CN108192790A - Dendrobium officinale alcohol and preparation method thereof - Google Patents
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- CN108192790A CN108192790A CN201810183309.7A CN201810183309A CN108192790A CN 108192790 A CN108192790 A CN 108192790A CN 201810183309 A CN201810183309 A CN 201810183309A CN 108192790 A CN108192790 A CN 108192790A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
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Abstract
The invention discloses a kind of preparation method of Dendrobium officinale alcohol, including after dendrobium candidum original powder is mixed with base liquor, being digested, after acidolysis, neutralization and filtering by concentration, amylase, then allocate with base liquor to obtain the Dendrobium officinale alcohol.The invention also discloses a kind of Dendrobium officinale alcohol, wherein, 12 18g of dendrobium candidum original powder is contained in every liter of Dendrobium officinale alcohol.The dendrobium candidum original powder of the present invention is mature dendrobium candidum stem item next year in 2 to April of picking, by drying or frozen dried, the mesh number for being crushed to cell again is less than 500 mesh, then the alcohol-soluble of Dendrobium officinale polysaccharide is solved the problems, such as along with the acidolysis collective effect of hydrochloric acid by the enzymolysis of alpha amylase, dendrobium candidum effective active composition is given full play to, people is allowed to be absorbed when drinking.It is not only simple for process relative to the prior art but also safe and reliable, it is more advantageous to realizing industrialization development.
Description
Technical field
The invention belongs to food technology fields, and in particular to a kind of Dendrobium officinale alcohol and preparation method thereof.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is orchid family Dendrobium perennial herb
Plant also known as ribbed hedyotis herb, iron sheet orchid etc., civil also known as " a thousand pieces of gold grass " " soft gold ", are listed in first of " Chinese nine big celestial grass ", are
One of traditional rare medicinal herbs.The main component of dendrobium candidum is polysaccharide, amino acid, alkaloids, anthocyanidin etc., and wherein the stem of noble dendrobium is more
The content of sugar is up to more than 25%, has the effects that improve immune function of human body, enriching yin, blood pressure lowering, antifatigue, anti-aging.
《Compendium of Materia Medica》It records：Stem of noble dendrobium tonifying five zang organs, thick stomach；Also the stem of noble dendrobium is recorded in modern drug encyclopedia has reinforcing stomach reg fluid, nourishing Yin and clearing heat
Effect is hindered, dry polydipsia, deficiency of stomach-Yin for pyreticosis Tianjin, and deficiency of food is retched, and abnormal heat is not moved back after being ill, fire excess from yin deficiency, hectic fever due to yin labor heat,
Mesh is secretly unknown, muscles and bones impotence, has good medical value.
In recent years, with the promotion of people's health care consciousness, various health liquors constantly emerge in large numbers, using dendrobium candidum as raw material system
Into healthy medicated wine also gradually grow up on the market.Modern dendrobium candidum processing method is mostly to dry or be lyophilized or process
Powdering, exists during actually utilizing and meets the characteristics of water viscosity is strong, and entrance eats inconvenience, and dry matter dendrobium candidum makes
Used time is mostly that decocting or steeping in wine, principle active component polysaccharide etc. exist and be insoluble in the characteristics of water is insoluble in ethyl alcohol again, generally
Polysaccharose substance has larger affinity due to containing a large amount of polar group in its molecule, for hydrone；Exactly
Due to particularity of the polysaccharide on solubility property, resulting in the aqueous solution of polysaccharide compound has bigger viscosity even shape
Into gel, so dendrobium extract is more sticky.Therefore, the brewed Dendrobium officinale alcohol of traditional handicraft there are wine liquid muddiness and is divided always
The phenomenon that layer.In the invention application No. is 201210012761.X, a kind of dendrobium candidum health liquor is disclosed, wherein comprising more
Kind medicinal plant, obtains by impregnating, extracting, stirring and filtering, and is relatively suitble to family brew, for industrialized production
It is not appropriate for.Furthermore active ingredient cannot fully play, it is impossible to reach the health-care effect of Dendrobium officinale alcohol.Application No. is
In the invention of CN201710688649.0, a kind of dendrobium candidum wine preparing process is disclosed, although solving active ingredient activity
The problem of being difficult to play, still, technics comparing, are complicated, and the time that entire process for preparation needs is also very long, and required condition is more
Complexity, it is for industrialized production or not satisfactory.
