CN108220184A - Compound bacteria, preparation method and applications - Google Patents
Compound bacteria, preparation method and applications Download PDFInfo
- Publication number
- CN108220184A CN108220184A CN201711350263.5A CN201711350263A CN108220184A CN 108220184 A CN108220184 A CN 108220184A CN 201711350263 A CN201711350263 A CN 201711350263A CN 108220184 A CN108220184 A CN 108220184A
- Authority
- CN
- China
- Prior art keywords
- parts
- potassium
- compound bacteria
- weight
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention discloses a kind of compound bacteria, preparation method and applications, belong to fertilizer preparing technical field.The compound bacteria, as unit of parts by weight, including following raw material：20 32 parts of bacillus licheniformis, 12 22 parts of nitrobacteria, 10 15 parts of saccharomycete, 36 parts of photosynthetic bacteria, 6 10 parts of blown-ball Azotobacter, 49 parts of phosphobacteria, 35 parts of B. mucilaginocus, the compound bacteria be through overactivation, expand culture, centrifuge water removal and etc. made of, the compound bacteria of the present invention is applied to prepare three red sweet shaddock soil amendment fertilizers, which can improve the per mu yield of three red sweet shaddocks.
Description
【Technical field】
The invention belongs to fertilizer preparing technical fields, and in particular to a kind of compound bacteria, preparation method and applications.
【Background technology】
Shaddock also known as pomelo, fragrant goldenrain tree, grapefruit, interior purple etc. are the ripening fruits of Citrus paradisi Macfadyen, originate in China Fujian,
The southern areas such as Jiangxi, Hunan, Guangdong, Guangxi, Zhejiang, Sichuan.Pomelo fragrant, sour-sweet, Liang Run, full of nutrition, medical value
It is very high, it is one of fruit of people's eating and the fruit of most food therapy effect that medical field is generally acknowledged.Citron tea and pomelo peel
It all has practical value, and can be used as medicine.
The policy of China's modern times fruits industry development is " lower beach of going up a hill ", this just determines that most grapefruits are planted in
On mountain area, hills, sandy beach and salt-soda soil.The general soil property in these places is more barren, and dysplasia, the content of organic matter is low, unfavorable
In the growth and development of grapefruit.Particularly South China Tropical or subtropical zone, high temperature and humidity, the soil organism in shaddock garden
Content is few, grogs is thin, dysplasia, and mostly in acidity, iron aluminium is opposite to be gathered, and available phosphorus is less.Soil is in the pasty state, arid after rain
When soil block become especially hard, be unfavorable for the growth and development of shaddock.
Soil is one of important ecological environmental condition of grapefruit and builds high standard shaddock garden, ensures that shaddock garden is excellent
The material base of matter.Loam is the important link for realizing production modernization shaddock Orchard Soil management, its growth with grapefruit
Development is closely related with the taste quality of shaddock.Fully to develop and utilize soil resource, high-quality shaddock garden is established, creates shaddock
The good ecological environment of growth is set, extends the grapefruit economic life, soil improvement must be carried out by increasing economic efficiency.
A kind of Chinese patent application document " shaddock Orchard Soil modifying agent and preparation method thereof (application publication number：
CN106116952A) " open a kind of shaddock Orchard Soil modifying agent and preparation method thereof of knowing clearly, including following component：Organic fertilizer 18-
39 parts by weight, peat soil 5-12 parts by weight, montmorillonite 1-5 parts by weight, zeolite 1-5 parts by weight, sepiolite 2-6 parts by weight, calcium and magnesium
Phosphate fertilizer 4-8 parts by weight, bacillus licheniformis 1-3 parts by weight, blown-ball Azotobacter 1-3 parts by weight, actinobacillus capsulatus 1-3 weight
Part.Compared with prior art, the present invention is with organic fertilizer, peat soil, montmorillonite, zeolite, sepiolite, calcium magnesium phosphate, lichens gemma
Bacillus, blown-ball Azotobacter, actinobacillus capsulatus are prepared for shaddock Orchard Soil modifying agent for raw material, improve the soil in shaddock garden,
Supplemented with trace element, soil physics structure is improved, enhances soil fertility, however there is using after the soil amendment fertilizer
The problem of yield of three red sweet shaddocks obtained is relatively low.
【Invention content】
The present invention provides a kind of compound bacteria, preparation method and applications, to solve in a kind of Chinese patent application document " shaddock
Sub- Orchard Soil modifying agent and preparation method thereof (application publication number：CN106116952A soil improvement agent prescription basis disclosed in) "
On, how to optimize component, dosage etc., improve the per mu yield of three red sweet shaddocks.
