CN113080323A - Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets - Google Patents
Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets Download PDFInfo
- Publication number
- CN113080323A CN113080323A CN202011445287.0A CN202011445287A CN113080323A CN 113080323 A CN113080323 A CN 113080323A CN 202011445287 A CN202011445287 A CN 202011445287A CN 113080323 A CN113080323 A CN 113080323A
- Authority
- CN
- China
- Prior art keywords
- piglets
- culture medium
- improved
- cordyceps
- fermentation liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 39
- 230000004151 fermentation Effects 0.000 title claims abstract description 39
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 230000036039 immunity Effects 0.000 title claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 241001327634 Agaricus blazei Species 0.000 claims abstract description 26
- 241000190633 Cordyceps Species 0.000 claims abstract description 25
- 241000222336 Ganoderma Species 0.000 claims abstract description 25
- 150000001875 compounds Chemical class 0.000 claims abstract description 24
- 239000007787 solid Substances 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000002131 composite material Substances 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 235000010585 Ammi visnaga Nutrition 0.000 claims description 3
- 244000153158 Ammi visnaga Species 0.000 claims description 3
- 206010011732 Cyst Diseases 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 claims description 3
- 208000031513 cyst Diseases 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 abstract description 8
- 239000003651 drinking water Substances 0.000 abstract description 5
- 235000020188 drinking water Nutrition 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 230000003053 immunization Effects 0.000 abstract description 5
- 238000002649 immunization Methods 0.000 abstract description 5
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 abstract description 3
- 230000010307 cell transformation Effects 0.000 abstract description 3
- 230000001413 cellular effect Effects 0.000 abstract description 3
- 210000002865 immune cell Anatomy 0.000 abstract description 3
- 230000001900 immune effect Effects 0.000 abstract description 3
- 230000036737 immune function Effects 0.000 abstract description 3
- 210000004698 lymphocyte Anatomy 0.000 abstract description 3
- 210000000056 organ Anatomy 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 230000009466 transformation Effects 0.000 abstract description 3
- 229960005486 vaccine Drugs 0.000 abstract description 3
- 241000282887 Suidae Species 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 241000282898 Sus scrofa Species 0.000 description 5
- 230000010100 anticoagulation Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 206010008631 Cholera Diseases 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940030156 cell vaccine Drugs 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 240000008397 Ganoderma lucidum Species 0.000 description 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets, which comprises the following steps: the method comprises the following steps: separating to obtain Cordyceps, Ganoderma and Agaricus blazei composite strain; step two: preparing an improved PDA solid culture medium and an improved PD culture medium; step three: the improved solid PDA culture medium is heated to melt, after the temperature is cooled, the improved solid PDA culture medium is poured into a culture dish under the aseptic condition, and the culture medium is poured for standby after being cooled and solidified, wherein the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor is added into drinking water fed after weaning of piglets, so that the lymphocyte transformation of the piglets can be obviously enhanced, the antibody content in serum and the immune function of organisms are improved, the antibody titer after the immunization of vaccines is improved, the immune organ cell transformation of the weaning piglets can be obviously enhanced, the cellular immune effect is promoted, the arbor feeding capability of immune cells to bacteria and viruses is strengthened, the disease resistance is strengthened, the effect of promoting the elongation can be obviously played, the immunity of the piglets can be improved, and the problem of multiple diseases of the piglets is fundamentally solved.
Description
Technical Field
The invention relates to the technical field of preparation of cordyceps sinensis fermentation liquor, and particularly relates to a preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets.
Background
The pig is vertebrate, mammal, livestock, and ancient omnivorous mammal. It is divided into domestic pig and wild pig. Pigs are now generally considered an acronym for porcine. The physical characteristics of pigs are different according to different breeds. But usually the major physical characteristics are big ear, long head, straight nose, narrow back and waist. The hair is coarse and hard, and is classified into white, pink, black, brown and colored domestic pigs according to varieties, the domestic pigs are subspecies formed after wild pigs are domesticated by human beings, the bucktooth is shorter than the wild pigs and is one of human domestic animals, and the domestic pigs generally refer to the breeded and edible pig species of human beings.