Invention content
Present invention aim to address it is at least the above the defects of, and the advantages of illustrating after providing.
In order to realize object of the present invention and other advantages, a kind of Dendrobium officinale alcohol is now provided, can not only will be had
The activity of effect ingredient gives full play to fusion in wine, and its brewing method is simple, it is short to take, and is extremely suitable for industrial production.
In other words, the invention discloses a kind of preparation methods of Dendrobium officinale alcohol, it is characterised in that：By dendrobium candidum original powder
It after being mixed with base liquor, is digested, after acidolysis, neutralization and filtering by concentration, amylase, then with base liquor allocates to obtain described
Dendrobium officinale alcohol.
Preferably, in the preparation method of the Dendrobium officinale alcohol, using hydrochloric acid carry out acidolysis, using sodium hydroxide into
Row neutralizes.
Preferably, in the preparation method of the Dendrobium officinale alcohol, the base liquor is alcoholic strength 45-50 pure rice wines or wine
The pure liquor made from sorghums of precision 50-55.
Preferably, in the preparation method of the Dendrobium officinale alcohol, by mature iron next year in 2 months to April
Skin stem of Dendrobium item, after picking through drying or be lyophilized then, be crushed to mesh number be 400-500 powder obtain described in iron sheet
Stem of noble dendrobium original powder.
Preferably, in the preparation method of the Dendrobium officinale alcohol, following steps are specifically included：
1) raw material prepares, and weighs isometric base liquor A and base liquor B；Dendrobium candidum original powder is weighed, it is spare；
2) it concentrates, the dendrobium candidum original powder is added in the base liquor A, uniform stirring makes dendrobium candidum original powder slowly change
It opens and is dissolved in base liquor A, at least 83% dendrobium candidum original powder is concentrated on water-bath and is dissolved in the base liquor A, obtains iron
Skin stem of noble dendrobium concentrate；
3) it digests, amylase is added in the dendrobium candidum concentrate, water-bath hydrolyzes under the conditions of 50-60 DEG C, and holds
Continuous to be stirred, retention time 2-4 hour obtains dendrobium candidum enzymolysis liquid；
4) acidolysis, by the dendrobium candidum enzymolysis liquid and the mixed in hydrochloric acid of isometric ratio, and the recirculation water in boiling water bath
Solution at least 3 hours, obtains acid hydrolysis solution；
5) it neutralizes, PH to 6~7 is adjusted to the acid hydrolysis solution using sodium hydroxide solution, obtains dendrobium candidum neutralizer；
6) it filters, the dendrobium candidum neutralizer is let cool to 20-23 DEG C of room temperature, with centrifuge, obtains supernatant
Liquid；
7) it allocates, the supernatant is taken to be mixed to get Dendrobium officinale alcohol first product according to a certain percentage, then pass through with the base liquor B
Cross canned i.e. described Dendrobium officinale alcohol.
Preferably, in the preparation method of the Dendrobium officinale alcohol, each liter of base liquor A described in the step 2) is extremely
300 grams of dendrobium candidum original powder is added in less.
Preferably, in the preparation method of the Dendrobium officinale alcohol, the amylase in the step 3) is heat safe
Alpha-amylase, the dosage of the alpha-amylase are 1 with the mass ratio of dendrobium candidum original powder:3.
Preferably, in the preparation method of the Dendrobium officinale alcohol, supernatant is with the base liquor B's in the step 7)
Ratio 1:33-50.
A kind of Dendrobium officinale alcohol, wherein, dendrobium candidum original powder 12-18g is contained in every liter of Dendrobium officinale alcohol.
Advantages of the present invention：
1st, using hydrochloric acid carry out acidolysis reuse sodium hydroxide neutralize the acidolysis that can not only be effectively accomplished and in
The purpose of sum, product are sodium chloride, will not introduce new impurity and influence the mouthfeel of Dendrobium officinale alcohol.