For solution more than technical problem, the present invention uses following technical scheme：
A kind of compound bacteria, as unit of parts by weight, including following raw material：20-32 parts of bacillus licheniformis, nitrobacteria
12-22 parts, 10-15 parts of saccharomycete, 3-6 parts of photosynthetic bacteria, 6-10 parts of blown-ball Azotobacter, 4-9 parts of phosphobacteria, 3-5 parts of B. mucilaginocus.
Further, the compound bacteria, as unit of parts by weight, including following raw material：26 parts of bacillus licheniformis, nitre
Change 20 parts of bacterium, 12 parts of saccharomycete, 5 parts of photosynthetic bacteria, 8 parts of blown-ball Azotobacter, 8 parts of phosphobacteria, 3 parts of B. mucilaginocus.
The present invention also provides a kind of preparation methods of compound bacteria, include the following steps：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus
Ratio is activated at being 23-26 DEG C in temperature, expands culture by weight, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, brown ball fixed nitrogen will be expanded to step a
Bacterium, phosphobacteria, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 500-800 part water and be uniformly mixed, in temperature
It is after cultivating 15-18h at 24-26 DEG C to spend, and centrifuges water removal in the case where rotating speed is 2000-3000r/min, compound bacteria is made.
Further, the biological nutritive agent is as unit of parts by weight, including following raw material：50-60 parts of yeast extract, sucrose
30-50 parts, 14-22 parts of peptone, 15-20 parts of potassium acetate, 12-16 parts of sodium phosphate.
Further, the biological nutritive agent is as unit of parts by weight, including following raw material：54 parts of yeast extract, sucrose 42
Part, 18 parts of peptone, 16 parts of potassium acetate, 15 parts of sodium phosphate
Further, the biological growth stimulant is as unit of parts by weight, including following raw material：42-56 parts of cerium chloride,
25-30 parts of aluminum sulfate.
Further, the biological growth stimulant is as unit of parts by weight, including following raw material：52 parts of cerium chloride, sulphur
Sour 28 parts of aluminium.
The present invention also provides a kind of application of compound bacteria in three red sweet shaddock soil amendment fertilizers are prepared, the soil
Fertilizer is improved, including following raw material：Ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite powder, zeolite powder, sea bubble powder, calcium
Magnesium-phosphorus fertilizer, compound bacteria, diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch graft acrylic acid potassium；
The diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch graft acrylic acid potassium weight
Amount is than being (6-18):(3-6):(5-9):(2-4):(2.6-3.5):(2-4.6).
Further, the diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch-grafted third
The weight ratio of olefin(e) acid potassium is 15:5:8:3:3.2:3.8.
Further, the soil amendment fertilizer, as unit of parts by weight, including following raw material：52 parts of ammonium carbonate, phosphorus
Sour 13 parts of monoammonium, 24 parts of potassium chloride, 12 parts of peat soil, 4 parts of montmorillonite powder, 3 parts of zeolite powder, sea 7 parts of powder of bubble, calcium magnesium phosphate 10
Part, 26 parts of compound bacteria, 15 parts of diatomite, 5 parts of itaconic acid, 8 parts of polyvinyl alcohol, 3 parts of polyaminoacid potassium, 3.2 parts of polyethylene glycol oxide,
3.8 parts of starch graft acrylic acid potassium.
The invention has the advantages that：
(1) by the data of embodiment 1-3 and comparative example 8 as it can be seen that three red sweet shaddocks using embodiment 1-3 soil amendment fertilizers
Per mu yield significantly improves；Simultaneously by the data of embodiment 1-3 as it can be seen that embodiment 1 is optimum embodiment.
(2) by embodiment 1 and the data of comparative example 1-7 as it can be seen that diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium,
Polyethylene glycol oxide, starch graft acrylic acid potassium play synergistic effect in soil amendment fertilizer is prepared, and it is red that collaboration improves three
The per mu yield of sweet shaddock, this may be：Using diatomite as major ingredient, using itaconic acid as crosslinking agent, polyvinyl alcohol is a kind of water
Soluble high polymer has good film forming.Polyaminoacid potassium can increase soil porosity and water-holding capacity, help to improve soil
Earth retain water and nutrients ability creates good conditions for three red sweet shaddock root growth and developments.Polyethylene glycol oxide contains water-wet side, can be with answering
It closes fertiliser granulates to combine, polyethylene glycol oxide has good water resistance, and its strand can stretch well in water,
Even is arranged in interface, increases steric hindrance, makes the sub- rapid dispersion of compound fertilizer granule in water；Starch graft acrylic acid potassium can
As the slow releasing carrier material of fertilizer, when being used cooperatively with polyaminoacid potassium, fertilizer absorptivity can be increased.Diatomite is porous material
Material, while a large amount of hydroxyl is contained on its surface, can utilize the hydroxyl on diatomite surface, under itaconic acid modifying function, with answering
Close fertiliser granulates combine it is even closer, and under the filming function and starch graft acrylic acid potassium slow releasing function of polyvinyl alcohol,
Effective sustained release to compound fertilizer granule can be achieved so that compound fertilizer granule can slowly enter soil after entering in soil
In, slowly supply fertilizer particle is carried out to soil, soil depletion and hardened is avoided, in combination with the water conservation of polyaminoacid potassium
Fertilizer-preserving ability can effectively improve the efficiency and yield of three red sweet shaddock growths.
