CN108142359A - A kind of method for improving sow reproductive performance - Google Patents
A kind of method for improving sow reproductive performance Download PDFInfo
- Publication number
- CN108142359A CN108142359A CN201711473846.7A CN201711473846A CN108142359A CN 108142359 A CN108142359 A CN 108142359A CN 201711473846 A CN201711473846 A CN 201711473846A CN 108142359 A CN108142359 A CN 108142359A
- Authority
- CN
- China
- Prior art keywords
- strain
- sow
- cordyceps
- cordyceps sinensis
- days
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- Granted
Links
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 66
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- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 claims abstract description 16
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims abstract description 8
- 229960005309 estradiol Drugs 0.000 claims abstract description 8
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- 239000000186 progesterone Substances 0.000 claims abstract description 8
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- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 claims description 9
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of method for improving sow reproductive performance, this method includes feeding sow using Cordyceps strain fermentation object as feed addictive.Present invention discover that sow is fed using Cordyceps strain fermentation object, can significantly improve sow sow number born per litter, the number of live births, piglet come into being counterpoise, 21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 ages in days wean nest counterpoise, shorten the inter oestrual period number of days of sow, the expression of oestrus of sow rate, estradiol and progesterone is improved simultaneously, and then realizes the raising of sow reproductive performance.
Description
Technical field
The present invention relates to livestock and poultry cultivation technical fields, and in particular to a kind of method for improving sow reproductive performance.
Background technology
The tool of production of the sow as pig farm, feeding objective are to improve insemination of sows rate, improve the strong young number of production, reduce weak son
Rate and raising sow in lactation milk water quality, so as to improve weaned piglet weight, reduce death and culling rate.Pig farm is female by improving
Pig production performance, allows sow maximizing the benefits, and then realizes increasing substantially for pig farm whole synthesis benefit.
However, in pig breeding industry, the effective nutriment intake deficiency of milking sow causes sow reproductive performance decline to be
The a great problem that pig farm faces is mainly reflected in lactation amount deficiency, influences the survival rate of piglet during the growth and lactation of piglet;
On the other hand, lose flesh between sows in lactation serious, cause after wean cannot heat in time or even out of heat, sow disease resistance compared with
Low, the phenomenon that postnatal care is not in place, and breeding rate is relatively low is also commonplace.
In actual production process, due to a large amount of uses of antibiotic, sow autoimmunity and disease resistance is caused to weaken,
On the one hand sow service life can be caused to shorten, on the other hand also results in sow reproductive performance decline, show as producing strong young number
It reduces, weak son's rate and still birth rate raising and breeding rate reduce.Therefore, sow reproductive performance how is improved, reduces piglet production
Cost, it has also become the key point of pig farm final and decisive juncture.
Cordyceps sinensis is the rare fungi-medicine in China and advanced tonic, before the Ming Dynasty of China, it is known that cordyceps sinensis is applied in road;
Since mid-Ming Dynasty, cordyceps sinensis is just liked by the Southeast Asia people, enjoys a very good reputation in the international market.
Sensu lato cordyceps sinensis refers to that entomogenous fungi infects the fungus sporophore (son formed after insect or arthropod
Seat) and the complex of insect or arthropod.There are about more than 400 to plant for Cordyceps sinensis fungus, wherein most rare is cordyceps sinensis
(Cordyceps sinensis) is that the linear Cordyceps sinensis fungus of Clavicipitaceae (Ophiocordyceps) parasitizes bat moth category
The complex formed in the larva body of (Hepialus spp.) is the endemic species of Qinghai-Tibet high altitude localities, referred to as " worm
Grass " is the rare Chinese medicine of Chinese tradition.
