CN108185227A - A kind of feed of carp kidney cell and preparation method thereof - Google Patents
A kind of feed of carp kidney cell and preparation method thereof Download PDFInfo
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- CN108185227A CN108185227A CN201810194632.4A CN201810194632A CN108185227A CN 108185227 A CN108185227 A CN 108185227A CN 201810194632 A CN201810194632 A CN 201810194632A CN 108185227 A CN108185227 A CN 108185227A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of feeds of carp kidney cell and preparation method thereof, and the feed of carp kidney cell is counted in parts by weight, including following components：15 20 parts of fish meal, 35 45 parts of black soldier flies larva, 18 25 parts of wheat bran, 10 15 parts of tomato, 18 25 parts of corn, 5 10 parts of Hericium erinaceus, 8 15 parts of asparagus, 38 parts of garlic, 5 10 parts of black fungus, 37 parts of multi-vitamins, 5 10 parts of silkworm chrysalis, 38 parts of yeast, 15 25 parts of medical stone, 15 parts of cordate houttuynia, 0.5 1.5 parts of minerals.Step is as follows：(1) black soldier flies larva powder, dried silkworm chrysalis meal are prepared；(2) tomato is crushed, corn, Hericium erinaceus, asparagus, garlic, black fungus ferments after cordate houttuynia, fermented slurry processed；(3) black soldier flies larva powder, dried silkworm chrysalis meal, fermented slurry, fish meal, medical stone, minerals mixing granulation；(4) sterilization packaging.The feed can improve the immunity of carp kidney cell.
Description
Technical field
The present invention relates to aquatic feeds technical fields, and in particular to a kind of carp feed and preparation method thereof.
Background technology
Carp has and claims carp cripple, is under the jurisdiction of Cypriniformes, Cyprinidae, belongs to bottom and dwells ominivore-fish, meat and vegetables and food.Bait spectrum is wide
General, kiss bone is flourishing, often encircles mud and ingests, be the traditional improved seeds of China's freshwater aquiculture, has abundant practical value and nutrition
Value.
Existing carp feed generally with wheat bran, soya-bean cake, barley, fish meal, addition multi-vitamins, inorganic salts, lysine,
Methionine is formulated the needs to meet the nutriments such as the protein of carp in process of production, aliphatic acid.
But lack the research to the feed of carp kidney cell, due to developments such as the physiology of carp kidney cell and immune systems
Not enough completely, therefore in the case where the feed not matched is protected, it is easy to so that carp kidney cell is caught an illness, so as to make
It is low into juvenile fish survival rate, so, a kind of immunity for improving carp kidney cell is provided, the disease rate of carp kidney cell is reduced, is current
Urgent problem to be solved.
Invention content
The present invention for it is above-mentioned how carp kidney cell physiology and the ateliosis such as immune system and be easy to cause carp
The problem of juvenile fish catches an illness, provides a kind of feed of carp kidney cell and preparation method thereof, which can enhance the lymph T of juvenile fish
Cell, phagocyte and immunoglobulin, while antibacterial can be inhibited, so as to improve the immunity of carp kidney cell, so as to subtract
The disease rate of few carp kidney cell.
The technical solution adopted by the present invention is as follows：
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal
15-20 parts, 35-45 parts of black soldier flies larva, 18-25 parts of wheat bran, 10-15 parts of tomato, 18-25 parts of corn, 5-10 parts of Hericium erinaceus,
8-15 parts of asparagus, 3-8 parts of garlic, 5-10 parts of black fungus, 3-7 parts of multi-vitamins, 5-10 parts of silkworm chrysalis, 3-8 parts of yeast, medical stone
15-25 parts, 1-5 parts of cordate houttuynia, 0.5-1.5 parts of minerals.
Black soldier flies larva is used to reduce fish as the main protein of carp kidney cell and the source of aliphatic acid and raise in the present invention
The use of fish meal, not only economical and practical in material, but also improves the nutritive value of fish meal, in addition, black soldier flies larva is as elder brother
Worm has the antibacterial peptide components that solely contain of insect active proteins, can fast track, the invasion of chase pathogenic microorganism, and bacterium,
Ion channel is dug out in the after birth structure of virus, destroys bacterial membrane structure, intracellular water-soluble substances is caused to outflow, so as to
Thoroughly kill bacterium；Meanwhile insect active proteins can adjust pH value in animal immune system, adjust lotus rich in chitin
You cover and carry out two-way immunological regulation, and can also discharge cell and body fluid harmful substance, heavy metal ion and toxin；Together
When, insect protein contains a kind of alexin of small molecular protein, can repair immune system, at the same time, insect active proteins
Also containing ectogenous agglutinine, cell is promoted to be mutually bonded and inhibit its proliferation, the richer activity of normal cell can not only be made, and can kill
Go out mutant, resists virus spread, activates immunity.Therefore, using black soldier flies larva as one of main component of feed, energy
Enough improve the immunity of carp kidney cell.