(3) diatomite in the present invention, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch-grafted third
Olefin(e) acid potassium is as reinforcement system, and embodiment 1-3 is by controlling diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyoxygenated
Ethylene, starch graft acrylic acid potassium weight ratio be (6-18):(3-6):(5-9):(2-4):(2.6-3.5):(2-4.6), it is real
It is used as the leading role raw material of reinforcement system in present reinforcement system using diatomite, polyethylene glycol oxide, while utilization itaconic acid
Crosslinking agent performance, the filming function of polyvinyl alcohol, polyaminoacid potassium can increase soil porosity and water-holding capacity, improve soil and protect
The ability of water fertilizer conservation and the slow releasing function of starch graft acrylic acid potassium so that entire reinforcement system applies to the soil of the present invention
The per mu yield of three red sweet shaddocks can be effectively improved in earth improvement fertilizer.
【Specific embodiment】
It for ease of more fully understanding the present invention, is illustrated by following instance, these examples belong to the protection of the present invention
Range, but do not limit the scope of the invention.
In embodiment, the soil amendment fertilizer, as unit of parts by weight, including following raw material：Ammonium carbonate 42-60
Part, 12-15 parts of monoammonium phosphate, 14-35 parts of potassium chloride, 8-15 parts of peat soil, 3-5 parts of montmorillonite powder, 2-4 parts of zeolite powder, Hai Pao
4-8 parts of powder, 7-12 parts of calcium magnesium phosphate, 20-30 parts of compound bacteria, 6-18 parts of diatomite, 3-6 parts of itaconic acid, 5-9 parts of polyvinyl alcohol,
2-4 parts of polyaminoacid potassium, 2.6-3.5 parts of polyethylene glycol oxide, 2-4.6 parts of starch graft acrylic acid potassium；
The compound bacteria is as unit of parts by weight, including following raw material：20-32 parts of bacillus licheniformis, nitrobacteria
12-22 parts, 10-15 parts of saccharomycete, 3-6 parts of photosynthetic bacteria, 6-10 parts of blown-ball Azotobacter, 4-9 parts of phosphobacteria, 3-5 parts of B. mucilaginocus；
The preparation method of the soil amendment fertilizer, includes the following steps：
S1：By ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite powder, zeolite powder, sea bubble powder, calcium magnesium phosphate, answer
It closes bacterium ratio by weight to mix, ferments at being 35-52 DEG C in temperature, ferment 5-10 days, fermentate is made；
The preparation method of compound bacteria, includes the following steps described in step S1：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus
Ratio is activated at being 23-26 DEG C in temperature, expands culture by weight, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, brown ball fixed nitrogen will be expanded to step a
Bacterium, phosphobacteria, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 500-800 part water and be uniformly mixed, in temperature
It is after cultivating 15-18h at 24-26 DEG C to spend, and centrifuges water removal in the case where rotating speed is 2000-3000r/min, compound bacteria is made；Institute
Biological nutritive agent is stated as unit of parts by weight, including following raw material：50-60 parts of yeast extract, 30-50 parts of sucrose, peptone 14-22
Part, 15-20 parts of potassium acetate, 12-16 parts of sodium phosphate；The biological growth stimulant is as unit of parts by weight, including following raw material：
42-56 parts of cerium chloride, 25-30 parts of aluminum sulfate；
S2：Fermentate made from step S1 at 50-62 DEG C is concentrated, obtains concentrate, by the concentrate in 65-72
It dries to water content≤4% at DEG C, is then granulated, compound fertilizer granules are made；
S3：Itaconic acid, polyvinyl alcohol, polyaminoacid potassium are added in reaction kettle by weight, stirs evenly, is heated to
72-80 DEG C, continue stirring l.2-2.5h, treat that polyvinyl alcohol is completely dissolved, stop heating, continue to stir, add in the silicon of parts by weight
Diatomaceous earth, polyethylene glycol oxide, starch graft acrylic acid potassium are warming up to 80-90 DEG C, accelerate stirring 1.2-1.8h, stop heating, continue
Stirring, is cooled to room temperature, and coating feed liquid is made；
S4：Compound fertilizer granules made from step S2 are put into the rotary drum of rotation, by coating feed liquid liter made from step S3
Then temperature is equably sprayed on compound fertilizer granules surface to 52-70 DEG C with high-pressure spray-painted rifle, then air drying to water content≤
1%, soil amendment fertilizer is made.