At present, in the world it has been found that the cordyceps sinensis for belonging to " Cordyceps " has as many as 350 kinds, wherein having been sent out in China
It now crosses more than 60 to plant, includes cordyceps sinensis (C.sinensis), northern Chinese caterpillar Fungus (C.militaris), agglomerate cordyceps sinensis
(C.ophioglossoides), Cordyceps hawkesii Gary (C.hawkesii), Cordyceps gunnii (Berk.) Berk (C.gunnii), coral cordyceps sinensis
(martialis), sickle shaped cordyceps sinensis (C.falcata), mountain Tai aweto (C.taishanensis), Shanxi cordyceps sinensis
(C.shanxiensis), liangshan cordyceps herb (C.liangshanensis), Xinjiang cordyceps sinensis (C.gracilis), incense stick cordycep
(C.barnesii), cicada fungus (C.sobolifera) etc..
Now, it has been found that cordyceps sinensis it is various in style and most cordyceps sinensis plays the role of very strong raising immunocompetence.It is logical
Above-mentioned effect is crossed, researchers successively successfully develop:(1) paecilomyces hepiall chen (1987 list), by from Cordyceps of Qinghai Province
In separating obtained Paecilomyces hepiali chen (Paecilomyces hepiali) Cs-4 bacterial strains be prepared;(2) Bailing capsule, with
A kind of fungi Hirsutella sinensis (also known as Chinese synnema spore (Synnematum sinense that Shen Nanying etc. is isolated in nineteen eighty-three
Y in et Shen sp.now), aweto-cephalosporin, Hirsutella hapial) it produces to obtain for strain;(3) zhiling capsules are used
The mycelium that mortierella sp (Mortierella sp) fermentation for isolating and purifying out from cordyceps sinensis obtains is made;(4) calming heart
Health-care capsule, by Clavicipitaceae fungi cordyceps sinensis cephalo (Cephalosporium sinensis isolated in fresh cordyceps sinensis
Chen.sp.nov) through obtained by liquid deep layer fermenting;(5) Xinganbao Jrao nang was isolated by 1986 in cordyceps sinensis fresh specimens
The caterpillar fungus cephalosporin come is made, which is a kind of fungi autoeciousness bacterium Gliocladiumroseum [Gliocladium roseum
(Link)Bain]。
In addition, application of the worm grass product on animal also obtains some achievements.Mortierella Diding culture has a variety of lifes
Object function, such as:Wei Jianzhong etc. is in document《Mortierella Diding culture is to the shadow of child care weaned piglets and immune level
It rings》In disclose mortierella Diding culture and can improve the production performance and immune level of child care piglet;And Li Yang etc. is by document
《Effects of acremonium terricola culture on growth performance,antioxidant
status and immune functions in weaned calves》、《Effects of acremonium
terricola culture supplementation on apparent digestibility,rumen
fermentation,and blood parameters in dairy cows》It is published in respectively《Livestock Science》
With《Animal Feed Science and Technology》In, mainly elaborate that Cordyceps strain fermentation object grows calf
Influence in terms of performance, cow in milk production performance and immunity.Application publication number is the application for a patent for invention of CN 101797014A
A kind of production method of nutritive eggs with low cholesterol and products thereof is elaborated in document;Application publication number is CN 104472904A
Application for a patent for invention document in elaborate that Cordyceps strain fermentation object is improving cow's milk flavor substance, vitamin or required amino
Application in acid content, application publication number are to elaborate Cordyceps strain in the application for a patent for invention document of CN 104522313A
Application of the fermentate in Methods of Milk Somatic Cell Images quantity is reduced.
However, there is presently no the reports applied in terms of sow reproductive performance is improved about cordyceps sinensis.
Invention content
The present invention provides a kind of methods for improving sow reproductive performance, and this method is using Cordyceps strain fermentation object as feeding
Feed additives feed sow, can significantly improve sow sow number born per litter, the number of live births, piglet come into being counterpoise,
21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 ages in days wean nest counterpoise shorten the inter oestrual period number of days of sow,
The expression of oestrus of sow rate, estradiol and progesterone is improved simultaneously, and then realizes the raising of sow reproductive performance.
Particular content is as follows:
A kind of method for improving sow reproductive performance, including:It is fed using Cordyceps strain fermentation object as feed addictive
Sow.
