CN108208685A - A kind of preparation method of fish oil soft capsule - Google Patents
A kind of preparation method of fish oil soft capsule Download PDFInfo
- Publication number
- CN108208685A CN108208685A CN201711363537.4A CN201711363537A CN108208685A CN 108208685 A CN108208685 A CN 108208685A CN 201711363537 A CN201711363537 A CN 201711363537A CN 108208685 A CN108208685 A CN 108208685A
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- Prior art keywords
- parts
- fish oil
- mixing
- add
- mass ratio
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- 239000007901 soft capsule Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
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- 241000972773 Aulopiformes Species 0.000 claims abstract description 31
- 235000019515 salmon Nutrition 0.000 claims abstract description 31
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000004332 deodorization Methods 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 239000006228 supernatant Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 14
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- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 10
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- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 10
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- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 2
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/276—Treatment with inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B5/00—Preserving by using additives, e.g. anti-oxidants
- C11B5/0092—Mixtures
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B7/00—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils
- C11B7/0083—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils with addition of auxiliary substances, e.g. cristallisation promotors, filter aids, melting point depressors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Wood Science & Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to health product technology fields, and in particular to a kind of preparation method of fish oil soft capsule.The present invention extracts the glyceride ingredient in the unrighted acid in salmon liver under the conditions of relatively mild using enzyme catalysis method, lipase first hydrolyzes the saturation on fish oil glyceride and low unrighted acid, expose hydroxyl, ester bond occurs with free EPA and DHA again and synthesizes the EPA and DHA that enrichment is obtained by the reaction, polyunsaturated fatty acid caused by avoiding high temperature polymerize, the problems such as oxidation and conjugation are with trans-ization, saturated fatty acid in ethyl ester fish oil is removed by way of urea clathrate in the process, using vacuum deodorization, remove the fishy smell in fish oil, increase palatability, safety, unharmful substance generates.
Description
Technical field
The present invention relates to health product technology fields, and in particular to a kind of preparation method of fish oil soft capsule.
Background technology
Fish oil is the general designation of oily substance in fish body, it includes body oil, liver oil and cerebrol, and main fish oil is a kind of from greasiness fish
The grease of class extraction, is polyunsaturated fatty acid rich in ω -3, main composition is DHA（Docosahexaenoic acid）And EPA
（Eicosapentaenoic acid）, for the total content of main component EPA and DHA generally more than 34%, DHA is a kind of special unsaturation
Aliphatic acid has the factor of activating brain cell, and EPA is equally a kind of unrighted acid, containing there are many be easy to sanguimotor
Protective factors, this kind of polyunsaturated fatty acid have prevention of arterial hardening, headstroke, hypertension, coronary heart disease and cardiopathic work(
Effect.
Fish oil soft capsule is mainly that the fish body from deep-sea extracts EPA, DHA to complete.In recent years, research hair
The omega-3 polyunsaturated fatty acids such as existing EPA and DHA have the function of to improve cerebral function especially have very big change to health
Kind effect, can adjust blood fat, reduce blood viscosity, prevent thrombosis, prevent cardiovascular and cerebrovascular disease；And blood can be passed through
Brain barrier, activating brain cell promote brain development, prevent senile dementia, improve eyesight, the decline for delaying eyesight.
Fish oil soft capsule type is various at present, is using fish oil, triglycerides, natural complex E oil, glycerine, bright mostly
Glue etc. is prepared, and remains the problem of certain in practical applications：
（1）There is strong fishlike smell, it is not high to eat enthusiasm.
（2）The fish oil of preparation is extremely sensitive to light, heat and oxygen, is easily aoxidized, so as to lump, ingredient the effect of in fish oil
DHA and EPA are ineffective, reduce the nutritive value of fish oil.
（3）Nutritional ingredient is relatively simple so that improve brain memory the effect of it is undesirable.
Therefore, we it is necessary to designs again to prepare a kind of novel fish oil soft capsule, nowadays city is solved with this
The problems of fish oil soft capsule on field, being preferably adapted to society has relevant department to obtain demand.
