CN108207942A - A kind of composite microbial microbial inoculum for preventing agrobacterium - Google Patents
A kind of composite microbial microbial inoculum for preventing agrobacterium Download PDFInfo
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- CN108207942A CN108207942A CN201810020798.4A CN201810020798A CN108207942A CN 108207942 A CN108207942 A CN 108207942A CN 201810020798 A CN201810020798 A CN 201810020798A CN 108207942 A CN108207942 A CN 108207942A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses a kind of composite microbial microbial inoculums for preventing agrobacterium, micro- bacteria-promoting agent is for carrier with bentonite, magnesium sulfate, calcium carbonate, middle trace element, it is implanted in the mixture of bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, lactic acid bacteria, bacillus subtilis, Paecilomyces lilacinus and streptomyces microflavus bacterium powder, it pours into mixing machine and is stirred made from 20 minutes at room temperature, the technical principle for preventing agrobacterium is heteroauxin, gibberellin, the basic element of cell division, antibiotic that thalline secrets out of；The present invention overcome single micro- bacteria-promoting agent, work slow, the unconspicuous shortcoming of effect；Plant is promoted to improve 5 15% to nitrogen, phosphorus, potassium absorption rate；While plant growth, volume increase 10 15% is promoted, it can induce plant and generate resistance, enhance the disease-resistant of plant, cold-resistant, drought-resistant ability, it is with obvious effects to prevent vegetables, fruit tree soil-borne disease, continuous cropping, control rate especially presses down the rate of killing to the agrobacterium for causing fruit tree root knot and reaches 95% up to 70 90%.
Description
Technical field
The present invention relates to a kind of micro- bacteria-promoting agent, specially a kind of composite microbial microbial inoculum for preventing agrobacterium belongs to agricultural
Microbial inoculum applied technical field.
Background technology
With the fast development of agricultural, due to needing commonly using chemical fertilizer, pesticide so that soil ecosystem has received sternly
The destruction of weight, in the vicious circle into soil regime so that soil hardening, disease pest increase.Agrobacterium in soil is leather
Lan Shi negative bacteriums, aerobic, asporogenic bacillus belong to Rhizobiaceae, but not fixed nitrogen, and some peritrichas have
Single trichobacteria is distributed in soil, and the overwhelming majority can make plant carcinogenic, seriously affect the growth of plant, it is impossible to make crops sufficient
Nutrient needed for absorbing, is restricted the growth of crops, and some crops is made to morph, to the body of people
Injury can be brought.
The mode that traditional agrobacterium is administered is single, and the microbial bacterial agent of use works slowly.Effect is poor, it is impossible to make plant
Good absorption is carried out, and the agrobacterium in soil makes plant disease-resistant, drought resisting, cold tolerance for elements such as nitrogen, phosphorus, potassium
It is weak.
Therefore, a kind of composite microbial microbial inoculum for preventing agrobacterium is proposed regarding to the issue above.
Invention content
The purpose of the present invention is that solve the above-mentioned problems and provides a kind of composite microbial bacterium for preventing agrobacterium
Agent, micro- bacteria-promoting agent secretion heteroauxin, gibberellin, the basic element of cell division, antibiotic substance promote plant capillary root development, new old
Metabolism promotes cell division, improves absorbability of the plant to nutrient, and enhancing plant resistance to environment stress, detoxication change soil ring
Border, P in soil H.