In addition, also added with tomato in the carp kidney cell feed of the present invention, it, can due to containing tomatidine in tomato
Growth is inhibited bacteria, so as to reduce the invasion of pathogen, while containing abundant vitamin in tomato, so as in supplement feed
Vitamin；Hericium erinaceus and asparagus can improve the immunocompetence of T lymphocytes；Black fungus can enhance immunoglobulin group
It knits, increases antibody, garlic has the function of antibacterial well and sterilization, while can activate phagocyte, improves immunity, this
Outside, the addition of cordate houttuynia can play the ability inhibited bacteria with germ, so as to reduce the invasion of pathogen, while can also increase
The phagocytic activity of phagocyte, so as to play the role of improving immunity.In addition, medical stone is rich in abundant macroelement and micro-
Secondary element, while physiological function and bidirectional modulation acid-base value can be improved, so as to reduce the disease rate of carp kidney cell, in addition, wheat
Meal stone also is able to absorption harmful substance and pernicious gas, so as to extend the time that feed is detained in alimentary canal, so as to make nutrient
It is fully absorbed and used in alimentary canal, improves digestibility and utilization rate of the carp kidney cell to feed.And silkworm chrysalis not only contains
Abundant protein and unrighted acid, but also be capable of providing by leucocyte level, so as to improve immune function.Therefore,
The present invention is by black soldier flies larva, silkworm chrysalis, tomato, asparagus, Hericium erinaceus, cordate houttuynia, under the collective effect of garlic and black fungus,
It can not only inhibit even to kill germ and bacterium, prevent the intrusion of pathogen, meanwhile, it is capable to effectively adjust immune system, carry
The immunocompetence of high T lymphocytes enhances immunoglobulin tissue, increases antibody, while activate phagocyte, and it is thin to increase phagocytosis
The modes such as the phagocytic activity of born of the same parents improve the immunity of carp kidney cell, so as to reduce the disease rate of carp kidney cell from every side.This
Outside, the feed nutrition of carp kidney cell of the invention is balanced, and protein abundance can quickly supplement carp kidney cell in developmental process
Required energy, and also be able to improve digestibility and utilization rate of the carp kidney cell to feed so that carp Fast Growth.
Preferably, the feed of the carp kidney cell is counted in parts by weight, including following components：18-20 parts of fish meal, black soldier flies
38-43 parts of larva, 20-23 parts of wheat bran, 12-13 parts of tomato, 20-23 parts of corn, 8-10 parts of Hericium erinaceus, 10-12 parts of asparagus, greatly
5-7 parts of garlic, 8-10 parts of black fungus, 4-6 parts of multi-vitamins, 6-8 parts of silkworm chrysalis, 5-7 parts of yeast, 18-22 parts of medical stone, cordate houttuynia
1-3 parts, 0.8-1.2 parts of minerals.
Preferably, the feed of the carp kidney cell is counted in parts by weight, including following components：20 parts of fish meal, black soldier flies children
40 parts of worm, 20 parts of wheat bran, 12 parts of tomato, 20-23 parts of corn, 9 parts of Hericium erinaceus, 10 parts of asparagus, 6 parts of garlic, 10 parts of black fungus,
5 parts of multi-vitamins, 7 parts of silkworm chrysalis, 6 parts of yeast, 20 parts of medical stone, 2 parts of cordate houttuynia, 1 part of minerals.
Preferably, the multi-vitamins include：Vitamin A, vitamin C, vitamin D, vitamin B1, vitamin B2,
Vitamin B3, vitamin B6, vitamin B7, any one or a few in vitamin B12 are mixed above.
Preferably, the minerals include ferrous sulfate heptahydrate, cupric sulfate pentahydrate, white vitriol, manganese sulfate monohydrate, sulphur
Sour magnesium, sodium chloride, hydrogen sulfate dipotassium, any one or a few in dicalcium phosphate are mixed above.