Invention content
The technical problems to be solved by the invention：For there is strong fishlike smell at present, edible enthusiasm is not high, preparation
Fish oil is extremely sensitive to light, heat and oxygen, is easily aoxidized, and so as to lump, components D HA and EPA are ineffective the effect of in fish oil, drop
The problem of low nutritive value of fish oil, provide a kind of preparation method of fish oil soft capsule.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of fish oil soft capsule, the preparation method include the following steps：
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8 ~ 8.5, and it is even to add in salmon liver
The phosphate of grind slurries quality 2 ~ 5%, is stirred, enzymolysis, in 90 ~ 95 DEG C of enzyme deactivation 5 ~ 15min, 8000 r/min centrifugations 10
Min takes supernatant A in mass ratio 1：8 add in 50 ~ 70 DEG C of distilled water, and oscillation shakes up, and 8000 r/min centrifuge 10 ~ 15 again
Min collects supernatant B；
（2）By supernatant B in mass ratio 10：4：0.2 ~ 0.3 adds in absolute ethyl alcohol and sodium hydroxide, reaction, and rotary evaporation takes rotation
Turn evaporant in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, and decolourize 2 ~ 3h, obtains decoloration object, takes de-
Color object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 10 ~ 12 h are kept in -5 DEG C, and filtering takes filtrate, is placed in de-
Smelly tower keeps 1 ~ 2h, obtains deodorization liquid, spare；
（3）Spine grape wine ground-slag end is taken to be passed through steam, powder in mass ratio 1 after steam blasting is collected in explosion：15 add in 80%
Ethanol solution, ultrasound, through 0.22 μm of organic filtering with microporous membrane, takes filtrate in mass ratio 5：1：2 add in tea polyphenols and vitamin E
Mixing, obtains mixing antioxidant；
（4）According to the mass fraction, 8 ~ 12 parts of soybean lecithins, 1 ~ 2 part of cholesterol, 3 ~ 4 parts of Tween-80s, 5 ~ 6 parts of steps are taken（2）
Spare deodorization liquid, 12 ~ 15 parts of mixing antioxidants, 60 ~ 70 parts of absolute ethyl alcohols, the PBS of 20 ~ 30 parts of a concentration of 0.05mol/L delay
Fliud flushing, oscillation 1 ~ 2h of mixing, rotary evaporation, ultrasound cross 0.2 μm of polycarbonate membrane, obtain suspension；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 12 ~ 15 parts of mixed liquor As, 8 ~ 14 parts of mixed liquid Bs, 20 ~ 30 parts
Suspension, 12 ~ 18 parts of composite nutrient mixing, stirring pour into mold, in 25 ~ 30 DEG C of dry 24 ~ 36h, obtain fish oil soft capsule.
The step（1）Middle salmon liver homogenate liquid is that salmon is taken out liver, washs, will wash in physiological saline
The salmon liver washed takes homogenate freezen protective in -3 ~ 4 DEG C of homogenate；Enzymatic hydrolysis condition is 40 ~ 45 DEG C, 300 r/min stirrings
Mixing digests 4 ~ 5 h.
The step（2）Middle reaction condition is 45 ~ 55 DEG C of 1.5 ~ 2h of reaction.
The step（3）Middle steam blasting condition：1.5MPa, 150 ~ 180s of steam blasting.
5. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（4）In surpass
Sound condition is：In -3 ~ -4 DEG C, 5 ~ 6min of 350W ultrasounds.