The present invention is achieved through the following technical solutions above-mentioned purpose, a kind of composite microbial microbial inoculum for preventing agrobacterium,
Micro- bacteria-promoting agent be with bentonite, magnesium sulfate, calcium carbonate, it is middle trace element for carrier, be implanted to bacillus megaterium,
Clothing bacillus, Paenibacillus polymyxa, lactic acid bacteria, bacillus subtilis, Paecilomyces lilacinus and streptomyces microflavus bacterium powder it is mixed
It closes in object, it is thalline secretion to pour into the technical principle for being stirred in mixing machine and made from 20 minutes, preventing agrobacterium at room temperature
Heteroauxin, gibberellin, the basic element of cell division, the antibiotic gone out；
Wherein, the bentonite, magnesium sulfate, calcium carbonate mass ratio are 2: 1: 2, by the mixture after proportioning with closed
Stainless steel blender, which mixes 30 minutes, becomes carrier；The carrier is broken into more than 100 mesh with raymond grinding powder, is packed into iron pan and is put into
After forty minutes, taking-up iron pan is cooled to room temperature spare for baking in the drying box that temperature is 160 DEG C；
Wherein, bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, lactic acid bacteria, bacillus subtilis, light
Purple Paecilomyces varioti, streptomyces microflavus bacterium powder mass ratio are 3: 1: 1: 3: 1: 1: 5.
Preferably, the preparation process of the bacillus megaterium is as follows：Corn flour is added in 1000ml water：20g, it is yellow
Bean powder：10g, potassium hydrogen phosphate：1.5g, epsom salt：1.5g, calcium carbonate： 1.5g；It is 7.5 in PH, temperature is issued for 32 DEG C
Ferment 72 hours recycles vacuum drying agent to be produced into freeze-dried powder.
Preferably, the preparation process of the bacillus licheniformis is as follows：Sucrose is added in 1000ml water：10g, ferment
Female cream：5g, sodium chloride：10g, in PH7.0, temperature is to ferment 24 hours at 37 DEG C, and vacuum drying agent is recycled to be produced into freeze-drying
Powder.
Preferably, the preparation process of the Paenibacillus polymyxa is as follows：Peptone is added in 1000ml water：25g, chlorine
Change sodium：1.25g disodium hydrogen phosphate：It ferments 48 hours at 0.5g is 30 DEG C in PH7.5, inoculum concentration 10%, temperature, then profit
Freeze-dried powder is produced into vacuum drying agent.
Preferably, the preparation process of the lactic acid bacteria is as follows：Peptone is added in 1000ml water：10g, yeast extract：
10g, beef extract：10g, potassium dihydrogen phosphate：2g, glucose：10g, maltose：10g, ammonium citrate：2g, magnesium sulfate：5g, sulfuric acid
Manganese：2g, tomato juice：40g ferments 48 hours at being 30 DEG C in PH6.5, inoculum concentration 10%, temperature, recycles vacuum drying agent
It is produced into freeze-dried powder.
Preferably, the preparation process of the Paecilomyces lilacinus is as follows：
1) by old rice: glucose: cornstarch: water=24: according to mass ratio be uniformly mixed at 16: 20: 40；
2) it is 28 DEG C uniformly mixed material to be placed on temperature, and humid control ferments 96 hours under 30-40%, turns over
It is primary；
3) until 1 day or so grows conidia powder, the conidia powder of collection is poured into sterile wide-mouth bottle, is sealed with cotton swab
It is good, it is fitly put into vacuum drying kettle, Fu Nei lower floors spread the silica gel of discoloration, and the drying kettle of sealing keeps temperature 30 DEG C -35
DEG C, negative pressure is dried in vacuo within 0.088-0.09MPa, until the silica gel of discoloration restores, dry bacterium powder spare can be mixed
Match.
Preferably, the preparation process of the streptomyces microflavus is as follows：Bean cake powder：4%, sawdust：12%, sweet potato flour：
12%, bentonite：35%, lime：2%, inoculum concentration 5%, PH7 room temperature under ferment 7 days, be produced into vacuum drying agent
Freeze-dried powder is spare.
Preferably, the preparation process of the bacillus subtilis is as follows：Glucose：1.5%, dregs of beans：3%, starch：
0.1%, calcium carbonate：0.01%, manganese sulfate：0.01%, yeast extract：0.02%, iron chloride：0.01%, in the case where temperature is 37 DEG C
Fermentation 72 hours, freeze-dried powder is produced into using agent is dried in vacuo.
Preferably, living bacteria count is not less than 200,000,000/g in micro- bacteria-promoting agent.
Preferably, the trace element is calcium, iron, sulphur, magnesium.
The present invention achieves significantly technological progress.
