CN108277153A - A kind of culture systems and application method of the haematococcus pluvialis being suitable for the north - Google Patents
A kind of culture systems and application method of the haematococcus pluvialis being suitable for the north Download PDFInfo
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- CN108277153A CN108277153A CN201810402613.6A CN201810402613A CN108277153A CN 108277153 A CN108277153 A CN 108277153A CN 201810402613 A CN201810402613 A CN 201810402613A CN 108277153 A CN108277153 A CN 108277153A
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- 241000168517 Haematococcus lacustris Species 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims description 15
- 241000195493 Cryptophyta Species 0.000 claims abstract description 118
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 84
- 238000012258 culturing Methods 0.000 claims abstract description 71
- 238000011534 incubation Methods 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000005286 illumination Methods 0.000 claims description 28
- 229910001220 stainless steel Inorganic materials 0.000 claims description 10
- 239000010935 stainless steel Substances 0.000 claims description 10
- 230000029553 photosynthesis Effects 0.000 claims description 8
- 238000010672 photosynthesis Methods 0.000 claims description 8
- 238000009413 insulation Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000008676 import Effects 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 238000012549 training Methods 0.000 claims description 5
- 229910000611 Zinc aluminium Inorganic materials 0.000 claims description 4
- HXFVOUUOTHJFPX-UHFFFAOYSA-N alumane;zinc Chemical compound [AlH3].[Zn] HXFVOUUOTHJFPX-UHFFFAOYSA-N 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 210000004899 c-terminal region Anatomy 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 3
- 239000003973 paint Substances 0.000 claims description 3
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- 239000010902 straw Substances 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 2
- 238000005524 ceramic coating Methods 0.000 claims 1
- 230000012010 growth Effects 0.000 description 13
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 7
- 235000013793 astaxanthin Nutrition 0.000 description 7
- 239000001168 astaxanthin Substances 0.000 description 7
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 7
- 229940022405 astaxanthin Drugs 0.000 description 7
- 230000005484 gravity Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 241000168525 Haematococcus Species 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000195627 Chlamydomonadales Species 0.000 description 1
- 241000196319 Chlorophyceae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- 229910004616 Na2MoO4.2H2 O Inorganic materials 0.000 description 1
- -1 Tetrahydrate manganese chloride Chemical class 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- QCGGXGCODUUTLZ-UHFFFAOYSA-N [Na].[Na].[Na].[Na] Chemical compound [Na].[Na].[Na].[Na] QCGGXGCODUUTLZ-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000000490 cosmetic additive Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- NQXWGWZJXJUMQB-UHFFFAOYSA-K iron trichloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].Cl[Fe+]Cl NQXWGWZJXJUMQB-UHFFFAOYSA-K 0.000 description 1
- 150000004715 keto acids Chemical class 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 229940099607 manganese chloride Drugs 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- AQRDGTBNWBTFKJ-UHFFFAOYSA-N molybdenum;dihydrate Chemical compound O.O.[Mo] AQRDGTBNWBTFKJ-UHFFFAOYSA-N 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- FDEIWTXVNPKYDL-UHFFFAOYSA-N sodium molybdate dihydrate Chemical compound O.O.[Na+].[Na+].[O-][Mo]([O-])(=O)=O FDEIWTXVNPKYDL-UHFFFAOYSA-N 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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Abstract
The invention discloses a kind of culture systems for the haematococcus pluvialis being suitable for the north, including underground microdisk electrode tank, ground solar energy water-storing device and ground micro algae culturing device, the double layers of tank body structure that the underground microdisk electrode tank is made of inner and outer tank, the inner cavity of the inner canister being internally provided with for cultivating haematococcus pluvialis is equipped with cycle incubation cavity between the outer tank and inner canister；It is connected respectively with return duct I by water inlet pipe I between the ground solar energy water-storing device and cycle incubation cavity, the water inlet pipe I is equipped with the water pump I that flow is pumped into ground solar energy water-storing device from cycle incubation cavity；The inner cavity of the inner canister is connected by water inlet pipe II and return duct II respectively with ground micro algae culturing device, and the water inlet pipe II is equipped with the water pump II that haematococcus pluvialis is pumped into ground micro algae culturing device from inner cavity.By using this system, haematococcus pluvialis culture-liquid temp can be enable to be maintained at 20 DEG C~28 DEG C throughout the year, and northern substantial light is made full use of to shine.
Description
Technical field
The invention belongs to haematococcus pluvialis culture technique fields, and in particular to a kind of haematococcus pluvialis suitable for the north
Culture systems and application method.
Background technology
Haematococcus pluvialis belongs to Chlorophyta, Chlorophyceae, volvocales, haematococcus section, haematococcus.The dry powder of haematococcus pluvialis
Middle content astaxanthin can reach 5%, be the optimal raw material of generally acknowledged extraction astaxanthin.It is enriched in, nitrogen and phosphorous nutrient strong compared with dim light
Under conditions of, haematococcus pluvialis exists with green travelling vegetative cell state, and astaxanthin accumulation amount is less under the state, and cell is
Green；And under stress conditions (bloom photograph, with high salt, nitrogen hunger), haematococcus pluvialis exists in the form of motionless akinete, simultaneously
Largely accumulation astaxanthin, cell become red in the cell.
Astaxanthin (Astaxanthin, 3-3 '-dihydroxy-β-β ' carrotene -4-4 '-diketone) is a kind of keto-acid class Hu
Radish element, molecular formula C40H52O4.Astaxanthin is anti-oxidant, can remove free radical, anti-oxidation function is higher than carrotene, corn
Huang Zhi, bilirubin etc., oxidation resistance are more than 500 times of vitamin E.With anticancer effect.It can be used as in commercial Application
Cosmetic additive agent；Also feed addictive is can be used as, using having effect outstanding especially in salmon breeding process, can be made
The meats such as salmon are scarlet, bright in colour.
Because the vegetative cell of haematococcus pluvialis grows preference temperature at 24 DEG C or so, meet the region of growth with Yunnan
It is represented to be prominent.Yunnan has inborn climatic superiority, and the temperature difference is more northern much smaller throughout the year, proper temperature growth.
The northern area of China, has a basic condition of haematococcus pluvialis cultivation, especially sufficient illumination condition, but northern area four
Season is clearly demarcated, and winter temperature is too low, and summer temperature is excessively high, not high suitable for the temperature range of haematococcus pluvialis growing.The present invention is through grinding
Study carefully discovery, carries out large-scale industrialization culture in northern area, it is the control of algae cultivation temperature to need the critical issue solved.
Invention content
To overcome the deficiencies of existing technologies, the purpose of the present invention is by providing a kind of haematococcus pluvialis being suitable for the north
Culture systems and application method, haematococcus pluvialis in north culture thermophilic time short skill larger to solve northern annual range of temperature
Art problem.
To achieve the above object, the technical scheme is that a kind of training system for the haematococcus pluvialis being suitable for the north
System includes ground solar energy water-storing device and ground micro algae culturing device more than ground and the ground to locate below ground level
Lower microdisk electrode tank, the double layers of tank body structure that the underground microdisk electrode tank is made of inner and outer tank, the inner canister it is interior
Portion is equipped with the inner cavity for cultivating haematococcus pluvialis, and cycle incubation cavity is equipped between the outer tank and inner canister；The ground sun
It can be connected respectively with return duct I by water inlet pipe I between water-storing device and cycle incubation cavity, the water inlet pipe I is equipped with will
Flow is pumped into the water pump I of ground solar energy water-storing device from cycle incubation cavity；The inner cavity of the inner canister is filled with ground microdisk electrode
It sets and is connected respectively with return duct II by water inlet pipe II, the water inlet pipe II is equipped with is pumped into ground by haematococcus pluvialis from inner cavity
The water pump II of upper micro algae culturing device.
Preferably, the top of the inner canister is equipped with head cover, the water inlet pipe II and return duct II can be passed through from head cover to
It is connected to the inner cavity and ground micro algae culturing device；The top of the head cover is equipped with the dirt bed area of coverage and tubular service area, institute
It states tubular service area and is used for away pipeline and tubular service, the top cover in the tubular service area is equipped with sealing thermal insulation plate, described
Sealing thermal insulation plate includes stainless steel plate and is fixed on the plastic-spraying adiabator layer of stainless steel surface.
Preferably, the ground solar energy water-storing device includes temperature-controlled tube, thermal-collecting tube and the storage being connected with thermal-collecting tube
Hydroecium is connected by water inlet pipe I, return duct I respectively between the water storage room and cycle incubation cavity.
Preferably, the ground micro algae culturing device includes several micro algae culturing device main bodys and in communication micro-
Algae culturing liquid circulating line；The micro algae culturing device main body uses colume type transparent polybag.
Further, the micro algae culturing liquid circulating line include multiple laterals and main pipeline I arranged in parallel and
Main pipeline II, the main pipeline I are located at the top of main pipeline II；The micro algae culturing device main body is 4 and vertical placement, often
Top and bottom end are connected by a lateral with main pipeline I, main pipeline II respectively in a micro algae culturing device main body；The master
Pipeline I is being equipped with the ports A close to one end of water inlet pipe II, and the other end is equipped with the ends D for adding algae, culture medium and water
Mouthful；The main pipeline II is being equipped with the ports B close to one end of return duct II, and the other end is equipped with the C-terminal mouth for algae solution recycling.
It is preferred that the bottom of the inner canister is located at 5m-8m from the ground, the bottom of the outer tank is located at 6m-9m from the ground；
The thickness of the dirt bed area of coverage is 3-5m.
Preferably, the return duct I is equipped with control valve I；The return duct II is equipped with control valve II；The water inlet pipe
The bottom of I is close to cycle incubation cavity bottom, the bottom of the bottom of the water inlet pipe II close to inner cavity.