The step（5）Middle composite nutrient is：According to the mass fraction, 12 ~ 15 parts of propolis are taken, 4 ~ 5 parts of Radix Angelicae Sinensis, 3 ~ 6 parts
Aloe, 2 ~ 4 parts of matrimony vine mixing, grinding homogenate obtain composite nutrient.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention is extracted under the conditions of relatively mild using enzyme catalysis method in the unrighted acid in salmon liver
Glyceride ingredient, lipase first hydrolyzes the saturation on fish oil glyceride and low unrighted acid, exposes hydroxyl,
Ester bond occurs with free EPA and DHA again and synthesizes the EPA and DHA that enrichment is obtained by the reaction, avoids how unsaturated caused by high temperature
The problems such as aliphatic acid polymerization, oxidation and conjugation are with trans-ization, removes by way of urea clathrate in ethyl ester fish oil in the process
Saturated fatty acid using vacuum deodorization, removes the fishy smell in fish oil, increases palatability, safety, unharmful substance generation；
（2）Fish oil in the present invention is extremely sensitive to light, heat and oxygen, oxidizable decomposition, on the one hand extracts in Vitis davidii Foex schlempe
Active material, predominantly resveratrol is a kind of non-flavonoids polyphenolic substance, and resveratrol has lipid-antioxidant activity effect
It is because the phenolic hydroxyl group in its structure can effectively remove the free radical generated in oil peroxidation reaction as hydrogen donor, with it
Stable compound is combined into, so as to block the startup of oil peroxidation chain type cascade reaction, have prevents fish oil from aoxidizing well
Performance, compounded with tea polyphenols and vitamin E, there is anti-oxidant and health care synergistic effect, on the other hand by the effective of fish oil
For substance encapsulation in nano liposomes, the property with penetration cell, targeting enhances the performance of fish oil effect, can
It, can also controlled release and long-acting slow-release to embed hydrophilic and lipophilic functional components simultaneously；
（3）The present invention adds in auxiliary type nutriment, and propolis has expansion coronary vasodilator, reduces blood fat, anti-platelet aggregation
The effect of, Radix Angelicae Sinensis has the effect of benefiting blood and regulating blood circulation, strengthen immunity, and aloe has maintaining beauty and keeping young, and matrimony vine grows with nourishing the liver
Kidney moistening lung effect can enrich the nutritional ingredient of fish oil, and auxiliary fish oil is anti-oxidant and is preferably absorbed by human body；
（4）The present invention wraps up fish oil active material by soft capsule wall material, have pollute it is small, be not easy adhesion, do not occur
Cross-linking reaction, property are stablized, and enhance water retention property, extend the shelf-life, are not easy shrivelled, and dehydration is rotten.
Specific embodiment
Salmon liver homogenate liquid：Salmon is taken out into liver, is washed 3 ~ 5 times in physiological saline, three will washed
Literary fish liver takes homogenate freezen protective in -3 ~ 4 DEG C of homogenate.
Vitis davidii Foex schlempe：Purple autumn Vitis davidii Foex, Hunan Huachun Zhuangyuan Wines Co., Ltd..
Composite nutrient：According to the mass fraction, 12 ~ 15 parts of propolis are taken, 4 ~ 5 parts of Radix Angelicae Sinensis, 3 ~ 6 portions of aloes, 2 ~ 4 portions of matrimony vines mix
It closes, grinding homogenate obtains composite nutrient.
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8 ~ 8.5, add in salmon liver
The phosphate of dirty homogenate quality 2 ~ 5% is warming up to 40 ~ 45 DEG C, and 300 r/min are stirred, and digests 4 ~ 5 h, then at 90 ~
95 DEG C of enzyme deactivation 5 ~ 15min, 8000 r/min 10 min of centrifugation, take supernatant A in mass ratio 1：8 add in 50 ~ 70 DEG C of distilled water,
Oscillation shakes up, and 8000 r/min centrifuge 10 ~ 15 min again, collects supernatant B；
（2）By supernatant B in mass ratio 10：4：0.2 ~ 0.3 adds in absolute ethyl alcohol and sodium hydroxide, is warming up to 45 ~ 55 DEG C of reactions
1.5 ~ 2h, rotary evaporation take rotary evaporation object in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, take off
2 ~ 3h of color obtains decoloration object, takes decoloration object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 10 ~ 12 are kept in -5 DEG C
H, filtering, takes filtrate, is placed in deodorizing tower, keeps 1 ~ 2h, obtains deodorization liquid, spare；
（3）It takes spine grape wine ground-slag end in 1.5MPa, be passed through 150 ~ 180s of steam blasting, powder presses quality after collecting steam blasting
Than 1：15 add in 80% ethanol solution, in 55 ~ 60 DEG C of ultrasound 40min, through 0.22 μm of organic filtering with microporous membrane, take filtrate, press
Mass ratio 5：1：2 add in tea polyphenols and vitamin E mixing, obtain mixing antioxidant；
（4）According to the mass fraction, 8 ~ 12 parts of soybean lecithins, 1 ~ 2 part of cholesterol, 3 ~ 4 parts of Tween-80s, 5 ~ 6 parts of steps are taken（2）
Spare deodorization liquid, 12 ~ 15 parts of mixing antioxidants, 60 ~ 70 parts of absolute ethyl alcohols, the PBS of 20 ~ 30 parts of a concentration of 0.05mol/L delay
Fliud flushing, oscillation 1 ~ 2h of mixing, rotary evaporation in -3 ~ -4 DEG C, 350W 5 ~ 6min of ultrasound, are crossed 0.2 μm of polycarbonate membrane, are obtained suspended
Liquid；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 12 ~ 15 parts of mixed liquor As, 8 ~ 14 parts of mixed liquid Bs, 20 ~ 30 parts
Suspension, the mixing of 12 ~ 18 parts of composite nutrients, stir 1 ~ 2h in 85 ~ 90 DEG C, pour into mold, in 25 ~ 30 DEG C of dry 24 ~ 36h,
Obtain fish oil soft capsule.
