CN108165490A - Cell culture of animals device - Google Patents
Cell culture of animals device Download PDFInfo
- Publication number
- CN108165490A CN108165490A CN201810019590.0A CN201810019590A CN108165490A CN 108165490 A CN108165490 A CN 108165490A CN 201810019590 A CN201810019590 A CN 201810019590A CN 108165490 A CN108165490 A CN 108165490A
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- China
- Prior art keywords
- culture dish
- annulus
- pieces
- culture
- cell culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides cell culture of animals device, including culture dish device and rectangular thread tooth；The culture dish device is collectively constituted by culture dish body, arc panel, groove, secter pat, rectangular thread slot, threaded hole and fanning strip；One piece of culture dish lid is provided with above the sealing mechanism；One piece of clamp system is provided with above the culture dish lid；The clamp system is collectively constituted by clamping annulus, push rod and rectangular thread tooth；The top end face for clamping annulus is fixedly connected with a push rod.The sealing mechanism that the present invention passes through setting, it can be before culture dish lid fasten with culture dish body, the openend held in culture dish body can tightly be sealed by the silica gel thin-layer in sealing mechanism, pass through the sealed set of sealing mechanism of the present invention, if detached accidentally with culture dish body conducive to culture dish lid, the cell which can be guaranteed in culture dish body will not leak.
Description
Technical field
The invention belongs to cell culture apparatus technical field more particularly to cell culture of animals devices.
Background technology
At present, application of the culture dish in cell or field of microbial culture technology has many decades, often in the lab
It uses.Culture dish is typically circular, is made of the ware body of receiving culture medium and the ware lid of matching.Ware lid is positioned on ware body,
Culture medium can be prevented by pollutions such as the microorganisms in external environment, while air is allowed to pass through depositing in favor of cell or microorganism
Living, growth and observation, detection.
Based on above-mentioned, inventor has found, when smooth culture dish in operator's long-time operation surface is also easy to produce the feelings such as landing
Condition is also easy to produce and accidentally grabs ware lid, gently then pollutes the culture in culture dish, heavy then culture dish may be caused to damage；In order to
Ensure gas permeability, ware and lid between there are gap it is larger, during taking, can only often pinch lid, thus ware and cover very
It can be easily separated and easily tilt and spill leakage, these situations can all influence the process and result of Bioexperiment.
Such as application number：The present invention relates to a kind of sampling microorganisms from surface of object culture dishes by CN201210190400.4, should
Culture dish includes round platform pedestal 1, assembly outer wall 2, culture dish cover 3, and assembly outer wall protrusion 4 and is lived at round platform pedestal 1 therewith
The dynamic assembly outer wall 2 connected forms culture dish ware body, using the frictional force of material in itself, and using interference fit and geometry certainly
Lock realizes being fastenedly connected for ware body, and assembly outer wall protrusion 4 is used for supporting culture dish cover 3, prevents the upper bottom of culture dish cover 3
Culture medium is touched, said combination connection mode ensure that the seal and aseptic of culture dish.The configuration of the present invention is simple makes
With convenient and efficient, the step of simplifying sampling microorganisms from surface of object culture, and ensure that the seal and aseptic of culture dish,
The not easy infection miscellaneous bacteria in sampling process, improve body surface microorganism detection sampling efficiency and sample result it is accurate
Rate.
Such as application number：CN201320617968.X is the present invention relates to experimental ware field, in particular to culture
Ware, including culture dish ontology and culture dish lid, culture dish ontology is the hollow cylinder of one end open, is provided on openend
Notch, notch are protruded towards the axis direction far from culture dish ontology, and the liquid in culture dish ontology is poured from notch；Culture
Ware lid is used to cover in the openend of culture dish ontology and notch.Culture dish provided by the invention, by culture dish ontology
On be provided with notch, and notch is protruded towards the axis direction far from culture dish ontology.When pipetting solution from culture dish ontology,
Solution pours out culture dish ontology from notch, thus avoid solution trickling it is external to culture dish sheet, reduce cell or tissue and exist
Contaminated probability in incubation.
Culture dish designed by above-mentioned two patent with existing as the culture dish used, exist when culture dish lid with
When culture dish body detaches accidentally, easily cause cell leak and it is excessive want to contact with outside air cause cell contaminated
Problem.