Below by more specific embodiment, the present invention will be described.
Embodiment 1
A kind of soil amendment fertilizer, as unit of parts by weight, including following raw material：52 parts of ammonium carbonate, 13 parts of monoammonium phosphate,
24 parts of potassium chloride, 12 parts of peat soil, 4 parts of montmorillonite powder, 3 parts of zeolite powder, sea 7 parts of powder of bubble, 10 parts of calcium magnesium phosphate, compound bacteria 26
Part, 15 parts of diatomite, 5 parts of itaconic acid, 8 parts of polyvinyl alcohol, 3 parts of polyaminoacid potassium, 3.2 parts of polyethylene glycol oxide, starch-grafted third
3.8 parts of olefin(e) acid potassium；
The compound bacteria is as unit of parts by weight, including following raw material：26 parts of bacillus licheniformis, nitrobacteria 20
Part, 12 parts of saccharomycete, 5 parts of photosynthetic bacteria, 8 parts of blown-ball Azotobacter, 8 parts of phosphobacteria, 3 parts of B. mucilaginocus；
The preparation method of the soil amendment fertilizer, includes the following steps：
S1：By ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite powder, zeolite powder, sea bubble powder, calcium magnesium phosphate, answer
It closes bacterium ratio by weight to mix, ferments at being 46 DEG C in temperature, ferment 8 days, fermentate is made；
The preparation method of compound bacteria, includes the following steps described in step S1：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus
Ratio is activated at being 23-26 DEG C in temperature, expands culture by weight, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, brown ball fixed nitrogen will be expanded to step a
Bacterium, phosphobacteria, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 700 parts of water are uniformly mixed, and are in temperature
After cultivating 17h at 25 DEG C, water removal is centrifuged in the case where rotating speed is 3000r/min, compound bacteria is made；The biological nutritive agent is with weight
Amount part is unit, including following raw material：54 parts of yeast extract, 42 parts of sucrose, 18 parts of peptone, 16 parts of potassium acetate, 15 parts of sodium phosphate；
The biological growth stimulant is as unit of parts by weight, including following raw material：52 parts of cerium chloride, 28 parts of aluminum sulfate；
S2：Fermentate made from step S1 at 60 DEG C is concentrated, obtains concentrate, the concentrate is done at 38 DEG C
It is dry to water content be 4%, be then granulated, be made compound fertilizer granules；
S3：Itaconic acid, polyvinyl alcohol, polyaminoacid potassium are added in reaction kettle by weight, stirs evenly, is heated to 75
DEG C, continue to stir 2h, treat that polyvinyl alcohol is completely dissolved, stop heating, continue to stir, add in diatomite, the polyoxygenated of parts by weight
Ethylene, starch graft acrylic acid potassium are warming up to 86 DEG C, accelerate stirring 1.5h, stop heating, continue to stir, be cooled to room temperature, make
Obtain coating feed liquid；
S4：Compound fertilizer granules made from step S2 are put into the rotary drum of rotation, by coating feed liquid liter made from step S3
Then temperature is equably sprayed on compound fertilizer granules surface to 65 DEG C with high-pressure spray-painted rifle, then air drying to water content is
1%, soil amendment fertilizer is made.
Embodiment 2
A kind of soil amendment fertilizer, as unit of parts by weight, including following raw material：43 parts of ammonium carbonate, 12 parts of monoammonium phosphate,
16 parts of potassium chloride, 8 parts of peat soil, 3 parts of montmorillonite powder, 2 parts of zeolite powder, sea bubble 5 parts of powder, 7 parts of calcium magnesium phosphate, 22 parts of compound bacteria,
6 parts of diatomite, 3 parts of itaconic acid, 5 parts of polyvinyl alcohol, 2 parts of polyaminoacid potassium, 3 parts of polyethylene glycol oxide, starch graft acrylic acid potassium
2.2 part；
The compound bacteria is as unit of parts by weight, including following raw material：20 parts of bacillus licheniformis, nitrobacteria 12
Part, 10 parts of saccharomycete, 3 parts of photosynthetic bacteria, 6 parts of blown-ball Azotobacter, 4 parts of phosphobacteria, 3 parts of B. mucilaginocus；
The preparation method of the soil amendment fertilizer, includes the following steps：
S1：By ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite powder, zeolite powder, sea bubble powder, calcium magnesium phosphate, answer
It closes bacterium ratio by weight to mix, ferments at being 35 DEG C in temperature, ferment 10 days, fermentate is made；
The preparation method of compound bacteria, includes the following steps described in step S1：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus
Ratio is activated at being 23 DEG C in temperature, expands culture by weight, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, brown ball fixed nitrogen will be expanded to step a