Cordyceps strain fermentation object of the present invention is obtained by Cordyceps sinensis fungus after liquid fermentation or solid fermentation;The worm
It can be liquid fermentation bacterium solution or the bacterium powder being prepared into using liquid fermentation solid content that grass, which belongs to strain fermentation object, also
It can be the solid fermentation object further prepared using liquid fermentation bacterium solution.
Meaning Cordyceps sinensis fungus of the invention refers to isolated parasitical fungi from cordyceps sinensis.
Further, the Cordyceps sinensis fungus is cordyceps sinensis (C.sinensis) strain, northern Chinese caterpillar Fungus
(C.militaris) strain, agglomerate cordyceps sinensis (C.ophioglossoides) strain, Cordyceps hawkesii Gary (C.hawkesii) strain,
Cordyceps gunnii (Berk.) Berk (C.gunnii) strain, coral cordyceps sinensis (C.martialis) strain, sickle shaped cordyceps sinensis (C.falcata) strain, Thailand
Mountain cordyceps sinensis (C.taishanensis) strain, Shanxi cordyceps sinensis (C.shanxiensis) strain, liangshan cordyceps herb
(C.liangshanensis) strain, Xinjiang cordyceps sinensis (C.gracilis) strain, incense stick cordycep (C.barnesii) strain, cicada fungus
(C.sobolifera) at least one of strain.
Further, the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola), Hirsutella sinensis
(Synnematium sinense sp.), pupa draw up mould (Paecilomyces militaris), Paecilomyces hawkesii
(Paecilomyces hawkesii), Paraisaria mould (Paraisaria sp.), false truffle (Elaphomyces
Granulatus), at least one of cicada muscardine (Beauveria sobolifera).
Experiments have shown that compared with the fermentate of other Cordyceps sinensis fungus, mortierella Diding (Acremonium terricola)
The reproductive performance of sow can more effectively be improved;Therefore preferably, the Cordyceps sinensis fungus is mortierella Diding
(Acremonium terricola)。
Further, the Cordyceps sinensis fungus is mortierella Diding (Acremonium terricola) MKL18;It is true with this
Bacterium can significantly improve the reproductive performance of sow as feed addictive.
The liquid fermentation includes:(1) Cordyceps sinensis fungus is activated;(2) Cordyceps sinensis fungus is taken to be inoculated in seed culture
In base, seed culture is carried out;It is forwarded to and expands in culture medium again, be enlarged culture, obtain liquid fermentation bacterium solution.
The solid fermentation includes:After liquid fermentation bacterium solution is taken to be mixed with solid medium, fermentation obtains solid culture
Object.
The liquid fermentation medium and solid fermentation culture medium that the present invention uses are conventional medium, without special Chinese medicine
Ingredients are waited, Cordyceps sinensis fungus and its fermentating metabolism product are only contained in the fermentate of acquisition, the present invention is to utilize Cordyceps sinensis fungus
The tunning that itself fermentation generates realizes the raising of sow reproductive performance.
Above-mentioned conventional medium includes nitrogen source, carbon source and trace element;Wherein, preferably, nitrogen source is dried silkworm chrysalis meal, albumen
At least one of peptone, dusty yeast and analysis for soybean powder, carbon source be white granulated sugar, analysis for soybean powder, corn flour, malt flour and rice meal in extremely
Few one kind, trace element are MgSO4、KH2PO4、NaH2PO4And VBAt least one of.
Further, it is found through experiment that, the ingredient of Cordyceps strain fermentation object that the fermentation of various conventional culture medium obtains contains
Amount is different, and different component contents can influence the reproductive performance of sow.
Preferably, in the culture medium of liquid fermentation, the nitrogen source includes the dried silkworm chrysalis meal and 5~20g/L of 3~30g/L
Peptone (on the basis of culture medium).