Then, in view of this, improvement is studied for existing structure and missing, cell culture of animals dress is provided
It puts, to achieve the purpose that with more more practical value.
Invention content
In order to solve the above technical problem, the present invention provides cell culture of animals devices, long to solve existing operator
Situations such as being also easy to produce landing during the smooth culture dish in time operation surface is also easy to produce and accidentally grabs ware lid, gently then pollutes culture
Culture in ware is heavy then culture dish may be caused to damage；In order to ensure gas permeability, between ware and lid there are gap it is larger,
During taking, lid can only be often pinched, so ware and lid are very easy to separation and easily tilt and spill leakage, these situations all can
The problem of influencing the process and result of Bioexperiment.
The purpose and effect of cell culture of animals device of the present invention, are reached by technological means in detail below：
Cell culture of animals device, including culture dish device, culture dish body, arc panel, groove, secter pat, rectangle spiral shell
Groove, threaded hole, fanning strip, culture dish lid, sealing mechanism, annulus, silica gel thin-layer, sector connection block, arc block, counter sink,
Bolt, clamps annulus, push rod and rectangular thread tooth at clamp system；The culture dish device by culture dish body, arc panel, groove,
Secter pat, rectangular thread slot, threaded hole and fanning strip collectively constitute；The culture dish body bottom face circular array shape, which is fixed, to be connected
Three pieces of fanning strips are connected to, and one piece of arc has been fixedly connected at the head end position of three pieces of fanning strip top end faces
Plate；The first half of the inner face of three pieces of arc panels offers the rectangular thread slot of corresponding cooperation jointly；Described three
The center of arc panel top end face described in block offers the groove at one, and three pieces of arc panel inner face lower half
Center position is both provided with one piece of secter pat；Three pieces of secter pat top end face centers offer described at one
Threaded hole；Sealing mechanism described in one group is provided with above the culture dish device；The sealing mechanism is thin by annulus, silica gel
Layer, sector connection block, arc block and counter sink collectively constitute；The outer wall circular array shape of the annulus is by described in one piece
Sector connection block is fixedly connected with one piece of arc block；Three pieces of sector connection block top end face centers offer one
Locate the counter sink；The annulus inner end is inlaid with one layer of silica gel thin-layer；Three bolt tail ends are each passed through three pieces
The counter sink that the sector connection block top end face is opened up simultaneously is offered with three pieces of secter pat top end faces respectively
The threaded hole screw thread is connected；One piece of culture dish lid is provided with above the sealing mechanism；The culture dish lid
Top is provided with one piece of clamp system；The clamp system is collectively constituted by clamping annulus, push rod and rectangular thread tooth；Institute
The top end face for stating clamping annulus is fixedly connected with a push rod.
Further, the diameter of the silica gel thin-layer inlayed in the annulus is more than the size of internal radius.
Further, the thickness of the silica gel thin-layer is 0.5cm.
Further, the internal diameter of the annulus is more than the inner end diameter of culture dish body.
Further, the diameter contour line phase of the composition between the outer diameter of the annulus and three pieces of arc panel inner faces
Unanimously.
Further, the internal diameter for clamping annulus is consistent with the inner end diameter of culture dish body.
Further, the diameter profile of the composition between the outer diameter for clamping annulus and three pieces of arc panel inner faces
Line is consistent.
Further, it is described clamp annulus outer diameter face on be provided with described in the rectangular thread slot phase thread fitting
Rectangular thread tooth.
Further, it is subtle ventilative to offer several for the center position circular array shape of the silica gel thin-layer
Hole.
Compared with prior art, the present invention has the advantages that：
The present invention can pass through sealing mechanism by the sealing mechanism of setting before culture dish lid does not fasten with culture dish body
In silica gel thin-layer the openend held in culture dish body can tightly be sealed, and by the fixation of three bolts, to ensure three
When under not being disassembled, the silica gel thin-layer in the sealing mechanism will not be separated bolt with the openend held in culture dish body, lead to
The sealed set of sealing mechanism of the present invention is crossed, if detached accidentally with culture dish body conducive to culture dish lid, which can
The cell being guaranteed in culture dish body will not leak, and the center position circular array shape of silica gel thin-layer offer it is several
A subtle air hole, ensure air draught in order to cell survival on the basis of, to greatest extent ensure culture dish lid with
Cell will not be leaked from edge under culture dish body discrete state.