2nd, alcoholic strength 45-50 pure rice wines or the pure liquor made from sorghums of alcoholic strength 50-55 can be selected to make according to the difference of local demand
For base liquor, north and south material conditions can not only be made full use of, while meet the diet difference in north and south, are more advantageous to industrial metaplasia
Production.
3rd, it using the fresh item of dendrobium candidum in 2 to April, using the peak time of the active principle content of dendrobium candidum, and passes through
Crossing drying or freeze-drying and then being crushed to the powder that mesh number is 400-500 preferably to adopt the effective active composition of dendrobium candidum
Collection is fused in wine, plays its effect.
4th, significantly more efficient dendrobium candidum macromolecular polysaccharide can be degraded into small molecule by the enzymolysis of alpha-amylase more
Sugar solves the problems, such as the alcohol-soluble of Dendrobium officinale polysaccharide along with the acidolysis of hydrochloric acid so that dendrobium candidum effective active composition
Activity can give full play to, and merge in wine so that people can absorb when drinking, and then achieve the purpose that health care.
5th, supernatant and base liquor are allocated according to a certain percentage, it can not only be effective in Dendrobium officinale alcohol is ensured
Active constituent meet the needs of health care also be able to simultaneously save wine brewing property, suitable for industrialization mass produce.
Specific embodiment
The present invention is described in further detail below, to enable those skilled in the art being capable of evidence with reference to specification word
To implement.
Embodiment 1
A kind of Dendrobium officinale alcohol, using dendrobium candidum original powder as base-material, using the pure rice wine that alcoholic strength is 50 degree as base liquor, through work
Skill is brewed to be formed.Wherein, dendrobium candidum original powder is mature dendrobium candidum stem item next year in 2 to April of picking, by drying
Then the mesh number for being crushed to cell is spare for 500 mesh.
A kind of brewing method of Dendrobium officinale alcohol, includes the following steps：
Step 1 takes 2 parts of base liquor, base liquor A and base liquor B, each 1000 milliliters；Weigh 1 part, 300 grams of dendrobium candidum original powder.
Dendrobium candidum original powder is added in base liquor A by step 2, and uniform stirring makes dendrobium candidum original powder slowly melt to be dissolved in base
In wine A, at least 250 grams of dendrobium candidum original powder is concentrated on water-bath and is dissolved in base liquor A, obtain dendrobium candidum concentration
Liquid.
Step 3 adds in 84 grams of alpha-amylases in dendrobium candidum concentrate, is persistently stirred under the conditions of 60 DEG C, water
Bath water solution 2 hours, obtains dendrobium candidum enzymolysis liquid.
Step 4, addition and the hydrochloric acid of dendrobium candidum enzymolysis liquid equal proportion, back hydrolysis 3 hours, obtain iron in boiling water bath
Skin stem of noble dendrobium acid hydrolysis solution.
Step 5 adds sodium hydroxide solution, and the PH of solution is adjusted to 7, obtains dendrobium candidum neutralizer.
Step 6 lets cool dendrobium candidum neutralizer to 20 DEG C of room temperature, then with centrifuge, obtains dendrobium candidum
Supernatant.
Step 7 takes 20 milliliters of dendrobium candidum supernatant to add in base liquor B, mixing up to embodiment 1 Dendrobium officinale alcohol.
Embodiment 2
A kind of Dendrobium officinale alcohol, using dendrobium candidum original powder as base-material, the pure liquor made from sorghum for being 50 degree using alcoholic strength is base liquor, warp
Technique is brewed to be formed.Remaining parameter is identical with embodiment 1, and infusion technology is also identical.
Embodiment 3
The step 3 of embodiment 1 is replaced with：84 grams of alpha-amylases are added in dendrobium candidum concentrate, in 60 DEG C of conditions
Under be persistently stirred, water-bath hydrolyze 3 hours, obtain dendrobium candidum enzymolysis liquid.Remaining parameter with 1 complete phase of embodiment
Together, infusion technology is also identical.