Bentonite, magnesium sulfate, calcium carbonate are carried out science combination by the present invention, and microbial activity can be kept to be up to 24
A month, plant is promoted to improve 5-15% to nitrogen, phosphorus, potassium absorption rate；By bacillus megaterium, bacillus licheniformis, glue more
Series bacillus, lactic acid bacteria, bacillus subtilis, Paecilomyces lilacinus, with streptomyces microflavus bacterium powder according to 3: 1: 1: 3: 1: 1: 5 with
Carrier stirs evenly poured into mixing machine with reference to 20 minutes successively, while plant growth, volume increase 10-15% is promoted, can induce
Plant generates resistance, enhances the disease-resistant of plant, cold-resistant, drought-resistant ability, and prevention vegetables, fruit tree soil-borne disease, continuous cropping effect are bright
Aobvious, control rate reaches 70-90%, and especially pressing down the rate of killing to the agrobacterium for causing fruit tree root knot reaches 95%.
Due to containing 7 kinds of micro- bacteria-promoting agents in the present invention, heteroauxin, gibberellin, the basic element of cell division, antibiotic can be secreted
Substance promotes plant capillary root development, metabolism, promotes cell division, improves absorbability of the plant to nutrient, and enhancing is planted
Object resistance, detoxication change soil environment, P in soil H；
It is the main microbial inoculum of soil nutrient toxin degradation containing the micro- bacteria-promoting agent bacillus of detoxifying in the present invention, gemma bar
Bacterium can promote soil texture to be formed, and improve soil physical properties, improve fertilizer conservation, the water holding capacity of soil；Contain in the present invention
The necessary middle and trace element of plant growth, can be effectively improved in the nutrient growth and reproductive growth of plant, cause due to nutritional deficiency
Various diseases, fungus damage；
The present invention has embodied a concentrated reflection of the plurality of advantages of solid composite microbial microbial inoculum, such as：Improve crop system resistance, enhancing is exempted from
Epidemic disease power, section medicine, efficient, environmental protection, high yield, high-quality, drought resisting, cold-resistant, soil-borne disease agrobacterium is propagated in kill, raising seedling survives
Rate plays huge effect in agriculture cost efficiency and water-saving and environmental protection field.
Specific embodiment
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
A kind of composite microbial microbial inoculum for preventing agrobacterium, micro- bacteria-promoting agent is with bentonite, magnesium sulfate, carbonic acid
Calcium, middle trace element are implanted to bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, lactic acid bacteria, withered for carrier
In the mixture of careless bacillus, Paecilomyces lilacinus and streptomyces microflavus bacterium powder, pour into 20 points are stirred in mixing machine at room temperature
Made from clock, the technical principle for preventing agrobacterium is heteroauxin, gibberellin, the basic element of cell division, antibiosis that thalline secrets out of
Element；
Wherein, the bentonite, magnesium sulfate, calcium carbonate mass ratio are 2: 1: 2, by the mixture after proportioning with closed
Stainless steel blender, which mixes 30 minutes, becomes carrier；The carrier is broken into more than 100 mesh with raymond grinding powder, is packed into iron pan and is put into
After forty minutes, taking-up iron pan is cooled to room temperature spare for baking in the drying box that temperature is 160 DEG C；
Wherein, bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, lactic acid bacteria, bacillus subtilis, light
Purple Paecilomyces varioti, streptomyces microflavus bacterium powder mass ratio are 3: 1: 1: 3: 1: 1: 5.
The preparation process of the bacillus megaterium is as follows：Corn flour is added in 1000ml water：20g, analysis for soybean powder：
10g, potassium hydrogen phosphate：1.5g, epsom salt：1.5g, calcium carbonate：1.5g；It is 7.5 in PH, temperature is that fermentation 72 is small at 32 DEG C
When, vacuum drying agent is recycled to be produced into freeze-dried powder.
The preparation process of the bacillus licheniformis is as follows：Sucrose is added in 1000ml water：10g, yeast extract：5g,
Sodium chloride：10g, in PH7.0, temperature is to ferment 24 hours at 37 DEG C, and vacuum drying agent is recycled to be produced into freeze-dried powder.
