CN105311007B - Application of the naganol in anticoccidial drugs thing is prepared - Google Patents
Application of the naganol in anticoccidial drugs thing is prepared Download PDFInfo
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- CN105311007B CN105311007B CN201510769252.5A CN201510769252A CN105311007B CN 105311007 B CN105311007 B CN 105311007B CN 201510769252 A CN201510769252 A CN 201510769252A CN 105311007 B CN105311007 B CN 105311007B
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Abstract
The invention discloses application of the naganol in anticoccidial drugs thing is prepared.Naganol is well mixed with acceptable auxiliary material, is added in feed, is effective against breeding of the Eimeria tenella in chicken body.Compared with conventional coccidiostat dinitolmide pre-mixing agent, anticoccidial effect is obvious.The present invention can effectively alleviate the serious drug resistance problems that current cultivation industry uses coccidiostat to produce, and can accelerate the research and development speed of new drug on this basis, and the green, sound development to aquaculture have important benefits.
Description
Technical field
The present invention relates to a kind of new application of naganol, and in particular to naganol is in anticoccidial drugs thing is prepared
Using.
Background technology
Chicken coccidiasis (Coccidiosis) it is most common a kind of parasitic protozoa disease in poultry production, by the multiple door Chinese mugwort in top
U.S. ear belongs to coccidia and parasitized caused by the intestinal epithelial cell of chicken.Chick within the monthly age of main harm 3, the death rate is up to 80%.
The chick growth of resistance to mistake is slow, and depauperation is generally carrier into chicken, and weightening is slow, and laying rate declines, the harm to poultry husbandry
It is extremely serious, huge economic loss is caused to poultry husbandry every year.It has been reported that 7 kinds of Eimerias in tender Amy
The pathogenicity of ear coccidia is most strong, and occurs extensive.The today increasingly improved in poultry husbandry intensive degree, chicken coccidiasis it is anti-
Control is also increasingly severeer.The use of coccidiostat and egg capsule vaccine living is the Main Means of current control chicken coccidiasis, still
There is the risk for dissipating poison in egg capsule vaccine living, therefore chemicals preventing and treating is still the important measures for controlling chicken coccidiasis.But mostly
Number coccidiostat is generally chemical classes product and antibioticses product, and the long-term use of these products causes anti-medicine very universal,
And the speed of new drug development is very slow, poultry husbandry is caused to face the available situation of no medicine, therefore in the urgent need to new anti-ball
Worm medicine appears on the market.
Naganol used of the invention（Suramin sodium）, alias：Suramin；Suramin Sodium；8,8'- phosphinylidynes are double sub-
Ammonia -3,1- phenylene phosphinylidyne imido-(4- methyl -3,1- phenylene) phosphinylidyne imido double -1, the sodium of 3,5- naphthalene trisulfonic acid six, CAS is stepped on
Record number：129-46-4, molecular formula：C51H34N6Na6O23S6, molecular weight：1429.17, its chemical constitution such as formula is such as（Ⅰ）It is shown：
（Ⅰ）.
Naganol is it is verified that with anti-trypanosomiasis, antitumor, preventing and treating eye proliferative diseases are antiviral, are immunized
The effect of conditioning agent, and be widely used in clinical practice.
Not yet there is naganol that there is the report of anti-Eimeria species effect at present, can not be pushed away by property known to naganol
Breaking, whether it has the effect of anti-Eimeria species.
The content of the invention
It is an object of the invention to provide application of the naganol in anti-Eimeria species
The technical solution used in the present invention is：
Naganol and its medicinal derivative are preparing the application of anticoccidial drugs thing.
Further, above-mentioned naganol medicinal derivative be the pharmaceutical salts of naganol, the hydrate of naganol, Soviet Union
The solvate of Lamine sodium.
Further, the pharmaceutical salts of above-mentioned naganol are the hydrochloride of naganol.
Further, said medicine formulation is oral formulations.
The application of naganol and its medicinal derivative in feed addictive is prepared.
Further, above-mentioned naganol medicinal derivative be the pharmaceutical salts of naganol, the hydrate of naganol, Soviet Union
The solvate of Lamine sodium.
Further, the pharmaceutical salts of above-mentioned naganol are the hydrochloride of naganol.
The beneficial effects of the invention are as follows：
The present invention is by by naganol or its medicinal derivative and acceptable auxiliary material, being made powder, afterwards by powder
Stirred added in feed.The addition of naganol is to add 2.5g per in 100kg feeds, using added with suramin
The feed of sodium presses daily forage volume feeding chickling；Being tested by Chickens Infected Eimeria tenella confirms the anti-ball of naganol
Worm index（ACI）It can reach more than 180, it was demonstrated that there is good resisting Eimeria tenella to act on for it.
Embodiment
Naganol and its medicinal derivative are preparing the application of anticoccidial drugs thing.
It is preferred that, above-mentioned naganol medicinal derivative is the pharmaceutical salts of naganol, the hydrate of naganol, Ursula
The solvate of natulanar.
It is preferred that, the pharmaceutical salts of above-mentioned naganol are the hydrochloride of naganol.
It is preferred that, said medicine formulation is oral formulations.
The application of naganol and its medicinal derivative in feed addictive is prepared.
It is preferred that, above-mentioned naganol medicinal derivative is the pharmaceutical salts of naganol, the hydrate of naganol, Ursula
The solvate of natulanar.
It is preferred that, the pharmaceutical salts of above-mentioned naganol are the hydrochloride of naganol.
With reference to specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
The preparation of naganol powder：Take 2.5g naganols（Content is not less than 99%）It is mixed with 97.5g soluble starches
It is even, you can.
The usage and dosage of powder：Above-mentioned 100g powders are admixed in 100 kg feeds, per diem often to eat amount feeding.
Embodiment 2
The preparation of naganol water solube powder：Take 2.5g naganols（Content is not less than 99%）With 97.5g glucose
Mix, you can.
The usage and dosage of water solube powder：Above-mentioned 100g pulvis is dissolved in 100 liters of water, by normal water amount feeding.
Embodiment 3
The preparation of naganol medicinal derivative water solube powder：Take 2.5g naganol medicinal derivatives（Content is not low
In 99%）Mixed with 97.5g glucose, you can.
The usage and dosage of water solube powder：Above-mentioned 100g pulvis is dissolved in 100 liters of water, by normal water amount feeding.
Below using naganol as representative, with reference to specific description of test naganol and its anti-chicken ball of medicinal derivative
Worm acts on.
Experiment material
1. control drug：Conventional coccidiostat dinitolmide pre-mixing agent, by Guangdong Qianyan Animal Health Ltd. Company
Production.
2. naganol：The naganol powder of embodiment 1, the naganol water solube powder of embodiment 2.
3. chicken coccidial oocyst：Eimeria tenella (Eimeria tenella) Guangdong Strain Sporulated Oocysts, by Guangdong Province
Animal health research institute of Academy of Agricultural Sciences preserves, using the preceding rejuvenation in non-ball worm Chickens, gathers caecum, collects egg capsule, puts
In 28 DEG C of water-bath miospores, when Sporulated rate is up to more than 80%, egg capsule is counted, 4 DEG C of refrigerators are put in standby.
4. chick：1 age in days Lingnanhuang broilers, are provided by animal science research institute of Guangdong Academy of Agricultural Sciences, raised in
In sterilization and the special Animal House of non-ball worm；Chicken coop and the equal strict sterilization of hopper, tank, free choice feeding and drinking pure water；Examination
Observation chick whether there is chicken coccidiasis clinical symptoms before testing and continuous 2d checks excrement whether there is coccidian oocyst, if any symptom and egg capsule then
Eliminate, it is standby if asymptomatic and egg capsule.
5. feed：It is special by animal science research institute of Guangdong Academy of Agricultural Sciences, without any coccidiostat.
Experimental method
Packet：By 10 age in days, 200 Lingnanhuang broilers by only weighing, the weak young and excessive person of body weight is eliminated, body weight is uniform
Remaining chick be randomly divided into 5 groups, every group 30, and it is roughly equal to adjust every group of chick total weight；
Processing：In addition to Group I (blank control), the equal administered by oral gavage Eimeria tenella Sporulated ovum of remaining each group chick
Capsule l × 105Individual/plumage；In addition to Group I and the IIth group (positive control), remaining each group chick is in feeding immediately after infection containing anti-
The feed of coccidia medicine, is continued until off-test.The feed of IIIth group of feeding dinitolmide containing coccidiostat pre-mixing agent,
The naganol spice that the naganol powder of IVth group of feeding embodiment 1 is made with feed, the Vth group drink embodiment 2 Soviet Union
Lamine sodium water solube powder is drunk water with the naganol that drinking-water is made, the consumption all same of each group drinking-water and feed.Test chick
Packet situation be shown in Table 1.