Preferably, it is equipped with temperature detector in the cycle incubation cavity and/or inner cavity of inner canister；In the cycle incubation cavity
Temperature detector be tightly attached on the outer wall of inner canister.
Preferably, the interior intracavitary of the inner canister is additionally provided with charger；The charger is inverted T font, the inflation
Equipment includes E fonts branched pipe, vertically disposed inflation vertical tube and horizontally disposed inflation transverse tube, the inflation transverse tube installation
In the bottom of inflation vertical tube, the E fonts branched pipe is two groups, is separately mounted to the left and right ends of inflation transverse tube, the E fonts
Branched pipe is equipped with multiple stomatas for finally discharging the air-flow for flowing through inflation vertical tube, inflation transverse tube.
Preferably, 0~0.5VVM of gas pushing quantity of the charger is 0~3kg/cm to keep the pressure of inner cavity2。
Further, the main body of the underground microdisk electrode tank uses stainless steel material；The outer wall of the outer tank, inner wall and
The outer wall surface of the inner canister is all made of spray zinc-aluminium technique, then spray paint；The inner wall surface of the inner canister is equipped with ceramics and applies
Layer.
Further, the present invention also provides the users of the culture systems of the haematococcus pluvialis for being suitable for the north
Method comprising following steps：
(1) environment temperature more than ground is at 20-28 DEG C, the haematococcus pluvialis only interior progress of algae culturing device on the ground
Culture；
(2) when the environment temperature more than ground is 28 DEG C high, it is micro- that the micro algae culturing liquid containing haematococcus pluvialis imports underground
It is cultivated in algae culture tank, and is pumped into the micro algae culturing device of ground by water pump II cycles and carries out photosynthesis；
(3) when environment temperature is less than 20 DEG C, the micro algae culturing liquid containing haematococcus pluvialis imports underground microdisk electrode tank
In cultivated, by ground solar energy water-storing device and cycle incubation cavity cycle between water circulation, control the interior of inner canister
The temperature of intracavitary heats the micro algae culturing liquid in inner cavity to 20-28 DEG C, and periodically by the microalgae containing haematococcus pluvialis
Culture solution is pumped into the micro algae culturing device of ground by water pump II cycles and carries out photosynthesis.
Preferably, culture light intensity is controlled by way of being covered with straw screen or mat to ground micro algae culturing device,
Intensity of illumination is set to be maintained at 20~50 μm of ol m-2s-1。
Preferably, the intensity of illumination is maintained at 30 μm of ol m-2s-1。
Compared with prior art, the method for the present invention has the following advantages that：
In the present invention, underground microdisk electrode tank is mainly kept the temperature using the inertia of soil, whichever, can in season
The opposite maintenance level for keeping the temperature at 10~25 DEG C adjusts culture tank culture-liquid temp, no in conjunction with solar energy water-storing device
Only the temperature of culture solution can be kept to be higher than ambient temperature in winter, the temperature of culture solution can also be made less than outer in summer
The temperature of boundary's environment realizes uniform illumination condition, long-term constant temperature incubation, carries out haematococcus pluvialis work in northern area to meet
The demand of industry culture.By using this system, haematococcus pluvialis culture-liquid temp can be enable to be maintained at 20 DEG C~28 throughout the year
DEG C, while northern substantial light being made full use of to shine, so that algae solution is obtained uniform illumination.
Description of the drawings
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is haematococcus pluvialis variable density figure under 28 DEG C of cultivation temperatures, different illumination intensities；
Fig. 2 is haematococcus pluvialis variable density figure under 24 DEG C of cultivation temperatures, different illumination intensities；
Fig. 3 is haematococcus pluvialis variable density figure under 20 DEG C of cultivation temperatures, different illumination intensities；
Fig. 4 is haematococcus pluvialis variable density figure under 16 DEG C of cultivation temperatures, different illumination intensities；
Fig. 5 is haematococcus pluvialis variable density figure under 12 DEG C of cultivation temperatures, different illumination intensities；
Fig. 6 is that intensity of illumination is 30 μm of ol m-2s-1Algae density with temperature's changing rule figure；
Fig. 7 is that intensity of illumination is 50 μm of ol m-2s-1Algae density with temperature's changing rule figure；
Fig. 8 is that intensity of illumination is 70 μm of ol m-2s-1Algae density with temperature's changing rule figure；
Fig. 9 is the structural schematic diagram of the culture systems of the haematococcus pluvialis for being suitable for the north of the present invention；
Reference numeral：1, inner canister；2, outer tank；3, inner cavity；4, incubation cavity is recycled；5, water inlet pipe I；6, return duct I；7, water pump
I；8, water inlet pipe II；9, return duct II；10, water pump II；11, temperature-controlled tube；12, water storage room；13, micro algae culturing device main body；14、
Main pipeline I；15, main pipeline II；16, lateral；17, control valve I；18, control valve II；19, temperature detector；20, charger；
201, E fonts branched pipe；202, vertical tube is inflated；203, transverse tube is inflated；21, head cover；22, the dirt bed area of coverage；23, tubular service
Area；24, sealing thermal insulation plate；25, thermal-collecting tube.
