CN108185142A - A kind of diet for improving live pig digestive function and its application - Google Patents
A kind of diet for improving live pig digestive function and its application Download PDFInfo
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- CN108185142A CN108185142A CN201810192543.6A CN201810192543A CN108185142A CN 108185142 A CN108185142 A CN 108185142A CN 201810192543 A CN201810192543 A CN 201810192543A CN 108185142 A CN108185142 A CN 108185142A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of diet for improving live pig digestive function and its applications；It is to be combined the nutritional need of pig with idiotrophic substance digestion metabolic rhythm, set about from daily daily ration supply, the feeding diet in the morning and the feeding diet in afternoon are prepared, having been embodied in diet on diet net energy and lysine level daily morning and evening has apparent difference, realize the precision of diet, more to meet the nutritional need under pig own biological entrainment, pig net energy and amino acid difference alienation diet are formulated out in exploration.Experimental result is shown：The set diet is remarkably improved the feed digestibility of live pig, improves live pig digestive function.
Description
Technical field
The invention belongs to livestock feed Development Techniques field, more particularly to a kind of net energy for improving live pig digestive function and
The Feed formulation method of amino acid difference alienation.
Background technology
Biological rhythm（circadian thythms）, it is the active that organism is cooked the living environment periodically occurred
Adaptation reaction can influence all physiological behaviors, sleep/awakening and fasted/fed cycle, internal system, body temperature, enteron aisle
Physiology, liver metabolism etc..The molecular basis of biological rhythm is by some Circadian Genes（Clock, Bmall, Cry1,
Cry2, Per1, Per2, Per3, Rev-erba etc.）Transcription and the molecular oscillation that is formed of post-transcriptional control, rhythm and pace of moving things base
After startup, corresponding protein is generated through transcription and translation, after protein concentration reaches to a certain degree, feedback effect is in itself base
Because of promotor, its concentration is made to be vibrated with period self-excitation for 24 hours.
Positive effect is had to the growth metabolism of animal based on biological rhythm theory feeding different level nutriment.For this
Mechanism Study caused by kind positive effect is not yet reported.But have been reported that display, biological rhythm is to different metabolism
The direct regulation and control effect of journey is mainly by regulating and controlling the various signaling molecules generated in metabolic process, mammal peripheral
The gene for having 5%-10% in tissue such as liver, skeletal muscle, adipose tissue is all expressed in the rhythm and pace of moving things, and with apparent organizing specific
Property；With being metabolized relevant hormone, such as insulin, glucagon, adiponectin, Adrenal Glucocorticoid, leptin and gastrin
It is all secreted in rhythmicity, illustrates that they are all controlled by biological rhythm；In addition, biological rhythm can also be by adjusting metabolizing enzyme
Expression or its activity and cholesterol metabolic, amino acid metabolism, drug and toxin metabolism, tricarboxylic acid cycle, hepatic glycogen and Portugal
The activity of grape glycometabolism correlation movement system adjusts metabolism and the energy balance in liver and other peripheral tissues, wherein wrapping
Include liver glycogen phosphorylase, cytochrome oxidase, lactic dehydrogenase, acetyl-CoA carboxylase etc..On rodent
The study found that alimentary canal includes functional living being clock gene, these gene expressions of mouse are in myenteron, as in digestive system
The nervous system in portion, influences the function and activity of gastrointestinal tract, for example, in gastric emptying, gastric acid secretion, small intestine nutriment transport and
Proliferation of epithelial cell etc.；The nutriment transport protein that limit is raised in animal body still has certain rhythmicity, this illustrates biological section
It restrains in the effect that transport protein is adjusted in intestinal wall and plays important role.
