CN108184873A - A kind of medicament composition of the prevention phytobacterial disease containing epsilon-polylysine - Google Patents
A kind of medicament composition of the prevention phytobacterial disease containing epsilon-polylysine Download PDFInfo
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- CN108184873A CN108184873A CN201810084338.8A CN201810084338A CN108184873A CN 108184873 A CN108184873 A CN 108184873A CN 201810084338 A CN201810084338 A CN 201810084338A CN 108184873 A CN108184873 A CN 108184873A
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- Prior art keywords
- polylysine
- medicament composition
- epsilon
- prevention
- natamycin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of medicament composition for preventing phytobacterial disease, active ingredient includes：ε polylysines, natamycin and filler.The medicament composition has the following advantages：It is notable that ε polylysines, natamycin etc. are mixed in a certain ratio rear bactericidal synergistic effect；Medicament composition diseases prevention spectrum is wide, has significant preventive and therapeutic action to the bacteriosis of a variety of host plants；The medicament composition can reduce the risk for using and fighting pharmacological property generation of chemical pesticide, preserve the ecological environment, meet the requirement of environment protective plant protecting and Ecological Civilization Construction.
Description
Technical field
The present invention relates to technical field of pesticide more particularly to a kind of medicines of the prevention phytobacterial disease containing epsilon-polylysine
Agent composition.
Background technology
Wide, weight of causing harm occurs for phytobacterial disease, and the pesticide variety for preventing such disease at present is less.
Epsilon-polylysine is novel food preservative, is produced by microbial metabolism, nontoxic, noresidue, is had bright
Aobvious bactericidal effect is widely used in the fields such as food, medicine, medical treatment and cosmetics, in the mycotic pesticide of crop is prevented
Auxiliary material is used as synergist and in fertilizer it has been reported that but having no application report in terms of phytobacterial disease is prevented.
The chemical structural formula of epsilon-polylysine is as follows：
Natamycin is a kind of polyene macrolide object for passing through submerged fermentation generation by streptomycete, is a kind of efficient, peace
Full New Biological Preservatives.It is nontoxic, noresidue, applied to food, medicine and other fields.Prevention food spoilage fungi,
And have part as medical antibacterial agent aspect and report, but application report is had no in terms of phytobacterial disease is prevented.Receive him
The chemical structural formula of mycin is as follows：
Epsilon-polylysine is applied to prevention phytobacterial disease technical field of pesticide, and press one with natamycin by the present invention
Certainty ratio mixing is used in conjunction with, better.
Invention content
Technical problems based on background technology, the present invention propose a kind of prevention vegetative bacteria containing epsilon-polylysine
The medicament composition of disease.
Technical scheme is as follows：
A kind of medicament composition of the prevention phytobacterial disease containing epsilon-polylysine, including following component：ε-poly- bad ammonia
Acid, natamycin and filler.
Preferably, the filler and auxiliary agent include：Diatomite, white carbon and sodium lignin sulfonate.
Preferably, the medicament composition can be processed into 1%~70% wettable powder.
Preferably, the phytobacterial disease can be the bacteriosis of the plants such as vegetables, fruit tree, crops, flowers
In any one, in bacterial blight of rice, bacterial wilt of tomato, soft rot of Chinese cabbage, angular leaf spot of cucumber, wildfire
Any one.
The invention has the beneficial effects that：By more than substance formulated in combination, has the following advantages：1. epsilon-polylysine is received
It is notable that his mycin is mixed in a certain ratio rear bactericidal synergistic effect.2. diseases prevention spectrum is wide, to the various bacteria of a variety of host plants
Disease has significant preventive and therapeutic effect.
Description of the drawings
Fig. 1 is wettable powder production technological process.
Specific embodiment
Embodiment 1
The medicament composition that a kind of prevention bacteriosis is fallen ill is wettable powder, prepares 100kg wettable powders, packet
Include following component：Epsilon-polylysine 2.5kg, natamycin 2.0kg, sodium lignin sulfonate 10.0kg, diatomite 45.0kg, hard charcoal
Black surplus.
Active constituent content accounts for 4.5% in the wettable powder.