Bacterium, phosphobacteria, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 500 parts of water are uniformly mixed, and are in temperature
After cultivating 18h at 24 DEG C, water removal is centrifuged in the case where rotating speed is 2000r/min, compound bacteria is made；The biological nutritive agent is with weight
Amount part is unit, including following raw material：50 parts of yeast extract, 30 parts of sucrose, 14 parts of peptone, 15 parts of potassium acetate, 12 parts of sodium phosphate；
The biological growth stimulant is as unit of parts by weight, including following raw material：42 parts of cerium chloride, 26 parts of aluminum sulfate；
S2：Fermentate made from step S1 at 50 DEG C is concentrated, obtains concentrate, the concentrate is done at 65 DEG C
It is dry to water content be 3.6%, be then granulated, be made compound fertilizer granules；
S3：Itaconic acid, polyvinyl alcohol, polyaminoacid potassium are added in reaction kettle by weight, stirs evenly, is heated to 72
DEG C, continue to stir 2.5h, treat that polyvinyl alcohol is completely dissolved, stop heating, continue to stir, add in diatomite, the polyoxy of parts by weight
Change ethylene, starch graft acrylic acid potassium, be warming up to 80 DEG C, accelerate stirring 1.8h, stop heating, continue to stir, be cooled to room temperature,
Coating feed liquid is made；
S4：Compound fertilizer granules made from step S2 are put into the rotary drum of rotation, by coating feed liquid liter made from step S3
Then temperature is equably sprayed on compound fertilizer granules surface to 52 DEG C with high-pressure spray-painted rifle, then air drying to water content is
0.8%, soil amendment fertilizer is made.
Embodiment 3
A kind of soil amendment fertilizer, as unit of parts by weight, including following raw material：58 parts of ammonium carbonate, 15 parts of monoammonium phosphate,
32 parts of potassium chloride, 15 parts of peat soil, 5 parts of montmorillonite powder, 4 parts of zeolite powder, sea 8 parts of powder of bubble, 11 parts of calcium magnesium phosphate, compound bacteria 28
Part, 17 parts of diatomite, 6 parts of itaconic acid, 9 parts of polyvinyl alcohol, 4 parts of polyaminoacid potassium, 3.5 parts of polyethylene glycol oxide, starch-grafted third
4.3 parts of olefin(e) acid potassium；
The compound bacteria is as unit of parts by weight, including following raw material：32 parts of bacillus licheniformis, nitrobacteria 22
Part, 15 parts of saccharomycete, 6 parts of photosynthetic bacteria, 10 parts of blown-ball Azotobacter, 9 parts of phosphobacteria, 5 parts of B. mucilaginocus；
The preparation method of the soil amendment fertilizer, includes the following steps：
S1：By ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite powder, zeolite powder, sea bubble powder, calcium magnesium phosphate, answer
It closes bacterium ratio by weight to mix, ferments at being 52 DEG C in temperature, ferment 5 days, fermentate is made；
The preparation method of compound bacteria, includes the following steps described in step S1：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus
Ratio is activated at being 26 DEG C in temperature, expands culture by weight, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, brown ball fixed nitrogen will be expanded to step a
Bacterium, phosphobacteria, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 800 parts of water are uniformly mixed, and are in temperature
After cultivating 15h at 26 DEG C, water removal is centrifuged in the case where rotating speed is 3000r/min, compound bacteria is made；The biological nutritive agent is with weight
Amount part is unit, including following raw material：60 parts of yeast extract, 50 parts of sucrose, 22 parts of peptone, 20 parts of potassium acetate, 16 parts of sodium phosphate；
The biological growth stimulant is as unit of parts by weight, including following raw material：56 parts of cerium chloride, 30 parts of aluminum sulfate；
S2：Fermentate made from step S1 at 62 DEG C is concentrated, obtains concentrate, the concentrate is done at 72 DEG C
It is dry to water content be 3%, be then granulated, be made compound fertilizer granules；
S3：Itaconic acid, polyvinyl alcohol, polyaminoacid potassium are added in reaction kettle by weight, stirs evenly, is heated to 80
DEG C, continue stirring l.2h, treat that polyvinyl alcohol is completely dissolved, stop heating, continue to stir, add in diatomite, the polyoxy of parts by weight
Change ethylene, starch graft acrylic acid potassium, be warming up to 90 DEG C, accelerate stirring 1.2h, stop heating, continue to stir, be cooled to room temperature,
Coating feed liquid is made；
S4：Compound fertilizer granules made from step S2 are put into the rotary drum of rotation, by coating feed liquid liter made from step S3
Then temperature is equably sprayed on compound fertilizer granules surface to 70 DEG C with high-pressure spray-painted rifle, then air drying to water content is
0.7%, soil amendment fertilizer is made.
