CN104688729B - Kainic acid application in preparing anti-Cryptosporidium baileyi medicine - Google Patents
Kainic acid application in preparing anti-Cryptosporidium baileyi medicine Download PDFInfo
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- CN104688729B CN104688729B CN201510057560.5A CN201510057560A CN104688729B CN 104688729 B CN104688729 B CN 104688729B CN 201510057560 A CN201510057560 A CN 201510057560A CN 104688729 B CN104688729 B CN 104688729B
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- kainic acid
- cryptosporidium
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- baileyi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses kainic acid application in preparing anti-Cryptosporidium baileyi medicine.Inventor, by by kainic acid and acceptable adjuvant, making powder, adds to feedstuff, mixing, and by daily forage volume feeding Lingnan Yellow chicken, addition is interpolation 4g kainic acid in every 100kg feedstuff；Or kainic acid is made water solube powder, soluble in water by everyday water-drinking amount feeding white mice, addition is to be dissolved in 2g kainic acid in every 100kg water.Confirm that adding kainic acid can make Cryptosporidium baileyi ovulation capsule amount reduce by more than 78%, has preferable anti-Cryptosporidium effect by zoogenetic infection experiment.
Description
Technical field
The present invention relates to the new opplication of a kind of little molecule, prepare anti-Cryptosporidium baileyi medicine particularly to kainic acid
In application.
Background technology
Cryptosporidiosis is one of poultry and the common parasitic disease of wild birds, parasitizes the Cryptosporidium of birds at present
Have 3 to generally acknowledge effectively to plant: Cryptosporidium baileyi (Cryptosporidium. baileyi), Cryptosporidium meleagridis (C. meleagridis) and chicken Cryptosporidium (C. galli).Wherein Cryptosporidium baileyi is the sociales infecting poultry, mainly posts
The positions such as raw larynx, trachea, fabricius bursa and cloaca poultry, cause respiratory tract, Alimentary tract disease, cause under production performance
Fall is even dead.During when concomitant immunity inhibition disease or with other respiratory tract, digestive tract cause of disease coinfection, it will cause more
Serious economic loss.
In China, fowl Cryptosporidium in Beijing, Guangdong, Sichuan, Shanghai, Henan, the area such as Ningxia all have been reported that, chicken, duck,
Goose, peafowl, Carnis Coturnicis japonicae, guinea fowl, silk hair bone chicken all can natural infection or artificial challenge.Wherein Guangdong Province duck Cryptosporidium spp rate
Being 85.67～100%, Beijing duck is 26.5%～64.3%, and Henan Province's meat pigeon infection rate is 0.98%, and chicken infection rate is 18.8%, duck
Infection rate is 16.31%, and Carnis Coturnicis japonicae infection rate is 13.15%, and Ostriches infection rate is 1.7%.Guangdong Province is located in subtropical zone, livestock and poultry cultivation
Density is big, and sewage, the process of feces produced by current livestock and poultry cultivation lack cost-effective purification techniques, the most to external world environment
Cause severe contamination.Cryptosporidium parvum oocysts suspended has stronger resistance, and traditional disinfection technique is difficult to be killed, contaminant water source electrode
Easily cause cross transmission between poultry-farm and even break out risk.
Treatment currently for primary disease there is no preferable active drug.Document report garlicin, paromomycin treatment, have
Certain curative effect.
Kainic acid, (chemistry is entitled: 2-methylol-3-isopropenyl proline, 2-Carboxy-for Kainic Acid
3-carboxymethyl-4-Isopropenylpyrrolidine), the molecular formula of this compound is C10H15NO4, molecular weight
213.23, CAS accession number: 487-79-6, its chemical constitution is。
Kainic acid is a kind of material extracted from Japanese red Sargassum, is the structure of excitatory amino acid-glutamic acid
Analog, can be combined by the Fructus Jujubae propylhomoserin receptor on granule cell axons tip in hippocampal dentate, increases prominent tip film
Permeability, makes the Excitatory Neurotransmitter in granular cell discharge in a large number, heavily absorbs minimizing.Neurocyte is had relatively by it
High selective injury effect, extensively applies as a kind of instrument medicine in recent years in neurobiology field.