Further, the liquid fermentation includes seed culture and expands to cultivate;In terms of 1L culture mediums, the seed training
It supports and the culture medium of expansion culture is:20~60g white granulated sugars, 3~30g dried silkworm chrysalis meals, 5~30g analysis for soybean powder, 5~20g peptones, 3
~40g dusty yeasts, 0.01~1.0g MgSO4, 0.01~1.0g KH2PO4。
Preferably, in terms of the quality of culture medium, the culture medium of the solid fermentation includes:Corn flour 30~80%, cotton
Seed powder 5~20%, wheat wheat-middlings 2~15%, dregs of beans 15~40%, wheat bran 5~20%, magnesium sulfate 0.01~1.0%, zinc sulfate
0.01~1.0%, potassium dihydrogen phosphate 0.01~1.0%, sodium dihydrogen phosphate 0.01~1.0%.
Further, Cordyceps sinensis polysaccharide, adenosine and ergosterol are contained in the Cordyceps strain fermentation object.
The results showed Cordyceps sinensis polysaccharide, adenosine and ergosterol that different types of Cordyceps strain fermentation object obtains
Content is different, and then influences the reproductive performance of sow.
In terms of mass fraction, containing 0.5~40% Cordyceps sinensis polysaccharide in the Cordyceps strain fermentation object, 0.005~0.4%
Adenosine and 0.001~0.6% ergosterol.Further, it is more containing 2~10% cordyceps sinensis in the Cordyceps strain fermentation object
Sugar, 0.005~0.2% adenosine and 0.001~0.3% ergosterol.
Further, sow of the sow for the gestational period and nursing period;Further, the sow is female for adult production
Pig.
Index for characterizing the sow reproductive performance includes:The number born per litter of sow, the number of live births, piglet
Nascent counterpoise, 21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 ages in days wean nest counterpoise, inter oestrual period number of days, hair
The content of feelings rate and estradiol and progesterone.
Further, the Cordyceps strain fermentation object, which has, improves sow number born per litter, the number of live births, piglet
Nascent counterpoise, 21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 ages in days wean nest counterpoise, shorten inter oestrual period day
Number improves Oestrus rate and the function of estradiol and progesterone content.
Preferably, after the Cordyceps strain fermentation object is mixed with basal diet, then feed sow;With the basis
The dry weight meter of daily ration, the additive amount of the Cordyceps strain fermentation object is 0.5~3%.
In the range of the scale of feeding, sow can reach best reproductive performance;Due to the Cordyceps strain fermentation of the present invention
Object is to sow and without any side effects, so any adverse effect will not be generated in itself to sow by increasing scale of feeding.
Cordyceps strain fermentation object of the present invention can also be mixed separately as feed addictive with other feed addictives
It uses;It is better if coordinating rational basal diet formula.
Compared with prior art, beneficial effects of the present invention are:
Present invention discover that feeding sow using Cordyceps strain fermentation object, the sow nest that can significantly improve sow is farrowed
Number, the number of live births, piglet come into being counterpoise, 21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 ages in days wean nest
Counterpoise, shortens the inter oestrual period number of days of sow, while improves the expression of oestrus of sow rate, estradiol and progesterone, Jin Ershi
The raising of existing sow reproductive performance.
Specific embodiment
The present invention is described in further detail With reference to embodiment.
Embodiment 1
1st, zymocyte liquid
(1) Cordyceps gunnii (Berk.) Berk strain:Mortierella Diding (Acremonium terricola) MKL18 (is purchased from Hefei Mai Keluo
Bioengineering Co., Ltd);
(2) seed culture:
The culture medium of seed culture is:600g white granulated sugars, 300g dried silkworm chrysalis meals, 150g peptones, 200g analysis for soybean powder, 100g ferment
Female powder, 10g MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
The condition of seed fermentation is:Temperature is 25 DEG C, and ventilatory capacity is 1.00L/ (Lmin), and inoculum concentration is 5% (quality point
Number), 30L airlift fermentors liquid amount 67%, fermentation time is for 24 hours.
(3) expand culture:
Expanding the culture medium cultivated is:6000g white granulated sugars, 3000g dried silkworm chrysalis meals, 1500g peptones, 2000g analysis for soybean powder,
1000g dusty yeasts, 100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
Expanding the condition cultivated is:Temperature is 25 DEG C, and ventilatory capacity is 1.00L/ (Lmin), and inoculum concentration is 10% (quality
Score), 300L airlift fermentors liquid amount 67%, fermentation time is for 24 hours.
After the completion of seed culture and expansion culture, mortierella Diding bacterium solution is obtained.
2nd, zymophyte powder
(1) mortierella Diding bacterium solution obtained as process object, pumps out bacterium solution from fermentation tank using in the present embodiment 1, from
The heart detaches, and obtains mycelia and solid content;
(2) above-mentioned mycelia and solid content are collected, heated-air drying is down to 8wt% hereinafter, crushing pack to moisture at 45 DEG C,
Sealing, is protected from light storage, obtains zymophyte powder.
3rd, solid culture
(1) by (inoculum concentration 15%, matter after zymocyte liquid obtained in the present embodiment 1 and solid medium stirring inoculation
Measure score), tray top fermentation, material thickness 3cm are laid in, constant temperature is 25 DEG C, humidity 70%, and fermentation time is
120h obtains solid culture;
Wherein, the culture medium of solid fermentation is:Corn flour 45%, cotton seed meal 5%, wheat wheat-middlings 4%, dregs of beans 30%, wheat
Bran 15.8%, magnesium sulfate 0.05%, zinc sulfate 0.05%, potassium dihydrogen phosphate 0.05%, sodium dihydrogen phosphate 0.05%;Natural pH,
121 DEG C of steam sterilizing 1h.
(2) solid culture is collected, heated-air drying is down to 8wt% hereinafter, crushing pack to moisture at 60 DEG C, sealing,
It is protected from light storage.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 1.
Embodiment 2
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains
Obtain zymocyte liquid, zymophyte powder and solid culture;In addition to the culture medium of the liquid fermentation of use is different from embodiment 1,
Remaining content is same as Example 1.
The culture medium of seed culture is:600g white granulated sugars, 550g dusty yeasts, 200g analysis for soybean powder, 10g MgSO4,
10gKH2PO4, it is dissolved in 20L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is:6000g white granulated sugars, 5500g dusty yeasts, 2000g analysis for soybean powder, 100g MgSO4,
100g KH2PO4, it is dissolved in 200L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 2.
Compound mensuration result in 2 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Embodiment 3
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains
Obtain zymocyte liquid, zymophyte powder and solid culture;In addition to the culture medium of the liquid fermentation of use is different from embodiment 1,
Remaining content is same as Example 1.
The culture medium of seed culture is:600g white granulated sugars, 400g dusty yeasts, 200g analysis for soybean powder, 150g peptones, 10g
MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is:6000g white granulated sugars, 4000g dusty yeasts, 2000g analysis for soybean powder, 1500g peptones,
100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 3.
Compound mensuration result in 3 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Embodiment 4
The present embodiment ferments using mortierella Diding (Acremonium terricola) MKL18 as fermentation object, obtains
Obtain zymocyte liquid, zymophyte powder and solid culture;In addition to the culture medium of the liquid fermentation of use is different from embodiment 1,
Remaining content is same as Example 1.
The culture medium of seed culture is:600g white granulated sugars, 250g dusty yeasts, 200g analysis for soybean powder, 300g dried silkworm chrysalis meals, 10g
MgSO4, 10g KH2PO4, it is dissolved in 20L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
Expanding the culture medium cultivated is:6000g white granulated sugars, 2500g dusty yeasts, 2000g analysis for soybean powder, 3000g dried silkworm chrysalis meals,
100g MgSO4, 100g KH2PO4, it is dissolved in 200L water, natural pH;121 DEG C of high pressure steam sterilization 30min.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 4.
Compound mensuration result in 4 mortierella Diding zymocyte liquid of table, zymophyte powder and solid culture
Component (Cordyceps sinensis polysaccharide, adenosine by table 1~4 it is found that different culture media ferments in the mortierella Diding fermentate obtained
With ergosterol) content is different, wherein, Cordyceps sinensis polysaccharide, gland in the fermentate obtained using the liquid fermentation medium of embodiment 1
The equal highest of the content of glycosides and ergosterol.