Embodiment 4
The step 3 of embodiment 1 is replaced with：84 grams of alpha-amylases are added in dendrobium candidum concentrate, in 60 DEG C of conditions
Under be persistently stirred, water-bath hydrolyze 4 hours, obtain dendrobium candidum enzymolysis liquid.Remaining parameter with 1 complete phase of embodiment
Together, infusion technology is also identical.
Embodiment 5
The step 7 of embodiment 1 is replaced with：30 milliliters of dendrobium candidum supernatant is taken to add in base liquor B, mixing is up to iron sheet
Dendrobium wine.Remaining parameter is identical with embodiment 1, and infusion technology is also identical.
Comparative example 1
Step 1 weighs 200 grams of dendrobium candidum, cleans and cuts into pieces, for use.
The dendrobium candidum of slice is put into wine jar by step 2, adds in 5 kilograms of the white wine of 60 degree or so grain brewings；30
It is extracted 72 hours under the conditions of degree.
Step 3, the stirring in every 24 hours of the medicinal material in immersion and wine product is primary, stirring five times altogether.
Step 4, the mixture after immersion is filtered to take leaching liquor, and it can be served.
Comparative example 2
Step 1 chooses the fresh stem of imitative dendrobium officinale, washes spare to the greatest extent；
Step 2, by edible enzyme stoste and pure water according to 1：800 ratio is uniformly mixed, the imitative open country that step 1 is chosen
The raw fresh stem of dendrobium candidum, which is put into aqueous enzyme, to be impregnated 50 minutes, is then picked up, is eluriated with pure water, then from dry machine from dry iron sheet stone
Dry measure used in former times surface moisture, it is spare；
Step 3 will be cut into through step 2 treated dendrobium candidum moss stem with pelleter granular, will prepare the iron that granulates
Skin stem of noble dendrobium moss stem is added in 40 degree of liquor made from sorghum, and being stirred evenly using rotating speed as 50 revs/min therebetween is reloaded into ware
Constant temperature storehouse is deposited in tool sealing, and storehouse maintains the temperature at 10 degrees Celsius to 30 degrees Celsius, impregnates 20 days；
The mixture of step 4, the dendrobium candidum stem grain that will impregnate 20 days and grain wine is packed into rustless steel container sealing and is stored in
Freezer is cooled to -15 DEG C in 24 hours；
Step 5, using power wall-breaking machine, in the pressure dendrobium candidum stem grain cooled for high speed wall-breaking abstraction under 130MPa
With the mixture of grain wine, processing time is 1 minute, makes mixture into sauce, and during broken wall under conditions of closing is protected from light
It runs, the cell-wall breaking ratio in mixture is 85%.
Broken wall is packed into ceramic vessel hermetically storing in constant temperature storehouse by step 6 into the dendrobium candidum of sauce and the mixture of wine
90 days, storehouse was maintained at temperature at 10 DEG C to 30 DEG C, dendrobium candidum active ingredient is allowed fully to be merged with wine, precipitation removes slag；
Step 7, removed slag with precipitation after Dendrobium officinale alcohol be packed into ceramic vessel and be stored in constant temperature cellar and obtain finished product in 90 days
Dendrobium officinale alcohol.
The imitative dendrobium officinale is the imitative dendrobium officinale obtained on attached phytosaur eye or litchi, this imitates wild iron
It is 42.05% that skin stem of noble dendrobium active polysaccharide, which contains, and the content of dendrobine is 0.014%, and total amino acid content is 20.32%.
Comparative example 3
Step 3 in embodiment 1 is replaced with：50 grams of alpha-amylases is taken to add in dendrobium candidum concentrate, remaining ginseng
Number is identical with embodiment 1, and infusion technology is also identical.
Comparative example 4
Step 3 in embodiment 1 is replaced with：100 grams of alpha-amylases is taken to add in dendrobium candidum concentrate, remaining ginseng
Number is identical with embodiment 1, and infusion technology is also identical.
Comparative example 5
The step 7 of embodiment 1 is replaced with：10 milliliters of dendrobium candidum supernatant is taken to add in base liquor B, mixing, remaining
Parameter is identical with embodiment 1, and infusion technology is also identical.
