CN108096256B - Cedrelone is preparing the application in anti-Cryptosporidium baileyi drug - Google Patents
Cedrelone is preparing the application in anti-Cryptosporidium baileyi drug Download PDFInfo
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- CN108096256B CN108096256B CN201810082326.1A CN201810082326A CN108096256B CN 108096256 B CN108096256 B CN 108096256B CN 201810082326 A CN201810082326 A CN 201810082326A CN 108096256 B CN108096256 B CN 108096256B
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- cedrelone
- cryptosporidium
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- water
- baileyi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
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- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Abstract
The invention discloses cedrelones and its pharmaceutically acceptable derivates to prepare the application in anti-Cryptosporidium baileyi drug.Cedrelone and its pharmaceutically acceptable derivates and acceptable auxiliary material, are made powder, are added in feed, mix, by daily forage volume feeding chick；Or cedrelone water solube powder is made in cedrelone, and it is soluble in water, by everyday water-drinking amount feeding chick.Being tested by zoogenetic infection confirms that cedrelone can make the metainfective row's egg sac number of Cryptosporidium baileyi reduce 76.6% or more, has preferable anti-Cryptosporidium baileyi effect.
Description
Technical field
The present invention relates to the new opplication of a kind of compound, in particular to cedrelone is preparing anti-Cryptosporidium baileyi drug
In application.
Background technique
Cryptosporidium is subordinate to protozoa (Protozoa), Apicocomplexa (Apicomplexa), sporozoa
(Sporozoasida), Coccidia (Coccidiasina), Eucoccida (Eucoccidiorida), Amy ear suborder
(Eimeriorina), Cryptosporidium section (Cryptosporidiidae), Cryptosporidium (Cryptosporidium), are one
The important enteron aisle parasitic protozoa of kind.It has extensive host range, can infect people, domestic animal, companion animals, wild animal, bird
240 many animals such as class, reptiles, fish cause important Zoonosis Cryptosporidiosis, have significant economic significance and
Public health meaning.Cryptosporidiosis (Cryptosporidiosis) is mainly by fecal oral route through water source, air, food etc.
It propagates, using gastrointestinal mucosa epithelial cell damage diarrhea and Catarrhal respiratory inflammation as typical clinical symptom, causes low age dynamic
The decline of object production performance is dead, can cause chronic, fatal disease for infant and immune deficient patients.
Cryptosporidiosis is poultry and common one of the parasitic disease of wild birds, parasitizes the Cryptosporidium of birds at present
There are 3 to generally acknowledge effective kind: Cryptosporidium baileyi (Cryptosporidium.baileyi), Cryptosporidium meleagridis
(C.meleagridis) and chicken Cryptosporidium (C.galli).Wherein Cryptosporidium baileyi is the sociales for infecting poultry, mainly
The positions such as larynx, tracheae, the bursa of farbricius and the cloaca of poultry are colonized in, causes respiratory tract, Alimentary tract disease, leads to production performance
Decline is even dead.When concomitant immunity inhibition disease or when with other respiratory tracts, alimentary canal cause of disease coinfection, it will cause more
For serious economic loss.In China, fowl Cryptosporidium has report in the area such as Beijing, Guangdong, Sichuan, Shanghai, Henan, Ningxia
Road, chicken, duck, goose, peacock, quail, guinea fowl, silk hair bone chicken can natural infection or artificial challenges.The wherein hidden spore of Guangdong Province duck
Sub- insect infection rate is 85.67~100%, and Beijing duck is 26.5%~64.3%, and Henan Province's pigeon for meat infection rate is 0.98%, chicken sense
Dye rate is 18.8%, and duck infection rate is 16.31%, and quail infection rate is 13.15%, and ostrich infection rate is 1.7%.Due to hidden spore
Sub- worm's ovum capsule volume is small, widely distributed, and conventional chlorination disinfection of drinking water method cannot be killed effectively, therefore defend to the mankind are public
It coins into and seriously threatens.With the expansion of mankind's activity range, the increasing of town and country mobility of people, different types of Cryptosporidium
A possibility that cross-infection, increases, and especially infection probability of the Zoonosis worm kind in crowd will will increase.(the Feng such as Feng
Et al., 2012) positive rate of C.meleagridis is 21.4% in discovery China Shanghai inpatient, and in the city in Shanghai
C.meleagridis, C.baileyi and Eimeria (Eimeriaspp.) egg capsule are detected simultaneously by city's sewage, thus it is speculated that city
Infectious egg capsule in city may be from poultry-farm (Li et al., 2012).Guangdong Province is located in subtropical zone, livestock and poultry cultivation density
Greatly, the processing of sewage, excrement caused by livestock and poultry cultivation lacks cost-effective purification techniques at present, easily causes to external environment
Serious pollution.This seminar also finds that the Cryptosporidium baileyi infection rate of Guangdong pigeon for meat is higher in the investigation of early period.It is hidden
Sporozoite egg capsule has stronger resistance, and traditional disinfection technique is difficult to be killed, and polluted source easily causes between poultry-farm
Cross transmission even breaks out risk.