Mental status, feed intake, excrement situation of chicken group etc. are observed and recorded daily；Dead chick is weighed, cut open inspection is seen
Lesion is examined, lesion score is+4 points if dead because of infection Eimeria tenella；All chick in the 7.5th day after infection by
Only weigh, cut open inspection carries out caecum lesion and scored；Finally calculate weightening and the price of deed of each group chick.Test the packet of chick
Situation is shown in Table 1.
The experiment chick packet of table 1
The formula that drug effect decision method and standard anticoccidial index (ACI) are recommended by United States Merck company is calculated：ACI=(phase
To rate of body weight gain+survival rate) one (oocyst value+pathological changes value)；Body weight increase rate=(weightening of test group weightening ÷ blank control groups) ×
100%；Survival rate=(each group live chick number ÷ each groups chick sum) × 100%.Lesion is scored by Pyatyi：1. it is blind without egg capsule
Intestines are normal, are 0 point；2. there is egg capsule, caecum mucous membrane is slightly thickened, have and be dispersed in bleeding or a small amount of blood sample intestinal contents on a small quantity, be+1 point；
3. there is egg capsule, caecum mucous membrane is thickened, have obvious bleeding or obvious blood sample intestinal contents, be+2 points；4. there is egg capsule, caecum mucous membrane increases
Thickness, has a large amount of sludged bloods or blood sample intestines core, is+3 points；5. chick is because global-worm illness is dead or has a large amount of egg capsules, and caecum outward appearance is in sauce
Oil colours (or having point-like necrosis region in the middle part of small intestine, mucous membrane face is in blush), the obvious enlargement of intestinal tube, content forms obvious blood sample
Intestines core, is+4 points.Pathological changes value=every group of average lesion scores × 20 in group.Drug effect criterion is ACI<120 is invalid, 120-
160 be weak effect, and 160-180 be middle effect, and more than 180 be potent.Oocyst value by cecal content gram egg sac number (OPG) convert and
Come.
Experimental result：
Clinicing symptom observation:
Group I is not inoculated with Eimeria tenella Sporulated Oocysts, and chicken spirit is good, and appetite is normal, and no bloody stool occurs.
Group II is inoculated with after Eimeria tenella Sporulated Oocysts the 2nd day, and chicken appearance spirit is depressed, stays vertical, appetite subtracts
The symptom such as move back；Start within 5th day obvious bloody stool occur, concurrent life and death is died, and subsequent bloody stool is gradually decreased.
Anticoccidial index is calculated：
Cut open inspection chicken, takes caecum, is scored according to lesion, body weight increase rate, survival rate etc. calculate each group anticoccidial and referred to respectively
Number, the results are shown in Table shown in 2.
The each group anticoccidial index of table 2
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (3)
1. naganol is preparing the application of anticoccidial drugs thing.
2. apply according to claim 1, it is characterised in that：The pharmaceutical dosage form is oral formulations.
3. application of the naganol in the feed addictive for preparing anti-Eimeria species.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008057253A2 (en) * | 2006-10-27 | 2008-05-15 | Bioresponse, L.L.C. | Anti-parasitic methods and compositions utilizing diindolylmethane-related indoles |
| CN103142566A (en) * | 2013-02-25 | 2013-06-12 | 中国科学院生物物理研究所 | Important-protein inhibitor in severe fever with thrombocytopenia syndrome |
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2015
- 2015-11-12 CN CN201510769252.5A patent/CN105311007B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008057253A2 (en) * | 2006-10-27 | 2008-05-15 | Bioresponse, L.L.C. | Anti-parasitic methods and compositions utilizing diindolylmethane-related indoles |
| CN103142566A (en) * | 2013-02-25 | 2013-06-12 | 中国科学院生物物理研究所 | Important-protein inhibitor in severe fever with thrombocytopenia syndrome |
Non-Patent Citations (1)
| Title |
|---|
| 中国家畜伊氏锥虫病研究进展;王光雷等;《草食家畜》;19950630;第87卷(第2期);第52至56页 * |
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