosage of P+ medicaments when be 3 dosage of Q+ medicaments being R, that is, combine
The expection activity of object；
Practical preventive effect when E-medicament 1,2 and 3 is by different ratio mixture or inhibiting rate, the i.e. substantial activity of composition；When
E ＞ E0When, i.e., if the substantial activity of Mixed Pharmacy is more than calculated expection activity, just there is the composition super superposition to make
With acting synergistically.
2, test result
Test result refers to table 1~9, it is seen then that active components A (Carbatene) be selected from active constituent B (copper 8-hydroxyquinolinate and octanoic acid
Mantoquita) medicament mixture after, substantial activity is all higher than expection activity, illustrates that the medicament mixture in Carbatene and active constituent B has
There is synergistic effect.
1 Carbatene of table is with copper 8-hydroxyquinolinate mixture to the synergy effect of Elsinǒe ampelina
2 Carbatene of table is with sad mantoquita mixture to the synergy effect of botrytis cinerea
3 Carbatene of table is with sad mantoquita mixture to the synergy effect of phytophthora capsici
4 Carbatene of table is with copper 8-hydroxyquinolinate mixture to the synergy effect of sugar beet leaf spot bacteria
5 Carbatene of table is with copper 8-hydroxyquinolinate mixture to the synergy effect of bacterium of downy mildew of cucumber
The synergy effect of 6 Carbatene of table and sad mantoquita mixture Phytophthora infestans
7 Carbatene of table, sad mantoquita are with copper 8-hydroxyquinolinate mixture to the synergy effect of citrus processing
8 Carbatene of table, sad mantoquita are with copper 8-hydroxyquinolinate mixture to the synergy effect of apple anthrax bacteria
9 Carbatene of table, copper 8-hydroxyquinolinate are with sad mantoquita mixture to the synergy effect of Alternaria brassicae
Claims (5)
1. bactericidal composition, which is characterized in that including active components A and B.
2. bactericidal composition as described in claim 1, which is characterized in that the active components A is Carbatene.
3. bactericidal composition as described in claim 1, which is characterized in that the active constituent B is copper 8-hydroxyquinolinate, sad mantoquita
In one or more kinds of combinations.
4. bactericidal composition as claimed in claim 1,2 or 3, which is characterized in that further include agriculture other than active components A and B
The auxiliary agent that can be used in medicine preparation processing, but do not include basic amino acid analog assistant.
5. the bactericidal composition as described in claim 1,2,3 or 4, which is characterized in that be applied to controlling plant diseases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810107287.6A CN108294032A (en) | 2018-02-02 | 2018-02-02 | Bactericidal composition containing Carbatene |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810107287.6A CN108294032A (en) | 2018-02-02 | 2018-02-02 | Bactericidal composition containing Carbatene |
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| CN201810107287.6A Withdrawn CN108294032A (en) | 2018-02-02 | 2018-02-02 | Bactericidal composition containing Carbatene |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101171921A (en) * | 2007-10-15 | 2008-05-07 | 张少武 | Bactericide agent composition containing active ingredient of metiram |
| CN101810181A (en) * | 2010-04-15 | 2010-08-25 | 浙江海正化工股份有限公司 | Sterilizing pesticide combination, preparation method and application thereof |
| CN105660659A (en) * | 2016-01-11 | 2016-06-15 | 江苏辉丰农化股份有限公司 | Bactericide composition |
| CN107827736A (en) * | 2017-12-08 | 2018-03-23 | 成都蓉橙九天生物科技有限公司 | A kind of preparation method of cupric octoate and sad copper products |
-
2018
- 2018-02-02 CN CN201810107287.6A patent/CN108294032A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101171921A (en) * | 2007-10-15 | 2008-05-07 | 张少武 | Bactericide agent composition containing active ingredient of metiram |
| CN101810181A (en) * | 2010-04-15 | 2010-08-25 | 浙江海正化工股份有限公司 | Sterilizing pesticide combination, preparation method and application thereof |
| CN105660659A (en) * | 2016-01-11 | 2016-06-15 | 江苏辉丰农化股份有限公司 | Bactericide composition |
| CN107827736A (en) * | 2017-12-08 | 2018-03-23 | 成都蓉橙九天生物科技有限公司 | A kind of preparation method of cupric octoate and sad copper products |
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