The preparation process of the Paenibacillus polymyxa is as follows：Peptone is added in 1000ml water：25g, sodium chloride：
1.25g disodium hydrogen phosphate：It ferments 48 hours at 0.5g is 30 DEG C in PH7.5, inoculum concentration 10%, temperature, recycles vacuum
Drier is produced into freeze-dried powder.
The preparation process of the lactic acid bacteria is as follows：Peptone is added in 1000ml water：10g, yeast extract：10g, beef
Cream：10g, potassium dihydrogen phosphate：2g, glucose：10g, maltose：10g, ammonium citrate：2g, magnesium sulfate：5g, manganese sulfate：2g, kind
Tomato juice：40g ferments 48 hours at being 30 DEG C in PH6.5, inoculum concentration 10%, temperature, vacuum drying agent is recycled to be produced into freeze-drying
Powder.
The preparation process of the Paecilomyces lilacinus is as follows：
1) by old rice: glucose: cornstarch: water=24: according to mass ratio be uniformly mixed at 16: 20: 40；
2) it is 28 DEG C uniformly mixed material to be placed on temperature, and humid control ferments 96 hours under 30-40%, turns over
It is primary；
3) until 1 day or so grows conidia powder, the conidia powder of collection is poured into sterile wide-mouth bottle, is sealed with cotton swab
It is good, it is fitly put into vacuum drying kettle, Fu Nei lower floors spread the silica gel of discoloration, and the drying kettle of sealing keeps temperature 30 DEG C -35
DEG C, negative pressure is dried in vacuo within 0.088-0.09MPa, until the silica gel of discoloration restores, dry bacterium powder spare can be mixed
Match.
The preparation process of the streptomyces microflavus is as follows：Bean cake powder：4%, sawdust：12%, sweet potato flour：12%, swelling
Soil：35%, lime：2%, inoculum concentration 5%, PH7 room temperature under ferment 7 days, it is spare to be produced into freeze-dried powder with vacuum drying agent.
The preparation process of the bacillus subtilis is as follows：Glucose：1.5%, dregs of beans：3%, starch：0.1%, carbon
Sour calcium：0.01%, manganese sulfate：0.01%, yeast extract：0.02%, iron chloride：0.01%, fermentation 72 is small at being 37 DEG C in temperature
When, it is produced into freeze-dried powder using agent is dried in vacuo.
Living bacteria count is not less than 200,000,000/g in micro- bacteria-promoting agent.
The trace element in the present invention is calcium, iron, sulphur, magnesium.
Embodiment one：
The composite microbial microbial inoculum 4000g of the present invention is taken, is divided into 400 groups, every group of 10g composite microbials microbial inoculum is dropped in respectively
In the root soil for planting 100 apple trees in same area, 100 pear trees, 100 peach, 100 jujube trees, it will pass
The composite microbial microbial inoculum 4000g of system, is divided into 400 groups, and every group of 10g composite microbials microbial inoculum is dropped in plantation respectively in same zone
100 apple trees, 100 pear trees, 100 peach in domain, 100 jujube trees root soil in；By statistics, input is originally
While the composite microbial microbial inoculum of invention improves 15% than fruit tree growth, the yield-increasing efficiency of traditional microbial bacteria, control rate reaches
70%, the rate of killing especially is pressed down to the agrobacterium for causing fruit tree root knot and reaches 95%.
Embodiment two：
The composite microbial microbial inoculum 8000g of the present invention is taken, is divided into 8 groups, every group of 1000g composite microbials microbial inoculum is dropped in respectively
It, will be traditional compound micro- in the root soil for planting 2 mu of eggplants in same area, 2 mu of gingers, 2 mu of cucumber, 2 mu of cowpeas
Bacteria-promoting agent 8000g is divided into 8 groups, and every group of 1000g composite microbials microbial inoculum is dropped in 2 mu eggplants of the plantation in same area respectively
Son, 2 mu of baby cabbages, 2 mu of cucumber, 2 mu of cowpeas root soil in；By statistics, the composite microbial microbial inoculum ratio of the present invention is put into
While the vegetable growth of traditional microbial bacteria, yield-increasing efficiency improve 15%, control rate is up to 85%.