NRC（1998）Point out there is most suitable lysine and energy ratio for grower pigs, when daily ration amino acid levels and energy
The maximum growth potentiality of competence exertion pig when intake matches.Net energy is to weigh energy required for animal maintains and produces most
Good index accurately can make estimation, it was reported that raw using net energy system to the energy consumed in pig maintenance and production process
The long daily net energy for maintenance of pig（NEm）Requirement be 71kcal/kgBW0.75、78kcal/kgBW0.75、86kcal/kgBW0.75；Rely
Propylhomoserin is generally considered to be the base of the first limiting amino acids and ideal protein pattern in corn-soybean meal swine rations
Plinth, its growth to pig, the raising of feed conversion rate, utilization rate and carcass lean meat percentage have good action, and therefore, supply is suitable
The diet of lysine level, it is of crucial importance for pig production.
Addition ratio is rested on to the research that lysine in daily ration and net energy match to inquire into the static of pig physiological status,
And the organic system complicated as one, pig body is due to the regulation and control of biological rhythm, and daily different time sections are to nutrition in diet
The demand of substance is different, and therefore, this patent is based on the true nutritional need of live pig body, and initiative one kind can be provided for live pig
Accurately net energy and lysine diet.
Invention content
The nutritional need of pig is combined by this patent with idiotrophic substance digestion metabolic rhythm, is supplied from daily daily ration
Set about, should ensure the whole nutritional need for meeting piglet, shown in diet net energy daily morning and evening and lysine level again
Difference is shown, realizes the precision of diet, more to meet the nutritional need under pig own biological entrainment, pig is formulated out in exploration
Net energy and amino acid difference alienation diet.
Technology the present invention overcomes influence of the research diet nutrient level static, linear in animal nutrition to pig lacks
It falls into, a kind of dynamic net energy and the horizontal diet of effective lysine is provided.The purpose of the present invention is improve live pig digestive function.
To achieve the above object, the invention discloses following technology contents：
A kind of diet for improving live pig digestive function, it is characterised in that it is made of the raw material of following portions by weight：
（1）Morning diet；
（2）Afternoon diet；
The present invention further discloses the diets for improving live pig digestive function to improve feedstuff for live pigs digestibility, improves live pig digestion
Application in terms of function；The results show：Feed high energy+high effective lysine diet in the morning（Net energy 2350kcal/kg, have
Imitate lysine 0.95%）, afternoon feeding low energy+low effective lysine diet（Net energy 2290kcal/kg, effective lysine 0.85%）
The feed digestibility of live pig is remarkably improved, improves live pig digestive function.
The present invention mainly prepares different net energy-effective lysine concentration diet, then according to the true nutritional need of live pig,
Net energy-effective lysine concentration diets different with feeding in afternoon, ultimate analysis difference feeding pattern disappear to live pig in the morning respectively
Change the influence of function.
Net energy-effective lysine concentration diets different from feeding in afternoon of the invention in the morning of mainly having investigated digest live pig
The influence of function, emphasis are to improve live pig digestive function by adjusting diet net energy-effective lysine concentration, formulate novel net energy
With amino acid difference alienation diet.The difficult point of invention is the net energy for groping to meet live pig autodigestion metabolic rule-effectively bad ammonia
The nutritional need of acid is horizontal.The innovation of the invention consists in that with reference to body biological rhythm, provided for the daily different time sections of live pig
Different net energy-effective lysine concentration diet, make diet nutrient level closer to the nutritional need of live pig, formulate out pig net energy and
Amino acid difference alienation diet.
The detailed test method of the present invention and effect are as follows：
1st, experimental design
The pig 240 of 30 kilograms or so weight is chosen, is divided into 3 processing, 4 repetitions is each handled, each repeats 20 pigs.Examination
Test A group mornings 5:00 feeding high energy+high effective lysine diet（Net energy 2350kcal/kg, effective lysine 0.95%）, afternoon
15:00 feeding low energy+low effective lysine diet（Net energy 2290kcal/kg, effective lysine 0.85%）, experiment B groups are control
Group feeding Basic drawing（Net energy 2320kcal/kg, effective lysine 0.90%）, test C group mornings 5:00 feeding low energy+low has
Imitate lysine diet（Net energy 2290kcal/kg, effective lysine 0.85%）, afternoon 15:00 feeding high energy+high effective lysine
Diet（Net energy 2350kcal/kg, effective lysine 0.95%）；
Experiment B groups are the Basic drawings designed with reference to 30kg grower pigs nutritional need in NRC2012, and Basic drawing is with corn-beans
Based on the dregs of rice, experiment A groups pass through fixed lysine dosage with experiment C groups, to be formulated raw materials used proportioning carry out it is appropriate adjust with
Determine energy and effective lysine concentration in diet.By adding soya-bean oil in experiment A group diets, reduce corn in Basic drawing and use
Amount improves shelling dregs of beans dosage to improve net energy level, and experiment C groups reduce corn and shelling dregs of beans dosage, improve wheat bran simultaneously
Dosage is horizontal to reduce net energy.