The feeding of the existing pigs is critical in the breeding process, the existing weaned piglets are prone to being ill, the situation that the piglets die often occurs due to unreasonable feeding in the breeding process, and the situation that the piglets are ill again often needs to be intervened through medical treatment means in the feeding process, but the disease occurrence situation cannot be fundamentally solved, and the immunity of the piglets cannot be improved, so that the preparation method of the cordyceps sinensis fermentation liquor for improving the immunity of the piglets is provided.
Disclosure of Invention
The invention aims to provide a preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets, and solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets comprises the following steps:
the method comprises the following steps: separating to obtain Cordyceps, Ganoderma and Agaricus blazei composite strain;
step two: preparing an improved PDA solid culture medium and an improved PD culture medium;
step three: heating and melting the improved solid PDA culture medium, cooling, pouring into a culture dish under the aseptic condition, cooling and solidifying the culture medium, and pouring for later use;
step four: respectively inoculating the cordyceps, lucid ganoderma and agaricus blazei murill compound strains on an improved culture medium under aseptic operation, performing illumination culture in a constant-temperature incubator for 15-20 days, and placing in a refrigerator at 4 ℃ for later use when the strains grow to be close to the edge of a culture dish;
step five: taking out the culture medium full of strains from a refrigerator, picking a small amount of pink conidia by using a sterilized toothpick under aseptic condition, and inoculating the conidia into a conical flask filled with a sterilized improved PD liquid culture medium;
step six: placing inoculated conical flasks containing different mating types in a shaking cabinet for shaking culture, and then carrying out shaking culture at constant temperature for 3-4 d to obtain a small amount of seed bacteria suspension containing a plurality of mycelial pellets of the small rice grains;
step seven: transferring the seed bacteria suspension to a fermentation tank for fermentation to obtain the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor.
As a preferred embodiment of the present invention, the separation to obtain the cordyceps, ganoderma and agaricus blazei murill compound strain: in a laboratory, performing single-seed cyst spore separation on a tested cordyceps strain, a tested ganoderma strain and a tested agaricus blazei murill strain, and then obtaining the cordyceps, ganoderma and agaricus blazei murill compound strain through molecular biological identification and artificial screening.
As a preferred embodiment of the present invention, the preparation of the modified solid medium is prepared as follows: 200g of potato, 20g of glucose, 3g of peptone, 16g of agar, 2g of monopotassium phosphate, 1g of magnesium sulfate heptahydrate and compound VB25mg of the above-mentioned components were mixed and the mixture was made up to 1000mL with water, and the pH was 6.5-7.
In a preferred embodiment of the present invention, the modified PD medium is prepared by mixing potato (200 g), glucose (20 g), peptone (3 g), potassium dihydrogen phosphate (2 g), magnesium sulfate (1 g), and Complex VBMixing 25mg of the above components, and adding water until the pH value is 6.5-7.0.
In the sixth step, the rotation speed of the shaking culture is 170r/min, and the temperature is 20-22 ℃.
As a preferred embodiment of the present invention: the temperature condition in the fourth step is 20-22 ℃.
In a preferred embodiment of the invention, the fermentation conditions in the seventh step are temperature of 20-25 ℃, pH value of 6.0-6.5 and ventilation volume of 1 mL/min.
In a preferred embodiment of the present invention, the temperature after cooling in the third step is 55 to 60 ℃.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor is added into drinking water fed after the piglets are weaned, so that the lymphocyte transformation of the piglets can be obviously enhanced, the antibody content in serum and the immune function of organisms are improved, the antibody titer after the vaccine immunization is improved, the immune organ cell transformation of the weaned piglets can be obviously enhanced, the cellular immune effect is promoted, the arbor-feeding capability of immune cells to bacteria and viruses is enhanced, the disease resistance is enhanced, the effect of promoting elongation can be obviously played, the immunity of the piglets can be enhanced, and the problem of multiple diseases of the piglets is fundamentally solved.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
The invention provides a technical scheme that: a preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets comprises the following steps:
the method comprises the following steps: separating to obtain Cordyceps, Ganoderma and Agaricus blazei composite strain;
step two: preparing an improved PDA solid culture medium and an improved PD culture medium;
step three: heating and melting the improved solid PDA culture medium, cooling, pouring into a culture dish under the aseptic condition, cooling and solidifying the culture medium, and pouring for later use;
step four: respectively inoculating the cordyceps, lucid ganoderma and agaricus blazei murill compound strains on an improved culture medium under aseptic operation, performing illumination culture in a constant-temperature incubator for 15-20 days, and placing in a refrigerator at 4 ℃ for later use when the strains grow to be close to the edge of a culture dish;
step five: taking out the culture medium full of strains from a refrigerator, picking a small amount of pink conidia by using a sterilized toothpick under aseptic condition, and inoculating the conidia into a conical flask filled with a sterilized improved PD liquid culture medium;
step six: placing inoculated conical flasks containing different mating types in a shaking cabinet for shaking culture, and then carrying out shaking culture at constant temperature for 3-4 d to obtain a small amount of seed bacteria suspension containing a plurality of mycelial pellets of the small rice grains;
step seven: transferring the seed bacteria suspension to a fermentation tank for fermentation to obtain the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor.