Embodiment 1
Salmon liver homogenate liquid：Salmon is taken out into liver, is washed 3 times in physiological saline, the salmon liver that will have been washed
It is dirty to be homogenized in -3 DEG C, take homogenate freezen protective.
Vitis davidii Foex schlempe：Purple autumn Vitis davidii Foex, Hunan Huachun Zhuangyuan Wines Co., Ltd..
Composite nutrient：According to the mass fraction, 12 parts of propolis are taken, 4 parts of Radix Angelicae Sinensis, 3 portions of aloes, 2 parts of matrimony vines mixing, grinding is even
Slurry, obtains composite nutrient.
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8, and it is even to add in salmon liver
The phosphate of grind slurries quality 2% is warming up to 40 DEG C, and 300 r/min are stirred, and digests 4h, then at 90 DEG C of enzyme deactivation 5min,
8000 r/min centrifuge 10 min, take supernatant A in mass ratio 1：8 add in 50 DEG C of distilled water, and oscillation shakes up, 8000 r/min
10 min are centrifuged again, collect supernatant B；
（2）By supernatant B in mass ratio 10：4：0.2 adds in absolute ethyl alcohol and sodium hydroxide, is warming up to 45 DEG C of reaction 1.5h, rotation
Turn evaporation, take rotary evaporation object in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, and decolourize 23h, obtains
Decolourize object, takes decoloration object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 10 h are kept in -5 DEG C, and filtering takes filter
Liquid is placed in deodorizing tower, keeps 1h, obtains deodorization liquid, spare；
（3）It takes spine grape wine ground-slag end in 1.5MPa, be passed through steam blasting 150s, collect powder in mass ratio 1 after steam blasting：
15 add in 80% ethanol solution, in 55 DEG C of ultrasound 40min, through 0.22 μm of organic filtering with microporous membrane, take filtrate, in mass ratio
5：1：2 add in tea polyphenols and vitamin E mixing, obtain mixing antioxidant；
（4）According to the mass fraction, 8 parts of soybean lecithins, 1 part of cholesterol, 3 parts of Tween-80s, 5 parts of steps are taken（2）Spare deodorization
Liquid, 12 parts of mixing antioxidants, 60 parts of absolute ethyl alcohols, the PBS buffer solution of 20 parts of a concentration of 0.05mol/L, oscillation mixing 1h, rotation
Turn evaporation, in -3 DEG C, 350W ultrasound 5min, cross 0.2 μm of polycarbonate membrane, obtain suspension；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 12 parts of mixed liquor As, 8 parts of mixed liquid Bs, 20 parts of suspensions, 12
Part composite nutrient mixing, stirs 1h in 85 DEG C, pours into mold, in 25 DEG C of dryings for 24 hours, obtains fish oil soft capsule.
Embodiment 2
Salmon liver homogenate liquid：Salmon is taken out into liver, is washed 4 times in physiological saline, the salmon liver that will have been washed
It is dirty to be homogenized in -2 DEG C, take homogenate freezen protective.
Vitis davidii Foex schlempe：Purple autumn Vitis davidii Foex, Hunan Huachun Zhuangyuan Wines Co., Ltd..
Composite nutrient：According to the mass fraction, 13 parts of propolis are taken, 4 parts of Radix Angelicae Sinensis, 4 portions of aloes, 3 parts of matrimony vines mixing, grinding is even
Slurry, obtains composite nutrient.