Embodiment 2
The medicament composition that a kind of prevention bacteriosis is fallen ill is wettable powder, prepares 100kg wettable powders, packet
Include following component：Epsilon-polylysine 5.0kg, natamycin 4.5kg, sodium lignin sulfonate 8.0kg, diatomite 40.0kg, hard charcoal
Black surplus.
Active constituent content accounts for 9.5% in the wettable powder.
Embodiment 3
The medicament composition that a kind of prevention bacteriosis is fallen ill is wettable powder, prepares 100kg wettable powders, packet
Include following component：Epsilon-polylysine 15.0kg, natamycin 14.5kg, sodium lignin sulfonate 6.5kg, diatomite 20.0kg, in vain
Carbon black surplus.
Active constituent content accounts for 29.5% in the wettable powder.
Embodiment 4
The medicament composition that a kind of prevention bacteriosis is fallen ill is wettable powder, prepares 100kg wettable powders, packet
Include following component：Epsilon-polylysine 30.0kg, natamycin 21.0kg, sodium lignin sulfonate 12.5kg, diatomite 12.5kg, in vain
Carbon black surplus.
Active constituent content accounts for 51% in the wettable powder.
Embodiment 5
The medicament composition that a kind of prevention bacteriosis is fallen ill is wettable powder, prepares 100kg wettable powders, packet
Include following component：Epsilon-polylysine 65.0kg, natamycin 5.0kg, lignin sulfonic acid 12.5kg, diatomite 7.5kg, white carbon
Surplus.
Active constituent content accounts for 70% in the wettable powder.
Application example 1
Epsilon-polylysine, natamycin mix preparation are detected to the preventive and therapeutic effect of bacterial blight of rice.
This test arrangement is on probationly Plain plot, soil is loam in Shenyang City, Liaoning Province Shujiatun District.Cultivation management
Specification progress is uniformly produced according to experimental plot.
1. material to be tested
Medicament：The wettable powder of embodiment 1 dilutes 600 times of uses；Natamycin, epsilon-polylysine single dose, comparison medicine
Agent is 20% Yekuzuo of antibacterium disease pesticide, and spray clear water is blank control.
Pathogen：Xanthomonas campestris rice varieties (Xanthomonas oryzae pv.oryzae)
Host plant：Rice
2. test method
Experimental plot area is 60 square metres, and there are about 650 caves, four repetitions for each cell.In rice seedling bed phase (fragmentary hair
When sick) it sprays for the first time, even spray 2 times are spaced 7-10 days, and it is 45L/ mus to spray liquid volume.
3. investigation method
Whole plant in the every cell of investigation, investigation method is in strict accordance with pesticide field efficacy medicine test criterion-bactericidal agent for preventing and treating
Rice leaf diseases (GB/T 17980.19-2000) carry out.Every plant of investigation boot leaf and the following two panels leaf of boot leaf.
Bacterial blight of rice feelings grade scale is as follows：
0 grade：It is disease-free；
1 grade：Lesion area is leaf area below 10%；
3 grades：Lesion area is leaf area 11%-25%；
5 grades：Lesion area is leaf area 26%-45%；
7 grades：Lesion area is leaf area 46%-65%；
9 grades：Lesion area is more than 65% leaf area.
Calculation formula is carried out in strict accordance with GB/T 17980.19-2000.
CK0：Disease index before blank district dispenser；
CK1：Disease index after blank district dispenser；
PT0：Disease index before chemicals treatment district dispenser；
PT1：Disease index after chemicals treatment district dispenser；
4. result of the test
Each processing variation disease index and preventive effect are as follows：
| Chemicals treatment | Before dispenser | After dispenser | Control effect |
| Embodiment 1 | 10.62 | 3.45 | 86.28 |
| Natamycin | 11.31 | 11.10 | 58.51 |
| Epsilon-polylysine | 10.44 | 9.57 | 61.23 |
| Yekuzuo | 10.37 | 6.01 | 75.49 |
| Blank control | 11.25 | 26.60 | ----- |
5. conclusion：
4.5% epsilon-polylysine and natamycin mixture can prevent rice bacterial leaf spot as anti-phytobacterial disease agent
Disease, and preventive effect is notable.
Application example 2
Detect the preventive and therapeutic effect of epsilon-polylysine and natamycin mix preparation to tomato disease bacterial wilt.