Comparative example 2
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Diatomite.
Comparative example 3
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Itaconic acid.
Comparative example 4
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Polyvinyl alcohol.
Comparative example 5
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Polyaminoacid potassium.
Comparative example 6
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Polyethylene glycol oxide.
Comparative example 7
It is essentially identical with the preparation process of embodiment 1, have only unlike prepare and lack in the raw material of soil amendment fertilizer
Starch graft acrylic acid potassium.
Comparative example 8
Using a kind of Chinese patent application document " shaddock Orchard Soil modifying agent and preparation method thereof (application publication number：
CN106116952A) " technique of embodiment 1-3 prepares soil conditioner.
Enforcement place plants identical product in Nanning Qingxiu District cleverness town, 13 pieces of basically identical plot of planning condition
Kind and the basically identical three red sweet shaddocks of specification, respectively using the soil amendment fertilizer in embodiment 1-3 and comparative example 1-8, wherein
The soil conditioner of embodiment 1-3 in comparative example 8 is separately tested, during application, by three red sweet shaddock shaddock Orchard Soil improving fertilizers
Material is ploughed under in soil when earth's surface, site preparation according to uniformly being spread fertilizer over the fields before 50 kilograms of amount of application site preparations per acre, is sufficiently mixed with soil, three is red
After sweet shaddock maturation, per mu yield is detected, testing result is as shown in the table.
| Test group | Per mu yield (kg) |
| Embodiment 1 | 2975.63 |
| Embodiment 2 | 2817.19 |
| Embodiment 3 | 2904.51 |
| Comparative example 1 | 2674.01 |
| Comparative example 2 | 2916.28 |
| Comparative example 3 | 2948.29 |
| Comparative example 4 | 2955.25 |
| Comparative example 5 | 2937.17 |
| Comparative example 6 | 2926.25 |
| Comparative example 7 | 2941.86 |
| Comparative example 8 | 2512.64-2601.82 |
As seen from the above table：(1) by the data of embodiment 1-3 and comparative example 8 as it can be seen that using embodiment 1-3 soil improvements fertilizer
Three red sweet shaddock per mu yields of material significantly improve；Simultaneously by the data of embodiment 1-3 as it can be seen that embodiment 1 is optimal implementation
Example..
(2) by embodiment 1 and the data of comparative example 1-7 as it can be seen that diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium,
Polyethylene glycol oxide, starch graft acrylic acid potassium play synergistic effect in soil amendment fertilizer is prepared, and it is red that collaboration improves three
The per mu yield of sweet shaddock, this may be：Using diatomite as major ingredient, using itaconic acid as crosslinking agent, polyvinyl alcohol is a kind of water
Soluble high polymer has good film forming.Polyaminoacid potassium can increase soil porosity and water-holding capacity, help to improve soil
Earth retain water and nutrients ability creates good conditions for three red sweet shaddock root growth and developments.Polyethylene glycol oxide contains water-wet side, can be with answering
It closes fertiliser granulates to combine, polyethylene glycol oxide has good water resistance, and its strand can stretch well in water,
Even is arranged in interface, increases steric hindrance, makes the sub- rapid dispersion of compound fertilizer granule in water；Starch graft acrylic acid potassium can
As the slow releasing carrier material of fertilizer, when being used cooperatively with polyaminoacid potassium, fertilizer absorptivity can be increased.Diatomite is porous material
Material, while a large amount of hydroxyl is contained on its surface, can utilize the hydroxyl on diatomite surface, under itaconic acid modifying function, with answering
Close fertiliser granulates combine it is even closer, and under the filming function and starch graft acrylic acid potassium slow releasing function of polyvinyl alcohol,
Effective sustained release to compound fertilizer granule can be achieved so that compound fertilizer granule can slowly enter soil after entering in soil
In, slowly supply fertilizer particle is carried out to soil, soil depletion and hardened is avoided, in combination with the water conservation of polyaminoacid potassium
Fertilizer-preserving ability can effectively improve the efficiency and yield of three red sweet shaddock growths.
Diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch graft acrylic acid in the present invention
Potassium as reinforcement system, embodiment 1-3 by control diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide,
The weight ratio of starch graft acrylic acid potassium is (6-18):(3-6):(5-9):(2-4):(2.6-3.5):(2-4.6) is realized and is being mended
The leading role raw material of reinforcement system, while the crosslinking agent of utilization itaconic acid are used as in strong system using diatomite, polyethylene glycol oxide
Performance, the filming function of polyvinyl alcohol, polyaminoacid potassium can increase soil porosity and water-holding capacity, improve soil water-reataining fertilizer-keeping
Ability and starch graft acrylic acid potassium slow releasing function so that entire reinforcement system applies to the soil improvement of the present invention
The per mu yield of three red sweet shaddocks can be effectively improved in fertilizer.
