CN108265058B - 一种重组粉尘螨1类变应原蛋白及其制备方法和应用 - Google Patents
一种重组粉尘螨1类变应原蛋白及其制备方法和应用 Download PDFInfo
- Publication number
- CN108265058B CN108265058B CN201711379684.0A CN201711379684A CN108265058B CN 108265058 B CN108265058 B CN 108265058B CN 201711379684 A CN201711379684 A CN 201711379684A CN 108265058 B CN108265058 B CN 108265058B
- Authority
- CN
- China
- Prior art keywords
- proder
- protein
- gene
- der
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43531—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from mites
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/35—Allergens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pulmonology (AREA)
- Botany (AREA)
- Water Supply & Treatment (AREA)
Abstract
Description
Claims (6)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2016112672507 | 2016-12-31 | ||
CN201611267250 | 2016-12-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108265058A CN108265058A (zh) | 2018-07-10 |
CN108265058B true CN108265058B (zh) | 2020-12-22 |
Family
ID=62710868
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711379684.0A Active CN108265058B (zh) | 2016-12-31 | 2017-12-20 | 一种重组粉尘螨1类变应原蛋白及其制备方法和应用 |
Country Status (3)
Country | Link |
---|---|
US (1) | US10975128B2 (zh) |
CN (1) | CN108265058B (zh) |
WO (1) | WO2018121639A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109456989B (zh) * | 2018-10-31 | 2022-03-29 | 陕西慧康生物科技有限责任公司 | 一种提高毕赤酵母分泌表达的载体的构建方法 |
CN111595971B (zh) * | 2020-05-22 | 2021-06-15 | 浙江大学 | 一种灰尘中尘螨过敏原蛋白Der f1和Der p1及其硝化产物的检测方法 |
CN117362453B (zh) * | 2023-12-08 | 2024-02-27 | 山东硕景生物科技有限公司 | 一种Derf1重组抗原、其制备方法及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1519949A1 (de) * | 2002-07-10 | 2005-04-06 | MERCK PATENT GmbH | Varianten des hausstaubmilbenallergens der f 2 |
CN102676568A (zh) * | 2012-05-08 | 2012-09-19 | 崔玉宝 | 一种生产重组粉尘螨变应原Der f1和Der f2融合蛋白的方法 |
CN108265056A (zh) * | 2016-12-31 | 2018-07-10 | 江苏众红生物工程创药研究院有限公司 | 一种重组户尘螨1类变应原蛋白及其制备方法和应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU7878900A (en) * | 1999-10-15 | 2001-04-30 | Heska Corporation | Method for production and use of mite group 1 proteins |
CN1780852A (zh) * | 2003-04-25 | 2006-05-31 | 诺和酶股份有限公司 | 组1螨多肽变体 |
-
2017
- 2017-12-20 CN CN201711379684.0A patent/CN108265058B/zh active Active
- 2017-12-28 WO PCT/CN2017/119190 patent/WO2018121639A1/zh active Application Filing
-
2019
- 2019-06-28 US US16/456,357 patent/US10975128B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1519949A1 (de) * | 2002-07-10 | 2005-04-06 | MERCK PATENT GmbH | Varianten des hausstaubmilbenallergens der f 2 |
CN102676568A (zh) * | 2012-05-08 | 2012-09-19 | 崔玉宝 | 一种生产重组粉尘螨变应原Der f1和Der f2融合蛋白的方法 |
CN108265056A (zh) * | 2016-12-31 | 2018-07-10 | 江苏众红生物工程创药研究院有限公司 | 一种重组户尘螨1类变应原蛋白及其制备方法和应用 |
Non-Patent Citations (3)
Title |
---|