2nd, sample collection：
At the 21st and 26 day of feeding experiment, pig excrement is collected with endogenous indicator method, 10% is added according to the 5% of excrement sample fresh weight
The concentrated sulfuric acid, and add in 2 drop toluene anti-corrosions, be placed in -20 DEG C of refrigerators and preserve；After receiving excrement, by the excrement sample mixing of each repetition,
Sampling 25%, dries in 65 DEG C of insulating boxs, is put into air 24 h that get damp again, weighs, and crushed 40 mesh sieve, and -20 DEG C of preservations are treated
It surveys.
3rd, test method：
Daily ration and argol substance, thick protein, crude fat, crude fibre, calcium and total phosphorus respectively refer to country of the People's Republic of China (PRC)
Standard GB/T 6435-1986, GB/T 6432-1994, GB/T 6433-1994, GB/T 6434-1994, GB/T 6436-
The method that 2002 and GB/T 6437-2002 recommend measures, and the total energy of daily ration and excrement will according to instrument with oxygen bomb formula calorimetric instrument 6400
Ask time-and-motion study.The measure of ash insoluble in hydrochloric acid (AIA) content is with reference to GB/T23742-2009《Ash insoluble in hydrochloric acid in feed
It measures》In method detection.Diet Nutrient apparent digestibility is calculated according to the following formula：
Certain Nutrient Apparent Digestibility (%)=(1-Z × AIAdiet/AIA × Zdiet) × 100.
In formula:Z and Zdiet represents the content of certain nutrient in excrement and diet respectively, AIA and AIA diet are represented respectively
The content of AIA in excrement and diet.
Daily ration and excrement sample pass through at 110 DEG C at 24 h and 0 DEG C of 6 mol/L hydrochloric acid hydrolysis after 16 h of performic oxidation respectively
Hydrochloric acid hydrolyzes 24 h automatic amino acid analyzers（Hitachi L-8900, Japan）Measure 17 kinds of amino acid.
4th, test result analysis：
The influence of 4.1 net energy and amino acid difference alienation diet to feed digestibility
As shown in Table 1, when feeding net energy and amino acid difference alienation diet to 21 days, compared with the control group, A groups and C group crude protein
Digestibility 7.09% and 4.90% has been respectively increased（P>0.05）, difference is not notable between A groups and C groups；Crude fat digestibility point
3.67% and 3.40% are not improved（P>0.05）, difference is not notable between A groups and C groups；Coarse ash digestibility is respectively increased
8.35%（P>0.05）With 4.02%（P<0.05）, difference is not notable between A groups and C groups；Phosphorus digestibility has been respectively increased 9.36%（P
>0.05）With 6.28（P<0.05）, difference is not notable between A groups and C groups；The digestibility difference of calcium is not notable between each processing group.
TableThe influence of net energy and amino acid difference alienation diet to feed nutrient digestibility（21d）
As shown in Table 2, when feeding net energy and amino acid difference alienation diet to 21 days, compared with the control group, A groups and C group crude fat
Digestibility you can well imagine compared with B component it is high by 3.67%（P>0.05）With 2.09%（P<0.05）, difference is not notable between A groups and C groups；Phosphorus
Digestibility is respectively increased 8.33%（P>0.05）With 1.09%（P<0.05）, difference is not notable between A groups and C groups；A groups are thick with C groups
The digestibility of albumen is improved, but difference is not notable；A group coarse ash digestibilities slightly improve, but difference is not notable；Calcium disappears
Rate A groups are low compared with B groups with C groups, and difference is not notable.