The setting of clamp system of the present invention, conducive to the openend that will be held in culture dish body when the silica gel thin-layer in sealing mechanism
When tightly sealing, then by culture dish cover buckle close after, by twisting clamp system downwards so that the patch of clamp system low side face tightly
Clothes are covered in culture dish so that under the premise of non-loosening clamp system, ensure that showing for accidental separation will not occur for culture dish lid
As when air is needed to flow, it is only necessary to loosen clamp system so that culture dish lid reserves one with the silica gel thin-layer in sealing mechanism
Point gap, can ensure the circulation of air.
Description of the drawings
Fig. 1 is the disassembled form structure diagram of the present invention.
The culture dish assembly axis that Fig. 2 is the present invention regards structure diagram.
Fig. 3 is the culture dish device overlooking the structure diagram of the present invention.
The sealing mechanism axis that Fig. 4 is the present invention regards structure diagram.
Fig. 5 is the sealing mechanism overlooking the structure diagram of the present invention.
The clamp system axis that Fig. 6 is the present invention regards structure diagram.
Fig. 7 is the clamp system main structure diagram of the present invention.
The assembled state axis that Fig. 8 is the present invention regards structure diagram.
Fig. 9 is the assembled state overlooking the structure diagram of the present invention.
Figure 10 is A-A schematic cross-sectional views in Fig. 9 of the invention.
In figure：1- culture dish devices, 101- culture dish bodies, 102- arc panels, 103- grooves, 104- secter pats, 105- squares
Shape thread groove, 106- threaded holes, 107- fanning strips, 2- culture dish lids, 3- sealing mechanisms, 301- annulus, 302- silica gel thin-layers,
303- sector connection blocks, 304- arc blocks, 305- counter sinks, 4- bolts, 5- clamp systems, 501- clamping annulus, 502- push rods,
503- rectangular thread teeth.
Specific embodiment
The present invention is described further below in conjunction with attached drawing：
Embodiment：
As shown in attached drawing 1 to attached drawing 10：
The present invention provides cell culture of animals device, including culture dish device 1, culture dish body 101, arc panel 102, recessed
Slot 103, secter pat 104, rectangular thread slot 105, threaded hole 106, fanning strip 107, culture dish lid 2, sealing mechanism 3, annulus
301st, silica gel thin-layer 302, sector connection block 303, arc block 304, counter sink 305, bolt 4, clamp system 5, clamping annulus
501st, push rod 502 and rectangular thread tooth 503；The culture dish device 1 is by culture dish body 101, arc panel 102, groove 103, fan
Shape block 104, rectangular thread slot 105, threaded hole 106 and fanning strip 107 collectively constitute；101 bottom face of culture dish body is in ring
Shape array-like is fixedly connected with three pieces of fanning strips 107, and solid at the head end position of three pieces of 107 top end faces of fanning strip
Surely it is connected with one piece of arc panel 102；The first half of the inner face of three pieces of arc panels 102 offers corresponding match jointly
The rectangular thread slot 105 closed；The center of three pieces of 102 top end faces of arc panel offers described recessed at one
Slot 103, and three pieces of 102 inner face lower half center positions of arc panel are both provided with one piece of secter pat 104；Three pieces
The 104 top end face center of secter pat offers the threaded hole 106 at one；The top of the culture dish device 1 is set
It is equipped with sealing mechanism 3 described in one group；The sealing mechanism 3 is by annulus 301, silica gel thin-layer 302, sector connection block 303, arc block
304 and counter sink 305 collectively constitute；The outer wall circular array shape of the annulus 301 passes through one piece of sector connection block
303 are fixedly connected with one piece of arc block 304；Three pieces of 303 top end face centers of sector connection block offer one
Locate the counter sink 305；301 inner end of annulus is inlaid with one layer of silica gel thin-layer 302；Three 4 tail ends of bolt point
Not Chuan Guo the counter sink 305 that is opened up of three pieces of 303 top end faces of sector connection block and respectively with three pieces of secter pats
106 screw thread of the threaded hole that 104 top end faces are offered is connected；The top of the sealing mechanism 3 is provided with described in one piece
Culture dish lid 2；The top of the culture dish lid 2 is provided with one piece of clamp system 5；The clamp system 5 is by clamping annulus
501st, push rod 502 and rectangular thread tooth 503 collectively constitute；The top end face for clamping annulus 501 is fixedly connected with described in one
Push rod 502.