Specific implementation mode
The method of the present invention is described in detail with reference to specific embodiment.
Embodiment 1
The influence of intensity of illumination and temperature to haematococcus pluvialis growing：
1, material
Haematococcus pluvialis algae comes from Shandong Province marine organisms research institute algae center Germplasm Bank.
BBM culture mediums:Culture medium mother liquor 1 (1000 times of concentration):Sodium nitrate 25g/100ml；Dipotassium hydrogen phosphate 7.5g/
100ml, potassium dihydrogen phosphate 17.5g/100ml；Culture medium mother liquor 2 (1000 times of concentration) vitamin B1:1g/L；Biotin:
0.25mg/L；Vitamin B12:0.15mg/L.Culture medium mother liquor 3 (1000 times of concentration) disodium salt disodium (Na2EDTA)：
0.75g/L；Tetrahydrate manganese chloride (MnCl2.4H2O)：0.041g/L；Seven water zinc chloride (ZnCl2.7H2O)：0.005g/L；Molybdate dihydrate
Sour sodium (Na2MoO4.2H2O)：0.004g/L；Ferric chloride (FeCl36H2O) (FeCl3.6H2O)：0.097g/L；CoCL2 6H2O (CoCl2,
6H2O)：0.002g/L.When culture, mother liquor is diluted into 1000 times of uses.
2, test method
5 temperature gradients are set:12 DEG C, 16 DEG C, 20 DEG C, 24 DEG C and 28 DEG C.Intensity of illumination is respectively 30 μm of ol m-2s-1,
50μmol m-2s-1, 70 μm of ol m-2s-1, periodicity of illumination 12L: 12D.Every group of difference variation is ± 1 DEG C.Initially algae solution density is
3*103A/mL measures algae density after 7d, 14d, 21d.
The measurement of algae density：The counting of algae is carried out with blood counting chamber, every bottle is surveyed 5 times, its average value is taken.
The measurement of growth rate：It is green that algae live body leaf is measured in the case where wavelength is 674nm with the ultraviolet grating spectrophotometer of 752 types
The light absorption value A674 of element, the algae density measured as this time according to the average value of 3 parallel groups.The following public affairs of growth rate (μ)
Formula calculates:
μ=(lnNt2-lnNt1)/(t2-t1)
(1) in formula, μ is growth rate, Nt2For t2The A674 values of time, Nt1For t1The A674 values of time.
As shown in figures 1-8,30 μm of ol m-2s-1Be conducive to the growth of algae, 50 μm of ol m-2s-1With 70 μm of ol m-2s-1Light intensity
It is unfavorable for the growth of algae for a long time, but intensity of illumination is stronger, the time that algae reaches light intensity highest algae density is shorter.
28 DEG C are compared, 24 DEG C of growths for being more suitable for algae are 70 μm of ol m in intensity of illumination in 21d-2s-1When, algae is close
There is a degree of decline in degree, illustrates at 24 DEG C, 70 μm of ol m-2s-1It is not still the light intensity of a suitable long-term cultivation.
When cultivation temperature is 20 DEG C, opposite to play other cultivation temperatures, the speed of algae solution density growth is affected by temperature to compare light intensity
Be affected, algae solution does not show density downward trend in the 21d limited times.Relative to other experimental groups,
When 21d, in 30 μm of ol m-2s-1Under, algae density reaches experiment peak.Temperature in a certain range, shadow of the light intensity to algae density
It rings it is obvious that wherein 30 μm of ol m-2s-1When can reach higher algae density, 50 and 70 μm of ol m-2s-1Culture can make algae for a long time
The a degree of loss of density, but in a short time, when algae density is little, light intensity can be such that by force algae is improved in the shorter time a bit
The concentration of algae.
During cultivating microalgae, extraneous contamination is that far-reaching one of the success or failure of a culture to microalgae are asked
Topic, in addition to paying attention to Experimental Procedures during practical operation, avoids other than pollution, the breeding for keeping microalgae as fast as possible as possible
As the sociales in culture solution be also one common recognition the problem of.When cultivation temperature is very low, the breeding of haematococcus pluvialis is slower,
And the growth of other pollutants is very fast, cracking completely finishes death to frustule when temperature is 12 DEG C, after cultivating 21d, is training
It can not find in nutrient solution.Therefore in the actual production process, algae solution cannot be cultivated for a long time in the environment of less than 12 DEG C.
Temperature has synergistic effect in a certain range with light intensity.When temperature is 16 DEG C, intensity of illumination is 70 μm of ol m-2s-1, it is 28 DEG C with temperature, intensity of illumination is 30 μm of ol m-2s-1When, algae density difference is little.Therefore in the actual production process, such as
Under conditions of when fruit temperature is higher, frustule can be given birth to offset a part of intensity of illumination by suitably reducing the intensity of illumination of algae
Long adverse effect.In the growth conditions of algae, temperature influences very big with intensity of illumination, wherein when temperature is 24 DEG C, illumination is strong
Degree is 30 μm of ol m-2s-1When, algae density is maximum.