More than content is it cannot be assumed that present invention specific implementation is confined to these explanations, for the technical field of the invention
Those of ordinary skill for, under the premise of present inventive concept is not departed from, several simple deduction or replace can also be made, all should
When being considered as belonging to present invention scope of patent protection determined by the appended claims.
Claims (10)
1. a kind of compound bacteria, which is characterized in that as unit of parts by weight, including following raw material：20-32 parts of bacillus licheniformis,
12-22 parts of nitrobacteria, 10-15 parts of saccharomycete, 3-6 parts of photosynthetic bacteria, 6-10 parts of blown-ball Azotobacter, 4-9 parts of phosphobacteria, B. mucilaginocus
3-5 parts.
2. compound bacteria according to claim 1, which is characterized in that as unit of parts by weight, including following raw material：Lichens bud
26 parts of spore bacillus, 20 parts of nitrobacteria, 12 parts of saccharomycete, 5 parts of photosynthetic bacteria, 8 parts of blown-ball Azotobacter, 8 parts of phosphobacteria, B. mucilaginocus 3
Part.
3. a kind of preparation method of compound bacteria according to claim 1 or 2, which is characterized in that include the following steps：
A, respectively by bacillus licheniformis, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphobacteria, B. mucilaginocus by weight
Amount part ratio is activated at being 23-26 DEG C in temperature, expands culture, with spare；
B, the bacillus licheniformis after culture, nitrobacteria, saccharomycete, photosynthetic bacteria, blown-ball Azotobacter, phosphorus will be expanded to step a
Bacterium, B. mucilaginocus mix and add biological growth stimulant, biological nutritive agent, 500-800 part water and be uniformly mixed, and are in temperature
After cultivating 15-18h at 24-26 DEG C, water removal is centrifuged in the case where rotating speed is 2000-3000r/min, compound bacteria is made.
4. the preparation method of compound bacteria according to claim 3, which is characterized in that the biological nutritive agent using parts by weight as
Unit, including following raw material：50-60 parts of yeast extract, 30-50 parts of sucrose, 14-22 parts of peptone, 15-20 parts of potassium acetate, phosphoric acid
12-16 parts of sodium.
5. the preparation method of compound bacteria according to claim 4, which is characterized in that the biological nutritive agent using parts by weight as
Unit, including following raw material：54 parts of yeast extract, 42 parts of sucrose, 18 parts of peptone, 16 parts of potassium acetate, 15 parts of sodium phosphate.
6. the preparation method of compound bacteria according to claim 3, which is characterized in that the biological growth stimulant is with weight
Part is unit, including following raw material：42-56 parts of cerium chloride, 25-30 parts of aluminum sulfate.
7. the preparation method of compound bacteria according to claim 6, which is characterized in that the biological growth stimulant is with weight
Part is unit, including following raw material：52 parts of cerium chloride, 28 parts of aluminum sulfate.
8. a kind of application of compound bacteria according to claim 1 or 2 in three red sweet shaddock soil amendment fertilizers are prepared,
It is characterized in that, the soil amendment fertilizer, including following raw material：Ammonium carbonate, monoammonium phosphate, potassium chloride, peat soil, montmorillonite
Powder, zeolite powder, sea bubble powder, calcium magnesium phosphate, compound bacteria, diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyoxyethylene
Alkene, starch graft acrylic acid potassium；
The diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch graft acrylic acid potassium weight ratio
For (6-18):(3-6):(5-9):(2-4):(2.6-3.5):(2-4.6).
9. application of the compound bacteria according to claim 8 in three red sweet shaddock soil amendment fertilizers are prepared, feature exist
In, the diatomite, itaconic acid, polyvinyl alcohol, polyaminoacid potassium, polyethylene glycol oxide, starch graft acrylic acid potassium weight ratio
It is 15:5:8:3:3.2:3.8.