Further, the separation is carried out to obtain the cordyceps, lucid ganoderma and agaricus blazei murill compound strain: in a laboratory, performing single-seed cyst spore separation on a tested cordyceps strain, a tested ganoderma strain and a tested agaricus blazei murill strain, and then obtaining the cordyceps, ganoderma and agaricus blazei murill compound strain through molecular biological identification and artificial screening.
Further, the preparation of the improved solid medium is prepared as follows: 200g of potato, 20g of glucose, 3g of peptone, 16g of agar, 2g of monopotassium phosphate, 1g of magnesium sulfate heptahydrate and compound VB25mg of the above-mentioned components were mixed and the mixture was made up to 1000mL with water, and the pH was 6.5-7.
Further, the improved PD medium is prepared by mixing potato 200g, glucose 20g, peptone 3g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, and compound VBMixing 25mg of the above components, and adding water until the pH value is 6.5-7.0.
Further, in the sixth step, the rotation speed of the shaking culture is 170r/min, and the temperature condition is 20-22 ℃.
Further, the temperature condition in the fourth step is 20-22 ℃.
Further, the fermentation conditions in the seventh step are that the temperature is 20-25 ℃, the pH value is 6.0-6.5, and the ventilation volume is 1 mL/min.
Further, the temperature condition after cooling in the third step is 55-60 ℃
The method comprises the following steps: randomly selecting 20 weaned piglets as experimental objects, feeding the weaned piglets with normal feed and drinking water, and carrying out closed feeding on the restructured piglets with a feeding period of 30 days;
step two: sampling 10 piglets at 14 days and 28 days, and injecting the sampled blood into a vacuum EDTA anticoagulation tube and a vacuum biochemical tube respectively to prepare anticoagulation blood and serum;
step three: all experimental piglets were immunized with an equal dose of hog cholera cell vaccine at 25 days of age. Collecting blood for 10 blood samples of each treatment group at 7, 14, 21 and 28 days after immunization, collecting blood with vacuum biochemical tube anterior cavity vein, separating serum, and freezing and storing with-2C;
step four: the pigs and the rest of the feed in each test group were weighed at the beginning of the test, at 7 days, 14 days, 21 days and 28 days after the test, and the average daily gain, average daily feed intake and feed-weight ratio of the pigs in each test group were calculated.
Example two
The method comprises the following steps: randomly selecting 20 weaned piglets as experimental objects and normal feed, adding 100mg/100L of cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor on the basis of normal drinking water, and carrying out closed feeding on the reorganized piglets for 30 days;
step two: sampling 10 piglets at 14 days and 28 days, and injecting the sampled blood into a vacuum EDTA anticoagulation tube and a vacuum biochemical tube respectively to prepare anticoagulation blood and serum;
step three: all experimental piglets were immunized with an equal dose of hog cholera cell vaccine at 25 days of age. Collecting blood for 10 blood samples of each treatment group at 7, 14, 21 and 28 days after immunization, collecting blood with vacuum biochemical tube anterior cavity vein, separating serum, and freezing and storing with-2C;
step four: the pigs and the rest of the feed in each test group were weighed at the beginning of the test, at 7 days, 14 days, 21 days and 28 days after the test, and the average daily gain, average daily feed intake and feed-weight ratio of the pigs in each test group were calculated.