In summary, suitable for by the control of the cultivation temperature of haematococcus pluvialis, between 20-28 DEG C, especially 24 DEG C are advisable.
Embodiment 2
As shown in figure 9, a kind of culture systems for the haematococcus pluvialis being suitable for the north, including underground microdisk electrode tank,
Upper solar energy water-storing device and ground micro algae culturing device.The main body of the underground microdisk electrode tank uses stainless steel material, institute
State the double layers of tank body structure that underground microdisk electrode tank is made of inner canister 1 and outer tank 2, outer wall, inner wall and the institute of the outer tank 2
The outer wall surface for stating inner canister 1 is all made of spray zinc-aluminium technique, then spray paint, and the inner wall surface of the inner canister 1 is equipped with ceramics and applies
Layer (not shown).Cycle incubation cavity 4 is equipped between the outer tank 2 and inner canister 1.It the ground solar energy water-storing device and follows
It is connected respectively with return duct I 6 by water inlet pipe I 5 between ring incubation cavity 4, the water inlet pipe I 5 is equipped with flow from following
Ring incubation cavity 4 is pumped into the water pump I 7 of ground solar energy water-storing device, and the return duct I 6 is equipped with control valve I 17.It is described into
The bottom of water pipe I 5 is close to 4 bottom of cycle incubation cavity, to make the water layer of 4 bottom of cycle incubation cavity can also pass through the ground sun
Energy water-storing device carries out heat exchange, whole unanimously and without the temperature difference to ensure to recycle the temperature in incubation cavity 4, and then is to ensure
The algae solution of inner cavity 3 growth and breeding at a temperature of homogeneous constant.
The inner cavity 3 of the inner canister 1 being internally provided with for cultivating haematococcus pluvialis, the inner cavity 3 and ground of the inner canister 1 are micro-
Algae culturing device is connected by water inlet pipe II 8 with return duct II 9 respectively, and the water inlet pipe II 8 is equipped with gives birth to red ball by rain
Algae is pumped into the water pump II 10 of ground micro algae culturing device from inner cavity 3, and the return duct II 9 is equipped with control valve II 18.It is described
The bottom of water inlet pipe II 8 is close to the bottom of inner cavity 3, to make the algae solution of bottom in inner cavity 3 can also pass through ground microdisk electrode dress
It sets and fully carries out photosynthesis, and then ensure whole haematococcus pluvialis healthy growths.
The underground microdisk electrode tank locates below ground level, and the bottom of the inner canister 1 is located at 5m-8m from the ground, described
The bottom of outer tank 2 is located at 6m-9m from the ground.The top of the inner canister 1 is equipped with head cover 21, the water inlet pipe II 8 and return duct
II 9 can pass through to be connected to the inner cavity 3 and ground micro algae culturing device from head cover 21, and the over top of inner canister 1 is except necessary
Remaining outer position of pipeline is also closed.The top of the head cover 21 is equipped with the dirt bed area of coverage 22 and tubular service area 23, institute
The thickness for stating the dirt bed area of coverage 22 is 3-5m, is covered without soil at the tubular service area 23, the face in the tubular service area
Product is 0.5x0.5m2, the side wall in the tubular service area 23 is used to carry out through spraying the stainless steel (not shown) of zinc-aluminium processing
Fixed, the channel formed is used for away pipeline and operating personnel carry out pipeline maintenance use.The top in the tubular service area 23
End cap is equipped with sealing thermal insulation plate 24, and the sealing thermal insulation plate 24 includes stainless steel plate and the plastic-spraying for being fixed on stainless steel surface
Adiabator layer (not shown).
Temperature detector 19 is equipped in the cycle incubation cavity 4 and/or inner cavity 3 of inner canister 1, in the cycle incubation cavity 4
Temperature detector 19 be tightly attached on the outer wall of inner canister 1.Charger 20, the charger are additionally provided in the inner cavity 3 of the inner canister 1
20 be inverted T font, and the charger 20 is set including E fonts branched pipe 201, vertically disposed inflation vertical tube 202 and level
The inflation transverse tube 203 set, the inflation transverse tube 203 are mounted on the bottom of inflation vertical tube 202, and the E fonts branched pipe 201 is two
Group, is separately mounted to the left and right ends of inflation transverse tube 203, and the E fonts branched pipe 201 will flow through inflation vertical tube equipped with multiple
202, the stomata that the air-flow of inflation transverse tube 203 finally discharges.The inflation vertical tube 202 is also worn from tubular service area 23 and head cover 21
It crosses.0~0.5VVM of gas pushing quantity of the charger 20 is 0~3kg/cm to keep the pressure of inner cavity 32。
The ground solar energy water-storing device includes temperature-controlled tube 11, thermal-collecting tube 25 and the water storage being connected with thermal-collecting tube 25
Room 12 is connected by water inlet pipe I, return duct I respectively between the water storage room 12 and cycle incubation cavity 4.When ambient temperature mistake
When low, circulating between incubation cavity 4 and ground solar energy water-storing device can be being recycled by flow, to recycle incubation cavity 4
Continue heat supply, to ensure the algae culture temperature in inner cavity 3.