Preferably, the preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, starched
Material adds in yeast and is uniformly mixed, seals 2-3 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, be squeezed under conditions of steam regulation of texture, conditioning of texture, 90-100 DEG C of material temperature
Grain is then dried, and feed granules sample is made in cooling；
(4) feed granules sample prepared by step (3) is gone out in temperature for the carry out high-temperature steam under the conditions of 130-135 DEG C
Bacterium, sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. the present invention is by black soldier flies larva, silkworm chrysalis, tomato, asparagus, Hericium erinaceus, cordate houttuynia, garlic and black fungus
Under collective effect, it can not only inhibit even to kill germ and bacterium, the intrusion of pathogen be prevented, meanwhile, it is capable to effectively adjust
Immune system improves the immunocompetence of T lymphocytes, enhances immunoglobulin tissue, increases antibody, while activates phagocytosis thin
Born of the same parents increase the modes such as the phagocytic activity of phagocyte, the immunity of carp kidney cell are improved from every side, so as to reduce carp kidney cell
Disease rate.
2. the feed nutrition of the carp kidney cell of the present invention is balanced, protein abundance, can quickly supplement carp kidney cell into
Required energy in growth process, and also be able to improve digestibility and utilization rate of the carp kidney cell to feed so that carp is quick
Growth.
Specific embodiment
In order to which those skilled in the art is made to more fully understand technical scheme of the present invention, with reference to specific embodiment pair
The present invention is described in further detail.
In the inventive solutions, minerals use ferrous sulfate heptahydrate, cupric sulfate pentahydrate, white vitriol, one
Water manganese sulfate, magnesium sulfate, sodium chloride, hydrogen sulfate dipotassium, any one or a few in dicalcium phosphate are mixed above.Wherein seven
Aqueous ferrous sulfate supplements animal body ferro element, manganese sulfate monohydrate supplement animal body manganese element, sodium chloride, hydrogen sulfate dipotassium, phosphoric acid
Hydrogen dicalcium supplements animal body sodium element, potassium element and calcium constituent respectively.Multi-vitamins use vitamin A, vitamin C, dimension life
Plain D, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B7, any one or a few in vitamin B12
It is mixed above.Vitamin A, vitamin C, vitamin D, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin
B7, vitamin B12 supplement the required vitamin A of animal body respectively, vitamin C, vitamin D, vitamin B1, vitamin B2,
Vitamin B3, vitamin B6, vitamin B7, vitamin B12.Therefore, it is suitable to be selected according to the needs of the following example
Minerals and multi-vitamins.
Embodiment 1
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal 15
Part, 35 parts of black soldier flies larva, 18 parts of wheat bran, 10 parts of tomato, 18 parts of corn, 5 parts of Hericium erinaceus, 8 parts of asparagus, 3 parts of garlic, black wood
5 parts of ear, 3 parts of multi-vitamins, 5 parts of silkworm chrysalis, 3 parts of yeast, 15 parts of medical stone, 1 part of cordate houttuynia, 0.5 part of minerals.
The preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, starched
Material adds in yeast and is uniformly mixed, seals 3 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 100 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 135 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Embodiment 2
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal 20
Part, 45 parts of black soldier flies larva, 25 parts of wheat bran, 15 parts of tomato, 25 parts of corn, 10 parts of Hericium erinaceus, 15 parts of asparagus, 8 parts of garlic are black
10 parts of agaric, 7 parts of multi-vitamins, 10 parts of silkworm chrysalis, 8 parts of yeast, 25 parts of medical stone, 5 parts of cordate houttuynia, 1.5 parts of minerals.
The preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, starched
Material adds in yeast and is uniformly mixed, seals 2 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 90 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 130 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Embodiment 3
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal 18
Part, 38 parts of black soldier flies larva, 20 parts of wheat bran, 12 parts of tomato, 20 parts of corn, 8 parts of Hericium erinaceus, 10 parts of asparagus, 5 parts of garlic are black
8 parts of agaric, 4 parts of multi-vitamins, 6 parts of silkworm chrysalis, 5 parts of yeast, 18 parts of medical stone, 1 part of cordate houttuynia, 0.8 part of minerals.
The preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, is starched
Material adds in yeast and is uniformly mixed, seals 3 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 100 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 130 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Embodiment 4
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal 20
Part, 43 parts of black soldier flies larva, 23 parts of wheat bran, 13 parts of tomato, 23 parts of corn, 10 parts of Hericium erinaceus, 12 parts of asparagus, 7 parts of garlic are black
10 parts of agaric, 6 parts of multi-vitamins, 8 parts of silkworm chrysalis, 7 parts of yeast, 22 parts of medical stone, 3 parts of cordate houttuynia, 1.2 parts of minerals.
The preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, is starched
Material adds in yeast and is uniformly mixed, seals 2 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 90 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 135 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Embodiment 5
A kind of feed of carp kidney cell, the feed of the carp kidney cell is counted in parts by weight, including following components：Fish meal 20
Part, 40 parts of black soldier flies larva, 20 parts of wheat bran, 12 parts of tomato, 20-23 parts of corn, 9 parts of Hericium erinaceus, 10 parts of asparagus, 6 parts of garlic,
10 parts of black fungus, 5 parts of multi-vitamins, 7 parts of silkworm chrysalis, 6 parts of yeast, 20 parts of medical stone, 2 parts of cordate houttuynia, 1 part of minerals.
The preparation method of the feed of the carp kidney cell, includes the following steps：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, is starched
Material adds in yeast and is uniformly mixed, seals 3 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), then add
Enter fish meal, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 100 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 130 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Check experiment
Choose that 900 urosome types are uniform, and body colour is normal, and vitality is good, the similar carp of age in days is put down as experimental subjects
900 tail crucian juvenile fish are randomly divided into 6 groups by equal weight 4g or so, are respectively tested 1 group, 2 groups of experiment, are tested 3 groups, experiment 4
Group tests 5 groups and control group, respectively puts 6 groups of juvenile fish into identical ponds such as 6 water quality, landform, illumination, temperature in a suitable place to breed, point
Not detecting weight at 30 days, survival rate, Baiyun Disease disease pest rate and variola disease pest and disease damage rate, testing result is shown in Table 1, experimental stage,
1 group is tested using feed described in the embodiment of the present invention 1,2 groups of experiment is used described in the embodiment 2 without Chinese medicine additive
Feed, 3 groups of experiment use the feed described in embodiment 3 without Chinese medicine additive, and 4 groups of experiment is added using not having Chinese medicine
Feed described in the embodiment 4 of agent, 5 groups of feeds used described in the embodiment 5 without Chinese medicine additive of experiment, control group are adopted
The carp feed bought with common market, in feeding process, the number that feeds intake, inventory and charging time all same.It is cultivated
Effect is shown in Table 1.
1 carp kidney cell culture experiment result of table
| Project | Test 1 group | Test 2 groups | Test 3 groups | Test 4 groups | Test 5 groups | Control group |
| Average just weight (g) | 4.10 | 4.11 | 4.09 | 4.15 | 4.12 | 4.10 |
| Average opisthosoma weight (g) | 5.92 | 5.92 | 5.83 | 5.89 | 5.98 | 5.10 |
| Body weight growth rate (%) | 44.39 | 44.04 | 42.54 | 41.93 | 45.15 | 24.39 |
| Baiyun Disease disease pest rate (%) | 1 | 1.5 | 0.9 | 0.8 | 0.75 | 10 |
| Variola disease pest and disease damage rate (%) | 1.5 | 1.2 | 1 | 0.9 | 0.7 | 9 |
| Survival rate (%) | 93.5 | 94 | 94.5 | 92.5 | 95 | 80.1 |
Note：Body weight growth rate (%)=(average opisthosoma weight-average just weight)/average just weight * 100%；
There is upper table it is found that compared with common carp feed, the weight of the carp kidney cell of forage feed using the present invention increases
Long rate is up to 45.15%, and the body weight growth rate of the carp kidney cell of common carp forage feed only only has 24.39%, therefore,
Illustrate that the feed of the present invention can aid in the digestion of carp kidney cell, absorb, meet the battalion such as the required protein of carp kidney cell
Support substance.In addition, compared with common fish meal, the Baiyun Disease disease pest rate of the carp kidney cell of forage feed using the present invention down to
0.75%, variola disease pest and disease damage rate 0.7%, and survival rate is up to 95%, illustrating the fish meal of the present invention can effectively enhance
The immunity of carp kidney cell reduces the disease rate of carp kidney cell.
The above is the preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art,
Without departing from the technical principles of the invention, several improvement and modification can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of feed of carp kidney cell, which is characterized in that the feed of the carp kidney cell is counted in parts by weight, including with the following group
Point：15-20 parts of fish meal, 35-45 parts of black soldier flies larva, 18-25 parts of wheat bran, 10-15 parts of tomato, 18-25 parts of corn, Hericium erinaceus
5-10 parts, 8-15 parts of asparagus, 3-8 parts of garlic, 5-10 parts of black fungus, 3-7 parts of multi-vitamins, 5-10 parts of silkworm chrysalis, yeast 3-8
Part, 15-25 parts of medical stone, 1-5 parts of cordate houttuynia, 0.5-1.5 parts of minerals.