Embodiment three：
The composite microbial microbial inoculum 8000g of the present invention is taken, is divided into 4 groups, every group of 2000g composite microbials microbial inoculum is dropped in respectively
In the root soil for planting 2 mu of wheats in same area, 2 mu of corns, by traditional composite microbial microbial inoculum 4000g, it is divided into 4
Every group of 1000g composite microbials microbial inoculum is dropped in the root soil of 2 mu rice of the plantation in same area, 2 mu of corns by group respectively
In root soil in earth；By statistics, put into the present invention composite microbial microbial inoculum than traditional microbial bacteria vegetable growth,
While yield-increasing efficiency improves 12%, control rate is up to 86%.
Example IV：
The composite microbial microbial inoculum 6000g of the present invention is taken, is divided into 4 groups, every group of 1500g composite microbials microbial inoculum is dropped in respectively
In the root soil for planting 2 mu of campanulaceaes in same area, 2 mu of Rhizoma Gastrodiaes, by traditional composite microbial microbial inoculum 6000g, it is divided into 4
Every group of 1500g composite microbials microbial inoculum is dropped in the root soil of 2 mu campanulaceaes of the plantation in same area, 2 mu of Rhizoma Gastrodiaes by group respectively
In earth；By statistics, vegetable growth of the composite microbial microbial inoculum than traditional microbial bacteria of the present invention is put into, yield-increasing efficiency improves
While 13%, control rate is up to 82%.
Micro- bacteria-promoting agent of the present invention promotes cell division, improves absorbability of the plant to nutrient, enhancing plant resistance to environment stress, solution
Toxic action changes soil environment, P in soil H；It is the main of soil nutrient toxin degradation containing micro- bacteria-promoting agent bacillus of detoxifying
Microbial inoculum, bacillus can promote soil texture to be formed, and improve soil physical properties, improve fertilizer conservation, the water holding capacity of soil；This
It containing the necessary middle and trace element of plant growth in invention, can be effectively improved in the nutrient growth and reproductive growth of plant, because lacking
Plain and initiation various diseases, fungus damage.
It is obvious to a person skilled in the art that the present invention is not limited to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (10)
1. a kind of composite microbial microbial inoculum for preventing agrobacterium, it is characterised in that：Micro- bacteria-promoting agent is with bentonite, sulfuric acid
Magnesium, calcium carbonate, middle trace element are carrier, are implanted to bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, breast
Sour bacterium, bacillus subtilis, Paecilomyces lilacinus and streptomyces microflavus bacterium powder mixture in, pour into mixing machine stir at room temperature
Mix made from 20 minutes, prevent agrobacterium technical principle be thalline secret out of heteroauxin, gibberellin, cell division
Element, antibiotic；
Wherein, the bentonite, magnesium sulfate, calcium carbonate mass ratio are 2: 1: 2, by the mixture after proportioning with closed stainless
Steel blender, which mixes 30 minutes, becomes carrier；The carrier is broken into more than 100 mesh with raymond grinding powder, and loading iron pan is put into temperature and is
After forty minutes, taking-up iron pan is cooled to room temperature spare for baking in 160 DEG C of drying box；
Wherein, bacillus megaterium, bacillus licheniformis, Paenibacillus polymyxa, lactic acid bacteria, bacillus subtilis, pale purple plan
Mould, streptomyces microflavus bacterium powder mass ratio are 3: 1: 1: 3: 1: 1: 5.
2. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：Described is huge
The preparation process of bacillus is as follows：Corn flour is added in 1000ml water：20g, analysis for soybean powder：10g, potassium hydrogen phosphate：1.5g, seven
Water magnesium sulfate：1.5g, calcium carbonate：1.5g；It is 7.5 in PH, temperature is to ferment 72 hours at 32 DEG C, recycles vacuum drying agent life
Produce into freeze-dried powder.
3. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：The lichens
The preparation process of bacillus is as follows：Sucrose is added in 1000ml water：10g, yeast extract：5g, sodium chloride：10g, in PH7.0,
Temperature is to ferment 24 hours at 37 DEG C, and vacuum drying agent is recycled to be produced into freeze-dried powder.
4. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：More viscous classes
The preparation process of bacillus is as follows：Peptone is added in 1000ml water：25g, sodium chloride：1.25g disodium hydrogen phosphate：0.5g,
It ferments 48 hours at being 30 DEG C in PH7.5, inoculum concentration 10%, temperature, vacuum drying agent is recycled to be produced into freeze-dried powder.
5. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：The lactic acid
The preparation process of bacterium is as follows：Peptone is added in 1000ml water：10g, yeast extract：10g, beef extract：10g, potassium dihydrogen phosphate：
2g, glucose：10g, maltose：10g, ammonium citrate：2g, magnesium sulfate：5g, manganese sulfate：2g, tomato juice：40g, PH6.5,
Inoculum concentration 10%, temperature are to ferment 48 hours at 30 DEG C, and vacuum drying agent is recycled to be produced into freeze-dried powder.
6. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：Described is pale purple
The preparation process of Paecilomyces varioti is as follows：
1) by old rice: glucose: cornstarch: water=24: according to mass ratio be uniformly mixed at 16: 20: 40；
2) it is 28 DEG C uniformly mixed material to be placed on temperature, and humid control ferments 96 hours under 30-40%, overturns one
It is secondary；
3) until 1 day or so grows conidia powder, the conidia powder of collection is poured into sterile wide-mouth bottle, is sealed with cotton swab, it is whole
It is put into together in vacuum drying kettle, Fu Nei lower floors spread the silica gel of discoloration, and the drying kettle of sealing keeps 30 DEG C -35 DEG C of temperature, bears
It is pressed within 0.088-0.09MPa and is dried in vacuo, until the silica gel of discoloration restores, dry bacterium powder can spare mixture.
7. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：The thin Huang
The preparation process of streptomycete is as follows：Bean cake powder：4%, sawdust：12%, sweet potato flour：12%, bentonite：35%, lime：2%,
Inoculum concentration 5%, PH7 room temperature under ferment 7 days, it is spare to be produced into freeze-dried powder with vacuum drying agent.
8. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：The withered grass
The preparation process of bacillus is as follows：Glucose：1.5%, dregs of beans：3%, starch：0.1%, calcium carbonate：0.01%, manganese sulfate：
0.01%, yeast extract：0.02%, iron chloride：0.01%, it ferments 72 hours at being 37 DEG C in temperature, is given birth to using agent is dried in vacuo
Produce into freeze-dried powder.
9. a kind of composite microbial microbial inoculum for preventing agrobacterium according to claim 1, it is characterised in that：Micro- life
Living bacteria count is not less than 200,000,000/g in microbial inoculum.
10. according to a kind of composite microbial microbial inoculum for preventing agrobacterium of claim 1-9 any one of them, it is characterised in that：
The trace element is calcium, iron, sulphur, magnesium.
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| CN201810020798.4A CN108207942A (en) | 2018-01-10 | 2018-01-10 | A kind of composite microbial microbial inoculum for preventing agrobacterium |
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| CN201810020798.4A CN108207942A (en) | 2018-01-10 | 2018-01-10 | A kind of composite microbial microbial inoculum for preventing agrobacterium |
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| CN201810020798.4A Pending CN108207942A (en) | 2018-01-10 | 2018-01-10 | A kind of composite microbial microbial inoculum for preventing agrobacterium |
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| CN115074291A (en) * | 2022-07-07 | 2022-09-20 | 天津科技大学 | Bacillus megaterium capable of improving active ingredients and oxidation resistance of fermented platycodon grandiflorum, method and application |
| CN115074291B (en) * | 2022-07-07 | 2023-08-22 | 天津科技大学 | Bacillus megaterium capable of improving active ingredient and antioxidant capacity of fermented platycodon grandiflorum, method and application |
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