Currently, there is no ideal active drug for the treatment of this disease.Document report Nitazoxanide, allicin, Ba Long are mould
Extract for treating has certain curative effect.
Cedrelone, the molecular formula of the compound are C26H30O5, molecular weight 422.526, CAS accession number: 1254-85-9.
It is present in one of Meliaceae plant (such as foreign Chinese toon, Chinese toon, toon, the extra large wood of Brazil) root skin chemical component, is a kind of lemon
Acid compounds.Bitter, puckery, cool in nature, root skin decoction is to staphylococcus aureus, pneumococcus, typhoid bacillus and Escherichia coli
Etc. there is inhibiting effect, also there is significant curative effect to gastric and duodenal ulcer, nausea and vomiting, dysentery, hyperlipidemia and scabies etc..
Also studies have reported that cedrelone is inhibiting human cervical carcinoma Hela cell, human breast carcinoma MDA-MB-231 cell Proliferation is adhered to, is moved
Move aspect have the effect of it is certain, with broad-spectrum anti-tumor effect.There has been no cedrelones to have the hidden spore of anti-bayesian so far
The report of sub- worm effect.
Summary of the invention
It is an object of the invention to cedrelones to prepare the application in anti-Cryptosporidium baileyi drug.
The technical solution used in the present invention is:
Cedrelone and its pharmaceutically acceptable derivates are preparing the application in anti-Cryptosporidium baileyi drug.
Cedrelone and its pharmaceutically acceptable derivates are preparing the application in anti-Cryptosporidium baileyi egg capsule drug.
Cedrelone and its pharmaceutically acceptable derivates are preparing the application in feed addictive.
A kind of drug of anti-Cryptosporidium baileyi contains cedrelone or its pharmaceutically acceptable derivative in the drug
Object.
The beneficial effects of the present invention are: powder is made by by cedrelone and acceptable auxiliary material, it is added to feed
In, it mixes, by daily forage volume feeding chick；Or cedrelone water solube powder is made in cedrelone, it is soluble in water, per diem
Normal amount of drinking water feeding chick.Zoogenetic infection experiment confirms that addition cedrelone has the function of preferable anti-Cryptosporidium.Meanwhile
Cedrelone is easily prepared, and extraction cost is relatively low, is easy to develop the anti-Cryptosporidium baileyi drug or feed for low cost
Additive.
Specific embodiment
This explanation is further described below with reference to specific embodiment, but is not so limited.
Embodiment 1
The preparation of cedrelone powder: 4.8g cedrelone (content is not less than 98%) and 95.2g starch is taken to mix, i.e.,
It can.Cedrelone is purchased from Shanghai Yuan Ye Bioisystech Co., Ltd, article No. B30410.
The usage and dosage of powder: above-mentioned 100g powder is admixed in 100kg feed, per diem often feeding amount feeding.
Embodiment 2
The preparation of cedrelone aqueous solvent: 2.7 cedrelones (content is not less than 95%) and 98g glucose are taken to mix, i.e.,
It can.
The usage and dosage of water solube powder: above-mentioned 100g pulvis is dissolved in 100 liters of water, is cut off the water supply before medication to test chicken
2 hours, then press normal water amount feeding.
It is tested below with reference to the anti-Cryptosporidium baileyi of specific description of test cedrelone.
Experimental material:
Control drug: it is reported in the literature with anti-Cryptosporidium effect paromomycin (98%, Shanghai source leaf biotechnology
Co., Ltd, article No. B25850), usage amount is 5mg/kg weight.
Cedrelone: the cedrelone powder of embodiment 1, the cedrelone water solube powder of embodiment 2.
Cryptosporidium baileyi egg capsule: Cryptosporidium baileyi (C.baileyi) Guangdong Strain egg capsule, by Guangdong Academy of Agricultural Sciences
Animal health research institute saves, and using the preceding rejuvenation in SPF chicken body, (SPF chicken is purchased from Guangdong great Hua Nong birds, beasts and eggs Co., Ltd, product
Kind: BWEL-SPF chicken).