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8.2, add in salmon liver
The phosphate of homogenate quality 2 ~ 5% is warming up to 43 DEG C, and 300 r/min are stirred, and 4h is digested, then at 93 DEG C of enzyme deactivations
10min, 8000 r/min centrifuge 10 min, take supernatant A in mass ratio 1：8 add in 60 DEG C of distilled water, and oscillation shakes up, and 8000
R/min centrifuges 12 min again, collects supernatant B；
（2）By supernatant B in mass ratio 10：4：0.2 adds in absolute ethyl alcohol and sodium hydroxide, is warming up to 50 DEG C of reaction 1.7h, rotation
Turn evaporation, take rotary evaporation object in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, and decolourize 2h, obtains de-
Color object takes decoloration object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 11h is kept in -5 DEG C, and filtering takes filtrate, puts
In deodorizing tower, 1h is kept, obtains deodorization liquid, it is spare；
（3）It takes spine grape wine ground-slag end in 1.5MPa, be passed through steam blasting 170s, collect powder in mass ratio 1 after steam blasting：
15 add in 80% ethanol solution, in 57 DEG C of ultrasound 40min, through 0.22 μm of organic filtering with microporous membrane, take filtrate, in mass ratio
5：1：2 add in tea polyphenols and vitamin E mixing, obtain mixing antioxidant；
（4）According to the mass fraction, 10 parts of soybean lecithins, 1 part of cholesterol, 3 parts of Tween-80s, 5 parts of steps are taken（2）Spare deodorization
Liquid, 13 parts of mixing antioxidants, 65 parts of absolute ethyl alcohols, the PBS buffer solution of 25 parts of a concentration of 0.05mol/L, oscillation mixing 1h, rotation
Turn evaporation, in -3 DEG C, 350W ultrasound 5min, cross 0.2 μm of polycarbonate membrane, obtain suspension；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 13 parts of mixed liquor As, 10 parts of mixed liquid Bs, 25 parts of suspensions, 16
Part composite nutrient mixing, stirs 1h in 87 DEG C, pours into mold, in 27 DEG C of dry 30h, obtain fish oil soft capsule.
Embodiment 3
Salmon liver homogenate liquid：Salmon is taken out into liver, is washed 5 times in physiological saline, the salmon liver that will have been washed
It is dirty to be homogenized in 4 DEG C, take homogenate freezen protective.
Vitis davidii Foex schlempe：Purple autumn Vitis davidii Foex, Hunan Huachun Zhuangyuan Wines Co., Ltd..
Composite nutrient：According to the mass fraction, 15 parts of propolis are taken, 5 parts of Radix Angelicae Sinensis, 6 portions of aloes, 4 parts of matrimony vines mixing, grinding is even
Slurry, obtains composite nutrient.
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8.5, add in salmon liver
The phosphate of homogenate quality 5% is warming up to 45 DEG C, and 300 r/min are stirred, and 5 h is digested, then at 95 DEG C of enzyme deactivations
15min, 8000 r/min centrifuge 10 min, take supernatant A in mass ratio 1：8 add in 70 DEG C of distilled water, and oscillation shakes up, and 8000
R/min centrifuges 15 min again, collects supernatant B；
（2）By supernatant B in mass ratio 10：4：0.3 adds in absolute ethyl alcohol and sodium hydroxide, is warming up to 55 DEG C of reaction 2h, rotation
Evaporation, takes rotary evaporation object in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, and decolourize 3h, must decolourize
Object takes decoloration object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 12 h are kept in -5 DEG C, and filtering takes filtrate, puts
In deodorizing tower, 2h is kept, obtains deodorization liquid, it is spare；
（3）It takes spine grape wine ground-slag end in 1.5MPa, be passed through steam blasting 180s, collect powder in mass ratio 1 after steam blasting：
15 add in 80% ethanol solution, in 60 DEG C of ultrasound 40min, through 0.22 μm of organic filtering with microporous membrane, take filtrate, in mass ratio
5：1：2 add in tea polyphenols and vitamin E mixing, obtain mixing antioxidant；
（4）According to the mass fraction, 12 parts of soybean lecithins, 2 parts of cholesterol, 4 parts of Tween-80s, 6 parts of steps are taken（2）Spare deodorization
Liquid, 15 parts of mixing antioxidants, 70 parts of absolute ethyl alcohols, the PBS buffer solution of 30 parts of a concentration of 0.05mol/L, oscillation mixing 2h, rotation
Turn evaporation, in -4 DEG C, 350W ultrasound 6min, cross 0.2 μm of polycarbonate membrane, obtain suspension；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 15 parts of mixed liquor As, 14 parts of mixed liquid Bs, 30 parts of suspensions, 18
Part composite nutrient mixing, stirs 2h in 90 DEG C, pours into mold, in 30 DEG C of dry 36h, obtain fish oil soft capsule.