Kainic acid is the most not yet had to have the report of anti-Cryptosporidium baileyi effect, known to kainic acid
Character cannot infer whether it has the effect of anti-Cryptosporidium baileyi.
Summary of the invention
It is an object of the invention to provide kainic acid application in preparing anti-Cryptosporidium baileyi medicine.
Inventor, by by kainic acid and acceptable adjuvant, making powder, adds to feedstuff, and mixing, by daily
Forage volume feeding Lingnan Yellow chicken, addition is interpolation 4g kainic acid in every 100kg feedstuff；Or kainic acid is made water solublity
Powder, soluble in water by everyday water-drinking amount feeding white mice, addition is to be dissolved in 2g kainic acid in every 100kg water.By dynamic
Thing infection experiment confirms that adding kainic acid can make Cryptosporidium baileyi ovulation capsule amount reduce by more than 78%, has the most anti-hidden
Sporozoon effect.
Detailed description of the invention
Below in conjunction with specific embodiment and experiment, this explanation is further described, but limitation is not such.
Embodiment 1
The preparation of kainic acid powder: take 4g kainic acid (content is not less than 99%) and the mixing of 96g starch,.Red
Algae propylhomoserin is purchased from Merck company of Germany, article No.: 420318-10MGCN.
The usage and dosage of powder: above-mentioned 100g powder is admixed in 100 kg feedstuffs, by daily appetite feeding.
Embodiment 2
The preparation of kainic acid aqueous solvent: take 2 g kainic acids (content is not less than 99%) and the mixing of 98g glucose, i.e.
Can.
The usage and dosage of water solube powder: be dissolved in 200 liters of water by above-mentioned 100g powder, cuts off the water supply 2 to before medication sick chicken
Hour, then press normal water amount feeding.
Anti-Cryptosporidium baileyi below in conjunction with concrete description of test kainic acid is tested.
Experiment material:
Control drug: (98%, raw biotechnology is comforted in Shanghai to the paromomycin with anti-Cryptosporidium effect of document report
Company limited, lot number 001010), usage amount is 5mg/kg body weight.
Kainic acid: the kainic acid powder of embodiment 1, the kainic acid water solube powder of embodiment 2.
Cryptosporidium baileyi egg capsule: Cryptosporidium baileyi (C. baileyi) Guangdong Strain egg capsule, by Guangdong Agricultural science
Institute's animal health institute preserves, and (SPF chicken is purchased from Guangdong great Hua Nong inventory company limited, report in rejuvenation in SPF chicken body before using
Accuse and number: 20130216001, kind: BWEL-SPF chicken).
Chickling: Lingnan Yellow chickling, 1 age in days, male and female dual-purpose, Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences provide, raise
In the special Animal House sterilized；Chicken coop and the equal strict sterilization of vessel used, free choice feeding and drinking pure water.See before test
With or without clinical symptoms and 2d inspection feces is with or without Cryptosporidium parvum oocysts suspended continuously to examine chickling, standby.
Feedstuff: customized, by Guangdong Province Xin Nan all feed corporation,Ltds, material of brooding, without any anti-Cryptosporidium medicine.
Experimental technique:
Packet: by test packet and process by 150 Lingnan Yellow breeding chicklings to 3 ages in days, by only weighing, eliminate weak young bird and
The excessive person of body weight, is randomly divided into 5 groups by residue chickling, often group 30, and suitably adjusts that to make often to organize chickling TBW roughly equal.