Wherein, the diameter of the silica gel thin-layer 302 inlayed in the annulus 301 is more than the size of 301 internal diameter of annulus,
In order to the openend of the fully wrapped around firmly culture dish body 101 of silica gel thin-layer 302 under assembled state.
Wherein, the thickness of the silica gel thin-layer 302 is 0.5cm, after wrapping the openend of culture dish body 101, behaviour
Make personnel and also can pass through the inner face that silica gel thin-layer 302 watches culture dish body 101.
Wherein, the internal diameter of the annulus 301 is more than the inner end diameter of culture dish body 101, in order to silica gel under assembled state
The openend of the fully wrapped around firmly culture dish body 101 of thin layer 302.
Wherein, the diameter contour line of the composition between the outer diameter of the annulus 301 and three pieces of 102 inner faces of arc panel
It is consistent, ensure the gripping orientation of assembled state.
Wherein, the internal diameter for clamping annulus 501 is consistent with the inner end diameter of culture dish body 101, operating personnel's viewing
To the complete situation of the inner face of culture dish body 101.
Wherein, the diameter wheel of the composition between the outer diameter for clamping annulus 501 and three pieces of 102 inner faces of arc panel
Profile is consistent, ensures the gripping orientation of assembled state.
Wherein, the institute with the 105 phase thread fitting of rectangular thread slot is provided in the outer diameter face for clamping annulus 501
Rectangular thread tooth 503 is stated, operation is clamped to culture dish lid 2 convenient for clamp system 5.
Wherein, the center position circular array shape of the silica gel thin-layer 302 offers several subtle air holes,
Air to be allowed to pass through the survival in favor of cell or microorganism.
The specifically used mode of the present embodiment and effect：
It, can be by three groups of sector connection blocks 303 of annulus 301 in sealing mechanism 3 after cell is added into culture dish body 101
At be respectively embedded in culture dish device 1 that arc panel 102 is opened up with arc block 304 three in groove 103, and by three spiral shells
Bolt 4 passes through three pieces of sector connection block top end faces countersunk head opened up and the screw thread offered respectively with three pieces of secter pat top end faces
Hole screw thread is connected, and when constantly tightening three bolts 4 downwards, sealing mechanism 3 constantly pushes so that in sealing mechanism 3
Silica gel thin-layer 302 be also constantly stretched so that its openend with culture dish body 101 is mutually close to, when sealing mechanism 3 with training
It supports after ware device 1 fixes, then culture dish lid 2 is fastened on the silica gel thin-layer 302 at 101 position of culture dish body, then will
Clamp system 5 is made by the cooperation of rectangular thread slot 105 that rectangular thread tooth 503 is opened up with three pieces of 104 inner faces of secter pat
It obtains and twists clamp system 5 downwards, it is made to cause the docile of 5 low side face of clamp system tightly on culture dish lid 2 so that in no pine
Under the premise of dynamic clamp system 5, ensure the phenomenon that accidental separation will not occur for culture dish lid 2, when air is needed to flow, it is only necessary to
Loosen clamp system 5 so that culture dish lid 2 reserves some gaps with the silica gel thin-layer 302 in sealing mechanism 3, can ensure sky
The circulation of gas.
Using technical solution of the present invention or those skilled in the art under the inspiration of technical solution of the present invention, if
Similar technical solution is counted out, and reaches above-mentioned technique effect, is to fall into protection scope of the present invention.