10. application of the compound bacteria according to claim 1 in three red sweet shaddock soil amendment fertilizers are prepared, feature exist
In the soil amendment fertilizer, as unit of parts by weight, including following raw material：52 parts of ammonium carbonate, 13 parts of monoammonium phosphate, chlorine
Change 24 parts of potassium, 12 parts of peat soil, 4 parts of montmorillonite powder, 3 parts of zeolite powder, sea bubble 7 parts of powder, 10 parts of calcium magnesium phosphate, 26 parts of compound bacteria,
15 parts of diatomite, 5 parts of itaconic acid, 8 parts of polyvinyl alcohol, 3 parts of polyaminoacid potassium, 3.2 parts of polyethylene glycol oxide, starch graft acrylic acid
3.8 parts of potassium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711350263.5A CN108220184A (en) | 2017-12-15 | 2017-12-15 | Compound bacteria, preparation method and applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711350263.5A CN108220184A (en) | 2017-12-15 | 2017-12-15 | Compound bacteria, preparation method and applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108220184A true CN108220184A (en) | 2018-06-29 |
Family
ID=62652271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711350263.5A Pending CN108220184A (en) | 2017-12-15 | 2017-12-15 | Compound bacteria, preparation method and applications |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108220184A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110590434A (en) * | 2019-10-24 | 2019-12-20 | 佳木斯旺伟生物能源科技有限公司 | Biological bacterium straw fermentation fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649655A (en) * | 2012-04-27 | 2012-08-29 | 上海中科高等研究院 | Carbon-based compound fertilizer containing probiotic organisms and preparation method of carbon-based compound fertilizer |
| CN106116952A (en) * | 2016-06-27 | 2016-11-16 | 莆田秀屿区群韬农业技术开发有限公司 | A kind of Fructus Citri grandis Orchard Soil modifying agent and preparation method thereof |
| CN106278563A (en) * | 2016-08-01 | 2017-01-04 | 韦波 | A kind of organic fertilizer |
| WO2017192117A1 (en) * | 2016-05-02 | 2017-11-09 | Advanced Biological Marketing, Inc. | Highly effective and multifunctional microbial compositions and uses |
-
2017
- 2017-12-15 CN CN201711350263.5A patent/CN108220184A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649655A (en) * | 2012-04-27 | 2012-08-29 | 上海中科高等研究院 | Carbon-based compound fertilizer containing probiotic organisms and preparation method of carbon-based compound fertilizer |
| WO2017192117A1 (en) * | 2016-05-02 | 2017-11-09 | Advanced Biological Marketing, Inc. | Highly effective and multifunctional microbial compositions and uses |
| CN106116952A (en) * | 2016-06-27 | 2016-11-16 | 莆田秀屿区群韬农业技术开发有限公司 | A kind of Fructus Citri grandis Orchard Soil modifying agent and preparation method thereof |
| CN106278563A (en) * | 2016-08-01 | 2017-01-04 | 韦波 | A kind of organic fertilizer |
Non-Patent Citations (1)
| Title |
|---|
| 林金新: "生物有机肥在琯溪蜜柚上的应用效果分析", 《台湾农业探索》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110590434A (en) * | 2019-10-24 | 2019-12-20 | 佳木斯旺伟生物能源科技有限公司 | Biological bacterium straw fermentation fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102329754B (en) | Novel strain of streptomyces fradiae and application thereof | |
| CN108083898A (en) | Soil conditioner, preparation method and applications | |
| CN104086317A (en) | Organic ecological fertilizer for grapes and preparation method thereof | |
| CN107759420A (en) | A kind of Composite microbial fertilizer for paddy | |
| CN109851454A (en) | Agricultural complex microorganism modifying agent of alkalinity and preparation method thereof | |
| CN106833664A (en) | Soil conditioner and preparation method thereof | |
| CN105037033A (en) | Nutritional additive for improving water and soil in seedling stage and preparation method thereof | |
| CN106008095A (en) | Microbe soil restoration agent and preparation method thereof | |
| CN106305304A (en) | Planting method of sand sugar oranges based on container nursery substrate | |
| CN108101628A (en) | The preparation process of three red sweet shaddock plantation soil amendment fertilizers | |
| CN108147919A (en) | Microorganism seaweed composite fertilizer and preparation method thereof | |
| CN112062634A (en) | Rice slow-release fertilizer and preparation and application methods thereof | |
| CN109111287A (en) | A kind of Water-solubility foliar fertilizer and preparation method thereof promoting crop growth | |
| CN107879816A (en) | Soil amendment fertilizer | |
| CN109336689A (en) | A kind of blueberry container culturing substrate | |
| CN102942409B (en) | Humic acid nutrient-gathering fertilizer and production method thereof | |
| CN108220184A (en) | Compound bacteria, preparation method and applications | |
| CN107721602A (en) | A kind of alkaline Sandy Soil improvement matrix and preparation method thereof | |
| CN104211496A (en) | Organic fertilizer with water retention suitable for cucumber plantation, and its preparation method | |
| CN109180373A (en) | A kind of pueraria lobata organic and inorganic compound fertilizer and preparation method thereof | |
| CN108101626A (en) | A kind of ecology sustained release organic film fertilizer and preparation method | |
| CN115010538A (en) | Two-component composite microorganism water-retention temperature-control slow-release fertilizer and preparation method and application thereof | |
| CN109627103B (en) | Water-soluble fertilizer for vegetable planting and preparation method and application thereof | |
| CN108069780A (en) | Soil conditioner and its application in the red sweet shaddock of plantation three | |
| CN109627104B (en) | Water-soluble fertilizer for fruit tree planting and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180629 |