The effect of the compound fermentation broth of cordyceps, lucid ganoderma and agaricus blazei murill on the conversion rate of peripheral blood lymphocytes of piglets is as follows:
the influence of the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation broth obtained according to the experiment on the number of peripheral blood lymphocyte subsets of weaned piglets is as follows:
according to the experiment, the influence (blocking rate) of the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor on the swine fever antibody in the serum of the weaned piglet is as follows:
according to the experiment, the influence of the cordyceps sinensis fermentation liquor on the growth performance of the weaned piglets is as follows:
in summary, the second embodiment with the addition of the cordyceps sinensis fermentation broth is obtained by referring to the comparison of the experimental data, and is a preferred embodiment, the compound cordyceps sinensis, ganoderma lucidum and agaricus blazei murill fermentation broth is added into drinking water fed after weaning of piglets, so that the lymphocyte transformation of the piglets can be obviously enhanced, the antibody content in serum and the immune function of organisms can be improved, the antibody titer after the immunization of vaccines can be improved, the immune organ cell transformation of the weaned piglets can be obviously enhanced, the cellular immune effect can be promoted, the arbor-feeding ability of immune cells to bacteria and viruses can be enhanced, the disease resistance can be enhanced, the effect of promoting elongation can be obviously achieved, the immunity of the piglets can be enhanced, and the problem of multiple diseases of the piglets can be fundamentally solved.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. A preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets is characterized by comprising the following steps: the preparation method of the cordyceps sinensis fermentation liquor comprises the following steps:
the method comprises the following steps: separating to obtain Cordyceps, Ganoderma and Agaricus blazei composite strain;
step two: preparing an improved PDA solid culture medium and an improved PD culture medium;
step three: heating and melting the improved solid PDA culture medium, cooling, pouring into a culture dish under the aseptic condition, cooling and solidifying the culture medium, and pouring for later use;
step four: respectively inoculating the cordyceps, lucid ganoderma and agaricus blazei murill compound strains on an improved culture medium under aseptic operation, performing illumination culture in a constant-temperature incubator for 15-20 days, and placing in a refrigerator at 4 ℃ for later use when the strains grow to be close to the edge of a culture dish;
step five: taking out the culture medium full of strains from a refrigerator, picking a small amount of pink conidia by using a sterilized toothpick under aseptic condition, and inoculating the conidia into a conical flask filled with a sterilized improved PD liquid culture medium;
step six: placing inoculated conical flasks containing different mating types in a shaking cabinet for shaking culture, and then carrying out shaking culture at constant temperature for 3-4 d to obtain a small amount of seed bacteria suspension containing a plurality of mycelial pellets of the small rice grains;
step seven: transferring the seed bacteria suspension to a fermentation tank for fermentation to obtain the cordyceps, lucid ganoderma and agaricus blazei murill compound fermentation liquor.
2. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: the compound strain of cordyceps, lucid ganoderma and agaricus blazei murill is obtained by separation: in a laboratory, performing single-seed cyst spore separation on a tested cordyceps strain, a tested ganoderma strain and a tested agaricus blazei murill strain, and then obtaining the cordyceps, ganoderma and agaricus blazei murill compound strain through molecular biological identification and artificial screening.
3. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: the preparation of the improved solid culture medium is as follows: 200g of potato, 20g of glucose, 3g of peptone, 16g of agar, 2g of monopotassium phosphate, 1g of magnesium sulfate heptahydrate and compound VB25mg of the above-mentioned components were mixed and the mixture was made up to 1000mL with water, and the pH was 6.5-7.
4. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: the improved PD culture medium is prepared from potato 200g, glucose 20g, peptone 3g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, and compound VBMixing 25mg of the above components, and adding water until the pH value is 6.5-7.0.
5. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: in the sixth step, the rotation speed of the shaking culture is 170r/min, and the temperature condition is 20-22 ℃.
6. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: the temperature condition in the fourth step is 20-22 ℃.
7. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: and the fermentation conditions in the seventh step are that the temperature is 20-25 ℃, the pH value is 6.0-6.5, and the ventilation volume is 1 mL/min.