Claims (9)
1. cell culture of animals device, it is characterised in that：The cell culture of animals device includes culture dish device, culture dish
Body, arc panel, groove, secter pat, rectangular thread slot, threaded hole, fanning strip, culture dish lid, sealing mechanism, annulus, silica gel are thin
Layer, arc block, counter sink, bolt, clamp system, clamps annulus, push rod and rectangular thread tooth at sector connection block；The culture
Ware device is collectively constituted by culture dish body, arc panel, groove, secter pat, rectangular thread slot, threaded hole and fanning strip；The training
It supports ware body bottom face circular array shape and is fixedly connected with three pieces of fanning strips, and the head end of three pieces of fanning strip top end faces
One piece of arc panel has been fixedly connected at position；The first half of the inner face of three pieces of arc panels offers relatively jointly
The rectangular thread slot that should coordinate；The center of three pieces of arc panel top end faces offers described recessed at one
Slot, and three pieces of arc panel inner face lower half center positions are both provided with one piece of secter pat；Three pieces of sectors
Block top end face center offers the threaded hole at one；Sealing described in one group is provided with above the culture dish device
Mechanism；The sealing mechanism is collectively constituted by annulus, silica gel thin-layer, sector connection block, arc block and counter sink；The annulus
Outer wall circular array shape is fixedly connected with one piece of arc block by one piece of sector connection block；Three pieces of sectors
Link block top end face center offers the counter sink at one；It is thin that the annulus inner end is inlaid with one layer of silica gel
Layer；Three bolt tail ends be each passed through the counter sink that three pieces of sector connection block top end faces are opened up and respectively with
The threaded hole screw thread that three pieces of secter pat top end faces are offered is connected；One is provided with above the sealing mechanism
Culture dish lid described in block；One piece of clamp system is provided with above the culture dish lid；The clamp system is justified by clamping
Ring, push rod and rectangular thread tooth collectively constitute；The top end face for clamping annulus is fixedly connected with a push rod.
2. cell culture of animals device as described in claim 1, it is characterised in that：The silica gel inlayed in the annulus
The diameter of thin layer is more than the size of internal radius.
3. cell culture of animals device as claimed in claim 1 or 2, it is characterised in that：The thickness of the silica gel thin-layer is
0.5cm。
4. cell culture of animals device as claimed in claim 1 or 2, it is characterised in that：The internal diameter of the annulus is more than culture
The inner end diameter of ware body.
5. the cell culture of animals device as described in claims 1 or 2 or 4, it is characterised in that：The outer diameter of the annulus and three pieces
The diameter contour line of composition between the arc panel inner face is consistent.
6. the cell culture of animals device as described in claim 1 or 4, it is characterised in that：The internal diameter for clamping annulus and training
The inner end diameter for supporting ware body is consistent.
7. the cell culture of animals device as described in claim 1 or 6, it is characterised in that：The outer diameter and three for clamping annulus
The diameter contour line of composition between arc panel inner face described in block is consistent.
8. the cell culture of animals device as described in claim 1 or 6 or 7, it is characterised in that：The outer diameter face for clamping annulus
On be provided with the rectangular thread tooth with the rectangular thread slot phase thread fitting.
9. the cell culture of animals device as described in claims 1 or 2 or 3, it is characterised in that：The center of the silica gel thin-layer
Position circular array shape offers several subtle air holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810019590.0A CN108165490A (en) | 2018-01-09 | 2018-01-09 | Cell culture of animals device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810019590.0A CN108165490A (en) | 2018-01-09 | 2018-01-09 | Cell culture of animals device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108165490A true CN108165490A (en) | 2018-06-15 |
Family
ID=62517998
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810019590.0A Withdrawn CN108165490A (en) | 2018-01-09 | 2018-01-09 | Cell culture of animals device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108165490A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122425A (en) * | 2021-03-08 | 2021-07-16 | 孔祥玲 | Stem cell centrifugal equipment capable of being fixed quickly and fixing method |
-
2018
- 2018-01-09 CN CN201810019590.0A patent/CN108165490A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122425A (en) * | 2021-03-08 | 2021-07-16 | 孔祥玲 | Stem cell centrifugal equipment capable of being fixed quickly and fixing method |
| CN113122425B (en) * | 2021-03-08 | 2022-07-26 | 上海揽微赛尔生物科技有限公司 | Stem cell centrifugal equipment capable of being fixed quickly and fixing method |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20180615 |