CN111333747A - Preparation method of lentinan - Google Patents
Preparation method of lentinan Download PDFInfo
- Publication number
- CN111333747A CN111333747A CN201811551352.0A CN201811551352A CN111333747A CN 111333747 A CN111333747 A CN 111333747A CN 201811551352 A CN201811551352 A CN 201811551352A CN 111333747 A CN111333747 A CN 111333747A
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- China
- Prior art keywords
- lentinan
- extracting
- wall breaking
- collecting
- water
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a preparation method of lentinan, which comprises the following steps: cleaning Lentinus Edodes, removing impurities, and draining; crushing the shiitake mushrooms into superfine powder in a crusher, adding clear water, homogenizing, adding muramidase into the homogenate, and performing wall breaking treatment on shiitake mushroom cells; heating and extracting the mushroom cell homogenate after wall breaking, and then performing suction filtration to obtain supernatant; extracting for 3 times by the same method, collecting and mixing the filtrates after 3 times of extraction; adopting absolute ethyl alcohol to precipitate crude lentinus edodes polysaccharide in the extracting solution; dissolving Lentinus Edodes crude polysaccharide with distilled water, ultrafiltering with hollow fiber membrane, and collecting the filtrate; spray drying the filtrate to obtain lentinan. The invention effectively solves the defects of long extraction time and many impurities of a pure hot water extraction method, and the yield of the obtained lentinan is higher and can reach more than 4 percent; the lentinan extracted by the method has the advantages of molecular weight of 100-150 ten thousand, high purity, low impurity content and high pharmaceutical activity.
Description
Technical Field
The invention relates to the technical field of biological medicine preparation, in particular to a preparation method of lentinan.
Background
Lentinus Edodes, also known as Lentinus Edodes, XIANGXIN, Lentinus Edodes, and Balanophora Molina is fruiting body of Lentinus Edodes of Pleurotaceae. The mushroom is the second most edible mushroom in the world and is one of special products in China. The lentinula edodes essence is called as the king of delicacies from mountain, and is a high-protein low-fat nutritional health food. The shiitake mushroom is rich in vitamin B group, ferrum, potassium, provitamin D (converted into vitamin D after being sun-cured), sweet in taste and neutral in nature. It can be used for treating anorexia, hypoqi, and asthenia. Chinese medical scientists have well-known discussions of shiitake mushrooms. Modern medicine and nutriology are continuously and deeply researched, and the medicinal value of the shiitake is continuously explored. The content of ergosterol in the mushroom is very high, and the mushroom extract is effective in preventing and treating rickets.
Lentinan (β -1, 3-glucan) is one of the important chemical components of lentinan, can enhance the phagocytic capacity of abdominal macrophages, improves the anti-tumor efficacy of organisms, has no toxic or side effect, and is an ideal immune promoter.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a preparation method of lentinan.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a preparation method of lentinan comprises the following steps:
(1) pretreatment: cleaning a certain amount of shiitake mushrooms with clear water, removing impurities and draining;
(2) wall breaking treatment: crushing the shiitake mushrooms obtained in the step (1) into superfine powder in a crusher, adding clear water with the weight 5-10 times that of the shiitake mushrooms, homogenizing, then adding muramidase with the concentration of 1-2% (W/V), and performing wall breaking treatment on shiitake mushroom cells, wherein the enzymolysis temperature is 25-35 ℃, and the enzymolysis time is 1-2 hours;
(3) hot water leaching: heating the mushroom cell homogenate obtained in the step (2) after wall breaking to 75-90 ℃ in a water bath kettle, leaching for 60-180min, then adopting a vacuum pump to extract supernatant, and taking filter residue for later use;
(4) refluxing and re-extracting: extracting the filter residue obtained in the step (3) with 5-12 times of water under reflux for 2-3 times, each time for 60-180min, filtering, and collecting and combining the filtrates for later use;
(5) concentrating and precipitating with ethanol: adding absolute ethanol into the extracting solution obtained in the step (4) until the final concentration of the ethanol is 50-90% (v/v), and precipitating to obtain crude polysaccharide;
(6) and (3) ultrafiltration impurity removal: dissolving the crude polysaccharide obtained in the step (5) with 2-4 times of distilled water, performing ultrafiltration through a hollow fiber membrane, and collecting a permeate for later use;
(7) spray drying: and (4) spray drying the filtrate obtained in the step (6) to obtain the lentinan.
In a further technical scheme, the molecular weight cut-off of the hollow fiber membrane used in the step (6) is 100-150 ten thousand.