Chick: Lingnan Yellow chick, 1 age in days, male and female dual-purpose are provided by Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences, raising
In in the dedicated animal house sterilized；Chicken coop and the equal strict sterilization of vessel used, free choice feeding and drinking pure water.It is seen before test
Chick is examined whether there is or not clinical symptoms and continuous 3d to check excrement whether there is or not Cryptosporidium parvum oocysts suspendeds, it is spare.
Feed: material of brooding is customized by Guangdong Province Xin Nan all feed corporation,Ltds, is free of any anti-Cryptosporidium drug.
Experimental method:
Grouping: by test grouping and being handled 150 Lingnan Yellow breeding chicklings to 3 ages in days, by only weighing, eliminate it is weak young and
Remaining chick is randomly divided into 5 groups by the excessive person of weight, and every group 30, and appropriate adjustment keeps every group of chick total weight roughly equal.
Processing: in addition to I (blank control) group, equal administered by oral gavage Cryptosporidium baileyi egg capsule l × 10 of remaining each group chick6
A/plumage；In addition to Group I and the IIth (positive control) are organized, remaining each group chick feeds or drinks immediately after infection hidden containing resisting
The cedrelone of the feed or water of sporozoite drug, the IIIth group of feed of the feeding containing paromomycin, the IVth group of feeding embodiment 1 dissipates
Cedrelone spice made of agent and feed, the Vth group is drunk made of the cedrelone water solube powder and drinking-water of embodiment 2
The dosage of cedrelone drinking-water, each group drinking-water and feed is all the same.The grouping situation of test chick is shown in Table 1.
Table 1 tests chick grouping
Mental status, feed intake, the excrement situation etc. of chicken group are observed and recorded daily；It weighs to dead chick, dissect；Most
The weight gain and the price of deed of each group chick are calculated afterwards.
Drug effect determination method is mainly according to egg capsule slip after infection；Following to carry out: the 5th day after infection starts daily collect
Excrement, continuous monitoring 15 days.Excrement is taken to eluriate daily, respectively through 40 mesh, 80 mesh, 100 mesh screens, filtrate is through 3000r/min
It is centrifuged 10min, resets and add 15mL saturation sucrose solution in abandoning, shakes up, 1200/min is centrifuged 10min；It is careful immediately after centrifugation to draw
In on blood cell counting plate, (10 × 40 times) observation egg capsules of microscope simultaneously count supernatant 10uL.
Experimental result:
Clinicing symptom observation:
It is inoculated with control group the 5th day of Cryptosporidium baileyi egg capsule, chick appearance spirit is depressed, and coat is slightly random, abdomen from the 6th day
It is obvious to rush down symptom, during which has no chick death.
Average every chick row egg sac number, egg capsule slip and the average weight for calculating separately each group, the results are shown in Table 2 institutes
Show.As shown in Table 2, the III, the IV, V group of average every chick row egg sac number is below the IIth group, and average weight is above the
II group；IV, the V group of average every chick row egg sac number is significantly lower than the IIIth group, and average weight is higher than Group III.Therefore it tests
Confirm that cedrelone can make the metainfective egg sac number of Cryptosporidium baileyi reduce 76.6% or more, the price of deed increases, drink
Water group effect is better than feed addition group, has preferable anti-Cryptosporidium baileyi effect, and be substantially better than paromomycin.
The anti-Cryptosporidium baileyi effect of 2 each group of table
Claims (2)
1. cedrelone is preparing the application in anti-Cryptosporidium baileyi drug.
2. cedrelone is preparing the application in anti-Cryptosporidium baileyi egg capsule drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810082326.1A CN108096256B (en) | 2018-01-29 | 2018-01-29 | Cedrelone is preparing the application in anti-Cryptosporidium baileyi drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810082326.1A CN108096256B (en) | 2018-01-29 | 2018-01-29 | Cedrelone is preparing the application in anti-Cryptosporidium baileyi drug |
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| Publication Number | Publication Date |
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| CN108096256A CN108096256A (en) | 2018-06-01 |
| CN108096256B true CN108096256B (en) | 2019-04-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810082326.1A Active CN108096256B (en) | 2018-01-29 | 2018-01-29 | Cedrelone is preparing the application in anti-Cryptosporidium baileyi drug |
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Effective date of registration: 20210317 Address after: 526300 liujingzui village, hongtaiyang village committee, Nanjie Town, Guangning County, Zhaoqing City, Guangdong Province (500m opposite the village committee) Patentee after: Guangdong xinlinghang Ecological Agriculture Development Co.,Ltd. Address before: 510640 No.21 baishigang street, Wushan, Tianhe District, Guangzhou City, Guangdong Province Patentee before: INSTITUTE OF ANIMAL HEALTH, GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES |
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