CN108112510A - A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy - Google Patents
A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy Download PDFInfo
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- CN108112510A CN108112510A CN201711338377.8A CN201711338377A CN108112510A CN 108112510 A CN108112510 A CN 108112510A CN 201711338377 A CN201711338377 A CN 201711338377A CN 108112510 A CN108112510 A CN 108112510A
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- fish
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 80
- 230000000366 juvenile effect Effects 0.000 title claims abstract description 39
- 230000002180 anti-stress Effects 0.000 title claims abstract description 22
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 16
- 238000000053 physical method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 12
- 239000001301 oxygen Substances 0.000 claims abstract description 12
- 238000011282 treatment Methods 0.000 claims abstract description 10
- 230000005855 radiation Effects 0.000 claims abstract description 7
- 230000035882 stress Effects 0.000 claims description 7
- 230000036542 oxidative stress Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 14
- 102000016938 Catalase Human genes 0.000 abstract description 11
- 108010053835 Catalase Proteins 0.000 abstract description 11
- 210000004185 liver Anatomy 0.000 abstract description 11
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract description 9
- 108010012715 Superoxide dismutase Proteins 0.000 abstract description 9
- 241000894007 species Species 0.000 abstract description 5
- 239000003963 antioxidant agent Substances 0.000 abstract description 4
- 230000003078 antioxidant effect Effects 0.000 abstract description 4
- 230000004792 oxidative damage Effects 0.000 abstract description 4
- 238000009344 polyculture Methods 0.000 abstract description 2
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 210000001672 ovary Anatomy 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 241000283070 Equus zebra Species 0.000 description 3
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 3
- 230000003444 anaesthetic effect Effects 0.000 description 3
- 229910052793 cadmium Inorganic materials 0.000 description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 description 3
- 238000005138 cryopreservation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 229910052738 indium Inorganic materials 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000003470 mitochondria Anatomy 0.000 description 3
- 239000008055 phosphate buffer solution Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 102100033220 Xanthine oxidase Human genes 0.000 description 2
- 108010093894 Xanthine oxidase Proteins 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007688 immunotoxicity Effects 0.000 description 2
- 231100000386 immunotoxicity Toxicity 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 229920002120 photoresistant polymer Polymers 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- -1 methyl tertbutyl Chemical group 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- IJRVLVIFMRWJRQ-UHFFFAOYSA-N nitric acid zinc Chemical compound [Zn].O[N+]([O-])=O IJRVLVIFMRWJRQ-UHFFFAOYSA-N 0.000 description 1
- WSGCRAOTEDLMFQ-UHFFFAOYSA-N nonan-5-one Chemical compound CCCCC(=O)CCCC WSGCRAOTEDLMFQ-UHFFFAOYSA-N 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
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- 210000001519 tissue Anatomy 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of physical methods of the enhancing indoor culture juvenile fish anti-stress ability of economy; this method carries out radiation treatment with blue led to juvenile fish; improve the activity of catalase and superoxide dismutase in juvenile fish liver; active oxygen excessive caused by physical stress can effectively be removed; enhance the anti-stress ability of juvenile fish, protect cells from oxidative damage；This method need not add antioxidant and aquifer amendment, avoid the secondary pollution of water body, while also avoid the Species Competition of fish without using Polyculture.
Description
Technical field
The present invention relates to biological technical field, more particularly, to a kind of enhancing indoor culture juvenile fish anti-stress ability of economy
Physical method.
Background technology
Indoor cement pit nursery energy manual control water quality and water temperature completely, make shadow of the fry from water quality and Changes in weather
It rings, therefore Rapid Popularization and extensive use in marine industry.However, due to the excessive intervention of the mankind, facet when cultivating seedling
Facing stress threaten.Stress seedling disease resistance weaken, it is easy to it is dead, be also present with deformity or growth the day after tomorrow even if surviving
Suppression.Therefore, it is in the urgent need to address as aquaculture industry how to improve the anti-stress ability of indoor culture seedling
Problem.The method of traditional enhancing anti-stress ability mainly includes dietary methods（Bait adds antioxidant）, chemical method
（Aquifer amendment）And ecological approach（Sample mixing pattern）.These methods may cause secondary pollution of water and Species Competition, and
And cost is higher.
The content of the invention
It, should it is an object of the invention to provide a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy
Method can significantly increase the anti-stress ability of juvenile fish, and the energy consumption of LED is small, while avoid the water body two caused by conventional method
Secondary pollution and Species Competition.
The technical solution taken to achieve the above object of the present invention is：A kind of enhancing indoor culture juvenile fish of economy resists should
The physical method for the ability of swashing, this method are：By juvenile fish under the irradiation of blue led.It the method increase in juvenile fish liver
The activity of catalase and superoxide dismutase can effectively remove active oxygen excessive caused by physical stress,
Enhance the anti-stress ability of juvenile fish, protect cells from oxidative damage；This method need not add antioxidant and aquifer amendment,
The secondary pollution of water body is avoided, while also avoids the Species Competition of fish without using Polyculture.
Preferably, blue led peak wavelength is 450nm.The condition can not only further alleviate the oxidative stress of juvenile fish,
Also there are the potentiality for promoting juvenile growth development, while enhance the immunity of juvenile fish, alleviate immunotoxicity.
Preferably, irradiation intensity is 0.9 W/m2.Under the conditions of being somebody's turn to do, juvenile fish is quieter, contributes to the people during cultivation
Work operates, and what reduction juvenile fish was subject to stress.
Preferably, the condition of indoor culture juvenile fish is：25-28 °C of water temperature, dissolved oxygen 6.5-8mg/L, pH value 7-8.It should
Under the conditions of, the anaerobic bacteria in water body cannot quickly breed, and reduce the corruption of organic matter, avoid water body nigrescence, fouling phenomenon
Generation, and the self-purification capacity of water body is strong.
Compared with prior art, beneficial effects of the present invention are：A kind of enhancing indoor culture of economy provided by the invention
The physical method of juvenile fish anti-stress ability improves the activity of catalase and superoxide dismutase in juvenile fish liver, can
Active oxygen excessive caused by physical stress is effectively removed, enhances the anti-stress ability of juvenile fish, protects cells from aoxidizing
Damage；This method need not add antioxidant and aquifer amendment, avoid the secondary pollution of water body, while also without using mixed
The pattern of supporting avoids the Species Competition of fish.
Specific embodiment
It is described in further detail with reference to embodiments：
Embodiment 1：A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy, comprises the following steps：AB systems
Zebra juvenile fish takes two of same size, is denoted as I fish and II fish in indoor adaptation 2 weeks, in peak wavelength is 450nm by II fish
Blue led irradiation under handle 4 weeks, then by I fish and II fish exposed to the water body that concentration of cadmium ions is 30 μ g/L（Water temperature
25-28 °C, dissolved oxygen 6.5-8mg/L, pH value 7-8）In, I fish and II fish are taken out after 5 weeks, after fasting for 24 hours, concentration is
0.02% MS-222 anaesthetic treatments carry out liver samples acquisition on ice bag, and the sample of acquisition is immediately placed in cryopreservation tube, use
Liquid nitrogen flash freezer is finally placed in -80 DEG C of refrigerators and saves backup；Phosphate buffer solution (PBS, the pH of liver organization 0.1M
7.4) ice bath is homogenized in glass homogenizer, and homogenate is centrifuged (20min, 5000r/min, 4 DEG C), takes supernatant, is used
Visible spectrophotometer method measures the activity of catalase, and superoxides is measured using xanthine oxidase (hydroxylamine assay)
The activity of mutase, the results are shown in Table 1, as seen from table, compared to I fish, in the II fish liver by blue led radiation treatment
The active higher of catalase and superoxide dismutase；Cadmium exposes the function that can disturb mitochondria, generates more activity
Oxygen, and then oxidative stress is formed, it, can due to the active higher of catalase and superoxide dismutase in II fish liver
The excessive active oxygen of body is effectively removed, so as to improve the anti-stress ability of juvenile fish, protects cells from oxidative damage.
Table 1
| Experimental group | The activity of catalase（Relative level） | The activity of superoxide dismutase（Relative level） |
| I fish | 0.54 | 0.25 |
| II fish | 1.32 | 1.82 |
Embodiment 2：A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy, comprises the following steps：AB systems
Zebra juvenile fish takes two of same size, is denoted as I fish and II fish in indoor adaptation 2 weeks, in peak wavelength is 450nm by II fish
Blue led irradiation under handle 4 weeks, then by I fish and II fish exposed to the water body that concentration of cadmium ions is 30 μ g/L（Water temperature
25-28 °C, dissolved oxygen 6.5-8mg/L, pH value 7-8）In, I fish and II fish are taken out after 5 weeks, after fasting for 24 hours, concentration is
0.02% MS-222 anaesthetic treatments carry out ovary sample collection on ice bag, and the sample of acquisition is immediately placed in cryopreservation tube, use
Liquid nitrogen flash freezer is finally placed in -80 DEG C of refrigerators and saves backup；Phosphate buffer solution (PBS, the pH of ovary tissue 0.1M
7.4) ice bath is homogenized in glass homogenizer, and homogenate is centrifuged (20min, 5000r/min, 4 DEG C), takes supernatant, is used
Thiobarbituricacidα- method measures mda content；Content of nitric oxide is measured using nitrate reductase method, the results are shown in Table 2,
As seen from table, compared to I fish, malonaldehyde is lower with nitric oxide production content in II fish ovaries by blue led radiation treatment；
Cadmium exposes the function that can disturb mitochondria, generates more active oxygens, and then forms oxidative stress, and malonaldehyde is lipid peroxy
The index of change has reacted the cell dysfunction of oxidative stress induction, and the content of malonaldehyde is lower in II fish ovaries, illustrates blueness
LED radiation treatments improve the anti-oxidation stress ability of juvenile fish；A signal of interest of the nitric oxide as fish immunity system
Molecule can induce inflammatory reaction and play an important role in fish immunity system injury and Apoptosis, in II fish ovaries
Nitric oxide production content is lower, illustrates that blue led radiation treatment can help juvenile fish preferably to resist exogenous irritant induction
Immunotoxicity.
Table 2
| Experimental group | Mda content nmol/ (mg prot) | Content of nitric oxide μm ol/ (mg prot) |
| I fish | 2.31 | 0.55 |
| II fish | 1.63 | 0.37 |
Embodiment 3：A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy, comprises the following steps：AB systems
Zebra juvenile fish takes two of same size, is denoted as I fish and II fish in indoor adaptation 2 weeks, in peak wavelength is 450nm by II fish
Blue led irradiation under handle 3 weeks, then by I fish and II fish exposed to the water body that concentration of cadmium ions is 30 μ g/L（Water temperature
25-28 °C, dissolved oxygen 6.5-8mg/L, pH value 7-8）In, I fish and II fish are taken out after 5 weeks, after fasting for 24 hours, concentration is
0.02% MS-222 anaesthetic treatments carry out liver samples acquisition on ice bag, and the sample of acquisition is immediately placed in cryopreservation tube, use
Liquid nitrogen flash freezer is finally placed in -80 DEG C of refrigerators and saves backup；Phosphate buffer solution (PBS, the pH of liver organization 0.1M
7.4) ice bath is homogenized in glass homogenizer, and homogenate is centrifuged (20min, 5000r/min, 4 DEG C), takes supernatant, is used
Visible spectrophotometer method measures the activity of catalase, and superoxides is measured using xanthine oxidase (hydroxylamine assay)
The activity of mutase, the results are shown in Table 3, as seen from table, compared to I fish, in the II fish liver by blue led radiation treatment
The active higher of catalase and superoxide dismutase；Cadmium exposes the function that can disturb mitochondria, generates more activity
Oxygen, and then oxidative stress is formed, it, can due to the active higher of catalase and superoxide dismutase in II fish liver
The excessive active oxygen of body is effectively removed, so as to improve the anti-stress ability of juvenile fish, protects cells from oxidative damage；Its
Middle blue LED die external growth has ZnO microns of graphic arrays, and preparation process is：First one layer is sputtered on LED chip surface
ZnO, obtaining surface using mask lithography technique has the LED chip of photoresist micro meter periodic figure, and gained chip is placed in
In the mixed liquor of zinc nitrate and ammonium hydroxide（Nitric acid zinc concentration is 0.3M）, add and account for 0.02 ‰ methyl tertbutyl of zinc nitrate quality
Ketone and 0.016 ‰ certain herbaceous plants with big flowers acetoacetic esters, then react 3h at 70 DEG C, be cooled to room temperature with deionized water rinsing, dried up with nitrogen,
Finally spending glue removal photoresist has the LED chip of ZnO microns of graphic arrays to get external growth.The methyl- tert added in
Butyl ketone, certain herbaceous plants with big flowers acetoacetic ester and zinc nitrate have synergistic effect, make the micron graphic array tight ness rating higher of growth, greatly improve
The fiber waveguide effect of graphic array, the light extraction efficiency of LED is effectively increased, has saved blue led used of the invention
Energy consumption, reduce cost, it is with good economic efficiency.
Table 3
| Experimental group | The activity of catalase（Relative level） | The activity of superoxide dismutase（Relative level） |
| I fish | 0.53 | 0.27 |
| II fish | 1.29 | 1.78 |
Embodiment of above is merely to illustrate the present invention, and not limitation of the present invention, those of ordinary skill in the art,
In the case of not departing from the spirit and scope of the present invention, it can also make a variety of changes and modification.Therefore, all equivalent technologies
Scheme falls within scope of the invention, and scope of patent protection of the invention should be defined by the claims.
Claims (5)
1. a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy, it is characterised in that：The method is：With
Blue led carries out radiation treatment to juvenile fish.
2. a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy according to claim 1, special
Sign is：Described stress be oxidative stress.
3. a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy according to claim 1, special
Sign is：The blue led peak wavelength is 450nm.
4. a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy according to claim 1, special
Sign is：The irradiation intensity is 0.9 W/m2。
5. a kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy according to claim 1, special
Sign is：The condition of the indoor culture juvenile fish is：25-28 °C of water temperature, dissolved oxygen 6.5-8mg/L, pH value 7-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711338377.8A CN108112510A (en) | 2017-12-14 | 2017-12-14 | A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711338377.8A CN108112510A (en) | 2017-12-14 | 2017-12-14 | A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108112510A true CN108112510A (en) | 2018-06-05 |
Family
ID=62230037
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|---|---|---|---|
| CN201711338377.8A Pending CN108112510A (en) | 2017-12-14 | 2017-12-14 | A kind of physical method of the enhancing indoor culture juvenile fish anti-stress ability of economy |
Country Status (1)
| Country | Link |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102751417A (en) * | 2012-07-24 | 2012-10-24 | 山东大学 | LED (light-emitting diode) tube core with ZnO (zinc oxide)-micron graphic array and preparation method thereof |
-
2017
- 2017-12-14 CN CN201711338377.8A patent/CN108112510A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102751417A (en) * | 2012-07-24 | 2012-10-24 | 山东大学 | LED (light-emitting diode) tube core with ZnO (zinc oxide)-micron graphic array and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| SHUANG-SHUANG YUAN ET AL.: "Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role of blue light emitting diodes", 《ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY》 * |
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