Process: in addition to I (blank) group, remaining respectively organize chickling equal administered by oral gavage Cryptosporidium baileyi egg capsule l ×
106Individual/plumage；In addition to Group I and the IIth (positive control) group, remaining is respectively organized chickling and all feeds immediately or drink after infecting containing anti-
The feedstuff of Cryptosporidium medicine or water, the IIIth group feeds the feedstuff containing paromomycin, the IVth group of kainic acid feeding embodiment 1
The kainic acid spice that powder and feedstuff are made, the Vth group of kainic acid water solube powder drinking embodiment 2 is made with drinking-water
Kainic acid drinking-water, each group drinking-water is the most identical with the consumption of feedstuff.The packet situation of test chickling is shown in Table 1.
Chickling packet tested by table 1
。
Observe and record every day such as the mental status of chicken group, feed intake, feces situation etc.；Dead chickling is weighed, cuts open inspection；?
The weightening finish of each group of chickling of rear calculating and the price of deed.
Egg capsule slip after the infection of drug effect decision method Main Basis；It is carried out as follows: within after infection the 3rd day, start to collect every day
Feces, continuously monitoring 15 days.Taking feces every day to eluriate, 260 mesh screen, filtrate is centrifuged 10min through 3000r/min, abandons supernatant
Adding the saturated sucrose solution of 15mL, shake up, 1200/min is centrifuged 10min；Supernatant 20uL is the most carefully picked in red after Li Xin
On cell counting count board, microscope (10 × 40 times) is observed egg capsule and counts.
Experimental result:
Clinicing symptom observation:
The matched group of inoculation Cryptosporidium baileyi egg capsule the 3rd day, chickling occurs that spirit is depressed, by hair slightly disorderly, within the 4th day, plays abdomen
Rushing down symptom obvious, period has no that chickling is dead.
Calculate averagely every chickling row's egg sac number, egg capsule slip and the average weight of each group respectively, the results are shown in Table 2 institutes
Show.
Anti-Cryptosporidium effect respectively organized by table 2
As shown in Table 2, averagely every mice row's egg sac number of the III, the IV, V group is below the IIth group, and average body weight average is high
In Group II；Averagely every chickling row's egg sac number of the IV, the V group is significantly lower than the IIIth group, and average weight is higher than Group III.Therefore
Experiment confirms that kainic acid can make the metainfective egg sac number of Cryptosporidium baileyi reduce more than 78%, and the price of deed increases, drink
Water group effect is better than feedstuff interpolation group, has preferable anti-Cryptosporidium baileyi effect, and is substantially better than paromomycin.
The pharmaceutical salts of kainic acid or medicinal ester, hydrolyzable generates kainic acid in vivo, has same or like resisting
Cryptosporidium baileyi activity, it is also possible to as anti-Cryptosporidium baileyi drug use.The pharmaceutical salts of kainic acid can be its potassium,
Calcium, sodium, magnesium, aluminum, ferrum, zinc salt.The medicinal ester of kainic acid is the ester of itself C2～C4, particularly its ethyl ester, propyl ester or butyl ester.
Claims (2)
1. kainic acid and medicinal derivative application in preparing anti-Cryptosporidium baileyi medicine or feed additive, red algae
The medicinal derivative of propylhomoserin is its pharmaceutical salts or medicinal ester, and the pharmaceutical salts of kainic acid is its potassium, calcium, sodium, magnesium, aluminum, ferrum, zinc
Salt；The medicinal ester of kainic acid is the ester of itself C2～C4.
Application the most according to claim 1, it is characterised in that: C2～C4 ester is ethyl ester, propyl ester or butyl ester.
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| CN201510057560.5A CN104688729B (en) | 2015-02-04 | 2015-02-04 | Kainic acid application in preparing anti-Cryptosporidium baileyi medicine |
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| CN104688729B true CN104688729B (en) | 2016-11-30 |
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| CN103373948B (en) * | 2012-04-27 | 2015-07-08 | 中国科学院上海有机化学研究所 | Preparation method for natural product (-)-kainic acid |
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