This test arrangement is on probationly Plain plot, soil is sandy loam in level in Anshan city, liaoning province.Cultivation management is according to examination
It tests field and uniformly produces specification progress.
1. material to be tested
Medicament：The wettable powder of embodiment 2 dilutes 1200 times of uses；Natamycin, epsilon-polylysine single dose, control
Medicament is 20% Thiodiazole-copper, and spray clear water is blank control.
Pathogen：Eggplant Ralstonia solanacarum (Ralstonia solanacearum)
Host plant：Tomato
2. test method
Experiment sets 5 processing altogether, and 4 repetitions, totally 20 cells, random district's groups arrange.60 plants of tomato is transplanted per cell, is adopted
Take pouring root method, every plant of filling 250mL.In tomato period first time dispenser, company applies 2 times, is spaced 7-10 days.
3. investigation method
Whole plant in the every cell of investigation, calculate diseased plant rate, convert preventive effect.
4. result of the test
Each processing diseased plant rate and preventive effect are as follows：
| Chemicals treatment | Diseased plant rate (%) | Control effect (%) |
| Embodiment 2 | 2.1 | 84.6 |
| Epsilon-polylysine | 7.1 | 61.4 |
| Natamycin | 7.8 | 57.6 |
| Thiodiazole-copper | 4.9 | 73.5 |
| Blank control | 18.4 | ----- |
5. conclusion
9.5% epsilon-polylysine and natamycin mixture have notable preventive effect to bacterial wilt of tomato, be superior to polylysine,
The preventive effect that natamycin is used alone, while better than 20% Thiodiazole-copper of comparison medicament.
Application example 3
Detect the inhibiting effect of epsilon-polylysine and natamycin mix preparation to soft rot of Chinese cabbage.
1. material to be tested
Medicament：The wettable powder of embodiment 3 dilutes 3000 times of uses；Natamycin, epsilon-polylysine single dose, control
Medicament is 72% agricultural streptomycin, and spray clear water is blank control.
Pathogen：Carrot soft rot Pectinatus carrot subspecies (Pectobacterium carotovorum
subsp.carotovorum)
Host plant：Chinese cabbage
2. test method
Test site：Shenyang City Xinmin City great Ming Tun towns.
Experiment sets 5 processing altogether, 4 repetitions, totally 20 cells.The random district's groups arrangement of cell, plot area 60m2,
Strain, line-spacing are respectively 18cm, 50cm, and the total strain number of every 1 cell plantation is 210 plants.
Application method：Above each chemicals treatment respectively 10 after Chinese cabbage is transplanted, 16,27d pouring roots prevent 1 time, every plant of filling
Liquid 200mL, altogether dispenser 3 times.
3. investigation method：
The diseased plant number of each processing is investigated, and counts incidence, and the prevention effect for calculating each processing is compared with clear water
Fruit.
4. result of the test：
Each processing issues disease rate and control effect is as follows：
| Chemicals treatment | Incidence (%) | Control effect (%) |
| Embodiment 3 | 5.32 | 82.5 |
| Epsilon-polylysine | 10.82 | 64.4 |
| Natamycin | 12.98 | 57.3 |
| Agricultural streptomycin | 8.66 | 71.5 |
| Blank control | 30.4 | ----- |
5. conclusion
29.5% epsilon-polylysine and natamycin mix preparation significantly inhibit soft rot of Chinese cabbage, show
It writes better than its single dose and comparison medicament.
Application example 4
Detect the inhibiting effect of epsilon-polylysine and natamycin mix preparation to angular leaf spot of cucumber.
1. material to be tested
Medicament：The wettable powder of embodiment 4 dilutes 5000 times of uses；Natamycin, epsilon-polylysine single dose, control
Medicament is 72% agricultural streptomycin, and spray clear water is blank control.
Pathogen：Pseudomonas syringae cucumber bacterium angular leaf spot pvs oryzae and oryzicola (Pseudomonas syringae
pv.lachrymans)
Host plant：Cucumber
2. test method
Test site：The sub- town in Chaoyang City Lingyuan City small city.
Experiment sets 5 processing altogether, each handles 4 repetitions, and totally 20 cell experimental plots are arranged using random district's groups,
Each plot area 25m2。
Application method：Above each chemicals treatment 10 days before angular leaf spot of cucumber early stage (or Production of Large Fields find it is slight
During symptom) start to spray, altogether dispenser 3 times primary every 4-5 days, it is 50L/ mus to spray liquid volume.