The influence of 2 net energy of table and amino acid difference alienation diet to feed nutrient digestibility（26d）
The influence of 4.2 net energy and amino acid difference alienation diet to feed amino acid digestibility
As shown in Table 3, when feeding net energy and amino acid difference alienation diet to 21 days, compared with the control group, A groups and day in C group excrement
Winter propylhomoserin, serine, glutamic acid, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, relies threonine
The digestibility of propylhomoserin and proline is significantly increased compared with B groups（P>0.05）, difference is not notable between A groups and C groups.A groups and C groups
Glycine, cysteine, histidine and arginine are improved compared with B groups in excrement, and A groups improve notable（P>0.05）, the raising of C groups is not
Significantly（P<0.05）.The Methionine Levels significant difference of three processing groups（P>0.05）.
The influence of 3 net energy of table and amino acid difference alienation diet to amino acid digestibility in excrement（21d）
As shown in Table 4, when feeding net energy and amino acid difference alienation diet to 26 days, A groups are with methionine digestibility in C group excrement compared with B
Group is respectively increased 9.10%（P>0.05）With 5.79%（P<0.05）, difference is not notable between A groups and C groups；Remaining detection amino acid
Digestibility respectively to handle group difference not notable.
The influence of 4 net energy of table and amino acid difference alienation diet to amino acid digestibility（26d）
Conclusion：
Morning feeding high energy+high effective lysine diet of this patent initiative（Net energy 2350kcal/kg, effective lysine
0.95%）, afternoon feeding low energy+low effective lysine diet（Net energy 2290kcal/kg, effective lysine 0.85%）It can significantly carry
The feed digestibility of high live pig improves live pig digestive function.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Range, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
The various changes carried out to the material component in these embodiments and dosage under the premise of invention spirit and scope or change
It belongs to the scope of protection of the present invention.The raw materials used in the present invention and reagent are commercially available；H102 premixs therein, purchased from great achievement Wanda
（Tianjin）Co., Ltd.
Embodiment 1
The diet of live pig digestive function can be improved by preparing, and following diet components are prepared according to the composition of parts by weight.
（1）Morning diet；
（2）Afternoon diet；
Morning diet/afternoon diet preparation method:
Parts by weight according to raw material each in formula are prepared, such as prepare the morning diet of 1000kg, can be incited somebody to action
701.2kg corns -14%, 19.8kg level Four soya-bean oil, 187.72kg shelling dregs of beans, 50kg wheat bran, 9.99kg calcium monohydrogen phosphates,
10.5kg mountain flours, 4.52kg refined salt, -99% powder of 1.42kg methionine, 4.5kg lysines 98.5%, 0.48kg tryptophans 98%,
1.87kg threonines 98.5%, 3kg acidulants and 5kg H102 are mixed, and in the morning 5：00 feeding；It prepares
The diet in afternoon of 1000kg, can be by 597.1kg corns -14%, 136.62kg shellings dregs of beans, 150kg wheat bran, 8.83kg phosphoric acid hydrogen
Calcium, 11.75kg mountain flours, 4.8kg refined salt, -99% powder of 1.15kg methionine, 4.5kg lysines 98.5%, 0.4kg tryptophans 98%,
1.85kg threonines 98.5%, 3kg acidulants, 5kg H102 premixs and 75kg rice brans mixed, and afternoon 15：00
Feeding, two kinds of diets store and live pig production are respectively applied in morning and afternoon respectively.
Claims (2)
1. a kind of diet for improving live pig digestive function, it is characterised in that it is made of the raw material of following portions by weight：
Morning diet；
（2）Afternoon diet；
。
2. the diet described in claim 1 for improving live pig digestive function is improving feedstuff for live pigs digestibility, improve live pig digestion
Application in terms of function.
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