Embodiment 5
The present embodiment draws up mould (Paecilomyces militaris) MKL32 with pupa and (is purchased from Anhui nova biology
Engineering Co., Ltd) it ferments as fermentation object, obtain zymocyte liquid, zymophyte powder and solid culture;Preparation side
Method is same as Example 1.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 5.
5 pupa of table draws up the compound mensuration result in mould zymocyte liquid, zymophyte powder and solid culture
Embodiment 6
The present embodiment (is purchased from Anhui nova biology with Hirsutella sinensis (Synnematium sinense sp.) MKL25
Engineering Co., Ltd) it ferments as fermentation object, obtain zymocyte liquid, zymophyte powder and solid culture;Preparation side
Method is same as Example 1.
The component in zymocyte liquid, zymophyte powder and solid culture that the present embodiment prepares is measured, as a result such as
Shown in table 6.
Compound mensuration result in 6 Hirsutella sinensis zymocyte liquid of table, zymophyte powder and solid culture
Component (Cordyceps sinensis polysaccharide, adenosine and ergot steroid by table 1,5 and 6 it is found that different strain ferments in the fermentate obtained
Alcohol) content is also different;Wherein, the fermentation obtained using the mortierella Diding of embodiment 1 (Acremonium terricola) MKL18
The equal highest of the content of Cordyceps sinensis polysaccharide, adenosine and ergosterol in object.
Embodiment 7
1st, test process:
In the large-scale cultivation group pig farm in Jiangsu, using Cordyceps strain fermentation object prepared by Examples 1 to 6 as feeding
Feed additives carry out sow the observation experiment of 72 days by a definite date.
It is as follows to test particular content:
Antenatal 35 days in sow on-test, first 3 days are preliminary trial period, observe sow health status and feed intake;Nursing period
It is 21 days, the off-test in 10 days after sow is weaned.
Selection kind is consistent, is in a good state of health and 2-4 tires sow totally 140 that the expected date of childbirth is close, is randomly divided into 7 groups
(test group 1~6 and control group), every group of 2 repetitions each repeat 10 sows.Specially:
Control group:Basal diet is only fed, the composition of basal diet is shown in Table 7;
Test group:With embodiment 1 (test group 1), embodiment 2 (test group 2), embodiment 3 (test group 3), embodiment 4
The Cordyceps strain solid culture that (test group 4), embodiment 5 (test group 5) and embodiment 6 (test group 6) prepare is
Feed addictive is added in basal diet, obtained feed feeding sow;In terms of the quality of basal diet, within preliminary trial period,
The additive amount of each Cordyceps strain solid culture is 0.1%;Within experimental period, the addition of each Cordyceps strain solid culture
Measure is 0.2%.
7 basal diet of table forms and trophic level (air bells dry basis)
2nd, index determining and method
2.1 sow reproductive performance index determinings
Recorded after on-test number born per litter, the number of live births, piglet birth individual weight, the young number of 21 ages in days wean,
21 ages in days wean individual weight, calculate nascent counterpoise and nest counterpoise, 21 ages in days wean counterpoise and nest counterpoise respectively, observe sow 7d and
Heat situation in 10d records wean-inter oestrual period time, calculates Oestrus rate.
2.2 hormone serum levels measure
Night, which prohibits, on the day of wean raises, second day 8:30, it is each to repeat to choose 2 sows and each processing group selection 4
Sow carries out vena cava anterior blood sampling 10ml, injects in heparin sodium centrifuge tube, 3000r/min collects blood after centrifuging 15min after standing
Clearly, -20 DEG C of preservations of sample.Measure the content of estradiol (estradiol, E) and progesterone (progesterone, P) in serum, examination
Agent box is purchased from RD companies of the U.S., and step is operated to specifications.
3rd, result of the test
Concrete outcome is shown in Table 8.