Comparative example：The fish oil soft capsule of Shenzhen bio tech ltd production
Method：20 volunteers of selection carry out test-meal experiment, and instructions of taking is daily 1, after taking 45 days, according to SCT3504-
The variation of each detection project before 2006 detection experimenter's experiments and after experiment.（This is sentenced subject to average value）
The specific detection case of fish oil soft capsule such as table 1
Table 1
Note：Detection glutamic-pyruvic transaminase is about 16.00 ± 1.25 before experiment；Glutamic-oxalacetic transaminease is about 17.56 ± 0.8；Total protein is about
It is 56.10 ± 0.36；Blood glucose is about 4.51 ± 0.36.
After experiment, fish-oil capsule sensory test is carried out to experimenter, assessment item is from the state of fish-oil capsule, smell
It is evaluated with three aspects of mouthfeel.20% proportion of state, 30% proportion of smell, mouthfeel 50% proportion measure fish-oil capsule with this
Comprehensive score.
Without fishy smell（On 85）, it is acceptable（75 to 85 points）, it is unacceptable（Less than 75 points）.
Fish-oil capsule mouthfeel synthesis measuring such as table 2
Table 2
Note：The difference degree of data between different lowercase letters are handled after data of going together, difference is bigger, and letter is more rearward.
From the foregoing, it will be observed that the fish-oil capsule prepared by the present invention can effectively adjust function of human body situation, and preparation method
It is simple to operation, it is a kind of safety and the preparation method of efficient fish-oil capsule, is worthy to be popularized and uses.
Claims (6)
1. a kind of preparation method of fish oil soft capsule, which is characterized in that the preparation method includes the following steps：
（1）Take salmon liver homogenate liquid in mass ratio 1：2 add in distilled water, adjust pH to 8 ~ 8.5, and it is even to add in salmon liver
The phosphate of grind slurries quality 2 ~ 5%, is stirred, enzymolysis, in 90 ~ 95 DEG C of enzyme deactivation 5 ~ 15min, 8000 r/min centrifugations 10
Min takes supernatant A in mass ratio 1：8 add in 50 ~ 70 DEG C of distilled water, and oscillation shakes up, and 8000 r/min centrifuge 10 ~ 15 again
Min collects supernatant B；
（2）By supernatant B in mass ratio 10：4：0.2 ~ 0.3 adds in absolute ethyl alcohol and sodium hydroxide, reaction, and rotary evaporation takes rotation
Turn evaporant in mass ratio 1：5：3 add in carclazyte and activated carbon mixing, are placed in decoloration kettle, and decolourize 2 ~ 3h, obtains decoloration object, takes de-
Color object in mass ratio 1：1：7 add in urea and absolute ethyl alcohol mixing, and 10 ~ 12 h are kept in -5 DEG C, and filtering takes filtrate, is placed in de-
Smelly tower keeps 1 ~ 2h, obtains deodorization liquid, spare；
（3）Spine grape wine ground-slag end is taken to be passed through steam, powder in mass ratio 1 after steam blasting is collected in explosion：15 add in 80%
Ethanol solution, ultrasound, through 0.22 μm of organic filtering with microporous membrane, takes filtrate in mass ratio 5：1：2 add in tea polyphenols and vitamin E
Mixing, obtains mixing antioxidant；
（4）According to the mass fraction, 8 ~ 12 parts of soybean lecithins, 1 ~ 2 part of cholesterol, 3 ~ 4 parts of Tween-80s, 5 ~ 6 parts of steps are taken（2）
Spare deodorization liquid, 12 ~ 15 parts of mixing antioxidants, 60 ~ 70 parts of absolute ethyl alcohols, the PBS of 20 ~ 30 parts of a concentration of 0.05mol/L delay
Fliud flushing, oscillation 1 ~ 2h of mixing, rotary evaporation, ultrasound cross 0.2 μm of polycarbonate membrane, obtain suspension；
（5）Take carragheen in mass ratio 3：5 add in glycerine mixing, obtain mixed liquor A, take hydroxypropul starch in mass ratio 5：4 add
Enter purified water mixing, obtain mixed liquid B, according to the mass fraction, take 12 ~ 15 parts of mixed liquor As, 8 ~ 14 parts of mixed liquid Bs, 20 ~ 30 parts
Suspension, 12 ~ 18 parts of composite nutrient mixing, stirring pour into mold, in 25 ~ 30 DEG C of dry 24 ~ 36h, obtain fish oil soft capsule.
2. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（1）Middle salmon
Liver homogenate liquid is that salmon is taken out liver, is washed in physiological saline, and the salmon liver washed is even in -3 ~ 4 DEG C
Slurry, takes homogenate freezen protective；Enzymatic hydrolysis condition is 40 ~ 45 DEG C, and 300 r/min are stirred, and digests 4 ~ 5 h.
3. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（2）Middle reaction item
Part is 45 ~ 55 DEG C of 1.5 ~ 2h of reaction.
4. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（3）Middle steam is quick-fried
Broken condition：1.5MPa, 150 ~ 180s of steam blasting.
5. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（4）Middle ultrasound item
Part is：In -3 ~ -4 DEG C, 5 ~ 6min of 350W ultrasounds.
6. the preparation method of fish oil soft capsule according to claim 1, which is characterized in that the step（5）In compound battalion
Foster agent is：According to the mass fraction, 12 ~ 15 parts of propolis are taken, 4 ~ 5 parts of Radix Angelicae Sinensis, 3 ~ 6 portions of aloes, 2 ~ 4 parts of matrimony vines mixing, grinding homogenate,
Obtain composite nutrient.
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| CN108913674A (en) * | 2018-08-10 | 2018-11-30 | 中国海洋大学 | A kind of lipid hydrolyzing enzyme and its application in EPA/DHA glycerol Lipase absobed |
| CN110982854A (en) * | 2020-02-26 | 2020-04-10 | 山东东方海洋科技股份有限公司 | Preparation method of salmon viscera fish oil |
| CN113543653A (en) * | 2018-12-14 | 2021-10-22 | 帝斯曼知识产权资产管理有限公司 | Polyunsaturated fatty acid-containing food ingredient having enhanced palatability and method of making same |
| CN113575698A (en) * | 2021-08-03 | 2021-11-02 | 上海健康医学院 | Fish oil soft capsule and preparation method and application thereof |
| CN116869166A (en) * | 2023-07-24 | 2023-10-13 | 广东海洋大学 | Fish oil emulsion and preparation method and application thereof |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0225447A (en) * | 1988-07-13 | 1990-01-26 | Nippon Oil & Fats Co Ltd | Production of highly unsaturated fatty acids |
| CN1965813A (en) * | 2006-08-24 | 2007-05-23 | 王超敏 | Compound soft capsule of propolis |
| CN101049297A (en) * | 2006-04-06 | 2007-10-10 | 北京百慧生化制药有限责任公司 | High DNA type fatty ethyl ester in high purity, manufacturing method and preparation |
| CN101516202A (en) * | 2006-09-14 | 2009-08-26 | 日新Wells株式会社 | Glyceride oil composition from fish oil and preparation method thereof |
| CN101579118A (en) * | 2009-03-26 | 2009-11-18 | 大山合集团有限公司 | Health food of black fungus soft capsule |
| CN102181498A (en) * | 2011-03-25 | 2011-09-14 | 江阴贝科生物科技有限公司 | Method for preparing phosphatidylcholine type omega-3 unsaturated fatty acid by using enzyme method |
| CN102488718A (en) * | 2011-12-27 | 2012-06-13 | 深圳市麦金利实业有限公司 | Fish oil soft capsule and its preparation method |
| CN103238840A (en) * | 2013-05-16 | 2013-08-14 | 汤臣倍健股份有限公司 | Ginkgo biloba extract and fish oil soft capsule and preparation method thereof |
| CN103349289A (en) * | 2013-06-14 | 2013-10-16 | 芜湖市诺康生物科技有限公司 | Preparation method of fish oil soft capsules |
| CN103656098A (en) * | 2013-11-21 | 2014-03-26 | 荣成百合生物技术有限公司 | Composite fish oil soft capsule and preparation method thereof |
| CN104147116A (en) * | 2014-08-30 | 2014-11-19 | 中山百鸥医药科技有限公司 | Omega-3 fish oil soft capsule |
| CN104189014A (en) * | 2014-09-03 | 2014-12-10 | 汤臣倍健股份有限公司 | Soybean phospholipid soft capsule and preparation method thereof |
| CN104666370A (en) * | 2013-11-30 | 2015-06-03 | 烟台巨先药业有限公司 | Deep sea fish oil soft capsules and preparation method thereof |
| CN105434393A (en) * | 2014-09-25 | 2016-03-30 | 四川国为制药有限公司 | Soft capsule containing high-content omega-3 polyunsaturated fatty acid |
| WO2017197453A1 (en) * | 2016-05-17 | 2017-11-23 | Deakin University | Microencapsulated omega-3 polyunsaturated fatty acid glyceride compositions and processes for preparing the same |
-
2017
- 2017-12-18 CN CN201711363537.4A patent/CN108208685A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0225447A (en) * | 1988-07-13 | 1990-01-26 | Nippon Oil & Fats Co Ltd | Production of highly unsaturated fatty acids |
| CN101049297A (en) * | 2006-04-06 | 2007-10-10 | 北京百慧生化制药有限责任公司 | High DNA type fatty ethyl ester in high purity, manufacturing method and preparation |
| CN1965813A (en) * | 2006-08-24 | 2007-05-23 | 王超敏 | Compound soft capsule of propolis |
| CN101516202A (en) * | 2006-09-14 | 2009-08-26 | 日新Wells株式会社 | Glyceride oil composition from fish oil and preparation method thereof |
| CN101579118A (en) * | 2009-03-26 | 2009-11-18 | 大山合集团有限公司 | Health food of black fungus soft capsule |
| CN102181498A (en) * | 2011-03-25 | 2011-09-14 | 江阴贝科生物科技有限公司 | Method for preparing phosphatidylcholine type omega-3 unsaturated fatty acid by using enzyme method |
| CN102488718A (en) * | 2011-12-27 | 2012-06-13 | 深圳市麦金利实业有限公司 | Fish oil soft capsule and its preparation method |
| CN103238840A (en) * | 2013-05-16 | 2013-08-14 | 汤臣倍健股份有限公司 | Ginkgo biloba extract and fish oil soft capsule and preparation method thereof |
| CN103349289A (en) * | 2013-06-14 | 2013-10-16 | 芜湖市诺康生物科技有限公司 | Preparation method of fish oil soft capsules |
| CN103656098A (en) * | 2013-11-21 | 2014-03-26 | 荣成百合生物技术有限公司 | Composite fish oil soft capsule and preparation method thereof |
| CN104666370A (en) * | 2013-11-30 | 2015-06-03 | 烟台巨先药业有限公司 | Deep sea fish oil soft capsules and preparation method thereof |
| CN104147116A (en) * | 2014-08-30 | 2014-11-19 | 中山百鸥医药科技有限公司 | Omega-3 fish oil soft capsule |
| CN104189014A (en) * | 2014-09-03 | 2014-12-10 | 汤臣倍健股份有限公司 | Soybean phospholipid soft capsule and preparation method thereof |
| CN105434393A (en) * | 2014-09-25 | 2016-03-30 | 四川国为制药有限公司 | Soft capsule containing high-content omega-3 polyunsaturated fatty acid |
| WO2017197453A1 (en) * | 2016-05-17 | 2017-11-23 | Deakin University | Microencapsulated omega-3 polyunsaturated fatty acid glyceride compositions and processes for preparing the same |
Non-Patent Citations (3)
| Title |
|---|
| 何娜: "鲢鱼鱼油多不饱和脂肪酸纳米脂质体的制备及其性质研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑（月刊）》 * |
| 刘书成等: "脂肪酶水解低值鱼油富集EPA和DHA甘油酯的研究", 《中国粮油学报》 * |
| 杨小侠等: "新型植物卡拉胶软胶囊处方及工艺研究", 《亚太传统医药》 * |
Cited By (8)
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|---|---|---|---|---|
| CN108913674A (en) * | 2018-08-10 | 2018-11-30 | 中国海洋大学 | A kind of lipid hydrolyzing enzyme and its application in EPA/DHA glycerol Lipase absobed |
| CN113543653A (en) * | 2018-12-14 | 2021-10-22 | 帝斯曼知识产权资产管理有限公司 | Polyunsaturated fatty acid-containing food ingredient having enhanced palatability and method of making same |
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| CN110982854B (en) * | 2020-02-26 | 2020-06-23 | 山东东方海洋科技股份有限公司 | Preparation method of salmon viscera fish oil |
| CN113575698A (en) * | 2021-08-03 | 2021-11-02 | 上海健康医学院 | Fish oil soft capsule and preparation method and application thereof |
| CN113575698B (en) * | 2021-08-03 | 2023-12-22 | 上海健康医学院 | Fish oil soft capsule and preparation method and application thereof |
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