Expression and refolding of mite allergen pro-Der f1 from inclusion;Chunfang Ling;《Protein Expression and Purification》;20141224(第109期);第93-98页 * |
尘螨变应原Der f1全序列的原核表达及生物信息学分析;崔玉宝等;《四川动物》;20080130;第27卷(第01期);第37-43页 * |
屋尘螨重组变应原Der p 2的表达、纯化及免疫学活性鉴定;刘晓宇等;《中国人兽共患病学报》;20090815;第25卷(第08期);第764-767页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108265058A (zh) | 2018-07-10 |
US20190389919A1 (en) | 2019-12-26 |
WO2018121639A1 (zh) | 2018-07-05 |
US10975128B2 (en) | 2021-04-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11359191B2 (en) | Variant recombinant dermatophagoides pteronyssinus type 1 allergen protein and its preparation method and application | |
CN108350447B (zh) | 启动子变体 | |
CN108265058B (zh) | 一种重组粉尘螨1类变应原蛋白及其制备方法和应用 | |
CN109280656B (zh) | 重组布氏白僵菌蛋白酶k突变体pk-m1及制备方法 | |
CN104232611B (zh) | 一种重组布氏白僵菌蛋白酶k及其工业化生产与纯化方法 | |
CN111041033A (zh) | 重组人生长激素及其真核系统表达方法 | |
CN110628738B (zh) | 提高葡萄糖氧化酶活性的方法、突变体及其应用 | |
CN109913380B (zh) | 生产(-)-α-红没药醇的重组解脂耶氏酵母菌及其构建方法和应用 | |
CN105802989B (zh) | 毕赤酵母表达重组蛋白的载体、基因、方法及应用 | |
CN108265059B (zh) | 一种重组粉尘螨2类变应原蛋白及其制备方法与应用 | |
CN109207460B (zh) | 重组布氏白僵菌蛋白酶k突变体pk-m2及制备方法 | |
CN107778365B (zh) | 一种多点整合的重组蛋白表达方法 | |
CN113797326B (zh) | 一种预防冠状病毒引起疾病的疫苗 | |
US11319353B2 (en) | Recombinant Dermatophagoides pteronyssinus type 2 allergen protein and its preparation method and application | |
CN108265065B (zh) | 一种重组黄花蒿1类变应原蛋白及其应用 | |
JP4491588B2 (ja) | キラー蛋白質の精製方法 | |
CN109609539B (zh) | 一种从重组毕赤酵母中制备elabela多肽的方法 | |
CN117247911B (zh) | 大肠杆菌dna连接酶突变体及其在毕赤酵母中表达纯化方法 | |
CA3155541A1 (en) | Nucleic acids, vectors, host cells and methods for production of fructosyltransferase from aspergillus japonicus | |
CN111909916A (zh) | 一种来源于南极磷虾的双链特异性核酸酶及其制备方法 | |
CN116622784B (zh) | 一种大麻二酚酸合成酶的应用 | |
CN110564746A (zh) | 一种耐酸型单宁酶及其基因和应用 | |
CN111004318B (zh) | rhPTH(1-34)蛋白原液的纯化方法 | |
Wang et al. | Expression and purification of beefy meaty peptide in Pichia pastoris | |
CN110951634A (zh) | 大黄鱼il-4/13a基因毕赤酵母表达产物制备方法及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 213125 No. 518 Yunhe Road, Xinbei District, Changzhou City, Jiangsu Province Applicant after: ZonHon Biopharma Institute Inc. Applicant after: Jiangsu Jingsen Biomedical New Materials Technology Co., Ltd. Address before: 213125 No. 518 Yunhe Road, Xinbei District, Changzhou City, Jiangsu Province Applicant before: ZonHon Biopharma Institute Inc. Applicant before: Changzhou Gensun Institute of Biomedicine Co., Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20190711 Address after: 213125 No. 518 Yunhe Road, Xinbei District, Changzhou City, Jiangsu Province Applicant after: ZonHon Biopharma Institute Inc. Address before: 213125 No. 518 Yunhe Road, Xinbei District, Changzhou City, Jiangsu Province Applicant before: ZonHon Biopharma Institute Inc. Applicant before: Jiangsu Jingsen Biomedical New Materials Technology Co., Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant |