The pueraria lobata of internal blood sugar concentration and starch absorption be can inhibit for food product and preparation method thereof
Technical field
The present invention relates to a kind of edible starch meal replacing foods and preparation method thereof, relate more specifically to a kind of diabetes
What people was applicable in can inhibit pueraria lobata meal replacement powder food of starch absorptivity and glucose concentration level and preparation method thereof in vivo, belong to
Nutritional health food and blood sugar reducing food technical field.
Background technology
Cereal is energy source the most main, and the heat provided accounts for about the 53% of diet total amount of heat, wherein
Main nutrition composition is starch.Starch is distributed very wide and carbohydrate storage principal mode in nature.Mostly
All contain starch in the number root (or stem tuber) of higher plants, stem (or lignum), bulb (root and seed), fruit.Starch exists
It after human body is digested, is mainly absorbed in the form of glucose, energy is provided for many tissues and organ.Body blood glucose value is necessary
In the range of certain (normal), the vital movement of human cell can be just normally carried out.When blood glucose often exceeds normal value, just
It is susceptible to suffer from diabetes.
In the treatment of diabetes, dietetic treatment is to treat the basis of each patients with type Ⅰ DM, of equal importance with drug therapy, is had
The diet control of effect can reduce and delay the generation of complication.But the control of diet is confined to the control to staple food intake and
Fasting object containing confectionery, many patients cause malnutrition or even generate hungry disease.For diabetic, far from rice
Become unavoidable selection with millet cake such as wheats.Some does not eat staple food, but uncontrolled to fat and protein, leads to blood glucose mistake
It is high.It cannot rationally keep on a diet and eventually lead to blood glucose and cannot control well, cause metabolic disorder and constitution declines.
In dietary structure, selecting the starch of suitable species seems more important.Typically, starch can be divided into and disappear soon
Change three kinds of starch, slow-digestion starch and resistant starch (resistant starch).Wherein, it is violent to easily cause blood glucose for fast digestible starch
Lifting reaction, resistant starch cannot then generate glucose, but eating mouth feel is very poor, and slow-digestion starch is then since it slowly disappears
Change and absorb, so as to be conducive to the balance of blood glucose.But common starch almost becomes fast digestible starch entirely after gelatinization.It is based on
Such reason, the intake of common starch cause serious decline and inconvenience to quality of life in patients with diabetes and Discussing Convenience.Such as
Where the content of slow-digestion starch is improved in food, so that after being taken in by human body, existing satiety, and avoid the violent of blood glucose
Fluctuation, is an important subject and the content in field of food, this crowd excessively high to blood glucose and obese people it is normal
It lives particularly important, this is also one of the object of the invention.
Specifically, slow-digestion starch (SDS), referring to those can completely be digested and assimilated but slow shallow lake in small intestine
Powder (20-120min);Wherein, slow-digestion starch is in the reticular structure of partially crystallizable, has low degree of swelling, and digesting
Hydrolysis rate approximately constant in journey.Slow-digestion starch, which has, slowly to be digested and assimilated, continuous energy release, maintains postprandial blood sugar steady
State improves glucose tolerance, can also reduce Postprandial insulin secretion, improves body to the sensibility of insulin, prevention and treatment
The various special physiological functions with diet chronic diseases.Slow-digestion starch is as a kind of novel functional product, just
It has just taken into account diabetes nutrition and has cured two big demand of function, not only complied with the trend when food substitutes and drug development in science and technology,
The health demand of consumer is also met, this is to rationally utilizing carbohydrate to prevent the relevant metabolic syndrome of insulin resistance
Important in inhibiting occurs.Therefore, numerous researchers are all conceived to the research of slow-digestion starch.
In the source of starch, kudzu-vine root powder is one of very excellent source.Pueraria lobata is that a kind of rare wild is planted
Object is distributed widely in China, is acquired for a long time by people edible.The content of starch of pueraria lobata is up to 20% or so, is a kind of battalion
Support unique, dietotherapeutic health product.In addition, pueraria lobata must rich in the human bodies such as amino acid and iron needed by human, calcium, selenium, zinc
The trace element needed, primary healthcare functional component substance is Puerarin, daidzein-glycoside, methoxy puerarin, big
The isoflavone compounds such as beans glucoside and daidzein.Therefore, kudzuvine root starch is often eaten, there are the work(such as hypoglycemic, reducing blood lipid, blood pressure lowering
Effect, is known as " long-lived powder ".
Presently commercially available kudzuvine root starch is essentially fecula, slow due to it although can also directly reconstitute as food starch
Digestible starch content is relatively low, gelatinization point is high, needs first to be reconciled with cold water, then reconstitute into paste with boiling water when edible, can be only achieved
The characteristic that shape is glittering and translucent, taste lubrication is fine and smooth.This is very inconvenient for open-air conditions, therefore, can be compared with low temperature
It is reconstituted under degree, being suitble to tourism, the kudzu-vine root powder eaten when lying fallow of going on business, there are strong market demands for food product.
Due to the such important role of above-mentioned slow-digestion starch, in the prior art, people are for the system of slow-digestion starch
It is standby also to have carried out a large amount of further investigation, such as:
CN101361536A discloses a kind of side of organic acid fracturing hot acid solution common corn starch production slowly digestible starch
Method.It is that common corn starch is configured to a concentration of 2-30% (w/v) with the organic acid soln of a concentration of 0.1-10% (w/v)
Corn starch milk, after stirring 20-60min, hot acid solution 5-90min is pushed in 100-150 DEG C of temperature condition;Again to cornstarch
Breast centrifuges 2-10min with the speed of 2000-10000rpm, centrifugal sediment is refrigerated retrogradation 12-240h, then at 50-80 DEG C of temperature
Lower forced air drying 10-20h is spent, 80-150 mesh sieve is crossed after mechanical crushing, is i.e. production is obtained containing more than 20% slowly digestible starch
Product.
CN101518306A is disclosed carries out enzyme modification processing using Pullulanase to ative starch, makes the crystallinity of starch
Improve and can controlled slowly digestible starch preparation method.Including raw starch prepare, add in Pullulanase digested,
Enzyme deactivation, cooling, refrigeration, centrifugation, drying, crushing, sieving and etc..This method be a kind of new type functional food raw material-slowly
The enzyme process technology of preparing of digestible starch.
CN101891831A is disclosed forms short chain amylose based on high-power ultrasonic degradation rice starch, through excessively high
Warm lipid reactant and in short-term crystallization form single-screw v-shaped Microcrystalline Starch-lipid complex, have apparent slow digestion characteristics.
Preparation process is:Rice starch breast (500-600W, less than 25 DEG C) under Ultrasonic Conditions handles 25-30min, then add lipid
(rice quality 20-22%) carry out high temperature complex reaction (140-160 DEG C, 20-25min), then using in short-term crystallization (4 DEG C,
12h) technology obtain more than 70% slow-digestion starch content and in high temperature process processing (90-110 DEG C) slow-digestion starch it is residual
Allowance reaches more than 95% product.
The step of CN102258966A discloses a kind of preparation method of microcapsules slow-digestion starch, the method be:It will
Alcohol soluble protein is put in beaker, adds in ethyl alcohol, and stirring in water bath to alcohol soluble protein is completely dissolved, and is added in oleic acid, is stirred evenly, and is added in
Cornstarch continues to stir.Homogeneous 3 times in high pressure homogenizer, centrifugal spray drying obtains product.Prepared microcapsules are slow
Digestible starch product is in powdered, and starch capsules are good, good mouthfeel, and the content of slow-digestion starch is more than 50%.The side
Method can delay the infiltration of water and the gelatinization of starch made to be difficult to so as to slow in starch particle surface plus protectiveness microcapsule membrane
Digestion, meanwhile, the microcapsules on starch surface improve the taste quality of starch, and starch product is allow directly to eat.
CN105017435A disclose a kind of octenyl succinic acid anhydride starch rich in slowly digestible starch preparation and its
Purposes, the preparation method include the following steps:Step 1 weighs starch, adds in water;Step 2,1 products therefrom of whipping step,
Control ph is alkalinity;Step 3 adds in octenyl succinic acid anhydride, stirring, reaction into step 2 products therefrom;Step 4 is adjusted
The pH value of step 3 products therefrom is to neutrality;Step 5,4 products therefrom of centrifugation step, must precipitate;Step 6 carries out the precipitation
It filters, clean, drying to get the high expansion coefficient octenyl succinic acid anhydride oat starch.It is made by the method pungent
Alkenyl succinic anhydride starch, slowly digestible starch content is high and stablizes.Available for preparing microcapsule wall material applied to medicine etc.
In industry, a kind of novel health food is alternatively arranged as, and its product can reduce cholesterol, reduce blood glucose fluctuation, prevent insulin
Parasecretion is the ideal chose of diabetic and people to lose weight.
CN105348397A discloses a kind of method for being chemically combined enzyme process and efficiently preparing thermostable type slow-digestion starch, with wax
Matter corn is raw material, and preparation method includes the following steps:(1) acidolysis is handled;(2) drying is washed;(3) heating pre-gelatinized processing;
(4) amylosucrase is handled;(5) it washs, autoclaving, 4 DEG C of preservations;(6) it is freeze-dried.The method overcomes waxy corn
Starch the defects of viscosity is high under a high concentration condition, enzyme reaction efficiency is low, the slow-digestion starch thermal stability prepared is good, can be with
Supplementary material as food processing carries out heat treatment, therefore product has a vast market prospect again, is a kind of novel
, simple, efficient, safety thermostable type slow-digestion starch preparation method.
Although disclosing a variety of methods for preparing slow-digestion starch in the prior art, for preparing, gelatinization point is low, contains
Measuring the new method of high slow-digestion starch, there are still the necessity for continuing research.
In addition to slow-digestion starch, Starch blockers (also known as kidney bean albumen, English name phaseolin) are also a kind of reduction blood
Sugar important substance, be the natural component extracted from navy bean, without any harmful drug into.It can prevent simultaneously
Improve the obesity caused by absorbing fattiness, it is safe and reliable, it is without any side effects to human body, it is a kind of effectively control carbon
The tool of hydrate heat.The mechanism of action of Starch blockers is to be combined with the alpha-amylase in enteron aisle, makes alpha-amylase
Starch can not be cut off, so that most starch can excrete, do not release with complete molecular conformation by digestive system
What heat let alone.Slimming can be played, decomposition combustion fat, inhibit fat absorption;Prevent adult diseases, such as hypoglycemic, drop blood
Many effects such as fat, blood pressure lowering.
At present, although the food containing slow-digestion starch and Starch blockers can be suitable for diabetes patient, due to sugar-free
Or poor taste caused by low sugar the shortcomings that being always this based food.And stevioside glycosides (the also known as stevia rebaudianum from qualities of stevia extract
Glucoside) sugariness effect be about 210 times of sucrose, there is 0 heat, be preferably to add for diabetic keratopathy and low-calorie diet
Add agent.In addition, stevioside does not participate in the metabolism of human body.It is safe and reliable, nontoxic, without side-effects, it is high sugariness, low in calories
(calorific value is equivalent to the 1/300 of sucrose).Sweet taste is similar to sucrose, but Duration Ratio sucrose is long.To cardiovascular and cerebrovascular diseases, obesity
Disease, diabetic have auxiliary treatment functio, and the carbohydrate substitute of ketosis (PKU) patient can be also urinated as phenylpropyl alcohol.Be after sucrose,
The natural product of replacing sugar that the third has Development volue and healthy functions except beet sugar is described as " third sugar source " in the world.Extensively
Applied in varieties of food items, beverage, medicine, daily chemical industry product.At present, the extraction of domestic and international stevioside is done with STEVIA REBAUDIANA
Leaf is raw material, STEVIA REBAUDIANA fresh leaf through artificial picking leaves, dry or air-dry, stevioside factory is transported after compressed packing and is soaked
Bubble extraction.
As described above, a variety of methods for preparing slow-digestion starch and its food are disclosed in the prior art, but for preparing
The method of higher slow-digestion starch content and being eaten containing the nutritional meal replacement of slow-digestion starch and Starch blockers for improvement mouthfeel
There is still a need for continuing to study, this has very important significance product particularly with diabetic and obesity patient, can be very big
Improve and improve the quality of life of diabetic and obese people in ground.
Invention content
Based on develop the preparation method of new slow-digestion starch, comprising slow-digestion starch, improve nutrition and mouthfeel it is strong
The purpose of health meal replacing food, present inventor has performed a large amount of deeply research and development, are paying a large amount of creative work and process
After further investigation is explored, so as to complete the present invention.
Specifically, the invention mainly relates to following aspects.
The first aspect, the present invention provides a kind of pueraria lobata meal replacement powders for inhibiting blood sugar concentration and starch absorptivity in vivo
Preparation method, the preparation method includes the following steps:
(a) complex nucleus material powder is prepared, including step:(a1) using dry vegetable powder, dry stevia rebaudian leaf powder and selected from mountain
The first core material powder is prepared at least one of medicine, black rice, konjaku, winter wheat, corn, duck wheat, pulp of a pumpkin;(a2) make
The second core material powder is prepared with resistant starch, appropriate flavouring agent and soybean protein isolate;First core is mixed in proportion
Material powder and the second core material powder obtain complex nucleus material powder;
(b) slow-digestion starch is prepared using pueraria starch;
(c) using made from gelatin, pectin, sodium alginate and above-mentioned steps (b) slow-digestion starch prepare in shell material matrix;
(d) microparticle powder is prepared using the complex nucleus material powder and the interior shell material matrix;
(e) shell slurries are prepared:The slurries include at least Starch blockers, lactalbumin powder component;
(f) using the microparticle powder of the shell slurries coating step (d), the pueraria lobata generation meal is prepared through dry
Powder.
Wherein, in the preparation method of pueraria lobata meal replacement powder of the present invention, the step (a) is prepared as follows:
It (a1) will be more than any one in Chinese yam, black rice, winter wheat, corn, duck wheat, konjaku, pulp of a pumpkin
Main material co-grinding is to 50-200 mesh, and vegetable powder, dry stevia rebaudian leaf are crushed to 60-300 mesh, according to mass ratio 10:(1-3):
The ratio of (0.1-0.5) weighs above-mentioned main material powder, vegetable powder, dry stevia rebaudian leaf powder, adds as its gross mass 3-5
Purified water again, pot boil stirring 30-60 minutes, then with high pressure homogenizer homogeneous, concentrate, and spray drying obtains described first
Core material powder;(a2) the second core material powder is prepared using resistant starch, appropriate flavouring agent and soybean protein isolate;In proportion
It mixes the first core material powder and the second core material powder obtains complex nucleus material powder.
In the step (a2), resistant starch, soybean protein isolate and the flavor added in during the second core material powder is prepared
The mass ratio of agent is 10:(1-10):(0.01-0.1).
In the step (a), the mixed proportion of the first core material powder and the second core material powder is 1:(0.2-1).
In the step (a1), the inlet air temperature of the spray drying is 180 DEG C, and leaving air temp is 120 DEG C.
In the step (a1), the Chinese yam, black rice, winter wheat, corn, duck wheat, konjaku, pulp of a pumpkin etc. are day
Chang Gongzhi and the main material that can be bought in the market, preferably fully dry.Wherein described high pressure homogenizer is also in homogeneous field
Commonly used equipment can will treat that homogeneous substance is sufficiently mixed uniform and granularity very little.
Wherein, powder of the Vegetable powder for daily well known vegetables after dehydration and drying, crushing and processing, for example, celery, asparagus lettuce,
It is one or more kinds of in black fungus, fiddlehead, cauliflower, rape, Chinese cabbage, spinach, carrot, lily root, eggplant and cucumber etc.
Composition is capable of providing more rich nutrition and dietary fiber.
Dry stevia rebaudian leaf powder is that the STEVIA REBAUDIANA blade crushing and processing being dried form.
In the step (a), the resistant starch (also referred to as resistant starch) is known in the art, commercially available can be obtained
Take or take general technology known in the art to prepare.
Resistant starch of the present invention includes the native starch rich in resistant starch, such as amylomaize;
Also include modified common starch, such as the aging starch formed after being cooled down by gelatinized starch, the modification of physical or chemical modification
Starch, such as such as acetyl starch, hydroxypropul starch, thermal denaturation starch, phosphorylated starch.The preparation of resistant starch is this
Known general technology in field, prepared resistant starch effect is also substantially close to details are not described herein.
Preferred resistant starch is native starch or aging starch rich in resistant starch in the present invention.
In the preparation method of pueraria lobata meal replacement powder of the present invention, the flavouring agent in the step (a2) is can to improve mouth
The substance of sense, selected from least one of sweetener, fumet.The sweetener is selected from qualities of stevia extract or stevioside,
The fumet is selected from maltol, ethylmaltol.
Wherein, qualities of stevia extract refer to mainly include stevioside composite extract, can be purchased from the market or
It is prepared using the conventional method in state of the art, details are not described herein.
Stevioside is commonly called as stevioside (Stevioside), is the novel day that essence carries from catananche's STEVIA REBAUDIANA
Right sweetener has zero calory characteristic.Stevioside is a kind of by the different numbers of this tetracyclic diterpene compound connection of steviol
Glycocide composition glucoside mixture.It can be commercially available by market or be obtained using prior art conventional method.
Wherein, the stevioside in the present invention is selected from Stevioside (stevioside) (St), steviolbioside
(steviolbioside);Content rebaudioside-A (rebaudioside A) (R-A), rebaudioside B (rebaudioside B) (R-
B), rebaudioside C (rebaudioside C) (R-C), rebaudioside D (rebaudioside D) (R-D), rebaudioside E
It is one or more in (rebaudioside E) (R-E).
In the preparation method of pueraria lobata meal replacement powder of the present invention, when the flavouring agent in the step (a2) is that STEVIA REBAUDIANA carries
When taking object, although trophism is more excellent, mouthfeel will be weaker than pure stevioside.The present invention it was proved that, with 100:1
To 1000:1 (qualities of stevia extract:Maltol) weight ratio when maltol is added in into qualities of stevia extract, can be in oral cavity
In sweet taste be slowly presented and increase persistence, so as to improve sugariness and persistence.
In the preparation method of pueraria lobata meal replacement powder of the present invention, soybean protein isolate is using low temperature desolventizing Soybean Meal as original
Expect the full price albumen of production, for protein content more than 90%, amino acid classes there are nearly 20 kinds, and contain amino needed by human
Acid is very excellent plant-derived albumen low in calories, and can replace meat proteins.It is usually commercially available in this field to acquire.
In the preparation method of meal replacement powder of the present invention, the step (b) specifically comprises the following steps:
(b1):At room temperature, pueraria starch is added in purified water, it is 10-20%'s to be configured to weight percent concentration
Then starch emulsion adds in malic acid or aqueous citric acid solution that molar concentration is 0.5mol/L, is warming up to 40-50 DEG C and stirs
It 20-30 minutes, is then centrifuged for, collects precipitation solid, and be dried in vacuo and obtain preliminary acidified starch;By above-mentioned preliminary acidified starch
The starch emulsion for being configured to that weight percent concentration is 5-15% in purified water is added to, the acetic acid for then adding in 1mol/L is water-soluble
Liquid stands acidolysis 30min, then is handled 3-5 minutes under power 300-400W with microwave, is then centrifuged for collecting precipitation solid, purifying
Water fully washs, and is dried in vacuo completely, obtains acidified starch;
(b2):The acidified starch is configured to the starch emulsion that weight percent concentration is 10-40% with purified water, is filled
Divide stirring and dissolving complete, it is 6-7.5 to add in food-grade sodium carbonate or sodium bicarbonate tune pH, then the boiling 10-60 in autoclave
Minute, then cooled to room temperature, obtains gelatinized starch lotion;
(b3):The beta amylase and glucose that dosage is 100~500U/g starch are added in into the gelatinized starch lotion
Turn glycosides enzyme dual-enzyme system, the pH value of regulation system is 6-7, then oscillation enzymolysis 1-3 hours at 45-60 DEG C;Boiling water bath makes enzyme
Inactivation adds in the Pullulanase that dosage is 50~500U/g starch, and tune pH value is 5-7, then the oscillation enzymolysis 3- at 50-65 DEG C
5 hours, after high temperature enzyme deactivation, and cooled to room temperature obtains enzymolysis product;It is high by the enzymolysis product natural cooling
Speed centrifugation, collects precipitation, is fully washed with purified water, is placed in air dry oven and dries 12 hours, crushed 200-300 mesh sieve,
Up to slow-digestion starch.
Wherein, in the step (b1), the ratio of pueraria starch and the acid in terms of mole (mol) in terms of quality gram (g)
Example is 100 (g):0.3-1(mol).
It is combined with double enzymes of beta amylase the inventors discovered that the glucose turns glycosides enzyme and can play optimal preliminary enzyme
Cut efficiency, and with Pullulanase be further combined can not only thorough enzymatic starch, and the disconnected of starch long-chain can be influenced
Degree and the content of multi-branched starch molecule are split, the final content and high-temperature stability for improving slow-digestion starch.
In the preparation method of pueraria lobata meal replacement powder of the present invention, the step (c) is specific as follows:Step (b) is made
Slow-digestion starch, gelatin, pectin and sodium alginate, according to 1:(0.3-0.5)(0.2-0.3):0.1 mass ratio is added to pure
Change in water (purifying water quality is 1-2 times of above-mentioned material gross mass), and be sufficiently stirred, be uniformly mixed it, obtain interior shell material
Matrix.
In the preparation method of pueraria lobata meal replacement powder of the present invention, the step (d) is specific as follows:By the complex nucleus material
Powder is added in the interior shell material matrix of step (c) preparation, adds the purified water of 3-5 times of gross mass, is sufficiently stirred
Even, spray drying obtains microparticle powder.
In the step (d), the mass ratio of the complex nucleus material powder and the interior shell material matrix is 1:0.5-1.5,
It may be, for example, 1:0.5、1:1 or 1:1.5.
In the step (d), the temperature of the spray drying is adjusted to that epigranular can be obtained, outer layer uniformly coats
Particle subject to, formed core-inner casing configuration microparticle powder.
In the preparation method of pueraria lobata meal replacement powder of the present invention, step (e)-(f) is specific as follows:Starch is blocked
Agent and PURE WHEY are added in the hydroxypropyl cellulose aqueous solution that mass percent concentration is 5-10%, are sufficiently stirred, are obtained
To shell slurries spraying;The shell slurries are uniformly sprayed onto to microparticle prepared by the step (d) of rolling stirring again
Powder surface, persistently stirs evenly after having sprayed, and is then dried in vacuo to get the pueraria lobata meal replacement powder.
In the step (e), the Starch blockers, PURE WHEY, hydroxypropyl cellulose aqueous solution mass ratio
It is 1:(2-3):(5-10), may be, for example, 1:2:10.Wherein, the mass ratio of the microparticle powder and shell slurries is 1:0.5-1.
The second aspect, the present invention relates to pass through pueraria lobata meal replacement powder made from above-mentioned preparation method.
In terms of third, the present invention relates to the meal replacing foods for including above-mentioned pueraria lobata meal replacement powder, such as biscuit.
Of the present invention comprising in the meal replacing food of pueraria lobata meal replacement powder, in addition to Starches are different, the food
The production method of product is identical with using the normal food of starch.The meal replacing food includes biscuit, steamed bun, cake etc., preferably cake
It is dry.
Of the present invention comprising in the meal replacing food of pueraria lobata meal replacement powder, the food is biscuit.Its production method packet
It includes:The pueraria lobata meal replacement powder of the 20-60wt% present invention is added in flour as flour material, remaining operation makes with common biscuit
It is identical.For example, adding suitable quantity of water in flour, pH is adjusted, dough mixing machine is placed in and carries out knead dough and dough standing tabletting is made, using ability
Domain bakes the conventional method for making biscuit, and pueraria lobata is made through a series of processes such as overbaking and is done for refreshment.
The comprehensive collaboration effect that the present invention passes through the Multiple factors such as specific preparation method, the selection of specific component and additive
Fruit, meal replacement powder of the present invention have good blood glucose level control, reducing blood lipid, inhibit starch absorption, satiety strong and good
A variety of excellent properties such as mouthfeel, so as to be carried for various people especially hyperglycemia population, diabetic and obese people etc.
The healthy food of safe nutrition has been supplied, has been had a good application prospect and industrial production potential.
In addition, present invention original creation core material-inner casing-shell beads structure, after eating, gastroenteric environment is enabled in shell
Starch blockers are combined at the first time with the amylase in gastrointestinal tract, inhibit enzymolysis of the amylase to starch, so that big portion
Point starch is excreted by digestive system, is not absorbed by the body, can play steady blood glucose, slimming effect, meanwhile, generation
The Puerarin that meal powder includes is even more to have the excellent effects such as hypoglycemic, reducing blood lipid, blood pressure lowering.
Specific embodiment
Below by specific preparation example and embodiment, the present invention is described in detail, but these exemplary embodiments
Purposes and purpose be only used for enumerating the present invention, any type of any limit not is formed to the real protection scope of the present invention
It is fixed, it is more non-that protection scope of the present invention is confined to this.
Preparation example 1:Slow-digestion starch is prepared by pueraria starch
(1):At room temperature, pueraria starch is added in purified water, is configured to the shallow lake that weight percent concentration is 10-20%
Powder lotion, then add in malic acid that molar concentration is 0.5mol/L or aqueous citric acid solution (pueraria starch in gram with
Mole meter acid ratio be 100:0.5) 50 DEG C, are warming up to and is stirred 30 minutes, is then centrifuged for, collects precipitation solid, and true
Sky is dried to obtain preliminary acidified starch;Above-mentioned preliminary acidified starch is added in purified water and is configured to weight percent concentration and is
15% starch emulsion, then add in 1mol/L aqueous acetic acid stand acidolysis 30min, with microwave under power 300W
Reason 3 minutes is then centrifuged for collecting precipitation solid, and purified water is fully washed, and is dried in vacuo completely, obtains acidified starch;
(2):The acidified starch is configured to the starch emulsion that weight percent concentration is 30% with purified water, is fully stirred
It is complete to mix dissolving, it is 6.5 to add in food-grade sodium carbonate or sodium bicarbonate tune pH, then boiling 40 minutes in autoclave, then
Cooled to room temperature obtains gelatinized starch lotion;
(3):Beta amylase and the Portugal that dosage is respectively 200U/g, 300U/g starch are added in into the gelatinized starch lotion
Grape sugar turns glycosides enzyme, and the pH value of regulation system is 6.5, then oscillation enzymolysis 3 hours at 55 DEG C;Boiling water bath inactivates enzyme, adds in
Dosage is the Pullulanase of 400U/g starch, and it is 7 ± 0.3 to adjust pH value, then oscillation enzymolysis 5 hours at 60 DEG C, after it is high
Warm enzyme deactivation, and cooled to room temperature, obtain enzymolysis product;By the enzymolysis product natural cooling, high speed centrifugation, it is heavy to collect
It forms sediment, is fully washed with purified water, be placed in air dry oven and dry 12 hours, crushed 200 mesh sieve to get slow-digestion starch.
Slow-digestion starch content is measured using Englyst methods well known in the art, measuring above-mentioned slow-digestion starch content is
65.3%.
Comparative example 1:
(1):At room temperature, pueraria starch is added in purified water, is configured to the starch that weight percent concentration is 10%
Then lotion adds in the malic acid (ratio of pueraria starch in gram and acid in mol that molar concentration is 0.5mol/L
It is 100:0.5) 50 DEG C, are warming up to and is stirred 30 minutes, is then centrifuged for, collects precipitation solid, and be dried in vacuo and obtain preliminary acid
Solve starch;Above-mentioned preliminary acidified starch is added to the starch emulsion for being configured to that weight percent concentration is 15% in purified water,
Then the aqueous acetic acid for adding in 1mol/L stands acidolysis 60min, is then centrifuged for collecting precipitation solid, and purified water is fully washed,
And be dried in vacuo completely, obtain acidified starch;
(2):The acidified starch is configured to the starch emulsion that weight percent concentration is 30% with purified water, is fully stirred
It is complete to mix dissolving, it is 6.5 to add in food-grade sodium carbonate or sodium bicarbonate tune pH, then boiling 40 minutes in autoclave, then
Cooled to room temperature obtains gelatinized starch lotion;
(3):The beta amylase that dosage is 200U/g starch, the pH value of regulation system are added in into the gelatinized starch lotion
It is 6.5, then oscillation digests 3 hours at 55 DEG C;Boiling water bath inactivates enzyme, adds in the Propiram that dosage is 400U/g starch
Enzyme, it is 7 ± 0.3 to adjust pH value, then oscillation enzymolysis 5 hours at 60 DEG C, after high temperature enzyme deactivation, and cooled to room temperature,
Obtain enzymolysis product;By the enzymolysis product natural cooling, high speed centrifugation collects precipitation, is fully washed with purified water, be placed in drum
Wind drying box dry, pulverize 200 mesh sieve to get slow-digestion starch.
Slow-digestion starch content is measured using Englyst methods well known in the art, measuring above-mentioned slow-digestion starch content is
52.3%.
It can be seen that in the single enzyme of preliminary acidolysis and under conditions of lacking microwave treatment, slow-digestion starch content is shown
It writes and declines.
It is noted that further single factor test contrast experiment proves, turn glycosides enzyme even if only lacking glucose or only lack
One factor of few microwave treatment, the slow-digestion starch content obtained are still respectively present 5%, 6% or so drop-out value.This card
Bright microwave treatment and complex enzyme hydrolysis not only promote reaction to carry out, and also there is synergistic effect therebetween.
In addition, the inventors discovered that, when used beta amylase concentration turns glycosides enzyme less than glucose, it is prepared
Slow-digestion starch heat-resistant stability effect is more excellent, otherwise poor, this may be from the starch chain length and branching rate function of different digestions
It is different related.It is demonstrated experimentally that beta amylase turns the ratio between glycosides enzyme concentration close to 2 with glucose in the present invention:It is optimal when 3, it steams
It is only 10.7% (content of slow-digestion starch reduces percentage after boiling) to boil the heat loss after 30min.
Wherein, the measuring method of stability of the present invention is:The slow-digestion starch product of preparation is taken, first surveys slow digestion therein
Content of starch, then add it to and a certain concentration is made into purified water, stirring is boiled 60 minutes, then filters, is dry, surveying again
The content of slow-digestion starch therein is measured, is compared with original content, so as to investigate the reduction of the content of its slow-digestion starch
Percentage, specific formula for calculation are as follows:Content reduces (%)=(content after content-heat treatment before heat treatment)/(heat treatment
Preceding content) × 100%.
Embodiment 2:The preparation of pueraria lobata meal replacement powder
(1) by Chinese yam, black rice, corn, duck wheat, konjaku, pulp of a pumpkin according to 1:1:1.5:1:2:2 ratio mixing weighs
500g is crushed to 50 mesh or so as main material, and dehydrated vegetables (asparagus lettuce, black fungus, fiddlehead, Chinese cabbage, the spinach of weight ratios such as includes
Dish, carrot), dry stevia rebaudian leaf be crushed to 100 mesh, according to mass ratio 10:2:0.5 ratio is to above-mentioned main material powder, Vegetable powder
Last, dry stevia rebaudian leaf powder is weighed, and adds the purified water for 5 times of its gross mass, pot boils stirring 60 minutes, then equal with high pressure
Matter machine homogeneous concentrates, and spray drying obtains the first core material powder.
In mass ratio 10:1:0.1 ratio (adds amylomaize, soybean protein isolate, qualities of stevia extract
Have the maltol of 51 percent weight ratios) be uniformly mixed obtain the second core material powder, and therefrom weigh 200g and above-mentioned first
Core material powder is uniformly mixed to obtain complex nucleus material powder.
(2) slow-digestion starch, gelatin, pectin and the sodium alginate that above-mentioned preparation example 1 is prepared, according to 1:0.3:
0.2:0.1 mass ratio is added in purified water (purifying water quality is 2 times of above-mentioned material gross mass), is sufficiently stirred, mixes
Uniformly, interior shell material matrix is obtained.
(3) according to mass ratio 1:Above-mentioned complex nucleus material powder is added to the interior shell material of step (2) preparation by 1 ratio
In matrix, the purified water of the complex nucleus material powder and interior 4 times of shell material matrix gross mass is added, is stirred, and spray
Mist is dried, and obtains microparticle powder.
(4) Starch blockers and PURE WHEY are added to the hydroxypropyl cellulose water that mass percent concentration is 10%
In solution (Starch blockers, PURE WHEY, hydroxypropyl cellulose aqueous solution mass ratio be 1:2:10) it, is sufficiently stirred, obtains
To shell slurries spraying;Shell slurries are uniformly sprayed onto to microparticle powder table prepared by the step (3) of rolling stirring again
Face (shell slurries dosage is 0.5 times of quality of microparticle powder), persistently stirs evenly after having sprayed, is then dried in vacuo to get institute
State pueraria lobata meal replacement powder.
Embodiment 3:The preparation of pueraria lobata meal replacement powder
By winter wheat, black rice, corn, duck wheat, konjaku, pulp of a pumpkin according to 1:1:1.5:1:2:2 ratio mixing weighs
400g is crushed to 60 mesh as main material, dehydrated vegetables (asparagus lettuces of weight ratios, black fungus, fiddlehead, spinach, the carrot such as including),
Dry stevia rebaudian leaf is crushed to 80 mesh, according to mass ratio 10:3:0.2 ratio is to above-mentioned main material powder, vegetable powder, dry stevia rebaudian leaf powder
It is weighed, adds the purified water for 4 times of its gross mass, pot boils stirring 40 minutes, dense then with high pressure homogenizer homogeneous
Contracting, spray drying, obtains the first core material powder.
In mass ratio 10:3:0.03:0.05 ratio is by aging resistant starch, soybean protein isolate, Stevioside, maltol
It is uniformly mixed and obtains the second core material powder, and therefrom weigh 150g and be uniformly mixed to obtain complex nucleus material with above-mentioned first core material powder
Powder.
Slow-digestion starch, gelatin, pectin and the sodium alginate that above-mentioned preparation example 1 is prepared, according to 1:0.3:0.3:
0.1 mass ratio is added in purified water (purifying water quality is 2 times of above-mentioned material gross mass), is sufficiently stirred, is uniformly mixed,
Obtain interior shell material matrix.
According to mass ratio 1:Above-mentioned complex nucleus material powder is added to the interior shell material of step (2) preparation by 0.8 ratio
In matrix, the purified water of the complex nucleus material powder and interior 5 times of shell material matrix gross mass is added, is stirred, and spray
Mist is dried, and obtains microparticle powder.
Starch blockers and PURE WHEY are added to the hydroxypropyl cellulose aqueous solution that mass percent concentration is 5%
In (wherein Starch blockers, PURE WHEY, hydroxypropyl cellulose aqueous solution mass ratio be 1:2:10) it, is sufficiently stirred, obtains
To shell slurries spraying;Shell slurries are uniformly sprayed onto to microparticle powder table prepared by the step (3) of rolling stirring again
Face (shell slurries dosage is 0.8 times of quality of microparticle powder), persistently stirs evenly after having sprayed, is then dried in vacuo to get institute
State the pueraria lobata meal replacement powder for inhibiting blood sugar and starch absorptivity in vivo.
Embodiment 4:Pueraria lobata meal replacement powder inhibits blood sugar concentration test
Blood sugar concentration, grouping specific as follows are tested using CD-1 male mices as experimental animal (20~30g of weight):
Test A groups:To meal replacement powder made from 10 mouse difference gavage embodiments 2 (feeding amount for 200mg meal replacement powders/
100g mouse weights).
Test B groups:To made from 10 mouse difference gavage embodiments 3, (feeding amount is 200mg/100g mouse to meal replacement powder
Weight)..
Blank group:Distinguish gavage purified water to 10 mouse.
Control group:To 10 mouse difference gavage pueraria starches (untreated raw starch, feeding amount standard in the present invention
Ibid).
Wherein, all blood glucose test results of above-mentioned every group of mouse are averaged, fasting 8 hours before feeding, are measured
The blood glucose initial value of each group, and the time segment after feeding takes blood to measure blood glucose value with blood glucose meter tail vein respectively.As a result see
Following table.
It can be seen that the pueraria lobata meal replacement powder of the present invention can inhibit the release and absorption of starch, can well inhibit simultaneously
Smooth blood sugar level, it is therefore prevented that the drastically raising of glycemic peaks after ingesting due to the presence of STEVIA REBAUDIANA and Puerarin, has
Further hypoglycemic effect, intake is close to fasting level after three hours, available for diabetic and obesity crowd
Daily supplement diet or for food and drink eat.
In addition, pueraria lobata meal replacement powder of the present invention is made an addition to containing cream, flour and egg etc. the invention further relates to a kind of
In food material, can also baked (100-180 DEG C) process or hot-working food, such as steamed bun, biscuit, cake etc. is made.It is high
The pueraria lobata meal replacement powder of the present invention processed under the conditions of temperature still keeps the activity of certain inhibition blood glucose, can effectively reduce baking food
The Starch Hydrolysis and absorptivity of product can not only meet mouthfeel but also not increase blood glucose burden.
Embodiment 5:Biscuit containing pueraria lobata meal replacement powder
The pueraria lobata meal replacement powder that 60wt% embodiments 2 or 3 obtain is added in 500g flour, adds suitable quantity of water, pH 6.8 is adjusted, puts
It carries out knead dough in dough mixing machine and dough is made to stand tabletting, the conventional method for making biscuit is baked using this field, through overbaking
Pueraria lobata is made etc. a series of processes to do for refreshment.
Embodiment 6:Food containing pueraria lobata meal replacement powder inhibits blood sugar concentration test
Test method:Healthy population is chosen, is divided into two groups, one group of 10 people, men and women is fifty-fifty;After fasting 12 hours, eat respectively
100 grams are done for refreshment, 1 as a child with Roche blood glucose meter to measure everyone blood glucose respectively afterwards dense with common biscuit and the pueraria lobata of the present invention
Degree variation percentage, every group is averaged.As a result it is as follows:
Common biscuit group:Averagely increase 46.7%;
Pueraria lobata does group for refreshment:Averagely increase 11.7%.
Pass through Experimental Comparison, it was confirmed that product of the present invention has good thermal stability, even if after hot working, still having good
The effect of good inhibition level of postprandial blood sugar.To find out its cause, proportioning and selection in addition to specific components, this is with the present invention by starch
It absorbs blocking agent to carry out being bonded with certain relationship in casing part, sodium alginate and PURE WHEY condense together to form package
Protective film protection starch absorbs blocking agent and is not affected by high temperatures, and can release starch at the first time again after consumption and absorb blocking
Agent is combined rapidly with amylase, and human body is blocked to prevent blood glucose from increasing rapidly the subsequent absorption of starch;In addition, inner core particles contain
The hypoglycemic component such as some Puerarins further persistently inhibits blood sugar concentration within the subsequent period.
In conclusion inhibit the release of starch and absorption, the pueraria lobata generation of balance low levels the present invention provides a kind of
Meal powder and preparation method thereof and the food for being related to meal replacement powder of the present invention.The slow digestion being prepared by ad hoc approach
Starch, with reference to unique other components and unique preparation method, so as to which the meal replacement powder and its system with excellent properties can be obtained
Product, the diet part for being very suitable for hyperglycemia such as diabetic and obese people replaces or supplements, so as to good
Economic value and industrial production potential.
It should be appreciated that the purposes of these embodiments is merely to illustrate the present invention and is not intended to limitation protection model of the invention
It encloses.In addition, it should also be understood that, after reading the technical contents of the present invention, those skilled in the art can make the present invention each
Kind change, modification and/or variation, all these equivalent forms equally fall within the guarantor that the application the appended claims are limited
Within the scope of shield.