Product testing
It is next brewed with parameter and method cited in embodiment and comparative example, GB/ is passed through to obtained Dendrobium officinale alcohol
T27588-2011 detection methods, are detected.
Wherein, the Dendrobium officinale alcohol appearance obtained by embodiment 1-5 is limpid transparent, deposit-free and suspended matter；With plant
Fragrant and aroma, and it is all fragrant harmonious；Through trial test mouthfeel alcohol and, relax along harmony, wine body is complete；The dendrobium candidum of 1 gained of comparative example
Wine turbid appearance has layering to have sediment, has aroma, and slightly strong by tasting mouthfeel, wine body is complete；The iron of 2 gained of comparative example
Skin dendrobium wine appearance slightly muddiness has suspended matter, has plant perfume and aroma, and all fragrant harmonious；By tasting mouthfeel alcohol and relaxing
Along harmony, wine body is complete；Dendrobium officinale alcohol appearance obtained by comparative example 3-5 is limpid transparent, deposit-free and suspended matter；With plant
Object perfume and aroma, and it is all fragrant harmonious；Through trial test mouthfeel alcohol and, relax along harmony, wine body is complete.
It is next brewed with parameter and method cited in embodiment and comparative example, GB/ is passed through to obtained Dendrobium officinale alcohol
T10345-2007 detection alcoholic strength (20 DEG C), GB/T15038-2006 detection total acid content (with Acetometer), total sugar content (with
Glucose meter) and sugar-free extract content, GB/T27588-2011 detection total ester contents (in terms of ethyl acetate), GB/T5009.48-
2003 detection methanol contents, as a result such as table 1：
From table 1, it can be seen that as a result of the brewing method of Dendrobium officinale alcohol disclosed in this invention, gained in example
To Dendrobium officinale alcohol in contained active component content it is all very high, alcoholic strength is stablized, and maintains 45.0-46.0 degree；Total acid
Content reaches 0.18-0.36g/L (with Acetometer)；Total sugar content reaches 1.56-2.20g/L (with glucose meter)；Total ester content
(being counted using ethyl acetate) reaches 0.30-0.52g/L sugar-free extracts content as 2.31-2.51g/L；And do not detect methanol, safety
Reliably.
Also, the time control digested in embodiment is in the range of 2-4 hours, you can ensures fully will by enzymolysis
Dendrobium candidum macromolecular polysaccharide is degraded into micromolecular polysaccharide.The mass ratio of alpha-amylase and dendrobium candidum original powder is at least 1:3, with
1:3 be best, if it does, can not further continue to degrade, and the part alpha-amylase more added will be wasted.On
The ratio of clear liquid and the base liquor B are controlled 1:In the range of 33-50, if the ratio of taken supernatant is too low, after allotment
Wine kind in active component content will be less, the purpose of health care is not achieved；If the ratio of taken supernatant is excessively high, warp
Although the active component content crossed in the wine kind after allotment can be more, mouthfeel can be influenced, and nutrient is excessively also unfavorable for
It absorbs, while increases cost, a small amount of Precipitation is also had after being long placed in, causes to waste, is unfavorable for industrializing.
Compare with comparative example 1, comparative example 1 is using traditional Dendrobium officinale alcohol brewing method, and embodiment then uses
The brewing method of Dendrobium officinale alcohol disclosed in this invention, contained active component content in obtained Dendrobium officinale alcohol
All it is significantly larger than traditional brewing method, significantly more efficient can achievees the purpose that health liquor.
Compare with comparative example 2, employ the brewing method of Dendrobium officinale alcohol disclosed in this invention, obtained iron sheet stone
Contained active component content is above method used by comparative example 2, and brewing side of the present invention in dry measure used in former times wine
Method is all far superior to the method for comparative example 2 from time cost, equipment cost and operating cost, and it is extensive to be more suitable for industrialization
Production.
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With.It can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification.
Claims (9)
1. a kind of preparation method of Dendrobium officinale alcohol, it is characterised in that：After dendrobium candidum original powder is mixed with base liquor, through overrich
Contracting, amylase are digested, acidolysis, after neutralization and filtering, then allocate with base liquor to obtain the Dendrobium officinale alcohol.