In a further technical scheme, the spray drying in the step (7) is carried out in a drying tower at the temperature range of: 75-105 ℃.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the method provided by the invention, the structure of the cell wall of the lentinus edodes is destroyed by combining mechanical homogenization with the wall-dissolving enzyme, so that the defects of long extraction time and many impurities in a pure hot water extraction method are effectively overcome, and the lentinan yield is higher and can reach more than 4%;
(2) the method of alcohol precipitation combined with ultrafiltration is adopted to remove impurities and pigments in the lentinan to the maximum extent so as to ensure the purity of the finished product, the molecular weight of the lentinan obtained by extraction is between 100 and 150 thousands, the purity is higher, and the impurity content is less.
Detailed Description
For a better understanding of the present invention, reference is made to the following examples. It is to be understood that these examples are for further illustration of the invention and are not intended to limit the scope of the invention. In addition, it should be understood that the invention is not limited to the above-described embodiments, but is capable of various modifications and changes within the scope of the invention.
Example 1
Cleaning a certain amount of shiitake mushrooms with clear water, removing impurities and draining; crushing the obtained lentinus edodes into superfine powder in a crusher, adding clear water with the weight 8 times that of the lentinus edodes, homogenizing, then adding muramidase with the concentration of 1.5% (W/V) to perform wall breaking treatment on lentinus edodes cells, wherein the enzymolysis temperature is 30 ℃, and the enzymolysis time is 2 hours; heating the obtained mushroom cell homogenate with wall broken to 82 deg.C in water bath, leaching for 120min, filtering with vacuum pump to obtain supernatant, and collecting residue; extracting under reflux for 3 times (120 min each time) by the same method, filtering, and collecting and mixing filtrates; adding anhydrous ethanol into the obtained extractive solution until ethanol concentration is 75% (v/v), and precipitating to obtain crude polysaccharide; dissolving the obtained crude polysaccharide with 3 times of distilled water, ultrafiltering with hollow fiber membrane with molecular weight cutoff of 125 ten thousand, and collecting the permeate; spray drying the obtained filtrate in a drying tower at 95 ℃ to obtain the lentinan.
As described above, the present invention can be preferably implemented.
Claims (3)
1. The preparation method of lentinan is characterized by comprising the following steps:
(1) pretreatment: cleaning a certain amount of shiitake mushrooms with clear water, removing impurities and draining;
(2) wall breaking treatment: crushing the shiitake mushrooms obtained in the step (1) into superfine powder in a crusher, adding clear water with the weight 5-10 times that of the shiitake mushrooms, homogenizing, then adding muramidase with the concentration of 1-2% (W/V), and performing wall breaking treatment on shiitake mushroom cells, wherein the enzymolysis temperature is 25-35 ℃, and the enzymolysis time is 1-2 hours;
(3) hot water leaching: heating the mushroom cell homogenate obtained in the step (2) after wall breaking to 75-90 ℃ in a water bath kettle, leaching for 60-180min, then adopting a vacuum pump to extract supernatant, and taking filter residue for later use;
(4) refluxing and re-extracting: extracting the filter residue obtained in the step (3) with 5-12 times of water under reflux for 2-3 times, each time for 60-180min, filtering, and collecting and combining the filtrates for later use;
(5) concentrating and precipitating with ethanol: adding absolute ethanol into the extracting solution obtained in the step (4) until the final concentration of the ethanol is 50-90% (v/v), and precipitating to obtain crude polysaccharide;
(6) and (3) ultrafiltration impurity removal: dissolving the crude polysaccharide obtained in the step (5) with 2-4 times of distilled water, performing ultrafiltration through a hollow fiber membrane, and collecting a permeate for later use;
(7) spray drying: and (4) spray drying the filtrate obtained in the step (6) to obtain the lentinan.
2. The method for preparing lentinan according to claim 1, wherein the molecular weight cut-off of the hollow fiber membrane used in step (6) is 100-150 ten thousand.
3. The method for preparing lentinan according to claim 1, wherein the spray drying in the step (7) is performed in a drying tower at a temperature ranging from: 75-105 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811551352.0A CN111333747A (en) | 2018-12-19 | 2018-12-19 | Preparation method of lentinan |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811551352.0A CN111333747A (en) | 2018-12-19 | 2018-12-19 | Preparation method of lentinan |
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| CN111333747A true CN111333747A (en) | 2020-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811551352.0A Pending CN111333747A (en) | 2018-12-19 | 2018-12-19 | Preparation method of lentinan |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112321739A (en) * | 2020-11-05 | 2021-02-05 | 海林市千菌方科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN114467630A (en) * | 2022-03-04 | 2022-05-13 | 南京师范大学 | Application of rice straw in culturing Coprinus cinereus |
-
2018
- 2018-12-19 CN CN201811551352.0A patent/CN111333747A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112321739A (en) * | 2020-11-05 | 2021-02-05 | 海林市千菌方科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN114467630A (en) * | 2022-03-04 | 2022-05-13 | 南京师范大学 | Application of rice straw in culturing Coprinus cinereus |
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Application publication date: 20200626 |
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| WD01 | Invention patent application deemed withdrawn after publication |