CN108250318A - A kind of preparation method of dictyophora phalloidea proteoglycan - Google Patents
A kind of preparation method of dictyophora phalloidea proteoglycan Download PDFInfo
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- CN108250318A CN108250318A CN201810182782.3A CN201810182782A CN108250318A CN 108250318 A CN108250318 A CN 108250318A CN 201810182782 A CN201810182782 A CN 201810182782A CN 108250318 A CN108250318 A CN 108250318A
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- dictyophora phalloidea
- proteoglycan
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to the method that dictyophora fungus polysaccharide is extracted using dictyophora phalloidea as raw material, especially with respect to using remaining leftover pieces after the dry dictyophora phalloidea processing of commodity for the preparation method of raw material extraction dictyophora phalloidea proteoglycan.This method is using dry dictyophora phalloidea as raw material, and by coarse crushing, hot water extraction, coarse filtration, clarification refined filtration, decoloration, vacuum concentration, alcohol precipitation, alcohol hypostasis is dry, crush, sieving can be prepared by dictyophora phalloidea proteoglycan.This production technology be easy to corresponding coordinative composition of equipments, therefore can be more convenient to realize merchandized handling, the dictyophora phalloidea proteoglycan produced has apparent antitumor activity, and to the inhibiting effect of murine sarcoma S 180, inhibiting rate is 48.12% 62.25%.Therefore the polysaccharide can be made into for the dedicated oral biological preparation of tumour Radiotherapy chemotherapy patient.
Description
Technical field
The present invention relates to using dictyophora phalloidea as the method for raw material extraction dictyophora fungus polysaccharide, add especially with respect to the dry dictyophora phalloidea of commodity
Remaining leftover pieces extract the preparation method of dictyophora phalloidea proteoglycan for raw material after work.
Background technology
By in recent years the study found that dictyophora phalloidea groups such as amino acid, vitamin, polysaccharide and plurality of inorganic salt containing there are many
Point.In addition to nutritive value, also with important disease-prevention health effect.Polysaccharide is constituent important in dictyophora phalloidea fructification
One of, it has important bioactive functions.Application No. is 96115300.8《A kind of preparation side of dictyophora fungus polysaccharide
Method》Patent application in, disclose leftover bits and pieces or submerged fermentation technology that a kind of cap, volva and dictyophora phalloidea using dictyophora phalloidea is processed
The dictyophora phalloidea vegetative mycelium of production is raw material, and the master operations such as preprocessed, hot-water extraction, alcohol analysis, dialysis and washing are made slightly
Polysaccharide and further by Thick many candies by DEAE- cellulose columns carry out chromatography obtain the method for high-purity dictyophora fungus polysaccharide.
Application No. is 200810197136.0《A kind of extracting method of triple helix Dictyophora phalloidea polysaccharide》Patent application
In, disclose it is a kind of first by the degreasing in cable type extractor according with organic solvent of the natural dictyophora phalloidea after crushing, then by residue
Successively
Water carries under 20-80 DEG C of gradient temperature, filters to get filtrate, and repeats three times, centrifuging and taking supernatant, then through Sevage
After method takes off albumen and decolourized with H2O2 oxidizing process, dialyse to remove small molecule, and pass through with clear water and redistilled water respectively
After DEAE columns remove acidic polysaccharose, solution freeze-drying obtains the method for the triple helix Dictyophora phalloidea polysaccharide that form is flocculent white.
Have no the report about dictyophora phalloidea proteoglycan and preparation method thereof.
Invention content
The object of the present invention is to provide one kind dictyophora phalloidea egg is extracted as raw material using remaining leftover pieces after the dry dictyophora phalloidea processing of commodity
The preparation method of white polysaccharide.
The purpose of the present invention realize technical solution be：Using dry dictyophora phalloidea as raw material, by coarse crushing, hot water extraction, coarse filtration, clear
Clear refined filtration, decoloration, vacuum concentration, alcohol precipitation, alcohol hypostasis is dry, crushes, sieving.
Dry dictyophora phalloidea used in the present invention can be remaining leftover pieces after the dry dictyophora phalloidea processing of commodity.
The design concept of the present invention is focused on to realize merchandized handling, so extracting dictyophora phalloidea albumen in the method for the present invention
During polysaccharide, dry dictyophora phalloidea raw material should first be made appropriate coarse crushing first, to improve the leaching rate of polysaccharide, but raw material cannot be crushed
Carefully, it is responsible for leaching liquor to glue excessively and be difficult to filter；The water of extraction is larger, is 40~50 times of dry dictyophora phalloidea raw material weight,
80 °~90 DEG C of extraction temperature, extraction time 4~8 hours and after extracting are centrifuged the filter residue of discharge, and make heat
Water soaked overnight takes this soak to be used as the extraction of the dry dictyophora phalloidea raw material of next day next group, therefore can improve the leaching of dictyophora fungus polysaccharide
Extracting rate, leaching process are once completed, simple and practicable；The leaching liquor after coarse filtration is centrifuged, makees clarifying treatment with a kind of albumen,
Not only safety, and clarifying effect is notable, improves the speed of refined filtration thereafter, effectively alleviates the most difficult in whole preparation process
Filtration problem；Make decolorization with 1-2% H2O2；Clear and bright leaching liquor is after vacuum concentration, soluble solid control
Final ethanol concentration control at 10~30%, alcohol precipitation can be such that dictyophora fungus polysaccharide once obtains completely 80~90%
Alcohol is analysed, it is not necessary to be made multiple alcohol precipitation, be further simplified technical process.
