CN108143956A - 预防猪副伤寒病的制剂及其制备方法 - Google Patents
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Abstract
本发明公开了预防猪副伤寒病的制剂的制备方法，通过向水中加入20‑24份麦门冬、9‑12份黄芩提取物、21‑25份稗米、15‑20份白头翁、8‑12份大青叶、4‑7份桂枝，经煎煮制得预防猪副伤寒病的制剂。有益效果为：本发明所公布的预防猪副伤寒病的制剂的制备方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效；黄芩提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效。
Description
技术领域
本发明涉及兽药领域，尤其是涉及预防猪副伤寒病的制剂及其制备方法。
背景技术
猪副伤寒是猪的一种常见病，病原为沙门氏杆菌，主要表现为猪发高烧，体温在40℃以上，精神不振，腹痛、下痢，粪便呈淡黄或灰绿色，并且恶臭，严重可发生坏死性肠炎，死亡率较高。由于近来本病普遍发生，具有传染性，加上用药普遍，造成病原耐药性严重，并且本病临床表现的高烧与腹泻同时进行，容易导致抵抗力下降，继发其他细菌感染，并且很容易造成体质差的猪产生衰竭，采取一般常规手段、药物的单纯治疗，往往疗效不佳，造成的经济损失较大。
发明内容
本发明的目的是提供预防猪副伤寒病的制剂的制备方法，该方法工艺简单，材料易得，费用低廉，所制备的制剂能够减少病原耐药性，预防继发感染。
为了达到上述目的，本发明提供的技术方案为：预防猪副伤寒病的制剂的制备方法，过程为：向煎煮器皿中加入20-24份麦门冬、9-12份黄芩提取物、21-25份稗米、15-20份白头翁、8-12份大青叶、4-7份桂枝以及120-160份水，煎煮，去渣取汁，得预防猪副伤寒病的制剂。工艺方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效。
作为优选，预防猪副伤寒病的制剂的制备中煎煮过程为：首先在190-210℃煎煮20-35min，接着在125-135℃煎煮9-13min。该条件下，采用两段蒸煮过程，降低了高温对原料活性成分结构的破坏，同时缩短了煎煮时间。
作为优选，黄芩提取物的制备方法包括以下步骤：首先是提取：向黄芩根粉末中加入乙醇溶液，调pH为4-5，超声提取，离心分离取上清液；接着是吸附：向上清液中加入Fe3O4磁性纳米粒子，在15-25℃恒温振荡仪上振荡15-20min，磁铁分离，使溶液变为上下两相；最后为解吸附：去除上清液，将Fe3O4磁性纳米粒子吸附的黄芩提取物洗脱，洗脱液经减压蒸发得黄芩提取物。该提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效。
作为优选，黄芩提取物的制备中乙醇溶液的浓度为90％-95％，黄芩根粉末与乙醇溶液的料液比为1g:3-6mL。该条件下，黄芩活性成分的提取率高，且该溶剂可以回收重复使用，大大降低了生产成本。
作为优选，黄芩提取物的制备中超声频率为35-45kHz，超声时间为20-30min。该条件下，使混合物能够形成均匀的混合物，提高了制剂剂的品质，且该频率下所生成的混合物性能稳定，不易分层。
作为优选，黄芩提取物的制备中洗脱所用溶剂为体积比2-6:100的乙胺与乙醇组成的混合溶剂。该条件下，所用溶剂的洗脱效率高，大大提高了黄芩活性成分的提取率，使黄芩资源得到了充分利用。
与现有技术相比，本发明的有益效果为：本发明所公布的预防猪副伤寒病的制剂的制备方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效；黄芩提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效。
具体实施方式
以下通过实施例进一步详细描述：
实施例1：预防猪副伤寒病的制剂的制备方法，包括以下步骤：
1)黄芩提取物的制备：首先按料液比1g:3mL向黄芩根粉末中加入浓度为90％的乙醇溶液，调pH为4，在35kHz频率下超声提取20min，在1000rpm下离心分离4min，取上清液，接着向上清液中加入占上清液质量2％的Fe3O4磁性纳米粒子，在15℃恒温振荡仪上振荡15min，磁铁分离，使溶液变为上下两相，最后去除上清液，采用体积比2:100的乙胺与乙醇组成的混合溶剂将Fe3O4磁性纳米粒子吸附的黄芩提取物洗脱，洗脱液经减压蒸发得黄芩提取物，该提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效；
2)预防猪副伤寒病的制剂的制备：向煎煮器皿中加入20份麦门冬、9份黄芩提取物、21份稗米、15份白头翁、8份大青叶、4份桂枝以及120份水，在190℃煎煮20min，接着在125℃煎煮9min，去渣取汁，得预防猪副伤寒病的制剂，工艺方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效。
实施例2：预防猪副伤寒病的制剂的制备方法，包括以下步骤：
1)黄芩提取物的制备：首先按料液比1g:6mL向黄芩根粉末中加入浓度为95％的乙醇溶液，调pH为5，接着加入占乙醇质量0.01％的3,5-二羟基苯乙酮和占乙醇质量0.012％的戊酸乙酯，在45kHz频率下超声提取30min，在1000rpm下离心分离4min，取上清液，接着向上清液中加入占上清液质量2％的Fe3O4磁性纳米粒子，在25℃恒温振荡仪上振荡20min，磁铁分离，使溶液变为上下两相，最后去除上清液，采用体积比6:100的乙胺与乙醇组成的混合溶剂将Fe3O4磁性纳米粒子吸附的黄芩提取物洗脱，洗脱液经减压蒸发得黄芩提取物，该提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效，所加入的3,5-二羟基苯乙酮、戊酸乙酯与水杨酸钠具有协同作用，显著提高了黄芩的活性成分在乙醇溶剂中生成可溶性络合物的速率，提高了黄芩的活性成分的提取效率，使黄芩资源得到了充分利用，降低了最终的成本；
2)预防猪副伤寒病的制剂的制备：向煎煮器皿中加入24份麦门冬、12份黄芩提取物、25份稗米、20份白头翁、12份大青叶、7份桂枝以及160份水，在210℃煎煮35min，接着在135℃煎煮13min，去渣取汁，得预防猪副伤寒病的制剂，工艺方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效。
实施例3：预防猪副伤寒病的制剂的制备方法，包括以下步骤：
1)黄芩提取物的制备：首先按料液比1g:4mL向黄芩根粉末中加入浓度为92％的乙醇溶液，调pH为4，在40kHz频率下超声提取25min，在1000rpm下离心分离4min，取上清液，接着向上清液中加入占上清液质量2％的Fe3O4磁性纳米粒子，在17℃恒温振荡仪上振荡18min，磁铁分离，使溶液变为上下两相，最后去除上清液，采用体积比4:100的乙胺与乙醇组成的混合溶剂将Fe3O4磁性纳米粒子吸附的黄芩提取物洗脱，洗脱液经减压蒸发得黄芩提取物，该提取条件温和，对黄芩活性成分的结构破坏小，且提取的多种活性成分具有协同作用，提高了制剂的药效，使制剂达到了良好的疗效；
2)预防猪副伤寒病的制剂的制备：向煎煮器皿中加入22份麦门冬、10份黄芩提取物、22份稗米、17份白头翁、9份大青叶、5份桂枝以及140份水，在195℃煎煮27min，接着在128℃煎煮10min，去渣取汁，得预防猪副伤寒病的制剂，工艺方法简单，条件温和，产品质量稳定性好，材料易得，费用低廉，能够减少病原耐药性，预防继发感染，具有显著的疗效。
以上实施方式仅用于说明本发明，而并非对本发明的限制，本领域的普通技术人员，在不脱离本发明的精神和范围的情况下，还可以做出各种变化和变型。因此，所有等同的技术方案也属于本发明的范畴，本发明的专利保护范围应由权利要求限定。
Claims (7)
1.预防猪副伤寒病的制剂的制备方法，其特征在于：所述制备方法为：向煎煮器皿中加入20-24份麦门冬、9-12份黄芩提取物、21-25份稗米、15-20份白头翁、8-12份大青叶、4-7份桂枝以及120-160份水，煎煮，去渣取汁，得预防猪副伤寒病的制剂。
2.根据权利要求1所述的预防猪副伤寒病的制剂的制备方法，其特征在于：所述煎煮过程为：首先在190-210℃煎煮20-35min，接着在125-135℃煎煮9-13min。
3.根据权利要求1所述的预防猪副伤寒病的制剂的制备方法，其特征在于：所述黄芩提取物的制备方法包括以下步骤：
1)提取：向黄芩根粉末中加入乙醇溶液，调pH为4-5，超声提取，离心分离取上清液；
2)吸附：向上清液中加入Fe3O4磁性纳米粒子，在15-25℃恒温振荡仪上振荡15-20min，磁铁分离，使溶液变为上下两相；
3)解吸附：去除上清液，将Fe3O4磁性纳米粒子吸附的黄芩提取物洗脱，洗脱液经减压蒸发得黄芩提取物。
4.根据权利要求3所述的预防猪副伤寒病的制剂的制备方法，其特征在于：所述步骤1)中乙醇溶液的浓度为90％-95％，黄芩根粉末与乙醇溶液的料液比为1g:3-6mL。
5.根据权利要求3所述的预防猪副伤寒病的制剂的制备方法，其特征在于：所述步骤1)中超声频率为35-45kHz，超声时间为20-30min。
6.根据权利要求3所述的预防猪副伤寒病的制剂的制备方法，其特征在于：所述步骤3)中洗脱所用溶剂为体积比2-6:100的乙胺与乙醇组成的混合溶剂。
7.权利要求1-6任一项所述方法制备的预防猪副伤寒病的制剂。
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| KR20100128098A (ko) * | 2009-05-27 | 2010-12-07 | 전북대학교산학협력단 | 돼지 인터류킨-18 유전자를 발현하는 살모넬라 균주 및 이를 함유하는 경구 투여용 조성물 |
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| CN103768351A (zh) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | 治疗仔猪副伤寒的中药组合物及其制备方法 |
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| CN102659877A (zh) * | 2012-05-16 | 2012-09-12 | 湖南师范大学 | 一种从黄芩根中提取黄芩苷和黄芩素的方法 |
| CN103768351A (zh) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | 治疗仔猪副伤寒的中药组合物及其制备方法 |
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