2. the feed of a kind of carp kidney cell according to claim 1, which is characterized in that the feed of the carp kidney cell is by weight
Number meter is measured, including following components：18-20 parts of fish meal, 38-43 parts of black soldier flies larva, 20-23 parts of wheat bran, tomato 12-13
Part, 20-23 parts of corn, 8-10 parts of Hericium erinaceus, 10-12 parts of asparagus, 5-7 parts of garlic, 8-10 parts of black fungus, multi-vitamins 4-6
Part, 6-8 parts of silkworm chrysalis, 5-7 parts of yeast, 18-22 parts of medical stone, 1-3 parts of cordate houttuynia, 0.8-1.2 parts of minerals.
3. the feed of a kind of carp kidney cell according to claim 2, which is characterized in that 20 parts of fish meal, black soldier flies larva 40
Part, 20 parts of wheat bran, 12 parts of tomato, 20-23 parts of corn, 9 parts of Hericium erinaceus, 10 parts of asparagus, 6 parts of garlic, 10 parts of black fungus are compound
5 parts of vitamin, 7 parts of silkworm chrysalis, 6 parts of yeast, 20 parts of medical stone, 2 parts of cordate houttuynia, 1 part of minerals.
4. the feed of a kind of carp kidney cell according to claim 1, which is characterized in that the multi-vitamins include：Dimension
Raw element A, vitamin C, vitamin D, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B7, vitamin B12
In any one or a few is mixed above.
5. the feed of a kind of carp kidney cell according to claim 1, which is characterized in that the minerals include seven water sulfuric acid
Ferrous iron, cupric sulfate pentahydrate, white vitriol, manganese sulfate monohydrate, magnesium sulfate, sodium chloride, hydrogen sulfate dipotassium, in dicalcium phosphate
Any one or a few is mixed above.
6. a kind of preparation method of the feed of the carp kidney cell described in claim 1-5 any one, which is characterized in that including with
Lower step：
(1) by black soldier flies larva, after silkworm chrysalis drying, black soldier flies larva powder and dried silkworm chrysalis meal are prepared into；
(2) by tomato, corn, Hericium erinaceus, asparagus, garlic, black fungus after cordate houttuynia crusher in crushing, obtains slurry, adds
Enter yeast to be uniformly mixed, seal 2-3 days, obtain fermented slurry；
(3) the black soldier flies larva powder and dried silkworm chrysalis meal for preparing step (1), fermented slurry mixing prepared by step (2), adds fish
Powder, medical stone, minerals mixing, it is to be mixed uniformly after, the extruding granulating under conditions of steam regulation of texture, conditioning of texture, 90-100 DEG C of material temperature, with
After dry, cool down, feed granules samples be made；
(4) the carry out high-temp steam sterilizing by feed granules sample prepared by step (3) under the conditions of temperature is 130-135 DEG C,
Sterilize 18-25min, then is dried, and packs, and feed granules finished product is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810194632.4A CN108185227A (en) | 2018-03-09 | 2018-03-09 | A kind of feed of carp kidney cell and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810194632.4A CN108185227A (en) | 2018-03-09 | 2018-03-09 | A kind of feed of carp kidney cell and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108185227A true CN108185227A (en) | 2018-06-22 |
Family
ID=62595399
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810194632.4A Pending CN108185227A (en) | 2018-03-09 | 2018-03-09 | A kind of feed of carp kidney cell and preparation method thereof |
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| Country | Link |
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| CN (1) | CN108185227A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850731A (en) * | 2018-08-30 | 2018-11-23 | 安徽金牧饲料有限公司 | A kind of carp feed being able to suppress water source biopathogen bacterium |
| RU2777768C1 (en) * | 2021-12-20 | 2022-08-09 | федеральное государственное бюджетное образовательное учреждение высшего образования "Донской государственный технический университет" (ДГТУ) | Trout feed |
-
2018
- 2018-03-09 CN CN201810194632.4A patent/CN108185227A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850731A (en) * | 2018-08-30 | 2018-11-23 | 安徽金牧饲料有限公司 | A kind of carp feed being able to suppress water source biopathogen bacterium |
| RU2777768C1 (en) * | 2021-12-20 | 2022-08-09 | федеральное государственное бюджетное образовательное учреждение высшего образования "Донской государственный технический университет" (ДГТУ) | Trout feed |
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Application publication date: 20180622 |