The filter residue of discharge is centrifuged, then is impregnated with pure water, when soak feeds intake as the dry dictyophora phalloidea raw material of next group
Extraction use.The filter residue isolated at this time is used to produce the health food of high microsteping content.
With the dictyophora phalloidea proteoglycan prepared by the preparation method of this dictyophora phalloidea proteoglycan provided by the invention, by oral
Inhibiting effect research has been carried out to murine sarcoma S-180 with two kinds of approach of intraperitoneal injection, it is with obvious effects.
Table 1 takes orally operative condition table of the dictyophora phalloidea proteoglycan to murine sarcoma S-180：
Effect of the dictyophora phalloidea proteoglycan to murine sarcoma S-180 is injected intraperitoneally in table 2：
The advantages of preparation method of this dictyophora phalloidea proteoglycan provided by the invention is：Production technology is easy to match with corresponding equipment
Set, therefore can be more convenient to realize merchandized handling.The dictyophora phalloidea proteoglycan of production has apparent antitumor activity, to small rat meat
The inhibiting effect of knurl S-180, inhibiting rate are 48.12% -62.25%.Therefore the polysaccharide can be made into for tumour Radiotherapy chemotherapy
The dedicated oral biological preparation of patient.
Specific embodiment
By 25 kilograms of dry dictyophora phalloidea coarse crushings, immediately with dry dictyophora phalloidea raw material and weight be comparably 1: 40~50, temperature
Then 80~90 DEG C of soft water, the dry dictyophora phalloidea after extraction coarse crushing 4~8 hours are centrifuged removing filter residue and obtain slightly
Filtrate, gained coarse filtration liquid make clarifying treatment with natural thermosetting property albumen, then carry out refined filtration, obtain clear and bright dictyophora phalloidea leaching liquor,
Refined filtration liquid makees decolorization with 1-2% H2O2, then carry out being concentrated in vacuo to concentration of soluble solids for 10~
30%, alcohol precipitation is carried out to concentrate plus ethyl alcohol, it is 80~90% to control final concentration of alcohol, and finally alcohol hypostasis is done
Dry, crushing, sieving obtain about 2 kilograms of dictyophora phalloidea proteoglycan.
The filter residue discharged during centrifugation, then impregnated with soft water, when soak feeds intake as the dry dictyophora phalloidea raw material of next group
Extraction use, the filter residue isolated at this time is used to produce the health food of high microsteping content.
Claims (9)
1. a kind of preparation method of dictyophora phalloidea proteoglycan, it is characterised in that：Using dry dictyophora phalloidea as raw material, soaked by coarse crushing, hot water
It carries, coarse filtration, clarification refined filtration, decoloration, vacuum concentration, alcohol precipitation, alcohol hypostasis is dry, crush, sieving can be prepared by dictyophora phalloidea proteoglycan.
2. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that：Dry dictyophora phalloidea raw material
Each inventory reaches 25 kilograms or more, and finished product recovery rate reaches >=8% in terms of dry dictyophora phalloidea raw material.
3. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that：Dry dictyophora phalloidea raw material is
Remaining leftover pieces after the dry dictyophora phalloidea processing of commodity.
4. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that：Dry bamboo through coarse crushing
Sweet-smelling grass raw material is extracted with soft water, and the weight of dry dictyophora phalloidea raw material and soft water is comparably 1: 40~50, and extraction temperature 80~
90 DEG C, extraction time 4~8 hours, leaching process is once completed.
5. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that：Dry dictyophora phalloidea raw material
Hot water extraction liquid is centrifuged after removing filter residue, and gained coarse filtration liquid makees clarifying treatment with natural thermosetting property albumen, then carries out
Refined filtration obtains clear and bright dictyophora phalloidea leaching liquor.
6. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that；Centrifuge discharge
Filter residue, then impregnated with soft water, extraction when soak feeds intake as the dry dictyophora phalloidea raw material of next group uses, and isolates at this time
Filter residue is used to produce the health food of high microsteping content.
7. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that：Refined filtration liquid 1-
2%H2O2 makees decolorization.
8. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that；Leaching liquor is through vacuum
Concentration of soluble solids after concentration is 10~30%, this concentrate adds final concentration of alcohol during ethyl alcohol alcohol precipitation to be 80
~90%, alcohol precipitation process is once completed.
9. a kind of preparation method of dictyophora phalloidea proteoglycan as described in claim 1, it is characterised in that；Dictyophora phalloidea egg obtained
White polysaccharide has the function of to inhibit tumour growth, to the tumor control rate of murine sarcoma S-180 up to 48% when oral, abdominal cavity
53-62% is reached to the tumor control rate of murine sarcoma S-180 during injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810182782.3A CN108250318A (en) | 2018-03-06 | 2018-03-06 | A kind of preparation method of dictyophora phalloidea proteoglycan |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810182782.3A CN108250318A (en) | 2018-03-06 | 2018-03-06 | A kind of preparation method of dictyophora phalloidea proteoglycan |
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| CN108250318A true CN108250318A (en) | 2018-07-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868817A (en) * | 2021-02-04 | 2021-06-01 | 中南民族大学 | Euphausia superba oil edible product with protein as key |
-
2018
- 2018-03-06 CN CN201810182782.3A patent/CN108250318A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112868817A (en) * | 2021-02-04 | 2021-06-01 | 中南民族大学 | Euphausia superba oil edible product with protein as key |
| CN112868817B (en) * | 2021-02-04 | 2022-05-17 | 中南民族大学 | Euphausia superba oil edible product with protein as key |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180706 |
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| WW01 | Invention patent application withdrawn after publication |