3. investigation method：
Take 5 points of investigation at random per cell, every whole blades of 2 plants of investigation account for the hundred of entire leaf area by scab on blade
Rate classification is divided to calculate sick leaf rate, disease index and control effect.
Grade scale is：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than 5% entire leaf area；
3 grades：Lesion area accounts for the 6%-10% of entire leaf area；
5 grades：Lesion area accounts for the 11%-20% of entire leaf area；
7 grades：Lesion area accounts for the 21%-50% of entire leaf area；
9 grades：Lesion area accounts for more than 50% entire leaf area；
CK0：Disease index before blank district dispenser；
CK1：Disease index after blank district dispenser；
PT0：Disease index before chemicals treatment district dispenser；
PT1：Disease index after chemicals treatment district dispenser；
4. result of the test：
Each processing issues disease disease index and control effect is as follows：
| Chemicals treatment | The state of an illness before dispenser | The state of an illness after dispenser | Control effect |
| Embodiment 4 | 17.18 | 3.52 | 85.84 |
| Natamycin | 18.11 | 12.55 | 52.16 |
| ε-poly- bad | 15.09 | 7.75 | 64.54 |
| Agricultural strepto- | 17.41 | 5.98 | 76.29 |
| Blank control | 18.78 | 27.20 | ----- |
5. conclusion
51% epsilon-polylysine and natamycin mix preparation significantly inhibit cucumber bacterial angular leaf spot,
It is significantly better than its single dose and comparison medicament.
Application example 5
Detect the inhibiting effect of epsilon-polylysine and natamycin mix preparation to wildfire.
1. material to be tested
Medicament：The wettable powder of embodiment 5 dilutes 8000 times of uses；Natamycin, epsilon-polylysine single dose, control
Medicament is 20% Thiodiazole-copper, and spray clear water is blank control.
Pathogen：Tobacco Pseudomonas alba (Pseudomonas syringae pv.tabaci)
Host plant：Tobacco
2. test method
Test site：Tieling Xifeng County He Long townshiies.
Experiment sets 5 processing altogether, 3 repetitions, totally 24 cells.The random district's groups arrangement of cell, plot area 66m2, strain
Line-spacing is 0.55m × 1.1m, and the total strain number of every 1 cell plantation is 100 plants.
Application method：Above each chemicals treatment 10 days before wildfire early stage (or Production of Large Fields find it is slight
During symptom) start to spray, altogether dispenser 2 times primary every 10 days, it is 50L/ mus to spray liquid volume.
3. investigation method：
Every plant of cigarette investigates 10 leaves from the bottom to top, and is compared with clear water and calculate each processing disease index and prevention effect
Fruit.
Wildfire disease severity grade scale (as unit of blade)：
0 grade：Full leaf is disease-free；
0.5 grade：Lesion area accounts for less than 1% blade area；
1 grade：Lesion area accounts for the 1%-5% of blade area；
2 grades：Lesion area accounts for the 5%-10% of blade area；
3 grades：Lesion area accounts for the 10%-20% of blade area；
4 grades：Lesion area accounts for more than 20% blade area；
Disease index (%)=[∑ (disease grade × this grade of number of sheets)/(the investigation number of sheets × most serious grade)] × 100
CK0：Disease index before blank district dispenser；
CK1：Disease index after blank district dispenser；
PT0：Disease index before chemicals treatment district dispenser；
PT1：Disease index after chemicals treatment district dispenser；
4. result of the test：
Each processing issues disease disease index and control effect is as follows：
| Chemicals treatment | The state of an illness before dispenser | The state of an illness after dispenser | Control effect |
| Embodiment 5 | 8.31 | 2.57 | 87.13 |
| Natamycin | 7.15 | 6.64 | 61.16 |
| ε-poly- bad | 8.82 | 7.74 | 63.28 |
| Thiodiazole-copper | 12.24 | 7.12 | 75.65 |
| Blank control | 9.33 | 22.30 | ----- |
5. conclusion
70% epsilon-polylysine and natamycin mix preparation significantly inhibit wildfire, significantly excellent
In its single dose and comparison medicament.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of medicament composition of the prevention phytobacterial disease containing epsilon-polylysine, which is characterized in that including following component：
Epsilon-polylysine, natamycin and filler.