The situation of sow reproductive performance in 8 different tests group of table
As shown in Table 8, compared with the control group, test group 1~7 be significantly increased the number born per litter of sow, nascent counterpoise,
Oestrus rate and wean 10d heats in 21 ages in days wean counterpoise, 21 ages in days wean nest counterpoise, wean-inter oestrual period number and wean 7d
Rate improves hormone secretion level in serum;Wherein, the improvement effect of test group 1 is best.Thus illustrate:Using different culture media
And different strain carry out the solid culture of fermentation acquisition in terms of the reproductive performance for improving sow on have differences, it is and above-mentioned
Difference be likely to be in fermentate constituent content difference (the especially content of Cordyceps sinensis polysaccharide, adenosine and ergosterol) made
Into.
Claims (9)
- A kind of 1. method for improving sow reproductive performance, which is characterized in that including:Add using Cordyceps strain fermentation object as feed Agent is added to feed sow.
- 2. the method as described in claim 1, which is characterized in that the Cordyceps strain fermentation object is by Cordyceps sinensis fungus through liquid It is obtained after fermentation or solid fermentation.
- 3. method as claimed in claim 2, which is characterized in that the Cordyceps sinensis fungus is cordyceps sinensis (C.sinensis) bacterium Kind, northern Chinese caterpillar Fungus (C.militaris) strain, agglomerate cordyceps sinensis (C.ophioglossoides) strain, Cordyceps hawkesii Gary (C.hawkesii) strain, Cordyceps gunnii (Berk.) Berk (C.gunnii) strain, coral cordyceps sinensis (C.martialis) strain, sickle shaped cordyceps sinensis (C.falcata) strain, mountain Tai aweto (C.taishanensis) strain, Shanxi cordyceps sinensis (C.shanxiensis) strain, the Liangshan Mountain Cordyceps sinensis (C.liangshanensis) strain, Xinjiang cordyceps sinensis (C.gracilis) strain, incense stick cordycep (C.barnesii) strain, At least one of cicada fungus (C.sobolifera) strain.
- 4. method as claimed in claim 3, which is characterized in that the Cordyceps sinensis fungus is mortierella Diding(Acremonium terricola), Hirsutella sinensis (Synnematium sinense sp.), pupa draw up mould (Paecilomyces militaris), Paecilomyces hawkesii (Paecilomyces hawkesii), Paraisaria are mould (Paraisaria sp.), false truffle (Elaphomyces granulatus), cicada muscardine (Beauveria At least one of sobolifera).
- 5. method as claimed in claim 3, which is characterized in that the Cordyceps sinensis fungus is mortierella Diding(Acremonium terricola)。
- 6. method as claimed in claim 2, which is characterized in that the culture medium of the liquid fermentation includes:Nitrogen source, carbon source and micro- Secondary element;Wherein, nitrogen source is at least one of for dried silkworm chrysalis meal, peptone, dusty yeast and analysis for soybean powder, carbon source is white granulated sugar, analysis for soybean powder, At least one of corn flour, malt flour and rice meal, trace element are MgSO4、KH2PO4、NaH2PO4And VBIn at least one Kind.
- 7. method as claimed in claim 2, which is characterized in that in terms of mass fraction, contain in the Cordyceps strain fermentation object There are 0.5~40% Cordyceps sinensis polysaccharide, 0.005~0.4% adenosine and 0.001~0.6% ergosterol.
- 8. the method as described in claim 1, which is characterized in that the index for characterizing the reproductive performance includes:Sow Number born per litter, the number of live births, piglet come into being counterpoise, 21 age in days number pigs weaneds, 21 age in days weanling pig counterpoises, 21 days Age wean nest counterpoise, inter oestrual period number of days, Oestrus rate and the content of estradiol and progesterone.
- 9. the method as described in claim 1, which is characterized in that mix the Cordyceps strain fermentation object with basal diet Afterwards, then sow is fed;In terms of the dry weight of the basal diet, the additive amount of the Cordyceps strain fermentation object is 0.005~1%.
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CN114747683A (en) * | 2022-04-25 | 2022-07-15 | 华南农业大学 | Feed for improving birth weight of piglets and reducing stillbirth rate |
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CN114747683A (en) * | 2022-04-25 | 2022-07-15 | 华南农业大学 | Feed for improving birth weight of piglets and reducing stillbirth rate |
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