The ground micro algae culturing device includes several micro algae culturing device main bodys 13 and microalgae in communication training
Nutrient solution circulating line, the micro algae culturing device main body 13 use colume type transparent polybag.The micro algae culturing liquid circulating line
Including multiple laterals 16 and main pipeline I 14 arranged in parallel and main pipeline II 15, the main pipeline I 14 is located at master
The top of pipeline II 15；The micro algae culturing device main body 13 is 1-10 (preferably 4) and vertical placement, each microalgae
Top and bottom end are connected by a lateral 16 with main pipeline I 14 and main pipeline II 15 respectively in culture apparatus main body 13.
The main pipeline I 14 is being equipped with the ports A close to one end of water inlet pipe II 8, the other end be equipped with for add algae, culture medium with
And the ports D of water；The main pipeline II 15 is being equipped with the ports B close to one end of return duct II 9, and the other end, which is equipped with, is used for algae solution
The C-terminal mouth of recycling.When normal use, by two port closeds of C, D, the algae solution of haematococcus pluvialis is along water inlet pipe II 8 from the ports A
It is pumped into main pipeline I 14 and then branches to each micro algae culturing device main body 13 along each lateral 16 due to gravity, because of institute
Colume type transparent polybag of the micro algae culturing device main body 13 using light-permeable is stated, algae can carry out photosynthesis, then, algae herein
Liquid due to gravity, continues to decline, and is collected in main pipeline II 15 with each lateral 16, then passes through the ports B, along reflux
Pipe II 9 is back in the inner cavity 3 of the inner canister 1 of underground.By the keying of water pump II 10 and control valve 18, adjustment algae solution is in column
The residence time of formula transparent plastic bag can also realize algae solution incessantly in underground microdisk electrode tank and ground micro algae culturing device
It circulates.
The present invention also provides the application methods of the culture systems of the haematococcus pluvialis for being suitable for the north comprising
Following steps：
(1) environment temperature more than ground is at 20-28 DEG C, the haematococcus pluvialis only interior progress of algae culturing device on the ground
Culture；
(2) when the environment temperature more than ground is 28 DEG C high, it is micro- that the micro algae culturing liquid containing haematococcus pluvialis imports underground
It is cultivated in algae culture tank, and is pumped into the micro algae culturing device of ground by the cycles of water pump II 10 and carries out photosynthesis；
(3) when environment temperature is less than 20 DEG C, the micro algae culturing liquid containing haematococcus pluvialis imports underground microdisk electrode tank
In cultivated, the water circulation between being recycled by ground solar energy water-storing device and cycle incubation cavity 4, control inner canister 1
Temperature in inner cavity 3 heats the micro algae culturing liquid in inner cavity 3 to 20-28 DEG C, and will periodically contain haematococcus pluvialis
Micro algae culturing liquid is pumped into the micro algae culturing device of ground by the cycles of water pump II 10 and carries out photosynthesis.Be specifically shown in embodiment 3,
4、5。
Culture light intensity is controlled by way of being covered with straw screen or mat to ground micro algae culturing device, keeps illumination strong
Degree is maintained at 20~50 μm of ol m-2s-1, optimal is 30 μm of ol m-2s-1。
Embodiment 3
Use overall diameter for 1.5m, interior diameter 1.3m, high 2m (VIt is interior=2.65m3) set tank simulate underground culture tank,
It is embedded in underground 4m or so, and 12 DEG C of outdoor mean temperature covers 20 DEG C of mean temperature in tank.
Ground algae culturing device uses pillar bag culture (being cultivated with colume type transparent polybag), each bag 80L total
There are 20, culture solution VLiquid=1.5m3.Inoculum density is 1x103A/mL.Open the control valve II on return duct II, ground microalgae
Micro algae culturing liquid in culture apparatus is because gravity passes through in inflow inner canister；The lower end of water inlet pipe II extends to the bottom of inner canister
Portion opens control valve II and water pump II, and the flow velocity of control water pump II is 2L/min, makes micro algae culturing liquid algae culturing device on the ground
It is recycled between the culture tank of underground.Air is passed through to culture pot bottom by inflation system simultaneously during culture, gas pushing quantity 0~
0.5VVM keeps 0~3kg/cm of pressure tank in culture tank2。
Quantity of circulating water between ground solar energy water-storing device volume 1000L, with cycle incubation cavity is 0.8m3.It opens
Control valve I, the water in the solar energy water-storing device of ground are flowed into due to gravity in cycle incubation cavity；The lower end of water inlet pipe I extends to
The bottom of incubation cavity is recycled, control valve I and water pump I is opened, the flow velocity of control water pump I makes the temperature in cycle incubation cavity at 20 DEG C
Between~28 DEG C.Simultaneously individually with identical ground culture apparatus culture haematococcus pluvialis as a contrast.
After cultivating 2 weeks, by a concentration of 5x10 of the experimental group haematococcus pluvialis of the system and device culture of the present invention5A/mL,
A concentration of 7x10 of control group4A/mL, experimental group is 7 times of control group culture as a result,.
Embodiment 4
Use overall diameter for 1.5m, interior diameter 1.3m, high 2m (2.65m3) set tank simulate underground culture tank, bury
In underground 4m or so, 33 DEG C of outdoor temperature covers 22 DEG C of temperature in tank.
Ground algae culturing device uses pillar bag culture, each bag 80L to share 20, culture solution VLiquid=1.5m3.Inoculation
A concentration of 1x103A/mL.Open the control valve II on return duct II, the micro algae culturing liquid in the micro algae culturing device of ground because
Gravity is by flowing into inner canister；The lower end of water inlet pipe II extends to the bottom of inner canister, opens control valve II and water pump II, control
The flow velocity of water pump II is 2L/min, and micro algae culturing liquid is made to be recycled between algae culturing device and underground culture tank on the ground.During culture
Air is passed through to culture pot bottom by inflation system simultaneously, 0~0.5VVM of gas pushing quantity, keep pressure tank 0 in culture tank~
3kg/cm2。
Simultaneously with identical independent culture apparatus in outside scenery haematococcus pluvialis as a contrast.
After cultivating 2 weeks, a concentration of 3x10 of haematococcus pluvialis in system5/ mL, a concentration of 1x10 of control group4A/mL, 2 weeks
Afterwards, system throughput of the invention is 30 times of control group culture.The haematococcus pluvialis of control group culture, intensity of illumination are equal with temperature
Excessively high, frond turns yellow, and gradually reddens, and precipitates.It withers away after 2 months.
Embodiment 5
Use overall diameter for 1.5m, interior diameter 1.3m, high 2m (2.65m3) set tank simulate underground culture tank, bury
In underground 4m or so, 5 DEG C of outdoor temperature covers 16 DEG C of temperature in tank.
Ground algae culturing device uses pillar bag culture, each bag 80L to share 20, culture solution VLiquid=1.5m3.Inoculation
A concentration of 1x103A/mL.Open the control valve II on return duct II, the micro algae culturing liquid in the micro algae culturing device of ground because
Gravity is by flowing into inner canister；The lower end of water inlet pipe II extends to the bottom of inner canister, opens control valve II and water pump II, makes micro-
Algae culturing liquid recycles between algae culturing device and underground culture tank on the ground.The flow velocity for controlling water pump II is 4L/min, turn on pump when
Between 10h~16h/d, other times close cycle.Air is passed through to culture pot bottom by inflation system simultaneously during culture, is sent
0~0.5VVM of tolerance keeps 0~3kg/cm of pressure tank in culture tank2。
Quantity of circulating water between ground solar energy water-storing device volume 1000L, with cycle incubation cavity is 0.8m3.It opens
Control valve I, the water in the solar energy water-storing device of ground are flowed into due to gravity in cycle incubation cavity；The lower end of water inlet pipe I extends to
The bottom of incubation cavity is recycled, control valve I and water pump I is opened, the flow velocity of control water pump I makes the temperature in cycle incubation cavity at 20 DEG C
Between~28 DEG C.Simultaneously individually with identical ground culture apparatus culture haematococcus pluvialis as a contrast.
After cultivating 2 weeks, a concentration of 1x10 of haematococcus pluvialis in system5A/mL, a concentration of 1x10 of control group2A/mL, and
When outdoor temperature drops to 12 DEG C or less, control group culture was that all algaes are all dead less than one month.Therefore, outdoor temperature
Too low, haematococcus pluvialis cannot be grown, moreover, other bacteriums but occupy advantage, haematococcus pluvialis be made gradually to wither away.
The foregoing is merely the preferred embodiments of invention, are not intended to limit the invention, all spirit in the present invention
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of culture systems for the haematococcus pluvialis being suitable for the north, it is characterised in that：It include the ground more than ground
Solar energy water-storing device and ground micro algae culturing device and the underground microdisk electrode tank to locate below ground level, the underground microalgae
The double layers of tank body structure that culture tank is made of inner and outer tank, the inner canister are internally provided with for cultivating haematococcus pluvialis
Inner cavity is equipped with cycle incubation cavity between the outer tank and inner canister；Between the ground solar energy water-storing device and cycle incubation cavity
It is connected respectively with return duct I by water inlet pipe I, the water inlet pipe I, which is equipped with, is pumped into flow on the ground too from cycle incubation cavity
The water pump I of positive energy water-storing device；The inner cavity of the inner canister passes through water inlet pipe II and return duct respectively with ground micro algae culturing device
II is connected, and the water inlet pipe II is equipped with the water pump II that haematococcus pluvialis is pumped into ground micro algae culturing device from inner cavity.
2. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The inner canister
Top be equipped with head cover, the water inlet pipe II and return duct II can pass through to be connected to the inner cavity and the training of ground microalgae from head cover
Support device；The top of the head cover be equipped with the dirt bed area of coverage and tubular service area, the tubular service area be used for away pipeline with
And tubular service, the top cover in the tubular service area are equipped with sealing thermal insulation plate, the sealing thermal insulation plate include stainless steel plate with
And it is fixed on the plastic-spraying adiabator layer of stainless steel surface.
3. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The ground
Solar energy water-storing device includes temperature-controlled tube, thermal-collecting tube and the water storage room being connected with thermal-collecting tube, and the water storage room and cycle are protected
It is connected respectively by water inlet pipe I, return duct I between warm chamber.
4. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The ground
Micro algae culturing device includes several micro algae culturing device main bodys and micro algae culturing liquid circulating line in communication；It is described micro-
Algae culturing device main body uses colume type transparent polybag.
5. the culture systems of the haematococcus pluvialis according to claim 4 for being suitable for the north, it is characterised in that：The microalgae
Culture solution circulating line includes multiple laterals and main pipeline I arranged in parallel and main pipeline II, and the main pipeline I is located at
The top of main pipeline II；The micro algae culturing device main body is 4 and vertical placement, top in each micro algae culturing device main body
It is connected respectively with main pipeline I, main pipeline II by a lateral with bottom end；The main pipeline I is close to the one of water inlet pipe II
End is equipped with the ports A, and the other end is equipped with the ports D for adding algae, culture medium and water；The main pipeline II is close to reflux
One end of pipe II is equipped with the ports B, and the other end is equipped with the C-terminal mouth for algae solution recycling.
6. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The inner canister
Bottom be located at 5m-8m from the ground and locate, the bottom of the outer tank is at 6m-9m from the ground；The thickness of the dirt bed area of coverage
Degree is 3-5m；The return duct I is equipped with control valve I；The return duct II is equipped with control valve II；The bottom of the water inlet pipe I
Portion is close to cycle incubation cavity bottom, the bottom of the bottom of the water inlet pipe II close to inner cavity；It is in the cycle incubation cavity and/or interior
Temperature detector is equipped in the inner cavity of tank, the temperature detector in the cycle incubation cavity is tightly attached on the outer wall of inner canister.
7. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The inner canister
Interior intracavitary be additionally provided with charger；The charger is inverted T font, and the charger includes E fonts branched pipe, vertical
The inflation vertical tube of setting and horizontally disposed inflation transverse tube, the inflation transverse tube are mounted on the bottom of inflation vertical tube, the E words
Shape branched pipe is two groups, is separately mounted to the left and right ends of inflation transverse tube, and the E fonts branched pipe will be filled equipped with multiple will flow through
The stomata that gas vertical tube, the air-flow for inflating transverse tube finally discharge；0~0.5VVM of gas pushing quantity of the charger, in keeping
The pressure of chamber is 0~3kg/cm2。
8. the culture systems of the haematococcus pluvialis according to claim 1 for being suitable for the north, it is characterised in that：The underground
The main body of microdisk electrode tank uses stainless steel material；The outer wall surface of the outer wall of the outer tank, inner wall and the inner canister is adopted
With spray zinc-aluminium technique, then spray paint；The inner wall surface of the inner canister is equipped with ceramic coating.
9. it is suitable for the application method of the culture systems of the haematococcus pluvialis in the north according to claim 1-8 any one of them,
It is characterized by comprising the following steps：
(1) environment temperature more than ground is at 20-28 DEG C, and haematococcus pluvialis is only trained in algae culturing device on the ground
It supports；
(2) when the environment temperature more than ground is 28 DEG C high, the micro algae culturing liquid containing haematococcus pluvialis imports the training of underground microalgae
It supports and is cultivated in tank, and be pumped into the micro algae culturing device of ground by water pump II cycles and carry out photosynthesis；
(3) when environment temperature is less than 20 DEG C, micro algae culturing liquid containing haematococcus pluvialis import in the microdisk electrode tank of underground into
Row culture controls the interior intracavitary of inner canister by the water circulation between ground solar energy water-storing device and cycle incubation cavity cycle
Temperature to 20-28 DEG C, the micro algae culturing liquid in inner cavity is heated, and periodically by the microdisk electrode containing haematococcus pluvialis
Liquid is pumped into the micro algae culturing device of ground by water pump II cycles and carries out photosynthesis；
Culture light intensity is controlled by way of being covered with straw screen or mat to ground micro algae culturing device, intensity of illumination is made to protect
It holds in 20~50 μm of ol m-2s-1。
10. the application method of the culture systems of the haematococcus pluvialis according to claim 9 for being suitable for the north, feature exist
In：The intensity of illumination is maintained at 30 μm of ol m-2s-1。
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