2. the medicament composition of the prevention phytobacterial disease containing epsilon-polylysine as described in claim 1, which is characterized in that
The filler and auxiliary agent includes：Diatomite, white carbon and sodium lignin sulfonate.
3. the medicament composition of the prevention phytobacterial disease containing epsilon-polylysine as described in claim 1, which is characterized in that
The medicament composition can be processed into 1%~70% wettable powder.
4. the medicament composition of the prevention phytobacterial disease containing epsilon-polylysine as described in claim 1, which is characterized in that
It can be any one in vegetables, fruit tree, crops, flower plant bacteriosis that the bacteriosis, which is fallen ill,.
5. the medicament composition of the prevention phytobacterial disease containing epsilon-polylysine as claimed in claim 4, which is characterized in that
The phytobacterial disease can be bacterial blight of rice, bacterial wilt of tomato, soft rot of Chinese cabbage, angular leaf spot of cucumber, tobacco
Any one in wildfire.
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| CN201810084338.8A CN108184873A (en) | 2018-01-29 | 2018-01-29 | A kind of medicament composition of the prevention phytobacterial disease containing epsilon-polylysine |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108947688A (en) * | 2018-08-24 | 2018-12-07 | 桐城市天泰农业种植专业合作社 | A kind of wheat planting special bio charcoal compound organic fertilizer |
| CN113767909A (en) * | 2021-09-17 | 2021-12-10 | 海南大学 | Use of epsilon-polylysine |
| CN113767910A (en) * | 2021-09-17 | 2021-12-10 | 海南大学 | Use of epsilon-polylysine |
| CN113785842A (en) * | 2021-10-20 | 2021-12-14 | 海南大学 | Pesticide composition for preventing and treating plant fungal diseases and preparation method thereof |
| CN113875779A (en) * | 2021-10-20 | 2022-01-04 | 海南大学 | Bactericide composition and preparation method thereof |
| CN114938809A (en) * | 2022-04-29 | 2022-08-26 | 中国农业科学院植物保护研究所 | Compound composition of wuyiencin and epsilon-polylysine, sterilization method and application |
| CN115152763A (en) * | 2022-07-06 | 2022-10-11 | 西南大学 | Rod-like nanocrystallization method of epsilon-polylysine and application of epsilon-polylysine nanoparticles |
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| CN102389743A (en) * | 2011-06-14 | 2012-03-28 | 陈旭良 | Formula of medical bacteriostatic humidifying solution |
| CN106719783A (en) * | 2016-11-22 | 2017-05-31 | 张有林 | A kind of jujube blackhead disease pesticide control |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389743A (en) * | 2011-06-14 | 2012-03-28 | 陈旭良 | Formula of medical bacteriostatic humidifying solution |
| CN106719783A (en) * | 2016-11-22 | 2017-05-31 | 张有林 | A kind of jujube blackhead disease pesticide control |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108947688A (en) * | 2018-08-24 | 2018-12-07 | 桐城市天泰农业种植专业合作社 | A kind of wheat planting special bio charcoal compound organic fertilizer |
| CN113767909A (en) * | 2021-09-17 | 2021-12-10 | 海南大学 | Use of epsilon-polylysine |
| CN113767910A (en) * | 2021-09-17 | 2021-12-10 | 海南大学 | Use of epsilon-polylysine |
| CN113785842A (en) * | 2021-10-20 | 2021-12-14 | 海南大学 | Pesticide composition for preventing and treating plant fungal diseases and preparation method thereof |
| CN113875779A (en) * | 2021-10-20 | 2022-01-04 | 海南大学 | Bactericide composition and preparation method thereof |
| CN113875779B (en) * | 2021-10-20 | 2022-10-28 | 海南大学 | Bactericide composition and preparation method thereof |
| CN114938809A (en) * | 2022-04-29 | 2022-08-26 | 中国农业科学院植物保护研究所 | Compound composition of wuyiencin and epsilon-polylysine, sterilization method and application |
| CN115152763A (en) * | 2022-07-06 | 2022-10-11 | 西南大学 | Rod-like nanocrystallization method of epsilon-polylysine and application of epsilon-polylysine nanoparticles |
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