2. the preparation method of Dendrobium officinale alcohol as described in claim 1, it is characterised in that：The acidolysis is carried out using hydrochloric acid,
The neutralization is carried out using sodium hydroxide.
3. the preparation method of Dendrobium officinale alcohol as described in claim 1, which is characterized in that the base liquor is alcoholic strength 45-50
Pure rice wine or the pure liquor made from sorghums of alcoholic strength 50-55.
4. the preparation method of Dendrobium officinale alcohol as described in claim 1, it is characterised in that：2 months to April is mature
Dendrobium candidum stem item next year, after picking through drying or be lyophilized and then be crushed to the powder that mesh number is 400-500 obtain it is described
Dendrobium candidum original powder.
5. the preparation method of Dendrobium officinale alcohol as described in claim 1, which is characterized in that specifically include following steps：
1) raw material prepares, and weighs isometric base liquor A and base liquor B；Dendrobium candidum original powder is weighed, it is spare；
2) it concentrates, the dendrobium candidum original powder is added in the base liquor A, it is molten that uniform stirring makes dendrobium candidum original powder slowly melt
It in base liquor A, is heated on water-bath, until at least 83% dendrobium candidum original powder is dissolved in the base liquor A, obtains iron sheet
Stem of noble dendrobium concentrate A；
3) digest, add in amylase in the dendrobium candidum concentrate A, under the conditions of 50-60 DEG C water-bath hydrolyze, and continue into
Row stirring, retention time 2-4 hour obtain dendrobium candidum concentrate B；
4) acidolysis, by the dendrobium candidum concentrate B and the mixed in hydrochloric acid of isometric ratio, and in boiling water bath, back hydrolysis is extremely
It is 3 hours few, obtain acid hydrolysis solution；
5) it neutralizes, pH to 6~7 is adjusted to the acid hydrolysis solution using sodium hydroxide solution, obtains neutralizer；
6) it filters, the neutralizer is let cool to 20-23 DEG C of room temperature, with centrifuge, obtains supernatant；
7) it allocates, the supernatant is taken to be mixed to get Dendrobium officinale alcohol first product according to a certain percentage with the base liquor B, using tank
Dress obtains the Dendrobium officinale alcohol.
6. the preparation method of Dendrobium officinale alcohol as claimed in claim 5, it is characterised in that：It is each described in the step 2)
Rise the dendrobium candidum original powder that base liquor A at least adds in 300 grams.
7. the preparation method of Dendrobium officinale alcohol as claimed in claim 5, it is characterised in that：Amylase in the step 3) is
The mass ratio of high-temperature resistant alpha-amylase, the alpha-amylase and dendrobium candidum original powder is 1:3.
8. the preparation method of Dendrobium officinale alcohol as claimed in claim 5, it is characterised in that：Supernatant and institute in the step 7)
State the ratio 1 of base liquor B:33-50.
9. a kind of Dendrobium officinale alcohol is made, which is characterized in that every liter using the preparation method as described in claim 1-8 is any
Contain dendrobium candidum original powder 12-18g in Dendrobium officinale alcohol.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108635300A (en) * | 2018-07-19 | 2018-10-12 | 南京润斛堂生物科技有限公司 | A kind of cosmetics containing small molecule dendrobium polysaccharide and preparation method and application |
| CN110484414A (en) * | 2019-07-16 | 2019-11-22 | 韶关学院 | It is a kind of using dendrobium nobile alcohol extracting thing as dendrobium wine of raw material and preparation method thereof |
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| CN106544256A (en) * | 2016-12-22 | 2017-03-29 | 西南林业大学 | A kind of dendrobium officinale health wine and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108635300A (en) * | 2018-07-19 | 2018-10-12 | 南京润斛堂生物科技有限公司 | A kind of cosmetics containing small molecule dendrobium polysaccharide and preparation method and application |
| CN110484414A (en) * | 2019-07-16 | 2019-11-22 | 韶关学院 | It is a kind of using dendrobium nobile alcohol extracting thing as dendrobium wine of raw material and preparation method thereof |
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Application publication date: 20180622 |