8. The preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets according to claim 1, which is characterized by comprising the following steps: the temperature condition after cooling in the third step is 55-60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011445287.0A CN113080323A (en) | 2020-12-08 | 2020-12-08 | Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011445287.0A CN113080323A (en) | 2020-12-08 | 2020-12-08 | Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113080323A true CN113080323A (en) | 2021-07-09 |
Family
ID=76663709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011445287.0A Pending CN113080323A (en) | 2020-12-08 | 2020-12-08 | Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113080323A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194674A (en) * | 2007-12-29 | 2008-06-11 | 福建省农业科学院畜牧兽医研究所 | Feed additive for improving pigling intestinal canal function and immunity function, and use method thereof |
| CN101371683A (en) * | 2008-10-10 | 2009-02-25 | 李绩 | Agaricus blazei feedstuff additive product and method for producing the same and application |
| CN105002095A (en) * | 2014-04-15 | 2015-10-28 | 大自然生机股份有限公司 | Composite ganoderma strain co-culture method and applications thereof |
| CN107712268A (en) * | 2017-10-11 | 2018-02-23 | 浙江大学 | Effectively improve preparation method and the application of the special feed for rex rabbit of pup survival rate |
| CN108142359A (en) * | 2017-12-29 | 2018-06-12 | 合肥迈可罗生物工程有限公司 | A kind of method for improving sow reproductive performance |
| CN110982868A (en) * | 2019-11-29 | 2020-04-10 | 贵州中医药大学 | Co-culture method for improving triterpene content of ganoderma lucidum and application |
-
2020
- 2020-12-08 CN CN202011445287.0A patent/CN113080323A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194674A (en) * | 2007-12-29 | 2008-06-11 | 福建省农业科学院畜牧兽医研究所 | Feed additive for improving pigling intestinal canal function and immunity function, and use method thereof |
| CN101371683A (en) * | 2008-10-10 | 2009-02-25 | 李绩 | Agaricus blazei feedstuff additive product and method for producing the same and application |
| CN105002095A (en) * | 2014-04-15 | 2015-10-28 | 大自然生机股份有限公司 | Composite ganoderma strain co-culture method and applications thereof |
| CN107712268A (en) * | 2017-10-11 | 2018-02-23 | 浙江大学 | Effectively improve preparation method and the application of the special feed for rex rabbit of pup survival rate |
| CN108142359A (en) * | 2017-12-29 | 2018-06-12 | 合肥迈可罗生物工程有限公司 | A kind of method for improving sow reproductive performance |
| CN110982868A (en) * | 2019-11-29 | 2020-04-10 | 贵州中医药大学 | Co-culture method for improving triterpene content of ganoderma lucidum and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103074240B (en) | Yeast for high-activity feed, and application thereof | |
| CN104770575B (en) | A kind of Radix Astragali probiotics and its preparation method and application | |
| CN108531408B (en) | Rhodotorula mucilaginosa new strain and microecological preparation | |
| WO2019080454A1 (en) | Inonotus obliquus and application thereof | |
| CN105166458A (en) | Fermented selenium-rich plant feed additive resistant to pig heat stress | |
| CN103614307A (en) | Solid ocean red yeast preparation as well as preparation method and application thereof | |
| CN105062905A (en) | Saccharomyces cerevisiae strain for solid fermentation and its application | |
| CN101392223A (en) | Breeding method of microbial feed additive strain | |
| CN105613999B (en) | Improve breeder performance ferment and preparation method thereof | |
| CN108056068B (en) | Method for improving reproductive performance of hens | |
| CN114651915B (en) | Method for preparing metazoan for improving immunity and promoting growth and application of metazoan | |
| CN105199978A (en) | Bacillus coagulans preparation for livestock breeding and preparation method thereof | |
| CN100387704C (en) | Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use | |
| CN110250320A (en) | The preparation method of radix pseudostellariae ferment | |
| CN103330083A (en) | Composite feed for improving immunity of herring and preparation method thereof | |
| CN108323619A (en) | A kind of additive and its application for Eriocheir sinensis health care growth promoting | |
| CN108208398B (en) | Application of cordyceps strain fermentation product as feed additive in improving reproductive performance of boars | |
| CN113080323A (en) | Preparation method of cordyceps sinensis fermentation liquor for improving immunity of piglets | |
| CN108142359B (en) | Method for improving reproductive performance of sows | |
| CN107041488B (en) | Special high-selenium feed for daphnia and use method thereof | |
| CN109423451A (en) | A kind of Wine brewing yeast strain KSA01 and application thereof | |
| CN108323618A (en) | Application of the Cordyceps strain fermentation object as feed addictive in improving cock reproductive performance | |
| CN110651891B (en) | Probiotic preparation for improving growth performance, immunity and nutritional quality of turbot | |
| CN108244356B (en) | Application of cordyceps strain fermentation product as feed additive in improving reproductive performance of dairy cow | |
| CN113826778A (en) | Preparation method of composite probiotic for fermented feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |